BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Acanthopanax23_contig00008228
         (844 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002273666.1| PREDICTED: E3 ubiquitin-protein ligase Hakai...   131   6e-33
ref|XP_002527868.1| PREDICTED: E3 ubiquitin-protein ligase Hakai...   124   6e-31
ref|XP_015580078.1| PREDICTED: E3 ubiquitin-protein ligase Hakai...   124   6e-31
ref|XP_021651954.1| E3 ubiquitin-protein ligase Hakai-like [Heve...   127   7e-31
ref|XP_021621087.1| E3 ubiquitin-protein ligase Hakai [Manihot e...   125   5e-30
ref|XP_002308203.2| hypothetical protein POPTR_0006s09670g [Popu...   125   2e-29
dbj|GAV65496.1| hypothetical protein CFOL_v3_09011 [Cephalotus f...   120   5e-29
gb|OMO85158.1| hypothetical protein CCACVL1_10374 [Corchorus cap...   119   7e-29
ref|XP_015897113.1| PREDICTED: E3 ubiquitin-protein ligase Hakai...   124   9e-29
ref|XP_012087933.1| E3 ubiquitin-protein ligase Hakai [Jatropha ...   122   9e-29
gb|PNT30719.1| hypothetical protein POPTR_006G095600v3 [Populus ...   122   5e-28
ref|XP_018838207.1| PREDICTED: E3 ubiquitin-protein ligase Hakai...   120   5e-28
gb|OMO97438.1| hypothetical protein COLO4_14619 [Corchorus olito...   115   9e-28
gb|OVA00757.1| hypothetical protein BVC80_9083g48 [Macleaya cord...   121   2e-27
ref|XP_011019164.1| PREDICTED: E3 ubiquitin-protein ligase Hakai...   119   3e-27
ref|XP_021289334.1| E3 ubiquitin-protein ligase Hakai [Herrania ...   119   8e-27
ref|XP_007026811.2| PREDICTED: E3 ubiquitin-protein ligase Hakai...   119   8e-27
gb|EOY07313.1| RING/U-box superfamily protein, putative [Theobro...   119   8e-27
ref|XP_009786415.1| PREDICTED: E3 ubiquitin-protein ligase Hakai...   118   1e-26
ref|XP_021912208.1| E3 ubiquitin-protein ligase Hakai isoform X1...   108   4e-26

>ref|XP_002273666.1| PREDICTED: E3 ubiquitin-protein ligase Hakai [Vitis vinifera]
 ref|XP_010654211.1| PREDICTED: E3 ubiquitin-protein ligase Hakai [Vitis vinifera]
          Length = 497

 Score =  131 bits (329), Expect(2) = 6e-33
 Identities = 88/213 (41%), Positives = 106/213 (49%), Gaps = 40/213 (18%)
 Frame = -3

Query: 806 SAQISNKQKGILSETPFAEYP-MHYHQPPNVAVPNDLYSVQAPPLSCPPFAPGGGQPLYG 630
           SAQ S+KQ+GILSE+P+AEYP +H HQPPN AVP     +   PL  PP       P  G
Sbjct: 265 SAQFSDKQQGILSESPYAEYPPLHSHQPPNFAVP-----LNPNPLLTPPPFNYHHFPSEG 319

Query: 629 AQPLYGAP--MVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSGRYPQPWNTGPVDVP---- 468
           AQ  Y +P  M   D A +VG+EQGSLLGFP     G++F   YP+PWN+GPV VP    
Sbjct: 320 AQTFYSSPHEMARPDSAPDVGTEQGSLLGFPPGPVGGVNFPESYPRPWNSGPVGVPFEGP 379

Query: 467 ---------------------LFQGDSG-YPGVLPSNLPPPYSTQKGTD----------- 387
                                 FQGD G  PG LPSN P P    KG +           
Sbjct: 380 TGGQPIPDGLGNASDPQGRGAFFQGDYGRNPGSLPSNPPLPPLANKGMEPTQGSAAMDPR 439

Query: 386 DGKGTFAPESVVAATSSFTPPHISQAETREFIS 288
           DGKG  A   +        PPH+SQ +  +F S
Sbjct: 440 DGKGILAQPPLPLPPPPPPPPHLSQLKRAKFFS 472



 Score = 38.9 bits (89), Expect(2) = 6e-33
 Identities = 17/32 (53%), Positives = 20/32 (62%), Gaps = 3/32 (9%)
 Frame = -1

Query: 307 KQGNLFPRD---DGQAYPWQNEKHDGFGNGQD 221
           K+   F  D   DGQ + WQ EKHD FG+GQD
Sbjct: 466 KRAKFFSGDTSRDGQGFGWQQEKHDSFGSGQD 497


>ref|XP_002527868.1| PREDICTED: E3 ubiquitin-protein ligase Hakai isoform X1 [Ricinus
           communis]
 gb|EEF34499.1| conserved hypothetical protein [Ricinus communis]
          Length = 484

 Score =  124 bits (311), Expect(2) = 6e-31
 Identities = 83/218 (38%), Positives = 104/218 (47%), Gaps = 35/218 (16%)
 Frame = -3

Query: 836 QPGSSRGFDRSAQ---ISNKQKGILSETPFAEYP-MHYHQPPNVAVPNDLYSVQAPPLSC 669
           +PG    F +S Q      +Q+GILSETP  EYP MH  QPPN  +P +   +  P    
Sbjct: 244 RPGIQNHFQQSMQGGPPQEQQQGILSETPMPEYPAMHPIQPPNFVIPINSNPLMTPQYGL 303

