BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Acanthopanax21_contig00021233
         (1328 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_017219394.1| PREDICTED: biotin--protein ligase 2-like iso...   529   0.0  
ref|XP_017219454.1| PREDICTED: biotin--protein ligase 2-like iso...   528   0.0  
ref|XP_021809932.1| biotin--protein ligase 2-like [Prunus avium]      522   0.0  
ref|XP_011027607.1| PREDICTED: biotin--protein ligase 2-like iso...   523   0.0  
ref|XP_017219399.1| PREDICTED: biotin--protein ligase 1, chlorop...   520   0.0  
ref|XP_002301010.2| hypothetical protein POPTR_0002s08830g [Popu...   521   0.0  
ref|XP_007221780.1| biotin--protein ligase 2 [Prunus persica] >g...   516   e-180
ref|XP_011027606.1| PREDICTED: biotin--protein ligase 2-like iso...   517   e-180
ref|XP_015870274.1| PREDICTED: biotin--protein ligase 2-like iso...   516   e-180
ref|XP_017219459.1| PREDICTED: biotin--protein ligase 1, chlorop...   512   e-179
ref|XP_022856813.1| biotin--protein ligase 2-like isoform X1 [Ol...   513   e-179
ref|XP_015902016.1| PREDICTED: biotin--protein ligase 2-like [Zi...   516   e-179
ref|XP_008233907.1| PREDICTED: biotin--protein ligase 2 [Prunus ...   512   e-178
ref|XP_022856814.1| biotin--protein ligase 2-like isoform X2 [Ol...   511   e-178
gb|EOY16190.1| Holocarboxylase synthase 1 isoform 1 [Theobroma c...   509   e-177
ref|XP_023926082.1| biotin--protein ligase 2-like isoform X1 [Qu...   508   e-177
ref|XP_007018965.2| PREDICTED: biotin--protein ligase 2 isoform ...   507   e-176
ref|XP_002307419.1| holocarboxylase synthetase 2 family protein ...   504   e-176
ref|XP_012078166.1| biotin--protein ligase 2 [Jatropha curcas] >...   504   e-175
ref|XP_009366988.1| PREDICTED: biotin--protein ligase 2-like [Py...   502   e-174

>ref|XP_017219394.1| PREDICTED: biotin--protein ligase 2-like isoform X1 [Daucus carota
            subsp. sativus]
 ref|XP_017219395.1| PREDICTED: biotin--protein ligase 2-like isoform X1 [Daucus carota
            subsp. sativus]
 ref|XP_017219396.1| PREDICTED: biotin--protein ligase 2-like isoform X1 [Daucus carota
            subsp. sativus]
 ref|XP_017219397.1| PREDICTED: biotin--protein ligase 2-like isoform X1 [Daucus carota
            subsp. sativus]
          Length = 363

 Score =  529 bits (1363), Expect = 0.0
 Identities = 268/369 (72%), Positives = 309/369 (83%)
 Frame = +2

Query: 35   LSKKFPILSLSKKFPILSLSHPHILPETHKKCLLSLSASAMESQSPCVLVLCGKSSTEKE 214
            +S +F  L  S  F    LS P +   T K    SLS+SAMES+S C+LVL GKS+ +KE
Sbjct: 3    ISPRFFSLKSSPHF----LSLPLLYHNTTKP--FSLSSSAMESESTCILVLAGKSAADKE 56

Query: 215  LAKSMKNNSILKLPENAKVSVFLHLEKEKSFDEESFCINSYMNSLSTARFGRFLIWSPQL 394
            LA  MK ++I KLP+   VS+ LH   E+S +EESF INSYMNSLST  FGRFLIWSP+L
Sbjct: 57   LANYMKASNIPKLPDIRDVSLLLH---EESLNEESFRINSYMNSLSTTCFGRFLIWSPRL 113

Query: 395  PSTHDVVSNNFGDLPIGAVCVADFQYKGRGRSNNVWESPKGCLLFSFTMQMEDGRVVPLV 574
            PSTHDVVS NF +LP+G+VCVAD QYKGRGRS NVWESPKGCL+FSFT+QMEDGR+VPLV
Sbjct: 114  PSTHDVVSKNFEELPVGSVCVADVQYKGRGRSMNVWESPKGCLMFSFTIQMEDGRIVPLV 173

Query: 575  QYVVSLAMTEAIKDVCDRNGVPHLDVRIKWPNDLYLNGLKVGGILCTSTYRSKKFNVSAG 754
            QYVVSLAMTEAI DVC ++GVP+LDVRIKWPNDLYL+GLKVGGILCTSTYRSKKFNVSAG
Sbjct: 174  QYVVSLAMTEAINDVCTKDGVPYLDVRIKWPNDLYLDGLKVGGILCTSTYRSKKFNVSAG 233

Query: 755  VGLNVDNEKPTTSLNSVLKKLTLAADQLQREDLIAAFFNKFESLFNLFMNQGFQPLEELY 934
            +GLNVDNEKPTTSLN++LKKLT  + QLQRED+++AFFNKFE  F+LF  QGF+PLEELY
Sbjct: 234  LGLNVDNEKPTTSLNAILKKLTTVSYQLQREDIVSAFFNKFEIFFDLFATQGFKPLEELY 293

Query: 935  YKTWLHSGQKVTVEEKKEDQDQPVRNEFTIQGLTSSGYLLAIGEDGQMCELHPDGNSFDF 1114
            YKTWLHSGQ+V VEEK +D+D   ++  TIQGLTSSGYLLAIG+D QMCELHPDGNSFDF
Sbjct: 294  YKTWLHSGQRVIVEEKNDDKDHGRQSMVTIQGLTSSGYLLAIGDDNQMCELHPDGNSFDF 353

Query: 1115 FKGLVRRKL 1141
            FKGL+RRKL
Sbjct: 354  FKGLIRRKL 362


>ref|XP_017219454.1| PREDICTED: biotin--protein ligase 2-like isoform X1 [Daucus carota
            subsp. sativus]
 ref|XP_017219455.1| PREDICTED: biotin--protein ligase 2-like isoform X1 [Daucus carota
            subsp. sativus]
 ref|XP_017219456.1| PREDICTED: biotin--protein ligase 2-like isoform X1 [Daucus carota
            subsp. sativus]
 ref|XP_017219457.1| PREDICTED: biotin--protein ligase 2-like isoform X1 [Daucus carota
            subsp. sativus]
          Length = 380

 Score =  528 bits (1359), Expect = 0.0
 Identities = 266/380 (70%), Positives = 310/380 (81%)
 Frame = +2

Query: 2    RFLSLRPTLRFLSKKFPILSLSKKFPILSLSHPHILPETHKKCLLSLSASAMESQSPCVL 181
            RFLSL+P+  FLS   P+LS +   P                   SLS+SAMES+S C+L
Sbjct: 24   RFLSLKPSPHFLS--LPLLSHNPTKPF------------------SLSSSAMESESTCIL 63

Query: 182  VLCGKSSTEKELAKSMKNNSILKLPENAKVSVFLHLEKEKSFDEESFCINSYMNSLSTAR 361
            VL GKS+ +KELA SMK ++ILKLP+   VS+ LH E       ESF INSYMNSLS   
Sbjct: 64   VLAGKSAADKELANSMKVSNILKLPDVPVVSLLLHEESPN----ESFRINSYMNSLSNTC 119

Query: 362  FGRFLIWSPQLPSTHDVVSNNFGDLPIGAVCVADFQYKGRGRSNNVWESPKGCLLFSFTM 541
            FGRFLIWSP+LPSTHDVVS NF +LP+G+VCVAD QYKGRGRS N+WESPKGCL+FSFT+
Sbjct: 120  FGRFLIWSPRLPSTHDVVSKNFEELPVGSVCVADVQYKGRGRSMNIWESPKGCLMFSFTI 179

Query: 542  QMEDGRVVPLVQYVVSLAMTEAIKDVCDRNGVPHLDVRIKWPNDLYLNGLKVGGILCTST 721
            QM+DGR+VPLVQYVVSLAMTEAI DVC ++GVP+LDVRIKWPNDLYL+GLKVGGILCTST
Sbjct: 180  QMQDGRIVPLVQYVVSLAMTEAINDVCTKDGVPYLDVRIKWPNDLYLDGLKVGGILCTST 239

