BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Achyranthes23_contig00024214
         (2088 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus ...   822   0.0  
gb|ADU15550.1| 4CL [Gossypium hirsutum]                               821   0.0  
gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum]          821   0.0  
gb|AFC89538.1| 4-coumarate: coenzyme A ligase 2 [Populus tomentosa]   818   0.0  
gb|EOY10077.1| 4-coumarate:CoA ligase 3 [Theobroma cacao]             818   0.0  
gb|ACZ06243.1| 4-coumarate:CoA ligase 2 [Gossypium hirsutum]          817   0.0  
ref|XP_002325815.1| 4-coumarate-CoA ligase family protein [Popul...   816   0.0  
gb|AAK58909.1| 4-coumarate:CoA ligase 4 [Populus trichocarpa x P...   811   0.0  
dbj|BAG82851.1| 4-coumarate:CoA ligase [Ipomoea batatas]              810   0.0  
gb|AAC24504.1| 4-coumarate:CoA ligase [Populus tremuloides]           809   0.0  
gb|AFG30056.1| 4-coumarate: CoA ligase [Malus hybrid cultivar]        808   0.0  
gb|ADE96997.1| p-coumarate:CoA-ligase 3 [Sorbus aucuparia]            807   0.0  
ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis ...   807   0.0  
gb|AEX32786.1| coumaroyl-CoA ligase [Vitis vinifera]                  806   0.0  
ref|XP_006443197.1| hypothetical protein CICLE_v10019532mg [Citr...   803   0.0  
gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]                  801   0.0  
gb|AGW16013.1| 4-coumarate:coenzyme A ligase 1 [Pueraria montana...   801   0.0  
sp|P31687.2|4CL2_SOYBN RecName: Full=4-coumarate--CoA ligase 2; ...   800   0.0  
ref|XP_004307864.1| PREDICTED: LOW QUALITY PROTEIN: 4-coumarate-...   796   0.0  
gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]        795   0.0  

>ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus communis]
            gi|223540792|gb|EEF42352.1| AMP dependent CoA ligase,
            putative [Ricinus communis]
          Length = 573

 Score =  822 bits (2123), Expect = 0.0
 Identities = 413/561 (73%), Positives = 476/561 (84%), Gaps = 7/561 (1%)
 Frame = +3

Query: 78   QPIDSEISSLKSPQK--PENLKTE-HIFKSKLPDIPITNHLPLHSYLFEKLPQVSEKPCL 248
            +P   E+SS  S QK  P +  +E HIF+SKLPDIPI+NHLPLH+Y FE L ++S+KPCL
Sbjct: 8    EPPKQELSSSSSSQKQNPSSSSSETHIFRSKLPDIPISNHLPLHTYCFENLFKLSDKPCL 67

Query: 249  ISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMIGAVT 428
            ISG+TGK YSFAETHL+ +KT AGLS LG+KKGDVIMILL NCPEFVF+F G SMIGAV 
Sbjct: 68   ISGSTGKTYSFAETHLISQKTAAGLSSLGIKKGDVIMILLQNCPEFVFSFMGASMIGAVA 127

Query: 429  TTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESE---EFQINQDFRVITIDNPPEN 599
            TTANPFYT+ EIFKQ+  S  K+I+TQS +VDKL++ +   + ++ +DF VITID PPEN
Sbjct: 128  TTANPFYTSNEIFKQLNSSRPKLIITQSLYVDKLRDPDHKIQPKLGEDFTVITIDEPPEN 187

Query: 600  CLHFSILSEANESDLPET-KINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQVDGE 776
            CLHF++LSEA+E+++PE    NPDDPV LPFSSGTTGLPKGVILTHKSL TSVAQQVDGE
Sbjct: 188  CLHFTVLSEASETEIPEVINANPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGE 247

Query: 777  NPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHRXXX 956
            NPN+ L EDDV+LCVLPLFHI+SLNSVL+ +LR G A+LLM KF+I +LLELIQKH+   
Sbjct: 248  NPNLYLKEDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFQIGALLELIQKHKVSV 307

Query: 957  XXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYGMTE 1136
                        KNPMV +FDLSSIR+V+SGAAPLGKELEDALR R+PQA+LGQGYGMTE
Sbjct: 308  AAVVPPLVLALAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTE 367

Query: 1137 AGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQIMK 1316
            AGPVLSMC  FAK+P  TK GSCG VVRNAE+K++DPETG SLGYN+ GEICIRG QIMK
Sbjct: 368  AGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGPQIMK 427

Query: 1317 EYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEALLV 1496
             YLNDP ATA TID+EGWLHTGDIGYVD+DDE+FIVDRVKEIIKFKGFQVPPAELEALL+
Sbjct: 428  GYLNDPEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAELEALLL 487

Query: 1497 NHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTVYFI 1676
            NHPSIADAAVVPQKDE AGEVPVAFVV+S NG +L+EE VK+YIAKQVVFYKKLH VYFI
Sbjct: 488  NHPSIADAAVVPQKDEVAGEVPVAFVVRS-NGFELSEEDVKEYIAKQVVFYKKLHKVYFI 546

Query: 1677 HAIPKSASGKILRKELRARLA 1739
            HAIPKS SGKILRK+L+A+LA
Sbjct: 547  HAIPKSPSGKILRKDLKAKLA 567


>gb|ADU15550.1| 4CL [Gossypium hirsutum]
          Length = 574

 Score =  821 bits (2121), Expect = 0.0
 Identities = 409/572 (71%), Positives = 482/572 (84%), Gaps = 12/572 (2%)
 Frame = +3

Query: 60   MAAVQSQPIDSEISSLKSPQKPENLKTE----HIFKSKLPDIPITNHLPLHSYLFEKLPQ 227
            M ++   P++ E      P KP+ L  E    HIF+SKLPDIPI+NHLPLH+Y FE LP 
Sbjct: 1    MISIADHPVELE------PPKPDQLSPETPADHIFRSKLPDIPISNHLPLHTYCFENLPS 54

Query: 228  VSEKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGT 407
            +S+KPCLISG++GKIYSF+ETHL+ +KT AGLSKLG++KGD IMILL NC EFVF F G 
Sbjct: 55   LSDKPCLISGSSGKIYSFSETHLIAQKTAAGLSKLGIQKGDAIMILLHNCAEFVFAFMGA 114

Query: 408  SMIGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKES-------EEF-QINQD 563
            SMIGAV+TTANPFYT+ EIFKQ K + AK+I+TQS++VDKLK++       E F +I +D
Sbjct: 115  SMIGAVSTTANPFYTSKEIFKQSKAARAKLIITQSQYVDKLKDTNNNNDGVENFPKIGED 174

Query: 564  FRVITIDNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSL 743
            F+VITID+PPENCL FS+LSEANE+++P+  I+PDDPV LPFSSGTTGLPKGV+LTHKSL
Sbjct: 175  FKVITIDDPPENCLDFSVLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSL 234

Query: 744  STSVAQQVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSL 923
             TSVAQQVDGENPN+ LT +DV+LCVLPLFHI+SLNSVL+ +LR G A+LLM KFEI +L
Sbjct: 235  ITSVAQQVDGENPNLYLTHEDVVLCVLPLFHIYSLNSVLLWSLRAGAAVLLMQKFEIGTL 294

Query: 924  LELIQKHRXXXXXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQ 1103
            LELIQ+H+               KNP+V +FDLSSIR+V+SGAAPLGKELEDALR R+PQ
Sbjct: 295  LELIQRHKVSVAAVVPPLVVLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEDALRGRVPQ 354

Query: 1104 AVLGQGYGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAG 1283
            AVLGQGYGMTEAGPVLSMC  FAK+P  TK GSCG VVRNAE+K++DPETG SLGYN+ G
Sbjct: 355  AVLGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPG 414

Query: 1284 EICIRGAQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQ 1463
            EICIRG+QIMK YLND  ATAATID+EGWLHTGDIGYVD DDE+FIVDRVKEIIKFKGFQ
Sbjct: 415  EICIRGSQIMKGYLNDGVATAATIDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQ 474

Query: 1464 VPPAELEALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVV 1643
            VPPAELE+LL++HPSIADAAVVPQKDE AGEVPVAFVV+S NG +LTEEAVK++IAKQVV
Sbjct: 475  VPPAELESLLISHPSIADAAVVPQKDEVAGEVPVAFVVRS-NGFELTEEAVKEFIAKQVV 533

Query: 1644 FYKKLHTVYFIHAIPKSASGKILRKELRARLA 1739
            +YK+LH V+F+HAIPKS SGKILRK+LRA+LA
Sbjct: 534  YYKRLHKVHFVHAIPKSPSGKILRKDLRAKLA 565


>gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum]
          Length = 573

 Score =  821 bits (2120), Expect = 0.0
 Identities = 407/567 (71%), Positives = 479/567 (84%), Gaps = 7/567 (1%)
 Frame = +3

