BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Acanthopanax24_contig00017008
         (1912 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_017241874.1| PREDICTED: probable ubiquitin conjugation fa...   362   e-153
ref|XP_016561349.1| PREDICTED: probable ubiquitin conjugation fa...   358   e-152
gb|PHU27744.1| putative ubiquitin conjugation factor E4 [Capsicu...   357   e-152
ref|XP_016510685.1| PREDICTED: probable ubiquitin conjugation fa...   362   e-151
dbj|GAV65721.1| U-box domain-containing protein/Ufd2P_core domai...   359   e-151
ref|XP_019262394.1| PREDICTED: probable ubiquitin conjugation fa...   360   e-151
ref|XP_016453506.1| PREDICTED: probable ubiquitin conjugation fa...   360   e-151
ref|XP_009589734.1| PREDICTED: probable ubiquitin conjugation fa...   360   e-151
ref|XP_010551829.1| PREDICTED: probable ubiquitin conjugation fa...   357   e-150
gb|OAO94681.1| hypothetical protein AXX17_AT5G14920 [Arabidopsis...   355   e-149
ref|NP_568313.2| U-box domain-containing protein [Arabidopsis th...   355   e-149
gb|ESR44490.1| hypothetical protein CICLE_v10010958mg [Citrus cl...   353   e-148
gb|OAO94682.1| hypothetical protein AXX17_AT5G14920 [Arabidopsis...   350   e-148
gb|ABG89128.1| UFD2 [synthetic construct]                             350   e-148
ref|XP_018815848.1| PREDICTED: probable ubiquitin conjugation fa...   339   e-146
ref|XP_020243831.1| LOW QUALITY PROTEIN: probable ubiquitin conj...   330   e-138
gb|ONK60080.1| uncharacterized protein A4U43_C08F13970 [Asparagu...   330   e-138
gb|ESR44493.1| hypothetical protein CICLE_v10010958mg [Citrus cl...   353   e-131
ref|XP_012084776.1| probable ubiquitin conjugation factor E4 iso...   363   e-110
gb|PHT29598.1| putative ubiquitin conjugation factor E4 [Capsicu...   358   e-109

>ref|XP_017241874.1| PREDICTED: probable ubiquitin conjugation factor E4 [Daucus carota
            subsp. sativus]
 gb|KZN02309.1| hypothetical protein DCAR_011063 [Daucus carota subsp. sativus]
          Length = 1029

 Score =  362 bits (929), Expect(2) = e-153
 Identities = 199/343 (58%), Positives = 239/343 (69%), Gaps = 23/343 (6%)
 Frame = +2

Query: 290  LKSMQMLFKSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVV 469
            LK+MQ    SP   QL++ IARLE+ELELYSQEKLCYEA+I+ D GFI +A+SFYRLMVV
Sbjct: 500  LKAMQEQAPSP---QLNQNIARLEKELELYSQEKLCYEAQILRDGGFIQQALSFYRLMVV 556

Query: 470  WLVGLVGSFELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIM 649
            WLV LVG F++PL +TCP+                  FAS   ++LDG +  DFMNF IM
Sbjct: 557  WLVRLVGGFKMPLPSTCPREFASMPEHFVEDVMEFLIFASRIPKALDGAILDDFMNFIIM 616

Query: 650  FMASPKYIRNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDM 829
            FM SP+Y+RNPYLRAKMVEVL+ WMP  SGSS+ + TLFEG QLSL++LV+NLLKVYVD+
Sbjct: 617  FMGSPEYVRNPYLRAKMVEVLNCWMPRRSGSSATS-TLFEGHQLSLEYLVKNLLKVYVDI 675

Query: 830  EFTGNSHTQFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGS 1009
            EFTG SHTQFYDKF+IRH IAELL+YLWQ+P HR AW +IA EEEKG+YLNF NFL+N S
Sbjct: 676  EFTG-SHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEEEKGVYLNFLNFLINDS 734

Query: 1010 IYLXXXXXXXXXXXXXSDTK-----------------------DLEETIRMHMKSANEDV 1120
            IYL              + +                         E  IR+ MK ANEDV
Sbjct: 735  IYLLDESLNKILELKELEAEMSNTVEWERRPSQERQERTRLFHSQENIIRIDMKLANEDV 794

Query: 1121 NMLAYTSDQIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
             MLA+TS+QI APFLL E+VERVA MLN+FLL+LVG  P++KS
Sbjct: 795  TMLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVG--PQRKS 835



 Score =  210 bits (535), Expect(2) = e-153
 Identities = 113/154 (73%), Positives = 127/154 (82%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK LLKQIVNIYV+L RGDK +IFP+AI KDG SYNE LF+AA
Sbjct: 831  PQRKSLSLKDPEKYEFRPKHLLKQIVNIYVHLARGDKHNIFPSAITKDGRSYNEQLFSAA 890

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              +L++IGED RVIQEFVELG KAKVAA+E  DAEAALGEIP+EFLDPI Y+LM+DPVIL
Sbjct: 891  ASVLQRIGEDMRVIQEFVELGNKAKVAASEAKDAEAALGEIPDEFLDPIQYTLMQDPVIL 950

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SRITVDR VI+RHLLS  T PFN SHLT DML
Sbjct: 951  PSSRITVDRPVIQRHLLSDATDPFNRSHLTADML 984


>ref|XP_016561349.1| PREDICTED: probable ubiquitin conjugation factor E4 [Capsicum annuum]
 gb|PHT91919.1| putative ubiquitin conjugation factor E4 [Capsicum annuum]
          Length = 1039

 Score =  358 bits (918), Expect(2) = e-152
 Identities = 191/343 (55%), Positives = 237/343 (69%), Gaps = 23/343 (6%)
 Frame = +2

Query: 290  LKSMQMLFKSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVV 469
            L +M+ + +     QL + I+RLE+ELELYSQEKLCYEA+I+ D G + RA+S+YRLM+V
Sbjct: 507  LSTMKTMLEQAPSPQLQQEISRLEKELELYSQEKLCYEAQILRDGGLLQRALSYYRLMIV 566

Query: 470  WLVGLVGSFELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIM 649
            WLVGLVG F++PL + CP                +  FAS    +LDG+L  DFMNF IM
Sbjct: 567  WLVGLVGGFKMPLPSPCPMEFASMPEHFVEDAMELLIFASRIPRALDGVLLDDFMNFIIM 626

Query: 650  FMASPKYIRNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDM 829
            FMASP+YIRNPYLRAKMVEVL+ WMP  SGS++ + TLFEG QLSL++LVRNLLK+YVD+
Sbjct: 627  FMASPEYIRNPYLRAKMVEVLNCWMPRRSGSTATS-TLFEGHQLSLEYLVRNLLKLYVDI 685

Query: 830  EFTGNSHTQFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGS 1009
            EFTG SHTQFYDKF+IRH IAELL+YLWQ+P HR AW +IA EEEKG+YLNF NFL+N S
Sbjct: 686  EFTG-SHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEEEKGVYLNFLNFLINDS 744

Query: 1010 IYLXXXXXXXXXXXXXSDTK-----------------------DLEETIRMHMKSANEDV 1120
            IYL              + +                         E  IR+ MK ANEDV
Sbjct: 745  IYLLDESLNKILELKELEAEMSNTAEWERRPAQERQERTRLFHSQENIIRIDMKLANEDV 804

Query: 1121 NMLAYTSDQIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
            ++LA+TS+QI  PFLL E+VERVA MLN+FLL+LVG  P++KS
Sbjct: 805  SLLAFTSEQITVPFLLPEMVERVASMLNYFLLQLVG--PQRKS 845



 Score =  211 bits (536), Expect(2) = e-152
 Identities = 111/154 (72%), Positives = 128/154 (83%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK LLKQIV IYV+L RGDK  IFPAAI +DG SYNE +F+AA
Sbjct: 841  PQRKSLSLKDPEKYEFRPKELLKQIVKIYVHLARGDKEKIFPAAIIRDGRSYNEQIFSAA 900

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              +LR+IGED R+IQEF+ELG KAKVAA+E +DAEAALG+IP+EFLDPI Y+LMKDPVIL
Sbjct: 901  VDVLRRIGEDMRIIQEFIELGAKAKVAASEAMDAEAALGDIPDEFLDPIQYTLMKDPVIL 960