Query: 722  YRSKKFNVSAGVGLNVDNEKPTTSLNSVLKKLTLAADQLQREDLIAAFFNKFESLFNLFM 901
            YRSKKFNVSAG+GLNVDNE+PTTSLN++L+KLT  + QLQRED+++AFFNKFE  FNLF 
Sbjct: 240  YRSKKFNVSAGLGLNVDNERPTTSLNAILRKLTTVSYQLQREDIVSAFFNKFEIFFNLFA 299

Query: 902  NQGFQPLEELYYKTWLHSGQKVTVEEKKEDQDQPVRNEFTIQGLTSSGYLLAIGEDGQMC 1081
             QGF+PLEELYYKTWLHSGQ+V VEEK +++D   ++  TIQGLTSSGYLLAIG+D QMC
Sbjct: 300  TQGFKPLEELYYKTWLHSGQRVIVEEKNDEKDHGRQSMVTIQGLTSSGYLLAIGDDNQMC 359

Query: 1082 ELHPDGNSFDFFKGLVRRKL 1141
            ELHPDGNSFDFFKGL+RRKL
Sbjct: 360  ELHPDGNSFDFFKGLIRRKL 379


>ref|XP_021809932.1| biotin--protein ligase 2-like [Prunus avium]
          Length = 365

 Score =  522 bits (1344), Expect = 0.0
 Identities = 249/343 (72%), Positives = 295/343 (86%)
 Frame = +2

Query: 113  ETHKKCLLSLSASAMESQSPCVLVLCGKSSTEKELAKSMKNNSILKLPENAKVSVFLHLE 292
            +T+    L ++ASAM+  S C LVLCGKSS E E AK++KN+  LKL +N ++S+ LH E
Sbjct: 24   KTNSSLSLRVAASAMDGNSSCALVLCGKSSAENETAKTLKNSGALKLTDNTELSILLHSE 83

Query: 293  KEKSFDEESFCINSYMNSLSTARFGRFLIWSPQLPSTHDVVSNNFGDLPIGAVCVADFQY 472
             EK   E++F ++S+MNSLST RFGRFL+WSP+LPSTHDVVS NF +LPIGAVCVAD QY
Sbjct: 84   SEKPIKEDAFGVDSFMNSLSTNRFGRFLLWSPRLPSTHDVVSQNFCELPIGAVCVADVQY 143

Query: 473  KGRGRSNNVWESPKGCLLFSFTMQMEDGRVVPLVQYVVSLAMTEAIKDVCDRNGVPHLDV 652
            KGRGRS N WESPKGCLLFSFT+QMEDGRVVPL+QYVVSLA+TEAIKDVCD+NG+P++DV
Sbjct: 144  KGRGRSKNAWESPKGCLLFSFTLQMEDGRVVPLIQYVVSLAVTEAIKDVCDKNGLPYVDV 203

Query: 653  RIKWPNDLYLNGLKVGGILCTSTYRSKKFNVSAGVGLNVDNEKPTTSLNSVLKKLTLAAD 832
            +IKWPNDLYLNGLKVGGILCTST++SK+FNVSAG+GLNVDNEKPTT LN+ L++L++   
Sbjct: 204  KIKWPNDLYLNGLKVGGILCTSTFKSKQFNVSAGIGLNVDNEKPTTCLNTFLRELSVTTY 263

Query: 833  QLQREDLIAAFFNKFESLFNLFMNQGFQPLEELYYKTWLHSGQKVTVEEKKEDQDQPVRN 1012
            Q +RED++AAFF+KFE  + LF+NQGFQ LEELYYKTWLHSGQ+V V+EK E  DQ V N
Sbjct: 264  QFRREDILAAFFDKFEKFYELFINQGFQSLEELYYKTWLHSGQRVIVQEKNE--DQVVEN 321

Query: 1013 EFTIQGLTSSGYLLAIGEDGQMCELHPDGNSFDFFKGLVRRKL 1141
              TIQGLTSSGYLLAIG+D QMCELHPDGNSFDFFKGLVRRKL
Sbjct: 322  VVTIQGLTSSGYLLAIGDDNQMCELHPDGNSFDFFKGLVRRKL 364


>ref|XP_011027607.1| PREDICTED: biotin--protein ligase 2-like isoform X2 [Populus
            euphratica]
          Length = 391

 Score =  523 bits (1346), Expect = 0.0
 Identities = 273/385 (70%), Positives = 312/385 (81%), Gaps = 11/385 (2%)
 Frame = +2

Query: 20   PTLRFLSKKFPILSLSKKFPILSLSHPHILPETHKKCLLSLS-----ASAMESQS---PC 175
            P LRFLS   P  SLS     L   H  I P T +K  L+LS     A+AM+S +   PC
Sbjct: 17   PHLRFLS---PRASLS-----LRSLHNFIAP-TSQKSPLTLSRSVAAATAMDSSTSSTPC 67

Query: 176  VLVLCGKSSTEKELAKSMKNNSILKLPENAKVSVFLHLE---KEKSFDEESFCINSYMNS 346
             LVLCGKSS E E+AKS+ NN+ LKLP+N +VS+ LH E   K++  DEESFCI  +MNS
Sbjct: 68   KLVLCGKSSAENEIAKSLMNNNNLKLPDNVQVSILLHSEIINKQQRQDEESFCIERFMNS 127

Query: 347  LSTARFGRFLIWSPQLPSTHDVVSNNFGDLPIGAVCVADFQYKGRGRSNNVWESPKGCLL 526
            LST RFGR LIWSP LPSTHDVVSNNFG+LPIG VCVAD QYKGRGRS NVWESP GCL+
Sbjct: 128  LSTNRFGRLLIWSPILPSTHDVVSNNFGELPIGTVCVADVQYKGRGRSKNVWESPAGCLM 187

Query: 527  FSFTMQMEDGRVVPLVQYVVSLAMTEAIKDVCDRNGVPHLDVRIKWPNDLYLNGLKVGGI 706
            FSFT+QMEDGRVVPL+QYVVSLA+TEAIKDVCD+NG+P +DVRIKWPNDLYLNGLKVGGI
Sbjct: 188  FSFTIQMEDGRVVPLLQYVVSLAVTEAIKDVCDKNGLPRIDVRIKWPNDLYLNGLKVGGI 247

Query: 707  LCTSTYRSKKFNVSAGVGLNVDNEKPTTSLNSVLKKLTLAADQLQREDLIAAFFNKFESL 886
            L TSTY+SKKFNVS G+GLNVDNEKPTT LN+VL++L+ AA  L+RED+++AF NKFE  
Sbjct: 248  LSTSTYKSKKFNVSTGIGLNVDNEKPTTCLNAVLRELSAAACTLRREDIVSAFLNKFEIF 307

Query: 887  FNLFMNQGFQPLEELYYKTWLHSGQKVTVEEKKEDQDQPVRNEFTIQGLTSSGYLLAIGE 1066
            ++LF+N GFQ LEELYYKTWLHSGQ+V ++EK E  +Q V N  TIQGLTSSGYLLAIGE
Sbjct: 308  YDLFINGGFQTLEELYYKTWLHSGQRVIIQEKNE--NQVVENVVTIQGLTSSGYLLAIGE 365

Query: 1067 DGQMCELHPDGNSFDFFKGLVRRKL 1141
            D QMCELHPDGNSFDFFKGLVRRKL
Sbjct: 366  DNQMCELHPDGNSFDFFKGLVRRKL 390


>ref|XP_017219399.1| PREDICTED: biotin--protein ligase 1, chloroplastic-like isoform X2
            [Daucus carota subsp. sativus]
          Length = 327

 Score =  520 bits (1338), Expect = 0.0
 Identities = 253/329 (76%), Positives = 290/329 (88%)
 Frame = +2

Query: 155  MESQSPCVLVLCGKSSTEKELAKSMKNNSILKLPENAKVSVFLHLEKEKSFDEESFCINS 334
            MES+S C+LVL GKS+ +KELA  MK ++I KLP+   VS+ LH   E+S +EESF INS
Sbjct: 1    MESESTCILVLAGKSAADKELANYMKASNIPKLPDIRDVSLLLH---EESLNEESFRINS 57

