BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Aconitum21_contig00012221
         (1294 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   542   e-152
sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coc...   541   e-151
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra...   533   e-149
dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransfe...   459   e-127
gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   441   e-121

>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
            4'-O-methyltransferase [Coptis chinensis]
          Length = 350

 Score =  542 bits (1397), Expect = e-152
 Identities = 270/351 (76%), Positives = 308/351 (87%), Gaps = 4/351 (1%)
 Frame = +3

Query: 15   MPFHDSHEPADIKAQAHVWKLIYGFADSLVLRCAVELQIPDIIHSNNNQPMDLADIASKL 194
            M FH   +  DIKAQAHVWK+IYGFADSLVLRCAVEL I DI+  NNNQPM LAD+ASKL
Sbjct: 1    MAFHGKDDALDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIV-DNNNQPMALADLASKL 59

Query: 195  HIPNVNPNHLYRILRYLVKMEILKLH----GVTQYTLNPIATLLLRNQKRNMVPIILGVT 362
             + NVN ++LYRILRYLVKMEIL++     G  +Y L PIATLL RN KR+MVP+ILG+T
Sbjct: 60   PVSNVNCDNLYRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGMT 119

Query: 363  QKDFMTPWHYMKDGISNNGTAFEKAMGMSLWEYLEGRADQSLLFNEGMAGETRLLTEELV 542
            QKDFMTPWH MKDG+S+NGTAFEKAMGM++WEYLEG  DQS LFNEGMAGETRLLT  L+
Sbjct: 120  QKDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLI 179

Query: 543  LGCRDVFENIESLVDVGGGNGTTIKAISDAFPNVKCTLFDLPHVIASSCEVPGVERIGGD 722
             G RD+F+ I+SLVDVGGGNGTT+KAISDAFP++KCTLFDLPHVIA+S + P +ERIGGD
Sbjct: 180  SGSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGD 239

Query: 723  MFEFMPSAQAILLKLILHDWSDEESIKILKQCKAAVPEKGGKVIIVDVALDEESEHELSA 902
            MF+ +PSAQAILLKLILHDW+DE+SIKILKQC+ AVP+ GGKVIIVDVALDEES+HELS+
Sbjct: 240  MFKSVPSAQAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSS 299

Query: 903  TRLILDVDMLVNTGGKERSKEDWEKLVKCAGFSGCNIRHIAAIQSVIEVFP 1055
            TRLILD+DMLVNTGGKER+KE WEK+VK AGFSGC IRHIAAIQSVIEVFP
Sbjct: 300  TRLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350


>sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
            4'-O-methyltransferase; AltName:
            Full=S-adenosyl-L-methionine:3'-hydroxy-N-
            methylcoclaurine 4'-O-methyltransferase; Short=4'-OMT
            gi|9711916|dbj|BAB08005.1|
            S-adenosyl-L-methionine:3'-hydroxy-N-methylcocla urine
            4'-O-methyltransferase [Coptis japonica]
          Length = 350

 Score =  541 bits (1394), Expect = e-151
 Identities = 269/351 (76%), Positives = 309/351 (88%), Gaps = 4/351 (1%)
 Frame = +3

Query: 15   MPFHDSHEPADIKAQAHVWKLIYGFADSLVLRCAVELQIPDIIHSNNNQPMDLADIASKL 194
            M FH   +  DIKAQAHVWK+IYGFADSLVLRCAVEL I DII  NNNQPM LAD+ASKL
Sbjct: 1    MAFHGKDDVLDIKAQAHVWKIIYGFADSLVLRCAVELGIVDII-DNNNQPMALADLASKL 59

Query: 195  HIPNVNPNHLYRILRYLVKMEILKLH----GVTQYTLNPIATLLLRNQKRNMVPIILGVT 362
             + +VN ++LYRILRYLVKMEIL++     G  +Y L PIATLL RN KR+MVP+ILG+T
Sbjct: 60   PVSDVNCDNLYRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMT 119

Query: 363  QKDFMTPWHYMKDGISNNGTAFEKAMGMSLWEYLEGRADQSLLFNEGMAGETRLLTEELV 542
            QKDFMTPWH MKDG+S+NGTAFEKAMGM++WEYLEG  DQS LFNEGMAGETRLLT  L+
Sbjct: 120  QKDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLI 179

