BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Aconitum21_contig00003271
         (1333 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAQ63464.1| ent-kaurene oxidase CYP701A5 [Stevia rebaudiana] ...   314   4e-83
gb|AAY42951.1| kaurene oxidase [Stevia rebaudiana]                    311   2e-82
dbj|BAG71198.1| ent-kaurene oxidase 2 [Lactuca sativa]                311   3e-82
gb|AEF32084.1| ent-kaurene oxidase [Castanea mollissima]              310   7e-82
ref|XP_002510288.1| ent-kaurene oxidase, putative [Ricinus commu...   308   3e-81

>gb|AAQ63464.1| ent-kaurene oxidase CYP701A5 [Stevia rebaudiana]
            gi|76446107|gb|ABA42921.1| kaurene oxidase [Stevia
            rebaudiana]
          Length = 513

 Score =  314 bits (804), Expect = 4e-83
 Identities = 155/259 (59%), Positives = 204/259 (78%), Gaps = 1/259 (0%)
 Frame = -3

Query: 1331 SDYGEFHKMAKRNIITGLLGGNAQKRYRGHRVTMMNNIIHKLHTYSKEYP-LEAINLRVV 1155
            SDY ++HK  KR+I+T +LG NAQK++R HR  MM+NI  +LH + K  P  E ++LR +
Sbjct: 136  SDYDDYHKTVKRHILTAVLGPNAQKKHRIHRDIMMDNISTQLHEFVKNNPEQEEVDLRKI 195

Query: 1154 FQTELFGLALKQAVGKDLESPLYVEELGTALSKNELYDALVVEPMMGAIDVDWRDFFPYL 975
            FQ+ELFGLA++QA+GKD+ES LYVE+L   ++++E++  LVV+PMMGAIDVDWRDFFPYL
Sbjct: 196  FQSELFGLAMRQALGKDVES-LYVEDLKITMNRDEIFQVLVVDPMMGAIDVDWRDFFPYL 254

Query: 974  RWIPNKRLETRINHMAKCRKASMKALIGMHKNRIESGEGLDSFLDFLLASEGKNYTEDVL 795
            +W+PNK+ E  I  M   R+A MK+LI  HK RI SGE L+S++D+LL SE +  T+  L
Sbjct: 255  KWVPNKKFENTIQQMYIRREAVMKSLIKEHKKRIASGEKLNSYIDYLL-SEAQTLTDQQL 313

Query: 794  FMLVWESIIEMTDTILVTTEWAMYELAKNKSCQDYLFREIESVCGSNKISEDHLSQLPYL 615
             M +WE IIE +DT +VTTEWAMYELAKN   QD L+R+I+SVCGS KI+E+HLSQLPY+
Sbjct: 314  LMSLWEPIIESSDTTMVTTEWAMYELAKNPKLQDRLYRDIKSVCGSEKITEEHLSQLPYI 373

Query: 614  SAIFHETLRRYSPVALIPL 558
            +AIFHETLRR+SPV +IPL
Sbjct: 374  TAIFHETLRRHSPVPIIPL 392



 Score = 79.3 bits (194), Expect = 2e-12
 Identities = 38/68 (55%), Positives = 47/68 (69%)
 Frame = -3

Query: 326 DMFKTMAFGAGKRVCSGSQQAMTLSCLAIGAFVQEFEWRLSEGQKEEFNTLGLTSYKLHA 147
           D  KTMAFG GKRVC+GS QA+  + + IG  VQEFEW+L +  +EE NT+GLT+  L  
Sbjct: 445 DFQKTMAFGGGKRVCAGSLQALLTASIGIGRMVQEFEWKLKDMTQEEVNTIGLTTQMLRP 504

Query: 146 LMAFITPR 123
           L A I PR
Sbjct: 505 LRAIIKPR 512


>gb|AAY42951.1| kaurene oxidase [Stevia rebaudiana]
          Length = 513

 Score =  311 bits (797), Expect = 2e-82
 Identities = 154/259 (59%), Positives = 204/259 (78%), Gaps = 1/259 (0%)
 Frame = -3

