BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Acanthopanax23_contig00013242
         (981 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|KZM96144.1| hypothetical protein DCAR_019386 [Daucus carota s...   402   e-133
ref|XP_017249244.1| PREDICTED: reticulon-like protein B21 [Daucu...   402   e-133
ref|XP_022888155.1| reticulon-like protein B21 isoform X2 [Olea ...   392   e-129
ref|XP_022888156.1| reticulon-like protein B21 isoform X3 [Olea ...   388   e-128
ref|XP_022888154.1| reticulon-like protein B21 isoform X1 [Olea ...   388   e-128
emb|CDP10218.1| unnamed protein product [Coffea canephora]            384   e-125
ref|XP_019256080.1| PREDICTED: reticulon-like protein B21 [Nicot...   380   e-125
ref|XP_017248234.1| PREDICTED: reticulon-like protein B21 [Daucu...   381   e-124
ref|XP_016481746.1| PREDICTED: reticulon-like protein B21 [Nicot...   376   e-123
ref|XP_009621517.1| PREDICTED: reticulon-like protein B21 [Nicot...   376   e-123
ref|XP_016468744.1| PREDICTED: reticulon-like protein B21 [Nicot...   375   e-123
ref|XP_009760122.1| PREDICTED: reticulon-like protein B21 [Nicot...   372   e-121
gb|PIM99219.1| hypothetical protein CDL12_28289 [Handroanthus im...   362   e-121
gb|PIN03324.1| hypothetical protein CDL12_24155 [Handroanthus im...   367   e-121
ref|XP_012841125.1| PREDICTED: reticulon-like protein B21 isofor...   368   e-121
ref|XP_012841124.1| PREDICTED: reticulon-like protein B21 isofor...   368   e-121
ref|XP_010275780.1| PREDICTED: reticulon-like protein B21 isofor...   372   e-121
gb|EYU34356.1| hypothetical protein MIMGU_mgv1a004061mg [Erythra...   368   e-121
ref|XP_010275779.1| PREDICTED: reticulon-like protein B21 isofor...   372   e-121
gb|EYU34355.1| hypothetical protein MIMGU_mgv1a004061mg [Erythra...   368   e-121

>gb|KZM96144.1| hypothetical protein DCAR_019386 [Daucus carota subsp. sativus]
          Length = 612

 Score =  402 bits (1032), Expect = e-133
 Identities = 202/252 (80%), Positives = 220/252 (87%)
 Frame = +3

Query: 3    EFQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGL 182
            EF+ V   HSKLQSFVDLVMWR+ SKSAFIFGLGTFII+SSSYTTD+NISL++V+SY+GL
Sbjct: 362  EFKTVSNKHSKLQSFVDLVMWREPSKSAFIFGLGTFIIVSSSYTTDMNISLVSVISYVGL 421

Query: 183  VYLAAIFLFRSIICRGAINEDDTSQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDP 362
            VYLAAIFLFRSI+ RGA+  DDTS E+VVGEEEA+WLLKLILPYINEFL K+RALFSGDP
Sbjct: 422  VYLAAIFLFRSILHRGALIVDDTSPEYVVGEEEAIWLLKLILPYINEFLSKIRALFSGDP 481

Query: 363  ATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKD 542
            ATTMKLAVLLFVLARCGSSITIWKM KLGFFGVFTVPKICSSYSSQL AFG FWIRRFKD
Sbjct: 482  ATTMKLAVLLFVLARCGSSITIWKMVKLGFFGVFTVPKICSSYSSQLAAFGKFWIRRFKD 541

Query: 543  AWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXX 722
            AWESC+ KKGVGFF+FTVVWNLSSIVARVWAVF+LFVAFRYYQQQ               
Sbjct: 542  AWESCSQKKGVGFFVFTVVWNLSSIVARVWAVFMLFVAFRYYQQQQQ-QHDEHHSEEWIE 600

Query: 723  XXAREGSVEAKN 758
              +REGSV+ KN
Sbjct: 601  EESREGSVKVKN 612


>ref|XP_017249244.1| PREDICTED: reticulon-like protein B21 [Daucus carota subsp. sativus]
          Length = 616

 Score =  402 bits (1032), Expect = e-133
 Identities = 202/252 (80%), Positives = 220/252 (87%)
 Frame = +3

Query: 3    EFQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGL 182
            EF+ V   HSKLQSFVDLVMWR+ SKSAFIFGLGTFII+SSSYTTD+NISL++V+SY+GL
Sbjct: 366  EFKTVSNKHSKLQSFVDLVMWREPSKSAFIFGLGTFIIVSSSYTTDMNISLVSVISYVGL 425

Query: 183  VYLAAIFLFRSIICRGAINEDDTSQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDP 362
            VYLAAIFLFRSI+ RGA+  DDTS E+VVGEEEA+WLLKLILPYINEFL K+RALFSGDP
Sbjct: 426  VYLAAIFLFRSILHRGALIVDDTSPEYVVGEEEAIWLLKLILPYINEFLSKIRALFSGDP 485

Query: 363  ATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKD 542
            ATTMKLAVLLFVLARCGSSITIWKM KLGFFGVFTVPKICSSYSSQL AFG FWIRRFKD
Sbjct: 486  ATTMKLAVLLFVLARCGSSITIWKMVKLGFFGVFTVPKICSSYSSQLAAFGKFWIRRFKD 545