Query: 543  LGCRDVFENIESLVDVGGGNGTTIKAISDAFPNVKCTLFDLPHVIASSCEVPGVERIGGD 722
             G RD+F+ I+SLVDVGGGNGTT+KAISDAFP++KCTLFDLPHVIA+S ++P +ERIGGD
Sbjct: 180  SGSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGD 239

Query: 723  MFEFMPSAQAILLKLILHDWSDEESIKILKQCKAAVPEKGGKVIIVDVALDEESEHELSA 902
            MF+ +PSAQAI+LKLILHDW+DE+SIKILKQC+ AVP+ GGKVIIVDVALDEES+HELS+
Sbjct: 240  MFKSVPSAQAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSS 299

Query: 903  TRLILDVDMLVNTGGKERSKEDWEKLVKCAGFSGCNIRHIAAIQSVIEVFP 1055
            TRLILD+DMLVNTGGKER+KE WEK+VK AGFSGC IRHIAAIQSVIEVFP
Sbjct: 300  TRLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350


>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase [Thalictrum
            flavum subsp. glaucum]
          Length = 348

 Score =  533 bits (1372), Expect = e-149
 Identities = 265/346 (76%), Positives = 305/346 (88%), Gaps = 1/346 (0%)
 Frame = +3

Query: 21   FHDSHEPADIKAQAHVWKLIYGFADSLVLRCAVELQIPDIIHSNNNQPMDLADIASKLHI 200
            F       DIKAQAHVWK+IYGFADSLVLRCAVEL I DII  NNNQP+ LAD+ASKL I
Sbjct: 4    FQGKDASVDIKAQAHVWKIIYGFADSLVLRCAVELGIFDII-DNNNQPIPLADLASKLPI 62

Query: 201  PNVNPNHLYRILRYLVKMEILKLHGVTQYTLNPIATLLLRNQKRNMVPIILGVTQKDFMT 380
             NVN ++L+RILRYLVKME+L      +Y L PIA L+LRN+KR+MVP++LG+TQKDFMT
Sbjct: 63   SNVNLDNLFRILRYLVKMELLSYAADDKYALEPIAKLVLRNEKRSMVPMVLGMTQKDFMT 122

Query: 381  PWHYMKDGISNNG-TAFEKAMGMSLWEYLEGRADQSLLFNEGMAGETRLLTEELVLGCRD 557
            PWH MKDG+++NG TAFEKAMGM++WEYLEG  DQS LFNEGMAGETRLLT  L+ G +D
Sbjct: 123  PWHSMKDGLTDNGVTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSKD 182

Query: 558  VFENIESLVDVGGGNGTTIKAISDAFPNVKCTLFDLPHVIASSCEVPGVERIGGDMFEFM 737
            +F++I+SLVDVGGGNGTTIKAIS AFP++KCTLFDLPHVIA+S + P +ERIGGDMF+ M
Sbjct: 183  MFQSIDSLVDVGGGNGTTIKAISVAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFKGM 242

Query: 738  PSAQAILLKLILHDWSDEESIKILKQCKAAVPEKGGKVIIVDVALDEESEHELSATRLIL 917
            PSAQAILLKLILHDW+DE+SIKILKQC+ AVP+ GGKVIIVDVALDE+S+HELS+TRLIL
Sbjct: 243  PSAQAILLKLILHDWNDEDSIKILKQCRKAVPKDGGKVIIVDVALDEDSDHELSSTRLIL 302

Query: 918  DVDMLVNTGGKERSKEDWEKLVKCAGFSGCNIRHIAAIQSVIEVFP 1055
            D+DMLVNTGGKER+KEDWEKLVKCAGFSGC IRHIAAIQSVIEVFP
Sbjct: 303  DIDMLVNTGGKERTKEDWEKLVKCAGFSGCKIRHIAAIQSVIEVFP 348


>dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase [Eschscholzia
            californica]
          Length = 353

 Score =  459 bits (1181), Expect = e-127
 Identities = 225/349 (64%), Positives = 283/349 (81%), Gaps = 8/349 (2%)
 Frame = +3

Query: 33   HEPADIKAQAHVWKLIYGFADSLVLRCAVELQIPDIIHSNNNQPMDLADIASKLHIPNVN 212
            +E  DIKAQAH+W +IYGFADSLVLR AVEL I DII  NNN  + ++++ASKL I NVN
Sbjct: 6    NEEVDIKAQAHLWNIIYGFADSLVLRSAVELGIADII-KNNNGSITVSELASKLPISNVN 64

