BLASTX nr result
ID: Achyranthes22_contig00012444
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Achyranthes22_contig00012444
(1438 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EXB88697.1| hypothetical protein L484_015382 [Morus notabilis] 362 3e-97
gb|EOY08252.1| Soul heme-binding family protein isoform 1 [Theob... 361 5e-97
ref|XP_006481768.1| PREDICTED: heme-binding-like protein At3g101... 358 2e-96
ref|XP_006430192.1| hypothetical protein CICLE_v10012335mg [Citr... 356 1e-95
ref|XP_002528755.1| protein with unknown function [Ricinus commu... 356 1e-95
ref|XP_002282544.1| PREDICTED: heme-binding-like protein At3g101... 352 2e-94
ref|XP_004305335.1| PREDICTED: heme-binding-like protein At3g101... 345 2e-92
emb|CBI30300.3| unnamed protein product [Vitis vinifera] 345 3e-92
ref|XP_004149204.1| PREDICTED: heme-binding-like protein At3g101... 345 4e-92
ref|XP_004249441.1| PREDICTED: heme-binding-like protein At3g101... 343 8e-92
ref|XP_003554437.1| PREDICTED: heme-binding-like protein At3g101... 342 2e-91
emb|CAN62765.1| hypothetical protein VITISV_021815 [Vitis vinifera] 342 3e-91
ref|XP_003625826.1| Heme-binding-like protein [Medicago truncatu... 341 4e-91
ref|XP_006339129.1| PREDICTED: heme-binding-like protein At3g101... 339 1e-90
gb|ESW34900.1| hypothetical protein PHAVU_001G190400g [Phaseolus... 338 3e-90
ref|XP_002882654.1| soul heme-binding family protein [Arabidopsi... 338 3e-90
ref|XP_006279664.1| hypothetical protein CARUB_v10026829mg [Caps... 336 1e-89
gb|EMJ03675.1| hypothetical protein PRUPE_ppa010378mg [Prunus pe... 336 1e-89
ref|XP_004494187.1| PREDICTED: heme-binding-like protein At3g101... 334 5e-89
ref|XP_006407606.1| hypothetical protein EUTSA_v10021175mg [Eutr... 333 1e-88
>gb|EXB88697.1| hypothetical protein L484_015382 [Morus notabilis]
Length = 277
Score = 362 bits (928), Expect = 3e-97
Identities = 184/274 (67%), Positives = 221/274 (80%), Gaps = 3/274 (1%)
Frame = +3
Query: 63 IISLATNNSKSMAKP--QQQRQSMSAFDARVXXXXXXXXXXXXXXXRVVADLVSETSKYV 236
I S+ N + +++ P QQ+R++MSA +AR+ R++ADLV+ETSKYV
Sbjct: 3 ISSMVANRATALSPPSQQQRRRTMSALEARISLVFALASQTSSVSLRLLADLVTETSKYV 62
Query: 237 VPKKFEARNLEEAFMAVPDLETVNFKVLSRKDQYEIREVEPYFVAETTMPGREGFDFFGA 416
PK+FE+R LEEA M+VPDLETVNF+VLSR DQYEIREVEPYF+AETTMPGR GFDF GA
Sbjct: 63 FPKRFESRTLEEALMSVPDLETVNFRVLSRTDQYEIREVEPYFIAETTMPGRTGFDFNGA 122
Query: 417 SQSFNVLAEYLFGKNIPIEKMEMTTPVITRKTS-EGERMEMTTPVITRRVGRQEQWTMSF 593
SQSFNVLAEYLFGKN EKMEMTTPVITRK GE+MEMTTPVIT+RV Q++W MSF
Sbjct: 123 SQSFNVLAEYLFGKNTTREKMEMTTPVITRKAQPNGEKMEMTTPVITKRVEDQDKWQMSF 182
Query: 594 VMPSKYGSNLPVPKDSEVRITEVPKKIVAVAAFSGYVTDEEVQRREFKLRDALKSDLEFK 773
VMPSKYG +LP+PKD VRI EVP+K +AV AFSG+VTDEEV++RE KLR+ALK+D +F+
Sbjct: 183 VMPSKYGGDLPLPKDPSVRIKEVPRKTIAVVAFSGFVTDEEVRQRENKLREALKNDRKFQ 242
Query: 774 VKVGAEVEVLQYNPPFTLPFTRRNEIAVEVERKK 875
VK G VEV QYNPPFTLPF RRNEIA+EVE+++
Sbjct: 243 VKEGTSVEVAQYNPPFTLPFQRRNEIALEVEKRE 276
>gb|EOY08252.1| Soul heme-binding family protein isoform 1 [Theobroma cacao]
Length = 309
Score = 361 bits (926), Expect = 5e-97
Identities = 184/273 (67%), Positives = 221/273 (80%), Gaps = 1/273 (0%)
Frame = +3
Query: 60 LIISLATNNSKSMAKPQQQRQSMSAFDARVXXXXXXXXXXXXXXXRVVADLVSETSKYVV 239
++++ T + ++A PQ R+++SA +AR+ R++ DL SET+KYV
