BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Acanthopanax23_contig00006188
         (1140 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_017216773.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   128   1e-28
ref|XP_017216772.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   128   1e-28
gb|KZM88141.1| hypothetical protein DCAR_025216 [Daucus carota s...   118   2e-25
ref|XP_010661825.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   107   1e-21
ref|XP_010661824.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   107   1e-21
emb|CAN73361.1| hypothetical protein VITISV_006165 [Vitis vinifera]   105   7e-21
ref|XP_019071879.1| PREDICTED: E4 SUMO-protein ligase PIAL1 isof...   101   2e-19
emb|CAN82727.1| hypothetical protein VITISV_040324 [Vitis vinifera]   101   2e-19
ref|XP_010658435.1| PREDICTED: E4 SUMO-protein ligase PIAL1 isof...   101   2e-19
gb|ONI07224.1| hypothetical protein PRUPE_5G107000 [Prunus persica]    88   4e-15
ref|XP_020419685.1| E4 SUMO-protein ligase PIAL2 [Prunus persica...    88   4e-15
ref|XP_008239090.1| PREDICTED: E4 SUMO-protein ligase PIAL2 [Pru...    86   2e-14
ref|XP_002304521.1| transcription factor family protein [Populus...    83   2e-13
gb|PNT45362.1| hypothetical protein POPTR_003G133000v3 [Populus ...    83   2e-13
ref|XP_011022613.1| PREDICTED: E3 SUMO-protein ligase SIZ2-like ...    83   2e-13
ref|XP_011022612.1| PREDICTED: E3 SUMO-protein ligase SIZ2-like ...    83   2e-13
ref|XP_021820820.1| E4 SUMO-protein ligase PIAL2 isoform X2 [Pru...    81   1e-12
ref|XP_021820818.1| E4 SUMO-protein ligase PIAL2 isoform X1 [Pru...    81   1e-12
ref|XP_022737523.1| E4 SUMO-protein ligase PIAL2-like isoform X3...    80   1e-12
ref|XP_022737522.1| E4 SUMO-protein ligase PIAL2-like isoform X2...    80   1e-12

>ref|XP_017216773.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X2 [Daucus
            carota subsp. sativus]
          Length = 691

 Score =  128 bits (321), Expect = 1e-28
 Identities = 85/193 (44%), Positives = 111/193 (57%), Gaps = 2/193 (1%)
 Frame = -3

Query: 874  SVIRGIDLPTQNPHAYSSLSRPPSMHQNGLHQNPQNLNRAQTLNNSSINVHRSVCVQPVQ 695
            SV +   LP QN +AY      P++ QNG  QN ++ +  Q   NSS NV  +V VQ V 
Sbjct: 515  SVSQVNSLPVQNSNAYIL----PTVEQNGYCQNSRDTHTYQNSKNSSNNVCEAVHVQQVH 570

Query: 694  FQRDGGTRA-LSTCQQVPQISNTQPLAAAPLPAIETNSAYSLSVVTEQRQNMEGEIHLIS 518
             QRD   +A L+T  + P + +T    + P               +EQ+QNM  E + +S
Sbjct: 571  SQRDDEIQASLNTHNRQPSVLDTCSKQSRP--------------ESEQKQNMHREKNQVS 616

Query: 517  HEDGLMALASEGWRPSGRMRGSLQGRAYSEAFHQFISQPAQPTRASEPSFRLMPTLPGA- 341
            HE GL+ L SEGWRPSGRMRGSLQG+AY+EA+ +FI QP QP  ASE   RLM TL  A 
Sbjct: 617  HEGGLVELPSEGWRPSGRMRGSLQGQAYAEAYRRFIKQPTQPVGASELPSRLMATLSVAK 676

Query: 340  TQIPGIKINSLNT 302
            +Q P IK ++  T
Sbjct: 677  SQRPNIKASAQAT 689


>ref|XP_017216772.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X1 [Daucus
            carota subsp. sativus]
          Length = 694

 Score =  128 bits (321), Expect = 1e-28
 Identities = 85/193 (44%), Positives = 111/193 (57%), Gaps = 2/193 (1%)
 Frame = -3

Query: 874  SVIRGIDLPTQNPHAYSSLSRPPSMHQNGLHQNPQNLNRAQTLNNSSINVHRSVCVQPVQ 695
            SV +   LP QN +AY      P++ QNG  QN ++ +  Q   NSS NV  +V VQ V 
Sbjct: 518  SVSQVNSLPVQNSNAYIL----PTVEQNGYCQNSRDTHTYQNSKNSSNNVCEAVHVQQVH 573

Query: 694  FQRDGGTRA-LSTCQQVPQISNTQPLAAAPLPAIETNSAYSLSVVTEQRQNMEGEIHLIS 518
             QRD   +A L+T  + P + +T    + P               +EQ+QNM  E + +S
Sbjct: 574  SQRDDEIQASLNTHNRQPSVLDTCSKQSRP--------------ESEQKQNMHREKNQVS 619

Query: 517  HEDGLMALASEGWRPSGRMRGSLQGRAYSEAFHQFISQPAQPTRASEPSFRLMPTLPGA- 341
            HE GL+ L SEGWRPSGRMRGSLQG+AY+EA+ +FI QP QP  ASE   RLM TL  A 
Sbjct: 620  HEGGLVELPSEGWRPSGRMRGSLQGQAYAEAYRRFIKQPTQPVGASELPSRLMATLSVAK 679

Query: 340  TQIPGIKINSLNT 302
            +Q P IK ++  T
Sbjct: 680  SQRPNIKASAQAT 692


>gb|KZM88141.1| hypothetical protein DCAR_025216 [Daucus carota subsp. sativus]
          Length = 721

 Score =  118 bits (296), Expect = 2e-25
 Identities = 74/167 (44%), Positives = 97/167 (58%), Gaps = 1/167 (0%)
 Frame = -3

