BLASTX nr result
ID: Aconitum21_contig00009212
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Aconitum21_contig00009212
(1622 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004140317.1| PREDICTED: tRNA modification GTPase MnmE-lik... 625 0.0
ref|XP_003527915.1| PREDICTED: tRNA modification GTPase MnmE-lik... 627 0.0
ref|XP_002513878.1| gtpase mss1/trme, putative [Ricinus communis... 622 0.0
ref|XP_002285809.1| PREDICTED: tRNA modification GTPase MnmE [Vi... 647 0.0
ref|NP_001149416.1| LOC100283042 [Zea mays] gi|195627080|gb|ACG3... 615 0.0
>ref|XP_004140317.1| PREDICTED: tRNA modification GTPase MnmE-like [Cucumis sativus]
gi|449526047|ref|XP_004170026.1| PREDICTED: tRNA
modification GTPase MnmE-like [Cucumis sativus]
Length = 561
Score = 625 bits (1613), Expect(2) = 0.0
Identities = 314/423 (74%), Positives = 358/423 (84%)
Frame = -3
Query: 1371 WRPTSHFVEYGVVSDLGGNVVDEVLALPMLAPKSYTREDVVELQCHGNDVCLRRVLRACL 1192
WRPTSH VEYGVV D G+V+DEVL +PMLAP+SYTREDV+ELQCHG++VCLRRVL+ACL
Sbjct: 139 WRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIELQCHGSEVCLRRVLKACL 198
Query: 1191 EAGARLAEPGEFTLRAFLNGRLDLSQAENIEKLISXXXXXXXXXXXAGIQGGFSDLVKSL 1012
EAGARLAEPGEFTLRAFLNGRLDLSQAEN+ KLIS AGIQGGFS LVKSL
Sbjct: 199 EAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKSL 258
Query: 1011 RKQCIELLTEIEARLDFDDEMPSLDANSVKDMIHAMCIEVEKALETANYDKLLQSGLQIA 832
R QCIELLTEIEARLDFDDEMP LD N V + +HAM EVE ALETANYDKLLQSG+QIA
Sbjct: 259 RTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVETALETANYDKLLQSGIQIA 318
Query: 831 IVGRPNVGKSSLLNAWSKSEKAIVTEIAGTTRDVVEASINVHGIPITLLDTAGIRETDDV 652
IVGRPNVGKSSLLNAWSKSE+AIVTEIAGTTRDV+EA++ V GIP+TLLDTAGIRETDD+
Sbjct: 319 IVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDI 378
Query: 651 VEKIGVERSEAVAMGADVIIMTVSALDGWTSDDAELLQRIQVNQKTTSSSAPMILVINKV 472
VEKIGVERSEA A+GADVIIM +SALDGWT++D LL RI +K+ S P++LVINK+
Sbjct: 379 VEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILSKKKSDESCTPILLVINKI 438
Query: 471 DCAPSPSIEAFKSNDNPFKKQVLTCAVRGEGLPDLESALAEIVGLDSIPARGRRWAVNQR 292
DCAPSP ++A N + F KQV TCAV G+G+ +LE A++E+VGL+ A GRRW VNQR
Sbjct: 439 DCAPSPKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQR 498
Query: 291 QFEQLVRTKEALLRLKSSIEEDIPLDFWTIDLREAALALGQISGEDISEEILSNIFSKFC 112
Q QL+RTKEA RLKSSIE+++P DFWT+DLR+A LALG+I GEDISEEILSNIF KFC