Query: 335  YMNSLSTARFGRFLIWSPQLPSTHDVVSNNFGDLPIGAVCVADFQYKGRGRSNNVWESPK 514
            YMNSLST  FGRFLIWSP+LPSTHDVVS NF +LP+G+VCVAD QYKGRGRS NVWESPK
Sbjct: 58   YMNSLSTTCFGRFLIWSPRLPSTHDVVSKNFEELPVGSVCVADVQYKGRGRSMNVWESPK 117

Query: 515  GCLLFSFTMQMEDGRVVPLVQYVVSLAMTEAIKDVCDRNGVPHLDVRIKWPNDLYLNGLK 694
            GCL+FSFT+QMEDGR+VPLVQYVVSLAMTEAI DVC ++GVP+LDVRIKWPNDLYL+GLK
Sbjct: 118  GCLMFSFTIQMEDGRIVPLVQYVVSLAMTEAINDVCTKDGVPYLDVRIKWPNDLYLDGLK 177

Query: 695  VGGILCTSTYRSKKFNVSAGVGLNVDNEKPTTSLNSVLKKLTLAADQLQREDLIAAFFNK 874
            VGGILCTSTYRSKKFNVSAG+GLNVDNEKPTTSLN++LKKLT  + QLQRED+++AFFNK
Sbjct: 178  VGGILCTSTYRSKKFNVSAGLGLNVDNEKPTTSLNAILKKLTTVSYQLQREDIVSAFFNK 237

Query: 875  FESLFNLFMNQGFQPLEELYYKTWLHSGQKVTVEEKKEDQDQPVRNEFTIQGLTSSGYLL 1054
            FE  F+LF  QGF+PLEELYYKTWLHSGQ+V VEEK +D+D   ++  TIQGLTSSGYLL
Sbjct: 238  FEIFFDLFATQGFKPLEELYYKTWLHSGQRVIVEEKNDDKDHGRQSMVTIQGLTSSGYLL 297

Query: 1055 AIGEDGQMCELHPDGNSFDFFKGLVRRKL 1141
            AIG+D QMCELHPDGNSFDFFKGL+RRKL
Sbjct: 298  AIGDDNQMCELHPDGNSFDFFKGLIRRKL 326


>ref|XP_002301010.2| hypothetical protein POPTR_0002s08830g [Populus trichocarpa]
 gb|PNT48615.1| hypothetical protein POPTR_002G087700v3 [Populus trichocarpa]
          Length = 390

 Score =  521 bits (1342), Expect = 0.0
 Identities = 272/384 (70%), Positives = 311/384 (80%), Gaps = 10/384 (2%)
 Frame = +2

Query: 20   PTLRFLSKKFPILSLSKKFPILSLSHPHILPETHKKCLLSLS-----ASAMESQS--PCV 178
            P LRFLS   P  SLS     L   H  I P T +K   +LS     A+AM+S S  PC 
Sbjct: 17   PHLRFLS---PQSSLS-----LRSLHNFIAP-TSQKSPFTLSRSVAAATAMDSSSSTPCK 67

Query: 179  LVLCGKSSTEKELAKSMKNNSILKLPENAKVSVFLHLE---KEKSFDEESFCINSYMNSL 349
            LVLCGKSS E E+AKS+ NN+ LKLP+N +VS  LH E   K++  DEESFCI  +MNSL
Sbjct: 68   LVLCGKSSAENEIAKSLMNNNTLKLPDNVQVSTLLHSEIINKQQRQDEESFCIERFMNSL 127

Query: 350  STARFGRFLIWSPQLPSTHDVVSNNFGDLPIGAVCVADFQYKGRGRSNNVWESPKGCLLF 529
            ST +FGR LIWSP LPSTHDVVSNNFG+LPIG VCVAD QYKGRGRS NVWESP GCL+F
Sbjct: 128  STNQFGRLLIWSPILPSTHDVVSNNFGELPIGTVCVADVQYKGRGRSKNVWESPAGCLMF 187

Query: 530  SFTMQMEDGRVVPLVQYVVSLAMTEAIKDVCDRNGVPHLDVRIKWPNDLYLNGLKVGGIL 709
            SFT+QMEDGRVVPL+QYVVSLA+TEAIKDVCD+NG+P +DVRIKWPNDLYLNGLKVGGIL
Sbjct: 188  SFTIQMEDGRVVPLLQYVVSLAVTEAIKDVCDKNGLPRIDVRIKWPNDLYLNGLKVGGIL 247

Query: 710  CTSTYRSKKFNVSAGVGLNVDNEKPTTSLNSVLKKLTLAADQLQREDLIAAFFNKFESLF 889
             TSTY+SKKFNVS G+GLNVDNEKPTT LN+VL++L+ AA  L+RED++AAF NKFE+ +
Sbjct: 248  STSTYKSKKFNVSTGIGLNVDNEKPTTCLNAVLRELSAAACTLRREDIVAAFLNKFENFY 307

Query: 890  NLFMNQGFQPLEELYYKTWLHSGQKVTVEEKKEDQDQPVRNEFTIQGLTSSGYLLAIGED 1069
            +LF+N GF+ LEELYYKTWLHSGQ+V ++EK E  +Q V N  TIQGLTSSGYLLAIGED
Sbjct: 308  DLFINGGFRTLEELYYKTWLHSGQRVIIQEKNE--NQVVENVVTIQGLTSSGYLLAIGED 365

Query: 1070 GQMCELHPDGNSFDFFKGLVRRKL 1141
             QMCELHPDGNSFDFFKGLVRRKL
Sbjct: 366  NQMCELHPDGNSFDFFKGLVRRKL 389


>ref|XP_007221780.1| biotin--protein ligase 2 [Prunus persica]
 gb|ONI34885.1| hypothetical protein PRUPE_1G503900 [Prunus persica]
 gb|ONI34886.1| hypothetical protein PRUPE_1G503900 [Prunus persica]
          Length = 365

 Score =  516 bits (1328), Expect = e-180
 Identities = 245/343 (71%), Positives = 293/343 (85%)
 Frame = +2

Query: 113  ETHKKCLLSLSASAMESQSPCVLVLCGKSSTEKELAKSMKNNSILKLPENAKVSVFLHLE 292
            +T+    L  + SAM+  S C LVLCGKSS E E AK++KN+  LKL +N ++S+ LH E
Sbjct: 24   KTNSSLPLPFAVSAMDGNSSCALVLCGKSSAENETAKTLKNSGALKLTDNTELSILLHSE 83

Query: 293  KEKSFDEESFCINSYMNSLSTARFGRFLIWSPQLPSTHDVVSNNFGDLPIGAVCVADFQY 472
              K   E++F ++S+MNSLST RFGRFL+WSP+LPSTHDV+S NF +LPIGAVCVAD QY
Sbjct: 84   SAKPIKEDAFGVDSFMNSLSTNRFGRFLLWSPRLPSTHDVISQNFCELPIGAVCVADVQY 143

Query: 473  KGRGRSNNVWESPKGCLLFSFTMQMEDGRVVPLVQYVVSLAMTEAIKDVCDRNGVPHLDV 652
            KGRGRS NVWESPKGCLLFSFT+QMEDGRVVPL+QYVVSLA+TEAIKDVCD+NG+P++DV
Sbjct: 144  KGRGRSKNVWESPKGCLLFSFTLQMEDGRVVPLIQYVVSLAVTEAIKDVCDKNGLPYVDV 203

Query: 653  RIKWPNDLYLNGLKVGGILCTSTYRSKKFNVSAGVGLNVDNEKPTTSLNSVLKKLTLAAD 832
            +IKWPNDLYLNGLKVGGILCTST++SK+FNVSAG+GLNVDNEKPTT LN+ L++L++   
Sbjct: 204  KIKWPNDLYLNGLKVGGILCTSTFKSKQFNVSAGIGLNVDNEKPTTCLNTFLRELSVTTY 263

Query: 833  QLQREDLIAAFFNKFESLFNLFMNQGFQPLEELYYKTWLHSGQKVTVEEKKEDQDQPVRN 1012
            Q +RED++AAFF+KFE  + LF+NQGFQ LEELYYKTWLHSGQ+V V+EK +  DQ V N
Sbjct: 264  QFRREDILAAFFDKFEKFYELFINQGFQSLEELYYKTWLHSGQRVIVQEKND--DQVVEN 321

