BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Achyranthes22_contig00015615
         (2173 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus ...   822   0.0  
gb|ADU15550.1| 4CL [Gossypium hirsutum]                               821   0.0  
gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum]          821   0.0  
gb|AFC89538.1| 4-coumarate: coenzyme A ligase 2 [Populus tomentosa]   818   0.0  
gb|EOY10077.1| 4-coumarate:CoA ligase 3 [Theobroma cacao]             818   0.0  
gb|ACZ06243.1| 4-coumarate:CoA ligase 2 [Gossypium hirsutum]          817   0.0  
ref|XP_002325815.1| 4-coumarate-CoA ligase family protein [Popul...   816   0.0  
gb|AAK58909.1| 4-coumarate:CoA ligase 4 [Populus trichocarpa x P...   811   0.0  
dbj|BAG82851.1| 4-coumarate:CoA ligase [Ipomoea batatas]              810   0.0  
gb|AAC24504.1| 4-coumarate:CoA ligase [Populus tremuloides]           809   0.0  
gb|AFG30056.1| 4-coumarate: CoA ligase [Malus hybrid cultivar]        808   0.0  
gb|ADE96997.1| p-coumarate:CoA-ligase 3 [Sorbus aucuparia]            807   0.0  
ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis ...   807   0.0  
gb|AEX32786.1| coumaroyl-CoA ligase [Vitis vinifera]                  806   0.0  
ref|XP_006443197.1| hypothetical protein CICLE_v10019532mg [Citr...   803   0.0  
gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]                  801   0.0  
gb|AGW16013.1| 4-coumarate:coenzyme A ligase 1 [Pueraria montana...   801   0.0  
sp|P31687.2|4CL2_SOYBN RecName: Full=4-coumarate--CoA ligase 2; ...   800   0.0  
ref|XP_004307864.1| PREDICTED: LOW QUALITY PROTEIN: 4-coumarate-...   796   0.0  
gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]        795   0.0  

>ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus communis]
            gi|223540792|gb|EEF42352.1| AMP dependent CoA ligase,
            putative [Ricinus communis]
          Length = 573

 Score =  822 bits (2123), Expect = 0.0
 Identities = 414/561 (73%), Positives = 477/561 (85%), Gaps = 7/561 (1%)
 Frame = -1

Query: 2092 QPIDSEISSLKSPQK--PENLKTE-HIFKSKLPDIPITNHLPLHSYLFEKLPQVSEKPCL 1922
            +P   E+SS  S QK  P +  +E HIF+SKLPDIPI+NHLPLH+Y FE L ++S+KPCL
Sbjct: 8    EPPKQELSSSSSSQKQNPSSSSSETHIFRSKLPDIPISNHLPLHTYCFENLFKLSDKPCL 67

Query: 1921 ISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMIGAVT 1742
            ISG+TGK YSFAETHL+ +KT AGLS LG+KKGDVIMILL NCPEFVF+F G SMIGAV 
Sbjct: 68   ISGSTGKTYSFAETHLISQKTAAGLSSLGIKKGDVIMILLQNCPEFVFSFMGASMIGAVA 127

Query: 1741 TTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESE---EFQINQDFRVITIDNPPEN 1571
            TTANPFYT+ EIFKQ+  S  K+I+TQS +VDKL++ +   + ++ +DF VITID PPEN
Sbjct: 128  TTANPFYTSNEIFKQLNSSRPKLIITQSLYVDKLRDPDHKIQPKLGEDFTVITIDEPPEN 187

Query: 1570 CLHFSILSEANESDLPET-KINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQVDGE 1394
            CLHF++LSEA+E+++PE    NPDDPV LPFSSGTTGLPKGVILTHKSL TSVAQQVDGE
Sbjct: 188  CLHFTVLSEASETEIPEVINANPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGE 247

Query: 1393 NPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHRXXX 1214
            NPN+ L EDDV+LCVLPLFHI+SLNSVL+ +LR G A+LLM KF+I +LLELIQKH+   
Sbjct: 248  NPNLYLKEDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFQIGALLELIQKHKVSV 307

Query: 1213 XXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYGMTE 1034
                       AKNPMV +FDLSSIR+V+SGAAPLGKELEDALR R+PQA+LGQGYGMTE
Sbjct: 308  AAVVPPLVLALAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTE 367

Query: 1033 AGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQIMK 854
            AGPVLSMC  FAK+P  TK GSCG VVRNAE+K++DPETG SLGYN+ GEICIRG QIMK
Sbjct: 368  AGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGPQIMK 427

Query: 853  EYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEALLV 674
             YLNDP ATA TID+EGWLHTGDIGYVD+DDE+FIVDRVKEIIKFKGFQVPPAELEALL+
Sbjct: 428  GYLNDPEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAELEALLL 487

Query: 673  NHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTVYFI 494
            NHPSIADAAVVPQKDE AGEVPVAFVV+S NG +L+EE VK+YIAKQVVFYKKLH VYFI
Sbjct: 488  NHPSIADAAVVPQKDEVAGEVPVAFVVRS-NGFELSEEDVKEYIAKQVVFYKKLHKVYFI 546

Query: 493  HAIPKSASGKILRKELRARLA 431
            HAIPKS SGKILRK+L+A+LA
Sbjct: 547  HAIPKSPSGKILRKDLKAKLA 567


>gb|ADU15550.1| 4CL [Gossypium hirsutum]
          Length = 574

 Score =  821 bits (2121), Expect = 0.0
 Identities = 410/572 (71%), Positives = 483/572 (84%), Gaps = 12/572 (2%)
 Frame = -1

Query: 2110 MAAVQSQPIDSEISSLKSPQKPENLKTE----HIFKSKLPDIPITNHLPLHSYLFEKLPQ 1943
            M ++   P++ E      P KP+ L  E    HIF+SKLPDIPI+NHLPLH+Y FE LP 
Sbjct: 1    MISIADHPVELE------PPKPDQLSPETPADHIFRSKLPDIPISNHLPLHTYCFENLPS 54

Query: 1942 VSEKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGT 1763
            +S+KPCLISG++GKIYSF+ETHL+ +KT AGLSKLG++KGD IMILL NC EFVF F G 
Sbjct: 55   LSDKPCLISGSSGKIYSFSETHLIAQKTAAGLSKLGIQKGDAIMILLHNCAEFVFAFMGA 114

Query: 1762 SMIGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKES-------EEF-QINQD 1607
            SMIGAV+TTANPFYT+ EIFKQ K + AK+I+TQS++VDKLK++       E F +I +D
Sbjct: 115  SMIGAVSTTANPFYTSKEIFKQSKAARAKLIITQSQYVDKLKDTNNNNDGVENFPKIGED 174

Query: 1606 FRVITIDNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSL 1427
            F+VITID+PPENCL FS+LSEANE+++P+  I+PDDPV LPFSSGTTGLPKGV+LTHKSL
Sbjct: 175  FKVITIDDPPENCLDFSVLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSL 234

Query: 1426 STSVAQQVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSL 1247
             TSVAQQVDGENPN+ LT +DV+LCVLPLFHI+SLNSVL+ +LR G A+LLM KFEI +L
Sbjct: 235  ITSVAQQVDGENPNLYLTHEDVVLCVLPLFHIYSLNSVLLWSLRAGAAVLLMQKFEIGTL 294

Query: 1246 LELIQKHRXXXXXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQ 1067
            LELIQ+H+              AKNP+V +FDLSSIR+V+SGAAPLGKELEDALR R+PQ
Sbjct: 295  LELIQRHKVSVAAVVPPLVVLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEDALRGRVPQ 354

Query: 1066 AVLGQGYGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAG 887
            AVLGQGYGMTEAGPVLSMC  FAK+P  TK GSCG VVRNAE+K++DPETG SLGYN+ G
Sbjct: 355  AVLGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPG 414

Query: 886  EICIRGAQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQ 707
            EICIRG+QIMK YLND  ATAATID+EGWLHTGDIGYVD DDE+FIVDRVKEIIKFKGFQ
Sbjct: 415  EICIRGSQIMKGYLNDGVATAATIDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQ 474

Query: 706  VPPAELEALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVV 527
            VPPAELE+LL++HPSIADAAVVPQKDE AGEVPVAFVV+S NG +LTEEAVK++IAKQVV
Sbjct: 475  VPPAELESLLISHPSIADAAVVPQKDEVAGEVPVAFVVRS-NGFELTEEAVKEFIAKQVV 533

Query: 526  FYKKLHTVYFIHAIPKSASGKILRKELRARLA 431
            +YK+LH V+F+HAIPKS SGKILRK+LRA+LA
Sbjct: 534  YYKRLHKVHFVHAIPKSPSGKILRKDLRAKLA 565


>gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum]
          Length = 573

 Score =  821 bits (2120), Expect = 0.0
 Identities = 408/567 (71%), Positives = 480/567 (84%), Gaps = 7/567 (1%)
 Frame = -1

Query: 2110 MAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVSEK 1931
            M ++   P+  E+ S K  Q       +HIF+SKLPDIPI+NHLPLH+Y FE LP +S+K
Sbjct: 1    MISIADHPV--ELESPKPDQLSPETPADHIFRSKLPDIPISNHLPLHTYCFENLPSLSDK 58

