BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Acanthopanax24_contig00012517
         (952 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|KZN11953.1| hypothetical protein DCAR_004609 [Daucus carota s...   150   1e-36
ref|XP_017230627.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   150   1e-36
gb|KZN07967.1| hypothetical protein DCAR_000636 [Daucus carota s...   147   1e-35
ref|XP_017237143.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   147   1e-35
ref|XP_017237104.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   147   1e-35
gb|ONI07224.1| hypothetical protein PRUPE_5G107000 [Prunus persica]   109   8e-23
ref|XP_020419685.1| E4 SUMO-protein ligase PIAL2 [Prunus persica...   109   8e-23
ref|XP_008239090.1| PREDICTED: E4 SUMO-protein ligase PIAL2 [Pru...   108   1e-22
ref|XP_017192140.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   107   4e-22
ref|XP_024170804.1| E4 SUMO-protein ligase PIAL1 isoform X4 [Ros...   107   5e-22
ref|XP_024170803.1| E4 SUMO-protein ligase PIAL2 isoform X3 [Ros...   107   5e-22
ref|XP_008375961.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   107   5e-22
ref|XP_008348548.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   107   5e-22
ref|XP_024170802.1| E4 SUMO-protein ligase PIAL2 isoform X2 [Ros...   107   5e-22
ref|XP_024170801.1| E4 SUMO-protein ligase PIAL2 isoform X1 [Ros...   107   5e-22
ref|XP_004300819.1| PREDICTED: uncharacterized protein LOC101293...   107   6e-22
ref|XP_023911115.1| E4 SUMO-protein ligase PIAL2-like isoform X2...   105   2e-21
ref|XP_023911114.1| E4 SUMO-protein ligase PIAL2-like isoform X1...   105   2e-21
ref|XP_022737523.1| E4 SUMO-protein ligase PIAL2-like isoform X3...   103   7e-21
ref|XP_022737522.1| E4 SUMO-protein ligase PIAL2-like isoform X2...   103   7e-21

>gb|KZN11953.1| hypothetical protein DCAR_004609 [Daucus carota subsp. sativus]
          Length = 1030

 Score =  150 bits (378), Expect = 1e-36
 Identities = 90/186 (48%), Positives = 109/186 (58%)
 Frame = +1

Query: 28   PSGYRVSPVLATESHQHQAVSQRMPHLRTQSPSLNRSSAHLPQSQTHQGGLQNRVSPTPV 207
            P GYRV       SHQ     QRMPH+R+QSP L R+  HLP +QTH GG+ NR S   V
Sbjct: 652  PGGYRVPFSNGQNSHQQHFTGQRMPHIRSQSPGLARTPPHLPPTQTHHGGMPNRASSPYV 711

Query: 208  GGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPSGGNTRVPVQPV 387
               Q + F                   VQ+QT    + +  N+D +RPS  NT   +QPV
Sbjct: 712  --RQQEQFHSFSQRTSHLPRTPSLFNPVQLQT----TPMPRNVDAHRPSISNTGGNLQPV 765

Query: 388  TRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQPAARPLASLSTSL 567
              P+G V++S  EQDWRPTGRMRGSLSG+AYS AL+QYI  PTQPVQP  RP  +  TS 
Sbjct: 766  GTPEGTVNTST-EQDWRPTGRMRGSLSGRAYSEALNQYIIQPTQPVQP-IRPPTNTVTSP 823

Query: 568  SAVPSP 585
            S +PSP
Sbjct: 824  SGIPSP 829


>ref|XP_017230627.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like [Daucus carota subsp.
            sativus]
          Length = 1031

 Score =  150 bits (378), Expect = 1e-36
 Identities = 90/186 (48%), Positives = 109/186 (58%)
 Frame = +1

Query: 28   PSGYRVSPVLATESHQHQAVSQRMPHLRTQSPSLNRSSAHLPQSQTHQGGLQNRVSPTPV 207
            P GYRV       SHQ     QRMPH+R+QSP L R+  HLP +QTH GG+ NR S   V
Sbjct: 653  PGGYRVPFSNGQNSHQQHFTGQRMPHIRSQSPGLARTPPHLPPTQTHHGGMPNRASSPYV 712

