BLASTX nr result
ID: Aconitum21_contig00003573
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Aconitum21_contig00003573
(1593 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002268942.2| PREDICTED: 2-isopropylmalate synthase 2, chl... 592 e-166
ref|XP_004165080.1| PREDICTED: 2-isopropylmalate synthase 2, chl... 590 e-166
ref|NP_177544.1| 2-isopropylmalate synthase 2 [Arabidopsis thali... 586 e-165
gb|ABA26446.1| 2-isopropylmalate synthase 1 [Brassica insularis] 583 e-164
gb|AAW81741.1| Putative 2-isopropylmalate synthase [Brassica ole... 583 e-164
>ref|XP_002268942.2| PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Vitis
vinifera]
Length = 628
Score = 592 bits (1525), Expect = e-166
Identities = 301/429 (70%), Positives = 350/429 (81%), Gaps = 8/429 (1%)
Frame = -3
Query: 1552 LFRLSSSHSLT--------PTTMNPRPAYTPNLIPDSNYIRILDTTLRDGEQSPGASMTI 1397
LF SHSL P RP Y PN I D NY+RI DTTLRDGEQSPGA+MT
Sbjct: 47 LFFKPPSHSLATKVLSHRLPVRSALRPEYIPNRIDDPNYVRIFDTTLRDGEQSPGATMTS 106
Query: 1396 QEKLDIARQLYKLGVDVIEAGFPCSSKDNFEAVKLIAQEVGNRDSGDNGYVPVISGVARA 1217
+EKLDIARQL KLGVD+IEAGFP +SKD+ +AVK+IA+EVGN +NG+VPVI G+AR
Sbjct: 107 KEKLDIARQLAKLGVDIIEAGFPAASKDDLDAVKIIAKEVGNAVD-ENGHVPVICGLARC 165
Query: 1216 NKADIDAAWEAVKYAKRPRISTFIATSDIHLKHKLKKTREEVISIAKEMVSYARSLGCND 1037
N+ DI AWEAVKYAKRPRI TFIATS+IH+K+KLKK+ +EVI IA+EMV YARSLGC+D
Sbjct: 166 NRNDISTAWEAVKYAKRPRIHTFIATSEIHMKYKLKKSEKEVIEIAREMVKYARSLGCDD 225
Query: 1036 VQFNPEDATRSDREYLYHILGEVIKAGATTINVCDTVGYMFPSEFGKLVADIKLNTPGIE 857
V+F+PEDA RS+RE+LY ILGEVIKAGATT+N+ DTVGY P+EFG+L+ADIK NTPGIE
Sbjct: 226 VEFSPEDAGRSEREFLYKILGEVIKAGATTLNIPDTVGYTVPNEFGQLIADIKSNTPGIE 285
Query: 856 NVVIATHCHNDXXXXXXXXXXXAYEGARQAEVTINGIGERAGNASLEEVVMAIRCRGGEL 677
NV+I+THC ND A GARQ EVTINGIGERAGNASLEEVVMA++CRG +
Sbjct: 286 NVIISTHCQNDLGLSTANTIAGACAGARQVEVTINGIGERAGNASLEEVVMALKCRGEKA 345
Query: 676 MEGIHTGINAKHIVKISKMVEEYTGMNVQPHKAIVGANAFSHESGIHQDGMLKFRGTYEI 497
+ G++TGIN +HIVK SKMV EY+G+ VQPHKAIVGANAF+HESGIHQDGMLK + TYEI
Sbjct: 346 LGGLYTGINIRHIVKTSKMVAEYSGLQVQPHKAIVGANAFAHESGIHQDGMLKHKETYEI 405
Query: 496 ISPEDIGLSRSNGSGIVLGKLSGRHALKSRLLELGYELDARELDAVFWRFKEIAEKKKHL 317
ISPEDIGL RSN SGIVLGKLSGRHALKSRLLELGYE++ ELD +FWRFKE+AEKKK+L
Sbjct: 406 ISPEDIGLFRSNESGIVLGKLSGRHALKSRLLELGYEIEGMELDDIFWRFKEVAEKKKNL 465
Query: 316 CDGDIKALI 290
D D+ AL+
Sbjct: 466 TDVDVIALV 474
>ref|XP_004165080.1| PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Cucumis
sativus]
Length = 639
Score = 590 bits (1522), Expect = e-166
