BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Achyranthes22_contig00064895
         (269 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004512245.1| PREDICTED: probable acyl-activating enzyme 1...    92   7e-17
gb|EOX90703.1| Acyl activating enzyme 1 [Theobroma cacao]              90   4e-16
ref|XP_003612316.1| 2-succinylbenzoate-CoA ligase [Medicago trun...    87   2e-15
ref|XP_006340954.1| PREDICTED: probable acyl-activating enzyme 2...    84   1e-14
ref|YP_002462908.1| acyl-CoA synthetase [Chloroflexus aggregans ...    84   3e-14
ref|XP_004289047.1| PREDICTED: probable acyl-activating enzyme 1...    83   3e-14
ref|XP_002337088.1| predicted protein [Populus trichocarpa] gi|5...    83   4e-14
ref|XP_006467053.1| PREDICTED: probable acyl-activating enzyme 1...    82   1e-13
ref|XP_006425312.1| hypothetical protein CICLE_v10027208mg [Citr...    81   2e-13
gb|EMJ00977.1| hypothetical protein PRUPE_ppa003716mg [Prunus pe...    81   2e-13
ref|YP_001635492.1| acyl-CoA synthetase [Chloroflexus aurantiacu...    80   2e-13
gb|ESW29972.1| hypothetical protein PHAVU_002G114400g [Phaseolus...    80   2e-13
ref|XP_002521692.1| AMP dependent ligase, putative [Ricinus comm...    80   2e-13
ref|XP_004290510.1| PREDICTED: probable acyl-activating enzyme 1...    80   3e-13
ref|WP_009763961.1| acyl-CoA synthetase [Microvirga lotononidis]...    80   3e-13
ref|XP_002307223.2| hypothetical protein POPTR_0005s10590g, part...    79   5e-13
ref|XP_004511496.1| PREDICTED: probable acyl-activating enzyme 1...    79   5e-13
ref|XP_004232549.1| PREDICTED: probable acyl-activating enzyme 1...    79   5e-13
ref|XP_003538902.1| PREDICTED: probable acyl-activating enzyme 1...    79   6e-13
ref|YP_003070595.1| AMP-dependent synthetase/acyl-CoA ligase [Me...    79   6e-13

>ref|XP_004512245.1| PREDICTED: probable acyl-activating enzyme 1, peroxisomal-like
           [Cicer arietinum]
          Length = 547

 Score = 92.0 bits (227), Expect = 7e-17
 Identities = 52/93 (55%), Positives = 62/93 (66%), Gaps = 4/93 (4%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVRKL-QNCSY-AKKLNN--NPSFMDHIMEEFDVKDPMTMQSV 100
           GF+V   YGMTE+LGP +VRK  QN      KLN       +D +M E DVKDP TM+SV
Sbjct: 323 GFDVNIGYGMTEVLGPVIVRKWKQNLDDDITKLNYYVEKGLIDFMMVEVDVKDPNTMKSV 382

Query: 99  PWDGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
           P+DG TIGE+MFKGN LM+GYLK+      AFK
Sbjct: 383 PYDGRTIGEIMFKGNTLMLGYLKSSQMIEEAFK 415


>gb|EOX90703.1| Acyl activating enzyme 1 [Theobroma cacao]
          Length = 553

 Score = 89.7 bits (221), Expect = 4e-16
 Identities = 49/95 (51%), Positives = 63/95 (66%), Gaps = 6/95 (6%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVRKLQ----NC--SYAKKLNNNPSFMDHIMEEFDVKDPMTMQ 106
           GF V Y YGMTE+LGP +VR  +    +C  +  +K   + +    ++EE DVKDP TM+
Sbjct: 322 GFSVSYAYGMTEVLGPAIVRPWRAEPDSCLSNEHRKDKFHEALRRLLIEEIDVKDPDTMK 381

Query: 105 SVPWDGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
           SVP +G TIGEVMF+GN LM GYLK P  T+ AFK
Sbjct: 382 SVPRNGKTIGEVMFRGNTLMSGYLKNPKATQEAFK 416


>ref|XP_003612316.1| 2-succinylbenzoate-CoA ligase [Medicago truncatula]
           gi|355513651|gb|AES95274.1| 2-succinylbenzoate-CoA
           ligase [Medicago truncatula]
          Length = 550