Query: 543  AWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXX 722
            AWESC+ KKGVGFF+FTVVWNLSSIVARVWAVF+LFVAFRYYQQQ               
Sbjct: 546  AWESCSQKKGVGFFVFTVVWNLSSIVARVWAVFMLFVAFRYYQQQQQ-QHDEHHSEEWIE 604

Query: 723  XXAREGSVEAKN 758
              +REGSV+ KN
Sbjct: 605  EESREGSVKVKN 616


>ref|XP_022888155.1| reticulon-like protein B21 isoform X2 [Olea europaea var. sylvestris]
          Length = 627

 Score =  392 bits (1006), Expect = e-129
 Identities = 201/276 (72%), Positives = 235/276 (85%), Gaps = 3/276 (1%)
 Frame = +3

Query: 3    EFQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGL 182
            +FQR P++HSKL+SFVDLVMWRDVSKSAFIFG+GTF+IISSSYT D+NIS I+V+SYLGL
Sbjct: 369  DFQRTPRSHSKLESFVDLVMWRDVSKSAFIFGIGTFVIISSSYTRDLNISFISVVSYLGL 428

Query: 183  VYLAAIFLFRSIICRGAINEDDTSQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDP 362
            VYLA+IFLFRS+I RG I   D SQ++VVGEEEA+W+LKL+LPYINEFLLKLRALFSGDP
Sbjct: 429  VYLASIFLFRSLIRRGEI---DRSQDYVVGEEEAIWVLKLVLPYINEFLLKLRALFSGDP 485

Query: 363  ATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKD 542
            +TTMKLAVLLFVLARCGSSITIWKMAKLGFFGVF VPK+CSSYS+QLTAFGTFWIRRF+D
Sbjct: 486  STTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFIVPKVCSSYSTQLTAFGTFWIRRFRD 545

Query: 543  AWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXX 722
            AWESC+HKK VGF IFT+VWNLSS+VAR+WAVF+LFVAF+YY  Q+SLM           
Sbjct: 546  AWESCSHKKAVGFAIFTLVWNLSSMVARIWAVFMLFVAFKYY--QHSLM----------- 592

Query: 723  XXAREGSVEAKNGGN---RRQGRRAPTLNEINKQKQ 821
              + E + E +N  +   +RQGRR  TL +  KQK+
Sbjct: 593  --SNEWTEEVRNEDSWQGQRQGRRT-TLTDNRKQKK 625


>ref|XP_022888156.1| reticulon-like protein B21 isoform X3 [Olea europaea var. sylvestris]
          Length = 618

 Score =  388 bits (996), Expect = e-128
 Identities = 201/277 (72%), Positives = 235/277 (84%), Gaps = 4/277 (1%)
 Frame = +3

Query: 3    EFQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGL 182
            +FQR P++HSKL+SFVDLVMWRDVSKSAFIFG+GTF+IISSSYT D+NIS I+V+SYLGL
Sbjct: 359  DFQRTPRSHSKLESFVDLVMWRDVSKSAFIFGIGTFVIISSSYTRDLNISFISVVSYLGL 418

Query: 183  VYLAAIFLFRSII-CRGAINEDDTSQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGD 359
            VYLA+IFLFRS+I  RG I   D SQ++VVGEEEA+W+LKL+LPYINEFLLKLRALFSGD
Sbjct: 419  VYLASIFLFRSLIRSRGEI---DRSQDYVVGEEEAIWVLKLVLPYINEFLLKLRALFSGD 475

Query: 360  PATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFK 539
            P+TTMKLAVLLFVLARCGSSITIWKMAKLGFFGVF VPK+CSSYS+QLTAFGTFWIRRF+
Sbjct: 476  PSTTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFIVPKVCSSYSTQLTAFGTFWIRRFR 535

Query: 540  DAWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXX 719
            DAWESC+HKK VGF IFT+VWNLSS+VAR+WAVF+LFVAF+YY  Q+SLM          
Sbjct: 536  DAWESCSHKKAVGFAIFTLVWNLSSMVARIWAVFMLFVAFKYY--QHSLM---------- 583

Query: 720  XXXAREGSVEAKNGGN---RRQGRRAPTLNEINKQKQ 821
               + E + E +N  +   +RQGRR  TL +  KQK+
Sbjct: 584  ---SNEWTEEVRNEDSWQGQRQGRRT-TLTDNRKQKK 616


>ref|XP_022888154.1| reticulon-like protein B21 isoform X1 [Olea europaea var. sylvestris]
          Length = 628

 Score =  388 bits (996), Expect = e-128
 Identities = 201/277 (72%), Positives = 235/277 (84%), Gaps = 4/277 (1%)
 Frame = +3

Query: 3    EFQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGL 182
            +FQR P++HSKL+SFVDLVMWRDVSKSAFIFG+GTF+IISSSYT D+NIS I+V+SYLGL
Sbjct: 369  DFQRTPRSHSKLESFVDLVMWRDVSKSAFIFGIGTFVIISSSYTRDLNISFISVVSYLGL 428

