BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Aconitum21_contig00007250
         (1144 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precurso...   360   e-115
ref|XP_002311286.1| ent-kaurene synthase [Populus trichocarpa] g...   333   e-108
gb|AFG18184.1| ent-kaurene synthase [Malus x domestica]               333   e-107
gb|AEN74904.1| ent-kaurene synthase [Pyrus communis]                  331   e-107
ref|XP_003532911.1| PREDICTED: ent-kaur-16-ene synthase, chlorop...   329   e-105

>ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precursor, putative [Ricinus
            communis] gi|223526405|gb|EEF28689.1| Ent-kaurene
            synthase B, chloroplast precursor, putative [Ricinus
            communis]
          Length = 808

 Score =  360 bits (924), Expect(2) = e-115
 Identities = 182/289 (62%), Positives = 220/289 (76%)
 Frame = -3

Query: 869  WLMKDEEIFLETATCAIAFRILRAHGYDVSSDVLGHFSEEDFSKTLGGYVKDTGVVLELY 690
            WL  +EEIFL+  TCA+AFRILR +GYDVSSDV   F+E+ F  +LGGY+KDT  VLELY
Sbjct: 261  WLQGNEEIFLDCITCAMAFRILRVNGYDVSSDVFTQFTEDHFFDSLGGYLKDTRTVLELY 320

Query: 689  RASHFAFPDEIFLEEQKMWSGQYLKRELSKSSIHADRQYECIRKEVDVALSYPYYANLQR 510
            RAS   +PDE  LE+Q  W+  +L++ LS  S +AD   ECI + V  AL+ PYYA+L+R
Sbjct: 321  RASQILYPDEPLLEKQNSWTNHFLEKCLSSGSSYADGPRECITEVVHNALNCPYYADLER 380

Query: 509  LENRRRAEHYDVDNLRILKSSYRPTNVDKKVILELAVEDFNLCQSIQQKELKQLERWVVE 330
            L NRR  E+Y+VD  RILK+SYR  N   +  L+LAVEDFNLCQ I Q+EL+QL RWVVE
Sbjct: 381  LTNRRSIENYNVDETRILKASYRCLNTGNQHFLKLAVEDFNLCQLIHQEELQQLGRWVVE 440

Query: 329  NRLDKLTFARQKQAYCYFSAAATLFPPELSDARMSWAKNSVLTTVVDDFYDVGGSREELL 150
             RL+KL FARQK  YCYFSAAATLF PELSDAR+SWAKN VLTTVVDDF+DVGGS EEL+
Sbjct: 441  KRLNKLKFARQKLGYCYFSAAATLFAPELSDARLSWAKNGVLTTVVDDFFDVGGSVEELI 500

Query: 149  NLIELVEKWDGVSATDICSENVEIIFNALRNTTNELGEMAIKHQHRNVT 3
            NLI+L+EKWD   +T  CSE VEIIF+ALR+T +E+G+ A   Q R VT
Sbjct: 501  NLIQLIEKWDVDESTHFCSEQVEIIFSALRSTISEIGDKAFTWQGRKVT 549



 Score = 84.0 bits (206), Expect(2) = e-115
 Identities = 37/73 (50%), Positives = 54/73 (73%)
 Frame = -2

Query: 1143 THLQDINCLNYLHSLLDVFGNSVPTAYPLEIYTQLCLIDDLESLGISRHFKKEIDSVLDK 964
            +HL++ +CL YL S+L  +GN+VPT YPL++Y++L ++D LE LGI RHF+KEI  VL++
Sbjct: 197  SHLRNADCLQYLQSVLQKYGNAVPTIYPLDVYSRLLMVDILERLGIDRHFRKEIKLVLEE 256

Query: 963  TYSCWCKVATFIF 925
            TY  W +    IF
Sbjct: 257  TYRYWLQGNEEIF 269


>ref|XP_002311286.1| ent-kaurene synthase [Populus trichocarpa]
            gi|222851106|gb|EEE88653.1| ent-kaurene synthase [Populus
            trichocarpa]
          Length = 743

 Score =  333 bits (855), Expect(2) = e-108
 Identities = 170/288 (59%), Positives = 215/288 (74%)
 Frame = -3

