BLASTX nr result
ID: Achyranthes22_contig00023103
seq
BLASTX 2.2.25 [Feb-01-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Achyranthes22_contig00023103 (1246 letters) Database: ./nr 37,332,560 sequences; 13,225,080,153 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|XP_002522423.1| sur2 hydroxylase/desaturase, putative [Ricin... 227 e-101 ref|XP_006363216.1| PREDICTED: sphinganine C(4)-monooxygenase 1-... 228 2e-99 gb|ACQ90236.1| sphingolipid C4 hydroxylase [Helianthus annuus] 229 2e-99 gb|ABO92998.1| putative sterol desaturase [Solanum tuberosum] 228 5e-99 ref|XP_004238795.1| PREDICTED: sphinganine C(4)-monooxygenase 2-... 226 7e-99 gb|ABO93014.1| putative sterol desaturase [Solanum tuberosum] 226 9e-99 gb|EXB38329.1| Sphingoid base hydroxylase 2 [Morus notabilis] 221 3e-98 ref|XP_002275229.1| PREDICTED: sphingoid base hydroxylase 2 [Vit... 221 4e-98 gb|EOY01945.1| Sphingoid base hydroxylase 2 [Theobroma cacao] 224 6e-98 ref|XP_006390989.1| hypothetical protein EUTSA_v10019024mg [Eutr... 219 6e-98 gb|EMJ27194.1| hypothetical protein PRUPE_ppa010258mg [Prunus pe... 219 7e-98 ref|NP_177122.2| sphingoid base hydroxylase 1 [Arabidopsis thali... 221 2e-97 gb|AAG12703.1|AC021046_4 acid phosphatase, putative; 5376-6903 [... 221 2e-97 ref|XP_006304118.1| hypothetical protein CARUB_v10010052mg [Caps... 223 6e-97 ref|XP_002888727.1| hypothetical protein ARALYDRAFT_476099 [Arab... 220 1e-96 ref|NP_563944.1| sphingoid base hydroxylase 2 [Arabidopsis thali... 223 1e-96 gb|AAF43928.1|AC012188_5 Contains similarity to acid phosphatase... 223 1e-96 ref|XP_006302713.1| hypothetical protein CARUB_v10020825mg [Caps... 217 2e-96 ref|XP_006417030.1| hypothetical protein EUTSA_v10008529mg [Eutr... 221 2e-96 ref|XP_004297306.1| PREDICTED: sphinganine C(4)-monooxygenase 2-... 219 2e-96 >ref|XP_002522423.1| sur2 hydroxylase/desaturase, putative [Ricinus communis] gi|223538308|gb|EEF39915.1| sur2 hydroxylase/desaturase, putative [Ricinus communis] Length = 258 Score = 227 bits (578), Expect(2) = e-101 Identities = 107/150 (71%), Positives = 123/150 (82%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y +G D YRLH++QDEDEKNL+SKSTV+KGVLLQQ +QAV AIILF VTG + A Sbjct: 24 GLYIGLGYFDNYRLHTKQDEDEKNLVSKSTVVKGVLLQQAIQAVVAIILFTVTGDDSETA 83 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 Q + +L Q AM+VLDTWQYFMHRYMHHNKFLY+HIHSQHHRL+VPYA+GALYN Sbjct: 84 MDQKPSLMILARQFVTAMLVLDTWQYFMHRYMHHNKFLYKHIHSQHHRLVVPYAFGALYN 143 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGL+LDTIGGALSFLL+GMSPR FF Sbjct: 144 HPVEGLLLDTIGGALSFLLSGMSPRASVFF 173 Score = 171 bits (432), Expect(2) = e-101 Identities = 72/85 (84%), Positives = 82/85 (96%) Frame = -2 Query: 627 SIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWDK 448 S+FFFSFATIKTVDDHCGLWLPGNLFH+FF+NNTAYHDVHHQLYGSK+NFSQPFF+ WDK Sbjct: 170 SVFFFSFATIKTVDDHCGLWLPGNLFHVFFKNNTAYHDVHHQLYGSKYNFSQPFFVMWDK 229 Query: 447 ILGTYMPYTLEHREGGGYEARPKED 373 ILGTYMPY+LE R+GGG+EARP ++ Sbjct: 230 ILGTYMPYSLEKRDGGGFEARPAKE 254 >ref|XP_006363216.1| PREDICTED: sphinganine C(4)-monooxygenase 1-like [Solanum tuberosum] Length = 269 Score = 228 bits (581), Expect(2) = 2e-99 Identities = 105/150 (70%), Positives = 127/150 (84%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y M+G++D