BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Achyranthes22_contig00057573
         (462 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004301409.1| PREDICTED: isoamylase 1, chloroplastic-like ...   100   3e-19
ref|XP_006474309.1| PREDICTED: isoamylase 1, chloroplastic-like ...    86   7e-15
ref|XP_006453203.1| hypothetical protein CICLE_v100074892mg, par...    86   7e-15
gb|EOY32085.1| Isoamylase 1 isoform 2 [Theobroma cacao]                81   1e-13
gb|EOY32084.1| Isoamylase 1 isoform 1 [Theobroma cacao]                81   1e-13
ref|XP_002308090.2| isoamylase family protein [Populus trichocar...    80   2e-13
ref|XP_006381137.1| hypothetical protein POPTR_0006s07010g [Popu...    80   2e-13
ref|XP_002324659.2| isoamylase family protein [Populus trichocar...    79   8e-13
ref|XP_006295820.1| hypothetical protein CARUB_v10024947mg [Caps...    76   5e-12
ref|XP_006411237.1| hypothetical protein EUTSA_v10016275mg [Eutr...    75   7e-12
ref|XP_002879833.1| ATISA1/ISA1 [Arabidopsis lyrata subsp. lyrat...    75   9e-12
gb|EMJ26281.1| hypothetical protein PRUPE_ppa025097mg, partial [...    75   1e-11
ref|NP_181522.1| isoamylase 1 [Arabidopsis thaliana] gi|75096955...    74   3e-11
ref|XP_002529900.1| isoamylase, putative [Ricinus communis] gi|2...    73   3e-11
ref|XP_004242868.1| PREDICTED: isoamylase 1, chloroplastic-like ...    72   6e-11
ref|XP_004163012.1| PREDICTED: isoamylase 1, chloroplastic-like ...    72   1e-10
ref|XP_004149676.1| PREDICTED: isoamylase 1, chloroplastic-like ...    72   1e-10
ref|XP_003532455.2| PREDICTED: isoamylase 1, chloroplastic-like ...    71   1e-10
gb|EXC12839.1| Isoamylase 1 [Morus notabilis]                          71   2e-10
gb|AAY89378.1| isoamylase isoform 1 [Nicotiana langsdorffii x Ni...    71   2e-10

>ref|XP_004301409.1| PREDICTED: isoamylase 1, chloroplastic-like [Fragaria vesca subsp.
           vesca]
          Length = 794

 Score = 99.8 bits (247), Expect = 3e-19
 Identities = 50/116 (43%), Positives = 74/116 (63%)
 Frame = +2

Query: 113 SSPPKFFNPHKQFSLNLTTRISSPFTSIISTGHLQISRTNPPLMVNARDTTTSSAEVVDT 292
           +SPPK ++PH         R+  P  + ++   L   R  PP+  N      ++AE V+T
Sbjct: 8   ASPPKLYSPHHHNRFASNARLPGP--NRLNRPMLGFRRHAPPVKANDGGGAGTTAEAVET 65

Query: 293 FVTEKSSETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSVSATLCLITISDLQQN 460
            V E+S +  RF+V +G P PFGAT CDGGVNFA+++G++VSAT+CLIT +DL+Q+
Sbjct: 66  AVVERSPKLRRFQVSDGRPNPFGATPCDGGVNFAVYAGNAVSATVCLITPADLEQD 121


>ref|XP_006474309.1| PREDICTED: isoamylase 1, chloroplastic-like [Citrus sinensis]
          Length = 805

 Score = 85.5 bits (210), Expect = 7e-15
 Identities = 56/134 (41%), Positives = 75/134 (55%), Gaps = 7/134 (5%)
 Frame = +2

Query: 80  MELASRTPFPLSSPPKFFNPHKQFSLNLTTRISSPFTSII-------STGHLQISRTNPP 238
           MEL   T  PL      F    +F  +  + +  P T+          T H   S+    
Sbjct: 1   MELLQFTSSPLLHGSSNFINSPEFYKSKLSNLKKPPTTTFRCCNHPNKTSHFANSKAFEN 60

Query: 239 LMVNARDTTTSSAEVVDTFVTEKSSETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSVS 418
           +  N     + SAE+ +T V EK   + RF+V +GYPTPFGATL DGGVNF+IFS ++VS
Sbjct: 61  VTKNLVIRASKSAEL-ETAVIEKPP-SQRFQVSKGYPTPFGATLRDGGVNFSIFSSNAVS 118