Query: 869  WLMKDEEIFLETATCAIAFRILRAHGYDVSSDVLGHFSEEDFSKTLGGYVKDTGVVLELY 690
            WL  +EEIF + ATCA+AFRILR +GYDVS        E+ FS +LGGY+KD+G  LELY
Sbjct: 273  WLQGEEEIFSDNATCALAFRILRLNGYDVSL-------EDHFSNSLGGYLKDSGAALELY 325

Query: 689  RASHFAFPDEIFLEEQKMWSGQYLKRELSKSSIHADRQYECIRKEVDVALSYPYYANLQR 510
            RA   ++PDE  LE+Q   +  +LK+ LS  S+  DR  + I  EV  AL++  +ANLQR
Sbjct: 326  RALQLSYPDESLLEKQNSRTSYFLKQGLSNVSLCGDRLRKNIIGEVHDALNFSDHANLQR 385

Query: 509  LENRRRAEHYDVDNLRILKSSYRPTNVDKKVILELAVEDFNLCQSIQQKELKQLERWVVE 330
            L  RRR +HY  D+ RILK+SYR + +  +  L+LAVEDFN+CQSIQ++E K +ERWVVE
Sbjct: 386  LAIRRRIKHYATDDTRILKTSYRCSTIGNQDFLKLAVEDFNICQSIQREEFKHIERWVVE 445

Query: 329  NRLDKLTFARQKQAYCYFSAAATLFPPELSDARMSWAKNSVLTTVVDDFYDVGGSREELL 150
             RLDKL FARQK+AYCYFSAAATLF PELSDARMSWAKN VLTTVVDDF+DVGGS EEL+
Sbjct: 446  RRLDKLKFARQKEAYCYFSAAATLFAPELSDARMSWAKNGVLTTVVDDFFDVGGSEEELV 505

Query: 149  NLIELVEKWDGVSATDICSENVEIIFNALRNTTNELGEMAIKHQHRNV 6
            NLIEL+E+WD   + D CSE VEII++A+ +T +E+G+ +   Q R+V
Sbjct: 506  NLIELIERWDVNGSADFCSEEVEIIYSAIHSTISEIGDKSFGWQGRDV 553



 Score = 87.4 bits (215), Expect(2) = e-108
 Identities = 40/72 (55%), Positives = 51/72 (70%)
 Frame = -2

Query: 1140 HLQDINCLNYLHSLLDVFGNSVPTAYPLEIYTQLCLIDDLESLGISRHFKKEIDSVLDKT 961
            H+QD  CL+Y+ SLL  FGN+VPT YPL+IY +L ++D LE LGI RHF+KE   VLD+T
Sbjct: 210  HIQDAECLHYIRSLLQKFGNAVPTIYPLDIYARLSMVDALERLGIDRHFRKERKFVLDET 269

Query: 960  YSCWCKVATFIF 925
            Y  W +    IF
Sbjct: 270  YRFWLQGEEEIF 281


>gb|AFG18184.1| ent-kaurene synthase [Malus x domestica]
          Length = 737

 Score =  333 bits (853), Expect(2) = e-107
 Identities = 173/290 (59%), Positives = 209/290 (72%), Gaps = 1/290 (0%)
 Frame = -3

Query: 869  WLMKDEEIFLETATCAIAFRILRAHGYDVSSDVLGHFSEEDFSKTLGGYVKDTGVVLELY 690
            WL  DE+I  + ATCA+AFR+LR +GYDVS+D L  +SE+    +LGGY+KD    LEL+
Sbjct: 260  WLQGDEDILSDAATCAMAFRLLRVNGYDVSADPLTQYSEDRLFNSLGGYMKDIDDALELF 319

Query: 689  RASH-FAFPDEIFLEEQKMWSGQYLKRELSKSSIHADRQYECIRKEVDVALSYPYYANLQ 513
            RAS     PDE  LE+Q  W+  +LK+ELS + I      + I  EVD AL  P YA L 
Sbjct: 320  RASETIIHPDESVLEKQHHWTSHFLKQELSNTLIRGHNLNKHIGLEVDDALKLPRYAILG 379