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SRITVDR VI+RHLLS +T PFN SHLTVDML
Sbjct: 961  PSSRITVDRPVIQRHLLSDSTDPFNRSHLTVDML 994


>gb|PHU27744.1| putative ubiquitin conjugation factor E4 [Capsicum chinense]
          Length = 1039

 Score =  357 bits (917), Expect(2) = e-152
 Identities = 191/343 (55%), Positives = 237/343 (69%), Gaps = 23/343 (6%)
 Frame = +2

Query: 290  LKSMQMLFKSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVV 469
            L +M+ + +     QL + I+RLE+ELELYSQEKLCYEA+I+ D G + RA+S+YRLM+V
Sbjct: 507  LSTMKTMLEQAPSPQLQQEISRLEKELELYSQEKLCYEAQILRDGGLLQRALSYYRLMIV 566

Query: 470  WLVGLVGSFELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIM 649
            WLVGLVG F++PL + CP                +  FAS    +LDG+L  DFMNF IM
Sbjct: 567  WLVGLVGGFKMPLPSPCPMEFASMPEHFVEDAMELLIFASRIPRALDGVLLDDFMNFIIM 626

Query: 650  FMASPKYIRNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDM 829
            FMASP+YIRNPYLRAKMVEVL+ WMP  SGS++ + TLFEG QLSL++LVRNLLK+YVD+
Sbjct: 627  FMASPEYIRNPYLRAKMVEVLNCWMPRRSGSTATS-TLFEGHQLSLEYLVRNLLKLYVDI 685

Query: 830  EFTGNSHTQFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGS 1009
            EFTG SHTQFYDKF+IRH IAELL+YLWQ+P HR AW +IA EEEKG+YLNF NFL+N S
Sbjct: 686  EFTG-SHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEEEKGVYLNFLNFLINDS 744

Query: 1010 IYLXXXXXXXXXXXXXSDTK-----------------------DLEETIRMHMKSANEDV 1120
            IYL              + +                         E  IR+ MK ANEDV
Sbjct: 745  IYLLDESLNKILELKELEAEMSNTAEWERRPAQERQERTRLFHTQENIIRIDMKLANEDV 804

Query: 1121 NMLAYTSDQIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
            ++LA+TS+QI  PFLL E+VERVA MLN+FLL+LVG  P++KS
Sbjct: 805  SLLAFTSEQITVPFLLPEMVERVASMLNYFLLQLVG--PQRKS 845



 Score =  211 bits (536), Expect(2) = e-152
 Identities = 111/154 (72%), Positives = 128/154 (83%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK LLKQIV IYV+L RGDK  IFPAAI +DG SYNE +F+AA
Sbjct: 841  PQRKSLSLKDPEKYEFRPKELLKQIVKIYVHLARGDKEKIFPAAIIRDGRSYNEQIFSAA 900

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              +LR+IGED R+IQEF+ELG KAKVAA+E +DAEAALG+IP+EFLDPI Y+LMKDPVIL
Sbjct: 901  VDVLRRIGEDMRIIQEFIELGAKAKVAASEAMDAEAALGDIPDEFLDPIQYTLMKDPVIL 960

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SRITVDR VI+RHLLS +T PFN SHLTVDML
Sbjct: 961  PSSRITVDRPVIQRHLLSDSTDPFNRSHLTVDML 994


>ref|XP_016510685.1| PREDICTED: probable ubiquitin conjugation factor E4 [Nicotiana
            tabacum]
          Length = 1039

 Score =  362 bits (928), Expect(2) = e-151
 Identities = 194/343 (56%), Positives = 238/343 (69%), Gaps = 23/343 (6%)
 Frame = +2

Query: 290  LKSMQMLFKSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVV 469
            L +M+ + +     QL + I+RLE+ELELYSQEKLCYEA+I+ D G + RA+SFYRLMV+
Sbjct: 507  LSTMKTMLEQAPSPQLQQEISRLEKELELYSQEKLCYEAQILRDGGLLQRALSFYRLMVI 566

Query: 470  WLVGLVGSFELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIM 649
            WLVGLVG F++PL + CP                +  FAS    +LDG+L  DFMNF IM
Sbjct: 567  WLVGLVGGFKMPLPSPCPMEFASMPEHFVEDAMELLIFASRIPRALDGVLLDDFMNFIIM 626

Query: 650  FMASPKYIRNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDM 829
            FMASP+YIRNPYLRAKMVEVL+ WMP  SGS++ + TLFEG QLSL++LVRNLLKVYVD+
Sbjct: 627  FMASPEYIRNPYLRAKMVEVLNCWMPRRSGSTATS-TLFEGHQLSLEYLVRNLLKVYVDI 685

Query: 830  EFTGNSHTQFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGS 1009
            EFTG SHTQFYDKF+IRH IAELL+YLWQ+P HR AW +IA EEEKG+YLNF NFL+N S
Sbjct: 686  EFTG-SHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEEEKGVYLNFLNFLINDS 744

Query: 1010 IYLXXXXXXXXXXXXXSDTK-----------------------DLEETIRMHMKSANEDV 1120
            IYL              + +                         E  IR+ MK ANEDV
Sbjct: 745  IYLLDESLNKILELKELEAEMSNTAEWERRPAQERQERTRLFHSQENIIRIDMKLANEDV 804

Query: 1121 NMLAYTSDQIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
            ++LA+TS+QI APFLL E+VERVA MLN+FLL+LVG  P++KS
Sbjct: 805  SLLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVG--PQRKS 845



 Score =  205 bits (521), Expect(2) = e-151
 Identities = 106/154 (68%), Positives = 128/154 (83%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK LLKQIV IYV+L RGDK +IFPAAI +DG SY++ +F+AA
Sbjct: 841  PQRKSLSLKDPEKYEFRPKELLKQIVKIYVHLARGDKENIFPAAITRDGRSYSDQIFSAA 900

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              +LR+IGED R+IQEF++LG KAKVAA+E +DAEAALG+IP+EFLDPI Y+LMKDPVIL
Sbjct: 901  ADVLRRIGEDMRIIQEFIDLGAKAKVAASEAMDAEAALGDIPDEFLDPIQYTLMKDPVIL 960

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SRITVDR VI+RHLLS ++ PFN SHLT DML
Sbjct: 961  PSSRITVDRPVIQRHLLSDSSDPFNRSHLTADML 994


>dbj|GAV65721.1| U-box domain-containing protein/Ufd2P_core domain-containing protein
            [Cephalotus follicularis]
          Length = 1027

 Score =  359 bits (922), Expect(2) = e-151
 Identities = 201/343 (58%), Positives = 239/343 (69%), Gaps = 23/343 (6%)
 Frame = +2

Query: 290  LKSMQMLFKSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVV 469
            LKSMQ     P   QL   IARLE+ +ELYSQEKLCYEA+I+ D   I RA+SFYRLMV+
Sbjct: 498  LKSMQGQAPGP---QLALDIARLEKAIELYSQEKLCYEAQILRDGSLIQRALSFYRLMVI 554

Query: 470  WLVGLVGSFELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIM 649
            WLVGLVG F++PL +TCP                +  FAS   ++LDG+L  DFMNF IM
Sbjct: 555  WLVGLVGGFKMPLPSTCPMEFASMPEHFVEDAMELLIFASRIPKALDGVLLDDFMNFIIM 614

Query: 650  FMASPKYIRNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDM 829
            FMASP+YIRNPYLRAKMVEVL+ WMP  SGSS+ A TLFEG QLSL++LVRNLLK+YVD+
Sbjct: 615  FMASPEYIRNPYLRAKMVEVLNCWMPRRSGSSATA-TLFEGHQLSLEYLVRNLLKLYVDI 673

Query: 830  EFTGNSHTQFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGS 1009
            EFTG SHTQFYDKF+IRH IAELL+YLWQ+P HR  W +IA EEEKG+YLNF NFL+N S
Sbjct: 674  EFTG-SHTQFYDKFNIRHNIAELLEYLWQVPSHRNTWRQIAKEEEKGVYLNFLNFLINDS 732

Query: 1010 IY-----LXXXXXXXXXXXXXSDTKDLEE------------------TIRMHMKSANEDV 1120
            IY     L             S+T + E+                   IR+ MK ANEDV
Sbjct: 733  IYLLDESLNKILELKELEAEMSNTAEWEQRPAQERQDRTRLFHSQENIIRIDMKLANEDV 792