Query: 1331 SDYGEFHKMAKRNIITGLLGGNAQKRYRGHRVTMMNNIIHKLHTYSKEYP-LEAINLRVV 1155
            SDY ++HK  KR+I+T +LG NAQK++R HR  MM+NI  +LH + K  P  E ++LR +
Sbjct: 136  SDYDDYHKTVKRHILTAVLGPNAQKKHRIHRDIMMDNISTQLHEFVKNNPEQEEVDLRKI 195

Query: 1154 FQTELFGLALKQAVGKDLESPLYVEELGTALSKNELYDALVVEPMMGAIDVDWRDFFPYL 975
            FQ+ELFGLA++QA+GKD+ES LYVE+L   ++++E++  LVV+PMMGAIDVDWRDFFPYL
Sbjct: 196  FQSELFGLAMRQALGKDVES-LYVEDLKITMNRDEIFQVLVVDPMMGAIDVDWRDFFPYL 254

Query: 974  RWIPNKRLETRINHMAKCRKASMKALIGMHKNRIESGEGLDSFLDFLLASEGKNYTEDVL 795
            +W+PNK+ E  I  M   R+A MK+LI  +K RI SGE L+S++D+LL SE +  T+  L
Sbjct: 255  KWVPNKKFENTIQQMYIRREAVMKSLIKENKKRIASGEKLNSYIDYLL-SEAQTLTDQQL 313

Query: 794  FMLVWESIIEMTDTILVTTEWAMYELAKNKSCQDYLFREIESVCGSNKISEDHLSQLPYL 615
             M +WE IIE +DT +VTTEWAMYELAKN   QD L+R+I+SVCGS KI+E+HLSQLPY+
Sbjct: 314  LMSLWEPIIESSDTTMVTTEWAMYELAKNPKLQDRLYRDIKSVCGSEKITEEHLSQLPYI 373

Query: 614  SAIFHETLRRYSPVALIPL 558
            +AIFHETLRR+SPV +IPL
Sbjct: 374  TAIFHETLRRHSPVPIIPL 392



 Score = 79.3 bits (194), Expect = 2e-12
 Identities = 38/68 (55%), Positives = 47/68 (69%)
 Frame = -3

Query: 326 DMFKTMAFGAGKRVCSGSQQAMTLSCLAIGAFVQEFEWRLSEGQKEEFNTLGLTSYKLHA 147
           D  KTMAFG GKRVC+GS QA+  + + IG  VQEFEW+L +  +EE NT+GLT+  L  
Sbjct: 445 DFQKTMAFGGGKRVCAGSLQALLTASIGIGRMVQEFEWKLKDMTQEEVNTIGLTTQMLRP 504

Query: 146 LMAFITPR 123
           L A I PR
Sbjct: 505 LRAIIKPR 512


>dbj|BAG71198.1| ent-kaurene oxidase 2 [Lactuca sativa]
          Length = 512

 Score =  311 bits (796), Expect = 3e-82
 Identities = 148/258 (57%), Positives = 203/258 (78%)
 Frame = -3

Query: 1331 SDYGEFHKMAKRNIITGLLGGNAQKRYRGHRVTMMNNIIHKLHTYSKEYPLEAINLRVVF 1152
            SDY ++HK  KR+I+T +LG NAQK++R HR TMM N+ ++LH + ++ P + +NLR +F
Sbjct: 136  SDYHDYHKTVKRHILTAVLGPNAQKKFRAHRDTMMENVSNELHAFFEKNPNQEVNLRKIF 195

Query: 1151 QTELFGLALKQAVGKDLESPLYVEELGTALSKNELYDALVVEPMMGAIDVDWRDFFPYLR 972
            Q++LFGLA+KQA+GKD+ES +YV++L T + + E+++ LVV+PMMGAI+VDWRDFFPYL+
Sbjct: 196  QSQLFGLAMKQALGKDVES-IYVKDLETTMKREEIFEVLVVDPMMGAIEVDWRDFFPYLK 254