Query: 208  GGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPSGGNTRVPVQPV 387
               Q + F                   VQ+QT    + +  N+D +RPS  NT   +QPV
Sbjct: 713  --RQQEQFHSFSQRTSHLPRTPSLFNPVQLQT----TPMPRNVDAHRPSISNTGGNLQPV 766

Query: 388  TRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQPAARPLASLSTSL 567
              P+G V++S  EQDWRPTGRMRGSLSG+AYS AL+QYI  PTQPVQP  RP  +  TS 
Sbjct: 767  GTPEGTVNTST-EQDWRPTGRMRGSLSGRAYSEALNQYIIQPTQPVQP-IRPPTNTVTSP 824

Query: 568  SAVPSP 585
            S +PSP
Sbjct: 825  SGIPSP 830


>gb|KZN07967.1| hypothetical protein DCAR_000636 [Daucus carota subsp. sativus]
          Length = 840

 Score =  147 bits (370), Expect = 1e-35
 Identities = 90/195 (46%), Positives = 114/195 (58%), Gaps = 2/195 (1%)
 Frame = +1

Query: 19   SQHPSGYRVSPVLATESHQ--HQAVSQRMPHLRTQSPSLNRSSAHLPQSQTHQGGLQNRV 192
            S+HP GYRV  V  T+ H    Q++ QRM ++R+ SPSL RSS H P +Q+  GGL NRV
Sbjct: 612  SRHPRGYRVPTVPVTDGHNLHQQSIGQRMLNIRSPSPSLVRSSVHGPPTQSPHGGLLNRV 671

Query: 193  SPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPSGGNTRV 372
            + TP  G Q                      S  VQ P    ++  +    RP  GNT  
Sbjct: 672  TSTPPVGQQPGQSHRSAQQASQLAGISSQMLSGLVQEPPIIRNVSADS---RPLTGNTGG 728

Query: 373  PVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQPAARPLAS 552
             +QP T P+ LVD S A Q+WRPTGRMRGSLSG AYSAAL+Q+I+ P QPVQ   RPL +
Sbjct: 729  TIQPAT-PEVLVDMSAAGQEWRPTGRMRGSLSGAAYSAALNQFITQPAQPVQ-TTRPLTN 786

Query: 553  LSTSLSAVPSPPHGL 597
            ++TS+S +P P H L
Sbjct: 787  VATSISGIPYPQHVL 801


>ref|XP_017237143.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X2 [Daucus carota
            subsp. sativus]
          Length = 883

 Score =  147 bits (370), Expect = 1e-35
 Identities = 90/195 (46%), Positives = 114/195 (58%), Gaps = 2/195 (1%)
 Frame = +1

Query: 19   SQHPSGYRVSPVLATESHQ--HQAVSQRMPHLRTQSPSLNRSSAHLPQSQTHQGGLQNRV 192
            S+HP GYRV  V  T+ H    Q++ QRM ++R+ SPSL RSS H P +Q+  GGL NRV
Sbjct: 655  SRHPRGYRVPTVPVTDGHNLHQQSIGQRMLNIRSPSPSLVRSSVHGPPTQSPHGGLLNRV 714

Query: 193  SPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPSGGNTRV 372
            + TP  G Q                      S  VQ P    ++  +    RP  GNT  
Sbjct: 715  TSTPPVGQQPGQSHRSAQQASQLAGISSQMLSGLVQEPPIIRNVSADS---RPLTGNTGG 771

Query: 373  PVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQPAARPLAS 552
             +QP T P+ LVD S A Q+WRPTGRMRGSLSG AYSAAL+Q+I+ P QPVQ   RPL +
Sbjct: 772  TIQPAT-PEVLVDMSAAGQEWRPTGRMRGSLSGAAYSAALNQFITQPAQPVQ-TTRPLTN 829

Query: 553  LSTSLSAVPSPPHGL 597
            ++TS+S +P P H L
Sbjct: 830  VATSISGIPYPQHVL 844


>ref|XP_017237104.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Daucus carota
            subsp. sativus]
          Length = 885

 Score =  147 bits (370), Expect = 1e-35
 Identities = 90/195 (46%), Positives = 114/195 (58%), Gaps = 2/195 (1%)
 Frame = +1