Query: 1121 NMLAYTSDQIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
            +MLA+TS+QI  PFLLSE+VERVA MLN+FLL+LVG  P++KS
Sbjct: 793  SMLAFTSEQITVPFLLSEMVERVASMLNYFLLQLVG--PQRKS 833



 Score =  206 bits (524), Expect(2) = e-151
 Identities = 109/154 (70%), Positives = 127/154 (82%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK+LLKQIV IYV+L RGD  +IFPAAI++DG SYNE LF AA
Sbjct: 829  PQRKSLSLKDPEKYEFRPKQLLKQIVCIYVHLARGDSENIFPAAISRDGRSYNEQLFTAA 888

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              +L +IGED R+IQEF+ELG KAKVAA+E +DAEAALG+IP+EFLDPI Y+LMKDPVIL
Sbjct: 889  AEVLWRIGEDGRIIQEFIELGSKAKVAASEAMDAEAALGDIPDEFLDPIQYTLMKDPVIL 948

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SRITVDR VI+RHLLS N+ PFN SHLT DML
Sbjct: 949  PSSRITVDRPVIQRHLLSDNSDPFNRSHLTADML 982


>ref|XP_019262394.1| PREDICTED: probable ubiquitin conjugation factor E4 [Nicotiana
            attenuata]
 gb|OIT37848.1| putative ubiquitin conjugation factor e4 [Nicotiana attenuata]
          Length = 1040

 Score =  360 bits (924), Expect(2) = e-151
 Identities = 193/343 (56%), Positives = 238/343 (69%), Gaps = 23/343 (6%)
 Frame = +2

Query: 290  LKSMQMLFKSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVV 469
            L +M+ + +     QL + I+RLE+ELELYSQEKLCYEA+I+ D G + RA+SFYRLMV+
Sbjct: 508  LSTMKTMLEQAPSPQLQQEISRLEKELELYSQEKLCYEAQILRDGGLLQRALSFYRLMVI 567

Query: 470  WLVGLVGSFELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIM 649
            WLVGLVG F++PL + CP                +  FAS    +LDG+L  DFMNF IM
Sbjct: 568  WLVGLVGGFKMPLPSPCPMQFASMPEHFVEDTMELLIFASRIPRALDGVLLDDFMNFIIM 627

Query: 650  FMASPKYIRNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDM 829
            FMASP+YIRNPYLRAKMVEVL+ WMP  SGS++ + TLFEG QLSL++LV+NLLKVYVD+
Sbjct: 628  FMASPEYIRNPYLRAKMVEVLNCWMPRRSGSTATS-TLFEGHQLSLEYLVKNLLKVYVDI 686

Query: 830  EFTGNSHTQFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGS 1009
            EFTG SHTQFYDKF+IRH IAELL+YLWQ+P HR AW +IA EEEKG+YLNF NFL+N S
Sbjct: 687  EFTG-SHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEEEKGVYLNFLNFLINDS 745

Query: 1010 IYLXXXXXXXXXXXXXSDTK-----------------------DLEETIRMHMKSANEDV 1120
            IYL              + +                         E  IR+ MK ANEDV
Sbjct: 746  IYLLDESLNKILELKELEAEMSNTAEWERRPAQERQERTRLFHSQENIIRIDMKLANEDV 805

Query: 1121 NMLAYTSDQIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
            ++LA+TS+QI APFLL E+VERVA MLN+FLL+LVG  P++KS
Sbjct: 806  SLLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVG--PQRKS 846



 Score =  205 bits (521), Expect(2) = e-151
 Identities = 106/154 (68%), Positives = 128/154 (83%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK LLKQIV IYV+L RGDK +IFPAAI +DG SY++ +F+AA
Sbjct: 842  PQRKSLSLKDPEKYEFRPKELLKQIVKIYVHLARGDKENIFPAAITRDGRSYSDQIFSAA 901

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              +LR+IGED R+IQEF++LG KAKVAA+E +DAEAALG+IP+EFLDPI Y+LMKDPVIL
Sbjct: 902  ADVLRRIGEDMRIIQEFIDLGAKAKVAASEAMDAEAALGDIPDEFLDPIQYTLMKDPVIL 961

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SRITVDR VI+RHLLS ++ PFN SHLT DML
Sbjct: 962  PSSRITVDRPVIQRHLLSDSSDPFNRSHLTADML 995


>ref|XP_016453506.1| PREDICTED: probable ubiquitin conjugation factor E4 [Nicotiana
            tabacum]
          Length = 1040

 Score =  360 bits (924), Expect(2) = e-151
 Identities = 193/343 (56%), Positives = 238/343 (69%), Gaps = 23/343 (6%)
 Frame = +2

Query: 290  LKSMQMLFKSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVV 469
            L +M+ + +     QL + ++RLE+ELELYSQEKLCYEA+I+ D G + RA+SFYRLMVV
Sbjct: 508  LSTMKTMLEQAPSPQLQQELSRLEKELELYSQEKLCYEAQILRDGGLLQRALSFYRLMVV 567

Query: 470  WLVGLVGSFELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIM 649
            WLVGLVG F++PL + CP                +  FAS    +LDG+L  DFMNF IM
Sbjct: 568  WLVGLVGGFKMPLPSPCPMEFASMPEHFVEDAMELLIFASRIPRALDGVLLDDFMNFIIM 627

Query: 650  FMASPKYIRNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDM 829
            FMASP+YIRNPYLRAKMVEVL+ WMP  SGS++ + TLFEG QLSL++LV+NLLKVYVD+
Sbjct: 628  FMASPEYIRNPYLRAKMVEVLNCWMPRRSGSTATS-TLFEGHQLSLEYLVKNLLKVYVDI 686

Query: 830  EFTGNSHTQFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGS 1009
            EFTG SHTQFYDKF+IRH IAELL+YLWQ+P HR AW +IA EEEKG+YLNF NFL+N S
Sbjct: 687  EFTG-SHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEEEKGVYLNFLNFLINDS 745

Query: 1010 IYLXXXXXXXXXXXXXSDTK-----------------------DLEETIRMHMKSANEDV 1120
            IYL              + +                         E  IR+ MK ANEDV
Sbjct: 746  IYLLDESLNKILELKELEAEMSNTAEWERRPAQERQERTRLFHSQENIIRIDMKLANEDV 805

Query: 1121 NMLAYTSDQIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
            ++LA+TS+QI APFLL E+VERVA MLN+FLL+LVG  P++KS
Sbjct: 806  SLLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVG--PQRKS 846



 Score =  205 bits (521), Expect(2) = e-151
 Identities = 106/154 (68%), Positives = 128/154 (83%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK LLKQIV IYV+L RGDK +IFPAAI +DG SY++ +F+AA
Sbjct: 842  PQRKSLSLKDPEKYEFRPKELLKQIVKIYVHLARGDKENIFPAAITRDGRSYSDQIFSAA 901

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              +LR+IGED R+IQEF++LG KAKVAA+E +DAEAALG+IP+EFLDPI Y+LMKDPVIL
Sbjct: 902  ADVLRRIGEDMRIIQEFIDLGAKAKVAASEAMDAEAALGDIPDEFLDPIQYTLMKDPVIL 961

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SRITVDR VI+RHLLS ++ PFN SHLT DML
Sbjct: 962  PSSRITVDRPVIQRHLLSDSSDPFNRSHLTADML 995


>ref|XP_009589734.1| PREDICTED: probable ubiquitin conjugation factor E4 [Nicotiana
            tomentosiformis]
          Length = 1040

 Score =  360 bits (924), Expect(2) = e-151
 Identities = 193/343 (56%), Positives = 238/343 (69%), Gaps = 23/343 (6%)
 Frame = +2

Query: 290  LKSMQMLFKSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVV 469
            L +M+ + +     QL + ++RLE+ELELYSQEKLCYEA+I+ D G + RA+SFYRLMVV
Sbjct: 508  LSTMKTMLEQAPSPQLQQELSRLEKELELYSQEKLCYEAQILRDGGLLQRALSFYRLMVV 567

Query: 470  WLVGLVGSFELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIM 649
            WLVGLVG F++PL + CP                +  FAS    +LDG+L  DFMNF IM
Sbjct: 568  WLVGLVGGFKMPLPSPCPMEFASMPEHFVEDAMELLIFASRIPRALDGVLLDDFMNFIIM 627

