BLASTX nr result
ID: Acanthopanax23_contig00012663
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Acanthopanax23_contig00012663
(534 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ACJ83732.1| unknown, partial [Medicago truncatula] 166 4e-50
gb|AFK41771.1| unknown [Medicago truncatula] 168 1e-48
ref|XP_013465165.1| prohibitin complex protein [Medicago truncat... 168 1e-48
ref|XP_017248375.1| PREDICTED: prohibitin-1, mitochondrial [Dauc... 167 3e-48
gb|AFK40411.1| unknown [Lotus japonicus] 166 6e-48
ref|XP_016171151.1| prohibitin-1, mitochondrial isoform X2 [Arac... 166 1e-47
gb|AFK33601.1| unknown [Lotus japonicus] 165 2e-47
ref|XP_020229475.1| prohibitin-1, mitochondrial-like [Cajanus ca... 165 2e-47
ref|XP_016187924.1| prohibitin-1, mitochondrial [Arachis ipaensi... 165 2e-47
ref|XP_019432140.1| PREDICTED: prohibitin-1, mitochondrial-like ... 165 2e-47
gb|KHN43644.1| Prohibitin-2 [Glycine soja] >gi|947090372|gb|KRH3... 165 2e-47
ref|NP_001242309.1| uncharacterized protein LOC100806763 [Glycin... 165 2e-47
ref|XP_015899444.1| PREDICTED: prohibitin-1, mitochondrial [Zizi... 165 2e-47
ref|XP_014491666.1| prohibitin-1, mitochondrial [Vigna radiata v... 164 5e-47
ref|XP_015952856.1| prohibitin-1, mitochondrial [Arachis duranen... 164 6e-47
ref|XP_002264220.1| PREDICTED: prohibitin-1, mitochondrial isofo... 164 7e-47
ref|XP_017423143.1| PREDICTED: prohibitin-1, mitochondrial [Vign... 164 7e-47
gb|KRH22329.1| hypothetical protein GLYMA_13G293600 [Glycine max... 160 7e-47
ref|XP_003634822.1| PREDICTED: prohibitin-1, mitochondrial isofo... 164 7e-47
gb|KHN27917.1| Prohibitin-2 [Glycine soja] 165 8e-47
>gb|ACJ83732.1| unknown, partial [Medicago truncatula]
Length = 97
Score = 166 bits (419), Expect = 4e-50
Identities = 85/95 (89%), Positives = 92/95 (96%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
G+EFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSA+IRAQGEAKSA LIGQAIANNPAFI
Sbjct: 3 GKEFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAIANNPAFI 62
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQG 249
TLRKIEAAREIAHV++N+ANK YL +GDLLLN+QG
Sbjct: 63 TLRKIEAAREIAHVIANSANKVYLEAGDLLLNLQG 97
>gb|AFK41771.1| unknown [Medicago truncatula]
Length = 287
Score = 168 bits (426), Expect = 1e-48
Identities = 86/102 (84%), Positives = 96/102 (94%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
G+EFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSA+IRAQGEAKSA LIGQAIANNPAFI
Sbjct: 185 GKEFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAIANNPAFI 244
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQGVDSSSNK 228
TLRKIEAAREIAHV++N+ANK YL +GDLLLN+QG++ +K
Sbjct: 245 TLRKIEAAREIAHVIANSANKVYLEAGDLLLNLQGINLDPSK 286
>ref|XP_013465165.1| prohibitin complex protein [Medicago truncatula]
gb|KEH39200.1| prohibitin complex protein [Medicago truncatula]
Length = 287
Score = 168 bits (426), Expect = 1e-48
Identities = 86/102 (84%), Positives = 96/102 (94%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
G+EFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSA+IRAQGEAKSA LIGQAIANNPAFI
Sbjct: 185 GKEFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAIANNPAFI 244
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQGVDSSSNK 228
TLRKIEAAREIAHV++N+ANK YL +GDLLLN+QG++ +K
Sbjct: 245 TLRKIEAAREIAHVIANSANKVYLEAGDLLLNLQGINLDPSK 286
>ref|XP_017248375.1| PREDICTED: prohibitin-1, mitochondrial [Daucus carota subsp.