Query: 419 ATLCLITISDLQQN 460
           A+LCLIT+SDLQ+N
Sbjct: 119 ASLCLITLSDLQEN 132


>ref|XP_006453203.1| hypothetical protein CICLE_v100074892mg, partial [Citrus
           clementina] gi|557556429|gb|ESR66443.1| hypothetical
           protein CICLE_v100074892mg, partial [Citrus clementina]
          Length = 624

 Score = 85.5 bits (210), Expect = 7e-15
 Identities = 56/134 (41%), Positives = 75/134 (55%), Gaps = 7/134 (5%)
 Frame = +2

Query: 80  MELASRTPFPLSSPPKFFNPHKQFSLNLTTRISSPFTSII-------STGHLQISRTNPP 238
           MEL   T  PL      F    +F  +  + +  P T+          T H   S+    
Sbjct: 1   MELLQFTSSPLLHGSSNFINSPEFYKSKLSNLKKPPTTTFRCCNHPNKTSHFANSKAFEN 60

Query: 239 LMVNARDTTTSSAEVVDTFVTEKSSETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSVS 418
           +  N     + SAE+ +T V EK   + RF+V +GYPTPFGATL DGGVNF+IFS ++VS
Sbjct: 61  VTKNLVIRASKSAEL-ETAVIEKPP-SQRFQVSKGYPTPFGATLRDGGVNFSIFSSNAVS 118

Query: 419 ATLCLITISDLQQN 460
           A+LCLIT+SDLQ+N
Sbjct: 119 ASLCLITLSDLQEN 132


>gb|EOY32085.1| Isoamylase 1 isoform 2 [Theobroma cacao]
          Length = 795

 Score = 81.3 bits (199), Expect = 1e-13
 Identities = 49/97 (50%), Positives = 59/97 (60%), Gaps = 4/97 (4%)
 Frame = +2

Query: 182 PFTSIISTGHLQISRTNPPLMVNARDT----TTSSAEVVDTFVTEKSSETMRFEVFEGYP 349
           P    +S  H   S T   ++V AR          A   DT V EK  +  RF++ EG+P
Sbjct: 29  PNLRTVSNNHATTSTTR--MVVKARGGGGGGARRGAAEADTAVVEKP-KIKRFQLSEGHP 85

Query: 350 TPFGATLCDGGVNFAIFSGHSVSATLCLITISDLQQN 460
            PFGATL DGGVNFAI+S ++VSATLCLIT SDLQQN
Sbjct: 86  GPFGATLLDGGVNFAIYSANAVSATLCLITFSDLQQN 122


>gb|EOY32084.1| Isoamylase 1 isoform 1 [Theobroma cacao]
          Length = 904

 Score = 81.3 bits (199), Expect = 1e-13
 Identities = 49/97 (50%), Positives = 59/97 (60%), Gaps = 4/97 (4%)
 Frame = +2

Query: 182 PFTSIISTGHLQISRTNPPLMVNARDT----TTSSAEVVDTFVTEKSSETMRFEVFEGYP 349
           P    +S  H   S T   ++V AR          A   DT V EK  +  RF++ EG+P
Sbjct: 29  PNLRTVSNNHATTSTTR--MVVKARGGGGGGARRGAAEADTAVVEKP-KIKRFQLSEGHP 85

Query: 350 TPFGATLCDGGVNFAIFSGHSVSATLCLITISDLQQN 460
            PFGATL DGGVNFAI+S ++VSATLCLIT SDLQQN
Sbjct: 86  GPFGATLLDGGVNFAIYSANAVSATLCLITFSDLQQN 122


>ref|XP_002308090.2| isoamylase family protein [Populus trichocarpa]
           gi|550335674|gb|EEE91613.2| isoamylase family protein
           [Populus trichocarpa]
          Length = 876

 Score = 80.5 bits (197), Expect = 2e-13
 Identities = 50/116 (43%), Positives = 70/116 (60%), Gaps = 3/116 (2%)
 Frame = +2

Query: 122 PKFFNPHKQFSLNLTTRISSPFTSIISTGHLQISRTNPPLMVNARDTTTSSAEVVDTFVT 301
           PKF+ P +  + NL T  S+ F++  ST  + I                +++E VDT V 
Sbjct: 74  PKFYPPKRVTTSNLETIGSNIFSN--STALIPIK---------------AASEGVDTAVV 116