Query: 874 SVIRGIDLPTQNPHAYSSLSRPPSMHQNGLHQNPQNLNRAQTLNNSSINVHRSVCVQPVQ 695
           SV +   LP QN +AY      P++ QNG  QN ++ +  Q   NSS NV  +V VQ V 
Sbjct: 491 SVSQVNSLPVQNSNAYIL----PTVEQNGYCQNSRDTHTYQNSKNSSNNVCEAVHVQQVH 546

Query: 694 FQRDGGTRA-LSTCQQVPQISNTQPLAAAPLPAIETNSAYSLSVVTEQRQNMEGEIHLIS 518
            QRD   +A L+T  + P + +T    + P               +EQ+QNM  E + +S
Sbjct: 547 SQRDDEIQASLNTHNRQPSVLDTCSKQSRP--------------ESEQKQNMHREKNQVS 592

Query: 517 HEDGLMALASEGWRPSGRMRGSLQGRAYSEAFHQFISQPAQPTRASE 377
           HE GL+ L SEGWRPSGRMRGSLQG+AY+EA+ +FI QP QP  ASE
Sbjct: 593 HEGGLVELPSEGWRPSGRMRGSLQGQAYAEAYRRFIKQPTQPVGASE 639


>ref|XP_010661825.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X2 [Vitis vinifera]
          Length = 859

 Score =  107 bits (268), Expect = 1e-21
 Identities = 100/339 (29%), Positives = 141/339 (41%), Gaps = 28/339 (8%)
 Frame = -3

Query: 1138 SNEFKRFPSIDRQVDRSLSAIHPLP----LPLQAQ----MPCDVSAAISQIIHSLTPNIE 983
            SNE+ R  SI R + R+  A+  LP    LP  AQ    MP   ++  SQ    + P++E
Sbjct: 554  SNEYGRLASIPRHLTRNPIAVQALPAQDQLPRLAQHTRLMPTGATSTGSQTTSFMAPSVE 613

Query: 982  GCNYFLEGTELMNRXXXXXXXXXXXXXXXXXSMQNHSVIRGIDLPTQNPHAYSSLSRPPS 803
            G +     TE                        + S++    +  Q+      L  P +
Sbjct: 614  GFDAVNGVTE-------------------RDLQFSRSLMSSFPVSGQSVQRVGGLPNPRT 654

Query: 802  MHQNGLHQNPQNLNRAQTLNNSSINVHRSVCVQPVQFQ-RDGGTRALST----------- 659
                            Q +N    NVH S+ VQ +Q Q R GG++   +           
Sbjct: 655  ---------------TQAMNEPRNNVHPSIHVQSMQRQQRSGGSQVTGSVPNRQSPHAAA 699

Query: 658  CQQVPQISNTQPLAAAPLPAIETNSAYSLSVVTEQ-------RQNMEGEIHLISHEDGLM 500
             QQ  Q+S + P     L    T +A+S+ +V EQ       R+N+ G        D   
Sbjct: 700  AQQTVQVSRSPPSVPVQLRPARTGTAFSVGMVAEQLRTAGEQRRNILGTAWSTPRPDASA 759

Query: 499  ALAS-EGWRPSGRMRGSLQGRAYSEAFHQFISQPAQPTRASEPSFRLMPTLPGATQIPGI 323
            AL + E WRPSGRMRGSL G AYS A +QF+ QP QPT+A  P   L   LPG      +
Sbjct: 760  ALPTDENWRPSGRMRGSLTGEAYSAALNQFMLQPTQPTQAPLPPTSLPSNLPGGLPAFRL 819

Query: 322  KINSLNTQSIAQAQAQPSLGDATNLPGSSNSLTEQSREM 206
              +S N  + A +Q +P         GSS +L E +  M
Sbjct: 820  FPSSSNEHNAAPSQTEPQPRLRPQGGGSSGTLPEWTPRM 858


>ref|XP_010661824.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Vitis vinifera]
          Length = 883

 Score =  107 bits (268), Expect = 1e-21
 Identities = 100/339 (29%), Positives = 141/339 (41%), Gaps = 28/339 (8%)
 Frame = -3

Query: 1138 SNEFKRFPSIDRQVDRSLSAIHPLP----LPLQAQ----MPCDVSAAISQIIHSLTPNIE 983
            SNE+ R  SI R + R+  A+  LP    LP  AQ    MP   ++  SQ    + P++E
Sbjct: 578  SNEYGRLASIPRHLTRNPIAVQALPAQDQLPRLAQHTRLMPTGATSTGSQTTSFMAPSVE 637

Query: 982  GCNYFLEGTELMNRXXXXXXXXXXXXXXXXXSMQNHSVIRGIDLPTQNPHAYSSLSRPPS 803
            G +     TE                        + S++    +  Q+      L  P +
Sbjct: 638  GFDAVNGVTE-------------------RDLQFSRSLMSSFPVSGQSVQRVGGLPNPRT 678

Query: 802  MHQNGLHQNPQNLNRAQTLNNSSINVHRSVCVQPVQFQ-RDGGTRALST----------- 659
                            Q +N    NVH S+ VQ +Q Q R GG++   +           
Sbjct: 679  ---------------TQAMNEPRNNVHPSIHVQSMQRQQRSGGSQVTGSVPNRQSPHAAA 723

Query: 658  CQQVPQISNTQPLAAAPLPAIETNSAYSLSVVTEQ-------RQNMEGEIHLISHEDGLM 500
             QQ  Q+S + P     L    T +A+S+ +V EQ       R+N+ G        D   
Sbjct: 724  AQQTVQVSRSPPSVPVQLRPARTGTAFSVGMVAEQLRTAGEQRRNILGTAWSTPRPDASA 783

Query: 499  ALAS-EGWRPSGRMRGSLQGRAYSEAFHQFISQPAQPTRASEPSFRLMPTLPGATQIPGI 323
            AL + E WRPSGRMRGSL G AYS A +QF+ QP QPT+A  P   L   LPG      +
Sbjct: 784  ALPTDENWRPSGRMRGSLTGEAYSAALNQFMLQPTQPTQAPLPPTSLPSNLPGGLPAFRL 843

