BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Acanthopanax23_contig00003321
         (1268 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_017252679.1| PREDICTED: low-temperature-induced cysteine ...   580   0.0  
ref|XP_017247051.1| PREDICTED: low-temperature-induced cysteine ...   579   0.0  
emb|CDP07460.1| unnamed protein product [Coffea canephora]            565   0.0  
ref|XP_016490385.1| PREDICTED: zingipain-2-like [Nicotiana tabacum]   560   0.0  
ref|XP_009597081.1| PREDICTED: zingipain-2 [Nicotiana tomentosif...   560   0.0  
ref|XP_019244955.1| PREDICTED: zingipain-2 [Nicotiana attenuata]...   555   0.0  
ref|XP_010271530.1| PREDICTED: low-temperature-induced cysteine ...   553   0.0  
ref|XP_009771293.1| PREDICTED: zingipain-2 [Nicotiana sylvestris...   552   0.0  
ref|XP_004152671.1| PREDICTED: zingipain-2 [Cucumis sativus] >gi...   549   0.0  
ref|XP_022769083.1| low-temperature-induced cysteine proteinase ...   549   0.0  
ref|XP_008444761.1| PREDICTED: zingipain-2 [Cucumis melo]             548   0.0  
ref|XP_012071947.1| low-temperature-induced cysteine proteinase ...   546   0.0  
gb|EEF52646.1| cysteine protease, putative [Ricinus communis]         545   0.0  
ref|XP_023759345.1| low-temperature-induced cysteine proteinase ...   543   0.0  
ref|XP_022862440.1| zingipain-2 [Olea europaea var. sylvestris]       543   0.0  
ref|XP_002510459.2| PREDICTED: low-temperature-induced cysteine ...   545   0.0  
gb|PON90985.1| Cyseine protease [Trema orientalis]                    544   0.0  
ref|XP_016568491.1| PREDICTED: low-temperature-induced cysteine ...   543   0.0  
ref|XP_023759344.1| low-temperature-induced cysteine proteinase ...   543   0.0  
ref|XP_024030266.1| ervatamin-B isoform X2 [Morus notabilis]          542   0.0  

>ref|XP_017252679.1| PREDICTED: low-temperature-induced cysteine proteinase-like [Daucus
            carota subsp. sativus]
          Length = 445

 Score =  580 bits (1494), Expect = 0.0
 Identities = 286/397 (72%), Positives = 317/397 (79%), Gaps = 7/397 (1%)
 Frame = +1

Query: 97   FLGLTLLRSVPLLCSSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAYIIHHNN- 273
            FL  TLL  +P + SS  TSDLF++WC+ HGKTY S+QEKL RLK+FEENY YI  HNN 
Sbjct: 7    FLVPTLLLCIPCIYSSP-TSDLFEAWCISHGKTYSSQQEKLHRLKIFEENYFYITQHNNN 65

Query: 274  ---MANSSYILSL---NAFADLTPHEFKASRLGLSAAANGLTRMNRGSSVQESSGSRDIP 435
                ANSS++ +L   NAFADLT  EFKASRLGLSA  NGL RMN G S + S G  ++P
Sbjct: 66   HIMAANSSFLYTLSVDNAFADLTHQEFKASRLGLSA--NGLIRMNLGGSSKGSDGVANVP 123

Query: 436  SSLDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRSYNS 615
            +SLDWR KGAVTNVKDQGSCGACWSFSATGA+EGINQIVTGSLTSLSEQELVDCDRSYN+
Sbjct: 124  TSLDWRDKGAVTNVKDQGSCGACWSFSATGAIEGINQIVTGSLTSLSEQELVDCDRSYNN 183

Query: 616  GCGGGLMDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPENDEKQ 795
            GC GGLMDY YQFV+KNKGIDTE+DYPYQ+ + +C+KNKLK+HVVTIDGYIDV ENDEK+
Sbjct: 184  GCEGGLMDYAYQFVIKNKGIDTEDDYPYQSRDTTCNKNKLKKHVVTIDGYIDVRENDEKE 243

Query: 796  LLQAVAAQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIVKNS 975
            LL AVAAQPVSVGICGSER FQLYSKGIF GPCST LDHAVLIVGYGSENGVDYWIVKNS
Sbjct: 244  LLAAVAAQPVSVGICGSERNFQLYSKGIFNGPCSTSLDHAVLIVGYGSENGVDYWIVKNS 303

Query: 976  WGKQWGMDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXXXXX 1155
            WGKQWGM+GYM+MQRNSGNS+GICGINMMASY                 KCSLL      
Sbjct: 304  WGKQWGMNGYMHMQRNSGNSQGICGINMMASYPTKTSPNPPPSPSPGPAKCSLLTSCSEG 363

Query: 1156 XXXXXXWRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
                    L G+CLSWKCCE+N+AVCC D+ HCCP D
Sbjct: 364  ETCCCARTLFGICLSWKCCELNAAVCCDDHRHCCPQD 400


>ref|XP_017247051.1| PREDICTED: low-temperature-induced cysteine proteinase-like [Daucus
            carota subsp. sativus]
 gb|KZM98801.1| hypothetical protein DCAR_013837 [Daucus carota subsp. sativus]
          Length = 448

 Score =  579 bits (1492), Expect = 0.0
 Identities = 294/406 (72%), Positives = 320/406 (78%), Gaps = 10/406 (2%)
 Frame = +1

Query: 79   MSWVCLFLGLTLLRSVPL-LCSSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAY 255
            M+ +   L   LL  +P  + SSS T DLF +WC+ HGKTY SEQEKL R KVFEENYAY
Sbjct: 1    MNQLYSLLFFVLLLHIPCCIHSSSTTYDLFQTWCVTHGKTYASEQEKLSRFKVFEENYAY 60

Query: 256  IIHHNN--MANSS---YILSLNAFADLTPHEFKASRLGLSAAANG-LTRMNRGSS-VQES 414
            I  HNN   ANSS   Y LSLNAFADLT  EFKASRLGLSA  N  L RM RGSS V+ S
Sbjct: 61   ITQHNNDLAANSSSSLYTLSLNAFADLTNQEFKASRLGLSAGGNDKLIRMKRGSSIVKGS 120

Query: 415  SGSR--DIPSSLDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQEL 588
            SG R  +IPSSLDWR KGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQEL
Sbjct: 121  SGVRKAEIPSSLDWRDKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQEL 180

Query: 589  VDCDRSYNSGCGGGLMDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYI 768
            VDCDRSYN GC GGLMDY YQFV+ NKGIDTE+DYPY++ E +CDK+KL RHVVTIDGY+
Sbjct: 181  VDCDRSYNLGCDGGLMDYAYQFVIDNKGIDTEDDYPYKSRETTCDKDKLNRHVVTIDGYV 240

Query: 769  DVPENDEKQLLQAVAAQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENG 948
            DV ENDE QLL+AVAAQPVSVGICGSER FQLYSKGIF+GPCSTYLDHAVLIVGYGSE+G
Sbjct: 241  DVRENDEAQLLEAVAAQPVSVGICGSERDFQLYSKGIFSGPCSTYLDHAVLIVGYGSEDG 300

