BLASTX nr result
ID: Aconitum21_contig00002557
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Aconitum21_contig00002557
(538 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002881339.1| CYP710A1 [Arabidopsis lyrata subsp. lyrata] ... 193 2e-60
ref|NP_180997.1| cytochrome P450, family 710, subfamily A [Arabi... 191 1e-58
ref|XP_002276613.1| PREDICTED: cytochrome P450 710A1-like [Vitis... 212 3e-53
ref|NP_001042373.1| Os01g0211200 [Oryza sativa Japonica Group] g... 179 5e-53
ref|NP_001234514.1| sterol 22-desaturase [Solanum lycopersicum] ... 206 2e-51
>ref|XP_002881339.1| CYP710A1 [Arabidopsis lyrata subsp. lyrata]
gi|297327178|gb|EFH57598.1| CYP710A1 [Arabidopsis lyrata
subsp. lyrata]
Length = 497
Score = 193 bits (491), Expect(2) = 2e-60
Identities = 95/138 (68%), Positives = 111/138 (80%), Gaps = 2/138 (1%)
Frame = +3
Query: 129 PDAFHLIGHPFGKKLFGEHNLIYMSGQEHKDLRRQIAPNFTPKALSTYIALQQVIILQHI 308
PDAFHLIGHPFGKKLFG+HNLIYM G++HK +RRQ+APNFTPKALSTY ALQQ++IL+H+
Sbjct: 101 PDAFHLIGHPFGKKLFGDHNLIYMFGEDHKSVRRQLAPNFTPKALSTYSALQQLVILRHL 160
Query: 309 KQWVE--LAGKSSIELRFLCFLCREMNLETSQTVFVGPYLGAEAKEGFNRDYNLFNVGLM 482
++W E G + LR L RE+NLETSQTVFVGPYL EAK+ F DYNLFN+G M
Sbjct: 161 RRWEESTSGGSRPVSLR---HLVRELNLETSQTVFVGPYLDKEAKKSFRTDYNLFNLGSM 217
Query: 483 NLPIDLPGFGFRKARLAV 536
LPIDLPGF F +AR AV
Sbjct: 218 ALPIDLPGFSFGEARRAV 235
Score = 64.7 bits (156), Expect(2) = 2e-60
Identities = 27/44 (61%), Positives = 38/44 (86%)
Frame = +1
Query: 1 TEFWVTQAAAARKTGVSANYIIGKFIVFVQNTELSHKVLANVRP 132
T FW Q+A A K+G+SANY+IGKFIV++++TELSH++ +NVRP
Sbjct: 58 TSFWDKQSATAGKSGLSANYLIGKFIVYIRDTELSHQIFSNVRP 101
>ref|NP_180997.1| cytochrome P450, family 710, subfamily A [Arabidopsis thaliana]
gi|75278303|sp|O64697.1|C7101_ARATH RecName:
Full=Cytochrome P450 710A1; AltName: Full=C-22 sterol
desaturase gi|3128210|gb|AAC26690.1| putative cytochrome
P450 [Arabidopsis thaliana] gi|20197156|gb|AAM14944.1|
putative cytochrome P450 [Arabidopsis thaliana]
gi|25083451|gb|AAN72080.1| putative cytochrome P450
[Arabidopsis thaliana] gi|34365731|gb|AAQ65177.1|
At2g34500 [Arabidopsis thaliana]
gi|84468537|dbj|BAE71351.1| CYP710 [Arabidopsis
thaliana] gi|330253888|gb|AEC08982.1| cytochrome P450,
family 710, subfamily A [Arabidopsis thaliana]
Length = 495
Score = 191 bits (484), Expect(2) = 1e-58
Identities = 95/138 (68%), Positives = 109/138 (78%), Gaps = 2/138 (1%)
Frame = +3
Query: 129 PDAFHLIGHPFGKKLFGEHNLIYMSGQEHKDLRRQIAPNFTPKALSTYIALQQVIILQHI 308