Identities = 296/433 (68%), Positives = 352/433 (81%)
Frame = -3
Query: 1519 PTTMNPRPAYTPNLIPDSNYIRILDTTLRDGEQSPGASMTIQEKLDIARQLYKLGVDVIE 1340
P + RP Y PN IPD +Y+R+ DTTLRDGEQSPGAS+T++EKLDIARQL KLGVD+IE
Sbjct: 73 PKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIARQLAKLGVDIIE 132
Query: 1339 AGFPCSSKDNFEAVKLIAQEVGNRDSGDNGYVPVISGVARANKADIDAAWEAVKYAKRPR 1160
AGFP +SK++FEAVK+IA+E+GN D GYVPVI G++R N+ DI AWEAVKYAKRPR
Sbjct: 133 AGFPAASKEDFEAVKMIAKEIGNAVDED-GYVPVICGLSRCNEKDIRTAWEAVKYAKRPR 191
Query: 1159 ISTFIATSDIHLKHKLKKTREEVISIAKEMVSYARSLGCNDVQFNPEDATRSDREYLYHI 980
I TFIATS+IH++HKL+KT+EEVI IA+ MV +ARSLGC+DV+F+PEDA RSDRE+LY I
Sbjct: 192 IHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDAGRSDREFLYQI 251
Query: 979 LGEVIKAGATTINVCDTVGYMFPSEFGKLVADIKLNTPGIENVVIATHCHNDXXXXXXXX 800
LGEVIKAGATT+N+ DTVGY P EFGKL+ADIK NTPGIENV+I++HC ND
Sbjct: 252 LGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHCQNDLGLATANT 311
Query: 799 XXXAYEGARQAEVTINGIGERAGNASLEEVVMAIRCRGGELMEGIHTGINAKHIVKISKM 620
A GARQ EVTINGIGERAGNASLEEVVMA++CRG ++ G+HTGIN++HI SKM
Sbjct: 312 VAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGINSRHIFLTSKM 371
Query: 619 VEEYTGMNVQPHKAIVGANAFSHESGIHQDGMLKFRGTYEIISPEDIGLSRSNGSGIVLG 440
VEE+TG+NVQPHKAIVGANAF+H SGIHQDGMLK +GTYEI++PEDIG RSN +GIVLG
Sbjct: 372 VEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGYERSNDAGIVLG 431
Query: 439 KLSGRHALKSRLLELGYELDARELDAVFWRFKEIAEKKKHLCDGDIKALILHKDENLKAV 260
KLSGRHALKS LLELGYELD LD VFWRFK +AE+KK + D D++AL+ DE +
Sbjct: 432 KLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRALV--SDEVFQPT 489
Query: 259 VV*FLAGGQVING 221
V+ L QV G
Sbjct: 490 VLWKLLDMQVTCG 502
>ref|NP_177544.1| 2-isopropylmalate synthase 2 [Arabidopsis thaliana]
gi|75168891|sp|Q9C550.1|LEU12_ARATH RecName:
Full=2-isopropylmalate synthase 2, chloroplastic;
AltName: Full=2-isopropylmalate synthase 1; AltName:
Full=Methylthioalkylmalate synthase-like 3; Flags:
Precursor gi|12325154|gb|AAG52530.1|AC016662_24 putative
2-isopropylmalate synthase; 30920-27612 [Arabidopsis
thaliana] gi|12330687|gb|AAG52882.1|AF327647_1
2-isopropylmalate synthase [Arabidopsis thaliana]
gi|16323111|gb|AAL15290.1| At1g74040/F2P9_9 [Arabidopsis
thaliana] gi|23463041|gb|AAN33190.1| At1g74040/F2P9_9
[Arabidopsis thaliana] gi|332197419|gb|AEE35540.1|
2-isopropylmalate synthase 2 [Arabidopsis thaliana]
Length = 631
Score = 586 bits (1511), Expect = e-165
Identities = 294/420 (70%), Positives = 342/420 (81%)
Frame = -3