 Score = 87.0 bits (214), Expect = 2e-15
 Identities = 48/93 (51%), Positives = 60/93 (64%), Gaps = 4/93 (4%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVRKLQNC--SYAKKLN--NNPSFMDHIMEEFDVKDPMTMQSV 100
           GF+V   YGMTE LGP +VRK +        KLN  +    +D +M E DVKDP TM+SV
Sbjct: 323 GFDVNIGYGMTETLGPVIVRKWKQNFDDDITKLNYVDKKGVIDFMMVEVDVKDPNTMKSV 382

Query: 99  PWDGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
           P+DG TIGE+MFKGN  M GYLK+   +  AF+
Sbjct: 383 PYDGKTIGEIMFKGNTSMSGYLKSSQVSHEAFR 415


>ref|XP_006340954.1| PREDICTED: probable acyl-activating enzyme 2-like [Solanum
           tuberosum]
          Length = 561

 Score = 84.3 bits (207), Expect = 1e-14
 Identities = 47/97 (48%), Positives = 62/97 (63%), Gaps = 8/97 (8%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVR--KLQNCSYAKKLNNNPSFM------DHIMEEFDVKDPMT 112
           GF + + YGM+E LGP +    K Q+  Y  KLN +          + +MEE DVKDP +
Sbjct: 324 GFTINHAYGMSEALGPMISMPWKCQD-EYLSKLNEDVDVKIREGVHNIMMEEVDVKDPKS 382

Query: 111 MQSVPWDGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
           M+SVP DG TIGE+MF+GNA+MMGYLK  + T  AF+
Sbjct: 383 MESVPADGKTIGEIMFRGNAMMMGYLKDSSRTEEAFE 419


>ref|YP_002462908.1| acyl-CoA synthetase [Chloroflexus aggregans DSM 9485]
           gi|506420612|ref|WP_015940331.1| acyl-CoA synthetase
           [Chloroflexus aggregans] gi|219542734|gb|ACL24472.1|
           AMP-dependent synthetase and ligase [Chloroflexus
           aggregans DSM 9485]
          Length = 549

 Score = 83.6 bits (205), Expect = 3e-14
 Identities = 44/95 (46%), Positives = 53/95 (55%), Gaps = 6/95 (6%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVRKLQN------CSYAKKLNNNPSFMDHIMEEFDVKDPMTMQ 106
           GFE+ + YG+TE  GP  V   Q            +LN       H  E   V DP+TMQ
Sbjct: 323 GFEITHVYGLTETYGPAAVCAKQPDWHRLPIEEQARLNGRQGVNYHAEEAVSVLDPVTMQ 382

Query: 105 SVPWDGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
            VPWDG T+GEVMF+GN +M GYLK PT T  AF+
Sbjct: 383 PVPWDGQTMGEVMFRGNIVMKGYLKNPTATEAAFR 417


>ref|XP_004289047.1| PREDICTED: probable acyl-activating enzyme 1, peroxisomal-like
           [Fragaria vesca subsp. vesca]
          Length = 544

 Score = 83.2 bits (204), Expect = 3e-14
 Identities = 44/89 (49%), Positives = 56/89 (62%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVRKLQNCSYAKKLNNNPSFMDHIMEEFDVKDPMTMQSVPWDG 88
           GF V + YGMTE LGP +V   +      +L +  + M   MEE DVKDP TM+SVP+DG
Sbjct: 332 GFNVSHGYGMTEALGPAIVTPCK-----PELESQYNLM---MEEVDVKDPSTMESVPYDG 383

Query: 87  TTIGEVMFKGNALMMGYLKAPTETRNAFK 1
            T+GE+MF+GN LM+GYLK        FK
Sbjct: 384 KTMGEIMFRGNTLMLGYLKNSKAVNEPFK 412


>ref|XP_002337088.1| predicted protein [Populus trichocarpa]
           gi|566180361|ref|XP_006380574.1| hypothetical protein
           POPTR_0007s09420g [Populus trichocarpa]
           gi|550334462|gb|ERP58371.1| hypothetical protein
           POPTR_0007s09420g [Populus trichocarpa]
          Length = 511

 Score = 82.8 bits (203), Expect = 4e-14
 Identities = 46/95 (48%), Positives = 60/95 (63%), Gaps = 6/95 (6%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVR----KLQNC--SYAKKLNNNPSFMDHIMEEFDVKDPMTMQ 106
           GF V + YGMTE LGP +VR    +L +   +  +K+ N     + ++E  DVKDP TM+
Sbjct: 285 GFNVYHGYGMTEALGPAIVRPWKPELDSTFENEQEKIRNREGLHNLLIEGVDVKDPNTMK 344