Sbjct: 38 MVMTERTATTTTLAPPQ--RRTVSASEARISLVFALASQASSVSQRLLMDLASETAKYVF 95
Query: 240 PKKFEARNLEEAFMAVPDLETVNFKVLSRKDQYEIREVEPYFVAETTMPGREGFDFFGAS 419
PK+FE+RNLEEA MAVPDLETV FKVLSR DQYEIR VEPYF+AETTMPG+ GFDF GAS
Sbjct: 96 PKRFESRNLEEALMAVPDLETVRFKVLSRTDQYEIRVVEPYFIAETTMPGKTGFDFNGAS 155
Query: 420 QSFNVLAEYLFGKNIPIEKMEMTTPVITRKT-SEGERMEMTTPVITRRVGRQEQWTMSFV 596
QSFNVLAEYLFGKN E MEMTTPV T +T S+GERMEMTTPVIT++V +Q +W MSFV
Sbjct: 156 QSFNVLAEYLFGKNTSKETMEMTTPVFTSRTQSDGERMEMTTPVITKKVEKQGKWQMSFV 215
Query: 597 MPSKYGSNLPVPKDSEVRITEVPKKIVAVAAFSGYVTDEEVQRREFKLRDALKSDLEFKV 776
MPSKYGSN+P+PKD VRI EVP+K+VAV AFSG+VTDEEV+RRE KLRDALK+D +F+V
Sbjct: 216 MPSKYGSNVPLPKDPSVRIKEVPRKVVAVVAFSGFVTDEEVKRRELKLRDALKNDRQFQV 275
Query: 777 KVGAEVEVLQYNPPFTLPFTRRNEIAVEVERKK 875
K GA VEV QYNPPFTLPFTRRNEIA+EVE+K+
Sbjct: 276 KEGASVEVAQYNPPFTLPFTRRNEIALEVEKKE 308
>ref|XP_006481768.1| PREDICTED: heme-binding-like protein At3g10130, chloroplastic-like
[Citrus sinensis]
Length = 296
Score = 358 bits (920), Expect = 2e-96
Identities = 185/275 (67%), Positives = 219/275 (79%), Gaps = 1/275 (0%)
Frame = +3
Query: 54 LPLIISLATNNSKSMAKPQQQRQSMSAFDARVXXXXXXXXXXXXXXXRVVADLVSETSKY 233
L +I S+ TNN +R+ +SAF+ARV R++ DL +ET+KY
Sbjct: 30 LTIINSITTNN---------ERKPISAFEARVSLVFALASQTSSLSQRLLMDLATETAKY 80
Query: 234 VVPKKFEARNLEEAFMAVPDLETVNFKVLSRKDQYEIREVEPYFVAETTMPGREGFDFFG 413
V PK+FE++NLEEA MAVPDLETV FKVLSR+ QYEIREVE YF+AET MPGR GFDF G
Sbjct: 81 VFPKRFESQNLEEALMAVPDLETVKFKVLSRRGQYEIREVESYFIAETMMPGRTGFDFNG 140
Query: 414 ASQSFNVLAEYLFGKNIPIEKMEMTTPVITRKT-SEGERMEMTTPVITRRVGRQEQWTMS 590
AS+SFNVLAEYLFGKN E MEMTTPVITRKT S+GE+MEMTTPVI++++ Q++W MS
Sbjct: 141 ASRSFNVLAEYLFGKNTKRETMEMTTPVITRKTQSDGEKMEMTTPVISKKLEDQDKWQMS 200
Query: 591 FVMPSKYGSNLPVPKDSEVRITEVPKKIVAVAAFSGYVTDEEVQRREFKLRDALKSDLEF 770
FVMPSKYG+NLP+PKD VRI EVPKK+VAV AFSG+VTDE+V+RRE KLRDALK D +F
Sbjct: 201 FVMPSKYGANLPLPKDPSVRIKEVPKKVVAVVAFSGFVTDEDVKRRELKLRDALKGDRQF 260
Query: 771 KVKVGAEVEVLQYNPPFTLPFTRRNEIAVEVERKK 875
+VK GA VEV QYNPPFTLPFTRRNEIA+EVERK+
Sbjct: 261 RVKEGASVEVAQYNPPFTLPFTRRNEIALEVERKE 295
>ref|XP_006430192.1| hypothetical protein CICLE_v10012335mg [Citrus clementina]
gi|557532249|gb|ESR43432.1| hypothetical protein
CICLE_v10012335mg [Citrus clementina]
Length = 296
Score = 356 bits (914), Expect = 1e-95
Identities = 187/293 (63%), Positives = 222/293 (75%), Gaps = 7/293 (2%)
Frame = +3
Query: 18 LLSPSLKM-AGAKLPLIISLATNNSKSMA-----KPQQQRQSMSAFDARVXXXXXXXXXX 179
L PSL + LP ++L S+ +R+ SAF+ARV
Sbjct: 3 LCKPSLSLITPCTLPKTLTLKPTKKPSLTITNSITTNNERKPTSAFEARVSLVFALASQT 62
Query: 180 XXXXXRVVADLVSETSKYVVPKKFEARNLEEAFMAVPDLETVNFKVLSRKDQYEIREVEP 359
R++ DL +ET+KYV PK+FE++NLEEA MAVPDLETV FKVLSR+ QYEIREVE
Sbjct: 63 SSLSQRLLMDLATETAKYVFPKRFESQNLEEALMAVPDLETVKFKVLSRRGQYEIREVES 122
Query: 360 YFVAETTMPGREGFDFFGASQSFNVLAEYLFGKNIPIEKMEMTTPVITRKT-SEGERMEM 536
YF+AETTMPGR GFD GAS+SFNVLAEYLFGKN E MEMTTPVITRKT S+GE+MEM
Sbjct: 123 YFIAETTMPGRTGFDLNGASRSFNVLAEYLFGKNTKRETMEMTTPVITRKTQSDGEKMEM 182
Query: 537 TTPVITRRVGRQEQWTMSFVMPSKYGSNLPVPKDSEVRITEVPKKIVAVAAFSGYVTDEE 716
TTPVI++++ Q++W MSFVMPSKYG+NLP+PKD VRI EVPKK+VAV AFSG+VTDEE
Sbjct: 183 TTPVISKKLEDQDEWQMSFVMPSKYGANLPLPKDPSVRIKEVPKKVVAVVAFSGFVTDEE 242
Query: 717 VQRREFKLRDALKSDLEFKVKVGAEVEVLQYNPPFTLPFTRRNEIAVEVERKK 875
V++RE KLRDALK D +F+VK GA VEV QYNPPFTLPFTRRNEIA+EVERK+