Query: 1013 EFTIQGLTSSGYLLAIGEDGQMCELHPDGNSFDFFKGLVRRKL 1141
              TIQGLTSSGYLLAIG+D QMCELHPDGNSFDFFKGLVR+KL
Sbjct: 322  VVTIQGLTSSGYLLAIGDDNQMCELHPDGNSFDFFKGLVRQKL 364


>ref|XP_011027606.1| PREDICTED: biotin--protein ligase 2-like isoform X1 [Populus
            euphratica]
          Length = 395

 Score =  517 bits (1331), Expect = e-180
 Identities = 273/389 (70%), Positives = 312/389 (80%), Gaps = 15/389 (3%)
 Frame = +2

Query: 20   PTLRFLSKKFPILSLSKKFPILSLSHPHILPETHKKCLLSLS-----ASAMESQS---PC 175
            P LRFLS   P  SLS     L   H  I P T +K  L+LS     A+AM+S +   PC
Sbjct: 17   PHLRFLS---PRASLS-----LRSLHNFIAP-TSQKSPLTLSRSVAAATAMDSSTSSTPC 67

Query: 176  VLVLCGKSSTEKELAKSMKNNSILKLPENAKVSVFLHLE---KEKSFDEESFCINSYMNS 346
             LVLCGKSS E E+AKS+ NN+ LKLP+N +VS+ LH E   K++  DEESFCI  +MNS
Sbjct: 68   KLVLCGKSSAENEIAKSLMNNNNLKLPDNVQVSILLHSEIINKQQRQDEESFCIERFMNS 127

Query: 347  LSTARFGRFLIWSPQLPSTHDVVSNNFGDLPIGAVCVADFQYKGRGRSNNVWESPKGCLL 526
            LST RFGR LIWSP LPSTHDVVSNNFG+LPIG VCVAD QYKGRGRS NVWESP GCL+
Sbjct: 128  LSTNRFGRLLIWSPILPSTHDVVSNNFGELPIGTVCVADVQYKGRGRSKNVWESPAGCLM 187

Query: 527  FSFTMQMEDGRVVPLVQYVVSLAMTEAIKDVCDRNGVPHLDVRIKWPNDLYLNGLKVGGI 706
            FSFT+QMEDGRVVPL+QYVVSLA+TEAIKDVCD+NG+P +DVRIKWPNDLYLNGLKVGGI
Sbjct: 188  FSFTIQMEDGRVVPLLQYVVSLAVTEAIKDVCDKNGLPRIDVRIKWPNDLYLNGLKVGGI 247

Query: 707  LCTSTYRSKKFNVSAGVGLNVDNEKPTTSLNSVLKKLTLAADQLQREDLIAAFFNKFESL 886
            L TSTY+SKKFNVS G+GLNVDNEKPTT LN+VL++L+ AA  L+RED+++AF NKFE  
Sbjct: 248  LSTSTYKSKKFNVSTGIGLNVDNEKPTTCLNAVLRELSAAACTLRREDIVSAFLNKFEIF 307

Query: 887  FNLFMNQGFQPLEELYYKTWLH----SGQKVTVEEKKEDQDQPVRNEFTIQGLTSSGYLL 1054
            ++LF+N GFQ LEELYYKTWLH    SGQ+V ++EK E  +Q V N  TIQGLTSSGYLL
Sbjct: 308  YDLFINGGFQTLEELYYKTWLHRCSCSGQRVIIQEKNE--NQVVENVVTIQGLTSSGYLL 365

Query: 1055 AIGEDGQMCELHPDGNSFDFFKGLVRRKL 1141
            AIGED QMCELHPDGNSFDFFKGLVRRKL
Sbjct: 366  AIGEDNQMCELHPDGNSFDFFKGLVRRKL 394


>ref|XP_015870274.1| PREDICTED: biotin--protein ligase 2-like isoform X2 [Ziziphus jujuba]
          Length = 377

 Score =  516 bits (1329), Expect = e-180
 Identities = 249/336 (74%), Positives = 293/336 (87%), Gaps = 1/336 (0%)
 Frame = +2

Query: 137  SLSASAMESQSPCVLVLCGKSSTEKELAKSMKNNSILKLPENAKVSVFLHLEKEKSFDE- 313
            S S+S+  S  PC LVLCGKSS E E+A+S+KNN+ LKLP+N  VSV L  E +K  D+ 
Sbjct: 43   SSSSSSSSSSPPCKLVLCGKSSAENEIAESLKNNNTLKLPDNDAVSVLLQSEWDKPLDDG 102

Query: 314  ESFCINSYMNSLSTARFGRFLIWSPQLPSTHDVVSNNFGDLPIGAVCVADFQYKGRGRSN 493
            +SF I+S+MNSLST RFGRFL+WSP L STHDVVS+NFG+LP+GAVCVAD QYKGRGRS 
Sbjct: 103  DSFRIDSFMNSLSTNRFGRFLLWSPLLTSTHDVVSHNFGELPVGAVCVADVQYKGRGRSK 162

Query: 494  NVWESPKGCLLFSFTMQMEDGRVVPLVQYVVSLAMTEAIKDVCDRNGVPHLDVRIKWPND 673
            NVW+SPKGCLLFSFT+QMEDGRVVPLVQYVVSLA+TEAIKD+CD+NG+P++DV+IKWPND
Sbjct: 163  NVWQSPKGCLLFSFTLQMEDGRVVPLVQYVVSLAVTEAIKDICDKNGLPYVDVKIKWPND 222

Query: 674  LYLNGLKVGGILCTSTYRSKKFNVSAGVGLNVDNEKPTTSLNSVLKKLTLAADQLQREDL 853
            LYLN LKVGGILCTSTY+SKKFN+SAG+GLNVDNEKPT  LN +L++L++ A Q +RED+
Sbjct: 223  LYLNNLKVGGILCTSTYKSKKFNISAGIGLNVDNEKPTICLNGLLRELSVVAYQFEREDI 282

Query: 854  IAAFFNKFESLFNLFMNQGFQPLEELYYKTWLHSGQKVTVEEKKEDQDQPVRNEFTIQGL 1033
            +A+FFNKFE  ++LFM+QGFQ LEELYYKTWLHSGQ+V V+EK E  DQ V N  T+QGL
Sbjct: 283  LASFFNKFEIFYDLFMDQGFQSLEELYYKTWLHSGQRVIVQEKNE--DQVVENVVTVQGL 340

Query: 1034 TSSGYLLAIGEDGQMCELHPDGNSFDFFKGLVRRKL 1141
            TSSGYLLAIG+D QMCELHPDGNSFDFFKGLVRRKL
Sbjct: 341  TSSGYLLAIGDDNQMCELHPDGNSFDFFKGLVRRKL 376


>ref|XP_017219459.1| PREDICTED: biotin--protein ligase 1, chloroplastic-like isoform X3
            [Daucus carota subsp. sativus]
 ref|XP_017219460.1| PREDICTED: biotin--protein ligase 1, chloroplastic-like isoform X3
            [Daucus carota subsp. sativus]
          Length = 326

 Score =  512 bits (1319), Expect = e-179
 Identities = 248/329 (75%), Positives = 287/329 (87%)
 Frame = +2

Query: 155  MESQSPCVLVLCGKSSTEKELAKSMKNNSILKLPENAKVSVFLHLEKEKSFDEESFCINS 334
            MES+S C+LVL GKS+ +KELA SMK ++ILKLP+   VS+ LH E       ESF INS
Sbjct: 1    MESESTCILVLAGKSAADKELANSMKVSNILKLPDVPVVSLLLHEESPN----ESFRINS 56

Query: 335  YMNSLSTARFGRFLIWSPQLPSTHDVVSNNFGDLPIGAVCVADFQYKGRGRSNNVWESPK 514
            YMNSLS   FGRFLIWSP+LPSTHDVVS NF +LP+G+VCVAD QYKGRGRS N+WESPK
Sbjct: 57   YMNSLSNTCFGRFLIWSPRLPSTHDVVSKNFEELPVGSVCVADVQYKGRGRSMNIWESPK 116