Query: 1930 PCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMIG 1751
            PCLISG++GKIYSF+ETHL+ +KT AGLS LG++KGD IMILL NC EFVF F G SMIG
Sbjct: 59   PCLISGSSGKIYSFSETHLIAQKTAAGLSNLGIQKGDAIMILLHNCAEFVFAFMGASMIG 118

Query: 1750 AVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKES------EEF-QINQDFRVIT 1592
            AV+TTANPFYT+ EIFKQ K + AK+I+TQS++VDKLK++      E F +I +DF+VIT
Sbjct: 119  AVSTTANPFYTSKEIFKQFKAARAKLIITQSQYVDKLKDTNNNDGDENFPKIGEDFKVIT 178

Query: 1591 IDNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVA 1412
            ID+PPENCL FS+LSEANE+++P+  I+PDDPV LPFSSGTTGLPKGV+LTHKSL TSVA
Sbjct: 179  IDDPPENCLDFSMLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITSVA 238

Query: 1411 QQVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQ 1232
            QQVDGENPN+ LT DDV+LCVLPLFHI+SLNSVL+ +LR G A+LLM KFEI +LLELIQ
Sbjct: 239  QQVDGENPNLYLTHDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELIQ 298

Query: 1231 KHRXXXXXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQ 1052
            +H+              AKNP+V +FDLSSIR+V+SGAAPLGKELEDALR R+PQAVLGQ
Sbjct: 299  RHKVSVAAVVPPLVVLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEDALRGRVPQAVLGQ 358

Query: 1051 GYGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIR 872
            GYGMTEAGPVLSMC  FAK+P  TK GSCG VVRNAE+K++DPETG SLGYN+ GEICIR
Sbjct: 359  GYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIR 418

Query: 871  GAQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAE 692
            G+QIMK YLND  ATAATID+EGWLHTGDIGYVD DDE+FIVDRVKEIIKFKGFQVPPAE
Sbjct: 419  GSQIMKGYLNDGVATAATIDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQVPPAE 478

Query: 691  LEALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKL 512
            LE+LL++HPSIADAAVVPQKDE AGEVPVAFVV+S NG +LTEEAVK++IAKQVV+YK+L
Sbjct: 479  LESLLISHPSIADAAVVPQKDEVAGEVPVAFVVRS-NGFELTEEAVKEFIAKQVVYYKRL 537

Query: 511  HTVYFIHAIPKSASGKILRKELRARLA 431
            H V+F+HAIPKS SGKILRK+LRA+LA
Sbjct: 538  HKVHFVHAIPKSPSGKILRKDLRAKLA 564


>gb|AFC89538.1| 4-coumarate: coenzyme A ligase 2 [Populus tomentosa]
          Length = 569

 Score =  818 bits (2113), Expect = 0.0
 Identities = 415/566 (73%), Positives = 474/566 (83%), Gaps = 4/566 (0%)
 Frame = -1

Query: 2116 MSMAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVS 1937
            MS+A V+  P   E+S  ++   P + +T+HIF+SKLPDI I+NHLPLH+Y FE L   S
Sbjct: 1    MSVATVE--PPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENLSDFS 58

Query: 1936 EKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSM 1757
            ++PCLISG+TGK YSFAETHL+ RK  AGLS LG+KKGDVIM LL NCPEFVF+F G SM
Sbjct: 59   DRPCLISGSTGKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMGASM 118

Query: 1756 IGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQINQ----DFRVITI 1589
            IGAVTTTANPFYT  EIFKQ   S AK+I+TQS++V+KL +S+  + NQ    DF VITI
Sbjct: 119  IGAVTTTANPFYTQSEIFKQFSASRAKLIITQSQYVNKLGDSDCHENNQKPGEDFIVITI 178

Query: 1588 DNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQ 1409
            D+PPENC HF++L EANES++P   I PDDPV LPFSSGTTGLPKGVILTHKSL TSVAQ
Sbjct: 179  DDPPENCRHFNVLVEANESEMPTISILPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQ 238

Query: 1408 QVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQK 1229
            QVDGE PN+ L +DDV+LCVLPLFHIFSLNSVL+ +LR G A+LLM KFEI SLLELIQK
Sbjct: 239  QVDGEIPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQK 298

Query: 1228 HRXXXXXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQG 1049
            H               AKNP+V  FDLSSIR+V+SGAAPLGKELEDALR R+PQA+LGQG
Sbjct: 299  HNVSVAAVVPPLVLALAKNPLVANFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQG 358

Query: 1048 YGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRG 869
            YGMTEAGPVLSMC AF+K+P  TK GSCG VVRNAE+K++DPETG SLGYN+ GEICIRG
Sbjct: 359  YGMTEAGPVLSMCLAFSKQPFPTKSGSCGTVVRNAELKVIDPETGGSLGYNQPGEICIRG 418

Query: 868  AQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAEL 689
            +QIMK YLND  ATA TID+EGWLHTGDIGYVD+DDE+FIVDRVKEIIKFKGFQVPPAEL
Sbjct: 419  SQIMKGYLNDAEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAEL 478

Query: 688  EALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLH 509
            EALLVNHPSIADAAVVPQKDE AGEVPVAFVV+S + LDL+EEAVKDYIAKQVVFYKKLH
Sbjct: 479  EALLVNHPSIADAAVVPQKDEVAGEVPVAFVVRS-DDLDLSEEAVKDYIAKQVVFYKKLH 537

Query: 508  TVYFIHAIPKSASGKILRKELRARLA 431
             V+F+H+IPKSASGKILRK+LRA+LA
Sbjct: 538  KVFFVHSIPKSASGKILRKDLRAKLA 563


>gb|EOY10077.1| 4-coumarate:CoA ligase 3 [Theobroma cacao]
          Length = 575

 Score =  818 bits (2112), Expect = 0.0
 Identities = 409/561 (72%), Positives = 480/561 (85%), Gaps = 7/561 (1%)
 Frame = -1

Query: 2092 QPIDSEISSLKSPQ--KPENLKT-EHIFKSKLPDIPITNHLPLHSYLFEKLPQVSEKPCL 1922
            +P   E+S   SP   KP +  T +HIF+SKLPDIPI+NHLPLH+Y FE L    +KPCL
Sbjct: 9    EPQKPELSPSLSPPATKPSSETTGDHIFRSKLPDIPISNHLPLHTYCFENLSSFPDKPCL 68

Query: 1921 ISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMIGAVT 1742
            ISG++GK YSF+ETHL+ +KT AGLS LG++KGDVIMILL NC EFVF+F G SMIGAV+
Sbjct: 69   ISGSSGKTYSFSETHLIAQKTAAGLSNLGIRKGDVIMILLQNCAEFVFSFMGASMIGAVS 128

Query: 1741 TTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKES---EEF-QINQDFRVITIDNPPE 1574
            TTANPFYT+ EIFKQ K++ AK+IVTQS++VDKLK++   E F ++ +DF+VITID+PPE
Sbjct: 129  TTANPFYTSTEIFKQYKVAQAKLIVTQSQYVDKLKDTSNNENFPKMGEDFKVITIDDPPE 188

Query: 1573 NCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQVDGE 1394
            NCLHF++LSEANE+++P+  I+PDDPV LPFSSGTTGLPKGV+LTHKSL TSVAQQVDGE
Sbjct: 189  NCLHFTVLSEANENEIPQVSIDPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGE 248

Query: 1393 NPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHRXXX 1214
            NPN+ L  DDV+LCVLPLFHI+SLNSVL+ ALR G A+LLM KFEI +LLELIQKH+   
Sbjct: 249  NPNLYLKHDDVVLCVLPLFHIYSLNSVLLCALRAGAAVLLMQKFEIGALLELIQKHKVSV 308

Query: 1213 XXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYGMTE 1034
                       AKNPMV ++DLSSIR+V+SGAAPLGKELE+ALR R+PQAVLGQGYGMTE
Sbjct: 309  AAVVPPLVLALAKNPMVAQYDLSSIRVVLSGAAPLGKELEEALRGRVPQAVLGQGYGMTE 368

Query: 1033 AGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQIMK 854
            AGPVLSMC  FAK+P  TK GSCG VVRNAE+K++DPETG SLGYN+ GEICIRG+QIMK
Sbjct: 369  AGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGSQIMK 428

Query: 853  EYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEALLV 674
             YLND +ATA TID+EGWLHTGDIGYVD+ DE+FIVDRVKEIIKFKGFQVPPAELE+LLV
Sbjct: 429  GYLNDTSATATTIDVEGWLHTGDIGYVDDGDEIFIVDRVKEIIKFKGFQVPPAELESLLV 488

Query: 673  NHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTVYFI 494
            +HPSIADAAVVPQKDE AGEVPVAFVV+S NG +LTEEAVK++IAKQVVFYKKLH V+F+
Sbjct: 489  SHPSIADAAVVPQKDEVAGEVPVAFVVRS-NGFELTEEAVKEFIAKQVVFYKKLHKVHFV 547