Query: 949  VDYWIVKNSWGKQWGMDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKC 1128
            VDYWI+KNSWGKQWGMDGYMYMQRN+GNS+GICGINMMASY                 KC
Sbjct: 301  VDYWILKNSWGKQWGMDGYMYMQRNNGNSQGICGINMMASYPTKTSPNPPPSPSPVPTKC 360

Query: 1129 SLLXXXXXXXXXXXXWRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
            SLL            W LLG CL+WKCC ++SAVCC D+ HCCP+D
Sbjct: 361  SLLYSCSEGETCCCSWSLLGFCLTWKCCGLDSAVCCDDHKHCCPND 406


>emb|CDP07460.1| unnamed protein product [Coffea canephora]
          Length = 441

 Score =  565 bits (1457), Expect = 0.0
 Identities = 268/399 (67%), Positives = 314/399 (78%), Gaps = 3/399 (0%)
 Frame = +1

Query: 79   MSWVCLFLGLTLLRSVPLLCSSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAYI 258
            MSW   +L + LL   P +C SS T+DLF++WC  HGKTY SE+EK +RL+VFE+NY Y+
Sbjct: 1    MSWFWSYLTVVLLVFHPPICKSSLTADLFENWCKQHGKTYPSEEEKQYRLRVFEDNYDYV 60

Query: 259  IHHNNMANSSYILSLNAFADLTPHEFKASRLGLSAAANGLTRMNRGSSVQESSGS---RD 429
              HN++ANS+Y LSLNAFADLT HEFKA  LG SA+A+GL R+NRGSS   +SG+    D
Sbjct: 61   TKHNSLANSTYTLSLNAFADLTHHEFKAKYLGFSASADGLIRLNRGSSSIGASGAVGKYD 120

Query: 430  IPSSLDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRSY 609
            IPSSLDWR KGAVTNVKDQGSCGACW+FSATGA+EGIN+IVTGSL SLSEQEL+DCDRSY
Sbjct: 121  IPSSLDWRNKGAVTNVKDQGSCGACWAFSATGAIEGINEIVTGSLVSLSEQELIDCDRSY 180

Query: 610  NSGCGGGLMDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPENDE 789
            N+GC GGLMDY Y+FVVKN GIDTE+DYP++  + +C+ NKLKR VV+IDGYIDVP N+E
Sbjct: 181  NNGCNGGLMDYTYEFVVKNGGIDTEQDYPFKGRDGTCNSNKLKRRVVSIDGYIDVPANNE 240

Query: 790  KQLLQAVAAQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIVK 969
            ++LLQAVAAQPVSVGICGSERGFQLYS GIFTGPCST LDHAVLIVGY S+NG DYWIVK
Sbjct: 241  QELLQAVAAQPVSVGICGSERGFQLYSGGIFTGPCSTSLDHAVLIVGYDSKNGADYWIVK 300

Query: 970  NSWGKQWGMDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXXX 1149
            NSWG  WG++GY+++ RNSGNS G+CGINMMASY                 KCSL     
Sbjct: 301  NSWGTSWGINGYIHIIRNSGNSAGVCGINMMASYPTKSSLNPPPSPPPGPTKCSLFSSCP 360

Query: 1150 XXXXXXXXWRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
                       LG+CLSWKCC+++SAVCCKD  HCCPHD
Sbjct: 361  AGETCCCSMEFLGLCLSWKCCDLDSAVCCKDRLHCCPHD 399


>ref|XP_016490385.1| PREDICTED: zingipain-2-like [Nicotiana tabacum]
          Length = 439

 Score =  560 bits (1443), Expect = 0.0
 Identities = 268/399 (67%), Positives = 308/399 (77%), Gaps = 3/399 (0%)
 Frame = +1

Query: 79   MSWVCLFLGLTLLRSVPLLCSSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAYI 258
            MSW+C  L L LL     +C+ S  SDLF+SWC  +GKTY SEQE+++RL+VFEENYAYI
Sbjct: 1    MSWLCPSLVLVLLIFQGPICTCSSISDLFESWCQQNGKTYSSEQERVYRLEVFEENYAYI 60

Query: 259  IHHNNMANSSYILSLNAFADLTPHEFKASRLGLSAAANGLTRMNRGSS---VQESSGSRD 429
            I HN+  NS+Y L+LNAF+DLT HEFK S LGLS++AN   R+  GSS   V    G  D
Sbjct: 61   IEHNSKGNSTYTLNLNAFSDLTHHEFKNSFLGLSSSANDFIRLKTGSSSAGVFNDVGVVD 120

Query: 430  IPSSLDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRSY 609
            IPSSLDWR+KGAVT VK+QGSCGACWSFSATGA+EGIN+IVTGSL SLSEQEL+DCD+SY
Sbjct: 121  IPSSLDWREKGAVTKVKNQGSCGACWSFSATGAIEGINKIVTGSLVSLSEQELIDCDKSY 180

Query: 610  NSGCGGGLMDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPENDE 789
            N GCGGGLMDY ++FV KN GIDTEEDYP+   E +C+KNKL+R VVTIDGY DVP+ DE
Sbjct: 181  NDGCGGGLMDYAFEFVKKNGGIDTEEDYPFNEREGTCNKNKLQRRVVTIDGYTDVPQYDE 240

Query: 790  KQLLQAVAAQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIVK 969
             +LL+AVA QPVSVGICGSER FQ YSKGIFTGPCST LDHAVLIVGYGSENGVDYWI+K
Sbjct: 241  DKLLKAVANQPVSVGICGSERAFQSYSKGIFTGPCSTVLDHAVLIVGYGSENGVDYWIIK 300

Query: 970  NSWGKQWGMDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXXX 1149
            NSWG  WG++GYM+MQRNSGN EGICGIN +ASY                 KCS+     
Sbjct: 301  NSWGTSWGINGYMHMQRNSGNQEGICGINKLASYPTKSSPNPPSPPSPGPSKCSMFTSCG 360

Query: 1150 XXXXXXXXWRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
                    WRLLGVC+SWKCC ++SAVCCKD  HCCPHD
Sbjct: 361  QGETCCCGWRLLGVCVSWKCCGLDSAVCCKDGRHCCPHD 399


>ref|XP_009597081.1| PREDICTED: zingipain-2 [Nicotiana tomentosiformis]
          Length = 439

 Score =  560 bits (1442), Expect = 0.0
 Identities = 268/399 (67%), Positives = 307/399 (76%), Gaps = 3/399 (0%)
 Frame = +1

Query: 79   MSWVCLFLGLTLLRSVPLLCSSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAYI 258
            MSW+C  L L LL     +C+ S  SDLF+SWC  +GKTY SEQE+++RL+VFEENYAYI
Sbjct: 1    MSWLCPSLVLVLLIFQGPICTCSSISDLFESWCQQNGKTYSSEQERVYRLEVFEENYAYI 60

Query: 259  IHHNNMANSSYILSLNAFADLTPHEFKASRLGLSAAANGLTRMNRGSS---VQESSGSRD 429
            I HN+  NS+Y L LNAF+DLT HEFK S LGLS++AN   R+  GSS   V    G  D
Sbjct: 61   IEHNSKGNSTYTLDLNAFSDLTHHEFKNSFLGLSSSANDFIRLKTGSSSAGVFNDVGVVD 120