Query: 60   MAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVSEK 239
            M ++   P+  E+ S K  Q       +HIF+SKLPDIPI+NHLPLH+Y FE LP +S+K
Sbjct: 1    MISIADHPV--ELESPKPDQLSPETPADHIFRSKLPDIPISNHLPLHTYCFENLPSLSDK 58

Query: 240  PCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMIG 419
            PCLISG++GKIYSF+ETHL+ +KT AGLS LG++KGD IMILL NC EFVF F G SMIG
Sbjct: 59   PCLISGSSGKIYSFSETHLIAQKTAAGLSNLGIQKGDAIMILLHNCAEFVFAFMGASMIG 118

Query: 420  AVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKES------EEF-QINQDFRVIT 578
            AV+TTANPFYT+ EIFKQ K + AK+I+TQS++VDKLK++      E F +I +DF+VIT
Sbjct: 119  AVSTTANPFYTSKEIFKQFKAARAKLIITQSQYVDKLKDTNNNDGDENFPKIGEDFKVIT 178

Query: 579  IDNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVA 758
            ID+PPENCL FS+LSEANE+++P+  I+PDDPV LPFSSGTTGLPKGV+LTHKSL TSVA
Sbjct: 179  IDDPPENCLDFSMLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITSVA 238

Query: 759  QQVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQ 938
            QQVDGENPN+ LT DDV+LCVLPLFHI+SLNSVL+ +LR G A+LLM KFEI +LLELIQ
Sbjct: 239  QQVDGENPNLYLTHDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELIQ 298

Query: 939  KHRXXXXXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQ 1118
            +H+               KNP+V +FDLSSIR+V+SGAAPLGKELEDALR R+PQAVLGQ
Sbjct: 299  RHKVSVAAVVPPLVVLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEDALRGRVPQAVLGQ 358

Query: 1119 GYGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIR 1298
            GYGMTEAGPVLSMC  FAK+P  TK GSCG VVRNAE+K++DPETG SLGYN+ GEICIR
Sbjct: 359  GYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIR 418

Query: 1299 GAQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAE 1478
            G+QIMK YLND  ATAATID+EGWLHTGDIGYVD DDE+FIVDRVKEIIKFKGFQVPPAE
Sbjct: 419  GSQIMKGYLNDGVATAATIDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQVPPAE 478

Query: 1479 LEALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKL 1658
            LE+LL++HPSIADAAVVPQKDE AGEVPVAFVV+S NG +LTEEAVK++IAKQVV+YK+L
Sbjct: 479  LESLLISHPSIADAAVVPQKDEVAGEVPVAFVVRS-NGFELTEEAVKEFIAKQVVYYKRL 537

Query: 1659 HTVYFIHAIPKSASGKILRKELRARLA 1739
            H V+F+HAIPKS SGKILRK+LRA+LA
Sbjct: 538  HKVHFVHAIPKSPSGKILRKDLRAKLA 564


>gb|AFC89538.1| 4-coumarate: coenzyme A ligase 2 [Populus tomentosa]
          Length = 569

 Score =  818 bits (2113), Expect = 0.0
 Identities = 414/566 (73%), Positives = 473/566 (83%), Gaps = 4/566 (0%)
 Frame = +3

Query: 54   MSMAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVS 233
            MS+A V+  P   E+S  ++   P + +T+HIF+SKLPDI I+NHLPLH+Y FE L   S
Sbjct: 1    MSVATVE--PPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENLSDFS 58

Query: 234  EKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSM 413
            ++PCLISG+TGK YSFAETHL+ RK  AGLS LG+KKGDVIM LL NCPEFVF+F G SM
Sbjct: 59   DRPCLISGSTGKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMGASM 118

Query: 414  IGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQINQ----DFRVITI 581
            IGAVTTTANPFYT  EIFKQ   S AK+I+TQS++V+KL +S+  + NQ    DF VITI
Sbjct: 119  IGAVTTTANPFYTQSEIFKQFSASRAKLIITQSQYVNKLGDSDCHENNQKPGEDFIVITI 178

Query: 582  DNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQ 761
            D+PPENC HF++L EANES++P   I PDDPV LPFSSGTTGLPKGVILTHKSL TSVAQ
Sbjct: 179  DDPPENCRHFNVLVEANESEMPTISILPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQ 238

Query: 762  QVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQK 941
            QVDGE PN+ L +DDV+LCVLPLFHIFSLNSVL+ +LR G A+LLM KFEI SLLELIQK
Sbjct: 239  QVDGEIPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQK 298

Query: 942  HRXXXXXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQG 1121
            H                KNP+V  FDLSSIR+V+SGAAPLGKELEDALR R+PQA+LGQG
Sbjct: 299  HNVSVAAVVPPLVLALAKNPLVANFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQG 358

Query: 1122 YGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRG 1301
            YGMTEAGPVLSMC AF+K+P  TK GSCG VVRNAE+K++DPETG SLGYN+ GEICIRG
Sbjct: 359  YGMTEAGPVLSMCLAFSKQPFPTKSGSCGTVVRNAELKVIDPETGGSLGYNQPGEICIRG 418

Query: 1302 AQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAEL 1481
            +QIMK YLND  ATA TID+EGWLHTGDIGYVD+DDE+FIVDRVKEIIKFKGFQVPPAEL
Sbjct: 419  SQIMKGYLNDAEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAEL 478

Query: 1482 EALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLH 1661
            EALLVNHPSIADAAVVPQKDE AGEVPVAFVV+S + LDL+EEAVKDYIAKQVVFYKKLH
Sbjct: 479  EALLVNHPSIADAAVVPQKDEVAGEVPVAFVVRS-DDLDLSEEAVKDYIAKQVVFYKKLH 537

Query: 1662 TVYFIHAIPKSASGKILRKELRARLA 1739
             V+F+H+IPKSASGKILRK+LRA+LA
Sbjct: 538  KVFFVHSIPKSASGKILRKDLRAKLA 563


>gb|EOY10077.1| 4-coumarate:CoA ligase 3 [Theobroma cacao]
          Length = 575

 Score =  818 bits (2112), Expect = 0.0
 Identities = 408/561 (72%), Positives = 479/561 (85%), Gaps = 7/561 (1%)
 Frame = +3

Query: 78   QPIDSEISSLKSPQ--KPENLKT-EHIFKSKLPDIPITNHLPLHSYLFEKLPQVSEKPCL 248
            +P   E+S   SP   KP +  T +HIF+SKLPDIPI+NHLPLH+Y FE L    +KPCL
Sbjct: 9    EPQKPELSPSLSPPATKPSSETTGDHIFRSKLPDIPISNHLPLHTYCFENLSSFPDKPCL 68

Query: 249  ISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMIGAVT 428
            ISG++GK YSF+ETHL+ +KT AGLS LG++KGDVIMILL NC EFVF+F G SMIGAV+
Sbjct: 69   ISGSSGKTYSFSETHLIAQKTAAGLSNLGIRKGDVIMILLQNCAEFVFSFMGASMIGAVS 128

Query: 429  TTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKES---EEF-QINQDFRVITIDNPPE 596
            TTANPFYT+ EIFKQ K++ AK+IVTQS++VDKLK++   E F ++ +DF+VITID+PPE
Sbjct: 129  TTANPFYTSTEIFKQYKVAQAKLIVTQSQYVDKLKDTSNNENFPKMGEDFKVITIDDPPE 188

Query: 597  NCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQVDGE 776
            NCLHF++LSEANE+++P+  I+PDDPV LPFSSGTTGLPKGV+LTHKSL TSVAQQVDGE
Sbjct: 189  NCLHFTVLSEANENEIPQVSIDPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGE 248

Query: 777  NPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHRXXX 956
            NPN+ L  DDV+LCVLPLFHI+SLNSVL+ ALR G A+LLM KFEI +LLELIQKH+   
Sbjct: 249  NPNLYLKHDDVVLCVLPLFHIYSLNSVLLCALRAGAAVLLMQKFEIGALLELIQKHKVSV 308

Query: 957  XXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYGMTE 1136
                        KNPMV ++DLSSIR+V+SGAAPLGKELE+ALR R+PQAVLGQGYGMTE
Sbjct: 309  AAVVPPLVLALAKNPMVAQYDLSSIRVVLSGAAPLGKELEEALRGRVPQAVLGQGYGMTE 368

Query: 1137 AGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQIMK 1316
            AGPVLSMC  FAK+P  TK GSCG VVRNAE+K++DPETG SLGYN+ GEICIRG+QIMK
Sbjct: 369  AGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGSQIMK 428

Query: 1317 EYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEALLV 1496
             YLND +ATA TID+EGWLHTGDIGYVD+ DE+FIVDRVKEIIKFKGFQVPPAELE+LLV
Sbjct: 429  GYLNDTSATATTIDVEGWLHTGDIGYVDDGDEIFIVDRVKEIIKFKGFQVPPAELESLLV 488