Query: 493  HAIPKSASGKILRKELRARLA 431
            HAIPKS SGKILRK+LRA+LA
Sbjct: 548  HAIPKSPSGKILRKDLRAKLA 568


>gb|ACZ06243.1| 4-coumarate:CoA ligase 2 [Gossypium hirsutum]
          Length = 574

 Score =  817 bits (2111), Expect = 0.0
 Identities = 405/568 (71%), Positives = 480/568 (84%), Gaps = 8/568 (1%)
 Frame = -1

Query: 2110 MAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVSEK 1931
            M ++   P+  E+ S K  Q       +HIF+SKLPDIPI+NHLPLH+Y FE LP +S+K
Sbjct: 1    MISIADHPV--ELESPKPDQLSPETPADHIFRSKLPDIPISNHLPLHTYCFENLPSLSDK 58

Query: 1930 PCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMIG 1751
            PCLISG++GKIYSF+ETHL+ +KT AGLSKLG++KGD IMILL NC EFVF F G SMIG
Sbjct: 59   PCLISGSSGKIYSFSETHLIAQKTAAGLSKLGIQKGDAIMILLHNCAEFVFAFMGASMIG 118

Query: 1750 AVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKES-------EEF-QINQDFRVI 1595
            AV+TTANPFYT+ EIFKQ K + AK+I+TQS++VDKLK++       E F +I +DF+VI
Sbjct: 119  AVSTTANPFYTSKEIFKQFKAARAKLIITQSQYVDKLKDTNNNNDGVENFPKIGEDFKVI 178

Query: 1594 TIDNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSV 1415
            TID+PPENCL FS+LSEANE+++P+  I+PDDPV LPFSSGTTGLPKGV+LTHKSL TSV
Sbjct: 179  TIDDPPENCLDFSVLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITSV 238

Query: 1414 AQQVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELI 1235
            AQQVDGENPN+ LT +DV+LCVLPLFHI+SLNSVL+ +LR G A+LLM KFEI +LLELI
Sbjct: 239  AQQVDGENPNLYLTHEDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELI 298

Query: 1234 QKHRXXXXXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLG 1055
            Q+H+              AKNP+V +FDLSSIR+V+SGAAPLGKELE+ALR R+PQAVLG
Sbjct: 299  QRHKVSVAAVVPPLVLLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEEALRGRVPQAVLG 358

Query: 1054 QGYGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICI 875
            QGYGMTEAGPVLSMC  FAK+P  TK GSCG VVRNAE+K++DPETG SLGYN+ GEICI
Sbjct: 359  QGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICI 418

Query: 874  RGAQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPA 695
            RG+QIMK YLND  ATAAT D+EGWLHTGDIGYVD DDE+FIVDRVKEIIKFKGFQVPPA
Sbjct: 419  RGSQIMKGYLNDSVATAATTDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQVPPA 478

Query: 694  ELEALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKK 515
            ELE+LL++HPSIADAAVVPQKDE AGEVPVAFVV+S NG +LTEEAVK++IA+QVV+YK+
Sbjct: 479  ELESLLISHPSIADAAVVPQKDEVAGEVPVAFVVRS-NGFELTEEAVKEFIARQVVYYKR 537

Query: 514  LHTVYFIHAIPKSASGKILRKELRARLA 431
            LH V+F+HAIPKS SGKILRK+LRA+LA
Sbjct: 538  LHKVHFVHAIPKSPSGKILRKDLRAKLA 565


>ref|XP_002325815.1| 4-coumarate-CoA ligase family protein [Populus trichocarpa]
            gi|118487236|gb|ABK95446.1| unknown [Populus trichocarpa]
            gi|222862690|gb|EEF00197.1| 4-coumarate-CoA ligase family
            protein [Populus trichocarpa]
          Length = 570

 Score =  816 bits (2109), Expect = 0.0
 Identities = 414/566 (73%), Positives = 473/566 (83%), Gaps = 4/566 (0%)
 Frame = -1

Query: 2116 MSMAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVS 1937
            MS+A V+  P   E+S  ++   P + +T+HIF+SKLPDI I+NHLPLH+Y FE L   S
Sbjct: 2    MSVATVE--PPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENLSDFS 59

Query: 1936 EKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSM 1757
            ++PCLISG+TGK YSFAETHL+ RK  AGLS LG+KKGDVIM LL NCPEFVF+F G SM
Sbjct: 60   DRPCLISGSTGKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMGASM 119

Query: 1756 IGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQINQ----DFRVITI 1589
            IGAVTTT NPFYT  EIFKQ   S AK+I+TQS+ V+KL++S+  + NQ    DF VITI
Sbjct: 120  IGAVTTTVNPFYTPGEIFKQFSASRAKLIITQSQHVNKLRDSDCHENNQKPEEDFIVITI 179

Query: 1588 DNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQ 1409
            D+PPENCLHF++L EANES++P   I+PDDPV LPFSSGTTGLPKGVILTHKSL TSVAQ
Sbjct: 180  DDPPENCLHFNVLVEANESEMPTVSIHPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQ 239

Query: 1408 QVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQK 1229
            QVDGE PN+ L +DDV+LCVLPLFHIFSLNSVL+ +LR G A+LLM KFEI SLLELIQK
Sbjct: 240  QVDGEIPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQK 299

Query: 1228 HRXXXXXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQG 1049
            H               AKNPMV  FDLSSIR+V+SGAAPLGKELE+ALR R+PQA+LGQG
Sbjct: 300  HNVSVAAVVPPLVLALAKNPMVANFDLSSIRVVLSGAAPLGKELEEALRSRVPQAILGQG 359

Query: 1048 YGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRG 869
            YGMTEAGPVLSMC AF+K+PL TK GSCG VVRNAE+K++DPETG SLG N+ GEICIRG
Sbjct: 360  YGMTEAGPVLSMCLAFSKQPLPTKSGSCGTVVRNAELKVIDPETGSSLGRNQPGEICIRG 419

Query: 868  AQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAEL 689
            +QIMK YLND  ATA  ID+EGWLHTGDIGYVD+DDE+FIVDRVKEIIKFKGFQVPPAEL
Sbjct: 420  SQIMKGYLNDAEATANIIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAEL 479

Query: 688  EALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLH 509
            EALLVNHPSIADAAVVPQKDE AGEVPVAFVV+S N LDL EEAVKDYIAKQVVFYKKLH
Sbjct: 480  EALLVNHPSIADAAVVPQKDEVAGEVPVAFVVRS-NDLDLNEEAVKDYIAKQVVFYKKLH 538

Query: 508  TVYFIHAIPKSASGKILRKELRARLA 431
             V+F+H+IPKSA+GKILRK+LRA+LA
Sbjct: 539  KVFFVHSIPKSAAGKILRKDLRAKLA 564


>gb|AAK58909.1| 4-coumarate:CoA ligase 4 [Populus trichocarpa x Populus deltoides]
          Length = 579

 Score =  811 bits (2094), Expect = 0.0
 Identities = 415/575 (72%), Positives = 473/575 (82%), Gaps = 13/575 (2%)
 Frame = -1

Query: 2116 MSMAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVS 1937
            MS+A V+  P   E+S  ++   P + +T+HIF+SKLPDI I+NHLPLH+Y FE L   S
Sbjct: 2    MSVATVE--PPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENLSDFS 59

Query: 1936 EKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSM 1757
            ++PCLISG+TGK YSFAETHL+ RK  AGLS LG+KKGDVIM LL NCPEFVF+F G SM
Sbjct: 60   DRPCLISGSTGKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMGASM 119

Query: 1756 IGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQINQ----DFRVITI 1589
            IGAVTTT NPFYT  EIFKQ   S AK+I+TQS+ V+KL++S+  + NQ    DF VITI
Sbjct: 120  IGAVTTTVNPFYTPGEIFKQFSASRAKLIITQSQHVNKLRDSDYHENNQKPEEDFIVITI 179

Query: 1588 DNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQ 1409
            D+PPENCLHF++L EANES++P   I+PDDPV LPFSSGTTGLPKGVILTHKSL TSVAQ
Sbjct: 180  DDPPENCLHFNVLVEANESEMPTVSIHPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQ 239

Query: 1408 QVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQK 1229
            QVDGE PN+ L +DDV+LCVLPLFHIFSLNSVL+ +LR G A+LLM KFEI SLLELIQK
Sbjct: 240  QVDGEIPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQK 299

Query: 1228 HRXXXXXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQG 1049
            H               AKNPMV  FDLSSIR+V+SGAAPLGKELE+ALR R+PQA+LGQG
Sbjct: 300  HNVSVAAVVPPLVLALAKNPMVANFDLSSIRVVLSGAAPLGKELEEALRSRVPQAILGQG 359

Query: 1048 YGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRG 869
            YGMTEAGPVLSMC AF+K+PL TK GSCG VVRNAE+K++DPETG SLG N+ GEICIRG
Sbjct: 360  YGMTEAGPVLSMCLAFSKQPLPTKSGSCGTVVRNAELKVIDPETGSSLGRNQPGEICIRG 419