Query: 302 ---EKSSETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSVSATLCLITISDLQQN 460
              E+  +  + +VFEG+P PFGAT+ DGGVNFAIFS  +VSATLCLI++SDL +N
Sbjct: 117 VVEEEEPKLKKIQVFEGHPAPFGATVRDGGVNFAIFSADAVSATLCLISLSDLPEN 172


>ref|XP_006381137.1| hypothetical protein POPTR_0006s07010g [Populus trichocarpa]
           gi|550335673|gb|ERP58934.1| hypothetical protein
           POPTR_0006s07010g [Populus trichocarpa]
          Length = 845

 Score = 80.5 bits (197), Expect = 2e-13
 Identities = 50/116 (43%), Positives = 70/116 (60%), Gaps = 3/116 (2%)
 Frame = +2

Query: 122 PKFFNPHKQFSLNLTTRISSPFTSIISTGHLQISRTNPPLMVNARDTTTSSAEVVDTFVT 301
           PKF+ P +  + NL T  S+ F++  ST  + I                +++E VDT V 
Sbjct: 74  PKFYPPKRVTTSNLETIGSNIFSN--STALIPIK---------------AASEGVDTAVV 116

Query: 302 ---EKSSETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSVSATLCLITISDLQQN 460
              E+  +  + +VFEG+P PFGAT+ DGGVNFAIFS  +VSATLCLI++SDL +N
Sbjct: 117 VVEEEEPKLKKIQVFEGHPAPFGATVRDGGVNFAIFSADAVSATLCLISLSDLPEN 172


>ref|XP_002324659.2| isoamylase family protein [Populus trichocarpa]
           gi|550318648|gb|EEF03224.2| isoamylase family protein
           [Populus trichocarpa]
          Length = 794

 Score = 78.6 bits (192), Expect = 8e-13
 Identities = 49/115 (42%), Positives = 69/115 (60%)
 Frame = +2

Query: 116 SPPKFFNPHKQFSLNLTTRISSPFTSIISTGHLQISRTNPPLMVNARDTTTSSAEVVDTF 295
           S PKF    +  + NL    S+ F++  ST  + I          ARD   ++       
Sbjct: 22  STPKFCLLKRNVTSNLENFGSNLFSN--STASISIKA--------ARDGVDTAV-----V 66

Query: 296 VTEKSSETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSVSATLCLITISDLQQN 460
           V E+  +  +F+VFEG+P+PFGAT+ DGGVNFAIFS ++VSATLCLI++SDL +N
Sbjct: 67  VEEEGPKLRKFQVFEGHPSPFGATVRDGGVNFAIFSANAVSATLCLISLSDLPEN 121


>ref|XP_006295820.1| hypothetical protein CARUB_v10024947mg [Capsella rubella]
           gi|482564528|gb|EOA28718.1| hypothetical protein
           CARUB_v10024947mg [Capsella rubella]
          Length = 783

 Score = 75.9 bits (185), Expect = 5e-12
 Identities = 38/75 (50%), Positives = 56/75 (74%)
 Frame = +2

Query: 236 PLMVNARDTTTSSAEVVDTFVTEKSSETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSV 415
           P+ ++A+D   S AE V   V EK  ++ RF V +G P+PFGAT+ + GVNF+++S +SV
Sbjct: 39  PISISAKDRRISEAENVA--VVEKPLKSDRFLVSDGLPSPFGATVREDGVNFSVYSANSV 96

Query: 416 SATLCLITISDLQQN 460
           SAT+CLI++SDL+QN
Sbjct: 97  SATICLISLSDLRQN 111


>ref|XP_006411237.1| hypothetical protein EUTSA_v10016275mg [Eutrema salsugineum]
           gi|557112406|gb|ESQ52690.1| hypothetical protein
           EUTSA_v10016275mg [Eutrema salsugineum]
          Length = 787

 Score = 75.5 bits (184), Expect = 7e-12
 Identities = 37/75 (49%), Positives = 57/75 (76%)
 Frame = +2

Query: 236 PLMVNARDTTTSSAEVVDTFVTEKSSETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSV 415
           PL+++A+D  +  AE V   V EK  ++ RF + +G P+PFGAT+ + GVNF+++S +SV
Sbjct: 43  PLLLSAKDRRSVEAENVA--VAEKPPKSERFLISDGLPSPFGATVRNDGVNFSVYSANSV 100