YRLHS++DEDEKNL+SK V+KGVLLQQ VQA A +LFAVTG + Sbjct: 35 GLYLMLGTMDNYRLHSKKDEDEKNLVSKKEVVKGVLLQQIVQAAVATVLFAVTGDDGESG 94 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 ++T+ FVLG Q+F AM++LDTWQYFMHRYMH NKFLY+HIH+QHHRLIVPYA+GALYN Sbjct: 95 GDRHTSIFVLGRQLFVAMLMLDTWQYFMHRYMHQNKFLYKHIHAQHHRLIVPYAFGALYN 154 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGLILDTIGGAL+FL++GMSPR FF Sbjct: 155 HPLEGLILDTIGGALAFLVSGMSPRTSIFF 184 Score = 163 bits (412), Expect(2) = 2e-99 Identities = 69/86 (80%), Positives = 80/86 (93%) Frame = -2 Query: 630 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWD 451 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFF+NN+AYHD+HHQLYG+K+N+SQPFF+ WD Sbjct: 180 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFKNNSAYHDIHHQLYGTKYNYSQPFFVTWD 239 Query: 450 KILGTYMPYTLEHREGGGYEARPKED 373 +ILGTYMPY LE R GG+EA+P +D Sbjct: 240 RILGTYMPYELERRPEGGFEAKPVKD 265 >gb|ACQ90236.1| sphingolipid C4 hydroxylase [Helianthus annuus] Length = 258 Score = 229 bits (584), Expect(2) = 2e-99 Identities = 108/150 (72%), Positives = 126/150 (84%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y + GS ++YRLHS+++ED+KNL+SK TV+KGVLLQQ +QAV AIILF VTGS P Sbjct: 24 GFYVLFGSSEKYRLHSKKEEDDKNLVSKKTVVKGVLLQQAIQAVVAIILFTVTGSDESPT 83 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 ++ + F LG Q F AM+VLDTWQYFMHRYMH NKFLYRHIHSQHHRLIVPYAYGALYN Sbjct: 84 TNGSLSVFDLGRQFFTAMVVLDTWQYFMHRYMHQNKFLYRHIHSQHHRLIVPYAYGALYN 143 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGL+LDTIGGAL+FLL+GMSPR FF Sbjct: 144 HPLEGLLLDTIGGALAFLLSGMSPRASIFF 173 Score = 162 bits (409), Expect(2) = 2e-99 Identities = 69/82 (84%), Positives = 77/82 (93%) Frame = -2 Query: 627 SIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWDK 448 SIFFFSFATIKTVDDHCGLWLPGNLFH+FF+NN+AYHDVHHQLYG+K+N+SQPFF WDK Sbjct: 170 SIFFFSFATIKTVDDHCGLWLPGNLFHVFFQNNSAYHDVHHQLYGTKYNYSQPFFNVWDK 229 Query: 447 ILGTYMPYTLEHREGGGYEARP 382 ILGT+MPYTLE RE GG+E RP Sbjct: 230 ILGTHMPYTLEKRENGGFEVRP 251 >gb|ABO92998.1| putative sterol desaturase [Solanum tuberosum] Length = 269 Score = 228 bits (580), Expect(2) = 5e-99 Identities = 105/150 (70%), Positives = 127/150 (84%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y M+G++D YRLHS++DEDEKNL+SK V+KGVLLQQ VQA A +LFAVTG + Sbjct: 35 GLYLMLGNMDNYRLHSKKDEDEKNLVSKKEVVKGVLLQQIVQAAVATVLFAVTGDDGESG 94 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 ++T+ FVLG Q+F AM++LDTWQYFMHRYMH NKFLY+HIH+QHHRLIVPYA+GALYN Sbjct: 95 GDRHTSIFVLGRQLFIAMLMLDTWQYFMHRYMHQNKFLYKHIHAQHHRLIVPYAFGALYN 154 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGLILDTIGGAL+FL++GMSPR FF Sbjct: 155 HPLEGLILDTIGGALAFLVSGMSPRTSIFF 184 Score = 162 bits (409), Expect(2) = 5e-99 Identities = 69/86 (80%), Positives = 79/86 (91%) Frame = -2 Query: 630 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWD 451 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFF+NN+AYHD+HHQLYG+K+N+SQPFF+ WD Sbjct: 180 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFKNNSAYHDIHHQLYGTKYNYSQPFFVTWD 239 Query: 450 KILGTYMPYTLEHREGGGYEARPKED 373 ILGTYMPY LE R GG+EA+P +D Sbjct: 240 MILGTYMPYELERRPEGGFEAKPVKD 265 >ref|XP_004238795.1| PREDICTED: sphinganine C(4)-monooxygenase 2-like [Solanum lycopersicum] Length = 269 Score = 226 bits (576), Expect(2) = 