Query: 650  FMASPKYIRNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDM 829
            FMASP+YIRNPYLRAKMVEVL+ WMP  SGS++ + TLFEG QLSL++LV+NLLKVYVD+
Sbjct: 628  FMASPEYIRNPYLRAKMVEVLNCWMPRRSGSTATS-TLFEGHQLSLEYLVKNLLKVYVDI 686

Query: 830  EFTGNSHTQFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGS 1009
            EFTG SHTQFYDKF+IRH IAELL+YLWQ+P HR AW +IA EEEKG+YLNF NFL+N S
Sbjct: 687  EFTG-SHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEEEKGVYLNFLNFLINDS 745

Query: 1010 IYLXXXXXXXXXXXXXSDTK-----------------------DLEETIRMHMKSANEDV 1120
            IYL              + +                         E  IR+ MK ANEDV
Sbjct: 746  IYLLDESLNKILELKELEAEMSNTAEWERRPAQERQERTRLFHSQENIIRIDMKLANEDV 805

Query: 1121 NMLAYTSDQIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
            ++LA+TS+QI APFLL E+VERVA MLN+FLL+LVG  P++KS
Sbjct: 806  SLLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVG--PQRKS 846



 Score =  205 bits (521), Expect(2) = e-151
 Identities = 106/154 (68%), Positives = 128/154 (83%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK LLKQIV IYV+L RGDK +IFPAAI +DG SY++ +F+AA
Sbjct: 842  PQRKSLSLKDPEKYEFRPKELLKQIVKIYVHLARGDKENIFPAAITRDGRSYSDQIFSAA 901

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              +LR+IGED R+IQEF++LG KAKVAA+E +DAEAALG+IP+EFLDPI Y+LMKDPVIL
Sbjct: 902  ADVLRRIGEDMRIIQEFIDLGAKAKVAASEAMDAEAALGDIPDEFLDPIQYTLMKDPVIL 961

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SRITVDR VI+RHLLS ++ PFN SHLT DML
Sbjct: 962  PSSRITVDRPVIQRHLLSDSSDPFNRSHLTADML 995


>ref|XP_010551829.1| PREDICTED: probable ubiquitin conjugation factor E4 [Tarenaya
            hassleriana]
          Length = 1038

 Score =  357 bits (917), Expect(2) = e-150
 Identities = 196/343 (57%), Positives = 239/343 (69%), Gaps = 23/343 (6%)
 Frame = +2

Query: 290  LKSMQMLFKSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVV 469
            LK M++   SP   QL   IARLE+E+ELY QEKLCYEA+I+ D  FI RA+SFYRL+VV
Sbjct: 510  LKGMEVQAPSP---QLKLDIARLEKEIELYLQEKLCYEAQILRDGDFIQRALSFYRLVVV 566

Query: 470  WLVGLVGSFELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIM 649
            WLVGLVG F++PL +TCP                +  FAS   ++LDG++  DFMNF IM
Sbjct: 567  WLVGLVGGFKMPLPSTCPMEFACMPEHFVEDAMELLIFASRIPKALDGVILDDFMNFIIM 626

Query: 650  FMASPKYIRNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDM 829
            FMASP+YIRNPYLRAKMVEVL+ WMP  SGS++ + TLFEG QLSL++LVRNLLK+YVD+
Sbjct: 627  FMASPEYIRNPYLRAKMVEVLNCWMPRTSGSTATS-TLFEGHQLSLEYLVRNLLKLYVDI 685

Query: 830  EFTGNSHTQFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGS 1009
            EFTG SHTQFYDKF+IRH IAELL+YLWQ+P HR AW +IA EEEKG+YLNF NFL+N S
Sbjct: 686  EFTG-SHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEEEKGVYLNFLNFLINDS 744

Query: 1010 IYLXXXXXXXXXXXXXSDTK-----------------------DLEETIRMHMKSANEDV 1120
            IYL              + +                         E  IR+ MK ANEDV
Sbjct: 745  IYLLDESLNKILELKQLEAEMANTAEWEQRPAQERQERTRLFHSQENIIRIDMKLANEDV 804

Query: 1121 NMLAYTSDQIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
            ++LA+TS+QI APFLL E+VERVA MLN+FLL+LVG  P++KS
Sbjct: 805  SILAFTSEQITAPFLLPEMVERVANMLNYFLLQLVG--PQRKS 845



 Score =  204 bits (519), Expect(2) = e-150
 Identities = 107/154 (69%), Positives = 125/154 (81%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK+LLKQIV IYV+L RGD  +IFP AI+ DG SYNE LF+AA
Sbjct: 841  PQRKSLTLKDPEKYEFRPKQLLKQIVRIYVHLARGDSENIFPTAISSDGRSYNEKLFSAA 900

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              +LRKIGED ++IQEF+ELG KAK AA+E +DAEAALGEIP+EFLDPI Y+LM+DPVIL
Sbjct: 901  ADVLRKIGEDGKIIQEFIELGAKAKAAASEAMDAEAALGEIPDEFLDPIQYTLMRDPVIL 960

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SRITVDR VI+RHLLS N  PFN +HLT DML
Sbjct: 961  PSSRITVDRPVIQRHLLSDNHDPFNRAHLTADML 994


>gb|OAO94681.1| hypothetical protein AXX17_AT5G14920 [Arabidopsis thaliana]
          Length = 1038

 Score =  355 bits (911), Expect(2) = e-149
 Identities = 196/343 (57%), Positives = 239/343 (69%), Gaps = 23/343 (6%)
 Frame = +2

Query: 290  LKSMQMLFKSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVV 469
            LK+M+    SP   QL+  I+R+E+ELEL SQEKLC+EA+I+ D  FI RA+SFYRLMVV
Sbjct: 509  LKAMRDQAPSP---QLELDISRMEKELELSSQEKLCHEAQILRDGDFIQRALSFYRLMVV 565

Query: 470  WLVGLVGSFELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIM 649
            WLVGLVG F++PL +TCP                +  FAS   ++LDG+   DFMNF IM
Sbjct: 566  WLVGLVGGFKMPLPSTCPMEFSCMPEHFVEDAMELLIFASRIPKALDGVPLDDFMNFIIM 625

Query: 650  FMASPKYIRNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDM 829
            FMASP+Y+RNPYLRAKMVEVL+ WMP  S SSS   TLFEG QLSL++LVRNLLK+YVD+
Sbjct: 626  FMASPEYVRNPYLRAKMVEVLNCWMPRSSSSSSATSTLFEGHQLSLEYLVRNLLKLYVDI 685

Query: 830  EFTGNSHTQFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGS 1009
            EFTG SHTQFYDKF+IRH IAELL+YLWQ+P HR AW +IA +EEKG+YLNF NFLVN S
Sbjct: 686  EFTG-SHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRRIAKDEEKGVYLNFLNFLVNDS 744

Query: 1010 IY-----LXXXXXXXXXXXXXSDTKDLEE------------------TIRMHMKSANEDV 1120
            IY     L             S+T + E+                   +R+ MK ANEDV
Sbjct: 745  IYLLDESLNKILEIKQIEADMSNTAEWEQRPTQERQERTRLFHSQENIVRIDMKLANEDV 804

Query: 1121 NMLAYTSDQIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
             MLA+TS++I APFLL E+VERVA MLN+FLL+LVG  P++KS
Sbjct: 805  TMLAFTSEEITAPFLLPEMVERVANMLNYFLLQLVG--PQRKS 845



 Score =  204 bits (519), Expect(2) = e-149
 Identities = 107/154 (69%), Positives = 124/154 (80%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK+LLKQIV IYVNL RGD  +IFP AI+ DG SYNE LFNA 
Sbjct: 841  PQRKSLSLKDPEKYEFRPKQLLKQIVRIYVNLARGDTVNIFPGAISSDGRSYNEQLFNAG 900

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              +LR+IGE+ R+IQEF+ELG KAK AA+E LDAEAALGEIP+EFLDPI Y+LM+DPVIL
Sbjct: 901  ADVLRRIGEEGRIIQEFMELGTKAKAAASEALDAEAALGEIPDEFLDPIQYTLMRDPVIL 960