Query: 183  VYLAAIFLFRSII-CRGAINEDDTSQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGD 359
            VYLA+IFLFRS+I  RG I   D SQ++VVGEEEA+W+LKL+LPYINEFLLKLRALFSGD
Sbjct: 429  VYLASIFLFRSLIRSRGEI---DRSQDYVVGEEEAIWVLKLVLPYINEFLLKLRALFSGD 485

Query: 360  PATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFK 539
            P+TTMKLAVLLFVLARCGSSITIWKMAKLGFFGVF VPK+CSSYS+QLTAFGTFWIRRF+
Sbjct: 486  PSTTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFIVPKVCSSYSTQLTAFGTFWIRRFR 545

Query: 540  DAWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXX 719
            DAWESC+HKK VGF IFT+VWNLSS+VAR+WAVF+LFVAF+YY  Q+SLM          
Sbjct: 546  DAWESCSHKKAVGFAIFTLVWNLSSMVARIWAVFMLFVAFKYY--QHSLM---------- 593

Query: 720  XXXAREGSVEAKNGGN---RRQGRRAPTLNEINKQKQ 821
               + E + E +N  +   +RQGRR  TL +  KQK+
Sbjct: 594  ---SNEWTEEVRNEDSWQGQRQGRRT-TLTDNRKQKK 626


>emb|CDP10218.1| unnamed protein product [Coffea canephora]
          Length = 684

 Score =  384 bits (986), Expect = e-125
 Identities = 188/273 (68%), Positives = 225/273 (82%)
 Frame = +3

Query: 3    EFQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGL 182
            EF+  P+T SKLQS VDLVMWRD SKSAF+FG+GTFIIISSSY  D+NIS I+VLSYLGL
Sbjct: 412  EFRGSPRTRSKLQSIVDLVMWRDASKSAFVFGIGTFIIISSSYAKDLNISAISVLSYLGL 471

Query: 183  VYLAAIFLFRSIICRGAINEDDTSQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDP 362
            +YLAAIFLFRS+I RGA++ DD+S ++VVGEEEAVWL+KLILP +NEFLLK++ALFSGDP
Sbjct: 472  IYLAAIFLFRSLISRGAVDLDDSSTDYVVGEEEAVWLVKLILPCVNEFLLKIKALFSGDP 531

Query: 363  ATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKD 542
            ATTMK+A+LLFVLARCGSSITIWKM KLGFFGVFTVPK+CSSYS+QLTA+G FWIRRF+D
Sbjct: 532  ATTMKMALLLFVLARCGSSITIWKMVKLGFFGVFTVPKVCSSYSTQLTAYGVFWIRRFRD 591

Query: 543  AWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXX 722
            AWESC+HKK V F IFT+VWNLSSIVAR+WAVF+LFVA+RYYQ   S +           
Sbjct: 592  AWESCSHKKAVAFAIFTLVWNLSSIVARIWAVFMLFVAYRYYQP--SFIGEGWATAEEQT 649

Query: 723  XXAREGSVEAKNGGNRRQGRRAPTLNEINKQKQ 821
                  S   ++GG+ ++ R+ P + E  KQK+
Sbjct: 650  RAEDSSSASGQSGGHVQRQRQRPIVTETGKQKR 682


>ref|XP_019256080.1| PREDICTED: reticulon-like protein B21 [Nicotiana attenuata]
 gb|OIS97223.1| reticulon-like protein b21 [Nicotiana attenuata]
          Length = 622

 Score =  380 bits (976), Expect = e-125
 Identities = 190/273 (69%), Positives = 223/273 (81%)
 Frame = +3

Query: 3    EFQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGL 182
            EFQ +P+ HSKL+SFVDLVMWRDVSKSA IFG+GTF+IISSSYT D++IS I+VLSYLGL
Sbjct: 353  EFQNIPRQHSKLESFVDLVMWRDVSKSALIFGIGTFVIISSSYTQDLSISFISVLSYLGL 412

Query: 183  VYLAAIFLFRSIICRGAINEDDTSQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDP 362
            VYLAAIFLFRS+      N+ + S E++VGEEEA+W+LKLILPYINE LLK+RALFSGDP
Sbjct: 413  VYLAAIFLFRSLNIHRGANDINESSEYIVGEEEAMWILKLILPYINECLLKIRALFSGDP 472

Query: 363  ATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKD 542
            ATTMK+AVLLF+LARCGSSITIWKM+KLGFFGVF VPK+CSSYS+QLTA+GTFWIRRF+D
Sbjct: 473  ATTMKMAVLLFILARCGSSITIWKMSKLGFFGVFIVPKVCSSYSTQLTAYGTFWIRRFRD 532

Query: 543  AWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXX 722
            AWESCTHKK V F IFT+VWNLSSI AR+WAVF+L+V F+YYQQ+               
Sbjct: 533  AWESCTHKKAVAFAIFTLVWNLSSIAARIWAVFMLYVGFKYYQQK----LMKEGWVSEEE 588

Query: 723  XXAREGSVEAKNGGNRRQGRRAPTLNEINKQKQ 821
                E   +AK GG R+ GRR+ TL E  KQK+
Sbjct: 589  TTKAEDYWQAKIGGQRQIGRRS-TLMETRKQKK 620