Query: 1497 NHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTVYFI 1676
            +HPSIADAAVVPQKDE AGEVPVAFVV+S NG +LTEEAVK++IAKQVVFYKKLH V+F+
Sbjct: 489  SHPSIADAAVVPQKDEVAGEVPVAFVVRS-NGFELTEEAVKEFIAKQVVFYKKLHKVHFV 547

Query: 1677 HAIPKSASGKILRKELRARLA 1739
            HAIPKS SGKILRK+LRA+LA
Sbjct: 548  HAIPKSPSGKILRKDLRAKLA 568


>gb|ACZ06243.1| 4-coumarate:CoA ligase 2 [Gossypium hirsutum]
          Length = 574

 Score =  817 bits (2111), Expect = 0.0
 Identities = 404/568 (71%), Positives = 479/568 (84%), Gaps = 8/568 (1%)
 Frame = +3

Query: 60   MAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVSEK 239
            M ++   P+  E+ S K  Q       +HIF+SKLPDIPI+NHLPLH+Y FE LP +S+K
Sbjct: 1    MISIADHPV--ELESPKPDQLSPETPADHIFRSKLPDIPISNHLPLHTYCFENLPSLSDK 58

Query: 240  PCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMIG 419
            PCLISG++GKIYSF+ETHL+ +KT AGLSKLG++KGD IMILL NC EFVF F G SMIG
Sbjct: 59   PCLISGSSGKIYSFSETHLIAQKTAAGLSKLGIQKGDAIMILLHNCAEFVFAFMGASMIG 118

Query: 420  AVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKES-------EEF-QINQDFRVI 575
            AV+TTANPFYT+ EIFKQ K + AK+I+TQS++VDKLK++       E F +I +DF+VI
Sbjct: 119  AVSTTANPFYTSKEIFKQFKAARAKLIITQSQYVDKLKDTNNNNDGVENFPKIGEDFKVI 178

Query: 576  TIDNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSV 755
            TID+PPENCL FS+LSEANE+++P+  I+PDDPV LPFSSGTTGLPKGV+LTHKSL TSV
Sbjct: 179  TIDDPPENCLDFSVLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITSV 238

Query: 756  AQQVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELI 935
            AQQVDGENPN+ LT +DV+LCVLPLFHI+SLNSVL+ +LR G A+LLM KFEI +LLELI
Sbjct: 239  AQQVDGENPNLYLTHEDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELI 298

Query: 936  QKHRXXXXXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLG 1115
            Q+H+               KNP+V +FDLSSIR+V+SGAAPLGKELE+ALR R+PQAVLG
Sbjct: 299  QRHKVSVAAVVPPLVLLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEEALRGRVPQAVLG 358

Query: 1116 QGYGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICI 1295
            QGYGMTEAGPVLSMC  FAK+P  TK GSCG VVRNAE+K++DPETG SLGYN+ GEICI
Sbjct: 359  QGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICI 418

Query: 1296 RGAQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPA 1475
            RG+QIMK YLND  ATAAT D+EGWLHTGDIGYVD DDE+FIVDRVKEIIKFKGFQVPPA
Sbjct: 419  RGSQIMKGYLNDSVATAATTDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQVPPA 478

Query: 1476 ELEALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKK 1655
            ELE+LL++HPSIADAAVVPQKDE AGEVPVAFVV+S NG +LTEEAVK++IA+QVV+YK+
Sbjct: 479  ELESLLISHPSIADAAVVPQKDEVAGEVPVAFVVRS-NGFELTEEAVKEFIARQVVYYKR 537

Query: 1656 LHTVYFIHAIPKSASGKILRKELRARLA 1739
            LH V+F+HAIPKS SGKILRK+LRA+LA
Sbjct: 538  LHKVHFVHAIPKSPSGKILRKDLRAKLA 565


>ref|XP_002325815.1| 4-coumarate-CoA ligase family protein [Populus trichocarpa]
            gi|118487236|gb|ABK95446.1| unknown [Populus trichocarpa]
            gi|222862690|gb|EEF00197.1| 4-coumarate-CoA ligase family
            protein [Populus trichocarpa]
          Length = 570

 Score =  816 bits (2109), Expect = 0.0
 Identities = 413/566 (72%), Positives = 472/566 (83%), Gaps = 4/566 (0%)
 Frame = +3

Query: 54   MSMAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVS 233
            MS+A V+  P   E+S  ++   P + +T+HIF+SKLPDI I+NHLPLH+Y FE L   S
Sbjct: 2    MSVATVE--PPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENLSDFS 59

Query: 234  EKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSM 413
            ++PCLISG+TGK YSFAETHL+ RK  AGLS LG+KKGDVIM LL NCPEFVF+F G SM
Sbjct: 60   DRPCLISGSTGKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMGASM 119

Query: 414  IGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQINQ----DFRVITI 581
            IGAVTTT NPFYT  EIFKQ   S AK+I+TQS+ V+KL++S+  + NQ    DF VITI
Sbjct: 120  IGAVTTTVNPFYTPGEIFKQFSASRAKLIITQSQHVNKLRDSDCHENNQKPEEDFIVITI 179

Query: 582  DNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQ 761
            D+PPENCLHF++L EANES++P   I+PDDPV LPFSSGTTGLPKGVILTHKSL TSVAQ
Sbjct: 180  DDPPENCLHFNVLVEANESEMPTVSIHPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQ 239

Query: 762  QVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQK 941
            QVDGE PN+ L +DDV+LCVLPLFHIFSLNSVL+ +LR G A+LLM KFEI SLLELIQK
Sbjct: 240  QVDGEIPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQK 299

Query: 942  HRXXXXXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQG 1121
            H                KNPMV  FDLSSIR+V+SGAAPLGKELE+ALR R+PQA+LGQG
Sbjct: 300  HNVSVAAVVPPLVLALAKNPMVANFDLSSIRVVLSGAAPLGKELEEALRSRVPQAILGQG 359

Query: 1122 YGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRG 1301
            YGMTEAGPVLSMC AF+K+PL TK GSCG VVRNAE+K++DPETG SLG N+ GEICIRG
Sbjct: 360  YGMTEAGPVLSMCLAFSKQPLPTKSGSCGTVVRNAELKVIDPETGSSLGRNQPGEICIRG 419

Query: 1302 AQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAEL 1481
            +QIMK YLND  ATA  ID+EGWLHTGDIGYVD+DDE+FIVDRVKEIIKFKGFQVPPAEL
Sbjct: 420  SQIMKGYLNDAEATANIIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAEL 479

Query: 1482 EALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLH 1661
            EALLVNHPSIADAAVVPQKDE AGEVPVAFVV+S N LDL EEAVKDYIAKQVVFYKKLH
Sbjct: 480  EALLVNHPSIADAAVVPQKDEVAGEVPVAFVVRS-NDLDLNEEAVKDYIAKQVVFYKKLH 538

Query: 1662 TVYFIHAIPKSASGKILRKELRARLA 1739
             V+F+H+IPKSA+GKILRK+LRA+LA
Sbjct: 539  KVFFVHSIPKSAAGKILRKDLRAKLA 564


>gb|AAK58909.1| 4-coumarate:CoA ligase 4 [Populus trichocarpa x Populus deltoides]
          Length = 579

 Score =  811 bits (2094), Expect = 0.0
 Identities = 414/575 (72%), Positives = 472/575 (82%), Gaps = 13/575 (2%)
 Frame = +3

Query: 54   MSMAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVS 233
            MS+A V+  P   E+S  ++   P + +T+HIF+SKLPDI I+NHLPLH+Y FE L   S
Sbjct: 2    MSVATVE--PPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENLSDFS 59

Query: 234  EKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSM 413
            ++PCLISG+TGK YSFAETHL+ RK  AGLS LG+KKGDVIM LL NCPEFVF+F G SM
Sbjct: 60   DRPCLISGSTGKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMGASM 119

Query: 414  IGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQINQ----DFRVITI 581
            IGAVTTT NPFYT  EIFKQ   S AK+I+TQS+ V+KL++S+  + NQ    DF VITI
Sbjct: 120  IGAVTTTVNPFYTPGEIFKQFSASRAKLIITQSQHVNKLRDSDYHENNQKPEEDFIVITI 179

Query: 582  DNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQ 761
            D+PPENCLHF++L EANES++P   I+PDDPV LPFSSGTTGLPKGVILTHKSL TSVAQ
Sbjct: 180  DDPPENCLHFNVLVEANESEMPTVSIHPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQ 239

Query: 762  QVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQK 941
            QVDGE PN+ L +DDV+LCVLPLFHIFSLNSVL+ +LR G A+LLM KFEI SLLELIQK
Sbjct: 240  QVDGEIPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQK 299

Query: 942  HRXXXXXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQG 1121
            H                KNPMV  FDLSSIR+V+SGAAPLGKELE+ALR R+PQA+LGQG
Sbjct: 300  HNVSVAAVVPPLVLALAKNPMVANFDLSSIRVVLSGAAPLGKELEEALRSRVPQAILGQG 359