Query: 322  KINSLNTQSIAQAQAQPSLGDATNLPGSSNSLTEQSREM 206
              +S N  + A +Q +P         GSS +L E +  M
Sbjct: 844  FPSSSNEHNAAPSQTEPQPRLRPQGGGSSGTLPEWTPRM 882


>emb|CAN73361.1| hypothetical protein VITISV_006165 [Vitis vinifera]
          Length = 845

 Score =  105 bits (262), Expect = 7e-21
 Identities = 98/333 (29%), Positives = 137/333 (41%), Gaps = 28/333 (8%)
 Frame = -3

Query: 1138 SNEFKRFPSIDRQVDRSLSAIHPLP----LPLQAQ----MPCDVSAAISQIIHSLTPNIE 983
            SNE+ R  SI R + R+  A+  LP    LP  AQ    MP   ++  SQ    + P++E
Sbjct: 537  SNEYGRLASIPRHLTRNPIAVQALPAQDQLPRLAQHTRLMPTGATSTGSQTTSFMAPSVE 596

Query: 982  GCNYFLEGTELMNRXXXXXXXXXXXXXXXXXSMQNHSVIRGIDLPTQNPHAYSSLSRPPS 803
            G +     TE                        + S++    +  Q+      L  P +
Sbjct: 597  GFDAVNGVTE-------------------RDLQFSRSLMSSFPVSGQSVQRVGGLPNPRT 637

Query: 802  MHQNGLHQNPQNLNRAQTLNNSSINVHRSVCVQPVQFQ-RDGGTRALST----------- 659
                            Q +N    NVH S+ VQ +Q Q R GG++   +           
Sbjct: 638  ---------------TQAMNEPRNNVHPSIHVQSMQRQQRSGGSQVTGSVPNRQSPHAAA 682

Query: 658  CQQVPQISNTQPLAAAPLPAIETNSAYSLSVVTEQ-------RQNMEGEIHLISHEDGLM 500
             QQ  Q+S + P     L    T +A+S+ +V EQ       R+N+ G        D   
Sbjct: 683  AQQTVQVSRSPPSVPVQLRPARTGTAFSVGMVAEQLRTAGEQRRNILGTAWSTPRPDASA 742

Query: 499  ALAS-EGWRPSGRMRGSLQGRAYSEAFHQFISQPAQPTRASEPSFRLMPTLPGATQIPGI 323
            AL + E WRPSGRMRGSL G AYS A +QF+ QP QPT+A  P   L   LPG      +
Sbjct: 743  ALPTDENWRPSGRMRGSLTGEAYSAALNQFMLQPTQPTQAPLPPTSLPSNLPGGLPAFRL 802

Query: 322  KINSLNTQSIAQAQAQPSLGDATNLPGSSNSLT 224
              +S N  + A +Q +P         GSS   T
Sbjct: 803  FPSSSNEHNAAPSQTEPQPRLRPQGGGSSRDFT 835


>ref|XP_019071879.1| PREDICTED: E4 SUMO-protein ligase PIAL1 isoform X2 [Vitis vinifera]
          Length = 898

 Score =  101 bits (251), Expect = 2e-19
 Identities = 102/339 (30%), Positives = 147/339 (43%), Gaps = 31/339 (9%)
 Frame = -3

Query: 1138 SNEFKRFPSIDRQVDRSLSAIHPLPLPLQAQMPCDVSAAISQIIHSLTPNIEGCNYFLEG 959
            SNE+ RFP+I R + R+  A+  LP   Q   P   S      + S+ PN  G N     
Sbjct: 569  SNEYGRFPTIPRHITRTPIAVQALPAQTQTSGPHHRSRTT---LISMVPN--GPNTVGSD 623

Query: 958  TELMNRXXXXXXXXXXXXXXXXXSMQNHSV-------IRGIDLPTQNPH--AYSSLSRPP 806
             E   +                 ++Q+HS+       + G    +Q P   AY + S  P
Sbjct: 624  MERPQQFSRSIFNPVQISDISASALQHHSMSQNWNQQVAGHPTTSQRPGPGAYRTSSGLP 683

Query: 805  SMHQN-GLHQNPQNLNRAQTLNNSSINVHRSVCVQPVQFQRDGGTRALSTCQQVPQIS-N 632
            +  Q     Q+PQ    +  L +S+ +  RS   Q       GG +  +T      IS N
Sbjct: 684  TEPQTLQQQQSPQARTHSNLLRSSAHHHSRSQVQQ-------GGAQGRATHAVGTGISQN 736

Query: 631  TQPLAAA---------PLPA----IETNSAYSL------SVVTEQRQNMEGEIHLISHED 509
             QP+ AA         PLP       T SA+ +      S   EQR N+EG +  +S  +
Sbjct: 737  AQPMVAAQRAAQMTRMPLPVQNQTSRTGSAFPVNANGGRSTAGEQRGNIEGMVQAVSRPE 796

Query: 508  GLMALASE-GWRPSGRMRGSLQGRAYSEAFHQFISQPAQPTRASEPSFRLMPTLPGATQI 332
             L+ LASE  WRP+G MRGSL GRAY+ A +Q + QP QPT+++ P   +    PG    
Sbjct: 797  SLVDLASEQNWRPTGLMRGSLVGRAYNSALNQLVIQPTQPTQSTRPPTPITSPPPGFPPH 856

Query: 331  PGIKINSLNTQSIAQAQAQPSLGDATNLPGSSNSLTEQS 215
                + ++ T  + QA   P    A+   G S  L E+S
Sbjct: 857  LQALLTNIRTPLVPQAPNYPMTQPAST-TGGSGILPERS 894


>emb|CAN82727.1| hypothetical protein VITISV_040324 [Vitis vinifera]
          Length = 901

 Score =  101 bits (251), Expect = 2e-19
 Identities = 102/339 (30%), Positives = 147/339 (43%), Gaps = 31/339 (9%)
 Frame = -3