>ref|XP_017248234.1| PREDICTED: reticulon-like protein B21 [Daucus carota subsp. sativus]
 gb|KZM97824.1| hypothetical protein DCAR_014814 [Daucus carota subsp. sativus]
          Length = 742

 Score =  381 bits (979), Expect = e-124
 Identities = 193/285 (67%), Positives = 221/285 (77%), Gaps = 12/285 (4%)
 Frame = +3

Query: 3    EFQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGL 182
            E+  VPK HSKLQS VDLVMWRD SKSAFIFGLGTF+I+SSSYTTD+NIS+I+V+SY+GL
Sbjct: 455  EYYSVPKRHSKLQSLVDLVMWRDASKSAFIFGLGTFVIMSSSYTTDLNISVISVVSYVGL 514

Query: 183  VYLAAIFLFRSIICRGAINEDDTSQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDP 362
            +YLA  FL RSII RG   ED+T QE+V G EEA+WLLK+ILPY+NEFLLK RALFSGDP
Sbjct: 515  IYLAITFLVRSIIQRGVTEEDETDQEYVFGVEEAIWLLKMILPYVNEFLLKARALFSGDP 574

Query: 363  ATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKD 542
            ATTMKLAVLLFVLARCGSSITIWKMAKLGFFG FTVPKICSSYSSQLTAFGTFWIRR +D
Sbjct: 575  ATTMKLAVLLFVLARCGSSITIWKMAKLGFFGAFTVPKICSSYSSQLTAFGTFWIRRIRD 634

Query: 543  AWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXX 722
            AWESC+ KK VGFF+FT++WNLSS+VAR+WAVF++FVAF+YYQQ  S             
Sbjct: 635  AWESCSQKKAVGFFLFTLIWNLSSVVARIWAVFMVFVAFKYYQQSLSDGEDWIKDDAEEE 694

Query: 723  XXAREGS----VEAKN--------GGNRRQGRRAPTLNEINKQKQ 821
               +  S    VE K         GG  R+    PT+ E  K K+
Sbjct: 695  ISGKSSSSLVLVEEKKNKSCKQIIGGRMRRQGPGPTILETKKPKK 739


>ref|XP_016481746.1| PREDICTED: reticulon-like protein B21 [Nicotiana tabacum]
          Length = 615

 Score =  376 bits (966), Expect = e-123
 Identities = 190/273 (69%), Positives = 219/273 (80%)
 Frame = +3

Query: 3    EFQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGL 182
            EF  +P+ HSKLQSFVDLVMW+D SKSA IFG+GTFIIISSSYT D+NIS I+V+SYLGL
Sbjct: 346  EFHTIPRQHSKLQSFVDLVMWKDASKSALIFGIGTFIIISSSYTQDLNISFISVVSYLGL 405

Query: 183  VYLAAIFLFRSIICRGAINEDDTSQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDP 362
            VYLAAIFLFRS+      N  + S E+VVGEEEA+W+LKLILPYINE LLK+RALFSGDP
Sbjct: 406  VYLAAIFLFRSLNIHRGANNINESSEYVVGEEEAMWILKLILPYINECLLKIRALFSGDP 465

Query: 363  ATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKD 542
            ATTMK+AVLLF+LARCGSSITIWKM+KLGFFGVF VPK+CSSYS+QLTA+GTFWIRRF+D
Sbjct: 466  ATTMKMAVLLFILARCGSSITIWKMSKLGFFGVFIVPKVCSSYSTQLTAYGTFWIRRFRD 525

Query: 543  AWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXX 722
            AWESCTHKK V F IFT+VWNLSSI AR+WAVF+L+V FRYYQQ+               
Sbjct: 526  AWESCTHKKAVAFAIFTLVWNLSSIAARIWAVFMLYVGFRYYQQK----LMKEGWVSEEE 581

Query: 723  XXAREGSVEAKNGGNRRQGRRAPTLNEINKQKQ 821
                E   + K GG R+ GRR+ TL E  KQK+
Sbjct: 582  TTKAEDYWQGKIGGQRQIGRRS-TLMESRKQKK 613


>ref|XP_009621517.1| PREDICTED: reticulon-like protein B21 [Nicotiana tomentosiformis]
          Length = 615

 Score =  376 bits (966), Expect = e-123
 Identities = 190/273 (69%), Positives = 219/273 (80%)
 Frame = +3

Query: 3    EFQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGL 182
            EF  +P+ HSKLQSFVDLVMW+D SKSA IFG+GTFIIISSSYT D+NIS I+V+SYLGL
Sbjct: 346  EFHTIPRQHSKLQSFVDLVMWKDASKSALIFGIGTFIIISSSYTQDLNISFISVVSYLGL 405

Query: 183  VYLAAIFLFRSIICRGAINEDDTSQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDP 362
            VYLAAIFLFRS+      N  + S E+VVGEEEA+W+LKLILPYINE LLK+RALFSGDP
Sbjct: 406  VYLAAIFLFRSLNIHRGANNINESSEYVVGEEEAMWILKLILPYINECLLKIRALFSGDP 465

Query: 363  ATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKD 542
            ATTMK+AVLLF+LARCGSSITIWKM+KLGFFGVF VPK+CSSYS+QLTA+GTFWIRRF+D
Sbjct: 466  ATTMKMAVLLFILARCGSSITIWKMSKLGFFGVFIVPKVCSSYSTQLTAYGTFWIRRFRD 525