Query: 430  IPSSLDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRSY 609
            IPSSLDWR+KGAVT VK+QGSCGACWSFSATGA+EGIN+IVTGSL SLSEQEL+DCD+SY
Sbjct: 121  IPSSLDWREKGAVTKVKNQGSCGACWSFSATGAIEGINKIVTGSLVSLSEQELIDCDKSY 180

Query: 610  NSGCGGGLMDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPENDE 789
            N GCGGGLMDY ++FV KN GIDTEEDYP+   E +C+KNKL+R VVTIDGY DVP+ DE
Sbjct: 181  NDGCGGGLMDYAFEFVKKNGGIDTEEDYPFNEREGTCNKNKLQRRVVTIDGYTDVPQYDE 240

Query: 790  KQLLQAVAAQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIVK 969
             +LL+AVA QPVSVGICGSER FQ YSKGIFTGPCST LDHAVLIVGYGSENGVDYWI+K
Sbjct: 241  DKLLKAVANQPVSVGICGSERAFQSYSKGIFTGPCSTVLDHAVLIVGYGSENGVDYWIIK 300

Query: 970  NSWGKQWGMDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXXX 1149
            NSWG  WG++GYM+MQRNSGN EGICGIN +ASY                 KCS+     
Sbjct: 301  NSWGTSWGINGYMHMQRNSGNQEGICGINKLASYPTKSSPNPPSPPSPGPSKCSMFTSCG 360

Query: 1150 XXXXXXXXWRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
                    WRLLGVC+SWKCC ++SAVCCKD  HCCPHD
Sbjct: 361  QGETCCCGWRLLGVCVSWKCCGLDSAVCCKDGRHCCPHD 399


>ref|XP_019244955.1| PREDICTED: zingipain-2 [Nicotiana attenuata]
 gb|OIT04004.1| putative cysteine protease rd21c [Nicotiana attenuata]
          Length = 439

 Score =  555 bits (1429), Expect = 0.0
 Identities = 265/399 (66%), Positives = 306/399 (76%), Gaps = 3/399 (0%)
 Frame = +1

Query: 79   MSWVCLFLGLTLLRSVPLLCSSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAYI 258
            MSW+  FL L L+     +C+ S  SDLF+SWC  +GK+Y SEQE+++RLKVFEENYAYI
Sbjct: 1    MSWLWSFLVLVLVIFQGPICTCSSISDLFESWCQQNGKSYSSEQERVYRLKVFEENYAYI 60

Query: 259  IHHNNMANSSYILSLNAFADLTPHEFKASRLGLSAAANGLTRMNRGSS---VQESSGSRD 429
            I HN+  N +Y L LNA++DLT HEFK   LGLS++AN   R+  GSS   V    G  D
Sbjct: 61   IEHNSKGNYTYTLGLNAYSDLTHHEFKNYFLGLSSSANDFIRLKTGSSSAGVASDVGVVD 120

Query: 430  IPSSLDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRSY 609
            IPSSLDWR+KGAVT VK+QGSCGACWSFSATGA+EGIN+IVTGSL SLSEQEL+DCD+SY
Sbjct: 121  IPSSLDWREKGAVTKVKNQGSCGACWSFSATGAIEGINKIVTGSLVSLSEQELIDCDKSY 180

Query: 610  NSGCGGGLMDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPENDE 789
            N GCGGGLMDY ++FV KN GIDTEEDYP+   E +C+KNKL+R VVTIDGY DVP+NDE
Sbjct: 181  NDGCGGGLMDYAFEFVKKNGGIDTEEDYPFIEREGTCNKNKLQRRVVTIDGYTDVPQNDE 240

Query: 790  KQLLQAVAAQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIVK 969
             +LL+AVA QPVSVGICGSER FQ YSKGIFTGPCST LDHAVLIVGYGSENGVDYWI+K
Sbjct: 241  DKLLKAVAKQPVSVGICGSERAFQSYSKGIFTGPCSTALDHAVLIVGYGSENGVDYWIIK 300

Query: 970  NSWGKQWGMDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXXX 1149
            NSWG  WG++GYM+MQRNSGN EGICGIN +ASY                 KCS+     
Sbjct: 301  NSWGTSWGINGYMHMQRNSGNQEGICGINKLASYPTKSSPNPPSPPSPGPSKCSMFTSCG 360

Query: 1150 XXXXXXXXWRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
                    W+LLGVCLSWKCC ++SAVCCKD  HCCPHD
Sbjct: 361  QGETCCCGWKLLGVCLSWKCCGLDSAVCCKDGRHCCPHD 399


>ref|XP_010271530.1| PREDICTED: low-temperature-induced cysteine proteinase [Nelumbo
            nucifera]
          Length = 443

 Score =  553 bits (1426), Expect = 0.0
 Identities = 268/398 (67%), Positives = 315/398 (79%), Gaps = 2/398 (0%)
 Frame = +1

Query: 79   MSWVCLFLGLTLLRSVPLLCSSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAYI 258
            +S   L L L +   V L  SS  TSDLFD WC  HG+TY SE+E+LFRLKVFE+N A++
Sbjct: 3    LSLSSLLLLLVIFSHVYLSYSS--TSDLFDRWCEEHGRTYSSEEERLFRLKVFEDNLAFV 60

Query: 259  IHHNNMANSSYILSLNAFADLTPHEFKASRLGLSAAANGLTRMN-RGSSVQES-SGSRDI 432
              HN+MANS+Y L+LNAFADLT HEFK SRLGL+AAA  + R + R  S+ ES S +  +
Sbjct: 61   TEHNSMANSTYSLALNAFADLTHHEFKISRLGLAAAATDMVRSSPRAPSLIESPSIAGQL 120

Query: 433  PSSLDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRSYN 612
            PSS+DWR+KGAVTNVKDQGSCGACWSFSATGA+EGIN+IVTGS  SLSEQELVDCDRSYN
Sbjct: 121  PSSIDWREKGAVTNVKDQGSCGACWSFSATGAIEGINKIVTGSPLSLSEQELVDCDRSYN 180

Query: 613  SGCGGGLMDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPENDEK 792
            SGCGGGLMDY +Q+V+KNKGIDTE+DYPYQ  E++C+K+KL++HVVTIDGY DVP N EK
Sbjct: 181  SGCGGGLMDYAFQWVIKNKGIDTEDDYPYQGGERTCNKDKLRKHVVTIDGYTDVPSNSEK 240

Query: 793  QLLQAVAAQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIVKN 972
             LLQAVA+QPVSVGICGSER FQLYSKGIF+GPCST LDHAVLIVGYGSENGVDYWIVKN
Sbjct: 241  HLLQAVASQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKN 300

Query: 973  SWGKQWGMDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXXXX 1152
            SWG +WGM+GYM+M RNSG+ +G+CGINM+ASY                 +C LL     
Sbjct: 301  SWGTRWGMNGYMHMLRNSGSPQGVCGINMLASYPTKTSPNPPPSPSPGPTRCDLLTYCQE 360

Query: 1153 XXXXXXXWRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
                    R+LG+C SWKCCE++SAVCCKD+ +CCPHD
Sbjct: 361  GETCCCTRRILGICFSWKCCELDSAVCCKDHRYCCPHD 398