Query: 668 PPFAPGGGQPLYGAQPLYGAPMVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSGRYPQPWN 489
           PPF P G QP YGA P     M   D  +E+G+EQGSLLGFP   P+G++F   Y QPWN
Sbjct: 304 PPFQPEGAQPFYGA-PYEMGQMARPDATSEIGAEQGSLLGFPPG-PAGVNFMSNYSQPWN 361

Query: 488 TGPV------------------DVPLFQGDSG-YPGVLPSNLPPPYSTQKGTD------- 387
            GP                   +   +QG+ G  PGVLP   PPP S  KG +       
Sbjct: 362 MGPAAGGQGMPDGFPNLSDSRGNAAFYQGEYGRNPGVLPMMPPPPPSANKGMEAVQGGNA 421

Query: 386 ----DGKGTFAPESVVAATSSFTP-PHISQAETREFIS 288
               D KG  AP+          P PH SQ +  ++ S
Sbjct: 422 MDARDSKGILAPQPFPHPPPPPPPLPHSSQPKRGKYYS 459



 Score = 39.3 bits (90), Expect(2) = 6e-31
 Identities = 16/34 (47%), Positives = 24/34 (70%), Gaps = 3/34 (8%)
 Frame = -1

Query: 313 KPKQGNLFPRD---DGQAYPWQNEKHDGFGNGQD 221
           +PK+G  +  D   DGQ++ WQ+E  DGFG+GQ+
Sbjct: 451 QPKRGKYYSGDMGHDGQSFGWQHENRDGFGSGQE 484


>ref|XP_015580078.1| PREDICTED: E3 ubiquitin-protein ligase Hakai isoform X2 [Ricinus
           communis]
          Length = 450

 Score =  124 bits (311), Expect(2) = 6e-31
 Identities = 83/218 (38%), Positives = 104/218 (47%), Gaps = 35/218 (16%)
 Frame = -3

Query: 836 QPGSSRGFDRSAQ---ISNKQKGILSETPFAEYP-MHYHQPPNVAVPNDLYSVQAPPLSC 669
           +PG    F +S Q      +Q+GILSETP  EYP MH  QPPN  +P +   +  P    
Sbjct: 210 RPGIQNHFQQSMQGGPPQEQQQGILSETPMPEYPAMHPIQPPNFVIPINSNPLMTPQYGL 269

Query: 668 PPFAPGGGQPLYGAQPLYGAPMVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSGRYPQPWN 489
           PPF P G QP YGA P     M   D  +E+G+EQGSLLGFP   P+G++F   Y QPWN
Sbjct: 270 PPFQPEGAQPFYGA-PYEMGQMARPDATSEIGAEQGSLLGFPPG-PAGVNFMSNYSQPWN 327

Query: 488 TGPV------------------DVPLFQGDSG-YPGVLPSNLPPPYSTQKGTD------- 387
            GP                   +   +QG+ G  PGVLP   PPP S  KG +       
Sbjct: 328 MGPAAGGQGMPDGFPNLSDSRGNAAFYQGEYGRNPGVLPMMPPPPPSANKGMEAVQGGNA 387

Query: 386 ----DGKGTFAPESVVAATSSFTP-PHISQAETREFIS 288
               D KG  AP+          P PH SQ +  ++ S
Sbjct: 388 MDARDSKGILAPQPFPHPPPPPPPLPHSSQPKRGKYYS 425



 Score = 39.3 bits (90), Expect(2) = 6e-31
 Identities = 16/34 (47%), Positives = 24/34 (70%), Gaps = 3/34 (8%)
 Frame = -1

Query: 313 KPKQGNLFPRD---DGQAYPWQNEKHDGFGNGQD 221
           +PK+G  +  D   DGQ++ WQ+E  DGFG+GQ+
Sbjct: 417 QPKRGKYYSGDMGHDGQSFGWQHENRDGFGSGQE 450


>ref|XP_021651954.1| E3 ubiquitin-protein ligase Hakai-like [Hevea brasiliensis]
 ref|XP_021651956.1| E3 ubiquitin-protein ligase Hakai-like [Hevea brasiliensis]
 ref|XP_021651957.1| E3 ubiquitin-protein ligase Hakai-like [Hevea brasiliensis]
 ref|XP_021651958.1| E3 ubiquitin-protein ligase Hakai-like [Hevea brasiliensis]
 ref|XP_021651959.1| E3 ubiquitin-protein ligase Hakai-like [Hevea brasiliensis]
          Length = 486

 Score =  127 bits (319), Expect(2) = 7e-31
 Identities = 78/179 (43%), Positives = 91/179 (50%), Gaps = 30/179 (16%)
 Frame = -3

Query: 806 SAQISNKQKGILSETPFAEYP-MHYHQPPNVAVPNDLYSVQAPPLSCPPFAPGGGQPLYG 630
           S Q S+KQ+G LSETP  EYP MH  Q PN  VP +   +  P    PPF P G QP YG
Sbjct: 258 SNQFSDKQQGNLSETPIPEYPPMHPIQSPNFVVPMNSNPLMTPQYGLPPFQPEGAQPFYG 317

Query: 629 AQPLYGAPMVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSGRYPQPWNTGPV--------- 477
           A P     M   D A EVG+EQGSLLGFP     G++F   YPQPWN+GP          
Sbjct: 318 A-PYEMGQMARPDSAPEVGAEQGSLLGFPPGPAGGVNFMSNYPQPWNSGPTSGGQAIPDG 376

Query: 476 ---------DVPLFQGDSG-YPGVLPSNLPPPYSTQKGTD----------DGKGTFAPE 360
                    +   +QGD G  PGV+P   PPP S  KG +          DGKG  AP+
Sbjct: 377 FPNHSDSRGNAAYYQGDYGRNPGVVPMIPPPPPSANKGMEAAQGGTMDSRDGKGILAPQ 435