Query: 1549 FRLSSSHSLTPTTMNPRPAYTPNLIPDSNYIRILDTTLRDGEQSPGASMTIQEKLDIARQ 1370
+ LS+S L P RP Y PN I D NY+RI DTTLRDGEQSPGA++T +EKLDIARQ
Sbjct: 57 YSLSASSPLPPHAPRRRPNYIPNRISDPNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQ 116
Query: 1369 LYKLGVDVIEAGFPCSSKDNFEAVKLIAQEVGNRDSGDNGYVPVISGVARANKADIDAAW 1190
L KLGVD+IEAGFP +SKD+FEAVK IA+ VGN +NGYVPVI G++R NK DI+ AW
Sbjct: 117 LAKLGVDIIEAGFPAASKDDFEAVKTIAETVGNTVD-ENGYVPVICGLSRCNKKDIETAW 175
Query: 1189 EAVKYAKRPRISTFIATSDIHLKHKLKKTREEVISIAKEMVSYARSLGCNDVQFNPEDAT 1010
EAVKYAKRPRI TFIATSDIHLK+KLKK++EEVI IA+ MV +ARSLGC DV+F+PEDA
Sbjct: 176 EAVKYAKRPRIHTFIATSDIHLKYKLKKSKEEVIEIARNMVRFARSLGCEDVEFSPEDAG 235
Query: 1009 RSDREYLYHILGEVIKAGATTINVCDTVGYMFPSEFGKLVADIKLNTPGIENVVIATHCH 830
RS+REYLY ILGEVIKAGATT+N+ DTVG PSEFG+L+ADIK NTPGI+NV+I+THC
Sbjct: 236 RSEREYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQ 295
Query: 829 NDXXXXXXXXXXXAYEGARQAEVTINGIGERAGNASLEEVVMAIRCRGGELMEGIHTGIN 650
ND A+ GARQ EVTINGIGERAGNASLEEVVMAI+CRG ++ G+ TGI+
Sbjct: 296 NDLGLSTANTLSGAHSGARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGID 355
Query: 649 AKHIVKISKMVEEYTGMNVQPHKAIVGANAFSHESGIHQDGMLKFRGTYEIISPEDIGLS 470
+HIV SKMVEEYTGM QPHKAIVGANAF+HESGIHQDGMLK +GTYEI+SPE+IGL
Sbjct: 356 TRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIMSPEEIGLE 415
Query: 469 RSNGSGIVLGKLSGRHALKSRLLELGYELDARELDAVFWRFKEIAEKKKHLCDGDIKALI 290
RSN +GIVLGKLSGRHALK RL ELGY LD +L +FWRFK +AE+KK + D D+ AL+
Sbjct: 416 RSNDAGIVLGKLSGRHALKDRLNELGYVLDDGQLSNLFWRFKAVAEQKKRVTDADLIALV 475
>gb|ABA26446.1| 2-isopropylmalate synthase 1 [Brassica insularis]
Length = 623
Score = 583 bits (1504), Expect = e-164
Identities = 294/420 (70%), Positives = 342/420 (81%), Gaps = 1/420 (0%)
Frame = -3
Query: 1546 RLSSSHS-LTPTTMNPRPAYTPNLIPDSNYIRILDTTLRDGEQSPGASMTIQEKLDIARQ 1370
RLS S S +P RP Y PN I D NY+R+ DTTLRDGEQSPGA++T +EKLDIARQ
Sbjct: 51 RLSCSLSDPSPPLCRRRPEYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQ 110
Query: 1369 LYKLGVDVIEAGFPCSSKDNFEAVKLIAQEVGNRDSGDNGYVPVISGVARANKADIDAAW 1190
L KLGVDVIEAGFP +SKD+FEAVK IA VGN GD GYVPVI G++R NK DI+ AW
Sbjct: 111 LAKLGVDVIEAGFPAASKDDFEAVKTIAGTVGNAVDGD-GYVPVICGLSRCNKRDIETAW 169
Query: 1189 EAVKYAKRPRISTFIATSDIHLKHKLKKTREEVISIAKEMVSYARSLGCNDVQFNPEDAT 1010
EAVKYAKRPRI TFIATSDIHL++KLKK+++EVI IA+ MV +ARSLGC DV+F+PEDA
Sbjct: 170 EAVKYAKRPRIHTFIATSDIHLEYKLKKSKDEVIEIARNMVKFARSLGCEDVEFSPEDAG 229
Query: 1009 RSDREYLYHILGEVIKAGATTINVCDTVGYMFPSEFGKLVADIKLNTPGIENVVIATHCH 830
RS+RE+LY ILGEVIKAGATT+N+ DTVG PSEFG+L+ADIK NTPGIENV+I+THC