Query: 1138 SNEFKRFPSIDRQVDRSLSAIHPLPLPLQAQMPCDVSAAISQIIHSLTPNIEGCNYFLEG 959
            SNE+ RFP+I R + R+  A+  LP   Q   P   S      + S+ PN  G N     
Sbjct: 572  SNEYGRFPTIPRHITRTPIAVQALPAQTQTSGPHHRSRTT---LISMVPN--GPNTVGSD 626

Query: 958  TELMNRXXXXXXXXXXXXXXXXXSMQNHSV-------IRGIDLPTQNPH--AYSSLSRPP 806
             E   +                 ++Q+HS+       + G    +Q P   AY + S  P
Sbjct: 627  MERPQQFSRSIFNPVQISDISASALQHHSMSQNWNQQVAGHPTTSQRPGPGAYRTSSGLP 686

Query: 805  SMHQN-GLHQNPQNLNRAQTLNNSSINVHRSVCVQPVQFQRDGGTRALSTCQQVPQIS-N 632
            +  Q     Q+PQ    +  L +S+ +  RS   Q       GG +  +T      IS N
Sbjct: 687  TEPQTLQQQQSPQARTHSNLLRSSAHHHSRSQVQQ-------GGAQGRATHAVGTGISQN 739

Query: 631  TQPLAAA---------PLPA----IETNSAYSL------SVVTEQRQNMEGEIHLISHED 509
             QP+ AA         PLP       T SA+ +      S   EQR N+EG +  +S  +
Sbjct: 740  AQPMVAAQRAAQMTRMPLPVQNQTSRTGSAFPVNANGGRSTAGEQRGNIEGMVQAVSRPE 799

Query: 508  GLMALASE-GWRPSGRMRGSLQGRAYSEAFHQFISQPAQPTRASEPSFRLMPTLPGATQI 332
             L+ LASE  WRP+G MRGSL GRAY+ A +Q + QP QPT+++ P   +    PG    
Sbjct: 800  SLVDLASEQNWRPTGLMRGSLVGRAYNSALNQLVIQPTQPTQSTRPPTPITSPPPGFPPH 859

Query: 331  PGIKINSLNTQSIAQAQAQPSLGDATNLPGSSNSLTEQS 215
                + ++ T  + QA   P    A+   G S  L E+S
Sbjct: 860  LQALLTNIRTPLVPQAPNYPMTQPAST-TGGSGILPERS 897


>ref|XP_010658435.1| PREDICTED: E4 SUMO-protein ligase PIAL1 isoform X1 [Vitis vinifera]
          Length = 922

 Score =  101 bits (251), Expect = 2e-19
 Identities = 102/339 (30%), Positives = 147/339 (43%), Gaps = 31/339 (9%)
 Frame = -3

Query: 1138 SNEFKRFPSIDRQVDRSLSAIHPLPLPLQAQMPCDVSAAISQIIHSLTPNIEGCNYFLEG 959
            SNE+ RFP+I R + R+  A+  LP   Q   P   S      + S+ PN  G N     
Sbjct: 593  SNEYGRFPTIPRHITRTPIAVQALPAQTQTSGPHHRSRTT---LISMVPN--GPNTVGSD 647

Query: 958  TELMNRXXXXXXXXXXXXXXXXXSMQNHSV-------IRGIDLPTQNPH--AYSSLSRPP 806
             E   +                 ++Q+HS+       + G    +Q P   AY + S  P
Sbjct: 648  MERPQQFSRSIFNPVQISDISASALQHHSMSQNWNQQVAGHPTTSQRPGPGAYRTSSGLP 707

Query: 805  SMHQN-GLHQNPQNLNRAQTLNNSSINVHRSVCVQPVQFQRDGGTRALSTCQQVPQIS-N 632
            +  Q     Q+PQ    +  L +S+ +  RS   Q       GG +  +T      IS N
Sbjct: 708  TEPQTLQQQQSPQARTHSNLLRSSAHHHSRSQVQQ-------GGAQGRATHAVGTGISQN 760

Query: 631  TQPLAAA---------PLPA----IETNSAYSL------SVVTEQRQNMEGEIHLISHED 509
             QP+ AA         PLP       T SA+ +      S   EQR N+EG +  +S  +
Sbjct: 761  AQPMVAAQRAAQMTRMPLPVQNQTSRTGSAFPVNANGGRSTAGEQRGNIEGMVQAVSRPE 820

Query: 508  GLMALASE-GWRPSGRMRGSLQGRAYSEAFHQFISQPAQPTRASEPSFRLMPTLPGATQI 332
             L+ LASE  WRP+G MRGSL GRAY+ A +Q + QP QPT+++ P   +    PG    
Sbjct: 821  SLVDLASEQNWRPTGLMRGSLVGRAYNSALNQLVIQPTQPTQSTRPPTPITSPPPGFPPH 880

Query: 331  PGIKINSLNTQSIAQAQAQPSLGDATNLPGSSNSLTEQS 215
                + ++ T  + QA   P    A+   G S  L E+S
Sbjct: 881  LQALLTNIRTPLVPQAPNYPMTQPAST-TGGSGILPERS 918


>gb|ONI07224.1| hypothetical protein PRUPE_5G107000 [Prunus persica]
          Length = 854

 Score = 88.2 bits (217), Expect = 4e-15
 Identities = 89/322 (27%), Positives = 130/322 (40%), Gaps = 42/322 (13%)
 Frame = -3

Query: 1138 SNEFKRFPSIDRQVDRSLSAIHPLPLPLQ-------------AQMPCDVSAAISQIIHSL 998
            +NE+ RF S  R + R+ +A+  LP   Q             +  P   S + SQ+  S+
Sbjct: 527  NNEYGRFAS-HRVLPRTPTAVQALPAQSQGPGLQQRPRTSWNSSTPSSASLS-SQVGQSI 584