Query: 868  AQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAEL 689
            +QIMK YLND  ATA  ID+EGWLHTGDIGYVD+DDE+FIVDRVKEIIKFKGFQVPPAEL
Sbjct: 420  SQIMKGYLNDAEATANIIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAEL 479

Query: 688  EALLVNHPSIADAAVVP---------QKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAK 536
            EALLVNHPSIADAAVVP         QKDE AGEVPVAFVV+S N LDL EEAVKDYIAK
Sbjct: 480  EALLVNHPSIADAAVVPRDNLYGNNRQKDEVAGEVPVAFVVRS-NDLDLNEEAVKDYIAK 538

Query: 535  QVVFYKKLHTVYFIHAIPKSASGKILRKELRARLA 431
            QVVFYKKLH V+F+H+IPKSASGKILRK+LRA+LA
Sbjct: 539  QVVFYKKLHKVFFVHSIPKSASGKILRKDLRAKLA 573


>dbj|BAG82851.1| 4-coumarate:CoA ligase [Ipomoea batatas]
          Length = 569

 Score =  810 bits (2091), Expect = 0.0
 Identities = 405/562 (72%), Positives = 474/562 (84%), Gaps = 3/562 (0%)
 Frame = -1

Query: 2110 MAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVSEK 1931
            M +V++Q  + E++     Q   +    HIF+S+LPDIPI N +PLH+Y F+ L +  ++
Sbjct: 1    MLSVETQKPELEVADSGKTQAQSS--QIHIFRSRLPDIPIPNQIPLHTYCFQNLAEYRDR 58

Query: 1930 PCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMIG 1751
             CLI G+TGK YSFAETHL+CRK  +GL+KLGVKKGDVIM L  NCPEFVF+F G SMIG
Sbjct: 59   TCLIVGSTGKTYSFAETHLICRKVASGLAKLGVKKGDVIMTLFQNCPEFVFSFMGASMIG 118

Query: 1750 AVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKES-EEF-QINQDFRVITIDNPP 1577
            AVTTTANPFYT  EIFKQ+  S AK+++TQS +VDKLK+S EE+ +I +DF V+TID+PP
Sbjct: 119  AVTTTANPFYTKAEIFKQMSASKAKVVITQSVYVDKLKDSGEEYPKIGEDFSVVTIDDPP 178

Query: 1576 ENCLHFSILSEANESDLPET-KINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQVD 1400
            ENCLHFS+LSEA+E D+P T +I PDD V LPFSSGTTGLPKGV+LTHKSL TSVAQQVD
Sbjct: 179  ENCLHFSVLSEADEEDMPATVEIAPDDAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVD 238

Query: 1399 GENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHRX 1220
            GENPN+ L EDDV+LCVLPLFHIFSLNSVL+ +LR G A+LLM KFEI SLLELI+KHR 
Sbjct: 239  GENPNLYLKEDDVVLCVLPLFHIFSLNSVLLCSLRAGAAVLLMQKFEIKSLLELIKKHRV 298

Query: 1219 XXXXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYGM 1040
                         AKNP+V+ +DLSSIR+V+SGAAPLGKELE+AL +R+PQA+ GQGYGM
Sbjct: 299  SVAAVVPPLVLALAKNPIVDSYDLSSIRVVLSGAAPLGKELEEALHQRVPQAIFGQGYGM 358

Query: 1039 TEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQI 860
            TEAGPVLSMCPAFAK+ L  K GSCG VVRNAE+ +VDPETG SLG N+ GEICIRG+QI
Sbjct: 359  TEAGPVLSMCPAFAKQALPAKSGSCGSVVRNAELMVVDPETGCSLGRNQPGEICIRGSQI 418

Query: 859  MKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEAL 680
            MKEYLNDPAATA TID++GWLHTGDIGYVD+DDEVFIVDRVKE+IKFKGFQVPPAELEAL
Sbjct: 419  MKEYLNDPAATARTIDVDGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEAL 478

Query: 679  LVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTVY 500
            L++HP IADAAVVPQKD+AAGEVPVAFVV+S++G DLTEEAVK++IAKQVVFYKKLH VY
Sbjct: 479  LLSHPMIADAAVVPQKDDAAGEVPVAFVVRSSDGFDLTEEAVKEFIAKQVVFYKKLHKVY 538

Query: 499  FIHAIPKSASGKILRKELRARL 434
            FIHAIPKSASGKILRKELR +L
Sbjct: 539  FIHAIPKSASGKILRKELREKL 560


>gb|AAC24504.1| 4-coumarate:CoA ligase [Populus tremuloides]
          Length = 570

 Score =  809 bits (2090), Expect = 0.0
 Identities = 411/566 (72%), Positives = 472/566 (83%), Gaps = 4/566 (0%)
 Frame = -1

Query: 2116 MSMAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVS 1937
            MS+A V+  P   E+S  ++   P + +T+HIF+SKLPDI I+N LPLH+Y FE L   S
Sbjct: 2    MSVATVE--PPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNDLPLHAYCFENLSDFS 59

Query: 1936 EKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSM 1757
            ++PCLISG+TGK YSFAETHL+ RK  AGLS LG+KKGDVIM LL NCPEFVF+F G SM
Sbjct: 60   DRPCLISGSTGKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFIGASM 119

Query: 1756 IGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQINQ----DFRVITI 1589
            IGAV TTANPFYT  EIFKQ   S AK+I+TQS++V+KL +S+  + NQ    DF VITI
Sbjct: 120  IGAVITTANPFYTQSEIFKQFSASRAKLIITQSQYVNKLGDSDCHENNQKPGEDFIVITI 179

Query: 1588 DNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQ 1409
            D+PPENCLHF++L EA+ES++P   I PDDPV LPFSSGTTGLPKGVILTHKSL TSVAQ
Sbjct: 180  DDPPENCLHFNVLVEASESEMPTVSILPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQ 239

Query: 1408 QVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQK 1229
            QVDGE PN+ L +DDV+LCVLPLFHIFSLNSVL+ +LR G A+LLM KFEI SLLELIQK
Sbjct: 240  QVDGEIPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQK 299

Query: 1228 HRXXXXXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQG 1049
            H               AKNP+   FDLSSIR+V+SGAAPLGKELEDALR R+PQA+LGQG
Sbjct: 300  HNVSVAAVVPPLVLALAKNPLEANFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQG 359

Query: 1048 YGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRG 869
            YGMTEAGPVLSMC AF+K+P  TK GSCG VVRNAE+K++DPETG SLGYN+ GEICIRG
Sbjct: 360  YGMTEAGPVLSMCLAFSKQPFPTKSGSCGTVVRNAELKVIDPETGRSLGYNQPGEICIRG 419

Query: 868  AQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAEL 689
            +QIMK YLND  ATA TID+EGWLHTGDIGYVD+DDE+FIVDRVKEIIKFKGFQVPPAEL
Sbjct: 420  SQIMKGYLNDAEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAEL 479

Query: 688  EALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLH 509
            EALLVNHPSIADAAVVPQKDE AGEVPVAFVV+S + LDL+EEAVK+YIAKQVVFYKKLH
Sbjct: 480  EALLVNHPSIADAAVVPQKDEVAGEVPVAFVVRS-DDLDLSEEAVKEYIAKQVVFYKKLH 538

Query: 508  TVYFIHAIPKSASGKILRKELRARLA 431
             V+F+H+IPKSASGKILRK+LRA+LA
Sbjct: 539  KVFFVHSIPKSASGKILRKDLRAKLA 564


>gb|AFG30056.1| 4-coumarate: CoA ligase [Malus hybrid cultivar]
          Length = 605

 Score =  808 bits (2088), Expect = 0.0
 Identities = 404/588 (68%), Positives = 483/588 (82%), Gaps = 10/588 (1%)
 Frame = -1

Query: 2164 LSPSIIFFTTHHFQTNMSMAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITN 1985
            L P +I  T+    T +   A  +  IDS  ++             H+F+SKLPDIPI N
Sbjct: 21   LMPPLINSTSQQNLTQLQPPACTNNIIDSTTATAT---------INHVFRSKLPDIPIPN 71

Query: 1984 HLPLHSYLFEKLPQVSEKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMIL 1805
            HLPLH+Y F+ LP+ S+KPCLI G+TGK YSF+ETHL+ +KTGAGLS LG++KGDVIMIL
Sbjct: 72   HLPLHTYCFQNLPEFSDKPCLIVGSTGKSYSFSETHLISQKTGAGLSNLGIQKGDVIMIL 131

Query: 1804 LPNCPEFVFTFFGTSMIGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEE 1625
            L NC EFVF F G S+IGAVTTTANPFYT+ EIFKQVK + AK+I+TQS++VDKL+E   
Sbjct: 132  LQNCAEFVFAFMGASLIGAVTTTANPFYTSAEIFKQVKAANAKLIITQSQYVDKLREHPS 191

Query: 1624 F----------QINQDFRVITIDNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSS 1475
                       ++ +DF+V+TID+PPENCLHFS+ SEANE +LP+  I+ +DPV LPFSS
Sbjct: 192  SADGADQNNYPKLGEDFKVVTIDDPPENCLHFSVFSEANEKELPDVVIDAEDPVALPFSS 251