Sbjct: 499 QCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEICGEDISEEILSNIFGKFC 558
Query: 111 IGK 103
IGK
Sbjct: 559 IGK 561
Score = 60.5 bits (145), Expect(2) = 0.0
Identities = 36/67 (53%), Positives = 42/67 (62%), Gaps = 6/67 (8%)
Frame = -2
Query: 1561 HSTPKGETPTLREEEKLGSN------VHIEKSSTIAAIVTSLGTQPGAVGIVRLSGPSAV 1400
HST K T L +E+LG + I+ SSTIAAIVTS+G P AVGIVRLSGP AV
Sbjct: 57 HSTGKENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAV 116
Query: 1399 EIARRVF 1379
I +F
Sbjct: 117 NIVGTLF 123
>ref|XP_003527915.1| PREDICTED: tRNA modification GTPase MnmE-like [Glycine max]
Length = 548
Score = 627 bits (1617), Expect(2) = 0.0
Identities = 320/424 (75%), Positives = 360/424 (84%), Gaps = 1/424 (0%)
Frame = -3
Query: 1371 WRPTSHFVEYGVVSDLGGNVVDEVLALPMLAPKSYTREDVVELQCHGNDVCLRRVLRACL 1192
WRP SH VEYGVV D GNV+DEVLA+PMLAP+SYTREDVVELQCHG++VCLRRVLR CL
Sbjct: 125 WRPASHVVEYGVVLDSDGNVIDEVLAVPMLAPRSYTREDVVELQCHGSEVCLRRVLRTCL 184
Query: 1191 EAGARLAEPGEFTLRAFLNGRLDLSQAENIEKLISXXXXXXXXXXXAGIQGGFSDLVKSL 1012
EAGA LA+PGEFTLRAFLNGRLDLSQAEN+ +LI+ GIQGGFS LV+SL
Sbjct: 185 EAGATLAQPGEFTLRAFLNGRLDLSQAENVARLIAAKSVAAADAALEGIQGGFSSLVRSL 244
Query: 1011 RKQCIELLTEIEARLDFDDEMPSLDANSVKDMIHAMCIEVEKALETANYDKLLQSGLQIA 832
R QCIELLTEIEARLDFDDEMP LD N D IH M EVE ALETANYDKLLQSGLQIA
Sbjct: 245 RSQCIELLTEIEARLDFDDEMPPLDLNLTMDKIHNMSREVENALETANYDKLLQSGLQIA 304
Query: 831 IVGRPNVGKSSLLNAWSKSEKAIVTEIAGTTRDVVEASINVHGIPITLLDTAGIRETDDV 652
IVGRPNVGKSSLLNAWSKSE+AIVTEIAGTTRDV+EASI+V GIPITLLDTAGIR+TDD+
Sbjct: 305 IVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEASISVSGIPITLLDTAGIRDTDDI 364
Query: 651 VEKIGVERSEAVAMGADVIIMTVSALDGWTSDDAELLQRIQVNQKTTSSSAPMILVINKV 472
VEKIGVERSEAVA GAD+IIMTVSA++GWTS+D +LL+RIQ + +T SS P+ILV+NK+
Sbjct: 365 VEKIGVERSEAVARGADLIIMTVSAVEGWTSEDTKLLERIQSTKGSTGSSTPVILVVNKI 424
Query: 471 DCAPSPSIEAFKS-NDNPFKKQVLTCAVRGEGLPDLESALAEIVGLDSIPARGRRWAVNQ 295
DC P + K + F K+V TCAV G+GL DLE A+ +IVGL+ IPA GRRW VNQ
Sbjct: 425 DCKPCAETKWDKGCQSHIFSKRVFTCAVTGQGLHDLERAVLQIVGLEGIPAGGRRWTVNQ 484
Query: 294 RQFEQLVRTKEALLRLKSSIEEDIPLDFWTIDLREAALALGQISGEDISEEILSNIFSKF 115
RQ EQLVRTKEAL RL+SSI+E++PLDFWTIDLR+AAL+LGQISGEDISEE+LSNIF KF
Sbjct: 485 RQCEQLVRTKEALARLQSSIKEELPLDFWTIDLRDAALSLGQISGEDISEEVLSNIFGKF 544
Query: 114 CIGK 103
CIGK
Sbjct: 545 CIGK 548
Score = 55.1 bits (131), Expect(2) = 0.0