Query: 19   SQHPSGYRVSPVLATESHQ--HQAVSQRMPHLRTQSPSLNRSSAHLPQSQTHQGGLQNRV 192
            S+HP GYRV  V  T+ H    Q++ QRM ++R+ SPSL RSS H P +Q+  GGL NRV
Sbjct: 657  SRHPRGYRVPTVPVTDGHNLHQQSIGQRMLNIRSPSPSLVRSSVHGPPTQSPHGGLLNRV 716

Query: 193  SPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPSGGNTRV 372
            + TP  G Q                      S  VQ P    ++  +    RP  GNT  
Sbjct: 717  TSTPPVGQQPGQSHRSAQQASQLAGISSQMLSGLVQEPPIIRNVSADS---RPLTGNTGG 773

Query: 373  PVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQPAARPLAS 552
             +QP T P+ LVD S A Q+WRPTGRMRGSLSG AYSAAL+Q+I+ P QPVQ   RPL +
Sbjct: 774  TIQPAT-PEVLVDMSAAGQEWRPTGRMRGSLSGAAYSAALNQFITQPAQPVQ-TTRPLTN 831

Query: 553  LSTSLSAVPSPPHGL 597
            ++TS+S +P P H L
Sbjct: 832  VATSISGIPYPQHVL 846


>gb|ONI07224.1| hypothetical protein PRUPE_5G107000 [Prunus persica]
          Length = 854

 Score =  109 bits (273), Expect = 8e-23
 Identities = 83/200 (41%), Positives = 101/200 (50%), Gaps = 3/200 (1%)
 Frame = +1

Query: 10   PALSQHPSGYRVSPVLAT--ESHQHQAVSQ-RMPHLRTQSPSLNRSSAHLPQSQTHQGGL 180
            P  SQ  S  R SP L     +H  QA +  R P    QS S  RSS+HL ++   QG  
Sbjct: 652  PVPSQLQSANRASPGLMDFQNAHLQQAFNNARTPQTMGQSSSSIRSSSHLSRAHIQQGNA 711

Query: 181  QNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPSGG 360
            Q     T    +    F               PS  VQ QTPRT  S+ VN+ G      
Sbjct: 712  QVGTGQTSSSLNNQQRFKAGTHLAAIMARQS-PSMPVQNQTPRTRPSLPVNVGG------ 764

Query: 361  NTRVPVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQPAAR 540
                 +Q V+  DG VD S +EQ+WRPTGRMRGSLSG+AYSAA  Q+I  PTQP Q AAR
Sbjct: 765  ----TMQAVSGADGSVDLS-SEQNWRPTGRMRGSLSGRAYSAAFHQFIIAPTQPTQ-AAR 818

Query: 541  PLASLSTSLSAVPSPPHGLM 600
            P  +L++    VPS P  L+
Sbjct: 819  PPPNLTSPPPVVPSQPETLI 838


>ref|XP_020419685.1| E4 SUMO-protein ligase PIAL2 [Prunus persica]
 gb|ONI07225.1| hypothetical protein PRUPE_5G107000 [Prunus persica]
          Length = 893

 Score =  109 bits (273), Expect = 8e-23
 Identities = 83/200 (41%), Positives = 101/200 (50%), Gaps = 3/200 (1%)
 Frame = +1

Query: 10   PALSQHPSGYRVSPVLAT--ESHQHQAVSQ-RMPHLRTQSPSLNRSSAHLPQSQTHQGGL 180
            P  SQ  S  R SP L     +H  QA +  R P    QS S  RSS+HL ++   QG  
Sbjct: 691  PVPSQLQSANRASPGLMDFQNAHLQQAFNNARTPQTMGQSSSSIRSSSHLSRAHIQQGNA 750

Query: 181  QNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPSGG 360
            Q     T    +    F               PS  VQ QTPRT  S+ VN+ G      
Sbjct: 751  QVGTGQTSSSLNNQQRFKAGTHLAAIMARQS-PSMPVQNQTPRTRPSLPVNVGG------ 803

Query: 361  NTRVPVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQPAAR 540
                 +Q V+  DG VD S +EQ+WRPTGRMRGSLSG+AYSAA  Q+I  PTQP Q AAR
Sbjct: 804  ----TMQAVSGADGSVDLS-SEQNWRPTGRMRGSLSGRAYSAAFHQFIIAPTQPTQ-AAR 857