Query: 1122 YGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRG 1301
            YGMTEAGPVLSMC AF+K+PL TK GSCG VVRNAE+K++DPETG SLG N+ GEICIRG
Sbjct: 360  YGMTEAGPVLSMCLAFSKQPLPTKSGSCGTVVRNAELKVIDPETGSSLGRNQPGEICIRG 419

Query: 1302 AQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAEL 1481
            +QIMK YLND  ATA  ID+EGWLHTGDIGYVD+DDE+FIVDRVKEIIKFKGFQVPPAEL
Sbjct: 420  SQIMKGYLNDAEATANIIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAEL 479

Query: 1482 EALLVNHPSIADAAVVP---------QKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAK 1634
            EALLVNHPSIADAAVVP         QKDE AGEVPVAFVV+S N LDL EEAVKDYIAK
Sbjct: 480  EALLVNHPSIADAAVVPRDNLYGNNRQKDEVAGEVPVAFVVRS-NDLDLNEEAVKDYIAK 538

Query: 1635 QVVFYKKLHTVYFIHAIPKSASGKILRKELRARLA 1739
            QVVFYKKLH V+F+H+IPKSASGKILRK+LRA+LA
Sbjct: 539  QVVFYKKLHKVFFVHSIPKSASGKILRKDLRAKLA 573


>dbj|BAG82851.1| 4-coumarate:CoA ligase [Ipomoea batatas]
          Length = 569

 Score =  810 bits (2091), Expect = 0.0
 Identities = 404/562 (71%), Positives = 473/562 (84%), Gaps = 3/562 (0%)
 Frame = +3

Query: 60   MAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVSEK 239
            M +V++Q  + E++     Q   +    HIF+S+LPDIPI N +PLH+Y F+ L +  ++
Sbjct: 1    MLSVETQKPELEVADSGKTQAQSS--QIHIFRSRLPDIPIPNQIPLHTYCFQNLAEYRDR 58

Query: 240  PCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMIG 419
             CLI G+TGK YSFAETHL+CRK  +GL+KLGVKKGDVIM L  NCPEFVF+F G SMIG
Sbjct: 59   TCLIVGSTGKTYSFAETHLICRKVASGLAKLGVKKGDVIMTLFQNCPEFVFSFMGASMIG 118

Query: 420  AVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKES-EEF-QINQDFRVITIDNPP 593
            AVTTTANPFYT  EIFKQ+  S AK+++TQS +VDKLK+S EE+ +I +DF V+TID+PP
Sbjct: 119  AVTTTANPFYTKAEIFKQMSASKAKVVITQSVYVDKLKDSGEEYPKIGEDFSVVTIDDPP 178

Query: 594  ENCLHFSILSEANESDLPET-KINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQVD 770
            ENCLHFS+LSEA+E D+P T +I PDD V LPFSSGTTGLPKGV+LTHKSL TSVAQQVD
Sbjct: 179  ENCLHFSVLSEADEEDMPATVEIAPDDAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVD 238

Query: 771  GENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHRX 950
            GENPN+ L EDDV+LCVLPLFHIFSLNSVL+ +LR G A+LLM KFEI SLLELI+KHR 
Sbjct: 239  GENPNLYLKEDDVVLCVLPLFHIFSLNSVLLCSLRAGAAVLLMQKFEIKSLLELIKKHRV 298

Query: 951  XXXXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYGM 1130
                          KNP+V+ +DLSSIR+V+SGAAPLGKELE+AL +R+PQA+ GQGYGM
Sbjct: 299  SVAAVVPPLVLALAKNPIVDSYDLSSIRVVLSGAAPLGKELEEALHQRVPQAIFGQGYGM 358

Query: 1131 TEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQI 1310
            TEAGPVLSMCPAFAK+ L  K GSCG VVRNAE+ +VDPETG SLG N+ GEICIRG+QI
Sbjct: 359  TEAGPVLSMCPAFAKQALPAKSGSCGSVVRNAELMVVDPETGCSLGRNQPGEICIRGSQI 418

Query: 1311 MKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEAL 1490
            MKEYLNDPAATA TID++GWLHTGDIGYVD+DDEVFIVDRVKE+IKFKGFQVPPAELEAL
Sbjct: 419  MKEYLNDPAATARTIDVDGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEAL 478

Query: 1491 LVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTVY 1670
            L++HP IADAAVVPQKD+AAGEVPVAFVV+S++G DLTEEAVK++IAKQVVFYKKLH VY
Sbjct: 479  LLSHPMIADAAVVPQKDDAAGEVPVAFVVRSSDGFDLTEEAVKEFIAKQVVFYKKLHKVY 538

Query: 1671 FIHAIPKSASGKILRKELRARL 1736
            FIHAIPKSASGKILRKELR +L
Sbjct: 539  FIHAIPKSASGKILRKELREKL 560


>gb|AAC24504.1| 4-coumarate:CoA ligase [Populus tremuloides]
          Length = 570

 Score =  809 bits (2090), Expect = 0.0
 Identities = 410/566 (72%), Positives = 471/566 (83%), Gaps = 4/566 (0%)
 Frame = +3

Query: 54   MSMAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVS 233
            MS+A V+  P   E+S  ++   P + +T+HIF+SKLPDI I+N LPLH+Y FE L   S
Sbjct: 2    MSVATVE--PPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNDLPLHAYCFENLSDFS 59

Query: 234  EKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSM 413
            ++PCLISG+TGK YSFAETHL+ RK  AGLS LG+KKGDVIM LL NCPEFVF+F G SM
Sbjct: 60   DRPCLISGSTGKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFIGASM 119

Query: 414  IGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQINQ----DFRVITI 581
            IGAV TTANPFYT  EIFKQ   S AK+I+TQS++V+KL +S+  + NQ    DF VITI
Sbjct: 120  IGAVITTANPFYTQSEIFKQFSASRAKLIITQSQYVNKLGDSDCHENNQKPGEDFIVITI 179

Query: 582  DNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQ 761
            D+PPENCLHF++L EA+ES++P   I PDDPV LPFSSGTTGLPKGVILTHKSL TSVAQ
Sbjct: 180  DDPPENCLHFNVLVEASESEMPTVSILPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQ 239

Query: 762  QVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQK 941
            QVDGE PN+ L +DDV+LCVLPLFHIFSLNSVL+ +LR G A+LLM KFEI SLLELIQK
Sbjct: 240  QVDGEIPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQK 299

Query: 942  HRXXXXXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQG 1121
            H                KNP+   FDLSSIR+V+SGAAPLGKELEDALR R+PQA+LGQG
Sbjct: 300  HNVSVAAVVPPLVLALAKNPLEANFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQG 359

Query: 1122 YGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRG 1301
            YGMTEAGPVLSMC AF+K+P  TK GSCG VVRNAE+K++DPETG SLGYN+ GEICIRG
Sbjct: 360  YGMTEAGPVLSMCLAFSKQPFPTKSGSCGTVVRNAELKVIDPETGRSLGYNQPGEICIRG 419

Query: 1302 AQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAEL 1481
            +QIMK YLND  ATA TID+EGWLHTGDIGYVD+DDE+FIVDRVKEIIKFKGFQVPPAEL
Sbjct: 420  SQIMKGYLNDAEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAEL 479

Query: 1482 EALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLH 1661
            EALLVNHPSIADAAVVPQKDE AGEVPVAFVV+S + LDL+EEAVK+YIAKQVVFYKKLH
Sbjct: 480  EALLVNHPSIADAAVVPQKDEVAGEVPVAFVVRS-DDLDLSEEAVKEYIAKQVVFYKKLH 538

Query: 1662 TVYFIHAIPKSASGKILRKELRARLA 1739
             V+F+H+IPKSASGKILRK+LRA+LA
Sbjct: 539  KVFFVHSIPKSASGKILRKDLRAKLA 564


>gb|AFG30056.1| 4-coumarate: CoA ligase [Malus hybrid cultivar]
          Length = 605

 Score =  808 bits (2088), Expect = 0.0
 Identities = 403/588 (68%), Positives = 482/588 (81%), Gaps = 10/588 (1%)
 Frame = +3

Query: 6    LSPSIIFFTTHHFQTNMSMAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITN 185
            L P +I  T+    T +   A  +  IDS  ++             H+F+SKLPDIPI N
Sbjct: 21   LMPPLINSTSQQNLTQLQPPACTNNIIDSTTATAT---------INHVFRSKLPDIPIPN 71

Query: 186  HLPLHSYLFEKLPQVSEKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMIL 365
            HLPLH+Y F+ LP+ S+KPCLI G+TGK YSF+ETHL+ +KTGAGLS LG++KGDVIMIL
Sbjct: 72   HLPLHTYCFQNLPEFSDKPCLIVGSTGKSYSFSETHLISQKTGAGLSNLGIQKGDVIMIL 131

