BLASTX nr result
ID: Aconitum21_contig00000690
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Aconitum21_contig00000690
(1590 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AFD54196.1| ent-kaurene oxidase [Vitis vinifera] 611 e-172
ref|XP_002282197.1| PREDICTED: ent-kaurene oxidase, chloroplasti... 608 e-171
gb|AEK01241.1| ent-kaurene oxidase [Pyrus communis] 604 e-170
gb|AEF32084.1| ent-kaurene oxidase [Castanea mollissima] 598 e-168
gb|AAG41776.1|AF212990_1 ent-kaurene oxidase [Cucurbita maxima] 594 e-167
>gb|AFD54196.1| ent-kaurene oxidase [Vitis vinifera]
Length = 508
Score = 611 bits (1575), Expect = e-172
Identities = 290/456 (63%), Positives = 374/456 (82%)
Frame = +1
Query: 1 IGNLHQLSDKKPHQTFTKWAEIYGPIYSIKTGYSTLVVLNSNDVAKEAMVTRFSSISTRK 180
IGNL QL +KKPH+TF +WAE YGPI+SI+TG ST++VLNS++VAKEAMVTRFSSISTRK
Sbjct: 54 IGNLLQLKEKKPHKTFARWAETYGPIFSIRTGASTMIVLNSSEVAKEAMVTRFSSISTRK 113
Query: 181 LSNALTILTLDKKIVAISDYGDFHKITKKYLISGMLGANAQKRYRGHRETMISNMMRKLY 360
LSNAL ILT DK +VA SDY DFHK+ K +++ +LGA AQKR+R HR+T+I N+ + L+
Sbjct: 114 LSNALKILTFDKCMVATSDYNDFHKMVKGFILRNVLGAPAQKRHRCHRDTLIENISKYLH 173
Query: 361 AHNEEKPLETVNLRSIFQHELFGLAMKQAYGRDLEDEVYIEGLGTKLSRYEIFEALVVDP 540
AH + PLE V L+ IF+ E+FGLA+KQA G+D+E +Y+E LGT LSR EIF LVVDP
Sbjct: 174 AHVKTSPLEPVVLKKIFESEIFGLALKQALGKDIES-IYVEELGTTLSREEIFAVLVVDP 232
Query: 541 MMGAIAVDWRDFFPYLRWIPNKGLEARIERMAFRRRAVCKALIDAQKRRRASGEILDSYV 720
M GAI VDWRDFFPYL WIPNK +E +I+RM FRR A+ KALI QK+R SGE +SY+
Sbjct: 233 MAGAIEVDWRDFFPYLSWIPNKSMEMKIQRMDFRRGALMKALIGEQKKRIGSGEEKNSYI 292
Query: 721 DYLLAPDLKQFTEDELIMLMWEVVIETSDTTLVTTEWAMYEISKNRRVQELLHRELKEVC 900
D+LL+ + TE ++ ML+WE +IE SDTTLVT+EWAMYE++K+ QE+L+RE+ +VC
Sbjct: 293 DFLLS-EATTLTEKQIAMLIWETIIEISDTTLVTSEWAMYELAKDPNRQEILYREIHKVC 351
Query: 901 GSEKVTEDHLPRLPYLNAIFHETLRRHSPAPMIPLRYVHEDTELGGYHIPAGTQISINIY 1080
GS K+TE++L +LPYLN++FHETLR++SPAPM+P+RY HEDT+LGGYHIPAG+QI+INIY