Query: 515  GCLLFSFTMQMEDGRVVPLVQYVVSLAMTEAIKDVCDRNGVPHLDVRIKWPNDLYLNGLK 694
            GCL+FSFT+QM+DGR+VPLVQYVVSLAMTEAI DVC ++GVP+LDVRIKWPNDLYL+GLK
Sbjct: 117  GCLMFSFTIQMQDGRIVPLVQYVVSLAMTEAINDVCTKDGVPYLDVRIKWPNDLYLDGLK 176

Query: 695  VGGILCTSTYRSKKFNVSAGVGLNVDNEKPTTSLNSVLKKLTLAADQLQREDLIAAFFNK 874
            VGGILCTSTYRSKKFNVSAG+GLNVDNE+PTTSLN++L+KLT  + QLQRED+++AFFNK
Sbjct: 177  VGGILCTSTYRSKKFNVSAGLGLNVDNERPTTSLNAILRKLTTVSYQLQREDIVSAFFNK 236

Query: 875  FESLFNLFMNQGFQPLEELYYKTWLHSGQKVTVEEKKEDQDQPVRNEFTIQGLTSSGYLL 1054
            FE  FNLF  QGF+PLEELYYKTWLHSGQ+V VEEK +++D   ++  TIQGLTSSGYLL
Sbjct: 237  FEIFFNLFATQGFKPLEELYYKTWLHSGQRVIVEEKNDEKDHGRQSMVTIQGLTSSGYLL 296

Query: 1055 AIGEDGQMCELHPDGNSFDFFKGLVRRKL 1141
            AIG+D QMCELHPDGNSFDFFKGL+RRKL
Sbjct: 297  AIGDDNQMCELHPDGNSFDFFKGLIRRKL 325


>ref|XP_022856813.1| biotin--protein ligase 2-like isoform X1 [Olea europaea var.
            sylvestris]
          Length = 363

 Score =  513 bits (1321), Expect = e-179
 Identities = 255/355 (71%), Positives = 300/355 (84%), Gaps = 2/355 (0%)
 Frame = +2

Query: 83   LSLSHPHILPETHKKCLLSLSASAMESQSPCVLVLCGKSSTEKELAKSMKNNSILKLPEN 262
            LSL     +P+T    LLS+SAS ME +SP  L+LCGKSSTE ELA  +K N+ LKLPEN
Sbjct: 11   LSLPKLIFIPKT---ILLSVSASKMELESPTTLILCGKSSTENELAMLLKTNNTLKLPEN 67

Query: 263  AKVSVFLHLEKEK--SFDEESFCINSYMNSLSTARFGRFLIWSPQLPSTHDVVSNNFGDL 436
            A ++V LH E EK  SF +++F I+SYMNSLST+RFG+FLI+SP+L STHDVVS+NF +L
Sbjct: 68   ASINVVLHSENEKQESFQKDAFQIDSYMNSLSTSRFGKFLIYSPKLASTHDVVSHNFCEL 127

Query: 437  PIGAVCVADFQYKGRGRSNNVWESPKGCLLFSFTMQMEDGRVVPLVQYVVSLAMTEAIKD 616
            P+GAVCVAD Q KGRGRS NVWESPKGCL+FSFT+QMEDGRVVPLVQYVV LAM EAI D
Sbjct: 128  PLGAVCVADVQLKGRGRSKNVWESPKGCLMFSFTLQMEDGRVVPLVQYVVCLAMAEAIND 187

Query: 617  VCDRNGVPHLDVRIKWPNDLYLNGLKVGGILCTSTYRSKKFNVSAGVGLNVDNEKPTTSL 796
            VC RNG+P  +VRIKWPNDLYL+GLKVGGILCTSTYRSKKF+VSAG+GLNV NEKPTT L
Sbjct: 188  VCLRNGIPPPNVRIKWPNDLYLDGLKVGGILCTSTYRSKKFSVSAGIGLNVSNEKPTTCL 247

Query: 797  NSVLKKLTLAADQLQREDLIAAFFNKFESLFNLFMNQGFQPLEELYYKTWLHSGQKVTVE 976
            N+++++LT    QLQRED++AAFFNKFE+ +++F+ QGFQ LEELYYKTWLHSGQ+V V+
Sbjct: 248  NAIIQRLTSVVYQLQREDIVAAFFNKFENYYDIFICQGFQALEELYYKTWLHSGQRVIVQ 307

Query: 977  EKKEDQDQPVRNEFTIQGLTSSGYLLAIGEDGQMCELHPDGNSFDFFKGLVRRKL 1141
            EK E+QDQ   N  TIQG+T+SGYLLA+ EDGQ CELHPDGNSFDFFKGLVRRKL
Sbjct: 308  EKNENQDQISENVVTIQGITTSGYLLAVTEDGQACELHPDGNSFDFFKGLVRRKL 362


>ref|XP_015902016.1| PREDICTED: biotin--protein ligase 2-like [Ziziphus jujuba]
          Length = 437

 Score =  516 bits (1328), Expect = e-179
 Identities = 249/336 (74%), Positives = 292/336 (86%), Gaps = 1/336 (0%)
 Frame = +2

Query: 137  SLSASAMESQSPCVLVLCGKSSTEKELAKSMKNNSILKLPENAKVSVFLHLEKEKSFDE- 313
            S S+S+  S  PC LVLCGKSS E E+A+S+KNN+ LKLP+N  VSV L  E +K  D+ 
Sbjct: 103  SSSSSSSSSSPPCKLVLCGKSSAENEIAESLKNNNTLKLPDNDAVSVLLQSEWDKPLDDG 162

Query: 314  ESFCINSYMNSLSTARFGRFLIWSPQLPSTHDVVSNNFGDLPIGAVCVADFQYKGRGRSN 493
            +SF I+S+MNSLST RFGRFL+WSP L STHDVVS+NFG+LP+GAVCVAD QYKGRGRS 
Sbjct: 163  DSFRIDSFMNSLSTNRFGRFLLWSPLLTSTHDVVSHNFGELPVGAVCVADVQYKGRGRSK 222

Query: 494  NVWESPKGCLLFSFTMQMEDGRVVPLVQYVVSLAMTEAIKDVCDRNGVPHLDVRIKWPND 673
            NVW+SPKGCLLFSFT+QMEDGRVVPLVQYVVSLA+TEAIKD+CD+NG+P++DV+IKWPND
Sbjct: 223  NVWQSPKGCLLFSFTLQMEDGRVVPLVQYVVSLAVTEAIKDICDKNGLPYVDVKIKWPND 282

Query: 674  LYLNGLKVGGILCTSTYRSKKFNVSAGVGLNVDNEKPTTSLNSVLKKLTLAADQLQREDL 853
            LYLN LKVGGILCTSTY+SKKFN+SAG+GLNVDNEKPT  LN +L++L++ A Q +RED+
Sbjct: 283  LYLNNLKVGGILCTSTYKSKKFNISAGIGLNVDNEKPTICLNGLLRELSVVAYQFEREDI 342

Query: 854  IAAFFNKFESLFNLFMNQGFQPLEELYYKTWLHSGQKVTVEEKKEDQDQPVRNEFTIQGL 1033
            +A FFNKFE  ++LFM+QGFQ LEELYYKTWLHSGQ+V V+EK E  DQ V N  T+QGL
Sbjct: 343  LACFFNKFEIFYDLFMDQGFQSLEELYYKTWLHSGQRVIVQEKNE--DQVVENVVTVQGL 400

Query: 1034 TSSGYLLAIGEDGQMCELHPDGNSFDFFKGLVRRKL 1141
            TSSGYLLAIG+D QMCELHPDGNSFDFFKGLVRRKL
Sbjct: 401  TSSGYLLAIGDDNQMCELHPDGNSFDFFKGLVRRKL 436


>ref|XP_008233907.1| PREDICTED: biotin--protein ligase 2 [Prunus mume]
          Length = 365

 Score =  512 bits (1319), Expect = e-178
 Identities = 243/343 (70%), Positives = 294/343 (85%)
 Frame = +2