Identities = 32/74 (43%), Positives = 48/74 (64%), Gaps = 5/74 (6%)
Frame = -2
Query: 1585 PLIPTKSLHSTPKGETPTL--REEEKLGSNVHIEK---SSTIAAIVTSLGTQPGAVGIVR 1421
P P++++ S + L +++E+LG+ +++ +TIAAIVTS+G P AVGIVR
Sbjct: 45 PSKPSRTVKSRASSDNYNLVLKKDERLGTGECVDEVASGTTIAAIVTSVGGPPAAVGIVR 104
Query: 1420 LSGPSAVEIARRVF 1379
LSGP AV I R+F
Sbjct: 105 LSGPGAVSIVGRLF 118
>ref|XP_002513878.1| gtpase mss1/trme, putative [Ricinus communis]
gi|223546964|gb|EEF48461.1| gtpase mss1/trme, putative
[Ricinus communis]
Length = 557
Score = 622 bits (1604), Expect(2) = 0.0
Identities = 313/423 (73%), Positives = 359/423 (84%)
Frame = -3
Query: 1371 WRPTSHFVEYGVVSDLGGNVVDEVLALPMLAPKSYTREDVVELQCHGNDVCLRRVLRACL 1192
W+PTSH V+YGV D GNV+DEVLALPML+P+SYT EDVVELQCHG++VCL RVLRACL
Sbjct: 135 WQPTSHVVDYGVALDNEGNVIDEVLALPMLSPRSYTCEDVVELQCHGSEVCLTRVLRACL 194
Query: 1191 EAGARLAEPGEFTLRAFLNGRLDLSQAENIEKLISXXXXXXXXXXXAGIQGGFSDLVKSL 1012
+AGARLAEPGEFTLRAFLNGR+DLSQAEN+ KLIS AG+QGGF+ L+KSL
Sbjct: 195 QAGARLAEPGEFTLRAFLNGRVDLSQAENVGKLISANSVTAADAALAGLQGGFASLIKSL 254
Query: 1011 RKQCIELLTEIEARLDFDDEMPSLDANSVKDMIHAMCIEVEKALETANYDKLLQSGLQIA 832
R QCIELLTEIEARLDFDDEMP LD N + D IH M ++E ALETANYDKLLQSGLQIA
Sbjct: 255 RAQCIELLTEIEARLDFDDEMPPLDLNLIVDRIHVMSQDIEIALETANYDKLLQSGLQIA 314
Query: 831 IVGRPNVGKSSLLNAWSKSEKAIVTEIAGTTRDVVEASINVHGIPITLLDTAGIRETDDV 652
+VGRPNVGKSSLLNAWSKSE+AIVTEIAGTTRDVVEA + V GIP+TLLDTAGIRETDD+
Sbjct: 315 LVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVVEAGVTVGGIPVTLLDTAGIRETDDI 374
Query: 651 VEKIGVERSEAVAMGADVIIMTVSALDGWTSDDAELLQRIQVNQKTTSSSAPMILVINKV 472
VEKIGVERSEAVAMGADVII+T+SA DGWTS+D+ELL RI+ N+K+ SS P++L INK+
Sbjct: 375 VEKIGVERSEAVAMGADVIILTISAFDGWTSEDSELLSRIESNKKSVGSSTPVVLAINKI 434
Query: 471 DCAPSPSIEAFKSNDNPFKKQVLTCAVRGEGLPDLESALAEIVGLDSIPARGRRWAVNQR 292
D APS S+E F K V TCAV G+G+ +LE A++EIVGL+ IP GR+W VNQR
Sbjct: 435 DTAPSLSMEWIGRYSKAFSKHVFTCAVTGQGIKELEMAISEIVGLNRIPTGGRKWTVNQR 494
Query: 291 QFEQLVRTKEALLRLKSSIEEDIPLDFWTIDLREAALALGQISGEDISEEILSNIFSKFC 112
Q EQL+RTKEAL RLKSSI++++PLDFWTIDLR+AALALGQISGE ISEE+LSNIF KFC
Sbjct: 495 QCEQLMRTKEALARLKSSIKDEMPLDFWTIDLRDAALALGQISGEGISEEVLSNIFGKFC 554
Query: 111 IGK 103
IGK
Sbjct: 555 IGK 557
Score = 57.4 bits (137), Expect(2) = 0.0
Identities = 38/79 (48%), Positives = 49/79 (62%), Gaps = 3/79 (3%)
Frame = -2