Query: 1474 GTTGLPKGVILTHKSLSTSVAQQVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALR 1295
            GTTGLPKGVILTHKSL TSVAQQVDGENPN+ L EDDV+LCVLPLFHIFSLNSVL+ +LR
Sbjct: 252  GTTGLPKGVILTHKSLVTSVAQQVDGENPNLYLKEDDVVLCVLPLFHIFSLNSVLLCSLR 311

Query: 1294 TGCAILLMPKFEINSLLELIQKHRXXXXXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAA 1115
             G  +LLM KFEI +LLELIQ++R              AKNPMV +FDLSSIR+V+SGAA
Sbjct: 312  AGAGVLLMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKNPMVAEFDLSSIRVVLSGAA 371

Query: 1114 PLGKELEDALRERIPQAVLGQGYGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMK 935
            PLGKELE+AL+ R+PQA+LGQGYGMTEAGPVLSMC AFAKEP+ TK GSCG VVRNAE+K
Sbjct: 372  PLGKELEEALKSRVPQALLGQGYGMTEAGPVLSMCMAFAKEPMPTKSGSCGTVVRNAELK 431

Query: 934  IVDPETGVSLGYNEAGEICIRGAQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEV 755
            ++D ETG+SLGYN++GEICIRG+QIMK YLND AATA T+D EGWLHTGD+GYVD+D+E+
Sbjct: 432  VLDLETGLSLGYNQSGEICIRGSQIMKGYLNDVAATATTVDTEGWLHTGDVGYVDDDNEI 491

Query: 754  FIVDRVKEIIKFKGFQVPPAELEALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGL 575
            FIVDR KE+IKFKGFQVPPAELE+LL++HPSIADAAVVPQKD+AAGEVPVAFVV+S NGL
Sbjct: 492  FIVDRAKELIKFKGFQVPPAELESLLISHPSIADAAVVPQKDDAAGEVPVAFVVRS-NGL 550

Query: 574  DLTEEAVKDYIAKQVVFYKKLHTVYFIHAIPKSASGKILRKELRARLA 431
            +LTEEAVK++IAKQVVFYK+LH V+F+HAIPKSASGKILRK+LRA+LA
Sbjct: 551  ELTEEAVKEFIAKQVVFYKRLHKVHFVHAIPKSASGKILRKDLRAKLA 598


>gb|ADE96997.1| p-coumarate:CoA-ligase 3 [Sorbus aucuparia]
          Length = 605

 Score =  807 bits (2085), Expect = 0.0
 Identities = 405/588 (68%), Positives = 482/588 (81%), Gaps = 10/588 (1%)
 Frame = -1

Query: 2164 LSPSIIFFTTHHFQTNMSMAAVQSQPIDSEISSLKSPQKPENLKTEHIFKSKLPDIPITN 1985
            L P +I  T+    T +   A  +  IDS  ++             H+F+SKLPDI I N
Sbjct: 21   LMPPLINSTSQQNLTKLQPPACTNNIIDSTTATAT---------INHVFRSKLPDIAIPN 71

Query: 1984 HLPLHSYLFEKLPQVSEKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMIL 1805
            HLPLH+Y F+ LP+ S++PCLI G+TGK YSF+ETHL+ +KTGAGLS LG++KGDVIMIL
Sbjct: 72   HLPLHTYCFQNLPEFSDRPCLIVGSTGKSYSFSETHLISQKTGAGLSNLGIQKGDVIMIL 131

Query: 1804 LPNCPEFVFTFFGTSMIGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEE 1625
            L NC EFVF F G S+IGAVTTTANPFYTT EIFKQVK + AK+I+TQS++V+KL+E   
Sbjct: 132  LQNCAEFVFAFMGASLIGAVTTTANPFYTTAEIFKQVKAANAKLIITQSQYVNKLREHPS 191

Query: 1624 F----------QINQDFRVITIDNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSS 1475
                       ++ +DF+V+TID+PPENCLHFS+LSEANE +LP+  I+ +DPV LPFSS
Sbjct: 192  SADGADQNNYPKLGEDFKVVTIDDPPENCLHFSVLSEANEKELPDVVIDAEDPVALPFSS 251

Query: 1474 GTTGLPKGVILTHKSLSTSVAQQVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALR 1295
            GTTGLPKGVILTHK+L TSVAQQVDGENPN+ L EDDV+LCVLPLFHIFS NSVL+ +LR
Sbjct: 252  GTTGLPKGVILTHKNLVTSVAQQVDGENPNLYLKEDDVVLCVLPLFHIFSFNSVLLCSLR 311

Query: 1294 TGCAILLMPKFEINSLLELIQKHRXXXXXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAA 1115
             G  +LLM KFEI +LLELIQ++R              AKNPMV +FDLSSIR+V+SGAA
Sbjct: 312  AGAGVLLMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKNPMVAEFDLSSIRVVLSGAA 371

Query: 1114 PLGKELEDALRERIPQAVLGQGYGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMK 935
            PLGKELE+AL+ R+PQAVLGQGYGMTEAGPVLSMC AFAKEP+ TK GSCG VVRNAE+K
Sbjct: 372  PLGKELEEALKSRVPQAVLGQGYGMTEAGPVLSMCMAFAKEPMPTKSGSCGTVVRNAELK 431

Query: 934  IVDPETGVSLGYNEAGEICIRGAQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEV 755
            ++DPETG+SLGYN+ GEICIRG QIMK YLND AATA TID EGWLHTGD+GYVD+DDEV
Sbjct: 432  VLDPETGLSLGYNQPGEICIRGFQIMKGYLNDAAATATTIDTEGWLHTGDVGYVDDDDEV 491

Query: 754  FIVDRVKEIIKFKGFQVPPAELEALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGL 575
            FIVDRVKE+IKFKGFQVPPAELE+LL++HPSIADAAVVPQ+D+AAGEVPVAFVV+S +GL
Sbjct: 492  FIVDRVKELIKFKGFQVPPAELESLLISHPSIADAAVVPQRDDAAGEVPVAFVVRS-DGL 550

Query: 574  DLTEEAVKDYIAKQVVFYKKLHTVYFIHAIPKSASGKILRKELRARLA 431
            +LTEEAVK++IAKQVVFYK+LH V+F+HAIPKSASGKILRK+LRA+LA
Sbjct: 551  ELTEEAVKEFIAKQVVFYKRLHKVHFVHAIPKSASGKILRKDLRAKLA 598


>ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis vinifera]
          Length = 570

 Score =  807 bits (2085), Expect = 0.0
 Identities = 402/564 (71%), Positives = 475/564 (84%), Gaps = 4/564 (0%)
 Frame = -1

Query: 2110 MAAVQSQPID-SEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVSE 1934
            M ++++Q  D S + +  S  K  N   +H+F+SKLP+IPI+NHLPLH+Y FE   Q ++
Sbjct: 1    MISIETQNPDVSNLDTSHSIPKMANRIDDHVFRSKLPEIPISNHLPLHTYCFENYSQFAD 60

Query: 1933 KPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMI 1754
            +PCLI G+T K YSFAETHL+ RK GAG + LG+K+GDV+MILL NC EF F+F G SM+
Sbjct: 61   RPCLIVGSTNKTYSFAETHLISRKVGAGFAHLGLKQGDVVMILLQNCAEFAFSFLGASMV 120

Query: 1753 GAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQ---INQDFRVITIDN 1583
            GAVTTTANPFYT+ EIFKQ+  S AKI+VTQ+++VDKL++  + Q   I + F VITID+
Sbjct: 121  GAVTTTANPFYTSAEIFKQLNASKAKIVVTQAQYVDKLRDYPDGQVAKIGEGFTVITIDD 180

Query: 1582 PPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQV 1403
            PPENC+HFS++SEANES+LPE  IN DDPV LPFSSGTTGLPKGV+LTHKSL TSVAQQV
Sbjct: 181  PPENCMHFSVVSEANESELPEVSINSDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQV 240

Query: 1402 DGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHR 1223
            DGENPN++LT DDV+LCVLPLFHI+SLNSVL+ +LR G A+LLM KFEI +LLELIQ++R
Sbjct: 241  DGENPNLHLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELIQRYR 300

Query: 1222 XXXXXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYG 1043
                          AKNPMVE FDLSSIR+V+SGAAPLGKELE ALR R+PQAVLGQGYG
Sbjct: 301  VSVAAVVPPLVLALAKNPMVESFDLSSIRVVLSGAAPLGKELEAALRSRVPQAVLGQGYG 360

Query: 1042 MTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQ 863
            MTEAGPVLSMC AFAK+P  TK GSCG VVRNAE+K+VDPETG SLG N+ GEICIRG Q
Sbjct: 361  MTEAGPVLSMCLAFAKQPFPTKSGSCGTVVRNAELKVVDPETGCSLGRNQPGEICIRGQQ 420

Query: 862  IMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEA 683
            IMK YLNDP ATA+TID++GWLHTGDIGYVD+D+EVFIVDRVKE+IKFKGFQVPPAELEA
Sbjct: 421  IMKGYLNDPEATASTIDVDGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEA 480