 Score = 35.8 bits (81), Expect(2) = 7e-31
 Identities = 15/33 (45%), Positives = 21/33 (63%), Gaps = 3/33 (9%)
 Frame = -1

Query: 310 PKQGNLFPRD---DGQAYPWQNEKHDGFGNGQD 221
           PK G  +  D   +GQ + WQ+E  DGFG+GQ+
Sbjct: 454 PKGGKYYAGDMGLEGQGFGWQHENRDGFGSGQE 486


>ref|XP_021621087.1| E3 ubiquitin-protein ligase Hakai [Manihot esculenta]
 gb|OAY43655.1| hypothetical protein MANES_08G086900 [Manihot esculenta]
          Length = 488

 Score =  125 bits (313), Expect(2) = 5e-30
 Identities = 90/234 (38%), Positives = 108/234 (46%), Gaps = 51/234 (21%)
 Frame = -3

Query: 836 QPGSSR--GFDRSA----------------QISNKQKGILSETPFAEYP-MHYHQPPNVA 714
           QP ++R  GFDRS                 Q S+KQ+GILSETP  EYP MH  QP N  
Sbjct: 232 QPDNNRPPGFDRSGIPFQQSMQGGPQQESNQFSDKQQGILSETPLPEYPPMHPIQPSNFG 291

Query: 713 VPNDLYSVQAPPLSCPPFAPGGGQPLYGAQPLYGAPMVHSDYAAEVGSEQGSLLGFPLDL 534
           VP     +  P    PPF P G QP YGA P     M   D   EVG+EQGSLLGFP   
Sbjct: 292 VPMSSNPMMTPQYGLPPFQPEGAQPFYGA-PYEMGQMARPDSTPEVGAEQGSLLGFPPG- 349

Query: 533 PSGLSFSGRYPQPWNTGPV------------------DVPLFQGDSG-YPGVLPSNLPPP 411
           P+G +F   YPQPWN+G                    ++  +QGD G  PGV+P   P P
Sbjct: 350 PAGANFMANYPQPWNSGATPGGQAIPDGFPNLSDSRGNIAYYQGDYGRNPGVMPMIPPTP 409

Query: 410 YSTQKGTD----------DGKGTFAPESVVAATSSFTPP---HISQAETREFIS 288
            S  KG +          DGKG  AP+          PP   H SQ +  ++ S
Sbjct: 410 PSGNKGMEAVQGGAMDSRDGKGILAPQPFQYPPPPPPPPPMLHSSQPKRSKYYS 463



 Score = 35.4 bits (80), Expect(2) = 5e-30
 Identities = 14/34 (41%), Positives = 22/34 (64%), Gaps = 3/34 (8%)
 Frame = -1

Query: 313 KPKQGNLFPRD---DGQAYPWQNEKHDGFGNGQD 221
           +PK+   +  D   +GQ + WQ+E  DGFG+GQ+
Sbjct: 455 QPKRSKYYSSDIGHEGQGFGWQHENRDGFGSGQE 488


>ref|XP_002308203.2| hypothetical protein POPTR_0006s09670g [Populus trichocarpa]
 gb|PNT30720.1| hypothetical protein POPTR_006G095600v3 [Populus trichocarpa]
          Length = 479

 Score =  125 bits (315), Expect = 2e-29
 Identities = 81/208 (38%), Positives = 102/208 (49%), Gaps = 31/208 (14%)
 Frame = -3

Query: 818 GFDRSAQISNKQKGILSETPFAEYP-MHYHQPPNVAVPNDLYSVQAPPLSCPPFAPGGGQ 642
           GFDR  Q  NKQ+G++SETP  EYP MH  QPPN  VP +   +  P    PPF   G Q
Sbjct: 249 GFDRPGQ-QNKQQGMVSETPMPEYPPMHSIQPPNFVVPMNSNPLLTPQFGLPPFQTEGAQ 307

Query: 641 PLYGAQPLYGAPMVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSGRYPQPWNTGPV----- 477
           P Y A P     M   D A + G+EQGSLLGFP     G++F   Y QPWN+GP      
Sbjct: 308 PFYVA-PYEMGQMARPDSAPDGGAEQGSLLGFPPGPAGGMNFMANYSQPWNSGPAPGSQG 366

Query: 476 -------------DVPLFQGDSG-YPGVLPSNLPPPYSTQKGTD----------DGKGTF 369
                        ++  +QGD G  PG+LP   PPP +  KG +          DGKG  
Sbjct: 367 IPDAFANSSDSQGNIAFYQGDYGRNPGILPMVPPPPPTANKGMEAIHAGNMDHRDGKGIL 426

Query: 368 APESVVAATSSFTP-PHISQAETREFIS 288
           AP+          P PH SQ++  ++ S
Sbjct: 427 APQPFKHPPPPPPPLPHASQSKRGKYYS 454


>dbj|GAV65496.1| hypothetical protein CFOL_v3_09011 [Cephalotus follicularis]
          Length = 491

 Score =  120 bits (301), Expect(2) = 5e-29
 Identities = 86/233 (36%), Positives = 105/233 (45%), Gaps = 50/233 (21%)
 Frame = -3

Query: 836 QPGSSRGFDRSA--------QISNKQKGILSETPFAEYP-MHYHQPPNVAVPNDLYSVQA 684
           +PG    F RS         Q S+KQ+G LSE PF EYP +H  QPPN  VP +   V A
Sbjct: 240 RPGPPNSFQRSFDSLQQESNQFSDKQQGFLSENPFPEYPPLHSLQPPNFVVPMNPNPVLA 299

Query: 683 PPLSCPPFAPGGGQPLYGAQPLYGAP--MVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSG 510
           PP    PF      P  GAQP YGAP  +   D A +VGSE GSLLGFP      ++F+ 
Sbjct: 300 PPFGLAPF------PTEGAQPFYGAPYEVARQDSAPDVGSEPGSLLGFPPGSAGAMNFAA 353