Query: 541  PLASLSTSLSAVPSPPHGLM 600
            P  +L++    VPS P  L+
Sbjct: 858  PPPNLTSPPPVVPSQPETLI 877


>ref|XP_008239090.1| PREDICTED: E4 SUMO-protein ligase PIAL2 [Prunus mume]
          Length = 844

 Score =  108 bits (271), Expect = 1e-22
 Identities = 82/200 (41%), Positives = 102/200 (51%), Gaps = 3/200 (1%)
 Frame = +1

Query: 10   PALSQHPSGYRVSPVLAT--ESHQHQAVSQ-RMPHLRTQSPSLNRSSAHLPQSQTHQGGL 180
            P  SQ  S  R SP L     +H  QA +  R P    QS S  RSS+HL ++   QG  
Sbjct: 642  PVPSQLQSANRASPGLMDFQNAHLQQAFNNARTPQTMGQSSSSIRSSSHLSRAHIQQGNA 701

Query: 181  QNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPSGG 360
            Q     T    +    F+              PS  VQ QT RT  S+ VN+ G      
Sbjct: 702  QVGTGQTSSSLNNQQRFMAGAHLAAMMARQS-PSMPVQNQTQRTRPSLPVNVGG------ 754

Query: 361  NTRVPVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQPAAR 540
                 +Q V+  DG VD S +EQ+WRPTGRMRGSLSG+AYSAA +Q+I  PTQP Q AAR
Sbjct: 755  ----TMQAVSGADGSVDLS-SEQNWRPTGRMRGSLSGRAYSAAFNQFIIAPTQPTQ-AAR 808

Query: 541  PLASLSTSLSAVPSPPHGLM 600
            P  +L++    VPS P  L+
Sbjct: 809  PPPNLTSPPPVVPSQPQTLI 828


>ref|XP_017192140.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like, partial [Malus
            domestica]
          Length = 677

 Score =  107 bits (267), Expect = 4e-22
 Identities = 76/200 (38%), Positives = 99/200 (49%), Gaps = 6/200 (3%)
 Frame = +1

Query: 10   PALSQHPSGYRVSPVLAT--ESHQHQAVSQRMPHLRTQSPSLNRSSAHLPQSQTHQGGLQ 183
            PA SQ  S  R S  L     +H  QA + R P    Q  S NRSS+H+ ++   QG ++
Sbjct: 461  PAPSQLQSVNRTSLGLMEFQNAHLQQAFNPRTPQTVGQLSSANRSSSHVSRAHIQQGSVR 520

Query: 184  NRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPSG-- 357
                 T         F +             PS  VQ Q+PR    +  N +G+R +   
Sbjct: 521  VETGQTSSSLDNQQRFAIGQQQRVAMMARQSPSTPVQNQSPRNRPILPANAEGFRTAALE 580

Query: 358  --GNTRVPVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQP 531
               N    VQ V+  DG  D S  E +WRPTGRMRGSLSG+AYSAAL+Q+I  PTQP   
Sbjct: 581  QSRNVVETVQAVSGADGSPDLSSGE-NWRPTGRMRGSLSGRAYSAALNQFIIQPTQPTPA 639

Query: 532  AARPLASLSTSLSAVPSPPH 591
            A+RP ++LS       +PPH
Sbjct: 640  ASRPPSNLS-------APPH 652


>ref|XP_024170804.1| E4 SUMO-protein ligase PIAL1 isoform X4 [Rosa chinensis]
          Length = 779

 Score =  107 bits (267), Expect = 5e-22
 Identities = 86/207 (41%), Positives = 110/207 (53%), Gaps = 13/207 (6%)
 Frame = +1

Query: 10   PALSQHPSGYRVSPVLA--TESHQHQAVSQRMPHLRTQSPSLNRSS-AHLPQSQTHQGGL 180
            P  SQ  S  RVS  L   T +H  Q  + R PH     P++ +SS AH  +S   QG  
Sbjct: 538  PVPSQLQSASRVSSGLQGFTNAHLQQTFNPR-PH-----PTVGQSSSAHSSRSHVMQGSA 591

Query: 181  QNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSP-SVQVQTPRTSSSIHVNIDGYRPSG 357
            Q  +  TP   +    F++              SP + Q QTPRT  S+ VN  G+R S 
Sbjct: 592  QVGIGQTPSSLNNQQSFIMAAQRAAQARQS---SPMTAQNQTPRTRPSLSVNAAGFRGSA 648