Sbjct: 243 VKQRELKLRDALKGDRQFRVKEGASVEVAQYNPPFTLPFTRRNEIALEVERKE 295
>ref|XP_002528755.1| protein with unknown function [Ricinus communis]
gi|223531849|gb|EEF33667.1| protein with unknown
function [Ricinus communis]
Length = 297
Score = 356 bits (914), Expect = 1e-95
Identities = 179/280 (63%), Positives = 224/280 (80%), Gaps = 1/280 (0%)
Frame = +3
Query: 39 MAGAKLPLIISLATNNSKSMAKPQQQRQSMSAFDARVXXXXXXXXXXXXXXXRVVADLVS 218
+ + +I S+ T+ + + A+P+ + + SAF+ARV R++ DL +
Sbjct: 19 LTASSTTIIKSMVTDRTATSAQPR--KATSSAFEARVSLIFALASQTSNVSQRLLFDLAN 76
Query: 219 ETSKYVVPKKFEARNLEEAFMAVPDLETVNFKVLSRKDQYEIREVEPYFVAETTMPGREG 398
ET+KYV PK+F +RNLEEA MAVPDLETV +KVLSR+DQYEIREVEPYF+AETTMPG+ G
Sbjct: 77 ETAKYVFPKRFASRNLEEALMAVPDLETVEYKVLSRRDQYEIREVEPYFIAETTMPGKTG 136
Query: 399 FDFFGASQSFNVLAEYLFGKNIPIEKMEMTTPVITRKT-SEGERMEMTTPVITRRVGRQE 575
FD GASQSFNVLAEYLFGKN+ EKMEMTTPV TRK S+GE+MEMTTPVIT++V Q+
Sbjct: 137 FDLNGASQSFNVLAEYLFGKNVTKEKMEMTTPVFTRKVQSDGEKMEMTTPVITKKVEDQD 196
Query: 576 QWTMSFVMPSKYGSNLPVPKDSEVRITEVPKKIVAVAAFSGYVTDEEVQRREFKLRDALK 755
+W MSFVMPSKYG++LP+PKD V+I EVPKK+VAV AFSG+VTDE+V++RE KLR+ALK
Sbjct: 197 KWQMSFVMPSKYGADLPLPKDKTVKIKEVPKKVVAVVAFSGFVTDEDVKQRELKLRNALK 256
Query: 756 SDLEFKVKVGAEVEVLQYNPPFTLPFTRRNEIAVEVERKK 875
+D F+VK GA +EV QYNPPFTLPFTRRNEIA+E+ERK+
Sbjct: 257 NDPLFRVKKGASMEVAQYNPPFTLPFTRRNEIAIEIERKE 296
>ref|XP_002282544.1| PREDICTED: heme-binding-like protein At3g10130, chloroplastic-like
[Vitis vinifera]
Length = 285
Score = 352 bits (904), Expect = 2e-94
Identities = 180/288 (62%), Positives = 223/288 (77%), Gaps = 1/288 (0%)
Frame = +3
Query: 18 LLSPSLKMAGAKLPLIISLATNNSKSMAKPQQQRQSMSAFDARVXXXXXXXXXXXXXXXR 197
LL L M G ++ S +N ++ QR++MS F+ARV R
Sbjct: 2 LLHRPLVMPG----VLYSRNSNGYRASLSSSPQRRAMSGFEARVSLVLALVSQASSVSQR 57
Query: 198 VVADLVSETSKYVVPKKFEARNLEEAFMAVPDLETVNFKVLSRKDQYEIREVEPYFVAET 377
++ DL SET+KY P++FE+ NLEEA M+VPDLETV FKVLSRKDQYEIREVEPYF+AET
Sbjct: 58 LLMDLASETTKYAFPRRFESSNLEEALMSVPDLETVKFKVLSRKDQYEIREVEPYFIAET 117
Query: 378 TMPGREGFDFFGASQSFNVLAEYLFGKNIPIEKMEMTTPVITRKT-SEGERMEMTTPVIT 554
TMPG+ GFDF G+SQSFNVLAEYLFGKN E+MEMTTPV TR+ S+G++MEMTTPVIT
Sbjct: 118 TMPGKNGFDFNGSSQSFNVLAEYLFGKNTTKERMEMTTPVFTRRDQSDGKKMEMTTPVIT 177
Query: 555 RRVGRQEQWTMSFVMPSKYGSNLPVPKDSEVRITEVPKKIVAVAAFSGYVTDEEVQRREF 734
+++ Q++W MSFVMP+KYG++LP+PKD VRI EVP+KIVAV AFSG+VTDEEV+ RE
Sbjct: 178 KKLQDQDKWQMSFVMPAKYGADLPLPKDQSVRIKEVPRKIVAVVAFSGFVTDEEVKLRES 237
Query: 735 KLRDALKSDLEFKVKVGAEVEVLQYNPPFTLPFTRRNEIAVEVERKKI 878
KLR AL++D +F+VK GA VEV QYNPPFTLPFTRRNE+A+EVERK++
Sbjct: 238 KLRSALRNDKQFQVKEGAPVEVAQYNPPFTLPFTRRNEVALEVERKQV 285
>ref|XP_004305335.1| PREDICTED: heme-binding-like protein At3g10130, chloroplastic-like
[Fragaria vesca subsp. vesca]
Length = 270
Score = 345 bits (886), Expect = 2e-92
Identities = 171/258 (66%), Positives = 210/258 (81%), Gaps = 1/258 (0%)
Frame = +3
Query: 105 PQQQRQSMSAFDARVXXXXXXXXXXXXXXXRVVADLVSETSKYVVPKKFEARNLEEAFMA 284
P Q+R++MSA +ARV R++ DL +ET+KYV PK+FEA+ LEEA M+
Sbjct: 11 PPQRRRTMSALEARVSLVAALAAQASAVSQRLLVDLATETAKYVFPKQFEAQTLEEALMS 70
Query: 285 VPDLETVNFKVLSRKDQYEIREVEPYFVAETTMPGREGFDFFGASQSFNVLAEYLFGKNI 464
VPDLETV +KVLSR+DQYEIREVEPYF+AE TMPG+ GFDF GASQSFNVLAEYLFGKN
Sbjct: 71 VPDLETVKYKVLSRRDQYEIREVEPYFIAEATMPGKTGFDFNGASQSFNVLAEYLFGKNT 130
Query: 465 PIEKMEMTTPVITRKT-SEGERMEMTTPVITRRVGRQEQWTMSFVMPSKYGSNLPVPKDS 641
