BLASTX nr result
ID: Achyranthes23_contig00055346
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Achyranthes23_contig00055346
(304 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AGG82699.1| endo-1,4-beta-mannosidase [Morus alba var. multic... 138 7e-31
gb|EXC25069.1| Mannan endo-1,4-beta-mannosidase 7 [Morus notabilis] 137 1e-30
gb|EOY14814.1| Glycosyl hydrolase superfamily protein [Theobroma... 135 6e-30
gb|EMJ23584.1| hypothetical protein PRUPE_ppa006029mg [Prunus pe... 134 1e-29
gb|ABV32547.1| endo-1,4-beta-mannosidase protein 1 [Prunus persica] 134 1e-29
ref|XP_004291857.1| PREDICTED: mannan endo-1,4-beta-mannosidase ... 133 3e-29
ref|XP_002281804.1| PREDICTED: mannan endo-1,4-beta-mannosidase ... 132 4e-29
gb|ACN78664.1| endo-beta-mannanase [Malus domestica] 131 1e-28
ref|XP_006393839.1| hypothetical protein EUTSA_v10004263mg [Eutr... 129 4e-28
ref|XP_002510418.1| hydrolase, hydrolyzing O-glycosyl compounds,... 128 7e-28
gb|ACN78666.1| endo-beta-mannanase [Vaccinium corymbosum] 127 2e-27
ref|XP_003517214.1| PREDICTED: mannan endo-1,4-beta-mannosidase ... 127 2e-27
ref|XP_002306881.1| hypothetical protein POPTR_0005s25120g [Popu... 127 2e-27
ref|XP_002310816.2| hypothetical protein POPTR_0007s13190g [Popu... 126 3e-27
ref|XP_002327685.1| predicted protein [Populus trichocarpa] gi|5... 126 3e-27
gb|ESW28974.1| hypothetical protein PHAVU_002G033500g [Phaseolus... 126 3e-27
ref|XP_006340651.1| PREDICTED: mannan endo-1,4-beta-mannosidase ... 125 4e-27
ref|XP_003528027.1| PREDICTED: mannan endo-1,4-beta-mannosidase ... 125 6e-27
ref|XP_004512071.1| PREDICTED: mannan endo-1,4-beta-mannosidase ... 125 8e-27
ref|XP_004143522.1| PREDICTED: mannan endo-1,4-beta-mannosidase ... 125 8e-27
>gb|AGG82699.1| endo-1,4-beta-mannosidase [Morus alba var. multicaulis]
Length = 420
Score = 138 bits (348), Expect = 7e-31
Identities = 66/101 (65%), Positives = 80/101 (79%)
Frame = +1
Query: 1 KMKTWGLTLLILLPTIQEIIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEASYPSQR 180
K + WG+ L LL + +++AEDGF+ T+GVQ +LN PFY NGFNAYWLMY AS PSQR
Sbjct: 2 KKEQWGVVLFSLL-SFHQVLAEDGFVKTRGVQLVLNGYPFYANGFNAYWLMYIASDPSQR 60
Query: 181 NKVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
+KVS+AFQEA +GL+IARTWAFSD G PLQ SPGSYNE+
Sbjct: 61 DKVSSAFQEAKNHGLSIARTWAFSDGGYMPLQYSPGSYNEK 101
>gb|EXC25069.1| Mannan endo-1,4-beta-mannosidase 7 [Morus notabilis]
Length = 420
Score = 137 bits (346), Expect = 1e-30
Identities = 66/101 (65%), Positives = 79/101 (78%)
Frame = +1
Query: 1 KMKTWGLTLLILLPTIQEIIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEASYPSQR 180
K + WG+ L LL + ++ AEDGF+ T+GVQ +LN PFY NGFNAYWLMY AS PSQR
Sbjct: 2 KKEQWGVVLFSLL-SFHQVFAEDGFVKTRGVQLVLNGYPFYANGFNAYWLMYIASDPSQR 60
Query: 181 NKVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
+KVS+AFQEA +GL+IARTWAFSD G PLQ SPGSYNE+
Sbjct: 61 DKVSSAFQEAKNHGLSIARTWAFSDGGYMPLQYSPGSYNEK 101