Query: 971  WIPNKRLETRINHMAKCRKASMKALIGMHKNRIESGEGLDSFLDFLLASEGKNYTEDVLF 792
            W+PNK  E  I+ M   R+A MKALI  HK RI SGE L+S++D+LL SE +  T+  L 
Sbjct: 255  WVPNKSFENIIHRMYTRREAVMKALIQEHKKRIASGENLNSYIDYLL-SEAQTLTDKQLL 313

Query: 791  MLVWESIIEMTDTILVTTEWAMYELAKNKSCQDYLFREIESVCGSNKISEDHLSQLPYLS 612
            M +WE IIE +DT +VTTEWAMYELAKN + QD L+ EI+SVCGS KI+E++LSQLPYL 
Sbjct: 314  MSLWEPIIESSDTTMVTTEWAMYELAKNPNMQDRLYEEIQSVCGSEKITEENLSQLPYLY 373

Query: 611  AIFHETLRRYSPVALIPL 558
            A+F ETLR++ PV ++PL
Sbjct: 374  AVFQETLRKHCPVPIMPL 391



 Score = 99.8 bits (247), Expect = 1e-18
 Identities = 46/79 (58%), Positives = 60/79 (75%)
 Frame = -3

Query: 356 RFLDSKFDPTDMFKTMAFGAGKRVCSGSQQAMTLSCLAIGAFVQEFEWRLSEGQKEEFNT 177
           RFL  K +  D++KTMAFG GKRVC+GS QAM +SC+ IG  VQ+FEW+L +  +E+ NT
Sbjct: 435 RFLSEK-ESMDLYKTMAFGGGKRVCAGSLQAMVISCIGIGRLVQDFEWKLKDDAEEDVNT 493

Query: 176 LGLTSYKLHALMAFITPRE 120
           LGLT+ KLH L+A I PR+
Sbjct: 494 LGLTTQKLHPLLALINPRK 512


>gb|AEF32084.1| ent-kaurene oxidase [Castanea mollissima]
          Length = 514

 Score =  310 bits (793), Expect = 7e-82
 Identities = 148/258 (57%), Positives = 201/258 (77%)
 Frame = -3

Query: 1331 SDYGEFHKMAKRNIITGLLGGNAQKRYRGHRVTMMNNIIHKLHTYSKEYPLEAINLRVVF 1152
            SDY EFHKM K+ I+  LLG NAQKR+R HR T++ N+++KLH ++K  PL+A+N R +F
Sbjct: 137  SDYNEFHKMVKKYILAELLGANAQKRHRIHRDTLIENVLNKLHAHTKNSPLQAVNFRKIF 196

Query: 1151 QTELFGLALKQAVGKDLESPLYVEELGTALSKNELYDALVVEPMMGAIDVDWRDFFPYLR 972
            ++ELFGLA+KQA+G D++S L+VEELGT LS+ E+Y+ LV + + GAI+VDWRDFFPYL+
Sbjct: 197  ESELFGLAMKQALGYDVDS-LFVEELGTTLSREEIYNVLVSDMLKGAIEVDWRDFFPYLK 255

Query: 971  WIPNKRLETRINHMAKCRKASMKALIGMHKNRIESGEGLDSFLDFLLASEGKNYTEDVLF 792
            WIPNK  E +I  +A  R+A M +++   K  I SG+G + +L++LL SE K  TE  + 
Sbjct: 256  WIPNKSFEMKIQRLASRRQAVMNSIVKEQKKSIASGKGENCYLNYLL-SEAKTLTEKQIS 314

Query: 791  MLVWESIIEMTDTILVTTEWAMYELAKNKSCQDYLFREIESVCGSNKISEDHLSQLPYLS 612
            +L WE+IIE  DT +VTTEWAMYELAKN   QD L+ EI++VCG++KI+E+HLS+LPYLS
Sbjct: 315  ILAWETIIETADTTVVTTEWAMYELAKNPKQQDRLYNEIQNVCGTDKITEEHLSKLPYLS 374