Query: 358  GNTRV----PVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPV 525
            G+ R      VQ V+  DGL  +S +EQ+WRPTGRMRGSLSGQAYSAAL+Q+I  PTQP 
Sbjct: 649  GDQRGNIGGTVQAVSGADGL-GNSPSEQNWRPTGRMRGSLSGQAYSAALNQFIIRPTQPT 707

Query: 526  QPAARPLASLS-----TSLSAVPSPPH 591
            +  ARP   L+     T+ S +  PPH
Sbjct: 708  E-TARPAPHLTPPSHLTTPSHLTPPPH 733


>ref|XP_024170803.1| E4 SUMO-protein ligase PIAL2 isoform X3 [Rosa chinensis]
          Length = 895

 Score =  107 bits (267), Expect = 5e-22
 Identities = 86/207 (41%), Positives = 110/207 (53%), Gaps = 13/207 (6%)
 Frame = +1

Query: 10   PALSQHPSGYRVSPVLA--TESHQHQAVSQRMPHLRTQSPSLNRSS-AHLPQSQTHQGGL 180
            P  SQ  S  RVS  L   T +H  Q  + R PH     P++ +SS AH  +S   QG  
Sbjct: 654  PVPSQLQSASRVSSGLQGFTNAHLQQTFNPR-PH-----PTVGQSSSAHSSRSHVMQGSA 707

Query: 181  QNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSP-SVQVQTPRTSSSIHVNIDGYRPSG 357
            Q  +  TP   +    F++              SP + Q QTPRT  S+ VN  G+R S 
Sbjct: 708  QVGIGQTPSSLNNQQSFIMAAQRAAQARQS---SPMTAQNQTPRTRPSLSVNAAGFRGSA 764

Query: 358  GNTRV----PVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPV 525
            G+ R      VQ V+  DGL  +S +EQ+WRPTGRMRGSLSGQAYSAAL+Q+I  PTQP 
Sbjct: 765  GDQRGNIGGTVQAVSGADGL-GNSPSEQNWRPTGRMRGSLSGQAYSAALNQFIIRPTQPT 823

Query: 526  QPAARPLASLS-----TSLSAVPSPPH 591
            +  ARP   L+     T+ S +  PPH
Sbjct: 824  E-TARPAPHLTPPSHLTTPSHLTPPPH 849


>ref|XP_008375961.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like [Malus domestica]
          Length = 896

 Score =  107 bits (267), Expect = 5e-22
 Identities = 76/200 (38%), Positives = 100/200 (50%), Gaps = 6/200 (3%)
 Frame = +1

Query: 10   PALSQHPSGYRVSPVLAT--ESHQHQAVSQRMPHLRTQSPSLNRSSAHLPQSQTHQGGLQ 183
            PA SQ  S  R S  L     +H  QA++ R P       S N SS+HL ++Q  QG ++
Sbjct: 680  PAPSQMQSANRTSVGLMEFQNAHLQQALNPRTPQTVGPFSSANGSSSHLSRAQIQQGSVR 739

Query: 184  NRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPS--- 354
                 T    +    F++             PS   Q Q+PR    +  N +G+R +   
Sbjct: 740  VGTGQTSSSLNNQQRFMIAQQQLAAMMSRQSPSTPDQNQSPRNRPILAANAEGFRSAAME 799

Query: 355  -GGNTRVPVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQP 531
             G N    VQ V+  DG  D S  E +WRPTGRMRGSLSG+AYSAAL+Q+I  PTQP   
Sbjct: 800  QGRNIVGTVQAVSGADGSPDLSSGE-NWRPTGRMRGSLSGRAYSAALNQFIIQPTQPTPA 858

Query: 532  AARPLASLSTSLSAVPSPPH 591
            A RP ++LS       +PPH
Sbjct: 859  APRPPSNLS-------APPH 871


>ref|XP_008348548.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like [Malus domestica]
          Length = 896

 Score =  107 bits (267), Expect = 5e-22
 Identities = 76/200 (38%), Positives = 99/200 (49%), Gaps = 6/200 (3%)
 Frame = +1