EKMEMTTPV TRK S+GE+MEMTTPVIT+RV +++W MSFVMPSKYG+NLP+PKD
Sbjct: 131 KQEKMEMTTPVFTRKAQSDGEKMEMTTPVITKRVEDEDKWKMSFVMPSKYGANLPLPKDP 190
Query: 642 EVRITEVPKKIVAVAAFSGYVTDEEVQRREFKLRDALKSDLEFKVKVGAEVEVLQYNPPF 821
V++ EVP+K+VAV AFSG+VTDE +++RE KLR+AL++D +F+VK G VEV QYNPPF
Sbjct: 191 SVKVKEVPRKLVAVVAFSGFVTDEVIKQRESKLREALENDRQFQVKEGTAVEVAQYNPPF 250
Query: 822 TLPFTRRNEIAVEVERKK 875
TLPF RRNEI++EVE K+
Sbjct: 251 TLPFQRRNEISLEVESKE 268
>emb|CBI30300.3| unnamed protein product [Vitis vinifera]
Length = 252
Score = 345 bits (885), Expect = 3e-92
Identities = 171/252 (67%), Positives = 207/252 (82%), Gaps = 1/252 (0%)
Frame = +3
Query: 126 MSAFDARVXXXXXXXXXXXXXXXRVVADLVSETSKYVVPKKFEARNLEEAFMAVPDLETV 305
MS F+ARV R++ DL SET+KY P++FE+ NLEEA M+VPDLETV
Sbjct: 1 MSGFEARVSLVLALVSQASSVSQRLLMDLASETTKYAFPRRFESSNLEEALMSVPDLETV 60
Query: 306 NFKVLSRKDQYEIREVEPYFVAETTMPGREGFDFFGASQSFNVLAEYLFGKNIPIEKMEM 485
FKVLSRKDQYEIREVEPYF+AETTMPG+ GFDF G+SQSFNVLAEYLFGKN E+MEM
Sbjct: 61 KFKVLSRKDQYEIREVEPYFIAETTMPGKNGFDFNGSSQSFNVLAEYLFGKNTTKERMEM 120
Query: 486 TTPVITRKT-SEGERMEMTTPVITRRVGRQEQWTMSFVMPSKYGSNLPVPKDSEVRITEV 662
TTPV TR+ S+G++MEMTTPVIT+++ Q++W MSFVMP+KYG++LP+PKD VRI EV
Sbjct: 121 TTPVFTRRDQSDGKKMEMTTPVITKKLQDQDKWQMSFVMPAKYGADLPLPKDQSVRIKEV 180
Query: 663 PKKIVAVAAFSGYVTDEEVQRREFKLRDALKSDLEFKVKVGAEVEVLQYNPPFTLPFTRR 842
P+KIVAV AFSG+VTDEEV+ RE KLR AL++D +F+VK GA VEV QYNPPFTLPFTRR
Sbjct: 181 PRKIVAVVAFSGFVTDEEVKLRESKLRSALRNDKQFQVKEGAPVEVAQYNPPFTLPFTRR 240
Query: 843 NEIAVEVERKKI 878
NE+A+EVERK++
Sbjct: 241 NEVALEVERKQV 252
>ref|XP_004149204.1| PREDICTED: heme-binding-like protein At3g10130, chloroplastic-like
[Cucumis sativus] gi|449500911|ref|XP_004161228.1|
PREDICTED: heme-binding-like protein At3g10130,
chloroplastic-like [Cucumis sativus]
Length = 305
Score = 345 bits (884), Expect = 4e-92
Identities = 172/285 (60%), Positives = 222/285 (77%), Gaps = 1/285 (0%)
Frame = +3
Query: 27 PSLKMAGAKLPLIISLATNNSKSMAKPQQQRQSMSAFDARVXXXXXXXXXXXXXXXRVVA 206
PS+ + +++ +T + + Q R+++SA +AR+ RV+
Sbjct: 21 PSITRRRSSSTSVVADSTVRNSTDVATSQSRRTISAQEARISLVFALASQASTLSQRVLL 80
Query: 207 DLVSETSKYVVPKKFEARNLEEAFMAVPDLETVNFKVLSRKDQYEIREVEPYFVAETTMP 386
+L SETSKY++PK+F++RNLEEA MAVPDLET+ FKVLSR+D+YEIREVEPYF+AETTMP
Sbjct: 81 ELASETSKYLLPKRFDSRNLEEALMAVPDLETLKFKVLSRRDEYEIREVEPYFIAETTMP 140
Query: 387 GREGFDFFGASQSFNVLAEYLFGKNIPIEKMEMTTPVITRK-TSEGERMEMTTPVITRRV 563
G+ GFDF GASQSFNVLA YLFGKN EKMEMTTPV+T + S+GE+M+ TTPVIT+ V
Sbjct: 141 GKSGFDFGGASQSFNVLAAYLFGKNKAREKMEMTTPVLTSQYKSDGEKMDTTTPVITKNV 200
Query: 564 GRQEQWTMSFVMPSKYGSNLPVPKDSEVRITEVPKKIVAVAAFSGYVTDEEVQRREFKLR 743
++QW MSFV+PSKYG N PVP+D+ VRI EVP+KI+AV AFSG+VTD+EV++RE +LR
Sbjct: 201 DGKDQWKMSFVIPSKYGQNFPVPQDTSVRIQEVPRKILAVVAFSGFVTDDEVKKRESRLR 260
Query: 744 DALKSDLEFKVKVGAEVEVLQYNPPFTLPFTRRNEIAVEVERKKI 878
DALK+D EF+VK G+ VEV QYNPPFTLPF RRNEIA+EVE+K++
Sbjct: 261 DALKNDKEFQVKAGSFVEVAQYNPPFTLPFQRRNEIALEVEKKEV 305
>ref|XP_004249441.1| PREDICTED: heme-binding-like protein At3g10130, chloroplastic-like
[Solanum lycopersicum]
Length = 294
Score = 343 bits (881), Expect = 8e-92
Identities = 187/293 (63%), Positives = 218/293 (74%), Gaps = 7/293 (2%)
Frame = +3
Query: 18 LLSPS-----LKMAGAKLPLIISLATNNSKSMAKPQQQRQSMSAFDARVXXXXXXXXXXX 182
LLSPS L + + I S+A N S S P Q+R MSA +AR+