>gb|EOY14814.1| Glycosyl hydrolase superfamily protein [Theobroma cacao]
Length = 431
Score = 135 bits (340), Expect = 6e-30
Identities = 65/105 (61%), Positives = 78/105 (74%), Gaps = 5/105 (4%)
Frame = +1
Query: 4 MKTWGLTLLILL-----PTIQEIIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEASY 168
MK WGL L + L + + A+DGF+ TKG+Q +L+ PFY NGFNAYWLMY A+
Sbjct: 1 MKQWGLVLFVFLLFQEQGSFVRVEADDGFVKTKGMQLMLDGSPFYANGFNAYWLMYMAAD 60
Query: 169 PSQRNKVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
PSQR+KVS AFQEA ++GL IARTWAFSD G RPLQ SPGSYNE+
Sbjct: 61 PSQRSKVSTAFQEAKQHGLNIARTWAFSDGGDRPLQYSPGSYNEQ 105
>gb|EMJ23584.1| hypothetical protein PRUPE_ppa006029mg [Prunus persica]
Length = 431
Score = 134 bits (337), Expect = 1e-29
Identities = 62/105 (59%), Positives = 81/105 (77%), Gaps = 5/105 (4%)
Frame = +1
Query: 4 MKTWGLTLLILL-----PTIQEIIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEASY 168
MK WG+ +LL ++ + AEDGF+ T+G+Q +LN +P+Y +GFNAYWLMY A+
Sbjct: 1 MKRWGVVFFVLLLIQSPGILKPVEAEDGFVKTRGLQLVLNGVPYYAHGFNAYWLMYVATD 60
Query: 169 PSQRNKVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
PSQR+K+ +AFQEA K+GL+IARTWAFSD G RPLQ SPGSYNE+
Sbjct: 61 PSQRDKILSAFQEATKHGLSIARTWAFSDGGYRPLQSSPGSYNEQ 105
>gb|ABV32547.1| endo-1,4-beta-mannosidase protein 1 [Prunus persica]
Length = 431
Score = 134 bits (337), Expect = 1e-29
Identities = 62/105 (59%), Positives = 81/105 (77%), Gaps = 5/105 (4%)
Frame = +1
Query: 4 MKTWGLTLLILL-----PTIQEIIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEASY 168
MK WG+ +LL ++ + AEDGF+ T+G+Q +LN +P+Y +GFNAYWLMY A+
Sbjct: 1 MKRWGVVFFVLLLIQSPGILRPVEAEDGFVKTRGLQLVLNGVPYYAHGFNAYWLMYVATD 60
Query: 169 PSQRNKVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
PSQR+K+ +AFQEA K+GL+IARTWAFSD G RPLQ SPGSYNE+
Sbjct: 61 PSQRDKILSAFQEATKHGLSIARTWAFSDGGYRPLQSSPGSYNEQ 105
>ref|XP_004291857.1| PREDICTED: mannan endo-1,4-beta-mannosidase 7-like [Fragaria vesca
subsp. vesca]
Length = 433
Score = 133 bits (334), Expect = 3e-29
Identities = 67/106 (63%), Positives = 78/106 (73%), Gaps = 6/106 (5%)
Frame = +1
Query: 4 MKTWGLTLLILL------PTIQEIIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEAS 165
MK+ GL LL +Q++ AED F+ TKGVQF+LN P+Y NGFNAYWLMY AS
Sbjct: 1 MKSCGLLFFALLLLQYHGVLVQQVEAEDDFVKTKGVQFMLNGTPYYANGFNAYWLMYMAS 60
Query: 166 YPSQRNKVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
PSQRN VS+AFQ+A KNGLTIARTWAF+D R LQ SPGSYNE+
Sbjct: 61 DPSQRNLVSSAFQQASKNGLTIARTWAFNDGDYRALQYSPGSYNEQ 106
>ref|XP_002281804.1| PREDICTED: mannan endo-1,4-beta-mannosidase 7 [Vitis vinifera]
gi|302142526|emb|CBI19729.3| unnamed protein product
[Vitis vinifera]
Length = 433
Score = 132 bits (333), Expect = 4e-29
Identities = 63/105 (60%), Positives = 79/105 (75%), Gaps = 5/105 (4%)
Frame = +1
Query: 4 MKTWGLTLLILL-----PTIQEIIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEASY 168
MK+W L LL+L+ ++ A DGF+ T G+ F+LN +P++ NGFNAYWLMY AS
