BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Achyranthes22_contig00056845
         (298 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002272995.1| PREDICTED: leucoanthocyanidin dioxygenase [V...   168   6e-40
ref|XP_006356460.1| PREDICTED: protein SRG1-like [Solanum tubero...   168   8e-40
ref|XP_004164842.1| PREDICTED: leucoanthocyanidin dioxygenase-li...   166   2e-39
gb|EMJ19393.1| hypothetical protein PRUPE_ppa007719mg [Prunus pe...   166   4e-39
ref|XP_003519399.2| PREDICTED: leucoanthocyanidin dioxygenase-li...   165   7e-39
ref|XP_004150750.1| PREDICTED: leucoanthocyanidin dioxygenase-li...   164   1e-38
ref|XP_004235243.1| PREDICTED: leucoanthocyanidin dioxygenase-li...   163   3e-38
ref|XP_006486309.1| PREDICTED: leucoanthocyanidin dioxygenase-li...   162   3e-38
gb|AFK44122.1| unknown [Lotus japonicus]                              162   6e-38
ref|XP_003544081.1| PREDICTED: leucoanthocyanidin dioxygenase-li...   162   6e-38
gb|EOY17762.1| 2-oxoglutarate (2OG) and Fe(II)-dependent oxygena...   161   1e-37
gb|EXB88398.1| Leucoanthocyanidin dioxygenase [Morus notabilis]       160   2e-37
ref|XP_002323582.1| hypothetical protein POPTR_0016s12440g [Popu...   160   2e-37
ref|XP_002536454.1| Leucoanthocyanidin dioxygenase, putative [Ri...   159   5e-37
gb|AFK40747.1| unknown [Medicago truncatula]                          158   8e-37
ref|XP_003616646.1| 1-aminocyclopropane-1-carboxylate oxidase [M...   158   8e-37
ref|NP_001276239.1| uncharacterized protein LOC100801693 [Glycin...   158   8e-37
ref|XP_003600918.1| Leucoanthocyanidin dioxygenase [Medicago tru...   158   8e-37
ref|XP_002533635.1| Leucoanthocyanidin dioxygenase, putative [Ri...   157   1e-36
ref|XP_002312162.1| oxidoreductase family protein [Populus trich...   157   1e-36

>ref|XP_002272995.1| PREDICTED: leucoanthocyanidin dioxygenase [Vitis vinifera]
           gi|147769193|emb|CAN59837.1| hypothetical protein
           VITISV_039830 [Vitis vinifera]
           gi|297736599|emb|CBI25470.3| unnamed protein product
           [Vitis vinifera]
          Length = 359

 Score =  168 bits (426), Expect = 6e-40
 Identities = 75/99 (75%), Positives = 87/99 (87%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLGVEKGAILDWSDYYFLHY PS+LKDHNKWP  P   RE+IE+Y ++VV+L  +LM
Sbjct: 126 YGSRLGVEKGAILDWSDYYFLHYRPSSLKDHNKWPSPPLALREVIEEYGKQVVELAGKLM 185

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
            V SINLGL+E+YLQNAFGG++ GACLRVNFYP+CPQPD
Sbjct: 186 KVLSINLGLKEEYLQNAFGGDDIGACLRVNFYPRCPQPD 224


>ref|XP_006356460.1| PREDICTED: protein SRG1-like [Solanum tuberosum]
          Length = 356

 Score =  168 bits (425), Expect = 8e-40
 Identities = 76/99 (76%), Positives = 85/99 (85%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLGVEKGAILDWSDYYFLHYLP  LKDHNKWP  P   R+++E+YS++VV  G RLM
Sbjct: 115 YGSRLGVEKGAILDWSDYYFLHYLPGYLKDHNKWPALPLSLRKVMEEYSKQVVNFGGRLM 174

