BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Achyranthes23_contig00017664
         (837 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAG84615.1| theobromine synthase [Camellia kissii]                 99   4e-40
dbj|BAG84616.1| theobromine synthase [Camellia lutchuensis]            99   1e-39
dbj|BAG84612.1| theobromine synthase [Camellia japonica]               99   1e-39
sp|Q9FZN8.1|TCS1_CAMSI RecName: Full=Caffeine synthase 1 gi|9967...    99   1e-39
dbj|BAE79732.1| theobromine synthase [Camellia ptilophylla]           100   1e-39
dbj|BAG84613.1| theobromine synthase [Camellia granthamiana]           99   1e-39
dbj|BAG84617.1| theobromine synthase [Camellia petelotii]              99   1e-39
gb|ABP98983.1| caffeine synthase [Camellia sinensis] gi|41101294...    99   2e-39
dbj|BAG84614.1| theobromine synthase [Camellia granthamiana]           99   2e-39
dbj|BAE79729.1| theobromine synthase [Camellia irrawadiensis]          99   9e-39
gb|AFZ93516.1| caffeine synthase 4 [Camellia sinensis]                 97   2e-37
gb|EOY17879.1| S-adenosyl-L-methionine-dependent methyltransfera...    98   8e-37
gb|AAW88351.1| caffeine synthase [Camellia sinensis]                   96   8e-37
sp|Q68CM3.1|TCS2_CAMSI RecName: Full=Probable caffeine synthase ...    96   4e-36
dbj|BAE79731.1| theobromine synthase [Camellia irrawadiensis]          96   6e-36
dbj|BAE79733.1| theobromine synthase [Camellia ptilophylla]            96   3e-35
gb|AFZ93517.1| caffeine synthase 5 [Camellia sinensis]                 89   5e-35
gb|EOY17880.1| S-adenosyl-L-methionine-dependent methyltransfera...    86   4e-34
gb|EOY17881.1| S-adenosyl-L-methionine-dependent methyltransfera...    86   4e-34
gb|EOY17887.1| S-adenosyl-L-methionine-dependent methyltransfera...    86   4e-34

>dbj|BAG84615.1| theobromine synthase [Camellia kissii]
          Length = 364

 Score = 98.6 bits (244), Expect(2) = 4e-40
 Identities = 46/75 (61%), Positives = 58/75 (77%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           SLH VH+SY++HW SQAPK L S +G  +NKG +YIS+TS  +V +AY++QF EDFT FL
Sbjct: 143 SLHLVHSSYSVHWLSQAPKGLRSREGLALNKGKIYISKTSPPVVREAYLSQFHEDFTMFL 202

Query: 187 ACRSQEVVPKGRMLL 231
             RSQEVVP G M+L
Sbjct: 203 NARSQEVVPNGCMVL 217



 Score = 94.0 bits (232), Expect(2) = 4e-40
 Identities = 56/130 (43%), Positives = 80/130 (61%), Gaps = 5/130 (3%)
 Frame = +3

Query: 240 GRPNS--SNSLTWQPWELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKK 413
           GR +S  SN  +   WEL     A+S L+ +GLI E+KLDTFN PYY  S E++K IV++
Sbjct: 221 GRKSSDPSNMESCFTWELLAI--AISELVSQGLIDEDKLDTFNVPYYTPSLEEMKDIVER 278

Query: 414 QGSFA---IESTKTIAHHVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDK 584
           +GSF    IE  +  + H++ +  DKW   +SLA+ IRAFTE +I + FG E++  +YDK
Sbjct: 279 EGSFTIDHIEGFELDSPHMQEK--DKWAGRESLAKAIRAFTEPIISNQFGHEIMDKLYDK 336

Query: 585 LAHCAFEYLD 614
             H     L+
Sbjct: 337 FTHIVVSDLE 346


>dbj|BAG84616.1| theobromine synthase [Camellia lutchuensis]
          Length = 366

 Score = 98.6 bits (244), Expect(2) = 1e-39
 Identities = 46/75 (61%), Positives = 58/75 (77%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           SLH VH+SY++HW SQAPK L S +G  +NKG +YIS+TS  +V +AY++QF EDFT FL
Sbjct: 145 SLHLVHSSYSVHWLSQAPKGLRSREGLALNKGKIYISKTSPPVVREAYLSQFHEDFTMFL 204

Query: 187 ACRSQEVVPKGRMLL 231
             RSQEVVP G M+L
Sbjct: 205 NARSQEVVPNGCMVL 219



 Score = 92.4 bits (228), Expect(2) = 1e-39
 Identities = 53/130 (40%), Positives = 78/130 (60%), Gaps = 5/130 (3%)
 Frame = +3