PDAFHLIGHPFGKKLFG+HNLIYM G++HK +RRQ+APNFTPKALSTY ALQQ++IL+H+
Sbjct: 101 PDAFHLIGHPFGKKLFGDHNLIYMFGEDHKSVRRQLAPNFTPKALSTYSALQQLVILRHL 160
Query: 309 KQW--VELAGKSSIELRFLCFLCREMNLETSQTVFVGPYLGAEAKEGFNRDYNLFNVGLM 482
+QW G + LR L RE+NLETSQTVFVGPYL EAK F DYNLFN+G M
Sbjct: 161 RQWEGSTSGGSRPVSLR---QLVRELNLETSQTVFVGPYLDKEAKNRFRTDYNLFNLGSM 217
Query: 483 NLPIDLPGFGFRKARLAV 536
LPIDLPGF F +AR AV
Sbjct: 218 ALPIDLPGFAFGEARRAV 235
Score = 61.2 bits (147), Expect(2) = 1e-58
Identities = 25/44 (56%), Positives = 37/44 (84%)
Frame = +1
Query: 1 TEFWVTQAAAARKTGVSANYIIGKFIVFVQNTELSHKVLANVRP 132
T FW Q++ A +G+SANY+IGKFIV++++TELSH++ +NVRP
Sbjct: 58 TSFWDKQSSTANISGLSANYLIGKFIVYIRDTELSHQIFSNVRP 101
>ref|XP_002276613.1| PREDICTED: cytochrome P450 710A1-like [Vitis vinifera]
Length = 509
Score = 212 bits (539), Expect = 3e-53
Identities = 101/136 (74%), Positives = 114/136 (83%)
Frame = +3
Query: 129 PDAFHLIGHPFGKKLFGEHNLIYMSGQEHKDLRRQIAPNFTPKALSTYIALQQVIILQHI 308
PDAFHL+GHPFGKKLFGEHNLIY GQ+HKD+RR+IAPNFTP+AL+ Y +LQQVIIL+H+
Sbjct: 106 PDAFHLVGHPFGKKLFGEHNLIYTMGQQHKDIRRRIAPNFTPRALAAYTSLQQVIILKHL 165
Query: 309 KQWVELAGKSSIELRFLCFLCREMNLETSQTVFVGPYLGAEAKEGFNRDYNLFNVGLMNL 488
W LA K+S L LCREMNL+TSQTVFVGPYL EA+E FNRDYNLFNVGLM L
Sbjct: 166 MAWEALASKASPTPISLRLLCREMNLDTSQTVFVGPYLSQEARERFNRDYNLFNVGLMKL 225
Query: 489 PIDLPGFGFRKARLAV 536
P DLPGF FR+ARLAV
Sbjct: 226 PFDLPGFAFREARLAV 241
Score = 67.0 bits (162), Expect = 2e-09
Identities = 37/86 (43%), Positives = 51/86 (59%), Gaps = 2/86 (2%)
Frame = +1
Query: 1 TEFWVTQAAAARKT--GVSANYIIGKFIVFVQNTELSHKVLANVRPTPSTSSATPLVRSF 174
T+FW Q++ AR + G+SANYI+GKFIVF+++T+LSHK+ ANVRP P +
Sbjct: 61 TKFWDIQSSLARSSDLGISANYIVGKFIVFIRSTDLSHKIFANVRPDAFHLVGHPFGKKL 120
Query: 175 LASTTLSTCRGKNTKISADKSRRISP 252
L G+ K D RRI+P
Sbjct: 121 FGEHNLIYTMGQQHK---DIRRRIAP 143
>ref|NP_001042373.1| Os01g0211200 [Oryza sativa Japonica Group]
gi|8096581|dbj|BAA96154.1| putative sterol C-22
desaturase [Oryza sativa Japonica Group]
gi|8096624|dbj|BAA96196.1| putative sterol C-22
desaturase [Oryza sativa Japonica Group]
gi|113531904|dbj|BAF04287.1| Os01g0211200 [Oryza sativa
Japonica Group] gi|125524878|gb|EAY72992.1| hypothetical
protein OsI_00865 [Oryza sativa Indica Group]
gi|125569491|gb|EAZ11006.1| hypothetical protein
OsJ_00850 [Oryza sativa Japonica Group]
Length = 504
Score = 179 bits (454), Expect(2) = 5e-53
Identities = 83/136 (61%), Positives = 106/136 (77%)
Frame = +3
Query: 129 PDAFHLIGHPFGKKLFGEHNLIYMSGQEHKDLRRQIAPNFTPKALSTYIALQQVIILQHI 308