Query: 543  AWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXX 722
            AWESCTHKK V F IFT+VWNLSSI AR+WAVF+L+V FRYYQQ+               
Sbjct: 526  AWESCTHKKAVAFAIFTLVWNLSSIAARIWAVFMLYVGFRYYQQK----LMKEGWVSEEE 581

Query: 723  XXAREGSVEAKNGGNRRQGRRAPTLNEINKQKQ 821
                E   + K GG R+ GRR+ TL E  KQK+
Sbjct: 582  TTKAEDYWQGKIGGQRQIGRRS-TLMESRKQKK 613


>ref|XP_016468744.1| PREDICTED: reticulon-like protein B21 [Nicotiana tabacum]
          Length = 623

 Score =  375 bits (963), Expect = e-123
 Identities = 190/273 (69%), Positives = 221/273 (80%)
 Frame = +3

Query: 3    EFQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGL 182
            EF  +P+ HSKLQSFVDLVMWRD SKSA IFG+GTF+IISSSYT D++IS I+VLSYLGL
Sbjct: 354  EFHTIPRQHSKLQSFVDLVMWRDASKSALIFGIGTFVIISSSYTQDLSISFISVLSYLGL 413

Query: 183  VYLAAIFLFRSIICRGAINEDDTSQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDP 362
            VYLAAIFLFRS+      N  + S E++VGEEEA+W+LKLILPYINE LLK+RALFSGDP
Sbjct: 414  VYLAAIFLFRSLNIHRGANNINESSEYIVGEEEAMWILKLILPYINECLLKIRALFSGDP 473

Query: 363  ATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKD 542
            ATTMK+AVLLF+LARCGSSITIWKM+KLGFFGVF VPK+CSSYS+QLTA+GTFWIRRF+D
Sbjct: 474  ATTMKMAVLLFILARCGSSITIWKMSKLGFFGVFIVPKVCSSYSTQLTAYGTFWIRRFRD 533

Query: 543  AWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXX 722
            AWESCTHKK V F IFT+VWNLSSI AR+WAVF+L+V FRYYQQ+  LM           
Sbjct: 534  AWESCTHKKAVAFAIFTLVWNLSSIAARIWAVFMLYVGFRYYQQK--LMKEGWVSEEETT 591

Query: 723  XXAREGSVEAKNGGNRRQGRRAPTLNEINKQKQ 821
                E   + K GG R+ GRR+ T+ E  KQK+
Sbjct: 592  KV--EDYWQGKIGGQRQIGRRS-TMVETRKQKK 621


>ref|XP_009760122.1| PREDICTED: reticulon-like protein B21 [Nicotiana sylvestris]
          Length = 623

 Score =  372 bits (954), Expect = e-121
 Identities = 188/273 (68%), Positives = 220/273 (80%)
 Frame = +3

Query: 3    EFQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGL 182
            E   +P+ HSKLQSFVDLVMWRD SKSA IFG+GTF+IISSSYT D++IS I+VLSYLGL
Sbjct: 354  ELHTIPRQHSKLQSFVDLVMWRDASKSALIFGIGTFVIISSSYTQDLSISFISVLSYLGL 413

Query: 183  VYLAAIFLFRSIICRGAINEDDTSQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDP 362
            VYLAAIFLFRS+      N  + S E++VGEEEA+W+LKLILPYINE L+K+RALFSGDP
Sbjct: 414  VYLAAIFLFRSLNIHRGANNINESSEYIVGEEEAMWILKLILPYINECLVKIRALFSGDP 473

Query: 363  ATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKD 542
            ATTMK+AVLLF+LARCGSSITIWKM+KLGFFGVF VPK+CSSYS+QLTA+GTFWIRRF+D
Sbjct: 474  ATTMKMAVLLFILARCGSSITIWKMSKLGFFGVFIVPKVCSSYSTQLTAYGTFWIRRFRD 533

Query: 543  AWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXX 722
            AWESCTHKK V F IFT+VWNLSSI AR+WAVF+L+V FRYYQQ+  LM           
Sbjct: 534  AWESCTHKKAVAFAIFTLVWNLSSIAARIWAVFMLYVGFRYYQQK--LMKEGWVSEEETT 591

Query: 723  XXAREGSVEAKNGGNRRQGRRAPTLNEINKQKQ 821
                E   + K GG R+ GRR+ T+ E  KQK+
Sbjct: 592  KV--EDYWQGKIGGQRQIGRRS-TMVETRKQKK 621


>gb|PIM99219.1| hypothetical protein CDL12_28289 [Handroanthus impetiginosus]
          Length = 337

 Score =  362 bits (928), Expect = e-121
 Identities = 181/273 (66%), Positives = 216/273 (79%)
 Frame = +3

Query: 3   EFQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGL 182
           +F   P T SKLQSFVDLVMWRDVSKSAFIFG+G+F IISSSY  D+NIS I+V+SYL L
Sbjct: 71  DFHGNPSTQSKLQSFVDLVMWRDVSKSAFIFGIGSFSIISSSYAKDLNISFISVVSYLSL 130