Query: 105 SVPWDGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
           SVP DG TIGEVMF+ N LM GYLK    T+ AF+
Sbjct: 345 SVPRDGKTIGEVMFRSNILMSGYLKNSEVTQEAFR 379


>ref|XP_006467053.1| PREDICTED: probable acyl-activating enzyme 1, peroxisomal-like
           [Citrus sinensis]
          Length = 562

 Score = 81.6 bits (200), Expect = 1e-13
 Identities = 40/96 (41%), Positives = 59/96 (61%), Gaps = 7/96 (7%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVRKLQNCSYAKKLNNNPSFMDH-------IMEEFDVKDPMTM 109
           GF + + YGMTE+LGP +VR+ +    + +L++      H       +++  DVKD  TM
Sbjct: 335 GFNIRHAYGMTEVLGPAIVRQWKPEGESSELDHEHEMTKHREGLNNLLIQGVDVKDQNTM 394

Query: 108 QSVPWDGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
           +SVP DG T+GEVMF+ N +M+GY K P  T  AF+
Sbjct: 395 KSVPHDGKTLGEVMFRANTVMLGYFKNPKATEEAFR 430


>ref|XP_006425312.1| hypothetical protein CICLE_v10027208mg [Citrus clementina]
           gi|557527302|gb|ESR38552.1| hypothetical protein
           CICLE_v10027208mg [Citrus clementina]
          Length = 562

 Score = 80.9 bits (198), Expect = 2e-13
 Identities = 40/96 (41%), Positives = 58/96 (60%), Gaps = 7/96 (7%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVRKLQNCSYAKKLNNNPSFMDH-------IMEEFDVKDPMTM 109
           GF + + YGMTE+LGP +VR+ +    +  L++      H       +++  DVKD  TM
Sbjct: 335 GFNIRHAYGMTEVLGPAIVRQWKPEGESSALDHEHEMTKHREGLNNLLIQGVDVKDQNTM 394

Query: 108 QSVPWDGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
           +SVP DG T+GEVMF+ N +M+GY K P  T  AF+
Sbjct: 395 KSVPHDGKTLGEVMFRANTVMLGYFKNPKATEEAFR 430


>gb|EMJ00977.1| hypothetical protein PRUPE_ppa003716mg [Prunus persica]
          Length = 554

 Score = 80.9 bits (198), Expect = 2e-13
 Identities = 46/100 (46%), Positives = 60/100 (60%), Gaps = 11/100 (11%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVRKLQNCSYAKKLNNNPSF----------MDHI-MEEFDVKD 121
           GF V + YG+TE  GP  V     CS+  + ++ P            + H+ MEE DVKD
Sbjct: 323 GFNVTHSYGLTETYGPGTV-----CSWKPEWDSLPRVEQAKIKSRQGLQHLGMEELDVKD 377

Query: 120 PMTMQSVPWDGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
           P+TM+SVP D  T+GEVMF+GN +M GYLK    TR+AFK
Sbjct: 378 PVTMKSVPSDAKTMGEVMFRGNTVMNGYLKDHQATRDAFK 417


>ref|YP_001635492.1| acyl-CoA synthetase [Chloroflexus aurantiacus J-10-fl]
           gi|222525298|ref|YP_002569769.1| acyl-CoA synthetase
           [Chloroflexus sp. Y-400-fl]
           gi|501214739|ref|WP_012257757.1| acyl-CoA synthetase
           [Chloroflexus] gi|163668737|gb|ABY35103.1| AMP-dependent
           synthetase and ligase [Chloroflexus aurantiacus J-10-fl]
           gi|222449177|gb|ACM53443.1| AMP-dependent synthetase and
           ligase [Chloroflexus sp. Y-400-fl]
          Length = 548

 Score = 80.5 bits (197), Expect = 2e-13
 Identities = 42/95 (44%), Positives = 51/95 (53%), Gaps = 6/95 (6%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVRKLQN------CSYAKKLNNNPSFMDHIMEEFDVKDPMTMQ 106
           GF++ + YG+TE  GP  V   Q        +    LN       H  E   V DP TMQ
Sbjct: 322 GFDITHVYGLTETYGPAAVCAKQPDWHTLPIAEQAHLNGRQGVTYHAQEAISVLDPATMQ 381

Query: 105 SVPWDGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
            VPWDG T+GEVMF+GN +M GYLK P  T  AF+
Sbjct: 382 PVPWDGQTMGEVMFRGNIVMKGYLKNPAATEAAFR 416