PDAF +IGHPFGKKLFG+HNLIY+ G +HKDLRR++A NFTP+ALSTY A+QQ I+ H+
Sbjct: 107 PDAFFVIGHPFGKKLFGDHNLIYLFGDDHKDLRRRMATNFTPRALSTYAAIQQRGIVSHL 166
Query: 309 KQWVELAGKSSIELRFLCFLCREMNLETSQTVFVGPYLGAEAKEGFNRDYNLFNVGLMNL 488
++W++ + + + + CR+MNLETSQTVF GPYL EA+E F DYNLFNVGL+
Sbjct: 167 RRWLDRSAANGGKAEPIRVPCRDMNLETSQTVFAGPYLTEEARERFKSDYNLFNVGLLAF 226
Query: 489 PIDLPGFGFRKARLAV 536
P+DLPG FR+AR AV
Sbjct: 227 PVDLPGLAFRRARQAV 242
Score = 53.9 bits (128), Expect(2) = 5e-53
Identities = 25/46 (54%), Positives = 37/46 (80%), Gaps = 2/46 (4%)
Frame = +1
Query: 1 TEFWVTQAAAARKTGV--SANYIIGKFIVFVQNTELSHKVLANVRP 132
T FW QAA ARK+GV +A+++ GKF +F++++ELS++V ANVRP
Sbjct: 62 TGFWEMQAARARKSGVGFTADFLAGKFTIFIRDSELSNRVFANVRP 107
>ref|NP_001234514.1| sterol 22-desaturase [Solanum lycopersicum]
gi|161701428|gb|ABX75534.1| sterol 22-desaturase
[Solanum lycopersicum] gi|377657500|gb|AFB74183.1|
sterol 22-desaturase [Solanum lycopersicum]
gi|377657502|gb|AFB74184.1| sterol 22-desaturase
[Solanum lycopersicum] gi|377657504|gb|AFB74185.1|
sterol 22-desaturase [Solanum lycopersicum]
gi|377657506|gb|AFB74186.1| sterol 22-desaturase
[Solanum lycopersicum] gi|377657508|gb|AFB74187.1|
sterol 22-desaturase [Solanum lycopersicum]
gi|377657510|gb|AFB74188.1| sterol 22-desaturase
[Solanum lycopersicum] gi|377657512|gb|AFB74189.1|
sterol 22-desaturase [Solanum lycopersicum]
gi|377657514|gb|AFB74190.1| sterol 22-desaturase
[Solanum lycopersicum]
Length = 501
Score = 206 bits (523), Expect = 2e-51
Identities = 102/155 (65%), Positives = 116/155 (74%)
Frame = +3
Query: 72 VHRLCAEHRALA*STRQCPPDAFHLIGHPFGKKLFGEHNLIYMSGQEHKDLRRQIAPNFT 251
+H H+ A PDAFHLIGHPFGKKLFGEHNLIYM GQEHKDLRR+IAPNFT
Sbjct: 88 IHSTDLSHKVFA----NVRPDAFHLIGHPFGKKLFGEHNLIYMFGQEHKDLRRRIAPNFT 143
Query: 252 PKALSTYIALQQVIILQHIKQWVELAGKSSIELRFLCFLCREMNLETSQTVFVGPYLGAE 431
PKAL TY +QQ II++H K W++ A KS L LCR+MNL+TSQTVFVGPYL E
Sbjct: 144 PKALGTYTDIQQRIIIKHFKSWLDEASKSPNTPIPLRLLCRDMNLDTSQTVFVGPYLDGE 203
Query: 432 AKEGFNRDYNLFNVGLMNLPIDLPGFGFRKARLAV 536
+++ FN DYN FNVGLM LP+DLPGF FR ARLAV
Sbjct: 204 SRKRFNVDYNYFNVGLMKLPVDLPGFAFRNARLAV 238
Score = 63.2 bits (152), Expect = 2e-08
Identities = 37/86 (43%), Positives = 48/86 (55%), Gaps = 2/86 (2%)
Frame = +1
Query: 1 TEFWVTQAAAARKT--GVSANYIIGKFIVFVQNTELSHKVLANVRPTPSTSSATPLVRSF 174
T+FW Q+A A+ T G S NYIIGKFI+++ +T+LSHKV ANVRP P +
Sbjct: 58 TKFWDLQSALAKSTSHGFSVNYIIGKFILYIHSTDLSHKVFANVRPDAFHLIGHPFGKKL 117
Query: 175 LASTTLSTCRGKNTKISADKSRRISP 252
L G+ K D RRI+P
Sbjct: 118 FGEHNLIYMFGQEHK---DLRRRIAP 140