Query: 240 GRPNS--SNSLTWQPWELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKK 413
           GR +S  SN  +   WEL     A++ L+ +GLI E+KLDTFN PYY  S E++K IV++
Sbjct: 223 GRKSSDPSNMESCFTWELLAI--AIAGLVSQGLIDEDKLDTFNVPYYTPSLEEVKDIVER 280

Query: 414 QGSFAIESTKTIA---HHVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDK 584
           +GSF I+  + +     H++ +  DKW R   LA+ +RAFTE +I   FG E++  +YDK
Sbjct: 281 EGSFTIDHMEGLELDNPHMQEK--DKWVRGAKLAKAVRAFTEPIISKQFGHEIMNKLYDK 338

Query: 585 LAHCAFEYLD 614
             H     L+
Sbjct: 339 FTHIVVSELE 348


>dbj|BAG84612.1| theobromine synthase [Camellia japonica]
          Length = 365

 Score = 98.6 bits (244), Expect(2) = 1e-39
 Identities = 46/75 (61%), Positives = 58/75 (77%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           SLH VH+SY++HW SQAPK L S +G  +NKG +YIS+TS  +V +AY++QF EDFT FL
Sbjct: 144 SLHLVHSSYSVHWLSQAPKGLRSREGLALNKGKIYISKTSPPVVREAYLSQFHEDFTMFL 203

Query: 187 ACRSQEVVPKGRMLL 231
             RSQEVVP G M+L
Sbjct: 204 NARSQEVVPNGCMVL 218



 Score = 92.4 bits (228), Expect(2) = 1e-39
 Identities = 55/130 (42%), Positives = 79/130 (60%), Gaps = 5/130 (3%)
 Frame = +3

Query: 240 GRPNS--SNSLTWQPWELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKK 413
           GR +S  SN  +   WEL     A+S L+ +GLI E+KLDTFN PYY  S E++K IV++
Sbjct: 222 GRKSSDPSNMESCFTWELLAI--AISELVSQGLIDEDKLDTFNVPYYTPSLEEMKDIVER 279

Query: 414 QGSFA---IESTKTIAHHVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDK 584
           +GSF    IE  +  + H++ +  DKW   + LA+ IRAFTE +I + FG E++  +YDK
Sbjct: 280 EGSFTIDHIEGFELDSPHMQEK--DKWAGREKLAKAIRAFTEPIISNQFGHEIMDKLYDK 337

Query: 585 LAHCAFEYLD 614
             H     L+
Sbjct: 338 FTHIVVSDLE 347


>sp|Q9FZN8.1|TCS1_CAMSI RecName: Full=Caffeine synthase 1 gi|9967143|dbj|BAB12278.1|
           caffeine synthase [Camellia sinensis]
          Length = 369

 Score = 99.4 bits (246), Expect(2) = 1e-39
 Identities = 46/81 (56%), Positives = 60/81 (74%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           SLH VH+SY++HW +QAPK L S +G  +NKG +YIS+TS  +V +AY++QF EDFT FL
Sbjct: 148 SLHLVHSSYSVHWLTQAPKGLTSREGLALNKGKIYISKTSPPVVREAYLSQFHEDFTMFL 207

Query: 187 ACRSQEVVPKGRMLLTVEDAQ 249
             RSQEVVP G M+L +   Q
Sbjct: 208 NARSQEVVPNGCMVLILRGRQ 228



 Score = 91.3 bits (225), Expect(2) = 1e-39
 Identities = 52/127 (40%), Positives = 76/127 (59%), Gaps = 1/127 (0%)
 Frame = +3

Query: 279 WELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKKQGSFAIESTKTI-AH 455
           WEL     A++ L+ +GLI E+KLDTFN P Y AS E++K IV++ GSF I+  +     
Sbjct: 241 WEL--LAMAIAELVSQGLIDEDKLDTFNIPSYFASLEEVKDIVERDGSFTIDHIEGFDLD 298

Query: 456 HVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDKLAHCAFEYLDHGLFAMH 635
            VE + +DKW R +   +++RAFTE +I + FG E++  +YDK  H     L+  L    
Sbjct: 299 SVEMQENDKWVRGEKFTKVVRAFTEPIISNQFGPEIMDKLYDKFTHIVVSDLEAKL---P 355

Query: 636 HTVSILL 656
            T SI+L
Sbjct: 356 KTTSIIL 362


>dbj|BAE79732.1| theobromine synthase [Camellia ptilophylla]
          Length = 365

 Score =  100 bits (250), Expect(2) = 1e-39
 Identities = 47/81 (58%), Positives = 60/81 (74%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           SLH VH+SY++HW +QAPK L S +G  +NKG +YIS+TS  +V KAY++QF EDFT FL
Sbjct: 144 SLHLVHSSYSVHWLTQAPKGLTSREGLALNKGKIYISKTSPPVVKKAYLSQFHEDFTMFL 203