Query: 509 RYPQPWNTGPV--------------------------DVPLFQGDSGYP-GVLPSNLPPP 411
            YPQPW TG                             +  +QGD G   G LP   PPP
Sbjct: 354 SYPQPWITGSAGSTHFEASLGGQPTADGFANMPDSQGKIAFYQGDYGRNLGGLPMIPPPP 413

Query: 410 YSTQKGTD-----------DGKGTFAPESV-VAATSSFTPPHISQAETREFIS 288
            S  KG D           +GKG  AP+ +         PPH+SQ +  ++ S
Sbjct: 414 PSANKGMDAVQGSNNMDHREGKGVLAPQPMQFPQPPPPPPPHLSQLKRGKYYS 466



 Score = 36.6 bits (83), Expect(2) = 5e-29
 Identities = 16/32 (50%), Positives = 20/32 (62%), Gaps = 3/32 (9%)
 Frame = -1

Query: 307 KQGNLFPRD---DGQAYPWQNEKHDGFGNGQD 221
           K+G  +  D   DGQ Y WQ+E  DGFG+ QD
Sbjct: 460 KRGKYYSGDMGRDGQGYGWQHENRDGFGSSQD 491


>gb|OMO85158.1| hypothetical protein CCACVL1_10374 [Corchorus capsularis]
          Length = 487

 Score =  119 bits (297), Expect(2) = 7e-29
 Identities = 86/216 (39%), Positives = 107/216 (49%), Gaps = 38/216 (17%)
 Frame = -3

Query: 821 RGFDR-------SAQISNKQKGILSETPFAEYP-MHYHQPPNVAVPNDLYSVQAPPLSCP 666
           +GFDR       S+Q S+KQ G+ SE  F+EYP MH  QPPN  VP +  +    P   P
Sbjct: 256 QGFDRQGTPQPESSQFSDKQPGLSSENQFSEYPPMHPMQPPNFVVPMNS-NPMLTPYGVP 314

Query: 665 PFAPGGGQPLYGAQPLYGAP--MVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSGRYPQPW 492
           PF      P  G+QP YGAP  M     A +VGSEQGSLLGFP     G+++   YPQPW
Sbjct: 315 PF------PTDGSQPFYGAPYEMARPSSAPDVGSEQGSLLGFPAGPIGGMNYPPGYPQPW 368

Query: 491 NTGPVDVPL---------------FQGDSG-YPGVLPSNLPPPYSTQKGTD--------- 387
           N G   VP                +QGD G  PG LP  +PPP S  KG +         
Sbjct: 369 NAGQPGVPFEAPPGGHMMGDAFPNYQGDYGRNPGNLPM-IPPPTSANKGMEAVQGSNAID 427

Query: 386 --DGKGTFAPESV-VAATSSFTPPHISQAETREFIS 288
             DGKG  AP+ + +       P H+SQ +  +F S
Sbjct: 428 PRDGKGILAPQPMQIPPPPPPLPHHMSQHKRGKFPS 463



 Score = 37.7 bits (86), Expect(2) = 7e-29
 Identities = 16/27 (59%), Positives = 19/27 (70%), Gaps = 3/27 (11%)
 Frame = -1

Query: 292 FPRD---DGQAYPWQNEKHDGFGNGQD 221
           FP D   DGQ + WQ+E  DGFG+GQD
Sbjct: 461 FPSDMGRDGQGFGWQHENRDGFGSGQD 487


>ref|XP_015897113.1| PREDICTED: E3 ubiquitin-protein ligase Hakai [Ziziphus jujuba]
 ref|XP_015897114.1| PREDICTED: E3 ubiquitin-protein ligase Hakai [Ziziphus jujuba]
          Length = 504

 Score =  124 bits (311), Expect(2) = 9e-29
 Identities = 82/210 (39%), Positives = 102/210 (48%), Gaps = 41/210 (19%)
 Frame = -3

Query: 800 QISNKQKGILSETPFAEYP-MHYHQPPNVAVPNDLYSVQAP--PLSCPPFAPGGGQPLYG 630
           Q S+KQ+GILS++PFAEYP MH  QPPN  VP +   +  P  P   PPF      P+ G
Sbjct: 275 QYSDKQQGILSDSPFAEYPPMHSIQPPNYMVPVNSNQMMNPSLPFGYPPF------PVDG 328

Query: 629 AQPLYGAP--MVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSGRYPQPWNTGPV------- 477
            QP YGAP  M   D A EVGSEQGSLLGFP      ++F   YPQPWN G         
Sbjct: 329 PQPYYGAPFEMARQDSAQEVGSEQGSLLGFPPGPAGNMNFPANYPQPWNAGQAGMPFEPP 388

Query: 476 ------------------DVPLFQGDSG-YPGVLPSNLPPPYSTQ----------KGTDD 384
                              VP +QG+ G  PG L  N+PPP + +              D
Sbjct: 389 QGGQGVQDGFTNTPDSQGKVPFYQGEFGRNPGGLAINIPPPSANKPMEPSPGGNAMDPRD 448

Query: 383 GKGTFAPESVVAATSSFTPPHISQAETREF 294
           GKG  AP+ +        PPH+SQ + + +
Sbjct: 449 GKGILAPQPMPLPPPPPLPPHLSQNKRKYY 478



 Score = 32.0 bits (71), Expect(2) = 9e-29
 Identities = 11/20 (55%), Positives = 14/20 (70%)
 Frame = -1