Sbjct: 3 LLSPSSIFAPLSTSRIRPSPIKSMALNRSSSA--PPQRRNGMSALEARISLVIALASQTS 60
Query: 183 XXXXRVVADLVSETSKYVVPKK-FEARNLEEAFMAVPDLETVNFKVLSRKDQYEIREVEP 359
+++ +L SET+KYV+PK+ FE+RNLEEA M+VPDLETV FKVL DQYEIREVEP
Sbjct: 61 SLSQKLLTELASETAKYVLPKRIFESRNLEEALMSVPDLETVKFKVLKLTDQYEIREVEP 120
Query: 360 YFVAETTMPGREGFDFFGASQSFNVLAEYLFGKNIPIEKMEMTTPVITRKT-SEGERMEM 536
YFVAE TMPG+ GFDF GASQSFN LAEYLFGKN E M MTTPVITR+T S+GE+MEM
Sbjct: 121 YFVAEATMPGKSGFDFNGASQSFNTLAEYLFGKNTKKESMAMTTPVITRRTQSDGEKMEM 180
Query: 537 TTPVITRRVGRQEQWTMSFVMPSKYGSNLPVPKDSEVRITEVPKKIVAVAAFSGYVTDEE 716
TTPVIT+R QE+W MSFVMPSKYGS+LP+PKDS V I EVP+K VAV AFSG+V DEE
Sbjct: 181 TTPVITKRAEDQEKWRMSFVMPSKYGSDLPLPKDSSVTIKEVPRKTVAVVAFSGFVNDEE 240
Query: 717 VQRREFKLRDALKSDLEFKVKVGAEVEVLQYNPPFTLPFTRRNEIAVEVERKK 875
V+ RE +LR ALK D EF+VK GA VEV QYNPPFTLPFTRRNEI++EVE K+
Sbjct: 241 VKARESRLRTALKGDAEFQVKDGALVEVAQYNPPFTLPFTRRNEISLEVEMKQ 293
>ref|XP_003554437.1| PREDICTED: heme-binding-like protein At3g10130, chloroplastic-like
isoform X1 [Glycine max]
Length = 303
Score = 342 bits (878), Expect = 2e-91
Identities = 173/272 (63%), Positives = 213/272 (78%), Gaps = 4/272 (1%)
Frame = +3
Query: 69 SLATNNSKSMAK---PQQQRQSMSAFDARVXXXXXXXXXXXXXXXRVVADLVSETSKYVV 239
+L+ NS S + +R+++S F++R+ R++AD+ +ET+KYV
Sbjct: 30 NLSVTNSASSGERISSSTRRRTISPFESRISLIFALASQANSLSQRLLADVAAETAKYVF 89
Query: 240 PKKFEARNLEEAFMAVPDLETVNFKVLSRKDQYEIREVEPYFVAETTMPGREGFDFFGAS 419
PK+FE+RNLEEA M+VPDLETV+FKVLSR DQYEIREVEPYFVAETTMPG+ GFDF GAS
Sbjct: 90 PKRFESRNLEEALMSVPDLETVDFKVLSRMDQYEIREVEPYFVAETTMPGKSGFDFNGAS 149
Query: 420 QSFNVLAEYLFGKNIPIEKMEMTTPVITRKT-SEGERMEMTTPVITRRVGRQEQWTMSFV 596
+SFN LAEYLFGKN EKMEMTTPV T K S+G +M+MTTPV+T ++ Q+ W MSFV
Sbjct: 150 RSFNALAEYLFGKNTTKEKMEMTTPVFTSKNQSDGVKMDMTTPVLTTKMEDQDNWKMSFV 209
Query: 597 MPSKYGSNLPVPKDSEVRITEVPKKIVAVAAFSGYVTDEEVQRREFKLRDALKSDLEFKV 776
MPSKYG+NLP+PKDS VRI EVP+KIVAV +FSG+V DEE+++RE KLRDALKSD +F++
Sbjct: 210 MPSKYGANLPLPKDSSVRIKEVPRKIVAVVSFSGFVNDEEIKQRELKLRDALKSDSQFEI 269
Query: 777 KVGAEVEVLQYNPPFTLPFTRRNEIAVEVERK 872
K G VEV QYNPPFTLPF RRNEIA+EVE K
Sbjct: 270 KEGTSVEVAQYNPPFTLPFQRRNEIALEVEWK 301
>emb|CAN62765.1| hypothetical protein VITISV_021815 [Vitis vinifera]
Length = 252
Score = 342 bits (876), Expect = 3e-91
Identities = 170/252 (67%), Positives = 204/252 (80%), Gaps = 1/252 (0%)
Frame = +3
Query: 126 MSAFDARVXXXXXXXXXXXXXXXRVVADLVSETSKYVVPKKFEARNLEEAFMAVPDLETV 305
MS F+ARV R++ DL SET KY P++FE+ NLEEA M+VPDLETV
Sbjct: 1 MSGFEARVSLVLALVSQASSVSQRLLMDLASETXKYAFPRRFESSNLEEALMSVPDLETV 60
Query: 306 NFKVLSRKDQYEIREVEPYFVAETTMPGREGFDFFGASQSFNVLAEYLFGKNIPIEKMEM 485
FKVLSRKDQYEIREVEPYF+AETTMPG+ GFDF G+SQSFNVLAEYLFGKN E+MEM
Sbjct: 61 KFKVLSRKDQYEIREVEPYFIAETTMPGKNGFDFNGSSQSFNVLAEYLFGKNTTKERMEM 120
Query: 486 TTPVITRKT-SEGERMEMTTPVITRRVGRQEQWTMSFVMPSKYGSNLPVPKDSEVRITEV 662
TTPV TR+ S+G +MEMTTPVIT+++ Q++W MSFVMP+KYG++LP+PKD VRI EV
Sbjct: 121 TTPVFTRRDQSDGXKMEMTTPVITKKLQDQDKWQMSFVMPAKYGADLPLPKDQSVRIKEV 180
Query: 663 PKKIVAVAAFSGYVTDEEVQRREFKLRDALKSDLEFKVKVGAEVEVLQYNPPFTLPFTRR 842
P+KIVAV AFSG+VTDEEV+ RE KLR AL++D +F+VK GA VEV QYNPPFTLPF RR
Sbjct: 181 PRKIVAVVAFSGFVTDEEVKLRESKLRSALRNDKQFQVKEGAPVEVAQYNPPFTLPFARR 240
Query: 843 NEIAVEVERKKI 878
NE+A+EVERK++
Sbjct: 241 NEVALEVERKQV 252
>ref|XP_003625826.1| Heme-binding-like protein [Medicago truncatula]