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SRITVDR +I+RHLLS N  PFN +HLT DML
Sbjct: 961  PSSRITVDRPIIQRHLLSDNHDPFNRAHLTSDML 994


>ref|NP_568313.2| U-box domain-containing protein [Arabidopsis thaliana]
 sp|Q9LF41.1|UBE4_ARATH RecName: Full=Probable ubiquitin conjugation factor E4; AltName:
            Full=Plant U-box protein 1; AltName: Full=RING-type E3
            ubiquitin transferase E4; AltName: Full=U-box
            domain-containing protein 1; AltName:
            Full=Ubiquitin-fusion degradation protein 2-like;
            Short=UB fusion protein 2-like
 emb|CAC01739.1| ubiquitin-fusion degradation protein-like [Arabidopsis thaliana]
 gb|AED92156.1| U-box domain-containing protein [Arabidopsis thaliana]
          Length = 1038

 Score =  355 bits (911), Expect(2) = e-149
 Identities = 196/343 (57%), Positives = 239/343 (69%), Gaps = 23/343 (6%)
 Frame = +2

Query: 290  LKSMQMLFKSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVV 469
            LK+M+    SP   QL+  I+R+E+ELEL SQEKLC+EA+I+ D  FI RA+SFYRLMVV
Sbjct: 509  LKAMRDQAPSP---QLELDISRMEKELELSSQEKLCHEAQILRDGDFIQRALSFYRLMVV 565

Query: 470  WLVGLVGSFELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIM 649
            WLVGLVG F++PL +TCP                +  FAS   ++LDG+   DFMNF IM
Sbjct: 566  WLVGLVGGFKMPLPSTCPMEFSCMPEHFVEDAMELLIFASRIPKALDGVPLDDFMNFIIM 625

Query: 650  FMASPKYIRNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDM 829
            FMASP+Y+RNPYLRAKMVEVL+ WMP  S SSS   TLFEG QLSL++LVRNLLK+YVD+
Sbjct: 626  FMASPEYVRNPYLRAKMVEVLNCWMPRSSSSSSATSTLFEGHQLSLEYLVRNLLKLYVDI 685

Query: 830  EFTGNSHTQFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGS 1009
            EFTG SHTQFYDKF+IRH IAELL+YLWQ+P HR AW +IA +EEKG+YLNF NFLVN S
Sbjct: 686  EFTG-SHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRRIAKDEEKGVYLNFLNFLVNDS 744

Query: 1010 IY-----LXXXXXXXXXXXXXSDTKDLEE------------------TIRMHMKSANEDV 1120
            IY     L             S+T + E+                   +R+ MK ANEDV
Sbjct: 745  IYLLDESLNKILEIKQIEADMSNTAEWEQRPTQERQERTRLFHSQENIVRIDMKLANEDV 804

Query: 1121 NMLAYTSDQIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
             MLA+TS++I APFLL E+VERVA MLN+FLL+LVG  P++KS
Sbjct: 805  TMLAFTSEEITAPFLLPEMVERVANMLNYFLLQLVG--PQRKS 845



 Score =  204 bits (519), Expect(2) = e-149
 Identities = 107/154 (69%), Positives = 124/154 (80%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK+LLKQIV IYVNL RGD  +IFP AI+ DG SYNE LFNA 
Sbjct: 841  PQRKSLSLKDPEKYEFRPKQLLKQIVRIYVNLARGDTVNIFPGAISSDGRSYNEQLFNAG 900

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              +LR+IGE+ R+IQEF+ELG KAK AA+E LDAEAALGEIP+EFLDPI Y+LM+DPVIL
Sbjct: 901  ADVLRRIGEEGRIIQEFMELGTKAKAAASEALDAEAALGEIPDEFLDPIQYTLMRDPVIL 960

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SRITVDR +I+RHLLS N  PFN +HLT DML
Sbjct: 961  PSSRITVDRPIIQRHLLSDNHDPFNRAHLTSDML 994


>gb|ESR44490.1| hypothetical protein CICLE_v10010958mg [Citrus clementina]
 gb|ESR44491.1| hypothetical protein CICLE_v10010958mg [Citrus clementina]
          Length = 564

 Score =  353 bits (905), Expect(2) = e-148
 Identities = 190/335 (56%), Positives = 232/335 (69%), Gaps = 23/335 (6%)
 Frame = +2

Query: 314  KSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVVWLVGLVGS 493
            ++PS +QL+  I R+E+E+EL SQEKLCYEA+I+ D   I  A+SFYRLM+VWLV LVG 
Sbjct: 40   QTPS-SQLNLEITRIEKEIELSSQEKLCYEAQILRDGDLIQHALSFYRLMIVWLVDLVGG 98

Query: 494  FELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIMFMASPKYI 673
            F++PL  TCP                +  FAS   ++LDG+L  DFMNF IMFMASPKYI
Sbjct: 99   FKMPLPDTCPMEFACMPEHFVEDAMELLIFASRIPKALDGVLLDDFMNFIIMFMASPKYI 158

Query: 674  RNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDMEFTGNSHT 853
            RNPYLR+KMVEVL+ WMP  SGSSS   TLFEG Q+SL++LVRNLLK+YVD+EFTG SHT
Sbjct: 159  RNPYLRSKMVEVLNCWMPRRSGSSSATATLFEGHQMSLEYLVRNLLKLYVDIEFTG-SHT 217

Query: 854  QFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGSIYLXXXXX 1033
            QFYDKF+IRH IAELL+YLWQ+P HR AW +IA EEEKG+YLNF NFL+N SIYL     
Sbjct: 218  QFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEEEKGVYLNFLNFLINDSIYLLDESL 277

Query: 1034 XXXXXXXXSDTK-----------------------DLEETIRMHMKSANEDVNMLAYTSD 1144
                     + +                         E  IR+ MK ANEDV+MLA+TS+
Sbjct: 278  NKILELKVIEAEMSNTAEWERRPAQERQERTRLFHSQENIIRIDMKLANEDVSMLAFTSE 337

Query: 1145 QIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
            QI APFLL E++ERVA MLN+FLL+LVG  P++KS
Sbjct: 338  QIVAPFLLPEMIERVASMLNYFLLQLVG--PQRKS 370



 Score =  202 bits (514), Expect(2) = e-148
 Identities = 108/154 (70%), Positives = 125/154 (81%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK+LLKQIV IYV+L RGD  ++FPAAI+ DG SYNE LF+AA
Sbjct: 366  PQRKSLTLKDPEKYEFRPKQLLKQIVCIYVHLARGDTQNLFPAAISSDGRSYNEQLFSAA 425

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              +L KIGED R+IQEF+ELG KAK AA+E +DAEAALG+IP+EFLDPI Y+LMKDPVIL
Sbjct: 426  ADVLWKIGEDGRIIQEFIELGAKAKAAASEAMDAEAALGDIPDEFLDPIQYTLMKDPVIL 485

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SRITVDR VI+RHLLS  T PFN SHLT DML
Sbjct: 486  PSSRITVDRPVIQRHLLSDATDPFNRSHLTADML 519


>gb|OAO94682.1| hypothetical protein AXX17_AT5G14920 [Arabidopsis thaliana]
          Length = 1037

 Score =  350 bits (899), Expect(2) = e-148
 Identities = 195/343 (56%), Positives = 240/343 (69%), Gaps = 23/343 (6%)
 Frame = +2

Query: 290  LKSMQMLFKSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVV 469
            LK+M+    SP   QL+  I+R+E+ELEL SQEKLC+EA+I+ D  FI RA+SFYRLMVV
Sbjct: 509  LKAMRDQAPSP---QLELDISRMEKELELSSQEKLCHEAQILRDGDFIQRALSFYRLMVV 565

Query: 470  WLVGLVGSFELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIM 649
            WLVGLVG F++PL +TCP                +  FAS   ++LDG+   DFMNF IM
Sbjct: 566  WLVGLVGGFKMPLPSTCPMEFSCMPEHFVEDAMELLIFASRIPKALDGVPLDDFMNFIIM 625

Query: 650  FMASPKYIRNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDM 829
            FMASP+Y+RNPYLRAKMVEVL+ WMP  S SS+ + TLFEG QLSL++LVRNLLK+YVD+
Sbjct: 626  FMASPEYVRNPYLRAKMVEVLNCWMPRSSSSSATS-TLFEGHQLSLEYLVRNLLKLYVDI 684