Sbjct: 1 MKSWALALLVLVFIQKQGNFLQVEAADGFVKTSGMHFVLNGLPYHANGFNAYWLMYVASD 60
Query: 169 PSQRNKVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
PSQR+K+S+AF EA +GLTIARTWAFSD G RPLQ SPGSYNE+
Sbjct: 61 PSQRSKISSAFAEAAGHGLTIARTWAFSDGGYRPLQYSPGSYNEQ 105
>gb|ACN78664.1| endo-beta-mannanase [Malus domestica]
Length = 429
Score = 131 bits (329), Expect = 1e-28
Identities = 59/83 (71%), Positives = 72/83 (86%)
Frame = +1
Query: 55 IIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEASYPSQRNKVSNAFQEALKNGLTIA 234
+IAEDGF+ T+GVQ +LN +P+YGNGFNAYWLMY A+ PS+R KVS+AF+EA KNGLTIA
Sbjct: 21 VIAEDGFVKTRGVQLVLNGVPYYGNGFNAYWLMYMATDPSEREKVSSAFREATKNGLTIA 80
Query: 235 RTWAFSDAGSRPLQISPGSYNEE 303
RTWAF+D G R LQ SPGSY+E+
Sbjct: 81 RTWAFADGGYRALQTSPGSYDEK 103
>ref|XP_006393839.1| hypothetical protein EUTSA_v10004263mg [Eutrema salsugineum]
gi|78499698|gb|ABB45852.1| hypothetical protein [Eutrema
halophilum] gi|557090478|gb|ESQ31125.1| hypothetical
protein EUTSA_v10004263mg [Eutrema salsugineum]
Length = 431
Score = 129 bits (324), Expect = 4e-28
Identities = 63/107 (58%), Positives = 77/107 (71%), Gaps = 7/107 (6%)
Frame = +1
Query: 4 MKTWGLTLLILLPTIQ-------EIIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEA 162
MK GL + + + Q + + DGF+ TKGVQF LN P+Y NGFNAYWLMY A
Sbjct: 1 MKLLGLIIFLAIVIQQSYWELGADAFSSDGFVRTKGVQFSLNGYPYYANGFNAYWLMYVA 60
Query: 163 SYPSQRNKVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
S PSQR+K+S AFQEA ++GLT+ARTWAFSD G RPLQ +PGSYNE+
Sbjct: 61 SDPSQRSKISAAFQEASRHGLTVARTWAFSDGGYRPLQYTPGSYNED 107
>ref|XP_002510418.1| hydrolase, hydrolyzing O-glycosyl compounds, putative [Ricinus
communis] gi|223551119|gb|EEF52605.1| hydrolase,
hydrolyzing O-glycosyl compounds, putative [Ricinus
communis]
Length = 432
Score = 128 bits (322), Expect = 7e-28
Identities = 64/106 (60%), Positives = 76/106 (71%), Gaps = 6/106 (5%)
Frame = +1
Query: 4 MKTWGLTLLILLPT------IQEIIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEAS 165
MK WGL L +L +Q A+DGF++TKGVQF+LN PFY NGFNAYWLMY A+
Sbjct: 1 MKYWGLVFLAILVFQKQGMFLQVKAADDGFVSTKGVQFMLNGSPFYANGFNAYWLMYFAT 60
Query: 166 YPSQRNKVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
S RNKVS+ FQEA +GLT+ARTWAF+D R LQ SPGSYNE+
Sbjct: 61 DNSMRNKVSSVFQEARNHGLTLARTWAFNDGQDRALQYSPGSYNEQ 106
>gb|ACN78666.1| endo-beta-mannanase [Vaccinium corymbosum]
Length = 425
Score = 127 bits (319), Expect = 2e-27
Identities = 63/102 (61%), Positives = 77/102 (75%), Gaps = 2/102 (1%)
Frame = +1
Query: 4 MKTWGL--TLLILLPTIQEIIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEASYPSQ 177
MK GL ++L+L+ ++ A DGFI T+GVQF+LN P+Y NGFNAYWLMY A+ PS+
Sbjct: 1 MKHLGLLISVLLLIQNQVQVEAGDGFIKTRGVQFVLNGSPYYANGFNAYWLMYVATDPSE 60
Query: 178 RNKVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
RNKVS+ FQEA GLT+ARTWAF D G R LQ SPGSYNE+
Sbjct: 61 RNKVSSVFQEASSRGLTLARTWAFGDGGYRALQNSPGSYNEQ 102
>ref|XP_003517214.1| PREDICTED: mannan endo-1,4-beta-mannosidase 7-like [Glycine max]