Query: 997  TPNIEGCNYFLEGTELMNRXXXXXXXXXXXXXXXXXSMQN------------------HS 872
            TP   G N      E                     S+Q+                   S
Sbjct: 585  TPTANGVNAVCSDLERQQHFSRPRMNPLQVSNIASSSLQHPSQTTQNWDRQDQSFIHGQS 644

Query: 871  VIRGIDLPTQNPHAYSSLSRPPSMHQNGLH--QNPQNLNRAQTLNNSSINVHRSVCVQPV 698
            V + + LP  +    ++ + P  M     H  Q   N    QT+  SS ++  S  +   
Sbjct: 645  VQQVVGLPVPSQLQSANRASPGLMDFQNAHLQQAFNNARTPQTMGQSSSSIRSSSHLSRA 704

Query: 697  QFQRDGGTRALSTCQQVPQISNTQPLAAAP-LPAIETNSAYSLSVVTEQRQ-------NM 542
              Q+  G   + T Q    ++N Q   A   L AI    + S+ V  +  +       N+
Sbjct: 705  HIQQ--GNAQVGTGQTSSSLNNQQRFKAGTHLAAIMARQSPSMPVQNQTPRTRPSLPVNV 762

Query: 541  EGEIHLISHEDGLMALASE-GWRPSGRMRGSLQGRAYSEAFHQFISQPAQPTRASEPSFR 365
             G +  +S  DG + L+SE  WRP+GRMRGSL GRAYS AFHQFI  P QPT+A+ P   
Sbjct: 763  GGTMQAVSGADGSVDLSSEQNWRPTGRMRGSLSGRAYSAAFHQFIIAPTQPTQAARPPPN 822

Query: 364  LMPTLPGATQIPGIKINSLNTQ 299
            L    P     P   I +++ Q
Sbjct: 823  LTSPPPVVPSQPETLIGNMSAQ 844


>ref|XP_020419685.1| E4 SUMO-protein ligase PIAL2 [Prunus persica]
 gb|ONI07225.1| hypothetical protein PRUPE_5G107000 [Prunus persica]
          Length = 893

 Score = 88.2 bits (217), Expect = 4e-15
 Identities = 89/322 (27%), Positives = 130/322 (40%), Gaps = 42/322 (13%)
 Frame = -3

Query: 1138 SNEFKRFPSIDRQVDRSLSAIHPLPLPLQ-------------AQMPCDVSAAISQIIHSL 998
            +NE+ RF S  R + R+ +A+  LP   Q             +  P   S + SQ+  S+
Sbjct: 566  NNEYGRFAS-HRVLPRTPTAVQALPAQSQGPGLQQRPRTSWNSSTPSSASLS-SQVGQSI 623

Query: 997  TPNIEGCNYFLEGTELMNRXXXXXXXXXXXXXXXXXSMQN------------------HS 872
            TP   G N      E                     S+Q+                   S
Sbjct: 624  TPTANGVNAVCSDLERQQHFSRPRMNPLQVSNIASSSLQHPSQTTQNWDRQDQSFIHGQS 683

Query: 871  VIRGIDLPTQNPHAYSSLSRPPSMHQNGLH--QNPQNLNRAQTLNNSSINVHRSVCVQPV 698
            V + + LP  +    ++ + P  M     H  Q   N    QT+  SS ++  S  +   
Sbjct: 684  VQQVVGLPVPSQLQSANRASPGLMDFQNAHLQQAFNNARTPQTMGQSSSSIRSSSHLSRA 743

Query: 697  QFQRDGGTRALSTCQQVPQISNTQPLAAAP-LPAIETNSAYSLSVVTEQRQ-------NM 542
              Q+  G   + T Q    ++N Q   A   L AI    + S+ V  +  +       N+
Sbjct: 744  HIQQ--GNAQVGTGQTSSSLNNQQRFKAGTHLAAIMARQSPSMPVQNQTPRTRPSLPVNV 801

Query: 541  EGEIHLISHEDGLMALASE-GWRPSGRMRGSLQGRAYSEAFHQFISQPAQPTRASEPSFR 365
             G +  +S  DG + L+SE  WRP+GRMRGSL GRAYS AFHQFI  P QPT+A+ P   
Sbjct: 802  GGTMQAVSGADGSVDLSSEQNWRPTGRMRGSLSGRAYSAAFHQFIIAPTQPTQAARPPPN 861

Query: 364  LMPTLPGATQIPGIKINSLNTQ 299
            L    P     P   I +++ Q
Sbjct: 862  LTSPPPVVPSQPETLIGNMSAQ 883


>ref|XP_008239090.1| PREDICTED: E4 SUMO-protein ligase PIAL2 [Prunus mume]
          Length = 844

 Score = 85.9 bits (211), Expect = 2e-14
 Identities = 64/204 (31%), Positives = 97/204 (47%), Gaps = 9/204 (4%)
 Frame = -3

Query: 883  QNHSVIRGIDLPTQNPHAYSSLSRPPSMHQNGLHQNPQNLNRAQTLNNSSINVHRSVCVQ 704
            Q+   + G+ +P+Q   A  +           L Q   N    QT+  SS ++  S  + 
Sbjct: 633  QSVQQVVGLPVPSQLQSANRASPGLMDFQNAHLQQAFNNARTPQTMGQSSSSIRSSSHLS 692

Query: 703  PVQFQRDGGTRALSTCQQVPQISNTQP-LAAAPLPAIETNSAYSLSVVTEQRQ------- 548
                Q+  G   + T Q    ++N Q  +A A L A+    + S+ V  + ++       
Sbjct: 693  RAHIQQ--GNAQVGTGQTSSSLNNQQRFMAGAHLAAMMARQSPSMPVQNQTQRTRPSLPV 750

Query: 547  NMEGEIHLISHEDGLMALASE-GWRPSGRMRGSLQGRAYSEAFHQFISQPAQPTRASEPS 371
            N+ G +  +S  DG + L+SE  WRP+GRMRGSL GRAYS AF+QFI  P QPT+A+ P 
Sbjct: 751  NVGGTMQAVSGADGSVDLSSEQNWRPTGRMRGSLSGRAYSAAFNQFIIAPTQPTQAARPP 810