gi|355500841|gb|AES82044.1| Heme-binding-like protein
[Medicago truncatula]
Length = 296
Score = 341 bits (875), Expect = 4e-91
Identities = 175/291 (60%), Positives = 222/291 (76%), Gaps = 6/291 (2%)
Frame = +3
Query: 18 LLSPSLKMAGAKLPL-----IISLATNNSKSMAKPQQQRQSMSAFDARVXXXXXXXXXXX 182
L +PSL + A + + IIS+ TN++ S + QR+++SAF+AR+
Sbjct: 5 LCNPSLSIQSAPISVKPNKTIISI-TNSASSNNRVSTQRRTISAFEARISLIFALASQSF 63
Query: 183 XXXXRVVADLVSETSKYVVPKKFEARNLEEAFMAVPDLETVNFKVLSRKDQYEIREVEPY 362
R+VAD+ +ET+KY+ PK+FE+R LEEA M VPDLETVNFKVL+ +DQYEIRE+EPY
Sbjct: 64 SLSQRLVADVATETAKYLFPKRFESRTLEEALMTVPDLETVNFKVLTIRDQYEIREIEPY 123
Query: 363 FVAETTMPGREGFDFFGASQSFNVLAEYLFGKNIPIEKMEMTTPVI-TRKTSEGERMEMT 539
FVAETTMPG+ GFDF G+SQSFNVLAEYLFGKN EKMEMTTPV T+K S+G +M+MT
Sbjct: 124 FVAETTMPGKSGFDFRGSSQSFNVLAEYLFGKNTKKEKMEMTTPVFTTKKQSDGVKMDMT 183
Query: 540 TPVITRRVGRQEQWTMSFVMPSKYGSNLPVPKDSEVRITEVPKKIVAVAAFSGYVTDEEV 719
TPV+T + +++W MSFVMPSKYG+NLP+PKDS V I EV +K VAV +FSG+V DEEV
Sbjct: 184 TPVLTTKTVDKDEWKMSFVMPSKYGANLPLPKDSSVAIKEVARKTVAVVSFSGFVNDEEV 243
Query: 720 QRREFKLRDALKSDLEFKVKVGAEVEVLQYNPPFTLPFTRRNEIAVEVERK 872
+RRE KLR+ALK+D +FK+K G +E+ QYNPPF LPF RRNEIA+EVE K
Sbjct: 244 KRRELKLREALKNDGQFKIKEGTSIEIAQYNPPFALPFQRRNEIALEVEWK 294
>ref|XP_006339129.1| PREDICTED: heme-binding-like protein At3g10130, chloroplastic-like
isoform X2 [Solanum tuberosum]
Length = 294
Score = 339 bits (870), Expect = 1e-90
Identities = 179/273 (65%), Positives = 209/273 (76%), Gaps = 2/273 (0%)
Frame = +3
Query: 63 IISLATNNSKSMAKPQQQRQSMSAFDARVXXXXXXXXXXXXXXXRVVADLVSETSKYVVP 242
I S+A N S S P Q+R MSA +AR+ +++ +L SET+KYV P
Sbjct: 23 IKSMAVNRSSSA--PAQRRNGMSALEARISLVIALASQTSSLSQKLLTELASETAKYVFP 80
Query: 243 KK-FEARNLEEAFMAVPDLETVNFKVLSRKDQYEIREVEPYFVAETTMPGREGFDFFGAS 419
K+ FE+RNLEEA M+VPDLETV FKVL DQYEIREVEPYFVAE TMPG+ GFD GAS
Sbjct: 81 KRIFESRNLEEALMSVPDLETVKFKVLKLTDQYEIREVEPYFVAEATMPGKSGFDLNGAS 140
Query: 420 QSFNVLAEYLFGKNIPIEKMEMTTPVITRKT-SEGERMEMTTPVITRRVGRQEQWTMSFV 596
QSFN LAE+LFGKN E M MTTPVITR+T S+GE+MEMTTPVIT+R QE+W MSFV
Sbjct: 141 QSFNTLAEFLFGKNTKKESMAMTTPVITRRTQSDGEKMEMTTPVITKRAEDQEKWRMSFV 200
Query: 597 MPSKYGSNLPVPKDSEVRITEVPKKIVAVAAFSGYVTDEEVQRREFKLRDALKSDLEFKV 776
MPSKYGS+LP+PKDS V I EVP+K +AV AFSG+V DEEV+ RE +LR ALK D EF+V
Sbjct: 201 MPSKYGSDLPLPKDSSVTIKEVPRKTLAVVAFSGFVNDEEVKARESRLRTALKGDAEFQV 260
Query: 777 KVGAEVEVLQYNPPFTLPFTRRNEIAVEVERKK 875
K GA VEV QYNPPFTLPFTRRNEI++EVERK+
Sbjct: 261 KDGALVEVAQYNPPFTLPFTRRNEISLEVERKQ 293
>gb|ESW34900.1| hypothetical protein PHAVU_001G190400g [Phaseolus vulgaris]
gi|561036371|gb|ESW34901.1| hypothetical protein
PHAVU_001G190400g [Phaseolus vulgaris]
Length = 298
Score = 338 bits (868), Expect = 3e-90
Identities = 165/252 (65%), Positives = 204/252 (80%), Gaps = 1/252 (0%)
Frame = +3
Query: 114 QRQSMSAFDARVXXXXXXXXXXXXXXXRVVADLVSETSKYVVPKKFEARNLEEAFMAVPD 293
++ ++S F++R+ R++ D+ +ET+KY+ PK+FE+RNLEEA M+VPD
Sbjct: 43 RKSNISPFESRISLVFALASQANSLSQRLLTDVAAETAKYLFPKRFESRNLEEALMSVPD 102
Query: 294 LETVNFKVLSRKDQYEIREVEPYFVAETTMPGREGFDFFGASQSFNVLAEYLFGKNIPIE 473
LETV FKVLSR+DQYEIREVEPYFVAE TMPG+ GFDF GASQSFNVLAEYLFGKN E
Sbjct: 103 LETVVFKVLSRRDQYEIREVEPYFVAEATMPGKRGFDFNGASQSFNVLAEYLFGKNTKNE 162
Query: 474 KMEMTTPVITRKT-SEGERMEMTTPVITRRVGRQEQWTMSFVMPSKYGSNLPVPKDSEVR 650
KMEMTTPV T K SEG +M+MTTPV+T ++G Q++W MSFVMPSKYG+NLP+PKDS V