Query: 187 ACRSQEVVPKGRMLLTVEDAQ 249
             RSQEVVP G M+L +   Q
Sbjct: 204 NARSQEVVPNGCMVLILHGRQ 224



 Score = 89.7 bits (221), Expect(2) = 1e-39
 Identities = 52/140 (37%), Positives = 81/140 (57%), Gaps = 1/140 (0%)
 Frame = +3

Query: 240 GRPNSSNSLTWQPWELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKKQG 419
           GR +S  S     +  ++   A++ L+ +GLI ++KLDTFN P Y  S E++K IV++ G
Sbjct: 222 GRQSSDPSEMESCFTWELLAIAIAELVSQGLIDKDKLDTFNVPSYWPSLEEVKDIVERDG 281

Query: 420 SFAIESTKTIA-HHVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDKLAHC 596
           SF I+  +      +E + DDKW R    A+++RAFTE +I + FG+E++  +YDK  H 
Sbjct: 282 SFTIDHLEGFELDSLEMQEDDKWVRGDKFAKMVRAFTEPIISNQFGQEIMDKLYDKFTHI 341

Query: 597 AFEYLDHGLFAMHHTVSILL 656
               L+  L     T SI+L
Sbjct: 342 LVSDLEAEL---PKTTSIIL 358


>dbj|BAG84613.1| theobromine synthase [Camellia granthamiana]
          Length = 365

 Score = 98.6 bits (244), Expect(2) = 1e-39
 Identities = 46/75 (61%), Positives = 58/75 (77%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           SLH VH+SY++HW SQAPK L S +G  +NKG +YIS+TS  +V +AY++QF EDFT FL
Sbjct: 144 SLHLVHSSYSVHWLSQAPKGLRSREGLALNKGKIYISKTSPPVVREAYLSQFHEDFTMFL 203

Query: 187 ACRSQEVVPKGRMLL 231
             RSQEVVP G M+L
Sbjct: 204 NARSQEVVPNGCMVL 218



 Score = 92.0 bits (227), Expect(2) = 1e-39
 Identities = 54/130 (41%), Positives = 80/130 (61%), Gaps = 5/130 (3%)
 Frame = +3

Query: 240 GRPNS--SNSLTWQPWELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKK 413
           GR +S  SN  +   WEL     A+S L+ +GLI E+KLDTFN PYY  S E++K IV++
Sbjct: 222 GRKSSDPSNMESCFTWELLAI--AISELVSQGLIDEDKLDTFNVPYYTPSLEEMKDIVER 279

Query: 414 QGSFA---IESTKTIAHHVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDK 584
           +GSF    IE  +  + H++ +  DKW   + LA+ IRAFTE ++ + FG+E++  +YDK
Sbjct: 280 EGSFTIDHIEGFELDSPHMQEK--DKWAGREKLAKAIRAFTEPIMSNQFGDEIMDKLYDK 337

Query: 585 LAHCAFEYLD 614
             H     L+
Sbjct: 338 FTHIVVSDLE 347


>dbj|BAG84617.1| theobromine synthase [Camellia petelotii]
          Length = 355

 Score = 98.6 bits (244), Expect(2) = 1e-39
 Identities = 46/75 (61%), Positives = 58/75 (77%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           SLH VH+SY++HW SQAPK L S +G  +NKG +YIS+TS  +V +AY++QF EDFT FL
Sbjct: 134 SLHLVHSSYSVHWLSQAPKGLRSREGLALNKGKIYISKTSPPVVREAYLSQFHEDFTMFL 193

Query: 187 ACRSQEVVPKGRMLL 231
             RSQEVVP G M+L
Sbjct: 194 NARSQEVVPNGCMVL 208



 Score = 92.0 bits (227), Expect(2) = 1e-39
 Identities = 54/130 (41%), Positives = 79/130 (60%), Gaps = 5/130 (3%)
 Frame = +3

Query: 240 GRPNS--SNSLTWQPWELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKK 413
           GR +S  SN  +   WEL     A++ L+ +GLI E+KLDTFN PYY  S E++K IV++
Sbjct: 212 GRKSSDPSNMESCFTWELLAI--AIAELVSQGLIDEDKLDTFNVPYYTPSLEEMKDIVER 269

Query: 414 QGSFA---IESTKTIAHHVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDK 584
           +GSF    IE  +  + H++ +  DKW   + LA+ IRAFTE +I + FG E++  +YDK
Sbjct: 270 EGSFTIDHIEGVELDSPHMQEK--DKWAGREKLAKAIRAFTEPIISNQFGHEIMDKLYDK 327

Query: 585 LAHCAFEYLD 614
             H     L+
Sbjct: 328 FTHIVVSDLE 337


>gb|ABP98983.1| caffeine synthase [Camellia sinensis] gi|411012944|gb|AFV99128.1|
           caffeine synthase 1 [Camellia sinensis]
          Length = 369