7e-99 Identities = 104/150 (69%), Positives = 125/150 (83%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y M+G++D YRLHS++DEDEKNL+SK V+KGVLLQQ VQA A +LFAVTG + Sbjct: 35 GLYLMLGNMDNYRLHSKKDEDEKNLVSKKEVVKGVLLQQIVQAAVATVLFAVTGDDGESG 94 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 Q+ + FVLG Q+F M++LDTWQYFMHRYMH NKFLY+HIH+QHHRLIVPYA+GALYN Sbjct: 95 GDQHASIFVLGRQLFVGMLMLDTWQYFMHRYMHQNKFLYKHIHAQHHRLIVPYAFGALYN 154 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGLILDTIGGAL+FL++GMSPR FF Sbjct: 155 HPLEGLILDTIGGALAFLVSGMSPRTSIFF 184 Score = 163 bits (412), Expect(2) = 7e-99 Identities = 69/86 (80%), Positives = 80/86 (93%) Frame = -2 Query: 630 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWD 451 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFF+NN+AYHD+HHQLYG+K+N+SQPFF+ WD Sbjct: 180 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFKNNSAYHDIHHQLYGTKYNYSQPFFVTWD 239 Query: 450 KILGTYMPYTLEHREGGGYEARPKED 373 +ILGTYMPY LE R GG+EA+P +D Sbjct: 240 RILGTYMPYELERRPEGGFEAKPVKD 265 >gb|ABO93014.1| putative sterol desaturase [Solanum tuberosum] Length = 269 Score = 226 bits (575), Expect(2) = 9e-99 Identities = 103/150 (68%), Positives = 126/150 (84%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y M+G++D YRLHS++DEDEKNL+SK V+KGVLLQQ VQA A +LFAVTG + Sbjct: 35 GLYLMLGTMDNYRLHSKKDEDEKNLVSKKEVVKGVLLQQIVQAAVATVLFAVTGDDGESG 94 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 ++ + FVLG Q+F AM++LDTWQYFMHRYMH NKFLY+HIH+QHHRLI+PYA+GALYN Sbjct: 95 GDRHASIFVLGRQLFVAMLMLDTWQYFMHRYMHQNKFLYKHIHAQHHRLIIPYAFGALYN 154 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGLILDTIGGAL+FL++GMSPR FF Sbjct: 155 HPLEGLILDTIGGALAFLVSGMSPRTSIFF 184 Score = 163 bits (412), Expect(2) = 9e-99 Identities = 69/86 (80%), Positives = 80/86 (93%) Frame = -2 Query: 630 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWD 451 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFF+NN+AYHD+HHQLYG+K+N+SQPFF+ WD Sbjct: 180 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFKNNSAYHDIHHQLYGTKYNYSQPFFVTWD 239 Query: 450 KILGTYMPYTLEHREGGGYEARPKED 373 +ILGTYMPY LE R GG+EA+P +D Sbjct: 240 RILGTYMPYELERRPEGGFEAKPVKD 265 >gb|EXB38329.1| Sphingoid base hydroxylase 2 [Morus notabilis] Length = 258 Score = 221 bits (564), Expect(2) = 3e-98 Identities = 105/150 (70%), Positives = 123/150 (82%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y ++ S + YRLHS+ DEDEKNL+SK TV+KGVLLQQT+QAV AI+LF VTG+ A Sbjct: 24 GIYALLDSFENYRLHSKIDEDEKNLVSKRTVVKGVLLQQTIQAVVAILLFTVTGNDSGDA 83 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 + + VL Q AM+VLDTWQYFMHRYMHHNKFLY+HIHSQHHRL+VPYA+GALYN Sbjct: 84 AGPKPSLIVLARQFVTAMLVLDTWQYFMHRYMHHNKFLYKHIHSQHHRLVVPYAFGALYN 143 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGL+LDTIGGALSFLL+GMSPR FF Sbjct: 144 HPLEGLLLDTIGGALSFLLSGMSPRASIFF 173 Score = 165 bits (418), Expect(2) = 3e-98 Identities = 70/85 (82%), Positives = 78/85 (91%) Frame = -2 Query: 627 SIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWDK 448 SIFFFSFAT+KTVDDHCGLWLPGNLFH FF NNTAYHDVHHQLYGSK+NFSQPFF+ WD+ Sbjct: 170 SIFFFSFATVKTVDDHCGLWLPGNLFHFFFRNNTAYHDVHHQLYGSKYNFSQPFFVMWDR 229 Query: 447 ILGTYMPYTLEHREGGGYEARPKED 373 ILGTYMPY+L+ R GGG+E RP +D Sbjct: 230 ILGTYMPYSLDKRAGGGFETRPLKD 254 >ref|XP_002275229.1| PREDICTED: sphingoid base hydroxylase 2 [Vitis vinifera] gi|296086379|emb|CBI31968.3| unnamed protein product [Vitis vinifera] Length = 258 Score = 221 bits (562), Expect(2) = 4e-98 Identities = 104/150 (69%), Positives = 123/150 (82%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y +GSL+ YRLHS+++E++KNL+SK TV+KGV+LQQ +QAV AI+LF VTG A Sbjct: 24 GIYIPLGSLENYRLHSKKEEEDKNLVSKGTVVKGVILQQVIQAVVAILLFTVTGDDSGAA 83 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 + + VL Q F AM VLDTWQYFMHRYMHHNKFLYRHIHSQHHRL+VPYA+GALYN Sbjct: 84 AGPKPSLIVLARQFFIAMFVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLVVPYAFGALYN 143 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGL+LDTIGGALSFLL+GMSPR FF Sbjct: 144 HPLEGLLLDTIGGALSFLLSGMSPRASIFF 173 Score = 166 bits (419), Expect(2) = 4e-98 Identities = 71/85 (83%), Positives = 79/85 (92%) Frame = -2 Query: 627 SIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWDK 448 SIFFFSFATIKTVDDHCGLWLPGNLFH+FF NN+AYHD+HHQLYGSK+NFSQPFF+ WDK Sbjct: 170 SIFFFSFATIKTVDDHCGLWLPGNLFHLFFRNNSAYHDIHHQLYGSKYNFSQPFFVMWDK 229 Query: 447 ILGTYMPYTLEHREGGGYEARPKED 373 ILGTYMPY+LE R GGG EARP ++ Sbjct: 230 ILGTYMPYSLEKRAGGGLEARPTKE 254 >gb|EOY01945.1| Sphingoid base hydroxylase 2 [Theobroma cacao] Length = 258 Score = 224 bits (572), Expect(2) = 6e-98 Identities = 104/150 (69%), Positives = 125/150 (83%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y +GS + YRLHS++DEDEKNL+SK TV+KGVLLQQTVQAV AI+LF VTGS + Sbjct: 24 GIYMCLGSFEDYRLHSKKDEDEKNLVSKQTVVKGVLLQQTVQAVVAILLFTVTGSDAGAS 83 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 + Q ++ VL Q AM+V+DTWQYF+HRY HHNKFLYRH+HSQHHRL+VPYA+GALYN Sbjct: 84 AGQPSSLIVLARQFVTAMLVMDTWQYFLHRYFHHNKFLYRHLHSQHHRLVVPYAFGALYN 143 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGL+LDT+GGALSFLL+GMSPR FF Sbjct: 144 HPLEGLLLDTVGGALSFLLSGMSPRTSIFF 173 Score = 161 bits (408), Expect(2) = 6e-98 Identities = 71/86 (82%), Positives = 79/86 (91%) Frame = -2 Query: 630 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWD 451 TSIFFFSFATIKTVDDHCGL LPGNLFHIFF NN+AYHDVHHQLYGSK+NFSQPFF+ WD Sbjct: 169 TSIFFFSFATIKTVDDHCGLLLPGNLFHIFFRNNSAYHDVHHQLYGSKYNFSQPFFIMWD 228 Query: 450 KILGTYMPYTLEHREGGGYEARPKED 373 +ILGTYMPY+LE R GG+EARP ++ Sbjct: 229 RILGTYMPYSLEKRADGGFEARPTKE 254 >ref|XP_006390989.1| hypothetical protein EUTSA_v10019024mg [Eutrema salsugineum] gi|557087423|gb|ESQ28275.1| hypothetical protein EUTSA_v10019024mg [Eutrema salsugineum] Length = 258 Score = 219 bits (559), Expect(2) = 6e-98 Identities = 104/150 (69%), Positives = 123/150 (82%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y + SL+ YRLHS+ +E+EKNL+SKS+V+KGVLLQQ VQAV AI+LF VTGS + Sbjct: 24 GIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLLQQLVQAVVAIVLFTVTGSDAEAD 83 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 + + VL Q AMI+LDTWQYFMHRYMH+NKFLY+HIHSQHHRLIVPYAYGALYN Sbjct: 84 KAREFSLLVLARQFVTAMIILDTWQYFMHRYMHYNKFLYKHIHSQHHRLIVPYAYGALYN 143 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGLILDT+GGALSFL++GMSPR FF Sbjct: 144 HPVEGLILDTVGGALSFLISGMSPRTSIFF 173 Score = 166 bits (421), Expect(2) = 6e-98 Identities = 72/86 (83%), Positives = 81/86 (94%) Frame = -2 Query: 630 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWD 451 TSIFFFSFATIKTVDDHCGLWLPGNLFH+ F+NN+AYHDVHHQLYGSK+NFSQPFF+ WD Sbjct: 169 TSIFFFSFATIKTVDDHCGLWLPGNLFHMVFKNNSAYHDVHHQLYGSKYNFSQPFFVMWD 228 Query: 450 KILGTYMPYTLEHREGGGYEARPKED 373 +ILGTYMPY+LE REGGG EARP ++ Sbjct: 229 RILGTYMPYSLEKREGGGLEARPTKE 254 >gb|EMJ27194.1| hypothetical protein PRUPE_ppa010258mg [Prunus persica] Length = 257 Score = 219 bits (558), Expect(2) = 7e-98 Identities = 105/150 (70%), Positives = 124/150 (82%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y ++ S + YRLH+R+DEDEKNL+SK TV+KGVLLQQT+QA+ AI+LF VTG+ D Sbjct: 24 GIYVVLDSFENYRLHTRKDEDEKNLVSKRTVVKGVLLQQTIQAIVAILLFTVTGND-DGD 82 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 S ++ L Q AMIVLDTWQYFMHRYMHHNKFLY+HIHSQHHRL+VPYA+GALYN Sbjct: 83 SGVKPSFIDLARQFVTAMIVLDTWQYFMHRYMHHNKFLYKHIHSQHHRLVVPYAFGALYN 142 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGL+LDTIGGALSFLL+GMSPR FF Sbjct: 143 HPVEGLLLDTIGGALSFLLSGMSPRASIFF 172 Score = 166 bits (421), Expect(2) = 7e-98 Identities = 71/84 (84%), Positives = 79/84 (94%) Frame = -2 Query: 627 SIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWDK 448 SIFFFSFATIKTVDDHCGLWLPGNLFH+FF NNTAYHDVHHQLYGSK+NFSQPFF+ WD+ Sbjct: 169 SIFFFSFATIKTVDDHCGLWLPGNLFHVFFSNNTAYHDVHHQLYGSKYNFSQPFFVMWDR 228 Query: 447 ILGTYMPYTLEHREGGGYEARPKE 376 ILGT+MPY+LE R GGG+E RPK+ Sbjct: 229 ILGTHMPYSLEKRAGGGFEVRPKK 252 >ref|NP_177122.2| sphingoid base hydroxylase 1 [Arabidopsis thaliana] gi|75248486|sp|Q8VYI1.1|SBH1_ARATH RecName: Full=Sphinganine C(4)-monooxygenase 1; AltName: Full=Sphingoid C4-hydroxylase 1; AltName: Full=Sphingoid base hydroxylase 1 gi|17979535|gb|AAL50102.1| At1g69640/F24J1.22 [Arabidopsis thaliana] gi|23505995|gb|AAN28857.1| At1g69640/F24J1.22 [Arabidopsis thaliana] gi|332196837|gb|AEE34958.1| sphingoid base hydroxylase 1 [Arabidopsis thaliana] Length = 260 Score = 221 bits (562), Expect(2) = 2e-97 Identities = 105/150 (70%), Positives = 123/150 (82%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y + SL+ YRLHS+ +E+EKNL+SKS+V+KGVL+QQ VQAV AI+LF VTGS + Sbjct: 26 GIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLVQQVVQAVVAILLFTVTGSDAEAD 85 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 Q ++ VL Q AMIVLDTWQYFMHRYMH NKFLY+HIHSQHHRLIVPYAYGALYN Sbjct: 86 KAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKFLYKHIHSQHHRLIVPYAYGALYN 145 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGL+LDTIGGALSFL++GMSPR FF Sbjct: 146 HPVEGLLLDTIGGALSFLVSGMSPRTSIFF 175 Score = 164 bits (414), Expect(2) = 2e-97 Identities = 69/86 (80%), Positives = 81/86 (94%) Frame = -2 Query: 630 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWD 451 TSIFFFSFATIKTVDDHCGLWLPGNLFH+ F+NN+AYHD+HHQLYG+K+NFSQPFF+ WD Sbjct: 171 TSIFFFSFATIKTVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWD 230 Query: 450 KILGTYMPYTLEHREGGGYEARPKED 373 +ILGTYMPY+LE RE GG+EARP ++ Sbjct: 231 RILGTYMPYSLEKREDGGFEARPTKE 256 >gb|AAG12703.1|AC021046_4 acid phosphatase, putative; 5376-6903 [Arabidopsis thaliana] gi|12325199|gb|AAG52550.1|AC013289_17 putative sterol desaturase; 75442-76969 [Arabidopsis thaliana] Length = 258 Score = 221 bits (562), Expect(2) = 2e-97 Identities = 105/150 (70%), Positives = 123/150 (82%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y + SL+ YRLHS+ +E+EKNL+SKS+V+KGVL+QQ VQAV AI+LF VTGS + Sbjct: 24 GIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLVQQVVQAVVAILLFTVTGSDAEAD 83 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 Q ++ VL Q AMIVLDTWQYFMHRYMH NKFLY+HIHSQHHRLIVPYAYGALYN Sbjct: 84 KAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKFLYKHIHSQHHRLIVPYAYGALYN 143 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGL+LDTIGGALSFL++GMSPR FF Sbjct: 144 HPVEGLLLDTIGGALSFLVSGMSPRTSIFF 173 Score = 164 bits (414), Expect(2) = 2e-97 Identities = 69/86 (80%), Positives = 81/86 (94%) Frame = -2 Query: 630 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWD 451 TSIFFFSFATIKTVDDHCGLWLPGNLFH+ F+NN+AYHD+HHQLYG+K+NFSQPFF+ WD Sbjct: 169 TSIFFFSFATIKTVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWD 228 Query: 450 KILGTYMPYTLEHREGGGYEARPKED 373 +ILGTYMPY+LE RE GG+EARP ++ Sbjct: 229 RILGTYMPYSLEKREDGGFEARPTKE 254 >ref|XP_006304118.1| hypothetical protein CARUB_v10010052mg [Capsella rubella] gi|482572829|gb|EOA37016.1| hypothetical protein CARUB_v10010052mg [Capsella rubella] Length = 259 Score = 223 bits (568), Expect(2) = 6e-97 Identities = 104/148 (70%), Positives = 123/148 (83%) Frame = -1 Query: 1060 YTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPASH 881 Y +GSLD+YRLHSR DEDEKNL++KSTV+KGVLLQQT+QA+ A++LF +TGS D Sbjct: 27 YICLGSLDKYRLHSRIDEDEKNLVTKSTVVKGVLLQQTLQAIIAVLLFKITGSDADAGMT 86 Query: 880 QYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYNHP 701 Q + VL Q AM+V+DTWQYF+HRYMH NKFLY+HIHSQHHRL+VPYAYGALYNHP Sbjct: 87 QQFSVLVLARQFIIAMLVIDTWQYFIHRYMHLNKFLYKHIHSQHHRLVVPYAYGALYNHP 146 Query: 700 IEGLILDTIGGALSFLLTGMSPRNFHFF 617 +EGL+LDTIGGALSFL +GMSPR FF Sbjct: 147 LEGLLLDTIGGALSFLFSGMSPRTAIFF 174 Score = 159 bits (403), Expect(2) = 6e-97 Identities = 68/83 (81%), Positives = 76/83 (91%) Frame = -2 Query: 630 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWD 451 T+IFFFSFATIKTVDDHCGLWLPGN FHIFF NN+AYHDVHHQLYGSK+NFSQPFF+ WD Sbjct: 170 TAIFFFSFATIKTVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGSKYNFSQPFFVMWD 229 Query: 450 KILGTYMPYTLEHREGGGYEARP 382 +ILGTY+PY+LE R GG+E RP Sbjct: 230 RILGTYLPYSLEKRANGGFETRP 252 >ref|XP_002888727.1| hypothetical protein ARALYDRAFT_476099 [Arabidopsis lyrata subsp. lyrata] gi|297334568|gb|EFH64986.1| hypothetical protein ARALYDRAFT_476099 [Arabidopsis lyrata subsp. lyrata] Length = 258 Score = 220 bits (561), Expect(2) = 1e-96 Identities = 103/150 (68%), Positives = 123/150 (82%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y + SL+ YRLHS+ +E+EKNL+SK++V+KGVL+QQ VQAV AI+LF VTGS + Sbjct: 24 GIYVALSSLESYRLHSKVEEEEKNLVSKASVVKGVLVQQVVQAVVAILLFTVTGSDAEAD 83 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 Q + VL Q AMI+LDTWQYFMHRYMHHNKFLY+HIHSQHHRLIVPYAYGALYN Sbjct: 84 KAQQFSLLVLARQFVTAMIILDTWQYFMHRYMHHNKFLYKHIHSQHHRLIVPYAYGALYN 143 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGL+LDT+GGALSFL++GMSPR FF Sbjct: 144 HPVEGLLLDTVGGALSFLVSGMSPRTSIFF 173 Score = 161 bits (408), Expect(2) = 1e-96 Identities = 68/86 (79%), Positives = 80/86 (93%) Frame = -2 Query: 630 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWD 451 TSIFFFSFATIKTVDDHCGLWLPGNLFH+ F+NN+AYHD+HHQ YG+K+NFSQPFF+ WD Sbjct: 169 TSIFFFSFATIKTVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQRYGTKYNFSQPFFVMWD 228 Query: 450 KILGTYMPYTLEHREGGGYEARPKED 