BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Acanthopanax21_contig00020094
         (1602 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|KZN11953.1| hypothetical protein DCAR_004609 [Daucus carota s...   295   2e-85
ref|XP_017230627.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   295   2e-85
ref|XP_017237104.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   276   2e-79
gb|KZN07967.1| hypothetical protein DCAR_000636 [Daucus carota s...   274   4e-79
ref|XP_017237143.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   274   9e-79
ref|XP_019071879.1| PREDICTED: E4 SUMO-protein ligase PIAL1 isof...   220   5e-59
emb|CAN82727.1| hypothetical protein VITISV_040324 [Vitis vinifera]   220   5e-59
ref|XP_010658435.1| PREDICTED: E4 SUMO-protein ligase PIAL1 isof...   220   6e-59
ref|XP_023911115.1| E4 SUMO-protein ligase PIAL2-like isoform X2...   216   1e-57
ref|XP_023911114.1| E4 SUMO-protein ligase PIAL2-like isoform X1...   216   2e-57
ref|XP_017192140.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   200   7e-53
ref|XP_022737523.1| E4 SUMO-protein ligase PIAL2-like isoform X3...   200   6e-52
ref|XP_008348548.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   200   6e-52
ref|XP_022737522.1| E4 SUMO-protein ligase PIAL2-like isoform X2...   200   7e-52
ref|XP_022737521.1| E4 SUMO-protein ligase PIAL2-like isoform X1...   200   7e-52
ref|XP_021820820.1| E4 SUMO-protein ligase PIAL2 isoform X2 [Pru...   197   5e-51
ref|XP_021820818.1| E4 SUMO-protein ligase PIAL2 isoform X1 [Pru...   197   5e-51
gb|EOY24861.1| RING/U-box superfamily protein, putative [Theobro...   195   5e-50
ref|XP_017973228.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   194   7e-50
ref|XP_007040360.2| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   194   7e-50

>gb|KZN11953.1| hypothetical protein DCAR_004609 [Daucus carota subsp. sativus]
          Length = 1030

 Score =  295 bits (754), Expect = 2e-85
 Identities = 180/360 (50%), Positives = 217/360 (60%)
 Frame = +1

Query: 28   QSGGVSESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAPGSTPLQQFG 207
            Q+ G++ SAP N+MLSPVLTDA+SPALNREPE +  + LATSV  S  +AP +T LQ   
Sbjct: 485  QANGITGSAPTNYMLSPVLTDAVSPALNREPEGIPASTLATSVSPSHNAAPFNTQLQH-- 542

Query: 208  TCNMINEYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSRMASQPSPTPLMTDGSSTA 387
              +M NEYGR P  S  +NR PVAVQALPAQ  TS  QQR    SQ SP P+M +GS  +
Sbjct: 543  -ADMTNEYGRYPPTSQTLNRVPVAVQALPAQASTSVSQQRPMSTSQMSPKPVMVNGSGFS 601

Query: 388  FSTVERQQQYRSHLNXXXXXXXXXXXXXXXHIGNWGPHGHLFSSGRPSQQINGLPSQQIH 567
             S VE QQQ R                    IG+WGP GHLFS  RPSQQ+         
Sbjct: 602  -SNVEGQQQPR-----PLQGSYMSSSTLQQQIGSWGPQGHLFSPSRPSQQM--------- 646

Query: 568  TRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRMPHLRTQSPSPNRSSSHLPQSQTNQ 747
               +    P GYRV      + GQN HQ     QRMPH+R+QSP   R+  HLP +QT+ 
Sbjct: 647  ---STPLAPGGYRVP----FSNGQNSHQQHFTGQRMPHIRSQSPGLARTPPHLPPTQTHH 699

Query: 748  GGLQNRVSLTPVGGYQKDQFLVAAQRASQMARMHSPSPSVQVQTPRTSSSIPVNVDGYRP 927
            GG+ NR S   V   Q++QF   +QR S + R  S    VQ+QT    + +P NVD +RP
Sbjct: 700  GGMPNRASSPYV--RQQEQFHSFSQRTSHLPRTPSLFNPVQLQT----TPMPRNVDAHRP 753