Query: 370  FRLMPTLPGATQIPGIKINSLNTQ 299
              L    P     P   I +++ Q
Sbjct: 811  PNLTSPPPVVPSQPQTLIGNMSAQ 834


>ref|XP_002304521.1| transcription factor family protein [Populus trichocarpa]
 gb|PNT45363.1| hypothetical protein POPTR_003G133000v3 [Populus trichocarpa]
          Length = 822

 Score = 83.2 bits (204), Expect = 2e-13
 Identities = 74/246 (30%), Positives = 112/246 (45%), Gaps = 29/246 (11%)
 Frame = -3

Query: 886  MQNHSVIRG-------IDLPTQNPHAYSSLSRPPSMHQNGLHQNPQNLNRAQTLNNSSIN 728
            MQ+H  + G       + LP+ +  A +  +  P++    L+Q P  L   Q+ ++S   
Sbjct: 582  MQDHPFMHGQSAQQQAVTLPSSSQLAGAHRASSPNL----LYQQP--LRVPQSRSHSPNV 635

Query: 727  VHRSVCVQPVQFQRDGGTRALSTCQQVPQISNTQPLAAAPLPA------------IETNS 584
            V  S+ + P Q Q+      +           ++ + AA L A            I+T+ 
Sbjct: 636  VRSSLPLAPAQTQQGAAQVGVGNSAGATNSQQSRLMVAAQLAAQRARQPPSVPVQIQTSG 695

Query: 583  A---YSLSV------VTEQRQNMEGEIHLISHEDGLMALASE-GWRPSGRMRGSLQGRAY 434
            A   Y  S        TEQR N  G +  +S  +GL+ LASE  WRP+GRMRGSL GRAY
Sbjct: 696  AGASYLTSADGIRAPATEQRGNAGGALPAVSGTEGLVDLASEQNWRPTGRMRGSLSGRAY 755

Query: 433  SEAFHQFISQPAQPTRASEPSFRLMPTLPGATQIPGIKINSLNTQSIAQAQAQPSLGDAT 254
            S A  +F+ QP Q T+   P   L P+   ++  P ++        + QAQ+ P  G A 
Sbjct: 756  SAALKEFMVQPTQQTQTPRPPPNLPPS--QSSMPPHLQFLFARNAQVPQAQSSPVTGSAI 813

Query: 253  NLPGSS 236
            +   SS
Sbjct: 814  SNGSSS 819


>gb|PNT45362.1| hypothetical protein POPTR_003G133000v3 [Populus trichocarpa]
          Length = 913

 Score = 83.2 bits (204), Expect = 2e-13
 Identities = 74/246 (30%), Positives = 112/246 (45%), Gaps = 29/246 (11%)
 Frame = -3

Query: 886  MQNHSVIRG-------IDLPTQNPHAYSSLSRPPSMHQNGLHQNPQNLNRAQTLNNSSIN 728
            MQ+H  + G       + LP+ +  A +  +  P++    L+Q P  L   Q+ ++S   
Sbjct: 673  MQDHPFMHGQSAQQQAVTLPSSSQLAGAHRASSPNL----LYQQP--LRVPQSRSHSPNV 726

Query: 727  VHRSVCVQPVQFQRDGGTRALSTCQQVPQISNTQPLAAAPLPA------------IETNS 584
            V  S+ + P Q Q+      +           ++ + AA L A            I+T+ 
Sbjct: 727  VRSSLPLAPAQTQQGAAQVGVGNSAGATNSQQSRLMVAAQLAAQRARQPPSVPVQIQTSG 786

Query: 583  A---YSLSV------VTEQRQNMEGEIHLISHEDGLMALASE-GWRPSGRMRGSLQGRAY 434
            A   Y  S        TEQR N  G +  +S  +GL+ LASE  WRP+GRMRGSL GRAY
Sbjct: 787  AGASYLTSADGIRAPATEQRGNAGGALPAVSGTEGLVDLASEQNWRPTGRMRGSLSGRAY 846

Query: 433  SEAFHQFISQPAQPTRASEPSFRLMPTLPGATQIPGIKINSLNTQSIAQAQAQPSLGDAT 254
            S A  +F+ QP Q T+   P   L P+   ++  P ++        + QAQ+ P  G A 
Sbjct: 847  SAALKEFMVQPTQQTQTPRPPPNLPPS--QSSMPPHLQFLFARNAQVPQAQSSPVTGSAI 904

Query: 253  NLPGSS 236
            +   SS
Sbjct: 905  SNGSSS 910


>ref|XP_011022613.1| PREDICTED: E3 SUMO-protein ligase SIZ2-like isoform X2 [Populus
            euphratica]
          Length = 910

 Score = 82.8 bits (203), Expect = 2e-13
 Identities = 69/241 (28%), Positives = 112/241 (46%), Gaps = 21/241 (8%)
 Frame = -3

Query: 886  MQNHSVIRG-------IDLPTQNPHAYSSLSRPPSMHQNGLHQNPQNLNRAQTLNNSSIN 728
            +Q+H  + G       + LP+ +  A +  +  P++      + PQ+ + +  +  SS+ 
Sbjct: 672  LQDHPFMHGQSAQQQAVTLPSSSQLAGAHRASSPNLLYQQPLRVPQSRSHSPNVVRSSLP 731

Query: 727  VHRSVCVQ-PVQFQRDGG------TRALSTCQQVPQISNTQPLAAAPLPAIETNSAYSLS 569
            +  ++  Q   Q     G      +R +   Q V Q +   P     +      ++Y  S
Sbjct: 732  LAPALTQQGAAQVGNSAGATNSQQSRLMVAAQLVAQRARQPPSVPVQIQTSGAGASYLTS 791

Query: 568  V------VTEQRQNMEGEIHLISHEDGLMALASE-GWRPSGRMRGSLQGRAYSEAFHQFI 410
                    TEQR N  G +  +S  +GL+ LASE  WRP+GRMRGSL GRAYS A  +F+
Sbjct: 792  ADGIRAPATEQRGNAGGALPAVSGTEGLVDLASEQNWRPTGRMRGSLSGRAYSAALKEFM 851