>ref|XP_009771293.1| PREDICTED: zingipain-2 [Nicotiana sylvestris]
 ref|XP_016497810.1| PREDICTED: zingipain-2-like [Nicotiana tabacum]
          Length = 439

 Score =  552 bits (1422), Expect = 0.0
 Identities = 263/399 (65%), Positives = 306/399 (76%), Gaps = 3/399 (0%)
 Frame = +1

Query: 79   MSWVCLFLGLTLLRSVPLLCSSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAYI 258
            MSW+   L + LL     +C+ S  SDLF++WC  +GK+Y SEQE+++RLKVFEENYAYI
Sbjct: 1    MSWLWPSLVIVLLIFQGPICTCSSISDLFETWCQQNGKSYSSEQERVYRLKVFEENYAYI 60

Query: 259  IHHNNMANSSYILSLNAFADLTPHEFKASRLGLSAAANGLTRMNRGSS---VQESSGSRD 429
            I HN+  NS+Y L LNA++DLT HEFK S LGLS++AN   R+  GSS   V    G  D
Sbjct: 61   IEHNSKGNSTYTLGLNAYSDLTHHEFKNSFLGLSSSANDFIRLKTGSSSAGVFSDVGDVD 120

Query: 430  IPSSLDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRSY 609
            +PSSLDWR+KGAVT VK+QGSCGACWSFSATGA+EGIN+IV+GSL SLSEQEL+DCD+SY
Sbjct: 121  VPSSLDWREKGAVTKVKNQGSCGACWSFSATGAIEGINKIVSGSLVSLSEQELIDCDKSY 180

Query: 610  NSGCGGGLMDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPENDE 789
            N GCGGGLMDY ++FV KN GIDTEEDYP+   E +C+KNKL+R VVTIDGY DVP+NDE
Sbjct: 181  NDGCGGGLMDYAFEFVKKNGGIDTEEDYPFIEREGTCNKNKLQRRVVTIDGYTDVPQNDE 240

Query: 790  KQLLQAVAAQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIVK 969
             +LL+AVA QPVSVGICGSER FQ YSKGIFTGPCST LDHAVLIVGYGSENGVDYWI+K
Sbjct: 241  DKLLKAVAKQPVSVGICGSERAFQSYSKGIFTGPCSTALDHAVLIVGYGSENGVDYWIIK 300

Query: 970  NSWGKQWGMDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXXX 1149
            NSWG  WG++GYM+MQRNSGN EGICGIN +ASY                 KCS+     
Sbjct: 301  NSWGTSWGINGYMHMQRNSGNQEGICGINKLASYPTKSSPNPPSPPSPGPSKCSMFTSCG 360

Query: 1150 XXXXXXXXWRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
                    W LLGVCLSWKCC ++SAVCCKD  HCCPHD
Sbjct: 361  QGETCCCGWSLLGVCLSWKCCGLDSAVCCKDGRHCCPHD 399


>ref|XP_004152671.1| PREDICTED: zingipain-2 [Cucumis sativus]
 gb|KGN62639.1| hypothetical protein Csa_2G362510 [Cucumis sativus]
          Length = 431

 Score =  549 bits (1414), Expect = 0.0
 Identities = 266/390 (68%), Positives = 298/390 (76%)
 Frame = +1

Query: 97   FLGLTLLRSVPLLCSSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAYIIHHNNM 276
            FL L LL   PL  +S+  S+LF+ WC  HGK+Y S +EKL+RL VF +NY ++ HHNN+
Sbjct: 8    FLTLFLLLFRPLSATSN-VSELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFVTHHNNL 66

Query: 277  ANSSYILSLNAFADLTPHEFKASRLGLSAAANGLTRMNRGSSVQESSGSRDIPSSLDWRK 456
             NSSY LSLN++ADLT HEFK SRLG S A        R    QE S  RD+P SLDWRK
Sbjct: 67   DNSSYTLSLNSYADLTHHEFKVSRLGFSPALRNF----RPVLPQEPSLPRDVPDSLDWRK 122

Query: 457  KGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRSYNSGCGGGLM 636
            KGAVT VKDQGSCGACWSFSATGAMEGINQI+TGSL SLSEQEL+DCDRSYNSGCGGGLM
Sbjct: 123  KGAVTAVKDQGSCGACWSFSATGAMEGINQIMTGSLISLSEQELIDCDRSYNSGCGGGLM 182

Query: 637  DYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPENDEKQLLQAVAA 816
            DY YQFV+ N GIDTE DYPYQ  + SC K+KL+R+VVTIDGY D+P NDE +LLQAVAA
Sbjct: 183  DYAYQFVISNHGIDTENDYPYQARDGSCRKDKLQRNVVTIDGYADIPSNDEGKLLQAVAA 242

Query: 817  QPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIVKNSWGKQWGM 996
            QPVSVGICGSER FQLYSKGIF+GPCST LDHAVLIVGYGSENGVDYWIVKNSWGK WGM
Sbjct: 243  QPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGM 302

Query: 997  DGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXXXXXXXXXXXW 1176
            DGYM+MQRNSGNSEG+CGIN +ASY                 KCS+L             
Sbjct: 303  DGYMHMQRNSGNSEGVCGINKLASYPTKTNPNPPPSPPPGPTKCSILTSCAAGETCCCAK 362

Query: 1177 RLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
            + LG+CLSWKCC ++SAVCCKD  HCCP D
Sbjct: 363  KFLGLCLSWKCCGLSSAVCCKDGRHCCPFD 392


>ref|XP_022769083.1| low-temperature-induced cysteine proteinase [Durio zibethinus]
 ref|XP_022769084.1| low-temperature-induced cysteine proteinase [Durio zibethinus]
          Length = 440

 Score =  549 bits (1414), Expect = 0.0
 Identities = 264/389 (67%), Positives = 297/389 (76%)
 Frame = +1

Query: 100  LGLTLLRSVPLLCSSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAYIIHHNNMA 279
            L   L   +    S S  S LF++WC  HGK Y SE+EK +R+KVFE+NY ++  HN+MA
Sbjct: 10   LSFLLFFDLSFASSPSHISHLFETWCDEHGKKYSSEEEKSYRIKVFEDNYGFVTQHNDMA 69

Query: 280  NSSYILSLNAFADLTPHEFKASRLGLSAAANGLTRMNRGSSVQESSGSRDIPSSLDWRKK 459
            NSSY L+LNAFADLT HEFKASRLGL AAA      +R  ++QE    + IP SLDWRKK
Sbjct: 70   NSSYTLALNAFADLTHHEFKASRLGLFAAA--AIDHHRQPNLQEPGLLQGIPESLDWRKK 127

Query: 460  GAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRSYNSGCGGGLMD 639
            GAVT VKDQGSCGACWSFSATGA+EGIN+IV GSL SLSEQELVDCDRSYNSGC GGLMD
Sbjct: 128  GAVTQVKDQGSCGACWSFSATGAIEGINKIVIGSLVSLSEQELVDCDRSYNSGCEGGLMD 187

Query: 640  YVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPENDEKQLLQAVAAQ 819
            Y YQFV+ N GIDTEEDYPYQT EK+C+K KLKR VVTIDGY D+P N+EKQLLQAVA Q
Sbjct: 188  YAYQFVIDNHGIDTEEDYPYQTQEKTCNKEKLKRRVVTIDGYTDIPANNEKQLLQAVATQ 247