Query: 113  ETHKKCLLSLSASAMESQSPCVLVLCGKSSTEKELAKSMKNNSILKLPENAKVSVFLHLE 292
            +T+    L ++ASAM+  S C LVLCGKSS E E AK++KN+  LKL +N ++S+ LH E
Sbjct: 24   KTNSSLSLPVAASAMDGNSSCALVLCGKSSAENETAKTLKNSDALKLTDNTELSILLHSE 83

Query: 293  KEKSFDEESFCINSYMNSLSTARFGRFLIWSPQLPSTHDVVSNNFGDLPIGAVCVADFQY 472
              +   E++F ++S+M+SLST RFGRFL+WSP+LPSTHDVVS NF +LPIGAVCVAD QY
Sbjct: 84   SAEPIKEDAFGVDSFMSSLSTNRFGRFLLWSPRLPSTHDVVSQNFCELPIGAVCVADVQY 143

Query: 473  KGRGRSNNVWESPKGCLLFSFTMQMEDGRVVPLVQYVVSLAMTEAIKDVCDRNGVPHLDV 652
            KGRGRS NVWESPKGCLLFSFT+QMEDGRVVPL+QYVVSLA+TEAIKDVCD+NG+P++DV
Sbjct: 144  KGRGRSKNVWESPKGCLLFSFTLQMEDGRVVPLIQYVVSLAVTEAIKDVCDKNGLPYVDV 203

Query: 653  RIKWPNDLYLNGLKVGGILCTSTYRSKKFNVSAGVGLNVDNEKPTTSLNSVLKKLTLAAD 832
            +IKWPNDLYLNGLKVGGILCTST++SK+FNVSAG+GLNVDNEKPTT LN+ L++L++   
Sbjct: 204  KIKWPNDLYLNGLKVGGILCTSTFKSKQFNVSAGIGLNVDNEKPTTCLNTFLRELSVTTY 263

Query: 833  QLQREDLIAAFFNKFESLFNLFMNQGFQPLEELYYKTWLHSGQKVTVEEKKEDQDQPVRN 1012
            Q +RED++AAFF+KFE  + LF+NQGFQ LEELYY+TWLHSGQ+V V+E  +  DQ V N
Sbjct: 264  QFRREDILAAFFDKFEKFYELFINQGFQSLEELYYQTWLHSGQRVIVQEMND--DQVVEN 321

Query: 1013 EFTIQGLTSSGYLLAIGEDGQMCELHPDGNSFDFFKGLVRRKL 1141
              T+QGLTSSGYLLAIG+D QMCELHPDGNSFDFFKGLVRRKL
Sbjct: 322  VVTVQGLTSSGYLLAIGDDNQMCELHPDGNSFDFFKGLVRRKL 364


>ref|XP_022856814.1| biotin--protein ligase 2-like isoform X2 [Olea europaea var.
            sylvestris]
          Length = 363

 Score =  511 bits (1315), Expect = e-178
 Identities = 254/355 (71%), Positives = 299/355 (84%), Gaps = 2/355 (0%)
 Frame = +2

Query: 83   LSLSHPHILPETHKKCLLSLSASAMESQSPCVLVLCGKSSTEKELAKSMKNNSILKLPEN 262
            LSL     +P+T     LS+SAS ME +SP  L+LCGKSSTE ELA  +K N+ LKLPEN
Sbjct: 11   LSLPKLIFIPKTIS---LSVSASKMELESPTTLILCGKSSTENELAMLLKTNNTLKLPEN 67

Query: 263  AKVSVFLHLEKEK--SFDEESFCINSYMNSLSTARFGRFLIWSPQLPSTHDVVSNNFGDL 436
            A ++V LH E EK  SF +++F I+SYMNSLST+RFG+FLI+SP+L STHDVVS+NF +L
Sbjct: 68   ASINVVLHSENEKQESFQKDAFQIDSYMNSLSTSRFGKFLIYSPKLASTHDVVSHNFCEL 127

Query: 437  PIGAVCVADFQYKGRGRSNNVWESPKGCLLFSFTMQMEDGRVVPLVQYVVSLAMTEAIKD 616
            P+GAVCVAD Q KGRGRS NVWESPKGCL+FSFT+QMEDGRVVPLVQYVV LAM EAI D
Sbjct: 128  PLGAVCVADVQLKGRGRSKNVWESPKGCLMFSFTLQMEDGRVVPLVQYVVCLAMAEAIND 187

Query: 617  VCDRNGVPHLDVRIKWPNDLYLNGLKVGGILCTSTYRSKKFNVSAGVGLNVDNEKPTTSL 796
            VC RNG+P  +VRIKWPNDLYL+GLKVGGILCTSTYRSKKF+VSAG+GLNV NEKPTT L
Sbjct: 188  VCLRNGIPPPNVRIKWPNDLYLDGLKVGGILCTSTYRSKKFSVSAGIGLNVSNEKPTTCL 247

Query: 797  NSVLKKLTLAADQLQREDLIAAFFNKFESLFNLFMNQGFQPLEELYYKTWLHSGQKVTVE 976
            N+++++LT    QLQRED++AAFFNKFE+ +++F+ QGFQ LEELYYKTWLHSGQ+V V+
Sbjct: 248  NAIIQRLTSVVYQLQREDIVAAFFNKFENYYDIFICQGFQALEELYYKTWLHSGQRVIVQ 307

Query: 977  EKKEDQDQPVRNEFTIQGLTSSGYLLAIGEDGQMCELHPDGNSFDFFKGLVRRKL 1141
            EK E+QDQ   N  TIQG+T+SGYLLA+ EDGQ CELHPDGNSFDFFKGLVRRKL
Sbjct: 308  EKNENQDQISENVVTIQGITTSGYLLAVTEDGQACELHPDGNSFDFFKGLVRRKL 362


>gb|EOY16190.1| Holocarboxylase synthase 1 isoform 1 [Theobroma cacao]
          Length = 375

 Score =  509 bits (1310), Expect = e-177
 Identities = 255/372 (68%), Positives = 306/372 (82%), Gaps = 3/372 (0%)
 Frame = +2

Query: 35   LSKKFPILSLSKKFPILSLSHPHILPETHKKCLLSLSASAMESQSPCVLVLCGKSSTEKE 214
            +S +F +LS    F +LSL +      T  +   S++ASAME+ SP  L LCGKSS+E E
Sbjct: 12   VSSRFRLLSPHISFSVLSLKN-----HTPSRLSFSVAASAMETSSPSALFLCGKSSSETE 66

Query: 215  LAKSMKNNSILKLPENAKVSVFLHLEKEKS---FDEESFCINSYMNSLSTARFGRFLIWS 385
            +AKS+K N+ LKLP   +VS   HL+ E S     EE+F +N ++NSLST RFGR LIWS
Sbjct: 67   IAKSLKINNALKLPNGGEVST--HLQSEISNFQLGEEAFNVNLFLNSLSTNRFGRNLIWS 124

Query: 386  PQLPSTHDVVSNNFGDLPIGAVCVADFQYKGRGRSNNVWESPKGCLLFSFTMQMEDGRVV 565
            P+LPSTHDVVS+NF +LPIGA CVAD Q+KGRGRS N WESP+GCL+FSFT++MEDG++V
Sbjct: 125  PRLPSTHDVVSHNFCELPIGAACVADVQFKGRGRSKNAWESPRGCLMFSFTLEMEDGKIV 184

Query: 566  PLVQYVVSLAMTEAIKDVCDRNGVPHLDVRIKWPNDLYLNGLKVGGILCTSTYRSKKFNV 745
            PLVQYVVSLA+TEAIKD+CDR G P LDVRIKWPNDLYLNGLKVGGILCTSTY+SKKFNV
Sbjct: 185  PLVQYVVSLALTEAIKDICDREGFPCLDVRIKWPNDLYLNGLKVGGILCTSTYKSKKFNV 244

Query: 746  SAGVGLNVDNEKPTTSLNSVLKKLTLAADQLQREDLIAAFFNKFESLFNLFMNQGFQPLE 925
            S+GVGLNVDNEKPTT LN+VLK+L+  +++ +RED++AAFFNKFE L+++F+NQGFQ LE
Sbjct: 245  SSGVGLNVDNEKPTTCLNAVLKELSTTSNKFRREDIMAAFFNKFEMLYDIFLNQGFQTLE 304