373 +ILGTYMPY+LE RE GG+EARP ++ Sbjct: 229 RILGTYMPYSLEKREDGGFEARPTKE 254 >ref|NP_563944.1| sphingoid base hydroxylase 2 [Arabidopsis thaliana] gi|75261765|sp|Q9AST3.1|SBH2_ARATH RecName: Full=Sphinganine C(4)-monooxygenase 2; AltName: Full=Sphingoid C4-hydroxylase 2; AltName: Full=Sphingoid base hydroxylase 2 gi|13605750|gb|AAK32868.1|AF361856_1 At1g14290/F14L17_4 [Arabidopsis thaliana] gi|24797014|gb|AAN64519.1| At1g14290/F14L17_4 [Arabidopsis thaliana] gi|332191018|gb|AEE29139.1| sphingoid base hydroxylase 2 [Arabidopsis thaliana] Length = 259 Score = 223 bits (568), Expect(2) = 1e-96 Identities = 104/150 (69%), Positives = 125/150 (83%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y +GSLD+YRLHS+ DEDEKNL+SKS V+KGVLLQQT+QA+ ++ILF +TGS D A Sbjct: 25 GMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLLQQTLQAIISVILFKITGSDADAA 84 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 + Q + +L Q AM+V+DTWQYF+HRYMH NKFLY+HIHSQHHRLIVPY+YGALYN Sbjct: 85 TTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNKFLYKHIHSQHHRLIVPYSYGALYN 144 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGL+LDTIGGALSFL +GMSPR FF Sbjct: 145 HPLEGLLLDTIGGALSFLFSGMSPRTAIFF 174 Score = 158 bits (400), Expect(2) = 1e-96 Identities = 67/83 (80%), Positives = 76/83 (91%) Frame = -2 Query: 630 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWD 451 T+IFFFSFATIKTVDDHCGLWLPGN FHIFF NN+AYHDVHHQLYG+K+NFSQPFF+ WD Sbjct: 170 TAIFFFSFATIKTVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWD 229 Query: 450 KILGTYMPYTLEHREGGGYEARP 382 +ILGTY+PY+LE R GG+E RP Sbjct: 230 RILGTYLPYSLEKRANGGFETRP 252 >gb|AAF43928.1|AC012188_5 Contains similarity to acid phosphatase from Lupinus albus gb|AB023385 and contains a Sterol desaturase PF|01598 domain. EST gb|AI995340 comes from this gene [Arabidopsis thaliana] Length = 258 Score = 223 bits (568), Expect(2) = 1e-96 Identities = 104/150 (69%), Positives = 125/150 (83%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y +GSLD+YRLHS+ DEDEKNL+SKS V+KGVLLQQT+QA+ ++ILF +TGS D A Sbjct: 24 GMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLLQQTLQAIISVILFKITGSDADAA 83 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 + Q + +L Q AM+V+DTWQYF+HRYMH NKFLY+HIHSQHHRLIVPY+YGALYN Sbjct: 84 TTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNKFLYKHIHSQHHRLIVPYSYGALYN 143 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGL+LDTIGGALSFL +GMSPR FF Sbjct: 144 HPLEGLLLDTIGGALSFLFSGMSPRTAIFF 173 Score = 158 bits (400), Expect(2) = 1e-96 Identities = 67/83 (80%), Positives = 76/83 (91%) Frame = -2 Query: 630 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWD 451 T+IFFFSFATIKTVDDHCGLWLPGN FHIFF NN+AYHDVHHQLYG+K+NFSQPFF+ WD Sbjct: 169 TAIFFFSFATIKTVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWD 228 Query: 450 KILGTYMPYTLEHREGGGYEARP 382 +ILGTY+PY+LE R GG+E RP Sbjct: 229 RILGTYLPYSLEKRANGGFETRP 251 >ref|XP_006302713.1| hypothetical protein CARUB_v10020825mg [Capsella rubella] gi|482571423|gb|EOA35611.1| hypothetical protein CARUB_v10020825mg [Capsella rubella] Length = 259 Score = 217 bits (553), Expect(2) = 2e-96 Identities = 105/150 (70%), Positives = 120/150 (80%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y + SL+ YRLHS+ +E EKNL+SKS V+KGVLLQQ VQAV