Query: 1606 SLTPFPKPLIPTKSLHSTPKGETPTLREEEKLGSNVHIEKSS---TIAAIVTSLGTQPGA 1436
+L P P P PT L++ +++ LGSN + + TIAAIVTS+G P A
Sbjct: 55 TLNPNPNPK-PTLFLNN---------KDQRFLGSNGSSDNDNSGTTIAAIVTSVGGPPAA 104
Query: 1435 VGIVRLSGPSAVEIARRVF 1379
VGIVRLSGPSAV++A RVF
Sbjct: 105 VGIVRLSGPSAVDVAARVF 123
>ref|XP_002285809.1| PREDICTED: tRNA modification GTPase MnmE [Vitis vinifera]
gi|302141924|emb|CBI19127.3| unnamed protein product
[Vitis vinifera]
Length = 571
Score = 647 bits (1669), Expect = 0.0
Identities = 336/443 (75%), Positives = 370/443 (83%), Gaps = 5/443 (1%)
Frame = -3
Query: 1416 RVRRPWRLR-----GGCLDCWRPTSHFVEYGVVSDLGGNVVDEVLALPMLAPKSYTREDV 1252
RV RP R G WRPTSH VEYGVV D GNVVDEVLA+PMLAP+SYTREDV
Sbjct: 130 RVFRPARRNKGKTLGSGSGSWRPTSHVVEYGVVLDHHGNVVDEVLAIPMLAPRSYTREDV 189
Query: 1251 VELQCHGNDVCLRRVLRACLEAGARLAEPGEFTLRAFLNGRLDLSQAENIEKLISXXXXX 1072
VELQCHG++VCLRRVLRACLE+GARLAEPGEFTLRAFLNGRLDLSQAEN+ KLIS
Sbjct: 190 VELQCHGSEVCLRRVLRACLESGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSIA 249
Query: 1071 XXXXXXAGIQGGFSDLVKSLRKQCIELLTEIEARLDFDDEMPSLDANSVKDMIHAMCIEV 892
AGIQGGFS LV+SLR QCIELLTEIEARLDFDDEMP LD N + D IH+M +V
Sbjct: 250 AADAALAGIQGGFSSLVRSLRIQCIELLTEIEARLDFDDEMPPLDLNLIMDKIHSMSQDV 309
Query: 891 EKALETANYDKLLQSGLQIAIVGRPNVGKSSLLNAWSKSEKAIVTEIAGTTRDVVEASIN 712
E ALETANYD+LLQSGLQIAI+GRPNVGKSSLLNAWSKSE+AIVTEIAGTTRDVVEAS++
Sbjct: 310 ENALETANYDQLLQSGLQIAIIGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVVEASVS 369
Query: 711 VHGIPITLLDTAGIRETDDVVEKIGVERSEAVAMGADVIIMTVSALDGWTSDDAELLQRI 532
+HGIP+TLLDTAGIRETDD+VEKIGVERSEAVA+ ADVIIMT+SALDGWTS D +L RI
Sbjct: 370 IHGIPVTLLDTAGIRETDDIVEKIGVERSEAVAISADVIIMTISALDGWTSQDTKLFNRI 429
Query: 531 QVNQKTTSSSAPMILVINKVDCAPSPSIEAFKSNDNPFKKQVLTCAVRGEGLPDLESALA 352
N+K SS P+ILV+NK+DCAPS E F N F K + TCAV G+G+ DLESA+
Sbjct: 430 ISNKKFVESSTPVILVVNKIDCAPSACTELFMEG-NSFSKHIFTCAVTGQGISDLESAII 488
Query: 351 EIVGLDSIPARGRRWAVNQRQFEQLVRTKEALLRLKSSIEEDIPLDFWTIDLREAALALG 172
EIVGL+ IPA GRRW VNQRQ EQLVRTKEAL RL SSIEE++PLDFWTIDLREAALALG
Sbjct: 489 EIVGLNKIPAGGRRWTVNQRQCEQLVRTKEALARLISSIEEEMPLDFWTIDLREAALALG 548
Query: 171 QISGEDISEEILSNIFSKFCIGK 103
QISGEDISEE+L+NIF KFCIGK
Sbjct: 549 QISGEDISEEVLTNIFGKFCIGK 571
Score = 72.4 bits (176), Expect = 3e-10
Identities = 48/86 (55%), Positives = 55/86 (63%), Gaps = 9/86 (10%)
Frame = -2