Sbjct: 352 GSNKLTEENLSKLPYLNSVFHETLRKYSPAPMVPVRYAHEDTQLGGYHIPAGSQIAINIY 411
Query: 1081 GCNMDKKQWDEPDAWKPERFLDPKFDPTDMFKSLAFGGGKRICAGAQQAMTIACMAIATY 1260
GCNM+KKQW+ P+ WKPERFLD K+D D+ K++AFGGGKR+CAGA QAM IAC +I +
Sbjct: 412 GCNMNKKQWENPEEWKPERFLDEKYDLMDLHKTMAFGGGKRVCAGALQAMLIACTSIGRF 471
Query: 1261 VQEFDWKLDEGQKEDVNTLGLTSYRLYPLQVHIKPR 1368
VQEF+WKL G++E+V+T+ LTS +L+P+Q IK R
Sbjct: 472 VQEFEWKLMGGEEENVDTVALTSQKLHPMQAIIKAR 507
>ref|XP_002282197.1| PREDICTED: ent-kaurene oxidase, chloroplastic [Vitis vinifera]
gi|302142385|emb|CBI19588.3| unnamed protein product
[Vitis vinifera]
Length = 508
Score = 608 bits (1567), Expect = e-171
Identities = 288/456 (63%), Positives = 373/456 (81%)
Frame = +1
Query: 1 IGNLHQLSDKKPHQTFTKWAEIYGPIYSIKTGYSTLVVLNSNDVAKEAMVTRFSSISTRK 180
IGNL QL +KKPH+TF +WAE YGPI+SI+TG ST++VLNS++VAKEAMVTRFSSISTRK
Sbjct: 54 IGNLLQLKEKKPHKTFARWAETYGPIFSIRTGASTMIVLNSSEVAKEAMVTRFSSISTRK 113
Query: 181 LSNALTILTLDKKIVAISDYGDFHKITKKYLISGMLGANAQKRYRGHRETMISNMMRKLY 360
LSNAL ILT DK +VA SDY DFHK+ K +++ +LGA AQKR+R HR+T+I N+ + L+
Sbjct: 114 LSNALKILTFDKCMVATSDYNDFHKMVKGFILRNVLGAPAQKRHRCHRDTLIENISKYLH 173
Query: 361 AHNEEKPLETVNLRSIFQHELFGLAMKQAYGRDLEDEVYIEGLGTKLSRYEIFEALVVDP 540
AH + PLE V L+ IF+ E+FGLA+KQA G+D+E +Y+E LGT LSR EIF LVVDP
Sbjct: 174 AHVKTSPLEPVVLKKIFESEIFGLALKQALGKDIES-IYVEELGTTLSREEIFAVLVVDP 232
Query: 541 MMGAIAVDWRDFFPYLRWIPNKGLEARIERMAFRRRAVCKALIDAQKRRRASGEILDSYV 720
M GAI VDWRDFFPYL WIPNK +E +I+RM FRR A+ KALI QK+R SGE + Y+
Sbjct: 233 MAGAIEVDWRDFFPYLSWIPNKSMEMKIQRMDFRRGALMKALIGEQKKRIGSGEEKNCYI 292
Query: 721 DYLLAPDLKQFTEDELIMLMWEVVIETSDTTLVTTEWAMYEISKNRRVQELLHRELKEVC 900
D+LL+ + TE ++ ML+WE +IE SDTTLVT+EWAMYE++K+ QE+L+RE+++ C
Sbjct: 293 DFLLS-EATTLTEKQIAMLIWETIIEISDTTLVTSEWAMYELAKDPNRQEILYREIRKFC 351
Query: 901 GSEKVTEDHLPRLPYLNAIFHETLRRHSPAPMIPLRYVHEDTELGGYHIPAGTQISINIY 1080
GS KVTE++L +LPYLN++FHETLR++SPAP++P+RY HEDT+LGGYHIPAG+QI+INIY
Sbjct: 352 GSNKVTEENLSKLPYLNSVFHETLRKYSPAPIVPVRYAHEDTQLGGYHIPAGSQIAINIY 411