Query: 10   PALSQHPSGYRVSPVLAT--ESHQHQAVSQRMPHLRTQSPSLNRSSAHLPQSQTHQGGLQ 183
            PA SQ  S  R S  L     +H  QA + R P    Q  S NRSS+H+ ++   QG ++
Sbjct: 680  PAPSQLQSVNRTSLGLMEFQNAHLQQAFNPRTPQTVGQLSSANRSSSHVSRAHIQQGSVR 739

Query: 184  NRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPSG-- 357
                 T         F +             PS  VQ Q+PR    +  N +G+R +   
Sbjct: 740  VETGQTSSSLDNQQRFAIGQQQRVAMMARQSPSTPVQNQSPRNRPILPANAEGFRTAALE 799

Query: 358  --GNTRVPVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQP 531
               N    VQ V+  DG  D S  E +WRPTGRMRGSLSG+AYSAAL+Q+I  PTQP   
Sbjct: 800  QSRNVVETVQAVSGADGSPDLSSGE-NWRPTGRMRGSLSGRAYSAALNQFIIQPTQPTPA 858

Query: 532  AARPLASLSTSLSAVPSPPH 591
            A+RP ++LS       +PPH
Sbjct: 859  ASRPPSNLS-------APPH 871


>ref|XP_024170802.1| E4 SUMO-protein ligase PIAL2 isoform X2 [Rosa chinensis]
 gb|PRQ17579.1| putative E3 SUMO protein ligase [Rosa chinensis]
          Length = 919

 Score =  107 bits (267), Expect = 5e-22
 Identities = 86/207 (41%), Positives = 110/207 (53%), Gaps = 13/207 (6%)
 Frame = +1

Query: 10   PALSQHPSGYRVSPVLA--TESHQHQAVSQRMPHLRTQSPSLNRSS-AHLPQSQTHQGGL 180
            P  SQ  S  RVS  L   T +H  Q  + R PH     P++ +SS AH  +S   QG  
Sbjct: 678  PVPSQLQSASRVSSGLQGFTNAHLQQTFNPR-PH-----PTVGQSSSAHSSRSHVMQGSA 731

Query: 181  QNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSP-SVQVQTPRTSSSIHVNIDGYRPSG 357
            Q  +  TP   +    F++              SP + Q QTPRT  S+ VN  G+R S 
Sbjct: 732  QVGIGQTPSSLNNQQSFIMAAQRAAQARQS---SPMTAQNQTPRTRPSLSVNAAGFRGSA 788

Query: 358  GNTRV----PVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPV 525
            G+ R      VQ V+  DGL  +S +EQ+WRPTGRMRGSLSGQAYSAAL+Q+I  PTQP 
Sbjct: 789  GDQRGNIGGTVQAVSGADGL-GNSPSEQNWRPTGRMRGSLSGQAYSAALNQFIIRPTQPT 847

Query: 526  QPAARPLASLS-----TSLSAVPSPPH 591
            +  ARP   L+     T+ S +  PPH
Sbjct: 848  E-TARPAPHLTPPSHLTTPSHLTPPPH 873


>ref|XP_024170801.1| E4 SUMO-protein ligase PIAL2 isoform X1 [Rosa chinensis]
          Length = 940

 Score =  107 bits (267), Expect = 5e-22
 Identities = 86/207 (41%), Positives = 110/207 (53%), Gaps = 13/207 (6%)
 Frame = +1

Query: 10   PALSQHPSGYRVSPVLA--TESHQHQAVSQRMPHLRTQSPSLNRSS-AHLPQSQTHQGGL 180
            P  SQ  S  RVS  L   T +H  Q  + R PH     P++ +SS AH  +S   QG  
Sbjct: 699  PVPSQLQSASRVSSGLQGFTNAHLQQTFNPR-PH-----PTVGQSSSAHSSRSHVMQGSA 752

Query: 181  QNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSP-SVQVQTPRTSSSIHVNIDGYRPSG 357
            Q  +  TP   +    F++              SP + Q QTPRT  S+ VN  G+R S 
Sbjct: 753  QVGIGQTPSSLNNQQSFIMAAQRAAQARQS---SPMTAQNQTPRTRPSLSVNAAGFRGSA 809

Query: 358  GNTRV----PVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPV 525
            G+ R      VQ V+  DGL  +S +EQ+WRPTGRMRGSLSGQAYSAAL+Q+I  PTQP 
Sbjct: 810  GDQRGNIGGTVQAVSGADGL-GNSPSEQNWRPTGRMRGSLSGQAYSAALNQFIIRPTQPT 868