Query: 280 DGQAYPWQNEKHDGFGNGQD 221
           DGQ + WQ+E  + FG GQD
Sbjct: 485 DGQGFGWQHENRENFGTGQD 504


>ref|XP_012087933.1| E3 ubiquitin-protein ligase Hakai [Jatropha curcas]
 ref|XP_012087934.1| E3 ubiquitin-protein ligase Hakai [Jatropha curcas]
 gb|KDP24499.1| hypothetical protein JCGZ_25063 [Jatropha curcas]
          Length = 485

 Score =  122 bits (305), Expect(2) = 9e-29
 Identities = 92/237 (38%), Positives = 111/237 (46%), Gaps = 52/237 (21%)
 Frame = -3

Query: 842 ESQPGSSR--GFDR------------------SAQISNKQKGILSETPFAEYP-MHYHQP 726
           ESQP ++R  GFDR                  S Q  +KQ+GILSE P  EYP MH  QP
Sbjct: 230 ESQPDNNRPPGFDRPGLQNHFQQSMSGGTQQESNQFPDKQQGILSEGPIPEYPHMHPMQP 289

Query: 725 PNVAVPNDLYSVQAPPLSCPPFAPGGGQPLYGAQPLYGAPMVHSDYAAEVGSEQGSLLGF 546
           PN  VP +   +  P    PPF P GGQP + A P     M   D   EVG+EQGSLLGF
Sbjct: 290 PNFVVPMNSNQLITPQYGIPPFQPEGGQPFFSA-PYEMGQMARPDSTPEVGAEQGSLLGF 348

Query: 545 PLDLPSGLSFSGRYPQPWNTGPV------------------DVPLFQGDSG-YPGVLPSN 423
           P   P+G++F   YPQPWNTGP+                  +   +QGD G  PGV+   
Sbjct: 349 PPG-PAGVNFMANYPQPWNTGPMPGGQGVPDAFANLSDSRGNAAFYQGDYGRNPGVM--Q 405

Query: 422 LPPPYSTQKG-----------TDDGKGTFAPESVVAATSSFTP-PHISQAETREFIS 288
           +PP  S  KG           T DGKG  AP+          P PH SQ    ++ S
Sbjct: 406 IPP--SANKGMETVQGGNAMDTRDGKGILAPQPYGHPAPPPPPLPHSSQPTRGKYYS 460



 Score = 34.3 bits (77), Expect(2) = 9e-29
 Identities = 14/34 (41%), Positives = 21/34 (61%), Gaps = 3/34 (8%)
 Frame = -1

Query: 313 KPKQGNLFPRD---DGQAYPWQNEKHDGFGNGQD 221
           +P +G  +  D   DGQ + WQ+E  DGFG+ Q+
Sbjct: 452 QPTRGKYYSGDMGHDGQGFGWQHENRDGFGSSQE 485


>gb|PNT30719.1| hypothetical protein POPTR_006G095600v3 [Populus trichocarpa]
          Length = 498

 Score =  122 bits (306), Expect = 5e-28
 Identities = 77/204 (37%), Positives = 101/204 (49%), Gaps = 31/204 (15%)
 Frame = -3

Query: 806 SAQISNKQKGILSETPFAEYP-MHYHQPPNVAVPNDLYSVQAPPLSCPPFAPGGGQPLYG 630
           S+Q ++KQ+G++SETP  EYP MH  QPPN  VP +   +  P    PPF   G QP Y 
Sbjct: 271 SSQFADKQQGMVSETPMPEYPPMHSIQPPNFVVPMNSNPLLTPQFGLPPFQTEGAQPFYV 330

Query: 629 AQPLYGAPMVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSGRYPQPWNTGPV--------- 477
           A P     M   D A + G+EQGSLLGFP     G++F   Y QPWN+GP          
Sbjct: 331 A-PYEMGQMARPDSAPDGGAEQGSLLGFPPGPAGGMNFMANYSQPWNSGPAPGSQGIPDA 389

Query: 476 ---------DVPLFQGDSG-YPGVLPSNLPPPYSTQKGTD----------DGKGTFAPES 357
                    ++  +QGD G  PG+LP   PPP +  KG +          DGKG  AP+ 
Sbjct: 390 FANSSDSQGNIAFYQGDYGRNPGILPMVPPPPPTANKGMEAIHAGNMDHRDGKGILAPQP 449

Query: 356 VVAATSSFTP-PHISQAETREFIS 288
                    P PH SQ++  ++ S
Sbjct: 450 FKHPPPPPPPLPHASQSKRGKYYS 473


>ref|XP_018838207.1| PREDICTED: E3 ubiquitin-protein ligase Hakai [Juglans regia]
 ref|XP_018838208.1| PREDICTED: E3 ubiquitin-protein ligase Hakai [Juglans regia]
 ref|XP_018838209.1| PREDICTED: E3 ubiquitin-protein ligase Hakai [Juglans regia]
 ref|XP_018838210.1| PREDICTED: E3 ubiquitin-protein ligase Hakai [Juglans regia]
 ref|XP_018838211.1| PREDICTED: E3 ubiquitin-protein ligase Hakai [Juglans regia]
 ref|XP_018838212.1| PREDICTED: E3 ubiquitin-protein ligase Hakai [Juglans regia]
 ref|XP_018838214.1| PREDICTED: E3 ubiquitin-protein ligase Hakai [Juglans regia]
 ref|XP_018838215.1| PREDICTED: E3 ubiquitin-protein ligase Hakai [Juglans regia]
 ref|XP_018838216.1| PREDICTED: E3 ubiquitin-protein ligase Hakai [Juglans regia]
          Length = 496

 Score =  120 bits (302), Expect(2) = 5e-28
 Identities = 82/209 (39%), Positives = 102/209 (48%), Gaps = 40/209 (19%)
 Frame = -3