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
            +FS NLGL+ED LQNAFGGE+ GACLRVNFYPKCPQPD
Sbjct: 175 KIFSKNLGLKEDVLQNAFGGEDIGACLRVNFYPKCPQPD 213


>ref|XP_004164842.1| PREDICTED: leucoanthocyanidin dioxygenase-like [Cucumis sativus]
          Length = 357

 Score =  166 bits (421), Expect = 2e-39
 Identities = 73/99 (73%), Positives = 87/99 (87%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLGV+KGAILDWSDY+FLHYLPS+LKDH+KWP  P + REM E+Y  EVVK+G +L+
Sbjct: 128 YGSRLGVQKGAILDWSDYFFLHYLPSHLKDHSKWPAIPDFIREMTEEYGEEVVKVGGQLL 187

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
            + S+NLGL+E YLQNAFGGEE GACLRVN+YPKCPQP+
Sbjct: 188 KLLSLNLGLQEGYLQNAFGGEEVGACLRVNYYPKCPQPE 226


>gb|EMJ19393.1| hypothetical protein PRUPE_ppa007719mg [Prunus persica]
          Length = 358

 Score =  166 bits (419), Expect = 4e-39
 Identities = 72/99 (72%), Positives = 86/99 (86%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLGVEKGAILDWSDYYFLHYLP +LKDH+KWP  P Y R++I++Y +EVVKL  R++
Sbjct: 124 YGSRLGVEKGAILDWSDYYFLHYLPLSLKDHDKWPALPGYARQVIDEYGKEVVKLCGRIL 183

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
            + S+NLGLE+D+LQ  FGGE+ GACLRVNFYPKCPQPD
Sbjct: 184 KILSLNLGLEQDFLQKGFGGEDIGACLRVNFYPKCPQPD 222


>ref|XP_003519399.2| PREDICTED: leucoanthocyanidin dioxygenase-like [Glycine max]
          Length = 378

 Score =  165 bits (417), Expect = 7e-39
 Identities = 75/99 (75%), Positives = 85/99 (85%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLG+EKGAILDWSDYY+LHYLP +LKDHNKWP QP   RE+ ++Y REVVKL  RLM
Sbjct: 145 YGSRLGIEKGAILDWSDYYYLHYLPLSLKDHNKWPTQPPSCREVCDEYGREVVKLCGRLM 204

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
            V SINLGLEED L+ AFGGE+ GACLRVNFYPKCP+P+
Sbjct: 205 KVLSINLGLEEDVLEKAFGGEDVGACLRVNFYPKCPRPE 243


>ref|XP_004150750.1| PREDICTED: leucoanthocyanidin dioxygenase-like [Cucumis sativus]
           gi|449515609|ref|XP_004164841.1| PREDICTED:
           leucoanthocyanidin dioxygenase-like [Cucumis sativus]
          Length = 361

 Score =  164 bits (414), Expect = 1e-38
 Identities = 72/99 (72%), Positives = 86/99 (86%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLGV+KGAILDWSDY+FLHYLPS+LKDHNKWP  P   RE+ E+Y  EVVK+G +L+
Sbjct: 128 YGSRLGVQKGAILDWSDYFFLHYLPSHLKDHNKWPVIPNSIRELTEEYGEEVVKVGGKLL 187

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
            + S+NLGL+E YLQNAFGGE+ GACLRVN+YPKCPQP+
Sbjct: 188 KLLSLNLGLQEGYLQNAFGGEDVGACLRVNYYPKCPQPE 226


>ref|XP_004235243.1| PREDICTED: leucoanthocyanidin dioxygenase-like [Solanum
           lycopersicum]
          Length = 357

 Score =  163 bits (412), Expect = 3e-38
 Identities = 74/99 (74%), Positives = 84/99 (84%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLGVEK AILDWSDYYFLHYLP+ LKD+NKWP  P+  RE++E+YS++VV  G RLM
Sbjct: 116 YGSRLGVEKDAILDWSDYYFLHYLPAYLKDYNKWPALPSSLREVMEEYSKQVVNFGGRLM 175