Query: 1081 GCNMDKKQWDEPDAWKPERFLDPKFDPTDMFKSLAFGGGKRICAGAQQAMTIACMAIATY 1260
GCNM+KKQW+ P+ WKPERFLD K+D D+ K++AFGGGKR+CAGA QAM IAC +I +
Sbjct: 412 GCNMNKKQWENPEEWKPERFLDEKYDLMDLHKTMAFGGGKRVCAGALQAMLIACTSIGRF 471
Query: 1261 VQEFDWKLDEGQKEDVNTLGLTSYRLYPLQVHIKPR 1368
VQEF+WKL G++E+V+T+ LTS +L+P+Q IK R
Sbjct: 472 VQEFEWKLMGGEEENVDTVALTSQKLHPMQAIIKAR 507
>gb|AEK01241.1| ent-kaurene oxidase [Pyrus communis]
Length = 514
Score = 604 bits (1558), Expect = e-170
Identities = 278/456 (60%), Positives = 370/456 (81%)
Frame = +1
Query: 1 IGNLHQLSDKKPHQTFTKWAEIYGPIYSIKTGYSTLVVLNSNDVAKEAMVTRFSSISTRK 180
+GNL QL +KKP++TFT+WAE YGPIYSI+TG +T+VVLN+ DVAKEAMVTR+SS+STRK
Sbjct: 59 VGNLLQLKEKKPYKTFTRWAEEYGPIYSIRTGATTMVVLNTADVAKEAMVTRYSSMSTRK 118
Query: 181 LSNALTILTLDKKIVAISDYGDFHKITKKYLISGMLGANAQKRYRGHRETMISNMMRKLY 360
LS AL ILT DK +VA SDY DFH + K+Y+++ +LGANAQKR+R R+TM N+ +L+
Sbjct: 119 LSKALKILTFDKCMVATSDYNDFHNMVKRYVLTNVLGANAQKRHRSQRDTMRENVASRLH 178
Query: 361 AHNEEKPLETVNLRSIFQHELFGLAMKQAYGRDLEDEVYIEGLGTKLSRYEIFEALVVDP 540
AH P E +N R IF+ ELFG+A+KQA G+D+ED + +E LGT LSR +IF+ LV D
Sbjct: 179 AHVNNSPQEAINFREIFESELFGIALKQAIGKDIEDSINVEELGTTLSRDDIFKVLVNDM 238
Query: 541 MMGAIAVDWRDFFPYLRWIPNKGLEARIERMAFRRRAVCKALIDAQKRRRASGEILDSYV 720
M GAI VDWRDFFPYLRW+P K +E ++R++ RR AV LID QK+R ASGE LD ++
Sbjct: 239 MEGAIEVDWRDFFPYLRWVPIKTIENNMKRLSRRRIAVMNVLIDEQKKRIASGEELDCHI 298
Query: 721 DYLLAPDLKQFTEDELIMLMWEVVIETSDTTLVTTEWAMYEISKNRRVQELLHRELKEVC 900
DYLL+ + K T +++ ML+WE +IET+DTTLVTTEW MYE++K+++ Q+ L E++ VC
Sbjct: 299 DYLLS-EAKTLTPEQISMLIWETIIETADTTLVTTEWGMYELAKDQKRQDRLFEEVQNVC 357
Query: 901 GSEKVTEDHLPRLPYLNAIFHETLRRHSPAPMIPLRYVHEDTELGGYHIPAGTQISINIY 1080
G+ KVTE+HL ++PYL A+FHETLR+HSPAP++PLRYVHEDTELGGY++P GT+I+INIY
Sbjct: 358 GTNKVTEEHLSQMPYLGAVFHETLRKHSPAPIVPLRYVHEDTELGGYYVPVGTEIAINIY 417
Query: 1081 GCNMDKKQWDEPDAWKPERFLDPKFDPTDMFKSLAFGGGKRICAGAQQAMTIACMAIATY 1260
GCNMDK QW+ P+ WKPERFLDPK+DP D++K++AFG GKR+CAG+ QAM IAC I