sativus]
gb|KZM99592.1| hypothetical protein DCAR_013046 [Daucus carota subsp. sativus]
Length = 288
Score = 167 bits (423), Expect = 3e-48
Identities = 88/102 (86%), Positives = 96/102 (94%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAI++NPAFI
Sbjct: 187 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAISSNPAFI 246
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQGVDSSSNK 228
TLRKIEAAREIA+VM++ NKTYLNSGDLLLN+ G+D + K
Sbjct: 247 TLRKIEAAREIANVMASGNNKTYLNSGDLLLNLHGMDVTPGK 288
>gb|AFK40411.1| unknown [Lotus japonicus]
Length = 289
Score = 166 bits (421), Expect = 6e-48
Identities = 86/96 (89%), Positives = 92/96 (95%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSA+IRAQGEAKSA LIGQAIANNPAFI
Sbjct: 186 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAIANNPAFI 245
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQGV 246
TLR+IEAAREIAH +SN+ANK YLNS DLLLN+QG+
Sbjct: 246 TLRRIEAAREIAHTISNSANKVYLNSDDLLLNLQGL 281
>ref|XP_016171151.1| prohibitin-1, mitochondrial isoform X2 [Arachis ipaensis]
Length = 292
Score = 166 bits (419), Expect = 1e-47
Identities = 87/102 (85%), Positives = 94/102 (92%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
G+EFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSA+IRAQGEAKSA LIGQAIANNPAFI
Sbjct: 190 GKEFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAIANNPAFI 249
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQGVDSSSNK 228
TLRKIEAAREIA+ +SNAANK YLNS DLLLN+QG+ +K
Sbjct: 250 TLRKIEAAREIANTVSNAANKVYLNSDDLLLNLQGMKLEPSK 291
>gb|AFK33601.1| unknown [Lotus japonicus]
Length = 289
Score = 165 bits (418), Expect = 2e-47
Identities = 86/96 (89%), Positives = 91/96 (94%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSA+IRAQGEAKSA LIGQAIANNPAFI
Sbjct: 186 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAIANNPAFI 245
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQGV 246
TLR+IEAAREIAH +SN ANK YLNS DLLLN+QG+
Sbjct: 246 TLRRIEAAREIAHTISNFANKVYLNSDDLLLNLQGL 281
>ref|XP_020229475.1| prohibitin-1, mitochondrial-like [Cajanus cajan]
ref|XP_020229476.1| prohibitin-1, mitochondrial-like [Cajanus cajan]
ref|XP_020229477.1| prohibitin-1, mitochondrial-like [Cajanus cajan]
gb|KYP53345.1| Prohibitin-2 [Cajanus cajan]
Length = 291
Score = 165 bits (418), Expect = 2e-47
Identities = 86/94 (91%), Positives = 90/94 (95%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
G+EFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSA+IRAQGEAKSA LIGQAIANNPAFI
Sbjct: 189 GKEFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAIANNPAFI 248
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQ 252
TLRKIEAAREIAH +SNAANK YLNS DLLLN+Q
Sbjct: 249 TLRKIEAAREIAHTISNAANKVYLNSADLLLNLQ 282
>ref|XP_016187924.1| prohibitin-1, mitochondrial [Arachis ipaensis]
ref|XP_016187925.1| prohibitin-1, mitochondrial [Arachis ipaensis]
Length = 287
Score = 165 bits (417), Expect = 2e-47
Identities = 86/94 (91%), Positives = 90/94 (95%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
G+EFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSA+IRAQGEAKSA LIGQAIANNPAFI
Sbjct: 184 GKEFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAIANNPAFI 243
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQ 252
TLRKIEAAREIAH +SNAANK YLNS DLLLN+Q
Sbjct: 244 TLRKIEAAREIAHTISNAANKVYLNSDDLLLNLQ 277
>ref|XP_019432140.1| PREDICTED: prohibitin-1, mitochondrial-like [Lupinus angustifolius]