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
            + S NLGL ED LQNAFGGE+ GACLRVNFYPKCPQPD
Sbjct: 176 KILSENLGLNEDVLQNAFGGEDIGACLRVNFYPKCPQPD 214


>ref|XP_006486309.1| PREDICTED: leucoanthocyanidin dioxygenase-like isoform X1 [Citrus
           sinensis] gi|568865909|ref|XP_006486310.1| PREDICTED:
           leucoanthocyanidin dioxygenase-like isoform X2 [Citrus
           sinensis]
          Length = 361

 Score =  162 bits (411), Expect = 3e-38
 Identities = 74/99 (74%), Positives = 83/99 (83%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLGVEKGAILDWSDYYFLHY PS+LKD NKWP  P   RE+I++Y +E+V+L  RLM
Sbjct: 128 YGSRLGVEKGAILDWSDYYFLHYRPSSLKDFNKWPATPASCREVIDEYCKELVRLSGRLM 187

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
            V SINLG+ ED LQNAFGG+  GACLRVNFYPKCPQPD
Sbjct: 188 KVLSINLGISEDRLQNAFGGDNIGACLRVNFYPKCPQPD 226


>gb|AFK44122.1| unknown [Lotus japonicus]
          Length = 367

 Score =  162 bits (409), Expect = 6e-38
 Identities = 73/99 (73%), Positives = 85/99 (85%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLGVEKGAILDWSDYY+LHYLP +LKDHNKWP  P   RE+ ++Y RE+VKL  RLM
Sbjct: 134 YGSRLGVEKGAILDWSDYYYLHYLPCSLKDHNKWPSLPFGCREVFDEYGRELVKLCGRLM 193

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
            + SINLGLEE+ LQ+AFGGE+ GACLRVNFYPKCP+P+
Sbjct: 194 KILSINLGLEEEILQSAFGGEDIGACLRVNFYPKCPKPE 232


>ref|XP_003544081.1| PREDICTED: leucoanthocyanidin dioxygenase-like [Glycine max]
          Length = 361

 Score =  162 bits (409), Expect = 6e-38
 Identities = 73/99 (73%), Positives = 85/99 (85%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLG+EKGAILDWSDYY+LHYLP +LKD+NKWP QP   RE+ ++Y RE+VKL  RLM
Sbjct: 128 YGSRLGIEKGAILDWSDYYYLHYLPLSLKDNNKWPSQPPSCREVCDEYGRELVKLCGRLM 187

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
            V SINLGLEED LQ AFGGE+ GAC+RVNFYPKCP+P+
Sbjct: 188 KVLSINLGLEEDALQKAFGGEDVGACMRVNFYPKCPRPE 226


>gb|EOY17762.1| 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily
           protein [Theobroma cacao]
          Length = 359

 Score =  161 bits (407), Expect = 1e-37
 Identities = 73/99 (73%), Positives = 82/99 (82%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLG+EKGAILDWSDYYFLHYLP  LKD+ KWP  P   RE+I++Y +EVVKL  R+M
Sbjct: 126 YGSRLGIEKGAILDWSDYYFLHYLPLTLKDYKKWPTSPDSCREVIDEYGKEVVKLCGRIM 185

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
            V S+NLGL ED+LQN FGGE  GACLRVNFYPKCPQPD
Sbjct: 186 KVLSMNLGLREDHLQNEFGGENFGACLRVNFYPKCPQPD 224


>gb|EXB88398.1| Leucoanthocyanidin dioxygenase [Morus notabilis]
          Length = 360

 Score =  160 bits (405), Expect = 2e-37
 Identities = 73/99 (73%), Positives = 85/99 (85%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLGV+KGAILDWSDYYFLHYLPS LKD+ KWP  P   RE+I++Y REVVKL  RL+
Sbjct: 127 YGSRLGVQKGAILDWSDYYFLHYLPSCLKDYKKWPHLPPNCREVIDEYGREVVKLCGRLL 186