Sbjct: 163 KMEMTTPVFTSKNQSEGVKMDMTTPVLTTKIGDQDKWRMSFVMPSKYGANLPLPKDSSVT 222
Query: 651 ITEVPKKIVAVAAFSGYVTDEEVQRREFKLRDALKSDLEFKVKVGAEVEVLQYNPPFTLP 830
I EVP+KIVAV +FSG+V DE++++RE KLR+ALKSD +FK+K G +EV QYNPPFTLP
Sbjct: 223 IKEVPRKIVAVVSFSGFVNDEQIKQRELKLREALKSDSQFKIKEGTSIEVAQYNPPFTLP 282
Query: 831 FTRRNEIAVEVE 866
F RRNEIA+EVE
Sbjct: 283 FQRRNEIALEVE 294
>ref|XP_002882654.1| soul heme-binding family protein [Arabidopsis lyrata subsp. lyrata]
gi|297328494|gb|EFH58913.1| soul heme-binding family
protein [Arabidopsis lyrata subsp. lyrata]
Length = 317
Score = 338 bits (868), Expect = 3e-90
Identities = 170/257 (66%), Positives = 206/257 (80%), Gaps = 1/257 (0%)
Frame = +3
Query: 108 QQQRQSMSAFDARVXXXXXXXXXXXXXXXRVVADLVSETSKYVVPKKFEARNLEEAFMAV 287
Q+Q Q++SA ++RV R++ADL ET+KYV PK+F + NLEEA M+V
Sbjct: 60 QRQSQAVSATESRVSLVFALASQASSVSQRLLADLAVETAKYVFPKRFNSSNLEEALMSV 119
Query: 288 PDLETVNFKVLSRKDQYEIREVEPYFVAETTMPGREGFDFFGASQSFNVLAEYLFGKNIP 467
PDLET+ F+V+SR D+YEIR+VEPYFVAETTMPG GFDF+GAS+SFNVLAEYLFGKN
Sbjct: 120 PDLETMEFRVVSRTDKYEIRQVEPYFVAETTMPGETGFDFYGASKSFNVLAEYLFGKNTR 179
Query: 468 IEKMEMTTPVITRKT-SEGERMEMTTPVITRRVGRQEQWTMSFVMPSKYGSNLPVPKDSE 644
EKMEMTTPV+TRK S GE+MEMTTPVIT + Q QW MSFVMPSKYGSNLP+PKDS
Sbjct: 180 KEKMEMTTPVVTRKAQSVGEKMEMTTPVITTKAKDQTQWRMSFVMPSKYGSNLPLPKDSS 239
Query: 645 VRITEVPKKIVAVAAFSGYVTDEEVQRREFKLRDALKSDLEFKVKVGAEVEVLQYNPPFT 824
V+I EVP+KIVAV AFSGYVTDEE++RRE +LR AL++D +F+V+ G VEV QYNPPFT
Sbjct: 240 VKILEVPRKIVAVVAFSGYVTDEEIERREQELRRALQNDKKFRVRDGVSVEVAQYNPPFT 299
Query: 825 LPFTRRNEIAVEVERKK 875
LPF RRNE+++EVE K+
Sbjct: 300 LPFMRRNEVSLEVESKE 316
>ref|XP_006279664.1| hypothetical protein CARUB_v10026829mg [Capsella rubella]
gi|482548368|gb|EOA12562.1| hypothetical protein
CARUB_v10026829mg [Capsella rubella]
Length = 308
Score = 336 bits (862), Expect = 1e-89
Identities = 169/260 (65%), Positives = 207/260 (79%), Gaps = 1/260 (0%)
Frame = +3
Query: 99 AKPQQQRQSMSAFDARVXXXXXXXXXXXXXXXRVVADLVSETSKYVVPKKFEARNLEEAF 278
A+ +Q + ++SA ++RV R++ADL ET+KYV PK+F + NLEEA
Sbjct: 48 ARQRQSQAAVSATESRVSLVFALVSQASSVSQRLLADLAVETAKYVFPKRFNSSNLEEAL 107
Query: 279 MAVPDLETVNFKVLSRKDQYEIREVEPYFVAETTMPGREGFDFFGASQSFNVLAEYLFGK 458
M+VPDLET+NF+VLSR D+YEIREVEPYFVAETTMPG GFD +GAS+SFNVLAEYLFGK
Sbjct: 108 MSVPDLETMNFRVLSRTDKYEIREVEPYFVAETTMPGETGFDVYGASKSFNVLAEYLFGK 167
Query: 459 NIPIEKMEMTTPVITRKT-SEGERMEMTTPVITRRVGRQEQWTMSFVMPSKYGSNLPVPK 635
N EKMEMTTPV+TRK S GE+MEMTTP+IT + Q QW MSFVMPSKYGSNLP+PK
Sbjct: 168 NTIKEKMEMTTPVVTRKVESVGEKMEMTTPLITSKAKDQNQWRMSFVMPSKYGSNLPLPK 227
Query: 636 DSEVRITEVPKKIVAVAAFSGYVTDEEVQRREFKLRDALKSDLEFKVKVGAEVEVLQYNP 815
D V+I EVP+KIVAV AFSGYVT+EE++RRE +LR AL++D +F+V+ G VEV QYNP
Sbjct: 228 DPSVKIQEVPRKIVAVVAFSGYVTNEEIERREQELRRALQNDKKFRVRDGVSVEVAQYNP 287
Query: 816 PFTLPFTRRNEIAVEVERKK 875
PFTLPFTRRNE+++EVE K+
Sbjct: 288 PFTLPFTRRNEVSLEVESKE 307
>gb|EMJ03675.1| hypothetical protein PRUPE_ppa010378mg [Prunus persica]
Length = 252
Score = 336 bits (862), Expect = 1e-89
Identities = 171/251 (68%), Positives = 200/251 (79%), Gaps = 1/251 (0%)
Frame = +3
Query: 126 MSAFDARVXXXXXXXXXXXXXXXRVVADLVSETSKYVVPKKFEARNLEEAFMAVPDLETV 305
MSA +AR+ R++ +L +ET+KYV PK FEAR LEEA M+VPDLETV
Sbjct: 1 MSALEARISLVAALASQASSVSQRLLLELATETAKYVFPKNFEARTLEEALMSVPDLETV 60