Query: 409  SQPAQPTRASEPSFRLMPTLPGATQIPGIKINSLNTQSIAQAQAQPSLGDATNLPGSSNS 230
             QP Q T+   P   L P+   ++  P ++        + QAQ+ P  G A +  GSS++
Sbjct: 852  VQPTQQTQTPRPPPNLPPS--QSSMPPHLQFLFARNAQVPQAQSSPVSGSAIS-NGSSST 908

Query: 229  L 227
            L
Sbjct: 909  L 909


>ref|XP_011022612.1| PREDICTED: E3 SUMO-protein ligase SIZ2-like isoform X1 [Populus
            euphratica]
          Length = 916

 Score = 82.8 bits (203), Expect = 2e-13
 Identities = 69/241 (28%), Positives = 112/241 (46%), Gaps = 21/241 (8%)
 Frame = -3

Query: 886  MQNHSVIRG-------IDLPTQNPHAYSSLSRPPSMHQNGLHQNPQNLNRAQTLNNSSIN 728
            +Q+H  + G       + LP+ +  A +  +  P++      + PQ+ + +  +  SS+ 
Sbjct: 678  LQDHPFMHGQSAQQQAVTLPSSSQLAGAHRASSPNLLYQQPLRVPQSRSHSPNVVRSSLP 737

Query: 727  VHRSVCVQ-PVQFQRDGG------TRALSTCQQVPQISNTQPLAAAPLPAIETNSAYSLS 569
            +  ++  Q   Q     G      +R +   Q V Q +   P     +      ++Y  S
Sbjct: 738  LAPALTQQGAAQVGNSAGATNSQQSRLMVAAQLVAQRARQPPSVPVQIQTSGAGASYLTS 797

Query: 568  V------VTEQRQNMEGEIHLISHEDGLMALASE-GWRPSGRMRGSLQGRAYSEAFHQFI 410
                    TEQR N  G +  +S  +GL+ LASE  WRP+GRMRGSL GRAYS A  +F+
Sbjct: 798  ADGIRAPATEQRGNAGGALPAVSGTEGLVDLASEQNWRPTGRMRGSLSGRAYSAALKEFM 857

Query: 409  SQPAQPTRASEPSFRLMPTLPGATQIPGIKINSLNTQSIAQAQAQPSLGDATNLPGSSNS 230
             QP Q T+   P   L P+   ++  P ++        + QAQ+ P  G A +  GSS++
Sbjct: 858  VQPTQQTQTPRPPPNLPPS--QSSMPPHLQFLFARNAQVPQAQSSPVSGSAIS-NGSSST 914

Query: 229  L 227
            L
Sbjct: 915  L 915


>ref|XP_021820820.1| E4 SUMO-protein ligase PIAL2 isoform X2 [Prunus avium]
          Length = 868

 Score = 80.9 bits (198), Expect = 1e-12
 Identities = 89/320 (27%), Positives = 129/320 (40%), Gaps = 40/320 (12%)
 Frame = -3

Query: 1138 SNEFKRFPSIDRQVDRSLSAIHPLPLPLQA----QMP-------CDVSAAIS-QIIHSLT 995
            +NE+ RF S  R + R+ +A+  LP   Q     Q P          SA +S Q+  S+T
Sbjct: 542  NNEYGRFAS-HRVLPRTPTAVQALPAQSQGPGLQQRPRTSWNSSTPSSAPLSSQVGQSIT 600

Query: 994  PNIEGCNYFLEGTELMNRXXXXXXXXXXXXXXXXXSM-----------QNHSVIRG---- 860
            P   G N      E                                  Q+ S I G    
Sbjct: 601  PTANGVNAVCSDLERQQHFSRPRMNPLVSNIASSSQQHPSQTTQNWDRQDQSFIHGQSVQ 660

Query: 859  --IDLPTQNPHAYSSLSRPPSMHQNGLH--QNPQNLNRAQTLNNSSINVHRSVCVQPVQF 692
              + LP  +    ++ + P  M  N  H  Q   N    QT+  SS ++  S  +     
Sbjct: 661  QVVGLPVPSQLQSANRASPGLMDFNNAHLQQAFNNARTPQTMGQSSSSIRSSSHLSRAHI 720

Query: 691  QRDGGTRALSTCQQVPQISNTQP-LAAAPLPAIETNSAYSLSVVTEQRQ-------NMEG 536
            Q   G   + T Q    ++N Q  +  A   A+    + S+ V  +  +       N+ G
Sbjct: 721  QP--GIAQVGTGQTSSSLNNQQRFMPGAHRAAMIARQSPSMPVQNQTPRTRPSLPVNIGG 778

Query: 535  EIHLISHEDGLMALASE-GWRPSGRMRGSLQGRAYSEAFHQFISQPAQPTRASEPSFRLM 359
             +  +S  DG + L+SE  WRP+GRMRGSL GRAYS AF+QFI  P QPT+A+ P   L 
Sbjct: 779  AMEAVSGADGSVDLSSEQNWRPTGRMRGSLSGRAYSAAFNQFIIAPTQPTQAARPPPNLT 838

Query: 358  PTLPGATQIPGIKINSLNTQ 299
               P     P   I +++ Q
Sbjct: 839  SPPPVVLSQPQTLIGNMSAQ 858


>ref|XP_021820818.1| E4 SUMO-protein ligase PIAL2 isoform X1 [Prunus avium]
          Length = 892

 Score = 80.9 bits (198), Expect = 1e-12
 Identities = 89/320 (27%), Positives = 129/320 (40%), Gaps = 40/320 (12%)
 Frame = -3