Query: 820  PVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIVKNSWGKQWGMD 999
            PVSVGICGSER FQLYSKGIFTGPCS  LDHAVLIVGYGSENG+DYWIVKNSWG +WGM+
Sbjct: 248  PVSVGICGSERAFQLYSKGIFTGPCSNSLDHAVLIVGYGSENGMDYWIVKNSWGTRWGMN 307

Query: 1000 GYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXXXXXXXXXXXWR 1179
            GY++M RN GNSEGICGINM+ASY                 KC+L              R
Sbjct: 308  GYIHMIRNGGNSEGICGINMLASYPTKTSPNPPPPPPPGPTKCNLFTYCSAGETCCCTHR 367

Query: 1180 LLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
            + G+C SWKCCE++SAVCCKDN HCCPHD
Sbjct: 368  VFGICFSWKCCELDSAVCCKDNRHCCPHD 396


>ref|XP_008444761.1| PREDICTED: zingipain-2 [Cucumis melo]
          Length = 431

 Score =  548 bits (1411), Expect = 0.0
 Identities = 264/390 (67%), Positives = 299/390 (76%)
 Frame = +1

Query: 97   FLGLTLLRSVPLLCSSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAYIIHHNNM 276
            FL L LL   PL  +S+  S+LF+ WC  HGK+Y S +EKL+RL VF +NY ++ HHNN+
Sbjct: 8    FLTLFLLFFRPLFATSN-VSELFEIWCTEHGKSYSSAEEKLYRLSVFADNYEFVTHHNNL 66

Query: 277  ANSSYILSLNAFADLTPHEFKASRLGLSAAANGLTRMNRGSSVQESSGSRDIPSSLDWRK 456
             NSSY LSLN++ADLT HEFK SRLG S A        R    QE S  RD+P SLDWRK
Sbjct: 67   GNSSYTLSLNSYADLTHHEFKVSRLGFSPALRNF----RPVLPQEPSLPRDVPDSLDWRK 122

Query: 457  KGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRSYNSGCGGGLM 636
            KGAVT VKDQGSCGACWSFSATGA+EGINQI+TGSL S+SEQEL+DCDRSYNSGCGGGLM
Sbjct: 123  KGAVTAVKDQGSCGACWSFSATGAIEGINQIMTGSLISVSEQELIDCDRSYNSGCGGGLM 182

Query: 637  DYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPENDEKQLLQAVAA 816
            DY YQFV+ N GIDTE+DYPYQ  + SC K+KL+R+VVTIDGY D+P NDE +LLQAVAA
Sbjct: 183  DYAYQFVISNHGIDTEDDYPYQGRDGSCRKDKLQRNVVTIDGYTDIPPNDEGKLLQAVAA 242

Query: 817  QPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIVKNSWGKQWGM 996
            QPVSVGICGSER FQLYSKGIF+GPCST LDHAVLIVGYGSENGVDYWIVKNSWGK WGM
Sbjct: 243  QPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGM 302

Query: 997  DGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXXXXXXXXXXXW 1176
            DGYM+MQRNSGNSEG+CGIN +ASY                 KCS+L             
Sbjct: 303  DGYMHMQRNSGNSEGVCGINKLASYPTKTSPNPPPSPPPGPTKCSILTSCAAGETCCCAK 362

Query: 1177 RLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
            + LG+CLSWKCC ++SAVCCKD  HCCP D
Sbjct: 363  KFLGLCLSWKCCGLSSAVCCKDGRHCCPFD 392


>ref|XP_012071947.1| low-temperature-induced cysteine proteinase [Jatropha curcas]
 dbj|BAJ53169.1| JHL18I08.3 [Jatropha curcas]
 gb|KDP38570.1| hypothetical protein JCGZ_04495 [Jatropha curcas]
          Length = 441

 Score =  546 bits (1406), Expect = 0.0
 Identities = 258/391 (65%), Positives = 302/391 (77%)
 Frame = +1

Query: 94   LFLGLTLLRSVPLLCSSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAYIIHHNN 273
            LF+   L        SSS  + LF++WC  HGKTY S++EKLFRLKVF++NY ++  HN+
Sbjct: 7    LFVAFLLSYLFLFSSSSSEIAHLFETWCQQHGKTYASQEEKLFRLKVFQDNYDFVTEHNS 66

Query: 274  MANSSYILSLNAFADLTPHEFKASRLGLSAAANGLTRMNRGSSVQESSGSRDIPSSLDWR 453
              NSSY LSLNAFADLT HEFKASRLGLS+AA+    ++R S+ Q      D+P+S+DWR
Sbjct: 67   QGNSSYTLSLNAFADLTHHEFKASRLGLSSAASASLNVDR-SNRQIPDFVADVPASVDWR 125

Query: 454  KKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRSYNSGCGGGL 633
            K GAVT VKDQG+CGACWSFSATGA+EGIN+IVTGSL SLSEQELVDCD+SYN+GC GG+
Sbjct: 126  KNGAVTQVKDQGNCGACWSFSATGAIEGINKIVTGSLVSLSEQELVDCDKSYNNGCEGGI 185

Query: 634  MDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPENDEKQLLQAVA 813
            MDY +QFV+ N GIDTEEDYPYQ  ++SC+K KLKRHVVTIDGY+DVP+N+EK+LL+AVA
Sbjct: 186  MDYAFQFVIDNHGIDTEEDYPYQGRDRSCNKEKLKRHVVTIDGYVDVPQNNEKELLKAVA 245

Query: 814  AQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIVKNSWGKQWG 993
             QPVSVGICGSER FQLYSKGIFTGPCST LDHAVLIVGYGSENGVDYWIVKNSWG  WG
Sbjct: 246  NQPVSVGICGSERAFQLYSKGIFTGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGSYWG 305

Query: 994  MDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXXXXXXXXXXX 1173
            MDGYM+MQRNSG+S G+CGINM+ASY                 +C L             
Sbjct: 306  MDGYMHMQRNSGSSRGLCGINMLASYPKKTSPNPPPPAPPGPTRCDLFTHCGEGETCCCV 365

Query: 1174 WRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
              + G+CLSWKCCE++SAVCCKD  HCCP D
Sbjct: 366  HHIFGICLSWKCCELDSAVCCKDGRHCCPRD 396


>gb|EEF52646.1| cysteine protease, putative [Ricinus communis]
          Length = 422

 Score =  545 bits (1403), Expect = 0.0
 Identities = 264/394 (67%), Positives = 303/394 (76%), Gaps = 3/394 (0%)
 Frame = +1

Query: 94   LFLGLTLL---RSVPLLCSSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAYIIH 264
            LFL +TLL    S+    SSS  S LF+SW   HGKTY S+++KL+R K+FEENY ++  
Sbjct: 7    LFL-ITLLFFNLSISSFSSSSDISKLFESWTKEHGKTYTSKEDKLYRFKIFEENYEFVKK 65