Sbjct: 230 RSEREFLYQILGEVIKAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIENVIISTHCQ 289
Query: 829 NDXXXXXXXXXXXAYEGARQAEVTINGIGERAGNASLEEVVMAIRCRGGELMEGIHTGIN 650
ND A+ GARQ EVTINGIGERAGNASLEEVVMAI+CRG ++ G++TGI+
Sbjct: 290 NDLGLSTANTLSGAHSGARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLYTGID 349
Query: 649 AKHIVKISKMVEEYTGMNVQPHKAIVGANAFSHESGIHQDGMLKFRGTYEIISPEDIGLS 470
+HIV SKMVE+YTGM QPHKAIVGANAF+HESGIHQDGMLK +GTYEII PE+IGL
Sbjct: 350 TRHIVMTSKMVEDYTGMQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLE 409
Query: 469 RSNGSGIVLGKLSGRHALKSRLLELGYELDARELDAVFWRFKEIAEKKKHLCDGDIKALI 290
RSN +GIVLGKLSGRHALK RL ELGY LD +L ++FWRFK +AE+KK + D DI AL+
Sbjct: 410 RSNDAGIVLGKLSGRHALKDRLTELGYVLDDEQLSSIFWRFKSVAERKKRVTDADIIALV 469
>gb|AAW81741.1| Putative 2-isopropylmalate synthase [Brassica oleracea]
Length = 623
Score = 583 bits (1503), Expect = e-164
Identities = 293/420 (69%), Positives = 342/420 (81%), Gaps = 1/420 (0%)
Frame = -3
Query: 1546 RLSSSHS-LTPTTMNPRPAYTPNLIPDSNYIRILDTTLRDGEQSPGASMTIQEKLDIARQ 1370
RLS S S +P RP Y PN I D NY+R+ DTTLRDGEQSPGA++T +EKLDIARQ
Sbjct: 51 RLSCSLSDPSPPLRRCRPEYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQ 110
Query: 1369 LYKLGVDVIEAGFPCSSKDNFEAVKLIAQEVGNRDSGDNGYVPVISGVARANKADIDAAW 1190
L KLGVDVIEAGFP +SKD+FEAVK IA+ VGN GD GYVPVI G++R NK DI+ AW
Sbjct: 111 LAKLGVDVIEAGFPAASKDDFEAVKTIAETVGNAVDGD-GYVPVICGLSRCNKRDIETAW 169
Query: 1189 EAVKYAKRPRISTFIATSDIHLKHKLKKTREEVISIAKEMVSYARSLGCNDVQFNPEDAT 1010
EAVKYAKRPRI TFIATSDIHL++KLKK+++EVI A+ MV +ARSLGC DV+F+PEDA
Sbjct: 170 EAVKYAKRPRIHTFIATSDIHLEYKLKKSKDEVIEFARNMVKFARSLGCEDVEFSPEDAG 229
Query: 1009 RSDREYLYHILGEVIKAGATTINVCDTVGYMFPSEFGKLVADIKLNTPGIENVVIATHCH 830
RS+RE+LY ILGEVIKAGATT+N+ DTVG PSEFG+L+ADIK NTPGIENV+I+THC
Sbjct: 230 RSEREFLYQILGEVIKAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIENVIISTHCQ 289
Query: 829 NDXXXXXXXXXXXAYEGARQAEVTINGIGERAGNASLEEVVMAIRCRGGELMEGIHTGIN 650
ND A+ GARQ EVTINGIGERAGNASLEEVVMAI+CRG ++ G++TGI+
Sbjct: 290 NDLGLSTANTLSGAHSGARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLYTGID 349
Query: 649 AKHIVKISKMVEEYTGMNVQPHKAIVGANAFSHESGIHQDGMLKFRGTYEIISPEDIGLS 470
+HIV SKMVE+YTGM QPHKAIVGANAF+HESGIHQDGMLK +GTYEII PE+IGL
Sbjct: 350 TRHIVMTSKMVEDYTGMQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLE 409
Query: 469 RSNGSGIVLGKLSGRHALKSRLLELGYELDARELDAVFWRFKEIAEKKKHLCDGDIKALI 290
RSN +GIVLGKLSGRHALK RL ELGY LD +L ++FWRFK +AE+KK + D DI AL+
Sbjct: 410 RSNDAGIVLGKLSGRHALKDRLTELGYVLDDEQLSSIFWRFKSVAERKKRVTDADIIALV 469