Query: 1138 SNEFKRFPSIDRQVDRSLSAIHPLPLPLQA----QMP-------CDVSAAIS-QIIHSLT 995
            +NE+ RF S  R + R+ +A+  LP   Q     Q P          SA +S Q+  S+T
Sbjct: 566  NNEYGRFAS-HRVLPRTPTAVQALPAQSQGPGLQQRPRTSWNSSTPSSAPLSSQVGQSIT 624

Query: 994  PNIEGCNYFLEGTELMNRXXXXXXXXXXXXXXXXXSM-----------QNHSVIRG---- 860
            P   G N      E                                  Q+ S I G    
Sbjct: 625  PTANGVNAVCSDLERQQHFSRPRMNPLVSNIASSSQQHPSQTTQNWDRQDQSFIHGQSVQ 684

Query: 859  --IDLPTQNPHAYSSLSRPPSMHQNGLH--QNPQNLNRAQTLNNSSINVHRSVCVQPVQF 692
              + LP  +    ++ + P  M  N  H  Q   N    QT+  SS ++  S  +     
Sbjct: 685  QVVGLPVPSQLQSANRASPGLMDFNNAHLQQAFNNARTPQTMGQSSSSIRSSSHLSRAHI 744

Query: 691  QRDGGTRALSTCQQVPQISNTQP-LAAAPLPAIETNSAYSLSVVTEQRQ-------NMEG 536
            Q   G   + T Q    ++N Q  +  A   A+    + S+ V  +  +       N+ G
Sbjct: 745  QP--GIAQVGTGQTSSSLNNQQRFMPGAHRAAMIARQSPSMPVQNQTPRTRPSLPVNIGG 802

Query: 535  EIHLISHEDGLMALASE-GWRPSGRMRGSLQGRAYSEAFHQFISQPAQPTRASEPSFRLM 359
             +  +S  DG + L+SE  WRP+GRMRGSL GRAYS AF+QFI  P QPT+A+ P   L 
Sbjct: 803  AMEAVSGADGSVDLSSEQNWRPTGRMRGSLSGRAYSAAFNQFIIAPTQPTQAARPPPNLT 862

Query: 358  PTLPGATQIPGIKINSLNTQ 299
               P     P   I +++ Q
Sbjct: 863  SPPPVVLSQPQTLIGNMSAQ 882


>ref|XP_022737523.1| E4 SUMO-protein ligase PIAL2-like isoform X3 [Durio zibethinus]
          Length = 893

 Score = 80.5 bits (197), Expect = 1e-12
 Identities = 72/224 (32%), Positives = 101/224 (45%), Gaps = 10/224 (4%)
 Frame = -3

Query: 844  QNPHAYSSLS---RPPSMHQNGLHQNPQNLNRAQTLNNSSINVHRSVCVQPVQFQRDGGT 674
            QNPH   +L+     P     GL ++P  L R QT   ++  V        V       T
Sbjct: 677  QNPHLQQALNPRVSQPRSPSPGLIRSPSPLLRTQTQQGAA-QVGLGYTAGNVN---SNPT 732

Query: 673  RALSTCQQVPQISNTQPLAAAPLPAIETNSAYSLSV------VTEQRQNMEGEIHLISHE 512
            R ++  Q+  Q++   P+ A    +    S YS +V        EQR NM G     S  
Sbjct: 733  RFMAAAQRATQMARQPPMVAVQTQSSRAAS-YSGNVDGSRASAVEQRLNMGGLTPAASRA 791

Query: 511  DGLMALASE-GWRPSGRMRGSLQGRAYSEAFHQFISQPAQPTRASEPSFRLMPTLPGATQ 335
            D    L SE  WRP+GRMRGSL GRAYSEA  Q + QP Q  +A+ P   L  +      
Sbjct: 792  DTSADLVSEQNWRPTGRMRGSLTGRAYSEALSQLMIQPTQSAQAARPQANLTSSPSVPPH 851

Query: 334  IPGIKINSLNTQSIAQAQAQPSLGDATNLPGSSNSLTEQSREMR 203
            +  +  NS N  ++ Q Q   ++ +   + GSS  L ++S  MR
Sbjct: 852  LQALLANSRN-PAVPQMQ-NNAITETAGMNGSSVVLPDRSGGMR 893


>ref|XP_022737522.1| E4 SUMO-protein ligase PIAL2-like isoform X2 [Durio zibethinus]
          Length = 914

 Score = 80.5 bits (197), Expect = 1e-12
 Identities = 72/224 (32%), Positives = 101/224 (45%), Gaps = 10/224 (4%)
 Frame = -3

Query: 844  QNPHAYSSLS---RPPSMHQNGLHQNPQNLNRAQTLNNSSINVHRSVCVQPVQFQRDGGT 674
            QNPH   +L+     P     GL ++P  L R QT   ++  V        V       T
Sbjct: 698  QNPHLQQALNPRVSQPRSPSPGLIRSPSPLLRTQTQQGAA-QVGLGYTAGNVN---SNPT 753

Query: 673  RALSTCQQVPQISNTQPLAAAPLPAIETNSAYSLSV------VTEQRQNMEGEIHLISHE 512
            R ++  Q+  Q++   P+ A    +    S YS +V        EQR NM G     S  
Sbjct: 754  RFMAAAQRATQMARQPPMVAVQTQSSRAAS-YSGNVDGSRASAVEQRLNMGGLTPAASRA 812

Query: 511  DGLMALASE-GWRPSGRMRGSLQGRAYSEAFHQFISQPAQPTRASEPSFRLMPTLPGATQ 335
            D    L SE  WRP+GRMRGSL GRAYSEA  Q + QP Q  +A+ P   L  +      
Sbjct: 813  DTSADLVSEQNWRPTGRMRGSLTGRAYSEALSQLMIQPTQSAQAARPQANLTSSPSVPPH 872

Query: 334  IPGIKINSLNTQSIAQAQAQPSLGDATNLPGSSNSLTEQSREMR 203
            +  +  NS N  ++ Q Q   ++ +   + GSS  L ++S  MR
Sbjct: 873  LQALLANSRN-PAVPQMQ-NNAITETAGMNGSSVVLPDRSGGMR 914