ref|XP_019432141.1| PREDICTED: prohibitin-1, mitochondrial-like [Lupinus angustifolius]
ref|XP_019432142.1| PREDICTED: prohibitin-1, mitochondrial-like [Lupinus angustifolius]
gb|OIW21004.1| hypothetical protein TanjilG_27349 [Lupinus angustifolius]
Length = 289
Score = 165 bits (417), Expect = 2e-47
Identities = 86/94 (91%), Positives = 90/94 (95%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSA+IRAQGEAKSA LIGQAI+NNPAFI
Sbjct: 187 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAISNNPAFI 246
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQ 252
TLRKIEAAREIAH +SNAANK YLNS DLLLN+Q
Sbjct: 247 TLRKIEAAREIAHTISNAANKVYLNSDDLLLNLQ 280
>gb|KHN43644.1| Prohibitin-2 [Glycine soja]
gb|KRH39037.1| hypothetical protein GLYMA_09G173500 [Glycine max]
Length = 289
Score = 165 bits (417), Expect = 2e-47
Identities = 86/94 (91%), Positives = 90/94 (95%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
G+EFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSA+IRAQGEAKSA LIGQAIANNPAFI
Sbjct: 187 GKEFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAIANNPAFI 246
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQ 252
TLRKIEAAREIAH +SNAANK YLNS DLLLN+Q
Sbjct: 247 TLRKIEAAREIAHTISNAANKVYLNSDDLLLNLQ 280
>ref|NP_001242309.1| uncharacterized protein LOC100806763 [Glycine max]
gb|ACU21146.1| unknown [Glycine max]
Length = 289
Score = 165 bits (417), Expect = 2e-47
Identities = 86/94 (91%), Positives = 90/94 (95%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
G+EFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSA+IRAQGEAKSA LIGQAIANNPAFI
Sbjct: 187 GKEFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAIANNPAFI 246
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQ 252
TLRKIEAAREIAH +SNAANK YLNS DLLLN+Q
Sbjct: 247 TLRKIEAAREIAHTISNAANKVYLNSDDLLLNLQ 280
>ref|XP_015899444.1| PREDICTED: prohibitin-1, mitochondrial [Ziziphus jujuba]
ref|XP_015899448.1| PREDICTED: prohibitin-1, mitochondrial [Ziziphus jujuba]
ref|XP_015899456.1| PREDICTED: prohibitin-1, mitochondrial [Ziziphus jujuba]
Length = 290
Score = 165 bits (417), Expect = 2e-47
Identities = 85/97 (87%), Positives = 92/97 (94%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
GREFTAAIEAKQVAAQEAERAKFVVEKAEQDK+SAIIRAQGEA SA LIGQAIANNPAFI
Sbjct: 187 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKKSAIIRAQGEATSAQLIGQAIANNPAFI 246
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQGVD 243
LRKIEAAREIAH ++N+ANK +LNSGDLLLN+QG+D
Sbjct: 247 ALRKIEAAREIAHTIANSANKAFLNSGDLLLNLQGMD 283
>ref|XP_014491666.1| prohibitin-1, mitochondrial [Vigna radiata var. radiata]
ref|XP_014491667.1| prohibitin-1, mitochondrial [Vigna radiata var. radiata]
Length = 289
Score = 164 bits (415), Expect = 5e-47
Identities = 86/102 (84%), Positives = 93/102 (91%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
G+EFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSA+IRAQGEAKSA LIGQAIANNPAFI
Sbjct: 187 GKEFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAIANNPAFI 246
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQGVDSSSNK 228
TLRKIEAAREIAH +SNAANK +LNS DLLLN+Q + +K
Sbjct: 247 TLRKIEAAREIAHTISNAANKVFLNSDDLLLNLQEMSLDPSK 288
>ref|XP_015952856.1| prohibitin-1, mitochondrial [Arachis duranensis]
ref|XP_015952857.1| prohibitin-1, mitochondrial [Arachis duranensis]
Length = 287
Score = 164 bits (414), Expect = 6e-47