Query: 416 SATLCLITISDLQQN 460
           SAT+CLI++SDL+QN
Sbjct: 101 SATICLISLSDLRQN 115


>ref|XP_002879833.1| ATISA1/ISA1 [Arabidopsis lyrata subsp. lyrata]
           gi|297325672|gb|EFH56092.1| ATISA1/ISA1 [Arabidopsis
           lyrata subsp. lyrata]
          Length = 783

 Score = 75.1 bits (183), Expect = 9e-12
 Identities = 37/75 (49%), Positives = 55/75 (73%)
 Frame = +2

Query: 236 PLMVNARDTTTSSAEVVDTFVTEKSSETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSV 415
           P+ ++A+   +S AE +   V EK  ++ RF V  G P+PFGAT+ D GVNF+++S +SV
Sbjct: 39  PISISAKARRSSEAESIA--VVEKPLKSERFNVSNGLPSPFGATVRDDGVNFSVYSTNSV 96

Query: 416 SATLCLITISDLQQN 460
           SAT+CLI++SDL+QN
Sbjct: 97  SATICLISLSDLRQN 111


>gb|EMJ26281.1| hypothetical protein PRUPE_ppa025097mg, partial [Prunus persica]
          Length = 777

 Score = 74.7 bits (182), Expect = 1e-11
 Identities = 35/75 (46%), Positives = 55/75 (73%)
 Frame = +2

Query: 236 PLMVNARDTTTSSAEVVDTFVTEKSSETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSV 415
           P++V       +  +VV+T V ++  +  RF+V +G+P+PFGAT  DGGVNFA++S ++V
Sbjct: 6   PVLVVKASNGGAGTDVVETAVVDRP-KLRRFQVSQGHPSPFGATTRDGGVNFAVYSANAV 64

Query: 416 SATLCLITISDLQQN 460
           S TLCLIT+SDL+++
Sbjct: 65  SVTLCLITLSDLEED 79


>ref|NP_181522.1| isoamylase 1 [Arabidopsis thaliana]
           gi|75096955|sp|O04196.1|ISOA1_ARATH RecName:
           Full=Isoamylase 1, chloroplastic; Short=AtISA1; Flags:
           Precursor gi|2088646|gb|AAB95278.1| putative isoamylase
           [Arabidopsis thaliana] gi|23306386|gb|AAN17420.1|
           putative isoamylase [Arabidopsis thaliana]
           gi|34098817|gb|AAQ56791.1| At2g39930 [Arabidopsis
           thaliana] gi|330254658|gb|AEC09752.1| isoamylase 1
           [Arabidopsis thaliana]
          Length = 783

 Score = 73.6 bits (179), Expect = 3e-11
 Identities = 35/75 (46%), Positives = 56/75 (74%)
 Frame = +2

Query: 236 PLMVNARDTTTSSAEVVDTFVTEKSSETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSV 415
           P+ ++A+D  ++ AE +   V EK  ++ RF + +G P+PFG T+ D GVNF+++S +SV
Sbjct: 39  PISISAKDRRSNEAENIA--VVEKPLKSDRFFISDGLPSPFGPTVRDDGVNFSVYSTNSV 96

Query: 416 SATLCLITISDLQQN 460
           SAT+CLI++SDL+QN
Sbjct: 97  SATICLISLSDLRQN 111


>ref|XP_002529900.1| isoamylase, putative [Ricinus communis] gi|223530627|gb|EEF32503.1|
           isoamylase, putative [Ricinus communis]
          Length = 795

 Score = 73.2 bits (178), Expect = 3e-11
 Identities = 34/67 (50%), Positives = 47/67 (70%)
 Frame = +2

Query: 260 TTTSSAEVVDTFVTEKSSETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSVSATLCLIT 439
           T  +  + V+T V     +  R++V EG+P PFGAT+ DGGVNFAI+S  +VSA+LCLI+
Sbjct: 57  TKAARRDEVETAVVVDKPKFRRYQVSEGHPAPFGATIADGGVNFAIYSSDAVSASLCLIS 116

Query: 440 ISDLQQN 460
           + DL QN
Sbjct: 117 LDDLTQN 123


>ref|XP_004242868.1| PREDICTED: isoamylase 1, chloroplastic-like [Solanum lycopersicum]
          Length = 787

 Score = 72.4 bits (176), Expect = 6e-11
 Identities = 38/73 (52%), Positives = 48/73 (65%), Gaps = 1/73 (1%)
 Frame = +2