Query: 926  ELYYKTWLHSGQKVTVEEKKEDQDQPVRNEFTIQGLTSSGYLLAIGEDGQMCELHPDGNS 1105
            ELYY+TWLHSGQ+V V+EK E  D+ V N  TIQGLTSSGYLLAIG+D QMCELHPDGNS
Sbjct: 305  ELYYRTWLHSGQRVIVQEKNE--DRIVENVVTIQGLTSSGYLLAIGDDYQMCELHPDGNS 362

Query: 1106 FDFFKGLVRRKL 1141
            FDFFKGLVRRKL
Sbjct: 363  FDFFKGLVRRKL 374


>ref|XP_023926082.1| biotin--protein ligase 2-like isoform X1 [Quercus suber]
 gb|POE93725.1| biotin--protein ligase 1, chloroplastic [Quercus suber]
          Length = 367

 Score =  508 bits (1307), Expect = e-177
 Identities = 263/378 (69%), Positives = 306/378 (80%)
 Frame = +2

Query: 8    LSLRPTLRFLSKKFPILSLSKKFPILSLSHPHILPETHKKCLLSLSASAMESQSPCVLVL 187
            +S++ +LRFL+ +  I SL     ILS   P  L   +K     LSA  M+S S   LVL
Sbjct: 1    MSVKNSLRFLTLRNSIHSL-----ILS---PRKLTFPYKLSFSPLSA--MDSNSSSTLVL 50

Query: 188  CGKSSTEKELAKSMKNNSILKLPENAKVSVFLHLEKEKSFDEESFCINSYMNSLSTARFG 367
            CGKS+ E E+A S+K+N  LKLP +++VSV L  E EK  +E SF I+SYM SLST++FG
Sbjct: 51   CGKSTAENEIALSLKSNKTLKLPGDSEVSVLLKSELEKPLEENSFRIDSYMGSLSTSQFG 110

Query: 368  RFLIWSPQLPSTHDVVSNNFGDLPIGAVCVADFQYKGRGRSNNVWESPKGCLLFSFTMQM 547
            RFLIWSP+LPSTHDVVS+NFG+LP G VCVAD Q+KGRGRS NVWESP GCL+FSFTMQM
Sbjct: 111  RFLIWSPRLPSTHDVVSHNFGELPFGTVCVADGQFKGRGRSKNVWESPMGCLMFSFTMQM 170

Query: 548  EDGRVVPLVQYVVSLAMTEAIKDVCDRNGVPHLDVRIKWPNDLYLNGLKVGGILCTSTYR 727
            EDGRVVPLVQYVVSLA+TEAIKDVCD+NG+P +DV+IKWPNDLYLNGLKVGGILCTSTY 
Sbjct: 171  EDGRVVPLVQYVVSLAITEAIKDVCDKNGLPCIDVKIKWPNDLYLNGLKVGGILCTSTYG 230

Query: 728  SKKFNVSAGVGLNVDNEKPTTSLNSVLKKLTLAADQLQREDLIAAFFNKFESLFNLFMNQ 907
            SKKFNVSAG+GLNVDNEKPTT LN+VLK+L++ A QL RE+++ AFF KFE   +LF+NQ
Sbjct: 231  SKKFNVSAGIGLNVDNEKPTTCLNAVLKELSVVAYQLSREEILVAFFEKFEKFNDLFINQ 290

Query: 908  GFQPLEELYYKTWLHSGQKVTVEEKKEDQDQPVRNEFTIQGLTSSGYLLAIGEDGQMCEL 1087
            GFQ LEELYYKTWLHSGQ+V V+EK E  DQ V N  TIQGLTSSGYLLAIG+D QM EL
Sbjct: 291  GFQTLEELYYKTWLHSGQRVIVQEKNE--DQMVENVVTIQGLTSSGYLLAIGDDNQMYEL 348

Query: 1088 HPDGNSFDFFKGLVRRKL 1141
            HPDGNS DFFKGL+RRKL
Sbjct: 349  HPDGNSLDFFKGLIRRKL 366


>ref|XP_007018965.2| PREDICTED: biotin--protein ligase 2 isoform X3 [Theobroma cacao]
          Length = 375

 Score =  507 bits (1306), Expect = e-176
 Identities = 255/372 (68%), Positives = 304/372 (81%), Gaps = 3/372 (0%)
 Frame = +2

Query: 35   LSKKFPILSLSKKFPILSLSHPHILPETHKKCLLSLSASAMESQSPCVLVLCGKSSTEKE 214
            +S +F +LS    F  LSL +      T  +   S++ASAME+ SP  L LCGKSS E E
Sbjct: 12   VSSRFRLLSPHISFSFLSLKN-----HTPSRLSFSVAASAMETSSPSALFLCGKSSAETE 66

Query: 215  LAKSMKNNSILKLPENAKVSVFLHLEKEKS---FDEESFCINSYMNSLSTARFGRFLIWS 385
            +AKS+K N+ LKLP   +VS   HL+ E S     EE+F +N ++NSLST RFGR LIWS
Sbjct: 67   IAKSLKINNALKLPNGGEVST--HLQSEISNFQLGEEAFNVNLFLNSLSTNRFGRNLIWS 124

Query: 386  PQLPSTHDVVSNNFGDLPIGAVCVADFQYKGRGRSNNVWESPKGCLLFSFTMQMEDGRVV 565
            P+LPSTHDVVS+NF +LPIGA CVAD Q+KGRGRS N WESP+GCL+FSFT++MEDG++V
Sbjct: 125  PRLPSTHDVVSHNFCELPIGAACVADVQFKGRGRSKNAWESPRGCLMFSFTLEMEDGKIV 184

Query: 566  PLVQYVVSLAMTEAIKDVCDRNGVPHLDVRIKWPNDLYLNGLKVGGILCTSTYRSKKFNV 745
            PLVQYVVSLA+TEAIKD+CDR G P LDVRIKWPNDLYLNGLKVGGILCTSTY+SKKFNV
Sbjct: 185  PLVQYVVSLALTEAIKDICDREGFPCLDVRIKWPNDLYLNGLKVGGILCTSTYKSKKFNV 244

Query: 746  SAGVGLNVDNEKPTTSLNSVLKKLTLAADQLQREDLIAAFFNKFESLFNLFMNQGFQPLE 925
            S+GVGLNVDNEKPTT LN+VLK+L+  +++ +RED++AAFFNKFE L+++F+NQGFQ LE
Sbjct: 245  SSGVGLNVDNEKPTTCLNAVLKELSTTSNKFRREDIMAAFFNKFEMLYDIFLNQGFQTLE 304

Query: 926  ELYYKTWLHSGQKVTVEEKKEDQDQPVRNEFTIQGLTSSGYLLAIGEDGQMCELHPDGNS 1105
            ELYY+TWLHSGQ+V V+EK E  D+ V N  TIQGLTSSGYLLAIG+D QMCELHPDGNS
Sbjct: 305  ELYYRTWLHSGQRVIVQEKNE--DRIVENVVTIQGLTSSGYLLAIGDDYQMCELHPDGNS 362

Query: 1106 FDFFKGLVRRKL 1141
            FDFFKGLVRRKL
Sbjct: 363  FDFFKGLVRRKL 374


>ref|XP_002307419.1| holocarboxylase synthetase 2 family protein [Populus trichocarpa]
 gb|PNT37159.1| hypothetical protein POPTR_005G173000v3 [Populus trichocarpa]
          Length = 331

 Score =  504 bits (1298), Expect = e-176
 Identities = 242/329 (73%), Positives = 283/329 (86%), Gaps = 2/329 (0%)
 Frame = +2

Query: 161  SQSPCVLVLCGKSSTEKELAKSMKNNSILKLPENAKVSVFLHLE--KEKSFDEESFCINS 334
            S +PC L+LCGKSS E E+AKS+ NN+ LKLP+N ++S   H E   E   +EESF ++ 
Sbjct: 4    SSTPCKLLLCGKSSAENEIAKSLMNNNTLKLPDNVEISTLSHSEILDEPQQNEESFSLSR 63