Query: 265  HNNMANSSYILSLNAFADLTPHEFKASRLGLSAAANGLTRMNRGSSVQESSGSRDIPSSL 444
            HN+  NSSY LSLNAFADLT HEFKASRLGLSA +       R   + +  G  D+P S+
Sbjct: 66   HNSQGNSSYTLSLNAFADLTHHEFKASRLGLSAFSTSGKLSRRNFPLHDFVG--DVPISI 123

Query: 445  DWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRSYNSGCG 624
            DWRKKGAV+ VKDQG+CGACWSFSATGA+EGIN+IVTGSL SLSEQELVDCDRSYN+GC 
Sbjct: 124  DWRKKGAVSQVKDQGNCGACWSFSATGAIEGINKIVTGSLVSLSEQELVDCDRSYNNGCE 183

Query: 625  GGLMDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPENDEKQLLQ 804
            GGLMDY YQFV++N GIDTEEDYPYQ  EK+C+K KLKRHVVTIDGY DVP+N+EK+LL+
Sbjct: 184  GGLMDYAYQFVIENNGIDTEEDYPYQAREKTCNKEKLKRHVVTIDGYTDVPQNNEKELLK 243

Query: 805  AVAAQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIVKNSWGK 984
            AVAAQPVSVGICGSER FQLYSKGIFTGPCST LDHAVLIVGYGSENGVDYWIVKNSWG 
Sbjct: 244  AVAAQPVSVGICGSERAFQLYSKGIFTGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGT 303

Query: 985  QWGMDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXXXXXXXX 1164
             WG++GYMYM RNSGNS+G+CGINM+AS+                 KC L          
Sbjct: 304  HWGINGYMYMLRNSGNSQGLCGINMLASFPVKTSPNPPPPAPPGPTKCDLFTRCGEGETC 363

Query: 1165 XXXWRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
                R+ G+C SWKCCE++SAVCCKD  HCCPHD
Sbjct: 364  CCTRRIFGLCFSWKCCELDSAVCCKDGLHCCPHD 397


>ref|XP_023759345.1| low-temperature-induced cysteine proteinase isoform X2 [Lactuca
            sativa]
          Length = 411

 Score =  543 bits (1398), Expect = 0.0
 Identities = 261/400 (65%), Positives = 304/400 (76%), Gaps = 4/400 (1%)
 Frame = +1

Query: 79   MSWVCLFLGLTLLRSVPLLC-SSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAY 255
            M W+     L      PL   S+S+TSDLF  WC  HGK+Y +E+E L+R  VFE+NYAY
Sbjct: 1    MGWLWWISALFFFFLFPLTSLSTSYTSDLFRQWCQQHGKSYSTEEENLYRFNVFEDNYAY 60

Query: 256  IIHHNNMANSSYILSLNAFADLTPHEFKASRL---GLSAAANGLTRMNRGSSVQESSGSR 426
            II HN+ +NSSY LSLN+FADLT HEFK +RL     SAA++ L R+NRGSS+ ESS   
Sbjct: 61   IIRHNSNSNSSYTLSLNSFADLTHHEFKLARLRGLSASAASDDLIRLNRGSSLIESSSY- 119

Query: 427  DIPSSLDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRS 606
             +P SLDWR KGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSL SLSEQELVDCD+S
Sbjct: 120  -LPKSLDWRDKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLISLSEQELVDCDKS 178

Query: 607  YNSGCGGGLMDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPEND 786
            +NSGC GGLMDY Y+FV+KNKGIDTE+DYPYQ  + SC+KNK KR++VTIDGY D+PEN+
Sbjct: 179  FNSGCEGGLMDYAYEFVIKNKGIDTEDDYPYQAKQTSCNKNKRKRNIVTIDGYNDIPENN 238

Query: 787  EKQLLQAVAAQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIV 966
            E QLL+AVA QPVSVGICGSER FQLYSKGIFTGPCST LDHAVLIVGY S++GVDYWI+
Sbjct: 239  EDQLLKAVANQPVSVGICGSERAFQLYSKGIFTGPCSTALDHAVLIVGYDSKDGVDYWII 298

Query: 967  KNSWGKQWGMDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXX 1146
            KNSWG  WG++GYMYM RN+G+S G+CGINM+ASY                 KC+L    
Sbjct: 299  KNSWGNSWGINGYMYMARNTGDSHGLCGINMLASYPIKTSPNPPPSPTPKPVKCNLFSWC 358

Query: 1147 XXXXXXXXXWRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
                        LG+CL W CCE+N+AVCCKD+ HCCP D
Sbjct: 359  SEGETCCCASNFLGICLKWTCCELNAAVCCKDHRHCCPSD 398


>ref|XP_022862440.1| zingipain-2 [Olea europaea var. sylvestris]
          Length = 431

 Score =  543 bits (1400), Expect = 0.0
 Identities = 262/400 (65%), Positives = 299/400 (74%), Gaps = 4/400 (1%)
 Frame = +1

Query: 79   MSWVCLFLGLTLLRSVPLLCSSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAYI 258
            M W+  F  L LL      C SS  SDLF+SWC  +GKTY S QEK +RLKVFEENYAY+
Sbjct: 1    MHWLWPFGYLVLLFFYVPTCKSSSISDLFNSWCQQYGKTYNSVQEKEYRLKVFEENYAYV 60

Query: 259  IHHNNMANSSYILSLNAFADLTPHEFKASRLGLSAAANGLT-RMNRGSSVQESSG---SR 426
             HHN++ N SY LSLNA+ADLT HEFKA  LGLS + + L  R+NRGSS  E        
Sbjct: 61   SHHNSLGNFSYSLSLNAYADLTQHEFKAKYLGLSPSPDDLIIRLNRGSSPVEGLNLVRES 120

Query: 427  DIPSSLDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRS 606
            DIPSSLDWRKKGAVT VKDQGSCGACWSFSATGA+EGIN+I TGSL SLSEQEL+DCD+S
Sbjct: 121  DIPSSLDWRKKGAVTGVKDQGSCGACWSFSATGALEGINKIKTGSLISLSEQELIDCDKS 180

Query: 607  YNSGCGGGLMDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPEND 786
            YN GCGGGLMDY Y+FV+KN GIDTE+DYPYQ  EK+C+K KLKR VVTID Y+D+P+ D
Sbjct: 181  YNDGCGGGLMDYAYEFVIKNHGIDTEKDYPYQGREKTCNKKKLKRRVVTIDSYVDIPDKD 240

Query: 787  EKQLLQAVAAQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIV 966
            EK+LLQAVA QP+SVGICGS+R FQLYS GIFTGPCST LDHAVLIVGY S++G DYWIV
Sbjct: 241  EKRLLQAVATQPISVGICGSDRSFQLYSTGIFTGPCSTSLDHAVLIVGYDSKDGKDYWIV 300

Query: 967  KNSWGKQWGMDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXX 1146
            KNSWGK WGMDGYM+M RN+GN EG+CG+N +ASY                 +C+L    
Sbjct: 301  KNSWGKSWGMDGYMHMLRNNGNPEGVCGVNTLASYPVKTSPNPPPSPSPTPTRCNLFTYC 360

Query: 1147 XXXXXXXXXWRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
                      R+LG+CL WKCC   SAVCCKD  HCCPHD
Sbjct: 361  SSGETCCCARRILGLCLKWKCCGAESAVCCKDRIHCCPHD 400