Query: 682  LLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTV 503
            LLV+HPSIADAAVVPQKD+ AGEVPVAFVV+S NG +LTEEAVK++I+KQVVFYK+LH V
Sbjct: 481  LLVSHPSIADAAVVPQKDDVAGEVPVAFVVRS-NGFELTEEAVKEFISKQVVFYKRLHKV 539

Query: 502  YFIHAIPKSASGKILRKELRARLA 431
            YF+HAIPKS SGKILRK+LRA+LA
Sbjct: 540  YFVHAIPKSPSGKILRKDLRAKLA 563


>gb|AEX32786.1| coumaroyl-CoA ligase [Vitis vinifera]
          Length = 570

 Score =  806 bits (2081), Expect = 0.0
 Identities = 401/564 (71%), Positives = 474/564 (84%), Gaps = 4/564 (0%)
 Frame = -1

Query: 2110 MAAVQSQPID-SEISSLKSPQKPENLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVSE 1934
            M ++++Q  D S + +  S  K  N   +H+F+SKLP+IPI+NHLPLH+Y FE   Q ++
Sbjct: 1    MISIETQNPDVSNLDTSHSIPKMANRIDDHVFRSKLPEIPISNHLPLHTYCFENYSQFAD 60

Query: 1933 KPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMI 1754
            +PCLI G+T K YSFAETHL+ RK GAG + LG+K+GDV+MILL NC EF F+F G SM+
Sbjct: 61   RPCLIVGSTNKTYSFAETHLISRKVGAGFAHLGLKQGDVVMILLQNCAEFAFSFLGASMV 120

Query: 1753 GAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQ---INQDFRVITIDN 1583
            GAVTTTANPFYT+ EIFKQ+  S AKI+VTQ+++VDKL++  + Q   I + F VITID+
Sbjct: 121  GAVTTTANPFYTSAEIFKQLNASKAKIVVTQAQYVDKLRDYPDGQVAKIGEGFTVITIDD 180

Query: 1582 PPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQV 1403
            PPENC+HFS++SEANES+LPE  IN DDPV LPFSSGTTGLPKGV+LTHKSL TSVAQQV
Sbjct: 181  PPENCMHFSVVSEANESELPEVSINSDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQV 240

Query: 1402 DGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHR 1223
            DGENPN++LT DDV+LCVLPLFHI+SLNSVL+ +LR G A+LLM KFEI +LLELIQ++R
Sbjct: 241  DGENPNLHLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELIQRYR 300

Query: 1222 XXXXXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYG 1043
                          AKNPMVE FDLSSIR+V+SGAAPLGKELE ALR R+PQAVLGQGYG
Sbjct: 301  VSVAAVVPPLVLALAKNPMVESFDLSSIRVVLSGAAPLGKELEAALRSRVPQAVLGQGYG 360

Query: 1042 MTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQ 863
            MTEAGPVLSMC  FAK+P  TK GSCG VVRNAE+K+VDPETG SLG N+ GEICIRG Q
Sbjct: 361  MTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVVDPETGCSLGRNQPGEICIRGQQ 420

Query: 862  IMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEA 683
            IMK YLNDP ATA+TID++GWLHTGDIGYVD+D+EVFIVDRVKE+IKFKGFQVPPAELEA
Sbjct: 421  IMKGYLNDPEATASTIDVDGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEA 480

Query: 682  LLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTV 503
            LLV+HPSIADAAVVPQKD+ AGEVPVAFVV+S NG +LTEEAVK++I+KQVVFYK+LH V
Sbjct: 481  LLVSHPSIADAAVVPQKDDVAGEVPVAFVVRS-NGFELTEEAVKEFISKQVVFYKRLHKV 539

Query: 502  YFIHAIPKSASGKILRKELRARLA 431
            YF+HAIPKS SGKILRK+LRA+LA
Sbjct: 540  YFVHAIPKSPSGKILRKDLRAKLA 563


>ref|XP_006443197.1| hypothetical protein CICLE_v10019532mg [Citrus clementina]
            gi|568850424|ref|XP_006478914.1| PREDICTED:
            4-coumarate--CoA ligase 2-like [Citrus sinensis]
            gi|557545459|gb|ESR56437.1| hypothetical protein
            CICLE_v10019532mg [Citrus clementina]
          Length = 560

 Score =  803 bits (2073), Expect = 0.0
 Identities = 403/563 (71%), Positives = 466/563 (82%), Gaps = 1/563 (0%)
 Frame = -1

Query: 2116 MSMAAVQSQPIDSEISSLKSPQKPENLK-TEHIFKSKLPDIPITNHLPLHSYLFEKLPQV 1940
            M   A +   +  +ISS  +P  P N K   HIFKSKLPDIPI+NHLPLH+Y F+   ++
Sbjct: 1    MISIATKKPELSLDISS-PAPPAPSNEKIATHIFKSKLPDIPISNHLPLHTYCFQD--RL 57

Query: 1939 SEKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTS 1760
            S+ PCLI G TGK YS+AETHL+CRKT AGLS LG+KKGDVIMILL NC EFVF+F G S
Sbjct: 58   SDDPCLIVGLTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGAS 117

Query: 1759 MIGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQINQDFRVITIDNP 1580
            MIGAVTTTANPFYT+ EI KQ + SGAK+I+T S++VD+L ++++     DF VITID P
Sbjct: 118  MIGAVTTTANPFYTSAEILKQFRTSGAKLIITMSQYVDRLPKTDK-----DFTVITIDAP 172

Query: 1579 PENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQVD 1400
            PENCLHF++LSEA+E  +PE  I PDDPV LPFSSGTTGLPKGV+LTHKSL TSVAQQVD
Sbjct: 173  PENCLHFTVLSEADEDQIPEVAIEPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVD 232

Query: 1399 GENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHRX 1220
            GENPN+ LT  DV+LCVLPLFHI+SLNSVL+ +LR G  +LLM KFEI +LLELIQ+HR 
Sbjct: 233  GENPNLYLTNGDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRV 292

Query: 1219 XXXXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYGM 1040
                         AKNPMV  +DLSSIR+V+SGAAPLGKELEDALR R+PQA+LGQGYGM
Sbjct: 293  SVAAVVPPLVLALAKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGM 352

Query: 1039 TEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQI 860
            TEAGPVLSMC  FAK+P  TK GSCG VVRNAE+K++DPE G SL +N+ GEICIRG QI
Sbjct: 353  TEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPEIGASLPHNQPGEICIRGPQI 412

Query: 859  MKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEAL 680
            MK YLNDP ATAATID+EGWLHTGDIGYVD+DDEVFIVDRVKEIIKFKGFQVPPAE+EAL
Sbjct: 413  MKGYLNDPEATAATIDVEGWLHTGDIGYVDDDDEVFIVDRVKEIIKFKGFQVPPAEIEAL 472

Query: 679  LVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTVY 500
            L++HPSI DAAVVPQKDE AGEVPVAFVV+S NG +LTEEA+K+YIAKQVVFYK+LH +Y
Sbjct: 473  LLSHPSIGDAAVVPQKDEVAGEVPVAFVVRS-NGFELTEEAIKEYIAKQVVFYKRLHKIY 531

Query: 499  FIHAIPKSASGKILRKELRARLA 431
            F+HAIPKS SGKILRK+LRA+LA
Sbjct: 532  FVHAIPKSPSGKILRKDLRAKLA 554


>gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]
          Length = 604

 Score =  801 bits (2069), Expect = 0.0
 Identities = 395/544 (72%), Positives = 463/544 (85%), Gaps = 10/544 (1%)
 Frame = -1

Query: 2032 TEHIFKSKLPDIPITNHLPLHSYLFEKLPQVSEKPCLISGATGKIYSFAETHLLCRKTGA 1853
            T H+FKSKLPDIPI NHLPLH+Y F+KL + S++PCLI  +TGK YSF++T+L+ +KTGA
Sbjct: 56   TNHVFKSKLPDIPIPNHLPLHTYCFQKLSEFSDRPCLIVSSTGKSYSFSDTYLISQKTGA 115

Query: 1852 GLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMIGAVTTTANPFYTTPEIFKQVKISGAKI 1673
            GLS LG+KKGDVIMILL NC EFVF F G SMIGA+TTTANPFYT  EIFKQ K S AK+
Sbjct: 116  GLSNLGIKKGDVIMILLQNCAEFVFAFMGASMIGAITTTANPFYTATEIFKQFKNSNAKL 175

Query: 1672 IVTQSKFVDKLKESEE----------FQINQDFRVITIDNPPENCLHFSILSEANESDLP 1523
            I+T S+FVDKL+E+             ++ + F+V+T+D+PPENCLHFS++SEANE +LP
Sbjct: 176  IITHSQFVDKLREAPADGAAPDQNNYLKLGEHFKVVTVDDPPENCLHFSVISEANEKELP 235

Query: 1522 ETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQVDGENPNMNLTEDDVLLCVLP 1343
            E  I+P+DPV LPFSSGTTGLPKGVILTHKSL TSVAQQVDGENPN+ L EDDV+LCVLP
Sbjct: 236  EVVIDPEDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLP 295