Query: 242 MVNARDTTTSSAEVVDTFVTEKSS-ETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSVS 418
           +VNA     ++       V EKS+ E  RFEV  G P PFGAT  DGGVNFA+FS ++ +
Sbjct: 41  VVNAAVHRLAAKTAASAVVVEKSTTERCRFEVLSGKPLPFGATATDGGVNFAVFSRNATA 100

Query: 419 ATLCLITISDLQQ 457
           ATLCLIT+SDL +
Sbjct: 101 ATLCLITLSDLPE 113


>ref|XP_004163012.1| PREDICTED: isoamylase 1, chloroplastic-like [Cucumis sativus]
          Length = 810

 Score = 71.6 bits (174), Expect = 1e-10
 Identities = 37/67 (55%), Positives = 48/67 (71%)
 Frame = +2

Query: 260 TTTSSAEVVDTFVTEKSSETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSVSATLCLIT 439
           T+ + AE V+       S   RFEVF+G PTPFGAT  + G+NFAI+S ++VSATLCLI 
Sbjct: 71  TSVAVAEEVEV------SNLNRFEVFDGTPTPFGATAREDGINFAIYSANAVSATLCLIH 124

Query: 440 ISDLQQN 460
           +SDLQ+N
Sbjct: 125 LSDLQEN 131


>ref|XP_004149676.1| PREDICTED: isoamylase 1, chloroplastic-like [Cucumis sativus]
          Length = 810

 Score = 71.6 bits (174), Expect = 1e-10
 Identities = 37/67 (55%), Positives = 48/67 (71%)
 Frame = +2

Query: 260 TTTSSAEVVDTFVTEKSSETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSVSATLCLIT 439
           T+ + AE V+       S   RFEVF+G PTPFGAT  + G+NFAI+S ++VSATLCLI 
Sbjct: 71  TSVAVAEEVEV------SNLNRFEVFDGTPTPFGATAREDGINFAIYSANAVSATLCLIH 124

Query: 440 ISDLQQN 460
           +SDLQ+N
Sbjct: 125 LSDLQEN 131


>ref|XP_003532455.2| PREDICTED: isoamylase 1, chloroplastic-like [Glycine max]
          Length = 798

 Score = 71.2 bits (173), Expect = 1e-10
 Identities = 41/99 (41%), Positives = 55/99 (55%)
 Frame = +2

Query: 164 TTRISSPFTSIISTGHLQISRTNPPLMVNARDTTTSSAEVVDTFVTEKSSETMRFEVFEG 343
           T R+S   T  +S  H    +TN  ++ + R          +T V EK      F+   G
Sbjct: 32  TVRVSVALTERLSQHH----KTNS-IIPSTRAAANGGGFETETAVVEKPQLKTLFQASRG 86

Query: 344 YPTPFGATLCDGGVNFAIFSGHSVSATLCLITISDLQQN 460
           YP+PFGAT+ DGGVNFAI S +++SATLC  T+SD Q N
Sbjct: 87  YPSPFGATVRDGGVNFAISSLNALSATLCFFTLSDFQNN 125


>gb|EXC12839.1| Isoamylase 1 [Morus notabilis]
          Length = 805

 Score = 70.9 bits (172), Expect = 2e-10
 Identities = 35/63 (55%), Positives = 46/63 (73%)
 Frame = +2

Query: 272 SAEVVDTFVTEKSSETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSVSATLCLITISDL 451
           SAEV    V +   +  RF+V  G P P GATL DGGVNFA+F+G++V ATLCL+++SDL
Sbjct: 59  SAEVA---VVDGGPQLSRFQVSRGSPVPLGATLRDGGVNFAVFAGNAVKATLCLMSLSDL 115

Query: 452 QQN 460
           +QN
Sbjct: 116 EQN 118


>gb|AAY89378.1| isoamylase isoform 1 [Nicotiana langsdorffii x Nicotiana sanderae]
          Length = 264

 Score = 70.9 bits (172), Expect = 2e-10
 Identities = 37/69 (53%), Positives = 47/69 (68%)
 Frame = +2

Query: 251 ARDTTTSSAEVVDTFVTEKSSETMRFEVFEGYPTPFGATLCDGGVNFAIFSGHSVSATLC 430
           A+  T ++A VV+    +   E  RF+V  G P PFGAT  DGGVNFA+FS ++ SATLC
Sbjct: 27  AKAETAATAVVVEE---KPMMERRRFQVLPGKPLPFGATATDGGVNFAVFSSNATSATLC 83

Query: 431 LITISDLQQ 457
           LIT+SDL Q
Sbjct: 84  LITLSDLPQ 92