Length = 431
Score = 127 bits (318), Expect = 2e-27
Identities = 62/105 (59%), Positives = 75/105 (71%), Gaps = 5/105 (4%)
Frame = +1
Query: 4 MKTWGLTLLILLPTIQE-----IIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEASY 168
MK + L L+ + QE + A D F+ T+G+ F+LN P+Y NGFNAYWLMY AS
Sbjct: 1 MKHFALVFLLAILVPQECFHVSVEARDDFVRTRGIHFMLNGYPYYANGFNAYWLMYTASD 60
Query: 169 PSQRNKVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
PSQR KVSNAF+EA +GLT+ARTWAFSD G RPLQ SPG YNE+
Sbjct: 61 PSQRFKVSNAFREAASHGLTVARTWAFSDGGYRPLQYSPGFYNEQ 105
>ref|XP_002306881.1| hypothetical protein POPTR_0005s25120g [Populus trichocarpa]
gi|222856330|gb|EEE93877.1| hypothetical protein
POPTR_0005s25120g [Populus trichocarpa]
Length = 420
Score = 127 bits (318), Expect = 2e-27
Identities = 63/106 (59%), Positives = 79/106 (74%), Gaps = 6/106 (5%)
Frame = +1
Query: 4 MKTWGLTLLILLPTIQE------IIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEAS 165
M+ WGL L++L IQE + A+DGFI TKGVQFLLN PFY NGFN YWLMY A+
Sbjct: 1 MRHWGLIFLVIL-LIQEQGIFLQVEADDGFIRTKGVQFLLNGSPFYANGFNGYWLMYFAT 59
Query: 166 YPSQRNKVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
SQR+KV++ FQ+A ++GLT+ARTWAF+D R LQ+SPG YNE+
Sbjct: 60 DTSQRDKVTSVFQDAKQHGLTLARTWAFNDGQDRALQVSPGHYNEQ 105
>ref|XP_002310816.2| hypothetical protein POPTR_0007s13190g [Populus trichocarpa]
gi|550334779|gb|EEE91266.2| hypothetical protein
POPTR_0007s13190g [Populus trichocarpa]
Length = 435
Score = 126 bits (317), Expect = 3e-27
Identities = 64/102 (62%), Positives = 75/102 (73%), Gaps = 7/102 (6%)
Frame = +1
Query: 19 LTLLILLPTIQE-------IIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEASYPSQ 177
LTL++LL + + A DGFI +GVQFLLN P+Y NGFNAYWLMY AS PSQ
Sbjct: 4 LTLVLLLTILIHQGCLHFHVEAGDGFIRARGVQFLLNGSPYYANGFNAYWLMYTASDPSQ 63
Query: 178 RNKVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
R+KVS AF+EA +GLT+ARTWAFSD G PLQ SPGSYNE+
Sbjct: 64 RSKVSAAFREAASHGLTVARTWAFSDGGYSPLQYSPGSYNEQ 105
>ref|XP_002327685.1| predicted protein [Populus trichocarpa]
gi|566170965|ref|XP_006383170.1| (1-4)-beta-mannan
endohydrolase family protein [Populus trichocarpa]
gi|118487673|gb|ABK95661.1| unknown [Populus
trichocarpa] gi|550338751|gb|ERP60967.1|
(1-4)-beta-mannan endohydrolase family protein [Populus
trichocarpa]
Length = 435
Score = 126 bits (317), Expect = 3e-27
Identities = 60/81 (74%), Positives = 66/81 (81%)
Frame = +1
Query: 61 AEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEASYPSQRNKVSNAFQEALKNGLTIART 240
A DGFI T+GV FLLN P+Y NGFNAYWLMY AS PSQR KVS AF+EA +GLT+ART
Sbjct: 25 AGDGFIRTRGVHFLLNGSPYYANGFNAYWLMYTASDPSQRPKVSAAFREAASHGLTVART 84
Query: 241 WAFSDAGSRPLQISPGSYNEE 303
WAFSD G RPLQ SPGSYNE+
Sbjct: 85 WAFSDGGYRPLQYSPGSYNEQ 105
>gb|ESW28974.1| hypothetical protein PHAVU_002G033500g [Phaseolus vulgaris]
Length = 431
Score = 126 bits (316), Expect = 3e-27
Identities = 62/105 (59%), Positives = 76/105 (72%), Gaps = 5/105 (4%)