Sbjct: 418 GCNMDKNQWESPEEWKPERFLDPKYDPMDLYKTMAFGTGKRVCAGSLQAMLIACTTIGRL 477
Query: 1261 VQEFDWKLDEGQKEDVNTLGLTSYRLYPLQVHIKPR 1368
VQEF+WKL +G++E+V+T+GLT+++L+P+ +KPR
Sbjct: 478 VQEFEWKLRDGEEENVDTVGLTTHKLHPMHAILKPR 513
>gb|AEF32084.1| ent-kaurene oxidase [Castanea mollissima]
Length = 514
Score = 598 bits (1542), Expect = e-168
Identities = 273/456 (59%), Positives = 374/456 (82%)
Frame = +1
Query: 1 IGNLHQLSDKKPHQTFTKWAEIYGPIYSIKTGYSTLVVLNSNDVAKEAMVTRFSSISTRK 180
+GNL QL +KKP++TF +WAEI+GPIYSI+TG ST+VV+NS VAKEAMVTRFSSISTRK
Sbjct: 60 VGNLLQLKEKKPYKTFLRWAEIHGPIYSIRTGASTMVVVNSTHVAKEAMVTRFSSISTRK 119
Query: 181 LSNALTILTLDKKIVAISDYGDFHKITKKYLISGMLGANAQKRYRGHRETMISNMMRKLY 360
LS AL +LT +K +VA SDY +FHK+ KKY+++ +LGANAQKR+R HR+T+I N++ KL+
Sbjct: 120 LSKALELLTSNKSMVATSDYNEFHKMVKKYILAELLGANAQKRHRIHRDTLIENVLNKLH 179
Query: 361 AHNEEKPLETVNLRSIFQHELFGLAMKQAYGRDLEDEVYIEGLGTKLSRYEIFEALVVDP 540
AH + PL+ VN R IF+ ELFGLAMKQA G D+ D +++E LGT LSR EI+ LV D
Sbjct: 180 AHTKNSPLQAVNFRKIFESELFGLAMKQALGYDV-DSLFVEELGTTLSREEIYNVLVSDM 238
Query: 541 MMGAIAVDWRDFFPYLRWIPNKGLEARIERMAFRRRAVCKALIDAQKRRRASGEILDSYV 720
+ GAI VDWRDFFPYL+WIPNK E +I+R+A RR+AV +++ QK+ ASG+ + Y+
Sbjct: 239 LKGAIEVDWRDFFPYLKWIPNKSFEMKIQRLASRRQAVMNSIVKEQKKSIASGKGENCYL 298
Query: 721 DYLLAPDLKQFTEDELIMLMWEVVIETSDTTLVTTEWAMYEISKNRRVQELLHRELKEVC 900
+YLL+ + K TE ++ +L WE +IET+DTT+VTTEWAMYE++KN + Q+ L+ E++ VC
Sbjct: 299 NYLLS-EAKTLTEKQISILAWETIIETADTTVVTTEWAMYELAKNPKQQDRLYNEIQNVC 357
Query: 901 GSEKVTEDHLPRLPYLNAIFHETLRRHSPAPMIPLRYVHEDTELGGYHIPAGTQISINIY 1080
G++K+TE+HL +LPYL+A+FHETLR++SP+P++PLRY HEDT+LGGY++PAGT+I++NIY
Sbjct: 358 GTDKITEEHLSKLPYLSAVFHETLRKYSPSPLVPLRYAHEDTQLGGYYVPAGTEIAVNIY 417
Query: 1081 GCNMDKKQWDEPDAWKPERFLDPKFDPTDMFKSLAFGGGKRICAGAQQAMTIACMAIATY 1260
GCNMDK QW+ P+ WKPERFLD K+DP DM+K+++FG GKR+CAG+ QA IAC +I
Sbjct: 418 GCNMDKNQWETPEEWKPERFLDEKYDPMDMYKTMSFGSGKRVCAGSLQASLIACTSIGRL 477