Query: 611  AIFHETLRRYSPVALIPL 558
            A+FHETLR+YSP  L+PL
Sbjct: 375  AVFHETLRKYSPSPLVPL 392



 Score =  106 bits (264), Expect = 2e-20
 Identities = 46/78 (58%), Positives = 62/78 (79%)
 Frame = -3

Query: 356 RFLDSKFDPTDMFKTMAFGAGKRVCSGSQQAMTLSCLAIGAFVQEFEWRLSEGQKEEFNT 177
           RFLD K+DP DM+KTM+FG+GKRVC+GS QA  ++C +IG  VQEFEWRL +G+ E  +T
Sbjct: 436 RFLDEKYDPMDMYKTMSFGSGKRVCAGSLQASLIACTSIGRLVQEFEWRLKDGEVENVDT 495

Query: 176 LGLTSYKLHALMAFITPR 123
           LGLT++KL+ + A + PR
Sbjct: 496 LGLTTHKLYPMQAILQPR 513


>ref|XP_002510288.1| ent-kaurene oxidase, putative [Ricinus communis]
            gi|223550989|gb|EEF52475.1| ent-kaurene oxidase, putative
            [Ricinus communis]
          Length = 513

 Score =  308 bits (788), Expect = 3e-81
 Identities = 145/258 (56%), Positives = 199/258 (77%)
 Frame = -3

Query: 1331 SDYGEFHKMAKRNIITGLLGGNAQKRYRGHRVTMMNNIIHKLHTYSKEYPLEAINLRVVF 1152
            SDY EFHKM KR ++T +LG NAQ+R+R HR T++ NI  + H + K  P + +N R +F
Sbjct: 136  SDYDEFHKMVKRYLLTNVLGANAQRRHRCHRDTLIKNISSRFHAHVKTNPEQTVNFRDIF 195

Query: 1151 QTELFGLALKQAVGKDLESPLYVEELGTALSKNELYDALVVEPMMGAIDVDWRDFFPYLR 972
            ++ELFGL+LK+A+GKD++S +YV+ELGT LS+ E+++ LV++PM GAIDVDWRDFFPYLR
Sbjct: 196  ESELFGLSLKEALGKDVQS-IYVDELGTTLSRKEIFEVLVLDPMEGAIDVDWRDFFPYLR 254

Query: 971  WIPNKRLETRINHMAKCRKASMKALIGMHKNRIESGEGLDSFLDFLLASEGKNYTEDVLF 792
            W+PNK  E +I  M   RKA M ALI   K RI  GE ++ +LDFL  S     T++ + 
Sbjct: 255  WVPNKSWEMKIQQMHFRRKAVMGALIKEQKKRIALGEEVNCYLDFL--SSESTLTDEQIR 312

Query: 791  MLVWESIIEMTDTILVTTEWAMYELAKNKSCQDYLFREIESVCGSNKISEDHLSQLPYLS 612
            ML+WE+I+E +DT +V TEWA+YELAKN +CQD L+R+I++VCGS KI+E+HLS+LPYL+
Sbjct: 313  MLIWETIVETSDTTMVATEWAIYELAKNPNCQDLLYRQIQNVCGSEKITEEHLSKLPYLN 372

Query: 611  AIFHETLRRYSPVALIPL 558
            A+FHET+R+YSP  +IPL
Sbjct: 373  AVFHETIRKYSPAPVIPL 390



 Score = 85.5 bits (210), Expect = 3e-14
 Identities = 40/78 (51%), Positives = 54/78 (69%)
 Frame = -3

Query: 356 RFLDSKFDPTDMFKTMAFGAGKRVCSGSQQAMTLSCLAIGAFVQEFEWRLSEGQKEEFNT 177
           RFL+  +D  D+ KTMAFGAGKR C+G+ QA  ++  +IG  VQEFEW L  G++E+ + 
Sbjct: 434 RFLNGNYDSGDLHKTMAFGAGKRACAGALQASLIASTSIGRLVQEFEWGLKAGEEEDVDI 493

Query: 176 LGLTSYKLHALMAFITPR 123
           +GLT+ KLH L   I PR
Sbjct: 494 VGLTTRKLHPLHLSIKPR 511