Query: 806 SAQISNKQKGILSETPFAEYP-MHYHQPPNVAVPNDLYSVQAPPL--SCPPFAPGGGQPL 636
           S Q S+KQ+GILSE+PF EYP MH  QP N AVP +   +  PPL    PPF P GGQP 
Sbjct: 266 SGQFSDKQQGILSESPFPEYPPMHSFQPSNFAVPVNSNPMLNPPLPYGYPPFQPEGGQPF 325

Query: 635 YGAQPLYGAPMVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSGRYPQPWNTGPVDVP---- 468
           YG        +   D A+E G+EQGSLLGFP     G++F   Y QPWN G V +P    
Sbjct: 326 YGTP----YEVTRQDSASEAGAEQGSLLGFPPVQAGGVNFPAAYAQPWNAGFVGLPFEHQ 381

Query: 467 ---------------------LFQGDSG-YPGVLPSNLPPPYSTQKGTD----------- 387
                                  QG+ G  PG +PSN PPP ++ KG +           
Sbjct: 382 PGGQGIIDGFTNASDSHGKAAFHQGEFGRSPGGMPSNPPPPLAS-KGMEQVQSGNLMDPR 440

Query: 386 DGKGTFAPESVVAATSSFTPPHISQAETR 300
           DGKG  AP+ +         PH SQ + +
Sbjct: 441 DGKGILAPQPLTLPPPPPPRPHSSQLKRK 469



 Score = 32.7 bits (73), Expect(2) = 5e-28
 Identities = 11/20 (55%), Positives = 15/20 (75%)
 Frame = -1

Query: 280 DGQAYPWQNEKHDGFGNGQD 221
           DGQ Y WQ+E  D +G+GQ+
Sbjct: 477 DGQGYGWQHENRDSYGSGQE 496


>gb|OMO97438.1| hypothetical protein COLO4_14619 [Corchorus olitorius]
          Length = 488

 Score =  115 bits (287), Expect(2) = 9e-28
 Identities = 85/216 (39%), Positives = 106/216 (49%), Gaps = 38/216 (17%)
 Frame = -3

Query: 821 RGFDR-------SAQISNKQKGILSETPFAEYP-MHYHQPPNVAVPNDLYSVQAPPLSCP 666
           +GFDR       S+Q S+KQ G+ SE  F+EYP MH  QPPN  VP +  +    P   P
Sbjct: 257 QGFDRQGTPQPESSQFSDKQPGLSSENQFSEYPPMHPMQPPNFVVPMNS-NPMLTPYGVP 315

Query: 665 PFAPGGGQPLYGAQPLYGAP--MVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSGRYPQPW 492
           PF      P  G+QP YGAP  M     A +VGSEQGSLLGFP     G+++   Y QPW
Sbjct: 316 PF------PTDGSQPFYGAPYEMARPSSAPDVGSEQGSLLGFPPGPLGGMNYPPGYSQPW 369

Query: 491 NTGPVDVPL---------------FQGDSG-YPGVLPSNLPPPYSTQKGTD--------- 387
           N G   VP                +QGD G  PG LP  +PPP S  KG +         
Sbjct: 370 NAGQPGVPFEAPPGGHMMGDAFPNYQGDYGRNPGNLPM-IPPPTSANKGMEAVQGSNAID 428

Query: 386 --DGKGTFAPESV-VAATSSFTPPHISQAETREFIS 288
             DGKG  AP+ + +       P H+SQ +  +F S
Sbjct: 429 PRDGKGILAPQPMQIPPPPPPLPHHMSQHKRGKFPS 464



 Score = 37.7 bits (86), Expect(2) = 9e-28
 Identities = 16/27 (59%), Positives = 19/27 (70%), Gaps = 3/27 (11%)
 Frame = -1

Query: 292 FPRD---DGQAYPWQNEKHDGFGNGQD 221
           FP D   DGQ + WQ+E  DGFG+GQD
Sbjct: 462 FPSDMGRDGQGFGWQHENRDGFGSGQD 488


>gb|OVA00757.1| hypothetical protein BVC80_9083g48 [Macleaya cordata]
          Length = 595

 Score =  121 bits (304), Expect = 2e-27
 Identities = 81/202 (40%), Positives = 98/202 (48%), Gaps = 31/202 (15%)
 Frame = -3

Query: 806 SAQISNKQKGILSETPFAEYPMHYHQPPNVAVPNDLYSVQAPPLSCPPFAPGGGQPLYGA 627
           S Q  +KQ+G+LSE+P ++Y MH HQ PN  VP +     AP  S  PF      P+ GA
Sbjct: 391 SDQFLDKQQGVLSESPLSDYAMHSHQSPNFVVPVNANQGLAPTFSHSPF------PVEGA 444

Query: 626 QPLYGA--PMVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSGRYPQPWNTGPVDVPL---- 465
           QP Y A       D AAE GSEQGSLLGFP  L   +SF   YP+PWN G   VPL    
Sbjct: 445 QPFYNAHFETARPDLAAEGGSEQGSLLGFPPSLGGSMSFPDNYPRPWNMGLAGVPLEQMQ 504

Query: 464 -----------------------FQGDSG-YPGVLPSNLP-PPYSTQKGTDDGKGTFAPE 360
                                  FQGD G   G LP N P PP ++     DGKG  AP+
Sbjct: 505 ASQGTGIPEGYTNLGDPQGRAQFFQGDYGRVQGGLPLNPPLPPSASAPDHKDGKGVLAPQ 564

Query: 359 SVVAATSSFTPPHISQAETREF 294
            +        PPH+SQ +  +F
Sbjct: 565 PLPPPPPP-PPPHLSQIQRGKF 585


>ref|XP_011019164.1| PREDICTED: E3 ubiquitin-protein ligase Hakai [Populus euphratica]
          Length = 498