Query: 928  SGGNTRVPVQPVTRPDGLVDSSVAGQDWRPTGRMRGSLSGQAYSAALNQYISLPTQPVQP 1107
            S  NT   +QPV  P+G V++S   QDWRPTGRMRGSLSG+AYS ALNQYI  PTQPVQP
Sbjct: 754  SISNTGGNLQPVGTPEGTVNTSTE-QDWRPTGRMRGSLSGRAYSEALNQYIIQPTQPVQP 812


>ref|XP_017230627.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like [Daucus carota subsp.
            sativus]
          Length = 1031

 Score =  295 bits (754), Expect = 2e-85
 Identities = 180/360 (50%), Positives = 217/360 (60%)
 Frame = +1

Query: 28   QSGGVSESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAPGSTPLQQFG 207
            Q+ G++ SAP N+MLSPVLTDA+SPALNREPE +  + LATSV  S  +AP +T LQ   
Sbjct: 486  QANGITGSAPTNYMLSPVLTDAVSPALNREPEGIPASTLATSVSPSHNAAPFNTQLQH-- 543

Query: 208  TCNMINEYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSRMASQPSPTPLMTDGSSTA 387
              +M NEYGR P  S  +NR PVAVQALPAQ  TS  QQR    SQ SP P+M +GS  +
Sbjct: 544  -ADMTNEYGRYPPTSQTLNRVPVAVQALPAQASTSVSQQRPMSTSQMSPKPVMVNGSGFS 602

Query: 388  FSTVERQQQYRSHLNXXXXXXXXXXXXXXXHIGNWGPHGHLFSSGRPSQQINGLPSQQIH 567
             S VE QQQ R                    IG+WGP GHLFS  RPSQQ+         
Sbjct: 603  -SNVEGQQQPR-----PLQGSYMSSSTLQQQIGSWGPQGHLFSPSRPSQQM--------- 647

Query: 568  TRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRMPHLRTQSPSPNRSSSHLPQSQTNQ 747
               +    P GYRV      + GQN HQ     QRMPH+R+QSP   R+  HLP +QT+ 
Sbjct: 648  ---STPLAPGGYRVP----FSNGQNSHQQHFTGQRMPHIRSQSPGLARTPPHLPPTQTHH 700

Query: 748  GGLQNRVSLTPVGGYQKDQFLVAAQRASQMARMHSPSPSVQVQTPRTSSSIPVNVDGYRP 927
            GG+ NR S   V   Q++QF   +QR S + R  S    VQ+QT    + +P NVD +RP
Sbjct: 701  GGMPNRASSPYV--RQQEQFHSFSQRTSHLPRTPSLFNPVQLQT----TPMPRNVDAHRP 754

Query: 928  SGGNTRVPVQPVTRPDGLVDSSVAGQDWRPTGRMRGSLSGQAYSAALNQYISLPTQPVQP 1107
            S  NT   +QPV  P+G V++S   QDWRPTGRMRGSLSG+AYS ALNQYI  PTQPVQP
Sbjct: 755  SISNTGGNLQPVGTPEGTVNTSTE-QDWRPTGRMRGSLSGRAYSEALNQYIIQPTQPVQP 813


>ref|XP_017237104.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Daucus carota
            subsp. sativus]
          Length = 885

 Score =  276 bits (705), Expect = 2e-79
 Identities = 176/360 (48%), Positives = 217/360 (60%)
 Frame = +1

Query: 25   AQSGGVSESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAPGSTPLQQF 204
            AQ   VS SAP +H+ SPVLTDAISPAL+REP++     LA S   SQ + P +TP+QQF
Sbjct: 487  AQVNAVSVSAPTSHV-SPVLTDAISPALSREPDSFHAPPLAASDAPSQSALPVNTPVQQF 545

Query: 205  GTCNMINEYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSRMASQPSPTPLMTDGSST 384
               +M+NEY R P+ SIN N    +VQ LP Q   S +QQR   ASQ  P PLM + S+ 
Sbjct: 546  SNFDMVNEYVRFPTTSINTN----SVQGLPVQASPSILQQRPINASQICPNPLMVNSSNP 601

Query: 385  AFSTVERQQQYRSHLNXXXXXXXXXXXXXXXHIGNWGPHGHLFSSGRPSQQINGLPSQQI 564
             FS VER+QQ  SHL+                + +WG  G  FS  +PSQ I        
Sbjct: 602  VFSNVERRQQPMSHLSSYPGAYMSLSSLQQ-QVRSWGQQGQ-FSPSQPSQPI-------- 651