Query: 306 NFKVLSRKDQYEIREVEPYFVAETTMPGREGFDFFGASQSFNVLAEYLFGKNIPIEKMEM 485
FKVLSR+DQYEIREVEPYF+AETTM G+ GFDF GASQSFNVLAEYLFGKN EKMEM
Sbjct: 61 KFKVLSRRDQYEIREVEPYFIAETTMSGKTGFDFNGASQSFNVLAEYLFGKNTTKEKMEM 120
Query: 486 TTPVITRKT-SEGERMEMTTPVITRRVGRQEQWTMSFVMPSKYGSNLPVPKDSEVRITEV 662
TTPV TRK S+G +MEMTTPVIT R+ Q++W MSFV+PSKYG+N P+PKD VRITEV
Sbjct: 121 TTPVFTRKAQSDGVKMEMTTPVITTRLEDQDKWQMSFVIPSKYGANSPLPKDPSVRITEV 180
Query: 663 PKKIVAVAAFSGYVTDEEVQRREFKLRDALKSDLEFKVKVGAEVEVLQYNPPFTLPFTRR 842
P+KIVA AFSG+VTDEEV+RRE KLR+ALK D +F+V+ G VEV QYNPPFTLPF RR
Sbjct: 181 PRKIVAAVAFSGFVTDEEVKRRESKLREALKKDGQFQVEEGTSVEVAQYNPPFTLPFQRR 240
Query: 843 NEIAVEVERKK 875
NEI++EVE K+
Sbjct: 241 NEISLEVESKE 251
>ref|XP_004494187.1| PREDICTED: heme-binding-like protein At3g10130, chloroplastic-like
isoform X1 [Cicer arietinum]
gi|502111868|ref|XP_004494188.1| PREDICTED:
heme-binding-like protein At3g10130, chloroplastic-like
isoform X2 [Cicer arietinum]
Length = 293
Score = 334 bits (857), Expect = 5e-89
Identities = 173/292 (59%), Positives = 215/292 (73%), Gaps = 7/292 (2%)
Frame = +3
Query: 18 LLSPSLKMAGAKLP------LIISLATNNSKSMAKPQQQRQSMSAFDARVXXXXXXXXXX 179
L +PSL + +P +IIS ++ + + QR+++SA +AR+
Sbjct: 5 LCTPSLSLQSPSIPKKPNNNMIISASSGD-----RLNSQRRTISASEARISLIFALASQS 59
Query: 180 XXXXXRVVADLVSETSKYVVPKKFEARNLEEAFMAVPDLETVNFKVLSRKDQYEIREVEP 359
R+ AD+ +ET+KY+ PK FE+R LEEA M VPDLETV F VLSR+DQYEIR+VEP
Sbjct: 60 TTLSQRLFADVATETAKYLFPKTFESRTLEEALMTVPDLETVKFTVLSRRDQYEIRDVEP 119
Query: 360 YFVAETTMPGREGFDFFGASQSFNVLAEYLFGKNIPIEKMEMTTPVITRKT-SEGERMEM 536
YFVAETTMPG+ GFDF GASQSFNVLAEYLFGKN EKMEMTTPV T K S+G +M+M
Sbjct: 120 YFVAETTMPGKSGFDFGGASQSFNVLAEYLFGKNTTKEKMEMTTPVFTSKNQSDGVKMDM 179
Query: 537 TTPVITRRVGRQEQWTMSFVMPSKYGSNLPVPKDSEVRITEVPKKIVAVAAFSGYVTDEE 716
TTPV+T + G Q+ W MSFVMPSKYG++LP+PKDS VRI EVP+K+VAV +FSG+V DEE
Sbjct: 180 TTPVLTTKTGDQDTWKMSFVMPSKYGASLPLPKDSSVRIEEVPRKVVAVVSFSGFVNDEE 239
Query: 717 VQRREFKLRDALKSDLEFKVKVGAEVEVLQYNPPFTLPFTRRNEIAVEVERK 872
V+RRE KLR+ALK+D + +K G VE+ QYNPPF LPF RRNEIA+EVE K
Sbjct: 240 VKRRELKLREALKNDGQLNIKEGTTVEIAQYNPPFALPFQRRNEIALEVEWK 291
>ref|XP_006407606.1| hypothetical protein EUTSA_v10021175mg [Eutrema salsugineum]
gi|557108752|gb|ESQ49059.1| hypothetical protein
EUTSA_v10021175mg [Eutrema salsugineum]
Length = 313
Score = 333 bits (853), Expect = 1e-88
Identities = 168/257 (65%), Positives = 202/257 (78%), Gaps = 1/257 (0%)
Frame = +3
Query: 108 QQQRQSMSAFDARVXXXXXXXXXXXXXXXRVVADLVSETSKYVVPKKFEARNLEEAFMAV 287
++Q + +SA +ARV R++ADL ET+KYV PK+F + NLEEAFM+V
Sbjct: 56 RRQSRHLSATEARVSLVLALASQASSASQRLLADLAVETAKYVFPKRFNSSNLEEAFMSV 115
Query: 288 PDLETVNFKVLSRKDQYEIREVEPYFVAETTMPGREGFDFFGASQSFNVLAEYLFGKNIP 467
PDLET+NF+VLSR D+YEIR+VEPY+VAETTMPG GFDF+GAS+SFNVLAEYLFGKN
Sbjct: 116 PDLETMNFRVLSRTDKYEIRQVEPYYVAETTMPGETGFDFYGASRSFNVLAEYLFGKNTV 175
Query: 468 IEKMEMTTPVITRKT-SEGERMEMTTPVITRRVGRQEQWTMSFVMPSKYGSNLPVPKDSE 644
E+MEMTTPV+TRK S GE+MEMTTPVIT + Q QW MSFVMPSKYGSNLP PKD
Sbjct: 176 NEQMEMTTPVVTRKVQSVGEKMEMTTPVITSKAKDQSQWRMSFVMPSKYGSNLPFPKDPS 235
Query: 645 VRITEVPKKIVAVAAFSGYVTDEEVQRREFKLRDALKSDLEFKVKVGAEVEVLQYNPPFT 824
V+I EVP K+VAV AFSGYV DEEV+RRE +LR AL+ D +F+ + G VEV QYNPPFT
Sbjct: 236 VKIQEVPGKVVAVVAFSGYVNDEEVERREQELRRALQYDNKFRARDGVSVEVAQYNPPFT 295
Query: 825 LPFTRRNEIAVEVERKK 875
LPFTRRNE+++EVE K+
Sbjct: 296 LPFTRRNEVSLEVESKE 312