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
            V S+NLGL+E +LQNAFGGE+ GACLRVN+YPKCPQP+
Sbjct: 187 RVLSVNLGLKEHFLQNAFGGEDIGACLRVNYYPKCPQPE 225


>ref|XP_002323582.1| hypothetical protein POPTR_0016s12440g [Populus trichocarpa]
           gi|222868212|gb|EEF05343.1| hypothetical protein
           POPTR_0016s12440g [Populus trichocarpa]
          Length = 367

 Score =  160 bits (404), Expect = 2e-37
 Identities = 72/100 (72%), Positives = 85/100 (85%), Gaps = 1/100 (1%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLGVEKGA LDWSDY+FLHY+P +L++ NKWP  P   RE++ +Y REVVKLG +LM
Sbjct: 128 YGSRLGVEKGATLDWSDYFFLHYMPVSLRNQNKWPATPASCRELVAEYGREVVKLGGKLM 187

Query: 181 NVFSINLGLEEDYLQNAFGGEEN-GACLRVNFYPKCPQPD 297
             FS+NLGLEED+L +AFGGEEN GACLRVN+YPKCPQPD
Sbjct: 188 KAFSMNLGLEEDFLLDAFGGEENVGACLRVNYYPKCPQPD 227


>ref|XP_002536454.1| Leucoanthocyanidin dioxygenase, putative [Ricinus communis]
           gi|223519641|gb|EEF25929.1| Leucoanthocyanidin
           dioxygenase, putative [Ricinus communis]
          Length = 293

 Score =  159 bits (401), Expect = 5e-37
 Identities = 73/99 (73%), Positives = 84/99 (84%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLGVEKGAILDWSDYYFLHYLP +LKD+NKWP  P   RE+I +Y ++V++L  R+M
Sbjct: 136 YGSRLGVEKGAILDWSDYYFLHYLPLSLKDYNKWPRLPPNCREVINEYGKQVLELCGRIM 195

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
            V SINLGLEED LQ+AFGG+  GACLRVNFYPKCPQPD
Sbjct: 196 KVLSINLGLEEDRLQDAFGGKNVGACLRVNFYPKCPQPD 234


>gb|AFK40747.1| unknown [Medicago truncatula]
          Length = 353

 Score =  158 bits (399), Expect = 8e-37
 Identities = 70/99 (70%), Positives = 86/99 (86%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLGV+KGAILDWSDYY+LHYLP +LKD+NKWP  P   RE+ ++Y  E+VKL +RLM
Sbjct: 125 YGSRLGVKKGAILDWSDYYYLHYLPLSLKDYNKWPSLPPSCREVFDEYGIELVKLCERLM 184

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
            V SINLGLEE++LQNAFGGE+ GAC+RVN++PKCPQP+
Sbjct: 185 KVLSINLGLEENFLQNAFGGEDIGACIRVNYFPKCPQPE 223


>ref|XP_003616646.1| 1-aminocyclopropane-1-carboxylate oxidase [Medicago truncatula]
           gi|355517981|gb|AES99604.1|
           1-aminocyclopropane-1-carboxylate oxidase [Medicago
           truncatula]
          Length = 358

 Score =  158 bits (399), Expect = 8e-37
 Identities = 70/99 (70%), Positives = 86/99 (86%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLGV+KGAILDWSDYY+LHYLP +LKD+NKWP  P   RE+ ++Y  E+VKL +RLM
Sbjct: 125 YGSRLGVKKGAILDWSDYYYLHYLPLSLKDYNKWPSLPPSCREVFDEYGIELVKLCERLM 184

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
            V SINLGLEE++LQNAFGGE+ GAC+RVN++PKCPQP+
Sbjct: 185 KVLSINLGLEENFLQNAFGGEDIGACIRVNYFPKCPQPE 223