Query: 183 VYLAAIFLFRSIICRGAINEDDTSQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDP 362
           VYLAA FL +S+I RG I+ DDT Q++VVGEEEAVW++KL+LPY+NEFLL+LR LFSGDP
Sbjct: 131 VYLAANFLLKSLITRGTIDVDDTKQDYVVGEEEAVWVVKLVLPYVNEFLLRLRGLFSGDP 190

Query: 363 ATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKD 542
            TT+KLAVLLF+LARCG SIT+ KMAKLGFFG FTVPK+CSSYSSQL A+GTFWIRRF D
Sbjct: 191 PTTLKLAVLLFILARCGGSITLCKMAKLGFFGAFTVPKVCSSYSSQLNAYGTFWIRRFGD 250

Query: 543 AWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXX 722
           AWESC+HKK VGF IFT+VWN+SS+VAR+WA+FILFVAF+YYQQ                
Sbjct: 251 AWESCSHKKAVGFGIFTLVWNMSSLVARIWAMFILFVAFKYYQQ-------FSFKEGSVQ 303

Query: 723 XXAREGSVEAKNGGNRRQGRRAPTLNEINKQKQ 821
             A  G+  ++  G RR+     T+ +I KQK+
Sbjct: 304 GDAATGNANSRYIGGRRK-NPGSTIIDIKKQKK 335


>gb|PIN03324.1| hypothetical protein CDL12_24155 [Handroanthus impetiginosus]
          Length = 521

 Score =  367 bits (943), Expect = e-121
 Identities = 190/272 (69%), Positives = 220/272 (80%)
 Frame = +3

Query: 6    FQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGLV 185
            F    +T+ KLQSFVDL+MWRDVSKSAF+FG+GTF IISSSYT D+NIS I+V+SYLGLV
Sbjct: 258  FHGFSRTNIKLQSFVDLIMWRDVSKSAFVFGIGTFAIISSSYTKDLNISFISVVSYLGLV 317

Query: 186  YLAAIFLFRSIICRGAINEDDTSQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDPA 365
            YLAA FLFRS+I RG++ E    Q++VVGEEEA+W++KLILPYINEFLLKLRALFSGDPA
Sbjct: 318  YLAANFLFRSLISRGSM-ERGNDQDYVVGEEEAIWIVKLILPYINEFLLKLRALFSGDPA 376

Query: 366  TTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKDA 545
            TTMKLAVLLF+LARCGSSITIWKMAKLGF GVFTVPKICS+YSSQL A+GTFWIRRF+DA
Sbjct: 377  TTMKLAVLLFILARCGSSITIWKMAKLGFIGVFTVPKICSTYSSQLIAYGTFWIRRFRDA 436

Query: 546  WESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXXX 725
            W+SC+HKK VGF IFT+VWNLSSIVAR+WAVF+LF AF+YYQQ  SLM            
Sbjct: 437  WDSCSHKKAVGFAIFTLVWNLSSIVARIWAVFMLFAAFKYYQQ--SLM---KEGLAHDEI 491

Query: 726  XAREGSVEAKNGGNRRQGRRAPTLNEINKQKQ 821
             + + S E   G    Q     TL E+ KQK+
Sbjct: 492  GSTQNSAETIKG----QTLCRSTLTELRKQKK 519


>ref|XP_012841125.1| PREDICTED: reticulon-like protein B21 isoform X2 [Erythranthe
            guttata]
          Length = 541

 Score =  368 bits (944), Expect = e-121
 Identities = 185/273 (67%), Positives = 224/273 (82%), Gaps = 1/273 (0%)
 Frame = +3

Query: 6    FQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGLV 185
            F  V +T+SKLQSFVDLVMWRD SKSAF+FG+GTF I+SSSYT D+NIS I+V+SYLGLV
Sbjct: 274  FHGVSRTNSKLQSFVDLVMWRDASKSAFVFGVGTFAIVSSSYTKDLNISFISVISYLGLV 333

Query: 186  YLAAIFLFRSIICRGAINEDDT-SQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDP 362
            YLAAIFLFRS+I RG++  ++  +++ VVGEEEA+W +KL LPY+NEFLLKLRALFSGDP
Sbjct: 334  YLAAIFLFRSLINRGSLEMNNAKNKDFVVGEEEAIWAVKLFLPYLNEFLLKLRALFSGDP 393

Query: 363  ATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKD 542
            +TTMKLAVLLF+LARCGSSIT+WKMAKLGF GVFTVPKICS+YSSQLTA+GTFWIRRF+D
Sbjct: 394  STTMKLAVLLFILARCGSSITVWKMAKLGFIGVFTVPKICSTYSSQLTAYGTFWIRRFRD 453

Query: 543  AWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXX 722
            AWESC+HKK VGF +FT+VWNLSS+VAR+WAVF+LFVAF+YYQQ  SLM           
Sbjct: 454  AWESCSHKKAVGFALFTLVWNLSSVVARIWAVFMLFVAFKYYQQ--SLM-TREEWADEET 510

Query: 723  XXAREGSVEAKNGGNRRQGRRAPTLNEINKQKQ 821
              + + S++  N G R +     TL  +  QK+
Sbjct: 511  ISSEKSSLQGHNIGARSRS----TLTRLKSQKK 539