Query: 830  EFTGNSHTQFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGS 1009
            EFTG SHTQFYDKF+IRH IAELL+YLWQ+P HR AW +IA +EEKG+YLNF NFLVN S
Sbjct: 685  EFTG-SHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRRIAKDEEKGVYLNFLNFLVNDS 743

Query: 1010 IY-----LXXXXXXXXXXXXXSDTKDLEE------------------TIRMHMKSANEDV 1120
            IY     L             S+T + E+                   +R+ MK ANEDV
Sbjct: 744  IYLLDESLNKILEIKQIEADMSNTAEWEQRPTQERQERTRLFHSQENIVRIDMKLANEDV 803

Query: 1121 NMLAYTSDQIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
             MLA+TS++I APFLL E+VERVA MLN+FLL+LVG  P++KS
Sbjct: 804  TMLAFTSEEITAPFLLPEMVERVANMLNYFLLQLVG--PQRKS 844



 Score =  204 bits (519), Expect(2) = e-148
 Identities = 107/154 (69%), Positives = 124/154 (80%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK+LLKQIV IYVNL RGD  +IFP AI+ DG SYNE LFNA 
Sbjct: 840  PQRKSLSLKDPEKYEFRPKQLLKQIVRIYVNLARGDTVNIFPGAISSDGRSYNEQLFNAG 899

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              +LR+IGE+ R+IQEF+ELG KAK AA+E LDAEAALGEIP+EFLDPI Y+LM+DPVIL
Sbjct: 900  ADVLRRIGEEGRIIQEFMELGTKAKAAASEALDAEAALGEIPDEFLDPIQYTLMRDPVIL 959

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SRITVDR +I+RHLLS N  PFN +HLT DML
Sbjct: 960  PSSRITVDRPIIQRHLLSDNHDPFNRAHLTSDML 993


>gb|ABG89128.1| UFD2 [synthetic construct]
          Length = 1037

 Score =  350 bits (899), Expect(2) = e-148
 Identities = 195/343 (56%), Positives = 240/343 (69%), Gaps = 23/343 (6%)
 Frame = +2

Query: 290  LKSMQMLFKSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVV 469
            LK+M+    SP   QL+  I+R+E+ELEL SQEKLC+EA+I+ D  FI RA+SFYRLMVV
Sbjct: 509  LKAMRDQAPSP---QLELDISRMEKELELSSQEKLCHEAQILRDGDFIQRALSFYRLMVV 565

Query: 470  WLVGLVGSFELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIM 649
            WLVGLVG F++PL +TCP                +  FAS   ++LDG+   DFMNF IM
Sbjct: 566  WLVGLVGGFKMPLPSTCPMEFSCMPEHFVEDAMELLIFASRIPKALDGVPLDDFMNFIIM 625

Query: 650  FMASPKYIRNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDM 829
            FMASP+Y+RNPYLRAKMVEVL+ WMP  S SS+ + TLFEG QLSL++LVRNLLK+YVD+
Sbjct: 626  FMASPEYVRNPYLRAKMVEVLNCWMPRSSSSSATS-TLFEGHQLSLEYLVRNLLKLYVDI 684

Query: 830  EFTGNSHTQFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGS 1009
            EFTG SHTQFYDKF+IRH IAELL+YLWQ+P HR AW +IA +EEKG+YLNF NFLVN S
Sbjct: 685  EFTG-SHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRRIAKDEEKGVYLNFLNFLVNDS 743

Query: 1010 IY-----LXXXXXXXXXXXXXSDTKDLEE------------------TIRMHMKSANEDV 1120
            IY     L             S+T + E+                   +R+ MK ANEDV
Sbjct: 744  IYLLDESLNKILEIKQIEADMSNTAEWEQRPTQERQERTRLFHSQENIVRIDMKLANEDV 803

Query: 1121 NMLAYTSDQIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
             MLA+TS++I APFLL E+VERVA MLN+FLL+LVG  P++KS
Sbjct: 804  TMLAFTSEEITAPFLLPEMVERVANMLNYFLLQLVG--PQRKS 844



 Score =  204 bits (519), Expect(2) = e-148
 Identities = 107/154 (69%), Positives = 124/154 (80%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK+LLKQIV IYVNL RGD  +IFP AI+ DG SYNE LFNA 
Sbjct: 840  PQRKSLSLKDPEKYEFRPKQLLKQIVRIYVNLARGDTVNIFPGAISSDGRSYNEQLFNAG 899

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              +LR+IGE+ R+IQEF+ELG KAK AA+E LDAEAALGEIP+EFLDPI Y+LM+DPVIL
Sbjct: 900  ADVLRRIGEEGRIIQEFMELGTKAKAAASEALDAEAALGEIPDEFLDPIQYTLMRDPVIL 959

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SRITVDR +I+RHLLS N  PFN +HLT DML
Sbjct: 960  PSSRITVDRPIIQRHLLSDNHDPFNRAHLTSDML 993


>ref|XP_018815848.1| PREDICTED: probable ubiquitin conjugation factor E4 [Juglans regia]
          Length = 1042

 Score =  339 bits (870), Expect(2) = e-146
 Identities = 189/343 (55%), Positives = 236/343 (68%), Gaps = 23/343 (6%)
 Frame = +2

Query: 290  LKSMQMLFKSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVV 469
            LKSMQ    +P+   L   ++RLE+E+EL SQEKLCYEA+++ D   I  A+SFYRLMVV
Sbjct: 513  LKSMQGHASTPA---LKLEVSRLEKEIELNSQEKLCYEAQMLRDGALIQNALSFYRLMVV 569

Query: 470  WLVGLVGSFELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIM 649
            WLV LVG F++PL +TCP                +  FAS   ++LDG+L  DFMNF IM
Sbjct: 570  WLVSLVGGFKMPLPSTCPMEFAAMPEHFVEDAMELLIFASRIPKALDGVLLDDFMNFIIM 629

Query: 650  FMASPKYIRNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDM 829
            FMASP++IRNPYL+AKMVEVL+ WMP  SG S V  TLFEG QLSL++LVRNLLK+YVD+
Sbjct: 630  FMASPEFIRNPYLKAKMVEVLNCWMPRGSGPS-VTATLFEGHQLSLEYLVRNLLKLYVDI 688

Query: 830  EFTGNSHTQFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGS 1009
            EFTG +H QF+DKF+IRH IAELL+YLWQ+P HR AW +IA EEEKG+YL F NFL+N S
Sbjct: 689  EFTG-AHNQFFDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEEEKGVYLTFLNFLINDS 747

Query: 1010 IY-----LXXXXXXXXXXXXXSDTKDLEE------------------TIRMHMKSANEDV 1120
            IY     L             S+T + E+                   I+++MK ANEDV
Sbjct: 748  IYLLDESLNKILELKELEAEMSNTTEWEQRPAQERQERTRLFHSQENIIQINMKLANEDV 807

Query: 1121 NMLAYTSDQIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
            +MLA+TS+QI APFLL E+VERVA MLN+FLL+LVG  P++KS
Sbjct: 808  SMLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVG--PQRKS 848



 Score =  209 bits (532), Expect(2) = e-146
 Identities = 112/154 (72%), Positives = 128/154 (83%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK+LLKQIV IYV+L RGD  +IFPAAI+KDG SYNE LF+AA
Sbjct: 844  PQRKSLTLKDPEKYEFRPKQLLKQIVYIYVHLARGDTENIFPAAISKDGRSYNEQLFSAA 903

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              +LR+IGE+ RVIQEFV+LG KAKVAA+E +D EAALGEIP+EFLDPI Y+LMKDPVIL
Sbjct: 904  ADVLRRIGENGRVIQEFVQLGAKAKVAASEAMDTEAALGEIPDEFLDPIQYTLMKDPVIL 963

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SRITVDR VI+RHLLS NT PFN SHLT DML
Sbjct: 964  PSSRITVDRPVIQRHLLSDNTDPFNRSHLTADML 997


>ref|XP_020243831.1| LOW QUALITY PROTEIN: probable ubiquitin conjugation factor E4
            [Asparagus officinalis]
          Length = 1026

 Score =  330 bits (847), Expect(2) = e-138
 Identities = 177/329 (53%), Positives = 221/329 (67%), Gaps = 23/329 (6%)
 Frame = +2