Query: 213  PNHLYRILRYLVKMEILKLHGVT--------QYTLNPIATLLLRNQKRNMVPIILGVTQK 368
             ++LYR+LRYLV M ILK    T         Y+L P+ +LL+++ +RNMVPI+LG+TQ+
Sbjct: 65   SDNLYRVLRYLVHMGILKETKSTINGGEIKKLYSLEPVGSLLVKDAERNMVPIVLGMTQQ 124

Query: 369  DFMTPWHYMKDGISNNGTAFEKAMGMSLWEYLEGRADQSLLFNEGMAGETRLLTEELVLG 548
            DFM PWHY+K+G+    TAFEK MGM+LWEYLEG  +Q  LFN GM GETRLLT+ L+  
Sbjct: 125  DFMIPWHYIKEGLGEGSTAFEKGMGMTLWEYLEGHPEQGHLFNVGMEGETRLLTKTLIES 184

Query: 549  CRDVFENIESLVDVGGGNGTTIKAISDAFPNVKCTLFDLPHVIASSCEVPGVERIGGDMF 728
            C+D FE + SLVDVGGGNGTTIKAIS+AFP++KC+L+DLPHV+A S ++P +E+I GD+F
Sbjct: 185  CKDTFEGLSSLVDVGGGNGTTIKAISEAFPHIKCSLYDLPHVVADSHDLPNIEKIPGDIF 244

Query: 729  EFMPSAQAILLKLILHDWSDEESIKILKQCKAAVPEKGGKVIIVDVALDEESEHELSATR 908
            +F+P+AQAILLKLILHDWSDE+S+KILK+C+ AVP+  G+VIIVDVAL+EESEH L+ TR
Sbjct: 245  KFIPNAQAILLKLILHDWSDEDSVKILKKCREAVPQDTGRVIIVDVALEEESEHPLTKTR 304

Query: 909  LILDVDMLVNTGGKERSKEDWEKLVKCAGFSGCNIRHIAAIQSVIEVFP 1055
            L+LDVDMLVNTGG+ERS++DW KL+K AGF    IRHIAA+QSVIE FP
Sbjct: 305  LVLDVDMLVNTGGRERSEDDWAKLLKLAGFRTHKIRHIAAVQSVIEAFP 353


>gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
            4'-O-methyltransferase 2 [Papaver somniferum]
          Length = 357

 Score =  441 bits (1135), Expect = e-121
 Identities = 218/346 (63%), Positives = 279/346 (80%), Gaps = 6/346 (1%)
 Frame = +3

Query: 36   EPADIKAQAHVWKLIYGFADSLVLRCAVELQIPDIIHSNNNQPMDLADIASKLHIPNVNP 215
            +   IK QA +W +IYGFADSLVLRCAVE+ I DII  NN+  + LA +A+KL I NV+ 
Sbjct: 13   QEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADII-KNNDGAITLAQLAAKLPITNVSS 71

Query: 216  NHLYRILRYLVKMEILKLH----GVTQ-YTLNPIATLLLRNQKRNMVPIILGVTQKDFMT 380
            ++LYR++RYLV + I++      GV + Y+L P+ TLLLR+ +R+MVP+ILG+TQKDFM 
Sbjct: 72   DYLYRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMV 131

Query: 381  PWHYMKDGISNNGT-AFEKAMGMSLWEYLEGRADQSLLFNEGMAGETRLLTEELVLGCRD 557
             WH+MK+G+ N  T AFEK MGM +W+YLEG  DQS LFNEGMAGETRLLT+ L+  CRD
Sbjct: 132  SWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRD 191

Query: 558  VFENIESLVDVGGGNGTTIKAISDAFPNVKCTLFDLPHVIASSCEVPGVERIGGDMFEFM 737
             F+ ++SLVD+GGGNGTTIKAI +AFP++KCTL+DLPHV+A+S ++P +E++ GDMF+ +
Sbjct: 192  TFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSV 251

Query: 738  PSAQAILLKLILHDWSDEESIKILKQCKAAVPEKGGKVIIVDVALDEESEHELSATRLIL 917
            PSAQAILLKLILHDW+DEE + ILK+CK A+P++ GKVIIVDVAL+EES HEL+ TRLIL
Sbjct: 252  PSAQAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLIL 311

Query: 918  DVDMLVNTGGKERSKEDWEKLVKCAGFSGCNIRHIAAIQSVIEVFP 1055
            D+DMLVNTGG+ER+ +DWE L+K AGF    IR I AIQSVIE FP
Sbjct: 312  DIDMLVNTGGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357