Identities = 85/94 (90%), Positives = 90/94 (95%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
G+EFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSA+IRAQGEAKSA LIGQAIANNPAFI
Sbjct: 184 GKEFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAIANNPAFI 243
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQ 252
TLRKIEAAREIAH +SN+ANK YLNS DLLLN+Q
Sbjct: 244 TLRKIEAAREIAHTISNSANKVYLNSDDLLLNLQ 277
>ref|XP_002264220.1| PREDICTED: prohibitin-1, mitochondrial isoform X1 [Vitis vinifera]
emb|CAN61836.1| hypothetical protein VITISV_018854 [Vitis vinifera]
Length = 288
Score = 164 bits (414), Expect = 7e-47
Identities = 86/100 (86%), Positives = 93/100 (93%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSA LIGQAIANNPAFI
Sbjct: 187 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSAQLIGQAIANNPAFI 246
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQGVDSSS 234
TLRKIEA+REIAH +SN+ANK +LNS DLLLN+Q ++ S
Sbjct: 247 TLRKIEASREIAHTISNSANKVFLNSNDLLLNLQEMNLQS 286
>ref|XP_017423143.1| PREDICTED: prohibitin-1, mitochondrial [Vigna angularis]
gb|KOM43750.1| hypothetical protein LR48_Vigan05g135500 [Vigna angularis]
dbj|BAT92321.1| hypothetical protein VIGAN_07101800 [Vigna angularis var.
angularis]
Length = 289
Score = 164 bits (414), Expect = 7e-47
Identities = 86/102 (84%), Positives = 93/102 (91%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
G+EFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSA+IRAQGEAKSA LIGQAIANNPAFI
Sbjct: 187 GKEFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAIANNPAFI 246
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQGVDSSSNK 228
TLRKIEAAREIAH +SNAANK +LNS DLLLN+Q + +K
Sbjct: 247 TLRKIEAAREIAHTISNAANKVFLNSDDLLLNLQEMTLDPSK 288
>gb|KRH22329.1| hypothetical protein GLYMA_13G293600 [Glycine max]
gb|KRH22330.1| hypothetical protein GLYMA_13G293600 [Glycine max]
Length = 192
Score = 160 bits (406), Expect = 7e-47
Identities = 83/102 (81%), Positives = 93/102 (91%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
G+EFTAAIEAKQVAAQEA+RAKFVVEKAEQDKRSA+IRAQGEAKSA LIGQAIANNPAFI
Sbjct: 90 GKEFTAAIEAKQVAAQEADRAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAIANNPAFI 149
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQGVDSSSNK 228
TLRKIEA REIAH +SNAANK YLNS DLLLN++ ++ + +
Sbjct: 150 TLRKIEAVREIAHTISNAANKIYLNSDDLLLNLKKMNLETGR 191
>ref|XP_003634822.1| PREDICTED: prohibitin-1, mitochondrial isoform X2 [Vitis vinifera]
Length = 290
Score = 164 bits (414), Expect = 7e-47
Identities = 86/100 (86%), Positives = 93/100 (93%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSA LIGQAIANNPAFI
Sbjct: 189 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSAQLIGQAIANNPAFI 248
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQGVDSSS 234
TLRKIEA+REIAH +SN+ANK +LNS DLLLN+Q ++ S
Sbjct: 249 TLRKIEASREIAHTISNSANKVFLNSNDLLLNLQEMNLQS 288
>gb|KHN27917.1| Prohibitin-2 [Glycine soja]
Length = 336
Score = 165 bits (417), Expect = 8e-47
Identities = 86/94 (91%), Positives = 90/94 (95%)
Frame = -2
Query: 533 GREFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAIIRAQGEAKSASLIGQAIANNPAFI 354
G+EFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSA+IRAQGEAKSA LIGQAIANNPAFI
Sbjct: 234 GKEFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAIANNPAFI 293
Query: 353 TLRKIEAAREIAHVMSNAANKTYLNSGDLLLNVQ 252
TLRKIEAAREIAH +SNAANK YLNS DLLLN+Q
Sbjct: 294 TLRKIEAAREIAHTISNAANKVYLNSDDLLLNLQ 327