Frame = +1
Query: 4 MKTWGLTLLILLPTIQE-----IIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEASY 168
MK + L L+ + QE + A DGF+ T+G+ F+LN P+Y NGFNAYWLMY AS
Sbjct: 1 MKHFVLVFLLAILAPQECFHATVEARDGFVRTRGIHFMLNGYPYYVNGFNAYWLMYTASD 60
Query: 169 PSQRNKVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
PSQR KVSNAF+EA +GLT+ARTWAFSD G RPLQ SPG YN++
Sbjct: 61 PSQRFKVSNAFREAASHGLTVARTWAFSDGGYRPLQYSPGFYNDQ 105
>ref|XP_006340651.1| PREDICTED: mannan endo-1,4-beta-mannosidase 7-like [Solanum
tuberosum]
Length = 433
Score = 125 bits (315), Expect = 4e-27
Identities = 60/104 (57%), Positives = 77/104 (74%), Gaps = 3/104 (2%)
Frame = +1
Query: 1 KMKTWGLTLLILLPTI---QEIIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEASYP 171
K + L+ LL + ++ A DGFI T+G+ F+LN PFY NGFNAYWLMY A+ P
Sbjct: 3 KHSAYAFLLIFLLQQVCLFVQVEAGDGFIRTRGIHFMLNGDPFYANGFNAYWLMYIAADP 62
Query: 172 SQRNKVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
SQR+KV++AF+EA +GLT+ARTWAFSD G RPLQ +PGSYNE+
Sbjct: 63 SQRSKVTDAFREASSHGLTVARTWAFSDGGYRPLQYAPGSYNED 106
>ref|XP_003528027.1| PREDICTED: mannan endo-1,4-beta-mannosidase 7-like [Glycine max]
Length = 420
Score = 125 bits (314), Expect = 6e-27
Identities = 59/101 (58%), Positives = 76/101 (75%), Gaps = 6/101 (5%)
Frame = +1
Query: 19 LTLLILLPTIQE------IIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEASYPSQR 180
L LL+L T+++ + A+ GF+ T+GVQ +LN P+Y NGFNAYWLMY AS PSQR
Sbjct: 9 LVLLVLYVTVEQSKLLHHVEADGGFVKTRGVQLMLNGSPYYANGFNAYWLMYLASDPSQR 68
Query: 181 NKVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
NK+S+ FQ+A +GL IARTWAFSD G +PLQ SPGSYN++
Sbjct: 69 NKISSVFQQASNHGLNIARTWAFSDGGYQPLQYSPGSYNDQ 109
>ref|XP_004512071.1| PREDICTED: mannan endo-1,4-beta-mannosidase 7-like [Cicer
arietinum]
Length = 424
Score = 125 bits (313), Expect = 8e-27
Identities = 60/95 (63%), Positives = 70/95 (73%)
Frame = +1
Query: 19 LTLLILLPTIQEIIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEASYPSQRNKVSNA 198
L LL +L + A DGF+ T+G+ F+LN P+Y NGFNAYWLMY AS PSQR KVSNA
Sbjct: 7 LFLLAILVHQGSVEAGDGFVRTRGIHFMLNGNPYYANGFNAYWLMYTASDPSQRYKVSNA 66
Query: 199 FQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
F EA +GLT+ARTWAFSD G PLQ SPG YNE+
Sbjct: 67 FHEATSHGLTVARTWAFSDGGYSPLQYSPGFYNEQ 101
>ref|XP_004143522.1| PREDICTED: mannan endo-1,4-beta-mannosidase 7-like [Cucumis
sativus] gi|449521449|ref|XP_004167742.1| PREDICTED:
mannan endo-1,4-beta-mannosidase 7-like [Cucumis
sativus]
Length = 418
Score = 125 bits (313), Expect = 8e-27
Identities = 61/100 (61%), Positives = 73/100 (73%)
Frame = +1
Query: 4 MKTWGLTLLILLPTIQEIIAEDGFITTKGVQFLLNEIPFYGNGFNAYWLMYEASYPSQRN 183
MK W L LL+ + I + DGF+ T +FLLN P+Y NGFNAYWLMY AS PSQR+
Sbjct: 1 MKLWVLALLLAI-FIHGVAGGDGFVRTGRTRFLLNGSPYYANGFNAYWLMYVASDPSQRS 59
Query: 184 KVSNAFQEALKNGLTIARTWAFSDAGSRPLQISPGSYNEE 303
KVS F+EA +GLT+ARTWAFSD G RPLQ SPG+YNE+
Sbjct: 60 KVSAVFREASSHGLTVARTWAFSDGGYRPLQYSPGNYNEQ 99