Query: 332  QLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVVWLVGLVGSFELPLS 511
            QL+E I RLE+ +E+ SQ+KLCYEA+I+ D   + RA+SFYRLMVVWLV LVG F++PL 
Sbjct: 506  QLEENIKRLEKIIEILSQQKLCYEAQILRDGALLQRALSFYRLMVVWLVSLVGGFKMPLP 565

Query: 512  TTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIMFMASPKYIRNPYLR 691
            + CP                +  F S   ++LDG +  DF++F IMFMASP Y++NPYLR
Sbjct: 566  SNCPMEFACMPEHFIEDSMDLLIFTSRIPKALDGFVLDDFLSFIIMFMASPSYVKNPYLR 625

Query: 692  AKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDMEFTGNSHTQFYDKF 871
            AKMVEVL+ WMP  SG SS+A +LFEG QLSL +LVRNLLK+YVD+EFTG SHTQFYDKF
Sbjct: 626  AKMVEVLNCWMPERSGLSSIA-SLFEGHQLSLDYLVRNLLKLYVDIEFTG-SHTQFYDKF 683

Query: 872  DIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGSIYLXXXXXXXXXXX 1051
            +IRH IA LL+YLW +P HR AW +IA +EEKG+YLNF NFL+N SI+L           
Sbjct: 684  NIRHNIAGLLEYLWNVPSHRNAWRQIAIKEEKGVYLNFLNFLINDSIFLLDESLNIILEL 743

Query: 1052 XXSDTK-----------------------DLEETIRMHMKSANEDVNMLAYTSDQIAAPF 1162
               + +                         E  +R  MK ANEDV MLA+TS+QI APF
Sbjct: 744  KGIEAEMSNSAEWERRPAQEREERLRLFHSRENIVRFDMKLANEDVGMLAFTSEQIPAPF 803

Query: 1163 LLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
            LL E+VERVA MLN+FLL+LVG  P++KS
Sbjct: 804  LLPEMVERVASMLNYFLLQLVG--PQRKS 830



 Score =  193 bits (491), Expect(2) = e-138
 Identities = 104/154 (67%), Positives = 123/154 (79%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   +KD  +YEF PK+LLKQIV IYV++ RGD  +IFPAAI+KDG SYNE LF AA
Sbjct: 826  PQRKSLTVKDPEKYEFRPKQLLKQIVEIYVHIARGDGDNIFPAAISKDGRSYNEQLFTAA 885

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              IL KIG D  +IQ+FV+LG KAK AA+E +DAEA LG+IP+EFLDPI Y+LMKDPVIL
Sbjct: 886  ADILWKIGGDGMIIQDFVQLGVKAKTAASEAMDAEAILGDIPDEFLDPIQYTLMKDPVIL 945

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SR++VDR VI+RHLLS NT PFN SHLT DML
Sbjct: 946  PSSRMSVDRPVIQRHLLSDNTDPFNRSHLTPDML 979


>gb|ONK60080.1| uncharacterized protein A4U43_C08F13970 [Asparagus officinalis]
          Length = 565

 Score =  330 bits (847), Expect(2) = e-138
 Identities = 177/329 (53%), Positives = 221/329 (67%), Gaps = 23/329 (6%)
 Frame = +2

Query: 332  QLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVVWLVGLVGSFELPLS 511
            QL+E I RLE+ +E+ SQ+KLCYEA+I+ D   + RA+SFYRLMVVWLV LVG F++PL 
Sbjct: 45   QLEENIKRLEKIIEILSQQKLCYEAQILRDGALLQRALSFYRLMVVWLVSLVGGFKMPLP 104

Query: 512  TTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIMFMASPKYIRNPYLR 691
            + CP                +  F S   ++LDG +  DF++F IMFMASP Y++NPYLR
Sbjct: 105  SNCPMEFACMPEHFIEDSMDLLIFTSRIPKALDGFVLDDFLSFIIMFMASPSYVKNPYLR 164

Query: 692  AKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDMEFTGNSHTQFYDKF 871
            AKMVEVL+ WMP  SG SS+A +LFEG QLSL +LVRNLLK+YVD+EFTG SHTQFYDKF
Sbjct: 165  AKMVEVLNCWMPERSGLSSIA-SLFEGHQLSLDYLVRNLLKLYVDIEFTG-SHTQFYDKF 222

Query: 872  DIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGSIYLXXXXXXXXXXX 1051
            +IRH IA LL+YLW +P HR AW +IA +EEKG+YLNF NFL+N SI+L           
Sbjct: 223  NIRHNIAGLLEYLWNVPSHRNAWRQIAIKEEKGVYLNFLNFLINDSIFLLDESLNIILEL 282

Query: 1052 XXSDTK-----------------------DLEETIRMHMKSANEDVNMLAYTSDQIAAPF 1162
               + +                         E  +R  MK ANEDV MLA+TS+QI APF
Sbjct: 283  KGIEAEMSNSAEWERRPAQEREERLRLFHSRENIVRFDMKLANEDVGMLAFTSEQIPAPF 342

Query: 1163 LLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
            LL E+VERVA MLN+FLL+LVG  P++KS
Sbjct: 343  LLPEMVERVASMLNYFLLQLVG--PQRKS 369



 Score =  193 bits (491), Expect(2) = e-138
 Identities = 104/154 (67%), Positives = 123/154 (79%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   +KD  +YEF PK+LLKQIV IYV++ RGD  +IFPAAI+KDG SYNE LF AA
Sbjct: 365  PQRKSLTVKDPEKYEFRPKQLLKQIVEIYVHIARGDGDNIFPAAISKDGRSYNEQLFTAA 424

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              IL KIG D  +IQ+FV+LG KAK AA+E +DAEA LG+IP+EFLDPI Y+LMKDPVIL
Sbjct: 425  ADILWKIGGDGMIIQDFVQLGVKAKTAASEAMDAEAILGDIPDEFLDPIQYTLMKDPVIL 484

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SR++VDR VI+RHLLS NT PFN SHLT DML
Sbjct: 485  PSSRMSVDRPVIQRHLLSDNTDPFNRSHLTPDML 518


>gb|ESR44493.1| hypothetical protein CICLE_v10010958mg [Citrus clementina]
          Length = 732

 Score =  353 bits (905), Expect(3) = e-131
 Identities = 190/335 (56%), Positives = 232/335 (69%), Gaps = 23/335 (6%)
 Frame = +2

Query: 314  KSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVVWLVGLVGS 493
            ++PS +QL+  I R+E+E+EL SQEKLCYEA+I+ D   I  A+SFYRLM+VWLV LVG 
Sbjct: 255  QTPS-SQLNLEITRIEKEIELSSQEKLCYEAQILRDGDLIQHALSFYRLMIVWLVDLVGG 313

Query: 494  FELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIMFMASPKYI 673
            F++PL  TCP                +  FAS   ++LDG+L  DFMNF IMFMASPKYI
Sbjct: 314  FKMPLPDTCPMEFACMPEHFVEDAMELLIFASRIPKALDGVLLDDFMNFIIMFMASPKYI 373

Query: 674  RNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDMEFTGNSHT 853
            RNPYLR+KMVEVL+ WMP  SGSSS   TLFEG Q+SL++LVRNLLK+YVD+EFTG SHT
Sbjct: 374  RNPYLRSKMVEVLNCWMPRRSGSSSATATLFEGHQMSLEYLVRNLLKLYVDIEFTG-SHT 432

Query: 854  QFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGSIYLXXXXX 1033
            QFYDKF+IRH IAELL+YLWQ+P HR AW +IA EEEKG+YLNF NFL+N SIYL     
Sbjct: 433  QFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEEEKGVYLNFLNFLINDSIYLLDESL 492

Query: 1034 XXXXXXXXSDTK-----------------------DLEETIRMHMKSANEDVNMLAYTSD 1144
                     + +                         E  IR+ MK ANEDV+MLA+TS+
Sbjct: 493  NKILELKVIEAEMSNTAEWERRPAQERQERTRLFHSQENIIRIDMKLANEDVSMLAFTSE 552

Query: 1145 QIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
            QI APFLL E++ERVA MLN+FLL+LVG  P++KS
Sbjct: 553  QIVAPFLLPEMIERVASMLNYFLLQLVG--PQRKS 585