 Score =  119 bits (299), Expect(2) = 3e-27
 Identities = 76/204 (37%), Positives = 101/204 (49%), Gaps = 31/204 (15%)
 Frame = -3

Query: 806 SAQISNKQKGILSETPFAEYP-MHYHQPPNVAVPNDLYSVQAPPLSCPPFAPGGGQPLYG 630
           S+Q ++KQ+G++SETP  EYP MH  QP N  VP +   +  P    PPF   G QP Y 
Sbjct: 271 SSQFADKQQGMVSETPMPEYPPMHSIQPSNFVVPMNSNPLLTPQFGLPPFQTEGAQPFYV 330

Query: 629 AQPLYGAPMVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSGRYPQPWNTGPV--------- 477
           A P     M   D A + G+EQGSLLGFP     G++F   Y QPWN+GP          
Sbjct: 331 A-PYEMGQMARPDSAPDGGAEQGSLLGFPPGPAGGMNFMANYSQPWNSGPAPGSQGIPDA 389

Query: 476 ---------DVPLFQGDSG-YPGVLPSNLPPPYSTQKGTD----------DGKGTFAPES 357
                    ++  +QGD G  PG+LP   PPP ++ KG +          DGKG  AP+ 
Sbjct: 390 FANSSDSQGNIAFYQGDYGRNPGILPMVPPPPPTSNKGMEAIHAGNMDHRDGKGILAPQP 449

Query: 356 VVAATSSFTP-PHISQAETREFIS 288
                    P PH SQ++  ++ S
Sbjct: 450 FKHPPPPPPPLPHASQSKRGKYYS 473



 Score = 31.2 bits (69), Expect(2) = 3e-27
 Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 3/32 (9%)
 Frame = -1

Query: 307 KQGNLFPRDDGQAYP---WQNEKHDGFGNGQD 221
           K+G  +  D G   P   WQ+E  DGFG+GQ+
Sbjct: 467 KRGKYYSGDMGHEGPDFGWQHENRDGFGSGQE 498


>ref|XP_021289334.1| E3 ubiquitin-protein ligase Hakai [Herrania umbratica]
          Length = 488

 Score =  119 bits (297), Expect = 8e-27
 Identities = 86/216 (39%), Positives = 108/216 (50%), Gaps = 38/216 (17%)
 Frame = -3

Query: 821 RGFDR-------SAQISNKQKGILSETPFAEYP-MHYHQPPNVAVPNDLYSVQAPPLSCP 666
           +GFDR       S+Q S+KQ+G+L+E  F+EYP MH  QPPN  +P +   + AP    P
Sbjct: 256 QGFDRQGTPQPESSQFSDKQQGVLAENQFSEYPPMHSMQPPNFVMPMNSNPMLAP-YGVP 314

Query: 665 PFAPGGGQPLYGAQPLYGAP--MVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSGRYPQPW 492
           PF      P  GAQP YGAP  M     A +VGSEQGSLLGFP     G+++   YPQPW
Sbjct: 315 PF------PADGAQPFYGAPYEMARPSSAPDVGSEQGSLLGFPPGPVGGINYPTGYPQPW 368

Query: 491 NTGPVDVPL---------------FQGDSG-YPGVLPSNLPPPYSTQKGTD--------- 387
           N G    P                +QGD G  PG LP  +PPP S  KG +         
Sbjct: 369 NGGHPGAPFEAPPGGHMMGDAFANYQGDYGRNPGGLPM-IPPPQSANKGMEAMQGSNSMD 427

Query: 386 --DGKGTFAPESV-VAATSSFTPPHISQAETREFIS 288
             DGKG  AP+ + V       P H+ Q +  +F S
Sbjct: 428 PRDGKGILAPQPMQVPPPPPPLPHHMPQLKRGKFHS 463


>ref|XP_007026811.2| PREDICTED: E3 ubiquitin-protein ligase Hakai [Theobroma cacao]
          Length = 488

 Score =  119 bits (297), Expect = 8e-27
 Identities = 87/216 (40%), Positives = 107/216 (49%), Gaps = 38/216 (17%)
 Frame = -3

Query: 821 RGFDR-------SAQISNKQKGILSETPFAEYP-MHYHQPPNVAVPNDLYSVQAPPLSCP 666
           +GFDR       S+Q S+KQ+G+LSE  F+EYP MH  QPPN  +P +   + AP    P
Sbjct: 256 QGFDRQGTPQPESSQFSDKQQGVLSENQFSEYPPMHSMQPPNFVMPMNSNPMFAP-YGVP 314

Query: 665 PFAPGGGQPLYGAQPLYGAP--MVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSGRYPQPW 492
           PF      P  GAQP YGAP  M     A +VGSEQGSLLGFP     G+++   YPQPW
Sbjct: 315 PF------PTDGAQPFYGAPYEMARPSSAPDVGSEQGSLLGFPPGPVGGINYPTGYPQPW 368

Query: 491 NTGPVDVPL---------------FQGDSG-YPGVLPSNLPPPYSTQKGTD--------- 387
           N G    P                +QGD G  PG LP  +PPP S  KG +         
Sbjct: 369 NGGQPGAPFEAPPGGHMMGDAFANYQGDYGRNPGGLPM-IPPPPSANKGMEAVQGSNSMD 427

Query: 386 --DGKGTFAPESV-VAATSSFTPPHISQAETREFIS 288
             DGKG  AP+ + V       P H+ Q +   F S
Sbjct: 428 PRDGKGILAPQPMQVPPPPPPLPHHMPQLKRGNFHS 463


>gb|EOY07313.1| RING/U-box superfamily protein, putative [Theobroma cacao]
          Length = 488