>ref|XP_012841124.1| PREDICTED: reticulon-like protein B21 isoform X1 [Erythranthe
            guttata]
          Length = 543

 Score =  368 bits (944), Expect = e-121
 Identities = 185/273 (67%), Positives = 224/273 (82%), Gaps = 1/273 (0%)
 Frame = +3

Query: 6    FQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGLV 185
            F  V +T+SKLQSFVDLVMWRD SKSAF+FG+GTF I+SSSYT D+NIS I+V+SYLGLV
Sbjct: 276  FHGVSRTNSKLQSFVDLVMWRDASKSAFVFGVGTFAIVSSSYTKDLNISFISVISYLGLV 335

Query: 186  YLAAIFLFRSIICRGAINEDDT-SQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDP 362
            YLAAIFLFRS+I RG++  ++  +++ VVGEEEA+W +KL LPY+NEFLLKLRALFSGDP
Sbjct: 336  YLAAIFLFRSLINRGSLEMNNAKNKDFVVGEEEAIWAVKLFLPYLNEFLLKLRALFSGDP 395

Query: 363  ATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKD 542
            +TTMKLAVLLF+LARCGSSIT+WKMAKLGF GVFTVPKICS+YSSQLTA+GTFWIRRF+D
Sbjct: 396  STTMKLAVLLFILARCGSSITVWKMAKLGFIGVFTVPKICSTYSSQLTAYGTFWIRRFRD 455

Query: 543  AWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXX 722
            AWESC+HKK VGF +FT+VWNLSS+VAR+WAVF+LFVAF+YYQQ  SLM           
Sbjct: 456  AWESCSHKKAVGFALFTLVWNLSSVVARIWAVFMLFVAFKYYQQ--SLM-TREEWADEET 512

Query: 723  XXAREGSVEAKNGGNRRQGRRAPTLNEINKQKQ 821
              + + S++  N G R +     TL  +  QK+
Sbjct: 513  ISSEKSSLQGHNIGARSRS----TLTRLKSQKK 541


>ref|XP_010275780.1| PREDICTED: reticulon-like protein B21 isoform X2 [Nelumbo nucifera]
          Length = 689

 Score =  372 bits (956), Expect = e-121
 Identities = 182/272 (66%), Positives = 221/272 (81%), Gaps = 2/272 (0%)
 Frame = +3

Query: 18   PKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGLVYLAA 197
            P+TH KLQS VDLVMWRDVS+SAF+FG+GTF ++SSS+T D+N SLI+  SYLGL+YLA 
Sbjct: 426  PETHGKLQSIVDLVMWRDVSRSAFVFGIGTFFLLSSSFTKDLNFSLISTTSYLGLIYLAL 485

Query: 198  IFLFRSIICRGAINEDDTSQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDPATTMK 377
            IF+++SI+CRGAI+ DD++Q HVVGEEEA+WL+KLILPY+NEFLLKLR LFSGDP+TTMK
Sbjct: 486  IFVYKSILCRGAIDIDDSNQTHVVGEEEAIWLIKLILPYLNEFLLKLRGLFSGDPSTTMK 545

Query: 378  LAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKDAWESC 557
            LA+LLFVLARCGSSIT+WKMAKLGFFGVFT+PKICSSYS+QLT +G FWIRRF+DAW SC
Sbjct: 546  LAILLFVLARCGSSITVWKMAKLGFFGVFTLPKICSSYSTQLTGYGKFWIRRFRDAWNSC 605

Query: 558  THKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXXXXARE 737
             HKK V F IFT+VWNLSSIVARVWAVF+L VAF+YYQQ   +                E
Sbjct: 606  AHKKAVAFGIFTLVWNLSSIVARVWAVFMLVVAFKYYQQSVIM------EDWGEEETGGE 659

Query: 738  GSV--EAKNGGNRRQGRRAPTLNEINKQKQGS 827
            GS+  + +  G ++ G   PT+ EI K+K+ S
Sbjct: 660  GSISMQEEKWGQKQGG--GPTIVEIVKEKKAS 689


>gb|EYU34356.1| hypothetical protein MIMGU_mgv1a004061mg [Erythranthe guttata]
          Length = 544

 Score =  368 bits (944), Expect = e-121
 Identities = 185/273 (67%), Positives = 224/273 (82%), Gaps = 1/273 (0%)
 Frame = +3

Query: 6    FQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGLV 185
            F  V +T+SKLQSFVDLVMWRD SKSAF+FG+GTF I+SSSYT D+NIS I+V+SYLGLV
Sbjct: 274  FHGVSRTNSKLQSFVDLVMWRDASKSAFVFGVGTFAIVSSSYTKDLNISFISVISYLGLV 333

Query: 186  YLAAIFLFRSIICRGAINEDDT-SQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDP 362
            YLAAIFLFRS+I RG++  ++  +++ VVGEEEA+W +KL LPY+NEFLLKLRALFSGDP
Sbjct: 334  YLAAIFLFRSLINRGSLEMNNAKNKDFVVGEEEAIWAVKLFLPYLNEFLLKLRALFSGDP 393