Query: 1609 PSLTPF---PKPLIPTKSLHSTPKGETPTLREEEKLGSNV----HIEKSST--IAAIVTS 1457
PSL P+ K L+P LH TP T L+++E+LGS HIE +ST IAAIVTS
Sbjct: 49 PSLKPYHTHSKTLVP--HLHLTPNDTTLVLKKDERLGSRSVNQEHIEGTSTTTIAAIVTS 106
Query: 1456 LGTQPGAVGIVRLSGPSAVEIARRVF 1379
LG P AVGIVRLSGP AV I RVF
Sbjct: 107 LGGPPSAVGIVRLSGPEAVAIVARVF 132
>ref|NP_001149416.1| LOC100283042 [Zea mays] gi|195627080|gb|ACG35370.1| tRNA modification
GTPase trmE [Zea mays]
Length = 546
Score = 615 bits (1585), Expect(2) = 0.0
Identities = 301/423 (71%), Positives = 359/423 (84%)
Frame = -3
Query: 1371 WRPTSHFVEYGVVSDLGGNVVDEVLALPMLAPKSYTREDVVELQCHGNDVCLRRVLRACL 1192
W+P SHFVEYG+ D G+V+DEVL +PML+P+SYTREDVVELQCHGND+CLRR+LRACL
Sbjct: 124 WQPRSHFVEYGLALDADGSVIDEVLVVPMLSPRSYTREDVVELQCHGNDLCLRRLLRACL 183
Query: 1191 EAGARLAEPGEFTLRAFLNGRLDLSQAENIEKLISXXXXXXXXXXXAGIQGGFSDLVKSL 1012
EAGARLA+PGEFTLRAFLNGRLDL+QAEN+ +LIS AGIQGGFS LVKSL
Sbjct: 184 EAGARLADPGEFTLRAFLNGRLDLAQAENVSRLISAKSAAAADSALAGIQGGFSTLVKSL 243
Query: 1011 RKQCIELLTEIEARLDFDDEMPSLDANSVKDMIHAMCIEVEKALETANYDKLLQSGLQIA 832
R +CIELLTEIEARLDF+DEMP LD + I+ M EV+ AL+T+NYDKLLQSGLQIA
Sbjct: 244 RSRCIELLTEIEARLDFEDEMPPLDPVMLVSKINCMRKEVQDALDTSNYDKLLQSGLQIA 303
Query: 831 IVGRPNVGKSSLLNAWSKSEKAIVTEIAGTTRDVVEASINVHGIPITLLDTAGIRETDDV 652
I+GRPNVGKSSLLNAWSKSE+AIVTEIAGTTRDVVEA++++HG+P+TLLDTAGIRETDDV
Sbjct: 304 IIGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVVEANVSIHGVPVTLLDTAGIRETDDV 363
Query: 651 VEKIGVERSEAVAMGADVIIMTVSALDGWTSDDAELLQRIQVNQKTTSSSAPMILVINKV 472
VEKIGV+RSEA AMGAD+I+M +SA+DGWT DD +L++ + VN+K++ S+ PM+LVINKV
Sbjct: 364 VEKIGVKRSEAAAMGADLIVMAISAVDGWTDDDTKLMEHVSVNRKSSGSAVPMVLVINKV 423
Query: 471 DCAPSPSIEAFKSNDNPFKKQVLTCAVRGEGLPDLESALAEIVGLDSIPARGRRWAVNQR 292
DCAP E FK F+K V TCAV G+G+ DLESA+ E+ G++ +P+ GRRW VNQR
Sbjct: 424 DCAPFVPGEQFKQFSGLFRKHVQTCAVTGKGISDLESAVIEVRGIEHVPSEGRRWTVNQR 483
Query: 291 QFEQLVRTKEALLRLKSSIEEDIPLDFWTIDLREAALALGQISGEDISEEILSNIFSKFC 112
QFEQL+RTKEA RL+SSI E +P+DFWT+DLREAALAL ISGEDISEE+LS+IFSKFC
Sbjct: 484 QFEQLLRTKEAFARLESSINEQLPMDFWTVDLREAALALATISGEDISEEVLSSIFSKFC 543
Query: 111 IGK 103
IGK
Sbjct: 544 IGK 546
Score = 52.4 bits (124), Expect(2) = 0.0
Identities = 27/35 (77%), Positives = 29/35 (82%)
Frame = -2
Query: 1483 STIAAIVTSLGTQPGAVGIVRLSGPSAVEIARRVF 1379
STIAAIVTSLG P AVGIVR+SGP AV +A RVF
Sbjct: 78 STIAAIVTSLGGGPAAVGIVRISGPDAVAVADRVF 112