 Score = 99.4 bits (246), Expect(2) = 2e-39
 Identities = 46/81 (56%), Positives = 60/81 (74%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           SLH VH+SY++HW +QAPK L S +G  +NKG +YIS+TS  +V +AY++QF EDFT FL
Sbjct: 148 SLHLVHSSYSVHWLTQAPKGLTSREGLALNKGKIYISKTSPPVVREAYLSQFHEDFTMFL 207

Query: 187 ACRSQEVVPKGRMLLTVEDAQ 249
             RSQEVVP G M+L +   Q
Sbjct: 208 NARSQEVVPNGCMVLILRGRQ 228



 Score = 90.5 bits (223), Expect(2) = 2e-39
 Identities = 51/127 (40%), Positives = 76/127 (59%), Gaps = 1/127 (0%)
 Frame = +3

Query: 279 WELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKKQGSFAIESTKTI-AH 455
           WEL     A++ L+ +GLI E+KLDTFN P Y AS E++K IV++ GSF I+  +     
Sbjct: 241 WEL--LAMAIAELVSQGLIDEDKLDTFNIPSYFASLEEVKDIVERDGSFTIDHIEGFDLD 298

Query: 456 HVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDKLAHCAFEYLDHGLFAMH 635
            +E + +DKW R +   +++RAFTE +I + FG E++  +YDK  H     L+  L    
Sbjct: 299 SLEMQENDKWVRGEKFTKVVRAFTEPIISNQFGHEIMDKLYDKFTHIVVSDLEAKL---P 355

Query: 636 HTVSILL 656
            T SI+L
Sbjct: 356 KTTSIIL 362


>dbj|BAG84614.1| theobromine synthase [Camellia granthamiana]
          Length = 366

 Score = 98.6 bits (244), Expect(2) = 2e-39
 Identities = 46/75 (61%), Positives = 58/75 (77%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           SLH VH+SY++HW SQAPK L S +G  +NKG +YIS+TS  +V +AY++QF EDFT FL
Sbjct: 145 SLHLVHSSYSVHWLSQAPKGLRSREGLALNKGKIYISKTSPPVVREAYLSQFHEDFTMFL 204

Query: 187 ACRSQEVVPKGRMLL 231
             RSQEVVP G M+L
Sbjct: 205 NARSQEVVPNGCMVL 219



 Score = 91.3 bits (225), Expect(2) = 2e-39
 Identities = 47/113 (41%), Positives = 71/113 (62%), Gaps = 1/113 (0%)
 Frame = +3

Query: 279 WELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKKQGSFAIESTKTI-AH 455
           WEL     A++ L+ +GLI E+KLDTFN PYY  S E++K IV+++GSF I+  + +   
Sbjct: 238 WELLAI--AIAELVSQGLIDEDKLDTFNVPYYTPSLEEVKDIVEREGSFTIDHMEGLELD 295

Query: 456 HVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDKLAHCAFEYLD 614
             + +  DKW R + LA+ +RAFTE +I + FG E++  +YDK  H     L+
Sbjct: 296 SPQMQEKDKWVRGEKLAKAVRAFTEPIISNQFGHEIMDKLYDKFTHIVVSDLE 348


>dbj|BAE79729.1| theobromine synthase [Camellia irrawadiensis]
          Length = 365

 Score = 99.4 bits (246), Expect(2) = 9e-39
 Identities = 46/81 (56%), Positives = 60/81 (74%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           SLH VH+SY++HW +QAPK L S +G  +NKG +YIS+TS  +V +AY++QF EDFT FL
Sbjct: 144 SLHLVHSSYSVHWLTQAPKGLTSREGLALNKGKIYISKTSPPVVKEAYLSQFHEDFTMFL 203

Query: 187 ACRSQEVVPKGRMLLTVEDAQ 249
             RSQEVVP G M+L +   Q
Sbjct: 204 NARSQEVVPNGCMVLILHGRQ 224



 Score = 88.6 bits (218), Expect(2) = 9e-39
 Identities = 52/140 (37%), Positives = 80/140 (57%), Gaps = 1/140 (0%)
 Frame = +3

Query: 240 GRPNSSNSLTWQPWELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKKQG 419
           GR +S  S     +  ++   A++ L+ +GLI E+KLDTFN P Y  S E++K IV++ G
Sbjct: 222 GRQSSDPSEMESCFTWELLAIAIAELVSQGLIDEDKLDTFNVPSYWPSLEEVKDIVERDG 281

Query: 420 SFAIESTKTI-AHHVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDKLAHC 596
           SF I+  +      +E + +DKW R    A+++RAFTE +I + FG E++  +YDK  H 
Sbjct: 282 SFTIDHLEGFELDSLEMQENDKWVRGDKFAKMVRAFTEPIISNQFGHEIMDKLYDKFTHI 341