Query: 335  YMNSLSTARFGRFLIWSPQLPSTHDVVSNNFGDLPIGAVCVADFQYKGRGRSNNVWESPK 514
            +MNSLST  FGR LIWSP LPSTHD+VSNNFG+LPIG VC+AD QYKGRGRS NVWESP 
Sbjct: 64   FMNSLSTNLFGRLLIWSPLLPSTHDLVSNNFGELPIGTVCIADVQYKGRGRSKNVWESPA 123

Query: 515  GCLLFSFTMQMEDGRVVPLVQYVVSLAMTEAIKDVCDRNGVPHLDVRIKWPNDLYLNGLK 694
            GCL+FSFT+QMEDG+VVPL+QYVVSLA+TEAIKD+CD+NG+PH+DVRIKWPNDLYLNG+K
Sbjct: 124  GCLMFSFTIQMEDGQVVPLLQYVVSLAVTEAIKDLCDKNGLPHIDVRIKWPNDLYLNGVK 183

Query: 695  VGGILCTSTYRSKKFNVSAGVGLNVDNEKPTTSLNSVLKKLTLAADQLQREDLIAAFFNK 874
            VGGILCTSTYRSKKFN+SAG+GLNVDNEKPTT LN+VL++L  AA  L+RED++AAF NK
Sbjct: 184  VGGILCTSTYRSKKFNISAGIGLNVDNEKPTTCLNAVLRELAGAACALRREDIVAAFLNK 243

Query: 875  FESLFNLFMNQGFQPLEELYYKTWLHSGQKVTVEEKKEDQDQPVRNEFTIQGLTSSGYLL 1054
            FE L++LF+N GFQ LEELYYKTWLHSGQ+V ++EK E  +Q V N  TIQGLT SGYLL
Sbjct: 244  FEKLYDLFINGGFQTLEELYYKTWLHSGQRVIIQEKNE--NQVVENVVTIQGLTPSGYLL 301

Query: 1055 AIGEDGQMCELHPDGNSFDFFKGLVRRKL 1141
            AIGED QMCELHPDGNSFDFFKGLVRRK+
Sbjct: 302  AIGEDNQMCELHPDGNSFDFFKGLVRRKI 330


>ref|XP_012078166.1| biotin--protein ligase 2 [Jatropha curcas]
 gb|KDP32755.1| hypothetical protein JCGZ_12047 [Jatropha curcas]
          Length = 367

 Score =  504 bits (1299), Expect = e-175
 Identities = 256/368 (69%), Positives = 306/368 (83%), Gaps = 2/368 (0%)
 Frame = +2

Query: 44   KFPILSLSKKFPILSLSHPHILPETHKK-CLLSLSASAMES-QSPCVLVLCGKSSTEKEL 217
            KF  LSL   + ++  +       T+K+  +L  S +AM+S +S C LVL GKSS EKE+
Sbjct: 8    KFTSLSLRSLYKLIPAT------ATYKRGFVLYRSIAAMDSSRSSCTLVLSGKSSAEKEI 61

Query: 218  AKSMKNNSILKLPENAKVSVFLHLEKEKSFDEESFCINSYMNSLSTARFGRFLIWSPQLP 397
            A+S+K N +LKLP+N  VS+ +  E  K  ++E+F +  + N LSTARFGRFLIWSP+L 
Sbjct: 62   AQSLKKNGVLKLPDNTVVSMLMESEIAKP-EKENFNLELFFNCLSTARFGRFLIWSPRLT 120

Query: 398  STHDVVSNNFGDLPIGAVCVADFQYKGRGRSNNVWESPKGCLLFSFTMQMEDGRVVPLVQ 577
            STHDVVS+NF +LP+G VCVAD QYKGRGRS NVWESP GCLLFSFT+QMEDGRVVPL+Q
Sbjct: 121  STHDVVSHNFCELPVGTVCVADVQYKGRGRSKNVWESPPGCLLFSFTVQMEDGRVVPLLQ 180

Query: 578  YVVSLAMTEAIKDVCDRNGVPHLDVRIKWPNDLYLNGLKVGGILCTSTYRSKKFNVSAGV 757
            YVVSLA+TEAIKDVC +NG+P LDV+IKWPNDLYLNGLKVGGILCTSTY+SK FNVSAGV
Sbjct: 181  YVVSLAVTEAIKDVCHKNGLPFLDVKIKWPNDLYLNGLKVGGILCTSTYKSKTFNVSAGV 240

Query: 758  GLNVDNEKPTTSLNSVLKKLTLAADQLQREDLIAAFFNKFESLFNLFMNQGFQPLEELYY 937
            GLNVDNEKPTT LN+VL++L+ AAD+L+RED++AAFFNK+E+LF+LF+NQGFQ LEELYY
Sbjct: 241  GLNVDNEKPTTCLNAVLRELSTAADRLRREDIVAAFFNKYENLFDLFINQGFQTLEELYY 300

Query: 938  KTWLHSGQKVTVEEKKEDQDQPVRNEFTIQGLTSSGYLLAIGEDGQMCELHPDGNSFDFF 1117
            +TWLHSGQ+V ++EK  + DQ V N  TIQGLTSSGYLLAIGED QMCELHPDGNSFDFF
Sbjct: 301  RTWLHSGQRVIIQEK--NGDQIVENAVTIQGLTSSGYLLAIGEDDQMCELHPDGNSFDFF 358

Query: 1118 KGLVRRKL 1141
            KGLVRRKL
Sbjct: 359  KGLVRRKL 366


>ref|XP_009366988.1| PREDICTED: biotin--protein ligase 2-like [Pyrus x bretschneideri]
          Length = 368

 Score =  502 bits (1293), Expect = e-174
 Identities = 238/337 (70%), Positives = 290/337 (86%)
 Frame = +2

Query: 131  LLSLSASAMESQSPCVLVLCGKSSTEKELAKSMKNNSILKLPENAKVSVFLHLEKEKSFD 310
            L + +A AM+    C LVLCGKS  E E AK++KN++ L LPE+ + S+FL  E EK   
Sbjct: 33   LPAAAALAMDCDPNCTLVLCGKSPAENEAAKALKNSNALNLPESTEFSIFLQSESEKPVK 92

Query: 311  EESFCINSYMNSLSTARFGRFLIWSPQLPSTHDVVSNNFGDLPIGAVCVADFQYKGRGRS 490
            E++F ++S+M+SLST+RFGR L+WSP+LPSTHDVVS NF +LP+G VCVAD QYKGRGRS
Sbjct: 93   EDAFGVDSFMSSLSTSRFGRLLLWSPRLPSTHDVVSKNFCELPVGTVCVADVQYKGRGRS 152

Query: 491  NNVWESPKGCLLFSFTMQMEDGRVVPLVQYVVSLAMTEAIKDVCDRNGVPHLDVRIKWPN 670
             NVWESP+GCLLFSFT+QME+GRVVPL+QYVVSLA+TEAIKDVCD+NG+P++D++IKWPN
Sbjct: 153  KNVWESPEGCLLFSFTLQMENGRVVPLIQYVVSLAVTEAIKDVCDKNGLPYVDLKIKWPN 212

Query: 671  DLYLNGLKVGGILCTSTYRSKKFNVSAGVGLNVDNEKPTTSLNSVLKKLTLAADQLQRED 850
            DLYLNGLKVGGILCTSTY+SK+FNVSAG+GLNVDNEKPTT LN+ L++L++   + +RED
Sbjct: 213  DLYLNGLKVGGILCTSTYKSKQFNVSAGIGLNVDNEKPTTCLNAFLRELSVTTYKFRRED 272

Query: 851  LIAAFFNKFESLFNLFMNQGFQPLEELYYKTWLHSGQKVTVEEKKEDQDQPVRNEFTIQG 1030
            ++AAFFNKFE L+ +F+NQGFQ LEE+YY+TWLHSGQ+V V+EK E  DQ V N  TIQG
Sbjct: 273  VLAAFFNKFEMLYEVFINQGFQSLEEVYYRTWLHSGQRVIVQEKSE--DQVVENVVTIQG 330

Query: 1031 LTSSGYLLAIGEDGQMCELHPDGNSFDFFKGLVRRKL 1141
            LTSSGYLLAIG+D QMCELHPDGNSFDFFKGLVRRKL
Sbjct: 331  LTSSGYLLAIGDDNQMCELHPDGNSFDFFKGLVRRKL 367