Query: 1261 VQEFDWKLDEGQKEDVNTLGLTSYRLYPLQVHIKPR 1368
VQEF+W+L +G+ E+V+TLGLT+++LYP+Q ++PR
Sbjct: 478 VQEFEWRLKDGEVENVDTLGLTTHKLYPMQAILQPR 513
>gb|AAG41776.1|AF212990_1 ent-kaurene oxidase [Cucurbita maxima]
Length = 517
Score = 594 bits (1532), Expect = e-167
Identities = 273/456 (59%), Positives = 370/456 (81%)
Frame = +1
Query: 1 IGNLHQLSDKKPHQTFTKWAEIYGPIYSIKTGYSTLVVLNSNDVAKEAMVTRFSSISTRK 180
+GNL QL +KKPH+TF +W+E YGPIYSIK G ST++VLNS+D+AKEAMVTR+SSIS+RK
Sbjct: 62 LGNLLQLKEKKPHKTFAQWSETYGPIYSIKAGASTVIVLNSSDLAKEAMVTRYSSISSRK 121
Query: 181 LSNALTILTLDKKIVAISDYGDFHKITKKYLISGMLGANAQKRYRGHRETMISNMMRKLY 360
LS ALTILT DK +VA+SDY DFHK+ K+Y+++ +LGANAQKR R R+TMI N+ R+L+
Sbjct: 122 LSKALTILTADKCMVAMSDYNDFHKLVKRYILANVLGANAQKRLRQRRDTMIDNISRELF 181
Query: 361 AHNEEKPLETVNLRSIFQHELFGLAMKQAYGRDLEDEVYIEGLGTKLSRYEIFEALVVDP 540
A ++ E+VN R IF+ ELFGLA+K+ +GRD+E +Y++GLGT L R ++F LV+DP
Sbjct: 182 ACVKDSSSESVNFRKIFESELFGLALKETFGRDMES-LYVDGLGTTLLREDLFRTLVIDP 240
Query: 541 MMGAIAVDWRDFFPYLRWIPNKGLEARIERMAFRRRAVCKALIDAQKRRRASGEILDSYV 720
M GAI VDWRDFFPYLRWIPNKG+E RI +M FRRR K+L++ +K++ A+GE L+ Y
Sbjct: 241 MEGAIEVDWRDFFPYLRWIPNKGVEDRIRKMDFRRRVTMKSLMEEKKKQIAAGEDLNCYS 300
Query: 721 DYLLAPDLKQFTEDELIMLMWEVVIETSDTTLVTTEWAMYEISKNRRVQELLHRELKEVC 900
++LL+ + K TE+++ ML+WE++IETSDTTLV TEWAMYE+++N + QE L++ ++ VC
Sbjct: 301 EFLLS-EAKSLTEEQISMLLWEIIIETSDTTLVVTEWAMYELAQNPKRQERLYQHIQSVC 359
Query: 901 GSEKVTEDHLPRLPYLNAIFHETLRRHSPAPMIPLRYVHEDTELGGYHIPAGTQISINIY 1080
GS K+TE++L +LPYL A+FHETLR++SP ++PLRY HEDT+LGGY IPAG+++++NIY
Sbjct: 360 GSAKITEENLSQLPYLTAVFHETLRKYSPVSIVPLRYAHEDTQLGGYFIPAGSEVAVNIY 419
Query: 1081 GCNMDKKQWDEPDAWKPERFLDPKFDPTDMFKSLAFGGGKRICAGAQQAMTIACMAIATY 1260
CNMDKKQW+ P+ WKPERFLD +DP D++K++AFGGGKR+CAGA +AM IAC +
Sbjct: 420 ACNMDKKQWESPEEWKPERFLDESYDPMDLYKTMAFGGGKRVCAGAPKAMLIACTTLGRL 479
Query: 1261 VQEFDWKLDEGQKEDVNTLGLTSYRLYPLQVHIKPR 1368
VQ F WKL EG+++ V+TLGLT+ +L PL + KPR
Sbjct: 480 VQGFTWKLREGEEDKVDTLGLTARKLQPLHIVAKPR 515