 Score =  141 bits (355), Expect(3) = e-131
 Identities = 73/109 (66%), Positives = 88/109 (80%), Gaps = 1/109 (0%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK+LLKQIV IYV+L RGD  ++FPAAI+ DG SYNE LF+AA
Sbjct: 581  PQRKSLTLKDPEKYEFRPKQLLKQIVCIYVHLARGDTQNLFPAAISSDGRSYNEQLFSAA 640

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPI 1557
              +L KIGED R+IQEF+ELG KAK AA+E +DAEAALG+IP+EFLDPI
Sbjct: 641  ADVLWKIGEDGRIIQEFIELGAKAKAAASEAMDAEAALGDIPDEFLDPI 689



 Score = 28.5 bits (62), Expect(3) = e-131
 Identities = 13/17 (76%), Positives = 15/17 (88%), Gaps = 1/17 (5%)
 Frame = +3

Query: 1560 VQFDEGSGYFTF-KDHS 1607
            V FDEGSG+FTF K+HS
Sbjct: 716  VHFDEGSGHFTFVKNHS 732


>ref|XP_012084776.1| probable ubiquitin conjugation factor E4 isoform X2 [Jatropha curcas]
 gb|KDP27202.1| hypothetical protein JCGZ_19901 [Jatropha curcas]
          Length = 1026

 Score =  363 bits (933), Expect = e-110
 Identities = 204/379 (53%), Positives = 253/379 (66%), Gaps = 25/379 (6%)
 Frame = +2

Query: 290  LKSMQMLFKSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVV 469
            L +++ + +     QL+  IARLE++LELYSQEKLCYEA+I+ D+ FI RA+SFYRL+VV
Sbjct: 494  LSTLRAMQEQAPAQQLESDIARLEKDLELYSQEKLCYEAQILRDEAFIQRALSFYRLLVV 553

Query: 470  WLVGLVGSFELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIM 649
            WLVGLVG F++PL  TCP                +  FAS    +LDG+L  DFMNF IM
Sbjct: 554  WLVGLVGGFKMPLPPTCPMEFASLPEHFVEDAMELLIFASRIPRALDGVLLDDFMNFIIM 613

Query: 650  FMASPKYIRNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDM 829
            FMASP YIRNPYLRAKMVEVL+ WMP  SGSS+ A TLFEG QLSL++LVRNLLK+YVD+
Sbjct: 614  FMASPTYIRNPYLRAKMVEVLNCWMPRRSGSSATA-TLFEGHQLSLEYLVRNLLKLYVDI 672

Query: 830  EFTGNSHTQFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGS 1009
            EFTG SHTQFYDKF+IRH IAELL+YLWQ+P HR AW +IA EEEKG+YLNF NFL+N S
Sbjct: 673  EFTG-SHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEEEKGVYLNFLNFLINDS 731

Query: 1010 IYLXXXXXXXXXXXXXSDTK-----------------------DLEETIRMHMKSANEDV 1120
            IYL              + +                         E  IR+ MK ANEDV
Sbjct: 732  IYLLDESLNKILELKELEAEMSNTAEWERRPAQERQERTRLFHSQENIIRIDMKLANEDV 791

Query: 1121 NMLAYTSDQIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKSQN*K-MQDMNFSPNAC* 1297
            +MLA+TS+QI APFLL E+V+RVA MLN+FLL+LVG  P++KS + K  +   F P    
Sbjct: 792  SMLAFTSEQITAPFLLPEMVDRVASMLNYFLLQLVG--PQRKSLSLKDPEKYEFRPKQLL 849

Query: 1298 SRL-STYMSI*KGAIKPIF 1351
             ++   Y+ + KG  + IF
Sbjct: 850  KQIVDIYVHLAKGDTENIF 868



 Score =  206 bits (524), Expect = 9e-53
 Identities = 108/154 (70%), Positives = 127/154 (82%), Gaps = 2/154 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNEALFNAA 1410
            P+ K   LKD  +YEF PK+LLKQIV+IYV+L +GD  +IFP+AI+KDG SYNE LFNAA
Sbjct: 828  PQRKSLSLKDPEKYEFRPKQLLKQIVDIYVHLAKGDTENIFPSAISKDGRSYNEQLFNAA 887

Query: 1411 PIILRKIGEDERVIQEFVELGRKAKVAANETLDAEAALGEIPEEFLDPIWYSLMKDPVIL 1590
              +LR+IGED RV  EF+ELG++AKVAA+E +D EAALGEIP+EFLDPI Y+LMKDPVIL
Sbjct: 888  ADVLRRIGEDGRVTHEFIELGKRAKVAASEAMDTEAALGEIPDEFLDPIQYTLMKDPVIL 947

Query: 1591 P-SRITVDRSVIERHLLSFNTVPFNHSHLTVDML 1689
            P SRITVDR VI+RHLLS  T PFN SHLT DML
Sbjct: 948  PSSRITVDRPVIQRHLLSDATDPFNRSHLTADML 981


>gb|PHT29598.1| putative ubiquitin conjugation factor E4 [Capsicum baccatum]
          Length = 937

 Score =  358 bits (919), Expect(2) = e-109
 Identities = 192/343 (55%), Positives = 237/343 (69%), Gaps = 23/343 (6%)
 Frame = +2

Query: 290  LKSMQMLFKSPSDTQLDEGIARLEQELELYSQEKLCYEAEIIEDKGFIHRAVSFYRLMVV 469
            L +M+ + +     QL + I+RLE+ELELYSQEKLCYEA+I+ D G + RA+S+YRLMVV
Sbjct: 507  LSTMKTMLEQAPSPQLQQEISRLEKELELYSQEKLCYEAQILRDGGLLQRALSYYRLMVV 566

Query: 470  WLVGLVGSFELPLSTTCPQGICFFA*ALCGRCNGVAYFASWNSESLDGLLHKDFMNFTIM 649
            WLVGLVG F++PL + CP                +  FAS    +LDG+L  DFMNF IM
Sbjct: 567  WLVGLVGGFKMPLPSPCPMEFASMPEHFVEDAMELLIFASRIPRALDGVLLDDFMNFIIM 626

Query: 650  FMASPKYIRNPYLRAKMVEVLHSWMPSDSGSSSVAVTLFEGQQLSLKFLVRNLLKVYVDM 829
            FMASP+YIRNPYLRAKMVEVL+ WMP  SGS++ + TLFEG QLSL++LVRNLLK+YVD+
Sbjct: 627  FMASPEYIRNPYLRAKMVEVLNCWMPRRSGSTATS-TLFEGHQLSLEYLVRNLLKLYVDI 685

Query: 830  EFTGNSHTQFYDKFDIRHKIAELLQYLWQIPGHRKAWLKIA*EEEKGIYLNFFNFLVNGS 1009
            EFTG SHTQFYDKF+IRH IAELL+YLWQ+P HR AW +IA EEEKG+YLNF NFL+N S
Sbjct: 686  EFTG-SHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEEEKGVYLNFLNFLINDS 744

Query: 1010 IYLXXXXXXXXXXXXXSDTK-----------------------DLEETIRMHMKSANEDV 1120
            IYL              + +                         E  IR+ MK ANEDV
Sbjct: 745  IYLLDESLNKILELKELEAEMSNTAEWERRPAQERQERTRLFHSQENIIRIDMKLANEDV 804

Query: 1121 NMLAYTSDQIAAPFLLSEVVERVAGMLNFFLLKLVGRPPEKKS 1249
            ++LA+TS+QI  PFLL E+VERVA MLN+FLL+LVG  P++KS
Sbjct: 805  SLLAFTSEQITVPFLLPEMVERVASMLNYFLLQLVG--PQRKS 845



 Score = 68.9 bits (167), Expect(2) = e-109
 Identities = 36/54 (66%), Positives = 40/54 (74%), Gaps = 1/54 (1%)
 Frame = +1

Query: 1234 PREKISKLKDA-RYEFFPKRLLKQIVNIYVNLERGDKADIFPAAIAKDGGSYNE 1392
            P+ K   LKD  +YEF PK LLKQIV IYV+L RGDK  IFPAAI +DG SYNE
Sbjct: 841  PQRKSLSLKDPEKYEFRPKELLKQIVKIYVHLARGDKEKIFPAAIIRDGRSYNE 894