Query: 512  RLENRRRAEHYDVDNLRILKSSYRPTNVDKKVILELAVEDFNLCQSIQQKELKQLERWVV 333
            RL  R   ++Y+ D+ RILKS YR  N+  +  L+LAV+DFN CQSI ++EL  L RWV 
Sbjct: 380  RLSTRTAIKYYNTDSTRILKSYYRCLNIGNEDFLKLAVDDFNNCQSIHREELNHLTRWVE 439

Query: 332  ENRLDKLTFARQKQAYCYFSAAATLFPPELSDARMSWAKNSVLTTVVDDFYDVGGSREEL 153
            E RLDKL FARQKQAYCYFSAAATLFPPELSDAR+SWAKN VLTTVVDDF+DVGGS EEL
Sbjct: 440  EYRLDKLNFARQKQAYCYFSAAATLFPPELSDARISWAKNGVLTTVVDDFFDVGGSEEEL 499

Query: 152  LNLIELVEKWDGVSATDICSENVEIIFNALRNTTNELGEMAIKHQHRNVT 3
            +NLI+LVEKWD   + D CSENVEIIF+AL+NT NE+G  A   Q R+VT
Sbjct: 500  INLIQLVEKWDVNVSVDCCSENVEIIFSALKNTINEIGARAFTRQGRSVT 549



 Score = 83.6 bits (205), Expect(2) = e-107
 Identities = 37/64 (57%), Positives = 49/64 (76%)
 Frame = -2

Query: 1140 HLQDINCLNYLHSLLDVFGNSVPTAYPLEIYTQLCLIDDLESLGISRHFKKEIDSVLDKT 961
            HL++ +CL YL SLL+ FGN+VPT YPL+ Y +L +   LE+LGI RHF++EI SVLD+T
Sbjct: 197  HLKNADCLKYLRSLLEKFGNAVPTVYPLDKYARLSMAASLENLGIDRHFREEIRSVLDET 256

Query: 960  YSCW 949
            Y  W
Sbjct: 257  YRSW 260


>gb|AEN74904.1| ent-kaurene synthase [Pyrus communis]
          Length = 737

 Score =  331 bits (848), Expect(2) = e-107
 Identities = 172/290 (59%), Positives = 208/290 (71%), Gaps = 1/290 (0%)
 Frame = -3

Query: 869  WLMKDEEIFLETATCAIAFRILRAHGYDVSSDVLGHFSEEDFSKTLGGYVKDTGVVLELY 690
            WL  DE+I  + ATCA+AFR+LR +GYDVS+D L  +SE+    +LGGY+KD    LEL+
Sbjct: 260  WLQGDEDILSDAATCAMAFRLLRVNGYDVSADPLTQYSEDRLFNSLGGYMKDIDDALELF 319

Query: 689  RASH-FAFPDEIFLEEQKMWSGQYLKRELSKSSIHADRQYECIRKEVDVALSYPYYANLQ 513
            RAS     PDE  LE+Q  W+  +LK+ELS + I   +  + I  EVD AL  P YA L 
Sbjct: 320  RASETIIHPDESVLEKQHHWTSHFLKQELSNTLIRGHKLNKHIGLEVDDALKLPRYAILG 379

Query: 512  RLENRRRAEHYDVDNLRILKSSYRPTNVDKKVILELAVEDFNLCQSIQQKELKQLERWVV 333
            RL  R   ++Y+ D+ RILKS YR  N+  +  L+LAV+D N CQSI ++EL  L RWV 
Sbjct: 380  RLSTRTAIKYYNTDSTRILKSYYRCLNIGNEDFLKLAVDDLNNCQSIHREELNHLTRWVE 439

Query: 332  ENRLDKLTFARQKQAYCYFSAAATLFPPELSDARMSWAKNSVLTTVVDDFYDVGGSREEL 153
            E RLDKL FARQKQAYCYFSAAATLFPPELSDAR+SWAKN VLTTVVDDF+DVGGS EEL
Sbjct: 440  EYRLDKLNFARQKQAYCYFSAAATLFPPELSDARISWAKNGVLTTVVDDFFDVGGSEEEL 499