Query: 526  QPAARPLASLS-----TSLSAVPSPPH 591
            +  ARP   L+     T+ S +  PPH
Sbjct: 869  E-TARPAPHLTPPSHLTTPSHLTPPPH 894


>ref|XP_004300819.1| PREDICTED: uncharacterized protein LOC101293006 [Fragaria vesca
            subsp. vesca]
          Length = 884

 Score =  107 bits (266), Expect = 6e-22
 Identities = 82/197 (41%), Positives = 104/197 (52%), Gaps = 6/197 (3%)
 Frame = +1

Query: 19   SQHPSGYRVSPVLA--TESHQHQAVSQRMPHLRTQSPSLNRSSAHLPQSQTHQGGLQNRV 192
            SQ  S  RVS  L   T +H  +  + R P   +QSP +     H  +S   QG  Q  +
Sbjct: 669  SQLQSASRVSSGLQGFTNAHLDKTFNSRTPPAMSQSPRV-----HTLRSHVRQGSAQVGI 723

Query: 193  SPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPSGGNTRV 372
            +  P   +    F V             P P+ Q QTPRT  S+ VN  G+R S G+ R 
Sbjct: 724  NQAPSSLNNQQSFTVAAQRAAMARQSS-PMPA-QNQTPRTRPSLSVNAGGFRGSAGDRRG 781

Query: 373  ----PVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQPAAR 540
                PVQ V+  D LVDS  +EQ+WRPTGRMRGSLSGQAY+AAL+++I  PT P +  AR
Sbjct: 782  NIGGPVQAVSGADELVDSP-SEQNWRPTGRMRGSLSGQAYTAALNKFIIRPTPPTE-KAR 839

Query: 541  PLASLSTSLSAVPSPPH 591
            P AS  T  S +  PPH
Sbjct: 840  P-ASHLTPPSHLTRPPH 855


>ref|XP_023911115.1| E4 SUMO-protein ligase PIAL2-like isoform X2 [Quercus suber]
 gb|POF11949.1| e4 sumo-protein ligase pial2 [Quercus suber]
          Length = 921

 Score =  105 bits (263), Expect = 2e-21
 Identities = 79/197 (40%), Positives = 103/197 (52%), Gaps = 8/197 (4%)
 Frame = +1

Query: 13   ALSQHPSGYRVSPVLATE---SHQHQAVSQRMPHLRTQSPSLNRSSAHLPQSQTHQGGLQ 183
            A S+  S Y+ +  L TE   SH  QA + RMP    QS S    S+H  ++Q  QGG Q
Sbjct: 705  AQSRLQSHYKATSGLLTEFQNSHLQQAFNPRMPQSVGQSSSPIPPSSHPSRTQVQQGGGQ 764

Query: 184  NRVSPTP-VGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPSGG 360
              +S      G Q   +LV             PS  +  QT RT  S  V+ DG R S G
Sbjct: 765  VGISQRAGTTGSQPARYLVNQNRYHPQNSRQAPSVPLLNQTSRTGQSRPVSADGLRASTG 824

Query: 361  NTRVPV----QPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQ 528
              R  +    Q V R DGL+DS+ +EQ+WRP+GRMRGSL+G+AYS ALSQ++  PTQ  Q
Sbjct: 825  EQRGNMGGMSQAVPRADGLLDST-SEQNWRPSGRMRGSLAGRAYSDALSQFMIQPTQTAQ 883

Query: 529  PAARPLASLSTSLSAVP 579
             A  P  S   + + +P
Sbjct: 884  TARPPPNSTPPASNVLP 900


>ref|XP_023911114.1| E4 SUMO-protein ligase PIAL2-like isoform X1 [Quercus suber]
          Length = 948

 Score =  105 bits (263), Expect = 2e-21
 Identities = 79/197 (40%), Positives = 103/197 (52%), Gaps = 8/197 (4%)
 Frame = +1

Query: 13   ALSQHPSGYRVSPVLATE---SHQHQAVSQRMPHLRTQSPSLNRSSAHLPQSQTHQGGLQ 183
            A S+  S Y+ +  L TE   SH  QA + RMP    QS S    S+H  ++Q  QGG Q
Sbjct: 732  AQSRLQSHYKATSGLLTEFQNSHLQQAFNPRMPQSVGQSSSPIPPSSHPSRTQVQQGGGQ 791