Query: 363  ATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKD 542
            +TTMKLAVLLF+LARCGSSIT+WKMAKLGF GVFTVPKICS+YSSQLTA+GTFWIRRF+D
Sbjct: 394  STTMKLAVLLFILARCGSSITVWKMAKLGFIGVFTVPKICSTYSSQLTAYGTFWIRRFRD 453

Query: 543  AWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXX 722
            AWESC+HKK VGF +FT+VWNLSS+VAR+WAVF+LFVAF+YYQQ  SLM           
Sbjct: 454  AWESCSHKKAVGFALFTLVWNLSSVVARIWAVFMLFVAFKYYQQ--SLM-TREEWADEET 510

Query: 723  XXAREGSVEAKNGGNRRQGRRAPTLNEINKQKQ 821
              + + S++  N G R +     TL  +  QK+
Sbjct: 511  ISSEKSSLQGHNIGARSRS----TLTRLKSQKK 539


>ref|XP_010275779.1| PREDICTED: reticulon-like protein B21 isoform X1 [Nelumbo nucifera]
          Length = 691

 Score =  372 bits (956), Expect = e-121
 Identities = 182/272 (66%), Positives = 221/272 (81%), Gaps = 2/272 (0%)
 Frame = +3

Query: 18   PKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGLVYLAA 197
            P+TH KLQS VDLVMWRDVS+SAF+FG+GTF ++SSS+T D+N SLI+  SYLGL+YLA 
Sbjct: 428  PETHGKLQSIVDLVMWRDVSRSAFVFGIGTFFLLSSSFTKDLNFSLISTTSYLGLIYLAL 487

Query: 198  IFLFRSIICRGAINEDDTSQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDPATTMK 377
            IF+++SI+CRGAI+ DD++Q HVVGEEEA+WL+KLILPY+NEFLLKLR LFSGDP+TTMK
Sbjct: 488  IFVYKSILCRGAIDIDDSNQTHVVGEEEAIWLIKLILPYLNEFLLKLRGLFSGDPSTTMK 547

Query: 378  LAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKDAWESC 557
            LA+LLFVLARCGSSIT+WKMAKLGFFGVFT+PKICSSYS+QLT +G FWIRRF+DAW SC
Sbjct: 548  LAILLFVLARCGSSITVWKMAKLGFFGVFTLPKICSSYSTQLTGYGKFWIRRFRDAWNSC 607

Query: 558  THKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXXXXARE 737
             HKK V F IFT+VWNLSSIVARVWAVF+L VAF+YYQQ   +                E
Sbjct: 608  AHKKAVAFGIFTLVWNLSSIVARVWAVFMLVVAFKYYQQSVIM------EDWGEEETGGE 661

Query: 738  GSV--EAKNGGNRRQGRRAPTLNEINKQKQGS 827
            GS+  + +  G ++ G   PT+ EI K+K+ S
Sbjct: 662  GSISMQEEKWGQKQGG--GPTIVEIVKEKKAS 691


>gb|EYU34355.1| hypothetical protein MIMGU_mgv1a004061mg [Erythranthe guttata]
          Length = 546

 Score =  368 bits (944), Expect = e-121
 Identities = 185/273 (67%), Positives = 224/273 (82%), Gaps = 1/273 (0%)
 Frame = +3

Query: 6    FQRVPKTHSKLQSFVDLVMWRDVSKSAFIFGLGTFIIISSSYTTDINISLITVLSYLGLV 185
            F  V +T+SKLQSFVDLVMWRD SKSAF+FG+GTF I+SSSYT D+NIS I+V+SYLGLV
Sbjct: 276  FHGVSRTNSKLQSFVDLVMWRDASKSAFVFGVGTFAIVSSSYTKDLNISFISVISYLGLV 335

Query: 186  YLAAIFLFRSIICRGAINEDDT-SQEHVVGEEEAVWLLKLILPYINEFLLKLRALFSGDP 362
            YLAAIFLFRS+I RG++  ++  +++ VVGEEEA+W +KL LPY+NEFLLKLRALFSGDP
Sbjct: 336  YLAAIFLFRSLINRGSLEMNNAKNKDFVVGEEEAIWAVKLFLPYLNEFLLKLRALFSGDP 395

Query: 363  ATTMKLAVLLFVLARCGSSITIWKMAKLGFFGVFTVPKICSSYSSQLTAFGTFWIRRFKD 542
            +TTMKLAVLLF+LARCGSSIT+WKMAKLGF GVFTVPKICS+YSSQLTA+GTFWIRRF+D
Sbjct: 396  STTMKLAVLLFILARCGSSITVWKMAKLGFIGVFTVPKICSTYSSQLTAYGTFWIRRFRD 455

Query: 543  AWESCTHKKGVGFFIFTVVWNLSSIVARVWAVFILFVAFRYYQQQYSLMXXXXXXXXXXX 722
            AWESC+HKK VGF +FT+VWNLSS+VAR+WAVF+LFVAF+YYQQ  SLM           
Sbjct: 456  AWESCSHKKAVGFALFTLVWNLSSVVARIWAVFMLFVAFKYYQQ--SLM-TREEWADEET 512

Query: 723  XXAREGSVEAKNGGNRRQGRRAPTLNEINKQKQ 821
              + + S++  N G R +     TL  +  QK+
Sbjct: 513  ISSEKSSLQGHNIGARSRS----TLTRLKSQKK 541