Query: 152  LNLIELVEKWDGVSATDICSENVEIIFNALRNTTNELGEMAIKHQHRNVT 3
            +NLI+LVEKWD     D CSENVEIIF+AL+NT NE+G  A   Q R+VT
Sbjct: 500  INLIQLVEKWDVNVGVDCCSENVEIIFSALKNTINEIGARAFTRQGRSVT 549



 Score = 84.7 bits (208), Expect(2) = e-107
 Identities = 38/65 (58%), Positives = 50/65 (76%)
 Frame = -2

Query: 1143 THLQDINCLNYLHSLLDVFGNSVPTAYPLEIYTQLCLIDDLESLGISRHFKKEIDSVLDK 964
            THL++ +CL YL SLL+ FGN+VPT YPL+ Y +L +   LE+LGI RHF++EI SVLD+
Sbjct: 196  THLKNADCLKYLRSLLEKFGNAVPTVYPLDKYARLSMAASLENLGIDRHFREEIRSVLDE 255

Query: 963  TYSCW 949
            TY  W
Sbjct: 256  TYRGW 260


>ref|XP_003532911.1| PREDICTED: ent-kaur-16-ene synthase, chloroplastic-like [Glycine max]
          Length = 802

 Score =  329 bits (843), Expect(2) = e-105
 Identities = 166/289 (57%), Positives = 212/289 (73%), Gaps = 1/289 (0%)
 Frame = -3

Query: 869  WLMKDEEIFLETATCAIAFRILRAHGYDVSSDVLGHFSEEDFSKTLGGYVKDTGVVLELY 690
            W+   E+IFL+  TCA+AFR+LR +GYDVSSD    +SE+ F+++L GY+KD G V+ELY
Sbjct: 305  WMQGVEDIFLDPTTCAMAFRMLRLNGYDVSSDPFYQYSEDKFAESLKGYLKDVGAVIELY 364

Query: 689  RASH-FAFPDEIFLEEQKMWSGQYLKRELSKSSIHADRQYECIRKEVDVALSYPYYANLQ 513
            RAS     PDE  L  Q +W+   LK+E S   ++AD+    +  E+   L++PY+ANL+
Sbjct: 365  RASQAIIHPDESILVRQSLWTKHLLKQESSPYRLYADKLRSYVDLEIKDVLNFPYHANLE 424

Query: 512  RLENRRRAEHYDVDNLRILKSSYRPTNVDKKVILELAVEDFNLCQSIQQKELKQLERWVV 333
            RL NRR  EHY+    RILK+SYR  N+  + IL+LAVEDFN+CQ+I  +ELKQL RWVV
Sbjct: 425  RLLNRRSMEHYNTVETRILKASYRSCNLANQEILKLAVEDFNICQAIHIEELKQLSRWVV 484

Query: 332  ENRLDKLTFARQKQAYCYFSAAATLFPPELSDARMSWAKNSVLTTVVDDFYDVGGSREEL 153
            E RLD L FARQK AYCYFS AAT+F PELSDAR+SWAK+ VLTTVVDDF+DVGGS EE 
Sbjct: 485  ERRLDTLKFARQKLAYCYFSCAATIFSPELSDARISWAKSGVLTTVVDDFFDVGGSEEEH 544

Query: 152  LNLIELVEKWDGVSATDICSENVEIIFNALRNTTNELGEMAIKHQHRNV 6
            +NLI+LVEKWD    T  CSE V+IIF+A+ +T  E+GE ++K Q RNV
Sbjct: 545  VNLIQLVEKWDVDINTVCCSETVKIIFSAIHSTVCEIGEKSVKQQGRNV 593



 Score = 81.6 bits (200), Expect(2) = e-105
 Identities = 37/70 (52%), Positives = 50/70 (71%)
 Frame = -2

Query: 1134 QDINCLNYLHSLLDVFGNSVPTAYPLEIYTQLCLIDDLESLGISRHFKKEIDSVLDKTYS 955
            ++  CL YL S+L+ F N+VPT YPL+IY +LC+ID LE LGI+ HFK+EI SVLD+ + 
Sbjct: 244  KNAECLGYLQSVLEKFENAVPTTYPLDIYARLCMIDSLERLGINHHFKEEIRSVLDEIFR 303

Query: 954  CWCKVATFIF 925
             W +    IF
Sbjct: 304  YWMQGVEDIF 313