>ref|NP_001276239.1| uncharacterized protein LOC100801693 [Glycine max]
           gi|255636356|gb|ACU18517.1| unknown [Glycine max]
          Length = 361

 Score =  158 bits (399), Expect = 8e-37
 Identities = 71/99 (71%), Positives = 83/99 (83%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLG+EKGAILDWSDYYFLHYLP +LKD+NKWP  P   RE+++ Y RE+V+L  RLM
Sbjct: 128 YGSRLGIEKGAILDWSDYYFLHYLPFSLKDYNKWPASPPSCREVLDGYGRELVRLCGRLM 187

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
             FSINLGL+E  LQN FGGE+ GACLRVNFYPKCP+P+
Sbjct: 188 KAFSINLGLDEKILQNDFGGEDIGACLRVNFYPKCPRPE 226


>ref|XP_003600918.1| Leucoanthocyanidin dioxygenase [Medicago truncatula]
           gi|355489966|gb|AES71169.1| Leucoanthocyanidin
           dioxygenase [Medicago truncatula]
          Length = 359

 Score =  158 bits (399), Expect = 8e-37
 Identities = 71/99 (71%), Positives = 82/99 (82%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLGVEKGAILDWSDYYFLHYLP ++KD NKWP  P   RE+ ++Y +E+VKL  RLM
Sbjct: 126 YGSRLGVEKGAILDWSDYYFLHYLPVSVKDCNKWPASPQSCREVFDEYGKELVKLSGRLM 185

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
              S+NLGLEE  LQNAFGGEE GAC+RVNFYPKCP+P+
Sbjct: 186 KALSLNLGLEEKILQNAFGGEEIGACMRVNFYPKCPRPE 224


>ref|XP_002533635.1| Leucoanthocyanidin dioxygenase, putative [Ricinus communis]
           gi|223526473|gb|EEF28746.1| Leucoanthocyanidin
           dioxygenase, putative [Ricinus communis]
          Length = 362

 Score =  157 bits (398), Expect = 1e-36
 Identities = 71/100 (71%), Positives = 83/100 (83%), Gaps = 1/100 (1%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLGVE+G  LDWSDY+FLHY+P++L++H KWP  P   RE++ +Y  EVVKLG RLM
Sbjct: 129 YGSRLGVEQGVTLDWSDYFFLHYMPASLRNHQKWPAIPASCRELVAEYGSEVVKLGGRLM 188

Query: 181 NVFSINLGLEEDYLQNAFGGEEN-GACLRVNFYPKCPQPD 297
            VFS NLGLEED LQN FGG++N GACLRVNFYPKCPQPD
Sbjct: 189 RVFSTNLGLEEDRLQNTFGGDDNIGACLRVNFYPKCPQPD 228


>ref|XP_002312162.1| oxidoreductase family protein [Populus trichocarpa]
           gi|222851982|gb|EEE89529.1| oxidoreductase family
           protein [Populus trichocarpa]
          Length = 358

 Score =  157 bits (398), Expect = 1e-36
 Identities = 73/99 (73%), Positives = 83/99 (83%)
 Frame = +1

Query: 1   YGSRLGVEKGAILDWSDYYFLHYLPSNLKDHNKWPGQPTYHREMIEQYSREVVKLGKRLM 180
           YGSRLGVEKGAILDWSDYYFLHYLPS LKD +KWP  P   RE++++Y +++VKL  RLM
Sbjct: 125 YGSRLGVEKGAILDWSDYYFLHYLPSTLKDCSKWPTIPADCREVLDEYGKQLVKLCGRLM 184

Query: 181 NVFSINLGLEEDYLQNAFGGEENGACLRVNFYPKCPQPD 297
            + SINLGL E+ LQNAFGGE  GACLRVNFYPKCPQPD
Sbjct: 185 KILSINLGLGEERLQNAFGGENIGACLRVNFYPKCPQPD 223