Query: 366  LPNCPEFVFTFFGTSMIGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEE 545
            L NC EFVF F G S+IGAVTTTANPFYT+ EIFKQVK + AK+I+TQS++VDKL+E   
Sbjct: 132  LQNCAEFVFAFMGASLIGAVTTTANPFYTSAEIFKQVKAANAKLIITQSQYVDKLREHPS 191

Query: 546  F----------QINQDFRVITIDNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSS 695
                       ++ +DF+V+TID+PPENCLHFS+ SEANE +LP+  I+ +DPV LPFSS
Sbjct: 192  SADGADQNNYPKLGEDFKVVTIDDPPENCLHFSVFSEANEKELPDVVIDAEDPVALPFSS 251

Query: 696  GTTGLPKGVILTHKSLSTSVAQQVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALR 875
            GTTGLPKGVILTHKSL TSVAQQVDGENPN+ L EDDV+LCVLPLFHIFSLNSVL+ +LR
Sbjct: 252  GTTGLPKGVILTHKSLVTSVAQQVDGENPNLYLKEDDVVLCVLPLFHIFSLNSVLLCSLR 311

Query: 876  TGCAILLMPKFEINSLLELIQKHRXXXXXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAA 1055
             G  +LLM KFEI +LLELIQ++R               KNPMV +FDLSSIR+V+SGAA
Sbjct: 312  AGAGVLLMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKNPMVAEFDLSSIRVVLSGAA 371

Query: 1056 PLGKELEDALRERIPQAVLGQGYGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMK 1235
            PLGKELE+AL+ R+PQA+LGQGYGMTEAGPVLSMC AFAKEP+ TK GSCG VVRNAE+K
Sbjct: 372  PLGKELEEALKSRVPQALLGQGYGMTEAGPVLSMCMAFAKEPMPTKSGSCGTVVRNAELK 431

Query: 1236 IVDPETGVSLGYNEAGEICIRGAQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEV 1415
            ++D ETG+SLGYN++GEICIRG+QIMK YLND AATA T+D EGWLHTGD+GYVD+D+E+
Sbjct: 432  VLDLETGLSLGYNQSGEICIRGSQIMKGYLNDVAATATTVDTEGWLHTGDVGYVDDDNEI 491

Query: 1416 FIVDRVKEIIKFKGFQVPPAELEALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGL 1595
            FIVDR KE+IKFKGFQVPPAELE+LL++HPSIADAAVVPQKD+AAGEVPVAFVV+S NGL
Sbjct: 492  FIVDRAKELIKFKGFQVPPAELESLLISHPSIADAAVVPQKDDAAGEVPVAFVVRS-NGL 550

Query: 1596 DLTEEAVKDYIAKQVVFYKKLHTVYFIHAIPKSASGKILRKELRARLA 1739
            +LTEEAVK++IAKQVVFYK+LH V+F+HAIPKSASGKILRK+LRA+LA
Sbjct: 551  ELTEEAVKEFIAKQVVFYKRLHKVHFVHAIPKSASGKILRKDLRAKLA 598


>gb|ADE96997.1| p-coumarate:CoA-ligase 3 [Sorbus aucuparia]
          Length = 605

 Score =  807 bits (2085), Expect = 0.0
 Identities = 404/588 (68%), Positives = 481/588 (81%), Gaps = 10/588 (1%)
 Frame = +3

Query: 6    LSPSIIFFTTHHFQTNMSMAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITN 185
            L P +I  T+    T +   A  +  IDS  ++             H+F+SKLPDI I N
Sbjct: 21   LMPPLINSTSQQNLTKLQPPACTNNIIDSTTATAT---------INHVFRSKLPDIAIPN 71

Query: 186  HLPLHSYLFEKLPQVSEKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMIL 365
            HLPLH+Y F+ LP+ S++PCLI G+TGK YSF+ETHL+ +KTGAGLS LG++KGDVIMIL
Sbjct: 72   HLPLHTYCFQNLPEFSDRPCLIVGSTGKSYSFSETHLISQKTGAGLSNLGIQKGDVIMIL 131

Query: 366  LPNCPEFVFTFFGTSMIGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEE 545
            L NC EFVF F G S+IGAVTTTANPFYTT EIFKQVK + AK+I+TQS++V+KL+E   
Sbjct: 132  LQNCAEFVFAFMGASLIGAVTTTANPFYTTAEIFKQVKAANAKLIITQSQYVNKLREHPS 191

Query: 546  F----------QINQDFRVITIDNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSS 695
                       ++ +DF+V+TID+PPENCLHFS+LSEANE +LP+  I+ +DPV LPFSS
Sbjct: 192  SADGADQNNYPKLGEDFKVVTIDDPPENCLHFSVLSEANEKELPDVVIDAEDPVALPFSS 251

Query: 696  GTTGLPKGVILTHKSLSTSVAQQVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALR 875
            GTTGLPKGVILTHK+L TSVAQQVDGENPN+ L EDDV+LCVLPLFHIFS NSVL+ +LR
Sbjct: 252  GTTGLPKGVILTHKNLVTSVAQQVDGENPNLYLKEDDVVLCVLPLFHIFSFNSVLLCSLR 311

Query: 876  TGCAILLMPKFEINSLLELIQKHRXXXXXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAA 1055
             G  +LLM KFEI +LLELIQ++R               KNPMV +FDLSSIR+V+SGAA
Sbjct: 312  AGAGVLLMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKNPMVAEFDLSSIRVVLSGAA 371

Query: 1056 PLGKELEDALRERIPQAVLGQGYGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMK 1235
            PLGKELE+AL+ R+PQAVLGQGYGMTEAGPVLSMC AFAKEP+ TK GSCG VVRNAE+K
Sbjct: 372  PLGKELEEALKSRVPQAVLGQGYGMTEAGPVLSMCMAFAKEPMPTKSGSCGTVVRNAELK 431

Query: 1236 IVDPETGVSLGYNEAGEICIRGAQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEV 1415
            ++DPETG+SLGYN+ GEICIRG QIMK YLND AATA TID EGWLHTGD+GYVD+DDEV
Sbjct: 432  VLDPETGLSLGYNQPGEICIRGFQIMKGYLNDAAATATTIDTEGWLHTGDVGYVDDDDEV 491

Query: 1416 FIVDRVKEIIKFKGFQVPPAELEALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGL 1595
            FIVDRVKE+IKFKGFQVPPAELE+LL++HPSIADAAVVPQ+D+AAGEVPVAFVV+S +GL
Sbjct: 492  FIVDRVKELIKFKGFQVPPAELESLLISHPSIADAAVVPQRDDAAGEVPVAFVVRS-DGL 550

Query: 1596 DLTEEAVKDYIAKQVVFYKKLHTVYFIHAIPKSASGKILRKELRARLA 1739
            +LTEEAVK++IAKQVVFYK+LH V+F+HAIPKSASGKILRK+LRA+LA
Sbjct: 551  ELTEEAVKEFIAKQVVFYKRLHKVHFVHAIPKSASGKILRKDLRAKLA 598


>ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis vinifera]
          Length = 570

 Score =  807 bits (2085), Expect = 0.0
 Identities = 401/564 (71%), Positives = 474/564 (84%), Gaps = 4/564 (0%)
 Frame = +3

Query: 60   MAAVQSQPID-SEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVSE 236
            M ++++Q  D S + +  S  K  N   +H+F+SKLP+IPI+NHLPLH+Y FE   Q ++
Sbjct: 1    MISIETQNPDVSNLDTSHSIPKMANRIDDHVFRSKLPEIPISNHLPLHTYCFENYSQFAD 60

Query: 237  KPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMI 416
            +PCLI G+T K YSFAETHL+ RK GAG + LG+K+GDV+MILL NC EF F+F G SM+
Sbjct: 61   RPCLIVGSTNKTYSFAETHLISRKVGAGFAHLGLKQGDVVMILLQNCAEFAFSFLGASMV 120

Query: 417  GAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQ---INQDFRVITIDN 587
            GAVTTTANPFYT+ EIFKQ+  S AKI+VTQ+++VDKL++  + Q   I + F VITID+
Sbjct: 121  GAVTTTANPFYTSAEIFKQLNASKAKIVVTQAQYVDKLRDYPDGQVAKIGEGFTVITIDD 180

Query: 588  PPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQV 767
            PPENC+HFS++SEANES+LPE  IN DDPV LPFSSGTTGLPKGV+LTHKSL TSVAQQV
Sbjct: 181  PPENCMHFSVVSEANESELPEVSINSDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQV 240

Query: 768  DGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHR 947
            DGENPN++LT DDV+LCVLPLFHI+SLNSVL+ +LR G A+LLM KFEI +LLELIQ++R
Sbjct: 241  DGENPNLHLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELIQRYR 300

Query: 948  XXXXXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYG 1127
                           KNPMVE FDLSSIR+V+SGAAPLGKELE ALR R+PQAVLGQGYG
Sbjct: 301  VSVAAVVPPLVLALAKNPMVESFDLSSIRVVLSGAAPLGKELEAALRSRVPQAVLGQGYG 360