>gb|ESW29972.1| hypothetical protein PHAVU_002G114400g [Phaseolus vulgaris]
          Length = 459

 Score = 80.5 bits (197), Expect = 2e-13
 Identities = 43/89 (48%), Positives = 55/89 (61%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVRKLQNCSYAKKLNNNPSFMDHIMEEFDVKDPMTMQSVPWDG 88
           GF+V   YGMTE  GP +VR     S ++    N   M   M+  DVKDP T +S P DG
Sbjct: 241 GFDVNIGYGMTETYGPFIVRPWNPTSDSEHTKLNYG-MSGFMQNVDVKDPKTNESTPCDG 299

Query: 87  TTIGEVMFKGNALMMGYLKAPTETRNAFK 1
            TIGE++FKG+ALM+GYLK P  T+ A +
Sbjct: 300 KTIGEIVFKGDALMVGYLKNPEATKEAIR 328


>ref|XP_002521692.1| AMP dependent ligase, putative [Ricinus communis]
           gi|223539083|gb|EEF40679.1| AMP dependent ligase,
           putative [Ricinus communis]
          Length = 428

 Score = 80.5 bits (197), Expect = 2e-13
 Identities = 43/90 (47%), Positives = 58/90 (64%), Gaps = 2/90 (2%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVR--KLQNCSYAKKLNNNPSFMDHIMEEFDVKDPMTMQSVPW 94
           GF V   YGMTE+LGP +VR  KL++    +K+ ++    + ++E  DVKDP TM+SVP 
Sbjct: 220 GFNVADGYGMTEVLGPAIVRPWKLES-DEQEKIKSSEGLHNILIEGVDVKDPNTMKSVPH 278

Query: 93  DGTTIGEVMFKGNALMMGYLKAPTETRNAF 4
           DG T+GEVMF+ N LM GY      T+ AF
Sbjct: 279 DGKTVGEVMFRSNILMSGYFNNVEATQEAF 308


>ref|XP_004290510.1| PREDICTED: probable acyl-activating enzyme 1, peroxisomal-like
           [Fragaria vesca subsp. vesca]
          Length = 554

 Score = 80.1 bits (196), Expect = 3e-13
 Identities = 45/100 (45%), Positives = 59/100 (59%), Gaps = 11/100 (11%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVRKLQNCSYAKKLNNNP----------SFMDHI-MEEFDVKD 121
           GF V + YG+TE  GP  V     CS+  + N+ P            + HI MEE DVKD
Sbjct: 323 GFSVTHSYGLTETYGPGTV-----CSWKPEWNSLPRDEQAKIKSRQGLQHIGMEELDVKD 377

Query: 120 PMTMQSVPWDGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
           P+TM+S P+D  T+GEVMF+GN +M GY K    T++AFK
Sbjct: 378 PVTMKSAPFDAKTMGEVMFRGNPVMNGYFKDNKSTQDAFK 417


>ref|WP_009763961.1| acyl-CoA synthetase [Microvirga lotononidis]
           gi|388587618|gb|EIM27911.1| acyl-CoA synthetase
           (AMP-forming)/AMP-acid ligase II [Microvirga
           lotononidis]
          Length = 546

 Score = 80.1 bits (196), Expect = 3e-13
 Identities = 44/96 (45%), Positives = 56/96 (58%), Gaps = 7/96 (7%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVRK-------LQNCSYAKKLNNNPSFMDHIMEEFDVKDPMTM 109
           GF+V + YG+TE+ GP VV +       L    YA K          ++E  DV DP TM
Sbjct: 322 GFDVTHLYGLTEVYGPAVVNEWHDEWDALDQGGYAAK-KARQGVRYPVLEALDVLDPETM 380

Query: 108 QSVPWDGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
           Q VP DG T+GEVMF+GN +M GYLK P+ T  AF+
Sbjct: 381 QPVPADGRTLGEVMFRGNVVMKGYLKNPSGTAKAFE 416


>ref|XP_002307223.2| hypothetical protein POPTR_0005s10590g, partial [Populus
           trichocarpa] gi|550338579|gb|EEE94219.2| hypothetical
           protein POPTR_0005s10590g, partial [Populus trichocarpa]
          Length = 537

 Score = 79.3 bits (194), Expect = 5e-13
 Identities = 43/95 (45%), Positives = 57/95 (60%), Gaps = 6/95 (6%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVRKLQNCSYA------KKLNNNPSFMDHIMEEFDVKDPMTMQ 106
           GF V + YGMTE LGP  +R  +  S +      +++       + ++E  DVKDP TM+
Sbjct: 311 GFNVYHGYGMTEALGPATIRPWKPESDSTFEIEQEEIRRREGLHNLLLEGVDVKDPKTME 370