Query: 597 AFEYLDHGLFAMHHTVSILL 656
               L+  L     T SI+L
Sbjct: 342 LVSDLEAEL---PKTTSIIL 358


>gb|AFZ93516.1| caffeine synthase 4 [Camellia sinensis]
          Length = 365

 Score = 97.1 bits (240), Expect(2) = 2e-37
 Identities = 45/81 (55%), Positives = 58/81 (71%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           SLH VH+ Y+ HW SQAPK L S +G P+NKG +YIS+ S  +V +AY++QF +DFT FL
Sbjct: 144 SLHLVHSCYSAHWLSQAPKGLTSREGLPLNKGKIYISKRSPPVVREAYLSQFHDDFTMFL 203

Query: 187 ACRSQEVVPKGRMLLTVEDAQ 249
             RSQEVVP G M+L +   Q
Sbjct: 204 NARSQEVVPHGCMVLILPSRQ 224



 Score = 86.7 bits (213), Expect(2) = 2e-37
 Identities = 45/106 (42%), Positives = 68/106 (64%), Gaps = 1/106 (0%)
 Frame = +3

Query: 279 WELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKKQGSFAIESTKTI-AH 455
           WEL     A++ L+ +GLI E+KLDTFN P Y  S E++K IV++ GSF I+  +     
Sbjct: 237 WELLAI--AIAELVSQGLIDEDKLDTFNVPSYFPSLEEVKDIVERDGSFTIDHMEGFELD 294

Query: 456 HVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDKLAH 593
            ++ + +DKW R + LA+ +RAFTE +I + FG E++  +YDK  H
Sbjct: 295 TLQMQENDKWIRGEKLAKAVRAFTEPIISNQFGHEIMDKLYDKFTH 340


>gb|EOY17879.1| S-adenosyl-L-methionine-dependent methyltransferases superfamily
           protein [Theobroma cacao]
          Length = 689

 Score = 98.2 bits (243), Expect(2) = 8e-37
 Identities = 45/77 (58%), Positives = 60/77 (77%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           S+H VH+SY +HW S+APK  C E+G P+NKG+++ISETS   V KAY++QF+EDF  FL
Sbjct: 472 SMHLVHSSYGIHWLSKAPKITC-ERGLPLNKGSIHISETSPLKVRKAYLSQFQEDFLLFL 530

Query: 187 ACRSQEVVPKGRMLLTV 237
            CRS E+VP GR+L T+
Sbjct: 531 KCRSPEMVPNGRILFTI 547



 Score = 83.2 bits (204), Expect(2) = 8e-37
 Identities = 42/122 (34%), Positives = 75/122 (61%)
 Frame = +3

Query: 240 GRPNSSNSLTWQPWELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKKQG 419
           G P    SL    +  ++ ++A+S ++ +GLI ++KLD+ N P +  S+E+++ +V+K+G
Sbjct: 553 GDPIGKESL----YNFELLSRAISYMVSKGLIDKDKLDSLNAPTFTPSQEEVEDLVEKEG 608

Query: 420 SFAIESTKTIAHHVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDKLAHCA 599
           SF +E   TI    + E+ D W  ++S A+ +RAF+E  I H FGEE++  +Y+K+    
Sbjct: 609 SFTVEFIDTI----KVEVGDAWASAESRAKNVRAFSEPWISHQFGEEIMDKLYEKVTQIV 664

Query: 600 FE 605
            E
Sbjct: 665 VE 666



 Score = 89.0 bits (219), Expect(2) = 5e-32
 Identities = 41/81 (50%), Positives = 59/81 (72%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           S+H VH+ Y++HW S+APK + SE+G P+NKG +Y+S+TS   V +AY++QFEEDF+  L
Sbjct: 142 SMHLVHSCYSVHWLSKAPK-ITSEEGLPLNKGKIYMSKTSPPAVKEAYLSQFEEDFSSVL 200

Query: 187 ACRSQEVVPKGRMLLTVEDAQ 249
             RS E+ P GRM+L +   Q
Sbjct: 201 RFRSPELAPDGRMVLILNGRQ 221



 Score = 76.3 bits (186), Expect(2) = 5e-32
 Identities = 37/98 (37%), Positives = 61/98 (62%)
 Frame = +3

Query: 291 IFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKKQGSFAIESTKTIAHHVENE 470
           +  +ALS L+ EGLI EEKL +FN PYY  S+E++++++ K+GSF  E + T+   +  +
Sbjct: 236 LLAEALSYLVSEGLIDEEKLGSFNVPYYNPSQEEVERVIDKEGSFTTEFSDTVVLEIGGK 295

Query: 471 IDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDK 584
             + W       +  R F+E ++ H FGEEV+  ++DK
Sbjct: 296 --NAWSDPGLRIKGYRCFSEPVLSHQFGEEVMDKLFDK 331