>ref|XP_002510459.2| PREDICTED: low-temperature-induced cysteine proteinase [Ricinus
            communis]
          Length = 466

 Score =  545 bits (1403), Expect = 0.0
 Identities = 264/394 (67%), Positives = 303/394 (76%), Gaps = 3/394 (0%)
 Frame = +1

Query: 94   LFLGLTLL---RSVPLLCSSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAYIIH 264
            LFL +TLL    S+    SSS  S LF+SW   HGKTY S+++KL+R K+FEENY ++  
Sbjct: 7    LFL-ITLLFFNLSISSFSSSSDISKLFESWTKEHGKTYTSKEDKLYRFKIFEENYEFVKK 65

Query: 265  HNNMANSSYILSLNAFADLTPHEFKASRLGLSAAANGLTRMNRGSSVQESSGSRDIPSSL 444
            HN+  NSSY LSLNAFADLT HEFKASRLGLSA +       R   + +  G  D+P S+
Sbjct: 66   HNSQGNSSYTLSLNAFADLTHHEFKASRLGLSAFSTSGKLSRRNFPLHDFVG--DVPISI 123

Query: 445  DWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRSYNSGCG 624
            DWRKKGAV+ VKDQG+CGACWSFSATGA+EGIN+IVTGSL SLSEQELVDCDRSYN+GC 
Sbjct: 124  DWRKKGAVSQVKDQGNCGACWSFSATGAIEGINKIVTGSLVSLSEQELVDCDRSYNNGCE 183

Query: 625  GGLMDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPENDEKQLLQ 804
            GGLMDY YQFV++N GIDTEEDYPYQ  EK+C+K KLKRHVVTIDGY DVP+N+EK+LL+
Sbjct: 184  GGLMDYAYQFVIENNGIDTEEDYPYQAREKTCNKEKLKRHVVTIDGYTDVPQNNEKELLK 243

Query: 805  AVAAQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIVKNSWGK 984
            AVAAQPVSVGICGSER FQLYSKGIFTGPCST LDHAVLIVGYGSENGVDYWIVKNSWG 
Sbjct: 244  AVAAQPVSVGICGSERAFQLYSKGIFTGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGT 303

Query: 985  QWGMDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXXXXXXXX 1164
             WG++GYMYM RNSGNS+G+CGINM+AS+                 KC L          
Sbjct: 304  HWGINGYMYMLRNSGNSQGLCGINMLASFPVKTSPNPPPPAPPGPTKCDLFTRCGEGETC 363

Query: 1165 XXXWRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
                R+ G+C SWKCCE++SAVCCKD  HCCPHD
Sbjct: 364  CCTRRIFGLCFSWKCCELDSAVCCKDGLHCCPHD 397


>gb|PON90985.1| Cyseine protease [Trema orientalis]
          Length = 447

 Score =  544 bits (1401), Expect = 0.0
 Identities = 265/400 (66%), Positives = 304/400 (76%), Gaps = 5/400 (1%)
 Frame = +1

Query: 82   SWVCLFLGLTLLRSVPLLCSSSF-----TSDLFDSWCLHHGKTYYSEQEKLFRLKVFEEN 246
            S+  L L L LL   P LC SS      TS LF++WC  HGK+Y SE+EKL+RL VFE+N
Sbjct: 6    SFSLLSLLLLLLILHPRLCLSSSSPSLSTSQLFEAWCEKHGKSYSSEEEKLYRLGVFEDN 65

Query: 247  YAYIIHHNNMANSSYILSLNAFADLTPHEFKASRLGLSAAANGLTRMNRGSSVQESSGSR 426
             A++  HN M NSSY LSLNAFADLT HEFKASRLG S   +   ++   S   ++   +
Sbjct: 66   MAFVTQHNQMGNSSYTLSLNAFADLTHHEFKASRLGFSPFLSSRPKVGSNS---KTLYVK 122

Query: 427  DIPSSLDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRS 606
            DIP+SLDWRKKGAVT+VKDQGSCGACWSFSATGA+EGIN+IVTGSL SLSEQELVDCD+S
Sbjct: 123  DIPASLDWRKKGAVTSVKDQGSCGACWSFSATGAIEGINKIVTGSLVSLSEQELVDCDKS 182

Query: 607  YNSGCGGGLMDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPEND 786
            +N+GC GGLMDY YQFV+ N GIDTEEDYPYQ  E SC+K KLKRHVVTIDGY DV +++
Sbjct: 183  FNNGCNGGLMDYAYQFVIDNNGIDTEEDYPYQGRETSCNKAKLKRHVVTIDGYTDVAQSN 242

Query: 787  EKQLLQAVAAQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIV 966
            EKQLLQAVA QPVSVGICGSER FQLYSKGIF GPCST LDHAVLIVGY SENGVDYWIV
Sbjct: 243  EKQLLQAVATQPVSVGICGSERAFQLYSKGIFKGPCSTALDHAVLIVGYDSENGVDYWIV 302

Query: 967  KNSWGKQWGMDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXX 1146
            KNSWG QWGM+GY++++RN+GNS+GICGIN++ASY                 KC L    
Sbjct: 303  KNSWGTQWGMNGYIHIERNNGNSQGICGINLLASYPTKTSPNPPTPPPPGPTKCDLFSRC 362

Query: 1147 XXXXXXXXXWRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
                     WR LG+C SWKCC +NSAVCCKD+ HCCPHD
Sbjct: 363  GEGETCCCAWRFLGICFSWKCCGLNSAVCCKDHLHCCPHD 402


>ref|XP_016568491.1| PREDICTED: low-temperature-induced cysteine proteinase [Capsicum
            annuum]
 gb|PHT51250.1| Xylem cysteine proteinase 1 [Capsicum baccatum]
 gb|PHT84869.1| Xylem cysteine proteinase 1 [Capsicum annuum]
          Length = 441

 Score =  543 bits (1400), Expect = 0.0
 Identities = 255/401 (63%), Positives = 300/401 (74%), Gaps = 5/401 (1%)
 Frame = +1

Query: 79   MSWVCLFLGLTLLRSVPLLCSSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAYI 258
            MSW+   L L LL     LC+ +  SDLF++WC  HGK Y SEQE+++R KVFEENYAYI
Sbjct: 1    MSWLLPCLVLVLLIFQEPLCTCTSISDLFETWCQQHGKKYSSEQERVYRFKVFEENYAYI 60

Query: 259  IHHNNMANSSYILSLNAFADLTPHEFKASRLGLSAAANGLTRMNRG-----SSVQESSGS 423
            I HN+  NSSY L LN ++DLT HEF+ + LGLS++AN   R+  G     +      G 
Sbjct: 61   IEHNSKGNSSYTLGLNEYSDLTHHEFRETFLGLSSSANDFIRLKHGGLGSGTEAWSDVGD 120

Query: 424  RDIPSSLDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDR 603
             DIPSSLDWR+KGAVTNVK+QGSCGACWSFSATGAMEGIN+I TGSL SLSEQEL+DCDR
Sbjct: 121  VDIPSSLDWREKGAVTNVKNQGSCGACWSFSATGAMEGINKIATGSLVSLSEQELIDCDR 180