Query: 105 SVPWDGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
           SVP DG TIGEVMF+GN LM GYLK+   T+   K
Sbjct: 371 SVPNDGKTIGEVMFRGNILMSGYLKSTEITQETLK 405


>ref|XP_004511496.1| PREDICTED: probable acyl-activating enzyme 1, peroxisomal-like
           isoform X1 [Cicer arietinum]
          Length = 552

 Score = 79.3 bits (194), Expect = 5e-13
 Identities = 44/100 (44%), Positives = 60/100 (60%), Gaps = 11/100 (11%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVRKLQNCSYAKKLNNNP----------SFMDHI-MEEFDVKD 121
           GF+V + YG+TE  GP  +     C++  +  + P            + HI MEE DVKD
Sbjct: 320 GFDVTHSYGLTETYGPASI-----CTWKSEWGSLPRDAQAKLKARQGVQHIGMEELDVKD 374

Query: 120 PMTMQSVPWDGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
           P+TM+SVP D  T+GEVMF+GN +M GYLK    T++AFK
Sbjct: 375 PVTMKSVPADAKTLGEVMFRGNTVMSGYLKDSKATQDAFK 414


>ref|XP_004232549.1| PREDICTED: probable acyl-activating enzyme 1, peroxisomal-like
           [Solanum lycopersicum]
          Length = 565

 Score = 79.3 bits (194), Expect = 5e-13
 Identities = 43/96 (44%), Positives = 60/96 (62%), Gaps = 7/96 (7%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVR--KLQNCSYAKKLNN-----NPSFMDHIMEEFDVKDPMTM 109
           GF + + YGM+E LGP +    K Q+  ++K   +          + +MEE DVKDP +M
Sbjct: 324 GFTINHAYGMSEALGPMISMPWKFQDEHFSKSNQDVDVKIREGVHNIMMEEVDVKDPESM 383

Query: 108 QSVPWDGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
           +SVP DG T GE+MF+GNA+MMGYLK  + T  AF+
Sbjct: 384 KSVPADGKTTGEIMFRGNAMMMGYLKDSSRTEEAFE 419


>ref|XP_003538902.1| PREDICTED: probable acyl-activating enzyme 1, peroxisomal-like
           [Glycine max]
          Length = 541

 Score = 79.0 bits (193), Expect = 6e-13
 Identities = 46/91 (50%), Positives = 55/91 (60%), Gaps = 2/91 (2%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVRKLQNCSYAK--KLNNNPSFMDHIMEEFDVKDPMTMQSVPW 94
           GF+V   YGMTE LGP +VR     S  +  KLN   S      ++ DVKDP T +S P 
Sbjct: 321 GFDVNIGYGMTETLGPVIVRPWNPNSDGEHTKLNYGVS---EFRQDVDVKDPETGESTPH 377

Query: 93  DGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
           DG TIGE+MFKGNALM+GYLK       AF+
Sbjct: 378 DGKTIGEIMFKGNALMLGYLKNSQANDKAFR 408


>ref|YP_003070595.1| AMP-dependent synthetase/acyl-CoA ligase [Methylobacterium
           extorquens DM4] gi|506304506|ref|WP_015824281.1|
           acyl-CoA synthetase [Methylobacterium extorquens]
           gi|254270778|emb|CAX26783.1| putative AMP-dependent
           synthetase/acyl-CoA ligase [Methylobacterium extorquens
           DM4]
          Length = 545

 Score = 79.0 bits (193), Expect = 6e-13
 Identities = 43/96 (44%), Positives = 56/96 (58%), Gaps = 7/96 (7%)
 Frame = -3

Query: 267 GFEVVYRYGMTEILGPPVVRK-------LQNCSYAKKLNNNPSFMDHIMEEFDVKDPMTM 109
           GF+V + YG+TE  GP VV         L     A+K          ++E  DV+DP TM
Sbjct: 323 GFDVTHLYGLTETYGPAVVNAWHEDWDALSRDEQARK-KARQGVRYPVLEGLDVRDPETM 381

Query: 108 QSVPWDGTTIGEVMFKGNALMMGYLKAPTETRNAFK 1
           +S+P DGT++GEVMF+GN +M GYLK P  T  AFK
Sbjct: 382 ESLPADGTSLGEVMFRGNVVMRGYLKNPASTEAAFK 417