>gb|AAW88351.1| caffeine synthase [Camellia sinensis]
          Length = 365

 Score = 96.3 bits (238), Expect(2) = 8e-37
 Identities = 44/75 (58%), Positives = 58/75 (77%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           SLH VH+ Y++HW +QAPK L S++G  +NKG +YIS+TS  +V +AY++QF EDFT FL
Sbjct: 144 SLHLVHSCYSVHWLTQAPKGLTSKEGLALNKGKIYISKTSPPVVREAYLSQFHEDFTMFL 203

Query: 187 ACRSQEVVPKGRMLL 231
             RSQEVVP G M+L
Sbjct: 204 NSRSQEVVPNGCMVL 218



 Score = 85.1 bits (209), Expect(2) = 8e-37
 Identities = 46/113 (40%), Positives = 67/113 (59%), Gaps = 1/113 (0%)
 Frame = +3

Query: 279 WELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKKQGSFAIESTKTI-AH 455
           WEL     A++ L+ +GLI E+KLDTFN P Y  S E++K IV++ GSF I+  +     
Sbjct: 237 WEL--LAVAIAELVSQGLIDEDKLDTFNVPSYFPSLEEVKDIVERNGSFTIDHMEGFELD 294

Query: 456 HVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDKLAHCAFEYLD 614
             E + DDKW R +  A + RAFTE +I + FG E++  +Y+K  H     L+
Sbjct: 295 SPEMQEDDKWVRGEKFATVARAFTEPIISNQFGHEIMDKLYEKFTHIVVSDLE 347


>sp|Q68CM3.1|TCS2_CAMSI RecName: Full=Probable caffeine synthase 2
           gi|51968288|dbj|BAD42854.1| caffeine synthase [Camellia
           sinensis] gi|411012946|gb|AFV99129.1| caffeine synthase
           2 [Camellia sinensis]
          Length = 365

 Score = 96.3 bits (238), Expect(2) = 4e-36
 Identities = 44/75 (58%), Positives = 58/75 (77%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           SLH VH+ Y++HW +QAPK L S++G  +NKG +YIS+TS  +V +AY++QF EDFT FL
Sbjct: 144 SLHLVHSCYSVHWLTQAPKGLTSKEGLALNKGKIYISKTSPPVVREAYLSQFHEDFTMFL 203

Query: 187 ACRSQEVVPKGRMLL 231
             RSQEVVP G M+L
Sbjct: 204 NSRSQEVVPNGCMVL 218



 Score = 82.8 bits (203), Expect(2) = 4e-36
 Identities = 44/106 (41%), Positives = 65/106 (61%), Gaps = 1/106 (0%)
 Frame = +3

Query: 279 WELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKKQGSFAIESTKTI-AH 455
           WEL     A++ L+ +GLI E+KLDTFN P Y  S E++K IV++ GSF I+  +     
Sbjct: 237 WEL--LAVAIAELVSQGLIDEDKLDTFNVPSYFPSLEEVKDIVERNGSFTIDHMEGFELD 294

Query: 456 HVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDKLAH 593
             E + +DKW R +  A + RAFTE +I + FG E++  +Y+K  H
Sbjct: 295 SPEMQENDKWVRGEKFATVARAFTEPIISNQFGHEIMDKLYEKFTH 340


>dbj|BAE79731.1| theobromine synthase [Camellia irrawadiensis]
          Length = 364

 Score = 96.3 bits (238), Expect(2) = 6e-36
 Identities = 44/75 (58%), Positives = 58/75 (77%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           SLH VH+ Y++HW +QAPK L S++G  +NKG +YIS+TS  +V +AY++QF EDFT FL
Sbjct: 143 SLHLVHSCYSVHWLTQAPKGLTSKEGLALNKGKIYISKTSPPVVREAYLSQFHEDFTMFL 202

Query: 187 ACRSQEVVPKGRMLL 231
             RSQEVVP G M+L
Sbjct: 203 NSRSQEVVPNGCMVL 217



 Score = 82.0 bits (201), Expect(2) = 6e-36
 Identities = 44/113 (38%), Positives = 67/113 (59%), Gaps = 1/113 (0%)
 Frame = +3

Query: 279 WELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKKQGSFAIESTKTI-AH 455
           WEL     A++ L+ +GLI E+KLDTFN P Y  S E++K IV++ GSF I+  +     
Sbjct: 236 WEL--LAVAIAELVSQGLIDEDKLDTFNVPSYFPSLEEVKDIVERNGSFTIDHMEGFELD 293

Query: 456 HVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDKLAHCAFEYLD 614
             + + +DKW R +  A + RAFTE +I + FG E++  +Y+K  H     L+
Sbjct: 294 SPQMQENDKWVRGEKFATVARAFTEPIISNQFGHEIMDKLYEKFTHIVVSDLE 346