Query: 604  SYNSGCGGGLMDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPEN 783
            SYN GCGGGLMDY YQFV+KN GIDTE DYP++  E +C++NKL+RHVVTIDGY D+P+ 
Sbjct: 181  SYNQGCGGGLMDYAYQFVIKNGGIDTENDYPFREREGTCNRNKLQRHVVTIDGYTDIPQF 240

Query: 784  DEKQLLQAVAAQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWI 963
            DE +LL+AVA QPVSVGICGS R FQ YSKGIFTGPCST LDHAVLIVGYGSENG+DYWI
Sbjct: 241  DEDKLLKAVATQPVSVGICGSARAFQSYSKGIFTGPCSTSLDHAVLIVGYGSENGLDYWI 300

Query: 964  VKNSWGKQWGMDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXX 1143
            +KNSWG  WG++GYM+M RNSG+ +GICGIN +ASY                 KCS+   
Sbjct: 301  IKNSWGTTWGINGYMHMLRNSGDRDGICGINKLASYPTKSSPNPPTPPAPSPSKCSMFTS 360

Query: 1144 XXXXXXXXXXWRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
                      W++LGVC SWKCC ++SAVCCKD  HCCPHD
Sbjct: 361  CGPGETCCCGWKVLGVCFSWKCCALDSAVCCKDGRHCCPHD 401


>ref|XP_023759344.1| low-temperature-induced cysteine proteinase isoform X1 [Lactuca
            sativa]
 gb|PLY88863.1| hypothetical protein LSAT_5X25740 [Lactuca sativa]
          Length = 442

 Score =  543 bits (1398), Expect = 0.0
 Identities = 261/400 (65%), Positives = 304/400 (76%), Gaps = 4/400 (1%)
 Frame = +1

Query: 79   MSWVCLFLGLTLLRSVPLLC-SSSFTSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAY 255
            M W+     L      PL   S+S+TSDLF  WC  HGK+Y +E+E L+R  VFE+NYAY
Sbjct: 1    MGWLWWISALFFFFLFPLTSLSTSYTSDLFRQWCQQHGKSYSTEEENLYRFNVFEDNYAY 60

Query: 256  IIHHNNMANSSYILSLNAFADLTPHEFKASRL---GLSAAANGLTRMNRGSSVQESSGSR 426
            II HN+ +NSSY LSLN+FADLT HEFK +RL     SAA++ L R+NRGSS+ ESS   
Sbjct: 61   IIRHNSNSNSSYTLSLNSFADLTHHEFKLARLRGLSASAASDDLIRLNRGSSLIESSSY- 119

Query: 427  DIPSSLDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRS 606
             +P SLDWR KGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSL SLSEQELVDCD+S
Sbjct: 120  -LPKSLDWRDKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLISLSEQELVDCDKS 178

Query: 607  YNSGCGGGLMDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPEND 786
            +NSGC GGLMDY Y+FV+KNKGIDTE+DYPYQ  + SC+KNK KR++VTIDGY D+PEN+
Sbjct: 179  FNSGCEGGLMDYAYEFVIKNKGIDTEDDYPYQAKQTSCNKNKRKRNIVTIDGYNDIPENN 238

Query: 787  EKQLLQAVAAQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIV 966
            E QLL+AVA QPVSVGICGSER FQLYSKGIFTGPCST LDHAVLIVGY S++GVDYWI+
Sbjct: 239  EDQLLKAVANQPVSVGICGSERAFQLYSKGIFTGPCSTALDHAVLIVGYDSKDGVDYWII 298

Query: 967  KNSWGKQWGMDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXX 1146
            KNSWG  WG++GYMYM RN+G+S G+CGINM+ASY                 KC+L    
Sbjct: 299  KNSWGNSWGINGYMYMARNTGDSHGLCGINMLASYPIKTSPNPPPSPTPKPVKCNLFSWC 358

Query: 1147 XXXXXXXXXWRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
                        LG+CL W CCE+N+AVCCKD+ HCCP D
Sbjct: 359  SEGETCCCASNFLGICLKWTCCELNAAVCCKDHRHCCPSD 398


>ref|XP_024030266.1| ervatamin-B isoform X2 [Morus notabilis]
          Length = 423

 Score =  542 bits (1396), Expect = 0.0
 Identities = 265/398 (66%), Positives = 303/398 (76%), Gaps = 2/398 (0%)
 Frame = +1

Query: 79   MSWVCLFLGLTLLRSVPLLCSSSF-TSDLFDSWCLHHGKTYYSEQEKLFRLKVFEENYAY 255
            M  +CLFL   LL S   L S S  +S LF++WC  HG++Y SE+E+L+RL VFE+N A+
Sbjct: 1    MKILCLFLLSLLLSSHLSLSSPSLNSSQLFEAWCEKHGQSYSSEEERLYRLTVFEDNLAF 60

Query: 256  IIHHNNMANSSYILSLNAFADLTPHEFKASRLGLSAAA-NGLTRMNRGSSVQESSGSRDI 432
            +  HNNM NSSY LSLNAFADLT HEFK+SRLG S+A  + L ++  GS + +    RD+
Sbjct: 61   VTQHNNMGNSSYTLSLNAFADLTHHEFKSSRLGFSSALLSSLPKL--GSKLLDL---RDV 115

Query: 433  PSSLDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGSLTSLSEQELVDCDRSYN 612
            P+SLDWRKKGAVTNVKDQGSCGACW+FSATGA+EGIN+IVTGSL SLSEQEL+DCD SYN
Sbjct: 116  PASLDWRKKGAVTNVKDQGSCGACWAFSATGAIEGINKIVTGSLVSLSEQELIDCDTSYN 175

Query: 613  SGCGGGLMDYVYQFVVKNKGIDTEEDYPYQTAEKSCDKNKLKRHVVTIDGYIDVPENDEK 792
            +GC GGLMDY YQFV+ N GIDTEEDYPYQ  +KSC K KLKR VVTIDGY DV  N+  
Sbjct: 176  AGCDGGLMDYAYQFVIDNHGIDTEEDYPYQARDKSCRKEKLKRRVVTIDGYTDVAPNNGL 235

Query: 793  QLLQAVAAQPVSVGICGSERGFQLYSKGIFTGPCSTYLDHAVLIVGYGSENGVDYWIVKN 972
            QLLQAV  QPVSVGICGSER FQLYSKGIFTGPCST LDHAVLIVGY SENGVDYWIVKN
Sbjct: 236  QLLQAVVTQPVSVGICGSERAFQLYSKGIFTGPCSTSLDHAVLIVGYDSENGVDYWIVKN 295

Query: 973  SWGKQWGMDGYMYMQRNSGNSEGICGINMMASYXXXXXXXXXXXXXXXXXKCSLLXXXXX 1152
            SWGKQWGMDGY++MQRN+GNS+G+CGINM+ASY                 +CS       
Sbjct: 296  SWGKQWGMDGYIHMQRNTGNSQGVCGINMLASYPTKTSPNPPPSPSPGPTRCSFFAQCGE 355

Query: 1153 XXXXXXXWRLLGVCLSWKCCEINSAVCCKDNHHCCPHD 1266
                   WR LG+C SWKCC +NSAVCCKD  HCCP D
Sbjct: 356  GETCCCSWRFLGLCFSWKCCGLNSAVCCKDKIHCCPQD 393