 Score =  119 bits (297), Expect = 8e-27
 Identities = 87/216 (40%), Positives = 107/216 (49%), Gaps = 38/216 (17%)
 Frame = -3

Query: 821 RGFDR-------SAQISNKQKGILSETPFAEYP-MHYHQPPNVAVPNDLYSVQAPPLSCP 666
           +GFDR       S+Q S+KQ+G+LSE  F+EYP MH  QPPN  +P +   + AP    P
Sbjct: 256 QGFDRQGTPQPESSQFSDKQQGVLSENQFSEYPPMHSMQPPNFVMPMNSNPMFAP-YGVP 314

Query: 665 PFAPGGGQPLYGAQPLYGAP--MVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSGRYPQPW 492
           PF      P  GAQP YGAP  M     A +VGSEQGSLLGFP     G+++   YPQPW
Sbjct: 315 PF------PTDGAQPFYGAPYEMARPSSAPDVGSEQGSLLGFPPGPVGGINYPTGYPQPW 368

Query: 491 NTGPVDVPL---------------FQGDSG-YPGVLPSNLPPPYSTQKGTD--------- 387
           N G    P                +QGD G  PG LP  +PPP S  KG +         
Sbjct: 369 NGGQPGAPFEAPPGGHMMGDAFANYQGDYGRNPGGLPM-IPPPPSANKGMEAVQGSNSMD 427

Query: 386 --DGKGTFAPESV-VAATSSFTPPHISQAETREFIS 288
             DGKG  AP+ + V       P H+ Q +   F S
Sbjct: 428 PRDGKGILAPQPMQVPPPPPPLPHHMPQLKRGNFHS 463


>ref|XP_009786415.1| PREDICTED: E3 ubiquitin-protein ligase Hakai [Nicotiana sylvestris]
 ref|XP_009786416.1| PREDICTED: E3 ubiquitin-protein ligase Hakai [Nicotiana sylvestris]
 ref|XP_016484631.1| PREDICTED: E3 ubiquitin-protein ligase Hakai-like [Nicotiana
           tabacum]
          Length = 497

 Score =  118 bits (296), Expect = 1e-26
 Identities = 74/190 (38%), Positives = 91/190 (47%), Gaps = 37/190 (19%)
 Frame = -3

Query: 836 QPGSS-----RGFDRSA-------QISNKQKGILSETPFAEYPMHYHQPPNVAVPNDLYS 693
           +PGS      +GFD          Q  +KQ+GI+  +PF EYPMH  QP   AVP     
Sbjct: 246 RPGSQNRFPQQGFDTQGALRPEPGQFPDKQQGIIGGSPFPEYPMHMSQPHGFAVPMSSNP 305

Query: 692 VQAPPLSCPPFAPGGGQPLYGAQPLYGAPMVHSDYAAEVGSEQGSLLGFPLDLPSGLSFS 513
           V AP    P FAP G QP YGA       M   D   E+GSEQGSLLGFP      ++F+
Sbjct: 306 VLAPQFGYPQFAPDGAQPFYGAP----FEMPRPDSTPEMGSEQGSLLGFPPGAAGSMNFA 361

Query: 512 GRYPQPWNTGPVDVP------------------------LFQGDSG-YPGVLPSNLPPPY 408
             YP+PWN G    P                         FQGD G   G++PSNLPPP 
Sbjct: 362 ENYPRPWNMGQQVGPFERSAVQPPGDGFVNAADPQGRPVFFQGDYGRNAGIMPSNLPPPP 421

Query: 407 STQKGTDDGK 378
           S  +G++ G+
Sbjct: 422 SANRGSEGGQ 431


>ref|XP_021912208.1| E3 ubiquitin-protein ligase Hakai isoform X1 [Carica papaya]
          Length = 506

 Score =  108 bits (271), Expect(2) = 4e-26
 Identities = 73/203 (35%), Positives = 91/203 (44%), Gaps = 30/203 (14%)
 Frame = -3

Query: 806 SAQISNKQKGILSETPFAEYPMHYHQPPNVAVPNDLYSVQAPPLSCPPFAPGGGQPLYGA 627
           S+Q+++KQ+GILSET   EYP  + QPPN  +P +   V  P    P F   G Q  Y A
Sbjct: 281 SSQLTDKQQGILSETQLPEYPPMHSQPPNYVMPTNQNPVMPPSFGLPSFQTDGVQQFYTA 340

Query: 626 QPLYGAPMVHSDYAAEVGSEQGSLLGFPLDLPSGLSFSGRYPQPWNTGPVDV-------- 471
                  M  +D   EVGS+QGSLLGFP     G+ FS +Y Q W  G V V        
Sbjct: 341 ----SYEMGRTDSTPEVGSQQGSLLGFPPGPVVGVHFSAQYQQSWTAGTVGVPFEAPMGN 396

Query: 470 -----------------PLFQGDSGYPGVLPSNLPPP-----YSTQKGTDDGKGTFAPES 357
                            P FQGD G    LP  +PPP        Q+   DGKG  A +S
Sbjct: 397 QGVQEGFANLSDSQGKAPFFQGDFGRNSGLPM-IPPPSGIKGIDVQQDNRDGKGILASQS 455

Query: 356 VVAATSSFTPPHISQAETREFIS 288
           +        PPH+S     +F S
Sbjct: 456 LQIPPPPPPPPHLSHPSRGQFYS 478



 Score = 38.5 bits (88), Expect(2) = 4e-26
 Identities = 16/33 (48%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
 Frame = -1

Query: 310 PKQGNLFPRD---DGQAYPWQNEKHDGFGNGQD 221
           P +G  +  D   DGQ+Y WQ+E HDGF   QD
Sbjct: 471 PSRGQFYSGDMGRDGQSYGWQHESHDGFAGSQD 503