Query: 1128 MTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQ 1307
            MTEAGPVLSMC AFAK+P  TK GSCG VVRNAE+K+VDPETG SLG N+ GEICIRG Q
Sbjct: 361  MTEAGPVLSMCLAFAKQPFPTKSGSCGTVVRNAELKVVDPETGCSLGRNQPGEICIRGQQ 420

Query: 1308 IMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEA 1487
            IMK YLNDP ATA+TID++GWLHTGDIGYVD+D+EVFIVDRVKE+IKFKGFQVPPAELEA
Sbjct: 421  IMKGYLNDPEATASTIDVDGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEA 480

Query: 1488 LLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTV 1667
            LLV+HPSIADAAVVPQKD+ AGEVPVAFVV+S NG +LTEEAVK++I+KQVVFYK+LH V
Sbjct: 481  LLVSHPSIADAAVVPQKDDVAGEVPVAFVVRS-NGFELTEEAVKEFISKQVVFYKRLHKV 539

Query: 1668 YFIHAIPKSASGKILRKELRARLA 1739
            YF+HAIPKS SGKILRK+LRA+LA
Sbjct: 540  YFVHAIPKSPSGKILRKDLRAKLA 563


>gb|AEX32786.1| coumaroyl-CoA ligase [Vitis vinifera]
          Length = 570

 Score =  806 bits (2081), Expect = 0.0
 Identities = 400/564 (70%), Positives = 473/564 (83%), Gaps = 4/564 (0%)
 Frame = +3

Query: 60   MAAVQSQPID-SEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVSE 236
            M ++++Q  D S + +  S  K  N   +H+F+SKLP+IPI+NHLPLH+Y FE   Q ++
Sbjct: 1    MISIETQNPDVSNLDTSHSIPKMANRIDDHVFRSKLPEIPISNHLPLHTYCFENYSQFAD 60

Query: 237  KPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMI 416
            +PCLI G+T K YSFAETHL+ RK GAG + LG+K+GDV+MILL NC EF F+F G SM+
Sbjct: 61   RPCLIVGSTNKTYSFAETHLISRKVGAGFAHLGLKQGDVVMILLQNCAEFAFSFLGASMV 120

Query: 417  GAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQ---INQDFRVITIDN 587
            GAVTTTANPFYT+ EIFKQ+  S AKI+VTQ+++VDKL++  + Q   I + F VITID+
Sbjct: 121  GAVTTTANPFYTSAEIFKQLNASKAKIVVTQAQYVDKLRDYPDGQVAKIGEGFTVITIDD 180

Query: 588  PPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQV 767
            PPENC+HFS++SEANES+LPE  IN DDPV LPFSSGTTGLPKGV+LTHKSL TSVAQQV
Sbjct: 181  PPENCMHFSVVSEANESELPEVSINSDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQV 240

Query: 768  DGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHR 947
            DGENPN++LT DDV+LCVLPLFHI+SLNSVL+ +LR G A+LLM KFEI +LLELIQ++R
Sbjct: 241  DGENPNLHLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELIQRYR 300

Query: 948  XXXXXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYG 1127
                           KNPMVE FDLSSIR+V+SGAAPLGKELE ALR R+PQAVLGQGYG
Sbjct: 301  VSVAAVVPPLVLALAKNPMVESFDLSSIRVVLSGAAPLGKELEAALRSRVPQAVLGQGYG 360

Query: 1128 MTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQ 1307
            MTEAGPVLSMC  FAK+P  TK GSCG VVRNAE+K+VDPETG SLG N+ GEICIRG Q
Sbjct: 361  MTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVVDPETGCSLGRNQPGEICIRGQQ 420

Query: 1308 IMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEA 1487
            IMK YLNDP ATA+TID++GWLHTGDIGYVD+D+EVFIVDRVKE+IKFKGFQVPPAELEA
Sbjct: 421  IMKGYLNDPEATASTIDVDGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEA 480

Query: 1488 LLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTV 1667
            LLV+HPSIADAAVVPQKD+ AGEVPVAFVV+S NG +LTEEAVK++I+KQVVFYK+LH V
Sbjct: 481  LLVSHPSIADAAVVPQKDDVAGEVPVAFVVRS-NGFELTEEAVKEFISKQVVFYKRLHKV 539

Query: 1668 YFIHAIPKSASGKILRKELRARLA 1739
            YF+HAIPKS SGKILRK+LRA+LA
Sbjct: 540  YFVHAIPKSPSGKILRKDLRAKLA 563


>ref|XP_006443197.1| hypothetical protein CICLE_v10019532mg [Citrus clementina]
            gi|568850424|ref|XP_006478914.1| PREDICTED:
            4-coumarate--CoA ligase 2-like [Citrus sinensis]
            gi|557545459|gb|ESR56437.1| hypothetical protein
            CICLE_v10019532mg [Citrus clementina]
          Length = 560

 Score =  803 bits (2073), Expect = 0.0
 Identities = 402/563 (71%), Positives = 465/563 (82%), Gaps = 1/563 (0%)
 Frame = +3

Query: 54   MSMAAVQSQPIDSEISSLKSPQKPENLK-TEHIFKSKLPDIPITNHLPLHSYLFEKLPQV 230
            M   A +   +  +ISS  +P  P N K   HIFKSKLPDIPI+NHLPLH+Y F+   ++
Sbjct: 1    MISIATKKPELSLDISS-PAPPAPSNEKIATHIFKSKLPDIPISNHLPLHTYCFQD--RL 57

Query: 231  SEKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTS 410
            S+ PCLI G TGK YS+AETHL+CRKT AGLS LG+KKGDVIMILL NC EFVF+F G S
Sbjct: 58   SDDPCLIVGLTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGAS 117

Query: 411  MIGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQINQDFRVITIDNP 590
            MIGAVTTTANPFYT+ EI KQ + SGAK+I+T S++VD+L ++++     DF VITID P
Sbjct: 118  MIGAVTTTANPFYTSAEILKQFRTSGAKLIITMSQYVDRLPKTDK-----DFTVITIDAP 172

Query: 591  PENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQVD 770
            PENCLHF++LSEA+E  +PE  I PDDPV LPFSSGTTGLPKGV+LTHKSL TSVAQQVD
Sbjct: 173  PENCLHFTVLSEADEDQIPEVAIEPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVD 232

Query: 771  GENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHRX 950
            GENPN+ LT  DV+LCVLPLFHI+SLNSVL+ +LR G  +LLM KFEI +LLELIQ+HR 
Sbjct: 233  GENPNLYLTNGDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRV 292

Query: 951  XXXXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYGM 1130
                          KNPMV  +DLSSIR+V+SGAAPLGKELEDALR R+PQA+LGQGYGM
Sbjct: 293  SVAAVVPPLVLALAKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGM 352

Query: 1131 TEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQI 1310
            TEAGPVLSMC  FAK+P  TK GSCG VVRNAE+K++DPE G SL +N+ GEICIRG QI
Sbjct: 353  TEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPEIGASLPHNQPGEICIRGPQI 412

Query: 1311 MKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEAL 1490
            MK YLNDP ATAATID+EGWLHTGDIGYVD+DDEVFIVDRVKEIIKFKGFQVPPAE+EAL
Sbjct: 413  MKGYLNDPEATAATIDVEGWLHTGDIGYVDDDDEVFIVDRVKEIIKFKGFQVPPAEIEAL 472

Query: 1491 LVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTVY 1670
            L++HPSI DAAVVPQKDE AGEVPVAFVV+S NG +LTEEA+K+YIAKQVVFYK+LH +Y
Sbjct: 473  LLSHPSIGDAAVVPQKDEVAGEVPVAFVVRS-NGFELTEEAIKEYIAKQVVFYKRLHKIY 531

Query: 1671 FIHAIPKSASGKILRKELRARLA 1739
            F+HAIPKS SGKILRK+LRA+LA
Sbjct: 532  FVHAIPKSPSGKILRKDLRAKLA 554


>gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]
          Length = 604

 Score =  801 bits (2069), Expect = 0.0
 Identities = 394/544 (72%), Positives = 462/544 (84%), Gaps = 10/544 (1%)
 Frame = +3

Query: 138  TEHIFKSKLPDIPITNHLPLHSYLFEKLPQVSEKPCLISGATGKIYSFAETHLLCRKTGA 317
            T H+FKSKLPDIPI NHLPLH+Y F+KL + S++PCLI  +TGK YSF++T+L+ +KTGA
Sbjct: 56   TNHVFKSKLPDIPIPNHLPLHTYCFQKLSEFSDRPCLIVSSTGKSYSFSDTYLISQKTGA 115

Query: 318  GLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMIGAVTTTANPFYTTPEIFKQVKISGAKI 497
            GLS LG+KKGDVIMILL NC EFVF F G SMIGA+TTTANPFYT  EIFKQ K S AK+
Sbjct: 116  GLSNLGIKKGDVIMILLQNCAEFVFAFMGASMIGAITTTANPFYTATEIFKQFKNSNAKL 175