Query: 1342 LFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHRXXXXXXXXXXXXXXAKNPMV 1163
            LFHI+SLNSV++ +LR G A+L+M KFEI +LLELIQ++R              AKNPMV
Sbjct: 296  LFHIYSLNSVMLCSLRAGAAVLVMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKNPMV 355

Query: 1162 EKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYGMTEAGPVLSMCPAFAKEPLT 983
             +FDLSSIR+V+SGAAPLGKELE+ALR R+P AVLGQGYGMTEAGPVLSMC AFAKEPL 
Sbjct: 356  AQFDLSSIRVVLSGAAPLGKELEEALRSRVPGAVLGQGYGMTEAGPVLSMCLAFAKEPLP 415

Query: 982  TKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQIMKEYLNDPAATAATIDIEG 803
            +K GSCG V+RNAE+K++D ETG SLGYN+ GEICIRG+QIMK YLND  ATA T+D EG
Sbjct: 416  SKSGSCGTVIRNAELKVIDSETGCSLGYNQPGEICIRGSQIMKGYLNDAEATATTVDKEG 475

Query: 802  WLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEALLVNHPSIADAAVVPQKDEA 623
            WLHTGD+GYVD+D+EVFIVDRVKE+IKFKGFQVPPAELE+LLV+HPSIADAAVVPQKDEA
Sbjct: 476  WLHTGDVGYVDDDEEVFIVDRVKELIKFKGFQVPPAELESLLVSHPSIADAAVVPQKDEA 535

Query: 622  AGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTVYFIHAIPKSASGKILRKELR 443
            AGEVPVAFVV+S NGL LTEEAVK++IAKQVVFYK+LH VYF+HAIPKS SGKILRK+LR
Sbjct: 536  AGEVPVAFVVRS-NGLQLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLR 594

Query: 442  ARLA 431
            A+LA
Sbjct: 595  AKLA 598


>gb|AGW16013.1| 4-coumarate:coenzyme A ligase 1 [Pueraria montana var. lobata]
          Length = 567

 Score =  801 bits (2068), Expect = 0.0
 Identities = 396/549 (72%), Positives = 462/549 (84%), Gaps = 1/549 (0%)
 Frame = -1

Query: 2077 EISSLKSPQKPE-NLKTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVSEKPCLISGATGK 1901
            E+S+ K+ Q    + +T H+FKSKLPDIPI+NH+PLH+Y FEKL   +++PCLI GA  K
Sbjct: 12   EVSTPKTEQNQVCDPQTTHVFKSKLPDIPISNHIPLHAYCFEKLSDFADRPCLIVGAASK 71

Query: 1900 IYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMIGAVTTTANPFY 1721
             Y++AETHL+ RK  AGLS LG++KGDV+MILL N  EFVF+F   SMIGAV TTANPFY
Sbjct: 72   TYTYAETHLISRKIAAGLSNLGIRKGDVVMILLQNSAEFVFSFLAASMIGAVGTTANPFY 131

Query: 1720 TTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQINQDFRVITIDNPPENCLHFSILSEA 1541
            T  EIFKQ  +S AK+I+TQ+ +VDKL+  +   + +DF+V+T+D+PPENCLHFS+LSEA
Sbjct: 132  TAAEIFKQFTVSKAKLIITQAMYVDKLRHHDGTNLGEDFKVVTVDDPPENCLHFSVLSEA 191

Query: 1540 NESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQVDGENPNMNLTEDDV 1361
            NES++PE +I+PDD V +PFSSGTTGLPKGV+LTHKSL+TSVAQQVDGENPN+ LT +DV
Sbjct: 192  NESEVPEVEIHPDDAVAMPFSSGTTGLPKGVVLTHKSLTTSVAQQVDGENPNLYLTTEDV 251

Query: 1360 LLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHRXXXXXXXXXXXXXX 1181
            LLCVLPLFHIFSLNSVL+ ALR G  +LLM KFEI +LLELIQ+HR              
Sbjct: 252  LLCVLPLFHIFSLNSVLLCALRAGSGVLLMQKFEIGTLLELIQRHRVSVAMVVPPLVLAL 311

Query: 1180 AKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYGMTEAGPVLSMCPAF 1001
            AKNPMV  FDLSSIR+V+SGAAPLGKELE+ALR R+PQAVLGQGYGMTEAGPVLSMC  F
Sbjct: 312  AKNPMVADFDLSSIRLVLSGAAPLGKELEEALRNRVPQAVLGQGYGMTEAGPVLSMCLGF 371

Query: 1000 AKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQIMKEYLNDPAATAA 821
            AK+P  TK GSCG VVRNAE+K+VDPETG SLGYN+ GEICIRG QIMK YLND  ATA 
Sbjct: 372  AKQPFPTKSGSCGTVVRNAELKVVDPETGRSLGYNQPGEICIRGQQIMKGYLNDEKATAL 431

Query: 820  TIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEALLVNHPSIADAAVV 641
            TID EGWLHTGD+GYVD+DDE+FIVDRVKE+IKFKGFQVPPAELE LLV+HPSIADAAVV
Sbjct: 432  TIDSEGWLHTGDVGYVDDDDEIFIVDRVKELIKFKGFQVPPAELEGLLVSHPSIADAAVV 491

Query: 640  PQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTVYFIHAIPKSASGKI 461
            PQKD AAGEVPVAFVV+S NG DLTEEAVK++IAKQVVFYK+LH VYF+HAIPKS SGKI
Sbjct: 492  PQKDVAAGEVPVAFVVRS-NGFDLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKI 550

Query: 460  LRKELRARL 434
            LRK+LRA+L
Sbjct: 551  LRKDLRAKL 559


>sp|P31687.2|4CL2_SOYBN RecName: Full=4-coumarate--CoA ligase 2; Short=4CL 2; AltName:
            Full=4-coumaroyl-CoA synthase 2; AltName: Full=Clone
            4CL16 gi|13559169|emb|CAC36095.1| 4-coumarate:Coenzyme A
            ligase isoenzyme 4 [Glycine max]
          Length = 562

 Score =  800 bits (2067), Expect = 0.0
 Identities = 396/549 (72%), Positives = 462/549 (84%), Gaps = 4/549 (0%)
 Frame = -1

Query: 2068 SLKSPQKPENL----KTEHIFKSKLPDIPITNHLPLHSYLFEKLPQVSEKPCLISGATGK 1901
            SL +P+  +N     +T H+FKSKLPDIPI+NHLPLHSY F+ L Q + +PCLI G   K
Sbjct: 7    SLDTPKTDQNQVSDPQTSHVFKSKLPDIPISNHLPLHSYCFQNLSQFAHRPCLIVGPASK 66

Query: 1900 IYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGTSMIGAVTTTANPFY 1721
             +++A+THL+  K  AGLS LG+ KGDV+MILL N  +FVF+F   SMIGAV TTANPFY
Sbjct: 67   TFTYADTHLISSKIAAGLSNLGILKGDVVMILLQNSADFVFSFLAISMIGAVATTANPFY 126

Query: 1720 TTPEIFKQVKISGAKIIVTQSKFVDKLKESEEFQINQDFRVITIDNPPENCLHFSILSEA 1541
            T PEIFKQ  +S AK+I+TQ+ +VDKL+  +  ++ +DF+V+T+D+PPENCLHFS+LSEA
Sbjct: 127  TAPEIFKQFTVSKAKLIITQAMYVDKLRNHDGAKLGEDFKVVTVDDPPENCLHFSVLSEA 186

Query: 1540 NESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQQVDGENPNMNLTEDDV 1361
            NESD+PE +I+PDD V +PFSSGTTGLPKGVILTHKSL+TSVAQQVDGENPN+ LT +DV
Sbjct: 187  NESDVPEVEIHPDDAVAMPFSSGTTGLPKGVILTHKSLTTSVAQQVDGENPNLYLTTEDV 246

Query: 1360 LLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQKHRXXXXXXXXXXXXXX 1181
            LLCVLPLFHIFSLNSVL+ ALR G A+LLM KFEI +LLELIQ+HR              
Sbjct: 247  LLCVLPLFHIFSLNSVLLCALRAGSAVLLMQKFEIGTLLELIQRHRVSVAMVVPPLVLAL 306

Query: 1180 AKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQGYGMTEAGPVLSMCPAF 1001
            AKNPMV  FDLSSIR+V+SGAAPLGKELE+ALR R+PQAVLGQGYGMTEAGPVLSMC  F
Sbjct: 307  AKNPMVADFDLSSIRLVLSGAAPLGKELEEALRNRMPQAVLGQGYGMTEAGPVLSMCLGF 366

Query: 1000 AKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRGAQIMKEYLNDPAATAA 821
            AK+P  TK GSCG VVRNAE+K+VDPETG SLGYN+ GEICIRG QIMK YLND AATA+
Sbjct: 367  AKQPFQTKSGSCGTVVRNAELKVVDPETGRSLGYNQPGEICIRGQQIMKGYLNDEAATAS 426