Query: 184  NRVSPTP-VGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPSGG 360
              +S      G Q   +LV             PS  +  QT RT  S  V+ DG R S G
Sbjct: 792  VGISQRAGTTGSQPARYLVNQNRYHPQNSRQAPSVPLLNQTSRTGQSRPVSADGLRASTG 851

Query: 361  NTRVPV----QPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQ 528
              R  +    Q V R DGL+DS+ +EQ+WRP+GRMRGSL+G+AYS ALSQ++  PTQ  Q
Sbjct: 852  EQRGNMGGMSQAVPRADGLLDST-SEQNWRPSGRMRGSLAGRAYSDALSQFMIQPTQTAQ 910

Query: 529  PAARPLASLSTSLSAVP 579
             A  P  S   + + +P
Sbjct: 911  TARPPPNSTPPASNVLP 927


>ref|XP_022737523.1| E4 SUMO-protein ligase PIAL2-like isoform X3 [Durio zibethinus]
          Length = 893

 Score =  103 bits (258), Expect = 7e-21
 Identities = 78/199 (39%), Positives = 105/199 (52%), Gaps = 6/199 (3%)
 Frame = +1

Query: 13   ALSQHPSGYRVSPVLATE---SHQHQAVSQRMPHLRTQSPSLNRSSAHLPQSQTHQGGLQ 183
            A SQ P  YR S  L  E    H  QA++ R+   R+ SP L RS + L ++QT QG  Q
Sbjct: 658  ASSQLPGSYRASSGLLGEYQNPHLQQALNPRVSQPRSPSPGLIRSPSPLLRTQTQQGAAQ 717

Query: 184  NRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPSGGN 363
              +  T    +      +             P  +VQ Q+ R ++S   N+DG R S   
Sbjct: 718  VGLGYTAGNVNSNPTRFMAAAQRATQMARQPPMVAVQTQSSR-AASYSGNVDGSRASAVE 776

Query: 364  TRVPVQPVTRPDGLVDSS---VAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQPA 534
             R+ +  +T      D+S   V+EQ+WRPTGRMRGSL+G+AYS ALSQ +  PTQ  Q A
Sbjct: 777  QRLNMGGLTPAASRADTSADLVSEQNWRPTGRMRGSLTGRAYSEALSQLMIQPTQSAQ-A 835

Query: 535  ARPLASLSTSLSAVPSPPH 591
            ARP A+L++S S    PPH
Sbjct: 836  ARPQANLTSSPSV---PPH 851


>ref|XP_022737522.1| E4 SUMO-protein ligase PIAL2-like isoform X2 [Durio zibethinus]
          Length = 914

 Score =  103 bits (258), Expect = 7e-21
 Identities = 78/199 (39%), Positives = 105/199 (52%), Gaps = 6/199 (3%)
 Frame = +1

Query: 13   ALSQHPSGYRVSPVLATE---SHQHQAVSQRMPHLRTQSPSLNRSSAHLPQSQTHQGGLQ 183
            A SQ P  YR S  L  E    H  QA++ R+   R+ SP L RS + L ++QT QG  Q
Sbjct: 679  ASSQLPGSYRASSGLLGEYQNPHLQQALNPRVSQPRSPSPGLIRSPSPLLRTQTQQGAAQ 738

Query: 184  NRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPSGGN 363
              +  T    +      +             P  +VQ Q+ R ++S   N+DG R S   
Sbjct: 739  VGLGYTAGNVNSNPTRFMAAAQRATQMARQPPMVAVQTQSSR-AASYSGNVDGSRASAVE 797

Query: 364  TRVPVQPVTRPDGLVDSS---VAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQPA 534
             R+ +  +T      D+S   V+EQ+WRPTGRMRGSL+G+AYS ALSQ +  PTQ  Q A
Sbjct: 798  QRLNMGGLTPAASRADTSADLVSEQNWRPTGRMRGSLTGRAYSEALSQLMIQPTQSAQ-A 856

Query: 535  ARPLASLSTSLSAVPSPPH 591
            ARP A+L++S S    PPH
Sbjct: 857  ARPQANLTSSPSV---PPH 872