Query: 498  IVTQSKFVDKLKESEE----------FQINQDFRVITIDNPPENCLHFSILSEANESDLP 647
            I+T S+FVDKL+E+             ++ + F+V+T+D+PPENCLHFS++SEANE +LP
Sbjct: 176  IITHSQFVDKLREAPADGAAPDQNNYLKLGEHFKVVTVDDPPENCLHFSVISEANEKELP 235

Query: 648  ETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQVDGENPNMNLTEDDVLLCVLP 827
            E  I+P+DPV LPFSSGTTGLPKGVILTHKSL TSVAQQVDGENPN+ L EDDV+LCVLP
Sbjct: 236  EVVIDPEDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLP 295

Query: 828  LFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHRXXXXXXXXXXXXXXXKNPMV 1007
            LFHI+SLNSV++ +LR G A+L+M KFEI +LLELIQ++R               KNPMV
Sbjct: 296  LFHIYSLNSVMLCSLRAGAAVLVMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKNPMV 355

Query: 1008 EKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYGMTEAGPVLSMCPAFAKEPLT 1187
             +FDLSSIR+V+SGAAPLGKELE+ALR R+P AVLGQGYGMTEAGPVLSMC AFAKEPL 
Sbjct: 356  AQFDLSSIRVVLSGAAPLGKELEEALRSRVPGAVLGQGYGMTEAGPVLSMCLAFAKEPLP 415

Query: 1188 TKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQIMKEYLNDPAATAATIDIEG 1367
            +K GSCG V+RNAE+K++D ETG SLGYN+ GEICIRG+QIMK YLND  ATA T+D EG
Sbjct: 416  SKSGSCGTVIRNAELKVIDSETGCSLGYNQPGEICIRGSQIMKGYLNDAEATATTVDKEG 475

Query: 1368 WLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEALLVNHPSIADAAVVPQKDEA 1547
            WLHTGD+GYVD+D+EVFIVDRVKE+IKFKGFQVPPAELE+LLV+HPSIADAAVVPQKDEA
Sbjct: 476  WLHTGDVGYVDDDEEVFIVDRVKELIKFKGFQVPPAELESLLVSHPSIADAAVVPQKDEA 535

Query: 1548 AGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTVYFIHAIPKSASGKILRKELR 1727
            AGEVPVAFVV+S NGL LTEEAVK++IAKQVVFYK+LH VYF+HAIPKS SGKILRK+LR
Sbjct: 536  AGEVPVAFVVRS-NGLQLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLR 594

Query: 1728 ARLA 1739
            A+LA
Sbjct: 595  AKLA 598


>gb|AGW16013.1| 4-coumarate:coenzyme A ligase 1 [Pueraria montana var. lobata]
          Length = 567

 Score =  801 bits (2068), Expect = 0.0
 Identities = 395/549 (71%), Positives = 461/549 (83%), Gaps = 1/549 (0%)
 Frame = +3

Query: 93   EISSLKSPQKPE-NLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVSEKPCLISGATGK 269
            E+S+ K+ Q    + +T H+FKSKLPDIPI+NH+PLH+Y FEKL   +++PCLI GA  K
Sbjct: 12   EVSTPKTEQNQVCDPQTTHVFKSKLPDIPISNHIPLHAYCFEKLSDFADRPCLIVGAASK 71

Query: 270  IYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMIGAVTTTANPFY 449
             Y++AETHL+ RK  AGLS LG++KGDV+MILL N  EFVF+F   SMIGAV TTANPFY
Sbjct: 72   TYTYAETHLISRKIAAGLSNLGIRKGDVVMILLQNSAEFVFSFLAASMIGAVGTTANPFY 131

Query: 450  TTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQINQDFRVITIDNPPENCLHFSILSEA 629
            T  EIFKQ  +S AK+I+TQ+ +VDKL+  +   + +DF+V+T+D+PPENCLHFS+LSEA
Sbjct: 132  TAAEIFKQFTVSKAKLIITQAMYVDKLRHHDGTNLGEDFKVVTVDDPPENCLHFSVLSEA 191

Query: 630  NESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQVDGENPNMNLTEDDV 809
            NES++PE +I+PDD V +PFSSGTTGLPKGV+LTHKSL+TSVAQQVDGENPN+ LT +DV
Sbjct: 192  NESEVPEVEIHPDDAVAMPFSSGTTGLPKGVVLTHKSLTTSVAQQVDGENPNLYLTTEDV 251

Query: 810  LLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHRXXXXXXXXXXXXXX 989
            LLCVLPLFHIFSLNSVL+ ALR G  +LLM KFEI +LLELIQ+HR              
Sbjct: 252  LLCVLPLFHIFSLNSVLLCALRAGSGVLLMQKFEIGTLLELIQRHRVSVAMVVPPLVLAL 311

Query: 990  XKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYGMTEAGPVLSMCPAF 1169
             KNPMV  FDLSSIR+V+SGAAPLGKELE+ALR R+PQAVLGQGYGMTEAGPVLSMC  F
Sbjct: 312  AKNPMVADFDLSSIRLVLSGAAPLGKELEEALRNRVPQAVLGQGYGMTEAGPVLSMCLGF 371

Query: 1170 AKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQIMKEYLNDPAATAA 1349
            AK+P  TK GSCG VVRNAE+K+VDPETG SLGYN+ GEICIRG QIMK YLND  ATA 
Sbjct: 372  AKQPFPTKSGSCGTVVRNAELKVVDPETGRSLGYNQPGEICIRGQQIMKGYLNDEKATAL 431

Query: 1350 TIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEALLVNHPSIADAAVV 1529
            TID EGWLHTGD+GYVD+DDE+FIVDRVKE+IKFKGFQVPPAELE LLV+HPSIADAAVV
Sbjct: 432  TIDSEGWLHTGDVGYVDDDDEIFIVDRVKELIKFKGFQVPPAELEGLLVSHPSIADAAVV 491

Query: 1530 PQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTVYFIHAIPKSASGKI 1709
            PQKD AAGEVPVAFVV+S NG DLTEEAVK++IAKQVVFYK+LH VYF+HAIPKS SGKI
Sbjct: 492  PQKDVAAGEVPVAFVVRS-NGFDLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKI 550

Query: 1710 LRKELRARL 1736
            LRK+LRA+L
Sbjct: 551  LRKDLRAKL 559


>sp|P31687.2|4CL2_SOYBN RecName: Full=4-coumarate--CoA ligase 2; Short=4CL 2; AltName:
            Full=4-coumaroyl-CoA synthase 2; AltName: Full=Clone
            4CL16 gi|13559169|emb|CAC36095.1| 4-coumarate:Coenzyme A
            ligase isoenzyme 4 [Glycine max]
          Length = 562

 Score =  800 bits (2067), Expect = 0.0
 Identities = 395/549 (71%), Positives = 461/549 (83%), Gaps = 4/549 (0%)
 Frame = +3

Query: 102  SLKSPQKPENL----KTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVSEKPCLISGATGK 269
            SL +P+  +N     +T H+FKSKLPDIPI+NHLPLHSY F+ L Q + +PCLI G   K
Sbjct: 7    SLDTPKTDQNQVSDPQTSHVFKSKLPDIPISNHLPLHSYCFQNLSQFAHRPCLIVGPASK 66

Query: 270  IYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMIGAVTTTANPFY 449
             +++A+THL+  K  AGLS LG+ KGDV+MILL N  +FVF+F   SMIGAV TTANPFY
Sbjct: 67   TFTYADTHLISSKIAAGLSNLGILKGDVVMILLQNSADFVFSFLAISMIGAVATTANPFY 126

Query: 450  TTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQINQDFRVITIDNPPENCLHFSILSEA 629
            T PEIFKQ  +S AK+I+TQ+ +VDKL+  +  ++ +DF+V+T+D+PPENCLHFS+LSEA
Sbjct: 127  TAPEIFKQFTVSKAKLIITQAMYVDKLRNHDGAKLGEDFKVVTVDDPPENCLHFSVLSEA 186

Query: 630  NESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQVDGENPNMNLTEDDV 809
            NESD+PE +I+PDD V +PFSSGTTGLPKGVILTHKSL+TSVAQQVDGENPN+ LT +DV
Sbjct: 187  NESDVPEVEIHPDDAVAMPFSSGTTGLPKGVILTHKSLTTSVAQQVDGENPNLYLTTEDV 246

Query: 810  LLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHRXXXXXXXXXXXXXX 989
            LLCVLPLFHIFSLNSVL+ ALR G A+LLM KFEI +LLELIQ+HR              
Sbjct: 247  LLCVLPLFHIFSLNSVLLCALRAGSAVLLMQKFEIGTLLELIQRHRVSVAMVVPPLVLAL 306