Query: 820  TIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAELEALLVNHPSIADAAVV 641
            TID EGWLHTGD+GYVD+DDE+FIVDRVKE+IK+KGFQVPPAELE LLV+HPSIADAAVV
Sbjct: 427  TIDSEGWLHTGDVGYVDDDDEIFIVDRVKELIKYKGFQVPPAELEGLLVSHPSIADAAVV 486

Query: 640  PQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLHTVYFIHAIPKSASGKI 461
            PQKD AAGEVPVAFVV+S NG DLTEEAVK++IAKQVVFYK+LH VYF+HAIPKS SGKI
Sbjct: 487  PQKDVAAGEVPVAFVVRS-NGFDLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKI 545

Query: 460  LRKELRARL 434
            LRK+LRA+L
Sbjct: 546  LRKDLRAKL 554


>ref|XP_004307864.1| PREDICTED: LOW QUALITY PROTEIN: 4-coumarate--CoA ligase 2-like
            [Fragaria vesca subsp. vesca]
          Length = 586

 Score =  796 bits (2056), Expect = 0.0
 Identities = 404/575 (70%), Positives = 477/575 (82%), Gaps = 11/575 (1%)
 Frame = -1

Query: 2122 TNMSMAAVQSQPIDSEISSLKSPQKPE-----NLKTEHIFKSKLPDIP-ITNHLPLHSYL 1961
            TN +   V++  I   + S  SP+K        L   H+FKSKLPD+P I+NHLPLH+Y 
Sbjct: 8    TNNTSVVVETADISPTLISA-SPEKQHPATSTTLHDHHVFKSKLPDLPNISNHLPLHTYC 66

Query: 1960 FEKLPQVSEKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFV 1781
            FE +   SE+PCLI G+TGK YSF+ETHL+ +KTGAGLSKLG+ KGDVIMILL NC EFV
Sbjct: 67   FENVSDFSERPCLIVGSTGKTYSFSETHLISQKTGAGLSKLGITKGDVIMILLQNCAEFV 126

Query: 1780 FTFFGTSMIGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLK----ESEEF-QI 1616
            F F G SMIGAVTTTANPFYT  EIFKQ K S AK+I+TQS +VDKL+    +S+++ ++
Sbjct: 127  FAFMGASMIGAVTTTANPFYTASEIFKQFKASNAKLIITQSIYVDKLRHSGPDSDQYPKL 186

Query: 1615 NQDFRVITIDNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTH 1436
             + F+V+TID+PPENCLHFS+LS ANES+LPE  I+P+DPV LPFSSGTTGLPKGVILTH
Sbjct: 187  GEHFQVVTIDDPPENCLHFSVLSGANESELPEVSIDPEDPVALPFSSGTTGLPKGVILTH 246

Query: 1435 KSLSTSVAQQVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEI 1256
            KSL TSVAQQVDGENPN+ L EDDV+LCVLPLFHI+SLNSVL+ +LR G A+L+M KFEI
Sbjct: 247  KSLITSVAQQVDGENPNLYLKEDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLVMHKFEI 306

Query: 1255 NSLLELIQKHRXXXXXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRER 1076
             +LLELIQ++R               +  MV  +DLSSIR+V+SGAAPLGKELE+ALR R
Sbjct: 307  GTLLELIQRYRVXCGSCGATAGDSAGEESMVADYDLSSIRVVLSGAAPLGKELEEALRSR 366

Query: 1075 IPQAVLGQGYGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYN 896
            +PQAVLGQGYGMTEAGPVLSMC AFAK+P  TK GSCG VVRNAE+K+++PETG SLGYN
Sbjct: 367  VPQAVLGQGYGMTEAGPVLSMCLAFAKQPFPTKSGSCGTVVRNAELKVIEPETGRSLGYN 426

Query: 895  EAGEICIRGAQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFK 716
            + GEICIRG+QIMK YLND AATA T+D+EGWLHTGD+GYVD+D EVFIVDRVKE+IKFK
Sbjct: 427  QPGEICIRGSQIMKGYLNDDAATATTVDVEGWLHTGDVGYVDDDGEVFIVDRVKELIKFK 486

Query: 715  GFQVPPAELEALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAK 536
            GFQVPPAELE+LLV+H SIADAAVVPQKD+AAGEVPVAFVV+S NG +LTEEAVK++IAK
Sbjct: 487  GFQVPPAELESLLVSHQSIADAAVVPQKDDAAGEVPVAFVVRS-NGNELTEEAVKEFIAK 545

Query: 535  QVVFYKKLHTVYFIHAIPKSASGKILRKELRARLA 431
            QVVFYK+LH VYF+HAIPKS SGKILRK+LRA+LA
Sbjct: 546  QVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKLA 580


>gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]
          Length = 565

 Score =  795 bits (2054), Expect = 0.0
 Identities = 396/566 (69%), Positives = 478/566 (84%), Gaps = 4/566 (0%)
 Frame = -1

Query: 2116 MSMAAVQSQPIDSEISSLKSPQK-PENLKTEH-IFKSKLPDIPITNHLPLHSYLFEKLPQ 1943
            +S+A+ ++QP       + SP   PEN   E+ IF+SKLPDIPI+NHLPLH+Y FEK+ +
Sbjct: 2    LSVASPETQP------QIPSPTPTPENPPVENFIFRSKLPDIPISNHLPLHTYCFEKITE 55

Query: 1942 VSEKPCLISGATGKIYSFAETHLLCRKTGAGLSKLGVKKGDVIMILLPNCPEFVFTFFGT 1763
             S+ PCLISG++G+IY+FAETHL+C+K+ AGLSKLG+KKGDVIMILL N PEF F+F G 
Sbjct: 56   FSDAPCLISGSSGRIYTFAETHLICQKSAAGLSKLGIKKGDVIMILLQNSPEFAFSFMGA 115

Query: 1762 SMIGAVTTTANPFYTTPEIFKQVKISGAKIIVTQSKFVDKLKES-EEF-QINQDFRVITI 1589
            SMIGA+TTTANPF T+ EIFKQ  +SGAK+++TQS++VDKL+++ E+F +I +DF VIT+
Sbjct: 116  SMIGAITTTANPFCTSAEIFKQFSVSGAKLVITQSQYVDKLRDTGEKFPKIGEDFTVITV 175

Query: 1588 DNPPENCLHFSILSEANESDLPETKINPDDPVVLPFSSGTTGLPKGVILTHKSLSTSVAQ 1409
            D+PPE+CLHFS++S A+E ++P   I+PDDPV +PFSSGTTGLPKGV LTHKSL +SVAQ
Sbjct: 176  DDPPESCLHFSVISGADEREIPSVSIDPDDPVSMPFSSGTTGLPKGVFLTHKSLISSVAQ 235

Query: 1408 QVDGENPNMNLTEDDVLLCVLPLFHIFSLNSVLMIALRTGCAILLMPKFEINSLLELIQK 1229
            QVDGENPN+ L  DD++LCVLPLFHI+SLNSVL+ +LR G  +LLM KFEI SLLELIQ+
Sbjct: 236  QVDGENPNLYLKTDDIVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMHKFEIRSLLELIQR 295

Query: 1228 HRXXXXXXXXXXXXXXAKNPMVEKFDLSSIRIVMSGAAPLGKELEDALRERIPQAVLGQG 1049
            HR              AKNPMV  FDLSSIRIV+SGAAPLGKELE+AL  R+PQA+ GQG
Sbjct: 296  HRVSVAPVVPPLVLALAKNPMVASFDLSSIRIVLSGAAPLGKELEEALHSRLPQAIFGQG 355

Query: 1048 YGMTEAGPVLSMCPAFAKEPLTTKPGSCGVVVRNAEMKIVDPETGVSLGYNEAGEICIRG 869
            YGMTEAGPVLSMC  FA++   TK GSCG VVRNAE+K++DPETG SL YN+ GEICIRG
Sbjct: 356  YGMTEAGPVLSMCLGFARQAFPTKSGSCGTVVRNAELKVIDPETGFSLQYNQPGEICIRG 415

Query: 868  AQIMKEYLNDPAATAATIDIEGWLHTGDIGYVDNDDEVFIVDRVKEIIKFKGFQVPPAEL 689
             QIMK YLND  ATA+TID++GWLHTGD+GYVD+DDEVFIVDRVKE+IKFKGFQVPPAEL
Sbjct: 416  PQIMKGYLNDAEATASTIDVDGWLHTGDVGYVDDDDEVFIVDRVKELIKFKGFQVPPAEL 475

Query: 688  EALLVNHPSIADAAVVPQKDEAAGEVPVAFVVKSTNGLDLTEEAVKDYIAKQVVFYKKLH 509
            EALL++HPSIADAAVVPQKDEAAGEVPVAFVV+S +G +LTEEAVK++IAKQVVFYK+LH
Sbjct: 476  EALLISHPSIADAAVVPQKDEAAGEVPVAFVVRS-SGFELTEEAVKEFIAKQVVFYKRLH 534

Query: 508  TVYFIHAIPKSASGKILRKELRARLA 431
             VYF+HAIPKSASGKIL K+LRA+LA
Sbjct: 535  KVYFVHAIPKSASGKILGKDLRAKLA 560