>dbj|BAE79733.1| theobromine synthase [Camellia ptilophylla]
          Length = 365

 Score = 96.3 bits (238), Expect(2) = 3e-35
 Identities = 44/75 (58%), Positives = 58/75 (77%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           SLH VH+ Y++HW +QAPK L S++G  +NKG +YIS+TS  +V +AY++QF EDFT FL
Sbjct: 144 SLHLVHSCYSVHWLTQAPKGLTSKEGLALNKGKIYISKTSPPVVREAYLSQFHEDFTMFL 203

Query: 187 ACRSQEVVPKGRMLL 231
             RSQEVVP G M+L
Sbjct: 204 NSRSQEVVPNGCMVL 218



 Score = 79.7 bits (195), Expect(2) = 3e-35
 Identities = 43/113 (38%), Positives = 66/113 (58%), Gaps = 1/113 (0%)
 Frame = +3

Query: 279 WELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKKQGSFAIESTKTI-AH 455
           WEL     A++ L+ +GLI E+KLDTFN P Y  S E++K IV++ GSF I+  +     
Sbjct: 237 WEL--LAAAIAELVSQGLIDEDKLDTFNVPSYFPSLEEVKDIVERNGSFTIDHMEGFELD 294

Query: 456 HVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDKLAHCAFEYLD 614
             + + +DKW R +  A + RA TE +I + FG E++  +Y+K  H     L+
Sbjct: 295 SPQMQENDKWVRGEKFATVARASTEPIISNQFGHEIMDKLYEKFTHIVISDLE 347


>gb|AFZ93517.1| caffeine synthase 5 [Camellia sinensis]
          Length = 365

 Score = 89.4 bits (220), Expect(2) = 5e-35
 Identities = 43/81 (53%), Positives = 56/81 (69%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           SL  VH+ Y+ HW SQAPK L S +G  +N+  +YIS+TS  +V +AY++QF EDFT FL
Sbjct: 144 SLRLVHSCYSAHWLSQAPKGLTSREGLALNRRKIYISKTSPLVVREAYLSQFHEDFTMFL 203

Query: 187 ACRSQEVVPKGRMLLTVEDAQ 249
             RSQEVVP G M+L +   Q
Sbjct: 204 NARSQEVVPNGCMVLILPGRQ 224



 Score = 85.9 bits (211), Expect(2) = 5e-35
 Identities = 51/132 (38%), Positives = 75/132 (56%), Gaps = 7/132 (5%)
 Frame = +3

Query: 240 GRPNSSNSLTWQPWELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKKQG 419
           GR +S+ S     +  ++   A+  L+ +GLI E+KLDTFN P Y  S E++K IV++ G
Sbjct: 222 GRQSSNPSSMESCFTWELLAIAIGELVSQGLIDEDKLDTFNVPSYFPSLEEVKDIVERDG 281

Query: 420 SFAIESTKTIAHHVENEID-------DKWERSKSLARLIRAFTEALIPHHFGEEVVGPVY 578
           SF      TI H V  E+D       DKW R + LA+ +RAFTE +I + FG E++  +Y
Sbjct: 282 SF------TIDHMVGFELDTPQMQENDKWVRVEKLAKAVRAFTEPIISNQFGHEIMDKLY 335

Query: 579 DKLAHCAFEYLD 614
           DK  +     L+
Sbjct: 336 DKFTYIVVSDLE 347


>gb|EOY17880.1| S-adenosyl-L-methionine-dependent methyltransferases superfamily
           protein isoform 1 [Theobroma cacao]
           gi|508725988|gb|EOY17885.1|
           S-adenosyl-L-methionine-dependent methyltransferases
           superfamily protein isoform 2 [Theobroma cacao]
          Length = 364

 Score = 86.3 bits (212), Expect(2) = 4e-34
 Identities = 39/81 (48%), Positives = 58/81 (71%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           S+H V++ Y++HW S+APK + +E G P+NKG +Y+S+TS   V KAY++QF+EDF+  L
Sbjct: 144 SMHLVYSCYSVHWLSEAPK-ITNEAGLPLNKGKIYMSKTSPPAVTKAYLSQFQEDFSSLL 202

Query: 187 ACRSQEVVPKGRMLLTVEDAQ 249
             RSQE+ P GR++L     Q
Sbjct: 203 KFRSQELAPNGRVVLIFNGRQ 223



 Score = 85.9 bits (211), Expect(2) = 4e-34
 Identities = 51/137 (37%), Positives = 76/137 (55%), Gaps = 1/137 (0%)
 Frame = +3

Query: 255 SNSLTWQPWELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKKQGSFAIE 434
           +N  T   W+L    +ALS L+ +GL+ E KLD+FN PYY  S+E++K +V K+GS  IE
Sbjct: 228 TNKDTCYTWDL--LAEALSYLVSQGLVDEGKLDSFNVPYYNPSQEEIKYLVDKEGSLTIE 285