AI+LF VT S + Sbjct: 25 GIYVALSSLESYRLHSKAEEAEKNLVSKSAVVKGVLLQQVVQAVVAILLFTVTESDDEAD 84 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 Q + VL Q AMI+LDTWQYFMHRYMHHNKFLY+HIHSQHHRLIVPYAYGALYN Sbjct: 85 KAQQFSLLVLARQFVTAMIILDTWQYFMHRYMHHNKFLYKHIHSQHHRLIVPYAYGALYN 144 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGL+LDTIGGALSFL++GMSPR FF Sbjct: 145 HPVEGLLLDTIGGALSFLVSGMSPRTSIFF 174 Score = 164 bits (414), Expect(2) = 2e-96 Identities = 69/86 (80%), Positives = 81/86 (94%) Frame = -2 Query: 630 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWD 451 TSIFFFSFATIKTVDDHCGLWLPGNLFH+ F+NN+AYHD+HHQLYG+K+NFSQPFF+ WD Sbjct: 170 TSIFFFSFATIKTVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWD 229 Query: 450 KILGTYMPYTLEHREGGGYEARPKED 373 +ILGTYMPY+LE RE GG+EARP ++ Sbjct: 230 RILGTYMPYSLEKREDGGFEARPTKE 255 >ref|XP_006417030.1| hypothetical protein EUTSA_v10008529mg [Eutrema salsugineum] gi|557094801|gb|ESQ35383.1| hypothetical protein EUTSA_v10008529mg [Eutrema salsugineum] Length = 259 Score = 221 bits (563), Expect(2) = 2e-96 Identities = 103/150 (68%), Positives = 124/150 (82%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y +GSL+RYRLHS+ DEDEKNL++KS V+KGVLLQQ +QA+ ++ILF +TGS D A Sbjct: 25 GMYIFLGSLERYRLHSKIDEDEKNLVTKSAVVKGVLLQQILQAIISVILFKITGSDADAA 84 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 + Q + +L Q AM+V+DTWQYF+HRYMH NKFLY+HIHSQHHRLIVPYAYGALYN Sbjct: 85 TTQQLSLLLLARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYAYGALYN 144 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGL+LDTIGGALSFL +GMSPR FF Sbjct: 145 HPLEGLLLDTIGGALSFLFSGMSPRTAIFF 174 Score = 159 bits (403), Expect(2) = 2e-96 Identities = 68/83 (81%), Positives = 76/83 (91%) Frame = -2 Query: 630 TSIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWD 451 T+IFFFSFATIKTVDDHCGLWLPGN FHIFF NN+AYHDVHHQLYGSK+NFSQPFF+ WD Sbjct: 170 TAIFFFSFATIKTVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGSKYNFSQPFFVMWD 229 Query: 450 KILGTYMPYTLEHREGGGYEARP 382 +ILGTY+PY+LE R GG+E RP Sbjct: 230 RILGTYLPYSLEKRANGGFETRP 252 >ref|XP_004297306.1| PREDICTED: sphinganine C(4)-monooxygenase 2-like [Fragaria vesca subsp. vesca] Length = 257 Score = 219 bits (557), Expect(2) = 2e-96 Identities = 104/150 (69%), Positives = 125/150 (83%) Frame = -1 Query: 1066 GRYTMVGSLDRYRLHSRQDEDEKNLISKSTVIKGVLLQQTVQAVAAIILFAVTGSAVDPA 887 G Y ++ SL++YRLH+++DEDEKNL+SK TV+KGVLLQQT+QA+ AIILF VTG A Sbjct: 24 GIYVLLDSLEKYRLHTKKDEDEKNLVSKRTVVKGVLLQQTIQAIVAIILFTVTGDDGAAA 83 Query: 886 SHQYTTYFVLGLQIFGAMIVLDTWQYFMHRYMHHNKFLYRHIHSQHHRLIVPYAYGALYN 707 +++ L Q AM+VLDTWQYFMHRYMHHNKFLY+HIHSQHHRL+VPYA+GALYN Sbjct: 84 GAKHSL-IDLARQFVTAMLVLDTWQYFMHRYMHHNKFLYKHIHSQHHRLVVPYAFGALYN 142 Query: 706 HPIEGLILDTIGGALSFLLTGMSPRNFHFF 617 HP+EGL+LDTIGGALSFLL+GMSPR FF Sbjct: 143 HPVEGLLLDTIGGALSFLLSGMSPRASIFF 172 Score = 162 bits (409), Expect(2) = 2e-96 Identities = 68/85 (80%), Positives = 78/85 (91%) Frame = -2 Query: 627 SIFFFSFATIKTVDDHCGLWLPGNLFHIFFENNTAYHDVHHQLYGSKFNFSQPFFMWWDK 448 SIFFFSFATIKTVDDHCGLWLPGNLFH+ F NNTAYHDVHHQLYG+K+NFSQPFF+ WD+ Sbjct: 169 SIFFFSFATIKTVDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGNKYNFSQPFFVMWDR 228 Query: 447 ILGTYMPYTLEHREGGGYEARPKED 373 ILGTYMPY+L+ R GGG+E RP ++ Sbjct: 229 ILGTYMPYSLDKRTGGGFEVRPVKE 253