Query: 990  XKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYGMTEAGPVLSMCPAF 1169
             KNPMV  FDLSSIR+V+SGAAPLGKELE+ALR R+PQAVLGQGYGMTEAGPVLSMC  F
Sbjct: 307  AKNPMVADFDLSSIRLVLSGAAPLGKELEEALRNRMPQAVLGQGYGMTEAGPVLSMCLGF 366

Query: 1170 AKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQIMKEYLNDPAATAA 1349
            AK+P  TK GSCG VVRNAE+K+VDPETG SLGYN+ GEICIRG QIMK YLND AATA+
Sbjct: 367  AKQPFQTKSGSCGTVVRNAELKVVDPETGRSLGYNQPGEICIRGQQIMKGYLNDEAATAS 426

Query: 1350 TIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEALLVNHPSIADAAVV 1529
            TID EGWLHTGD+GYVD+DDE+FIVDRVKE+IK+KGFQVPPAELE LLV+HPSIADAAVV
Sbjct: 427  TIDSEGWLHTGDVGYVDDDDEIFIVDRVKELIKYKGFQVPPAELEGLLVSHPSIADAAVV 486

Query: 1530 PQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTVYFIHAIPKSASGKI 1709
            PQKD AAGEVPVAFVV+S NG DLTEEAVK++IAKQVVFYK+LH VYF+HAIPKS SGKI
Sbjct: 487  PQKDVAAGEVPVAFVVRS-NGFDLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKI 545

Query: 1710 LRKELRARL 1736
            LRK+LRA+L
Sbjct: 546  LRKDLRAKL 554


>ref|XP_004307864.1| PREDICTED: LOW QUALITY PROTEIN: 4-coumarate--CoA ligase 2-like
            [Fragaria vesca subsp. vesca]
          Length = 586

 Score =  796 bits (2056), Expect = 0.0
 Identities = 404/575 (70%), Positives = 477/575 (82%), Gaps = 11/575 (1%)
 Frame = +3

Query: 48   TNMSMAAVQSQPIDSEISSLKSPQKPE-----NLKTEHIFKSKLPDIP-ITNHLPLHSYL 209
            TN +   V++  I   + S  SP+K        L   H+FKSKLPD+P I+NHLPLH+Y 
Sbjct: 8    TNNTSVVVETADISPTLISA-SPEKQHPATSTTLHDHHVFKSKLPDLPNISNHLPLHTYC 66

Query: 210  FEKLPQVSEKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFV 389
            FE +   SE+PCLI G+TGK YSF+ETHL+ +KTGAGLSKLG+ KGDVIMILL NC EFV
Sbjct: 67   FENVSDFSERPCLIVGSTGKTYSFSETHLISQKTGAGLSKLGITKGDVIMILLQNCAEFV 126

Query: 390  FTFFGTSMIGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLK----ESEEF-QI 554
            F F G SMIGAVTTTANPFYT  EIFKQ K S AK+I+TQS +VDKL+    +S+++ ++
Sbjct: 127  FAFMGASMIGAVTTTANPFYTASEIFKQFKASNAKLIITQSIYVDKLRHSGPDSDQYPKL 186

Query: 555  NQDFRVITIDNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTH 734
             + F+V+TID+PPENCLHFS+LS ANES+LPE  I+P+DPV LPFSSGTTGLPKGVILTH
Sbjct: 187  GEHFQVVTIDDPPENCLHFSVLSGANESELPEVSIDPEDPVALPFSSGTTGLPKGVILTH 246

Query: 735  KSLSTSVAQQVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEI 914
            KSL TSVAQQVDGENPN+ L EDDV+LCVLPLFHI+SLNSVL+ +LR G A+L+M KFEI
Sbjct: 247  KSLITSVAQQVDGENPNLYLKEDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLVMHKFEI 306

Query: 915  NSLLELIQKHRXXXXXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRER 1094
             +LLELIQ++R               +  MV  +DLSSIR+V+SGAAPLGKELE+ALR R
Sbjct: 307  GTLLELIQRYRVXCGSCGATAGDSAGEESMVADYDLSSIRVVLSGAAPLGKELEEALRSR 366

Query: 1095 IPQAVLGQGYGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYN 1274
            +PQAVLGQGYGMTEAGPVLSMC AFAK+P  TK GSCG VVRNAE+K+++PETG SLGYN
Sbjct: 367  VPQAVLGQGYGMTEAGPVLSMCLAFAKQPFPTKSGSCGTVVRNAELKVIEPETGRSLGYN 426

Query: 1275 EAGEICIRGAQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFK 1454
            + GEICIRG+QIMK YLND AATA T+D+EGWLHTGD+GYVD+D EVFIVDRVKE+IKFK
Sbjct: 427  QPGEICIRGSQIMKGYLNDDAATATTVDVEGWLHTGDVGYVDDDGEVFIVDRVKELIKFK 486

Query: 1455 GFQVPPAELEALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAK 1634
            GFQVPPAELE+LLV+H SIADAAVVPQKD+AAGEVPVAFVV+S NG +LTEEAVK++IAK
Sbjct: 487  GFQVPPAELESLLVSHQSIADAAVVPQKDDAAGEVPVAFVVRS-NGNELTEEAVKEFIAK 545

Query: 1635 QVVFYKKLHTVYFIHAIPKSASGKILRKELRARLA 1739
            QVVFYK+LH VYF+HAIPKS SGKILRK+LRA+LA
Sbjct: 546  QVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKLA 580


>gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]
          Length = 565

 Score =  795 bits (2054), Expect = 0.0
 Identities = 395/566 (69%), Positives = 477/566 (84%), Gaps = 4/566 (0%)
 Frame = +3

Query: 54   MSMAAVQSQPIDSEISSLKSPQK-PENLKTEH-IFKSKLPDIPITNHLPLHSYLFEKLPQ 227
            +S+A+ ++QP       + SP   PEN   E+ IF+SKLPDIPI+NHLPLH+Y FEK+ +
Sbjct: 2    LSVASPETQP------QIPSPTPTPENPPVENFIFRSKLPDIPISNHLPLHTYCFEKITE 55

Query: 228  VSEKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGT 407
             S+ PCLISG++G+IY+FAETHL+C+K+ AGLSKLG+KKGDVIMILL N PEF F+F G 
Sbjct: 56   FSDAPCLISGSSGRIYTFAETHLICQKSAAGLSKLGIKKGDVIMILLQNSPEFAFSFMGA 115

Query: 408  SMIGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKES-EEF-QINQDFRVITI 581
            SMIGA+TTTANPF T+ EIFKQ  +SGAK+++TQS++VDKL+++ E+F +I +DF VIT+
Sbjct: 116  SMIGAITTTANPFCTSAEIFKQFSVSGAKLVITQSQYVDKLRDTGEKFPKIGEDFTVITV 175

Query: 582  DNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQ 761
            D+PPE+CLHFS++S A+E ++P   I+PDDPV +PFSSGTTGLPKGV LTHKSL +SVAQ
Sbjct: 176  DDPPESCLHFSVISGADEREIPSVSIDPDDPVSMPFSSGTTGLPKGVFLTHKSLISSVAQ 235

Query: 762  QVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQK 941
            QVDGENPN+ L  DD++LCVLPLFHI+SLNSVL+ +LR G  +LLM KFEI SLLELIQ+
Sbjct: 236  QVDGENPNLYLKTDDIVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMHKFEIRSLLELIQR 295

Query: 942  HRXXXXXXXXXXXXXXXKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQG 1121
            HR               KNPMV  FDLSSIRIV+SGAAPLGKELE+AL  R+PQA+ GQG
Sbjct: 296  HRVSVAPVVPPLVLALAKNPMVASFDLSSIRIVLSGAAPLGKELEEALHSRLPQAIFGQG 355

Query: 1122 YGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRG 1301
            YGMTEAGPVLSMC  FA++   TK GSCG VVRNAE+K++DPETG SL YN+ GEICIRG
Sbjct: 356  YGMTEAGPVLSMCLGFARQAFPTKSGSCGTVVRNAELKVIDPETGFSLQYNQPGEICIRG 415

Query: 1302 AQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAEL 1481
             QIMK YLND  ATA+TID++GWLHTGD+GYVD+DDEVFIVDRVKE+IKFKGFQVPPAEL
Sbjct: 416  PQIMKGYLNDAEATASTIDVDGWLHTGDVGYVDDDDEVFIVDRVKELIKFKGFQVPPAEL 475

Query: 1482 EALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLH 1661
            EALL++HPSIADAAVVPQKDEAAGEVPVAFVV+S +G +LTEEAVK++IAKQVVFYK+LH
Sbjct: 476  EALLISHPSIADAAVVPQKDEAAGEVPVAFVVRS-SGFELTEEAVKEFIAKQVVFYKRLH 534

Query: 1662 TVYFIHAIPKSASGKILRKELRARLA 1739
             VYF+HAIPKSASGKIL K+LRA+LA
Sbjct: 535  KVYFVHAIPKSASGKILGKDLRAKLA 560