Query: 435 STKTIAHHVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDKLAHCAFEYLD 614
              TI   +    +  W   +S  R  R FTE L+ H FGE ++  +YDK      E   
Sbjct: 286 FIDTIELEIGGP-NGYWSSPESRIRGHRCFTEPLLSHQFGERLMDKLYDKATQILVEDYK 344

Query: 615 HGLFAMHHT-VSILLRK 662
           HG  A  +  ++++L+K
Sbjct: 345 HGKEATKNIGIAVVLKK 361


>gb|EOY17881.1| S-adenosyl-L-methionine-dependent methyltransferases superfamily
           protein isoform 2, partial [Theobroma cacao]
          Length = 362

 Score = 86.3 bits (212), Expect(2) = 4e-34
 Identities = 39/81 (48%), Positives = 58/81 (71%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           S+H V++ Y++HW S+APK + +E G P+NKG +Y+S+TS   V KAY++QF+EDF+  L
Sbjct: 144 SMHLVYSCYSVHWLSEAPK-ITNEAGLPLNKGKIYMSKTSPPAVTKAYLSQFQEDFSSLL 202

Query: 187 ACRSQEVVPKGRMLLTVEDAQ 249
             RSQE+ P GR++L     Q
Sbjct: 203 KFRSQELAPNGRVVLIFNGRQ 223



 Score = 85.9 bits (211), Expect(2) = 4e-34
 Identities = 51/137 (37%), Positives = 76/137 (55%), Gaps = 1/137 (0%)
 Frame = +3

Query: 255 SNSLTWQPWELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKKQGSFAIE 434
           +N  T   W+L    +ALS L+ +GL+ E KLD+FN PYY  S+E++K +V K+GS  IE
Sbjct: 228 TNKDTCYTWDL--LAEALSYLVSQGLVDEGKLDSFNVPYYNPSQEEIKYLVDKEGSLTIE 285

Query: 435 STKTIAHHVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDKLAHCAFEYLD 614
              TI   +    +  W   +S  R  R FTE L+ H FGE ++  +YDK      E   
Sbjct: 286 FIDTIELEIGGP-NGYWSSPESRIRGHRCFTEPLLSHQFGERLMDKLYDKATQILVEDYK 344

Query: 615 HGLFAMHHT-VSILLRK 662
           HG  A  +  ++++L+K
Sbjct: 345 HGKEATKNIGIAVVLKK 361


>gb|EOY17887.1| S-adenosyl-L-methionine-dependent methyltransferases superfamily
           protein isoform 4 [Theobroma cacao]
          Length = 335

 Score = 86.3 bits (212), Expect(2) = 4e-34
 Identities = 39/81 (48%), Positives = 58/81 (71%)
 Frame = +1

Query: 7   SLHFVHASYTLHWFSQAPKALCSEQGGPINKGNVYISETSCELVAKAYMAQFEEDFTQFL 186
           S+H V++ Y++HW S+APK + +E G P+NKG +Y+S+TS   V KAY++QF+EDF+  L
Sbjct: 115 SMHLVYSCYSVHWLSEAPK-ITNEAGLPLNKGKIYMSKTSPPAVTKAYLSQFQEDFSSLL 173

Query: 187 ACRSQEVVPKGRMLLTVEDAQ 249
             RSQE+ P GR++L     Q
Sbjct: 174 KFRSQELAPNGRVVLIFNGRQ 194



 Score = 85.9 bits (211), Expect(2) = 4e-34
 Identities = 51/137 (37%), Positives = 76/137 (55%), Gaps = 1/137 (0%)
 Frame = +3

Query: 255 SNSLTWQPWELKIFTKALSCLLFEGLIKEEKLDTFNFPYYCASEEDLKKIVKKQGSFAIE 434
           +N  T   W+L    +ALS L+ +GL+ E KLD+FN PYY  S+E++K +V K+GS  IE
Sbjct: 199 TNKDTCYTWDL--LAEALSYLVSQGLVDEGKLDSFNVPYYNPSQEEIKYLVDKEGSLTIE 256

Query: 435 STKTIAHHVENEIDDKWERSKSLARLIRAFTEALIPHHFGEEVVGPVYDKLAHCAFEYLD 614
              TI   +    +  W   +S  R  R FTE L+ H FGE ++  +YDK      E   
Sbjct: 257 FIDTIELEIGGP-NGYWSSPESRIRGHRCFTEPLLSHQFGERLMDKLYDKATQILVEDYK 315

Query: 615 HGLFAMHHT-VSILLRK 662
           HG  A  +  ++++L+K
Sbjct: 316 HGKEATKNIGIAVVLKK 332