Query: 565  HTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRMPHLRTQSPSPNRSSSHLPQSQTN 744
             T    S+HP GYRV P V  T+G NLHQ Q+I QRM ++R+ SPS  RSS H P +Q+ 
Sbjct: 652  -TPTVSSRHPRGYRV-PTVPVTDGHNLHQ-QSIGQRMLNIRSPSPSLVRSSVHGPPTQSP 708

Query: 745  QGGLQNRVSLTPVGGYQKDQFLVAAQRASQMARMHSPSPSVQVQTPRTSSSIPVNVDGYR 924
             GGL NRV+ TP  G Q  Q   +AQ+ASQ+A + S   S  VQ P    ++  +    R
Sbjct: 709  HGGLLNRVTSTPPVGQQPGQSHRSAQQASQLAGISSQMLSGLVQEPPIIRNVSADS---R 765

Query: 925  PSGGNTRVPVQPVTRPDGLVDSSVAGQDWRPTGRMRGSLSGQAYSAALNQYISLPTQPVQ 1104
            P  GNT   +QP T P+ LVD S AGQ+WRPTGRMRGSLSG AYSAALNQ+I+ P QPVQ
Sbjct: 766  PLTGNTGGTIQPAT-PEVLVDMSAAGQEWRPTGRMRGSLSGAAYSAALNQFITQPAQPVQ 824


>gb|KZN07967.1| hypothetical protein DCAR_000636 [Daucus carota subsp. sativus]
          Length = 840

 Score =  274 bits (701), Expect = 4e-79
 Identities = 176/360 (48%), Positives = 216/360 (60%)
 Frame = +1

Query: 25   AQSGGVSESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAPGSTPLQQF 204
            AQ   VS SAP +H+ SPVLTDAISPAL+REP++     LA S   SQ + P +TP+QQF
Sbjct: 444  AQVNAVSVSAPTSHV-SPVLTDAISPALSREPDSFHAPPLAASDAPSQSALPVNTPVQQF 502

Query: 205  GTCNMINEYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSRMASQPSPTPLMTDGSST 384
               +M+NEY R P+ SIN N    +VQ LP Q   S +QQR   ASQ  P PLM + S+ 
Sbjct: 503  SNFDMVNEYVRFPTTSINTN----SVQGLPVQASPSILQQRPINASQICPNPLMVNSSNP 558

Query: 385  AFSTVERQQQYRSHLNXXXXXXXXXXXXXXXHIGNWGPHGHLFSSGRPSQQINGLPSQQI 564
             FS VER+QQ  SHL+                  +WG  G  FS  +PSQ I        
Sbjct: 559  VFSNVERRQQPMSHLSSYPGAYMSLSSLQQQ---SWGQQGQ-FSPSQPSQPI-------- 606

Query: 565  HTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRMPHLRTQSPSPNRSSSHLPQSQTN 744
             T    S+HP GYRV P V  T+G NLHQ Q+I QRM ++R+ SPS  RSS H P +Q+ 
Sbjct: 607  -TPTVSSRHPRGYRV-PTVPVTDGHNLHQ-QSIGQRMLNIRSPSPSLVRSSVHGPPTQSP 663

Query: 745  QGGLQNRVSLTPVGGYQKDQFLVAAQRASQMARMHSPSPSVQVQTPRTSSSIPVNVDGYR 924
             GGL NRV+ TP  G Q  Q   +AQ+ASQ+A + S   S  VQ P    ++  +    R
Sbjct: 664  HGGLLNRVTSTPPVGQQPGQSHRSAQQASQLAGISSQMLSGLVQEPPIIRNVSADS---R 720

Query: 925  PSGGNTRVPVQPVTRPDGLVDSSVAGQDWRPTGRMRGSLSGQAYSAALNQYISLPTQPVQ 1104
            P  GNT   +QP T P+ LVD S AGQ+WRPTGRMRGSLSG AYSAALNQ+I+ P QPVQ
Sbjct: 721  PLTGNTGGTIQPAT-PEVLVDMSAAGQEWRPTGRMRGSLSGAAYSAALNQFITQPAQPVQ 779


>ref|XP_017237143.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X2 [Daucus carota
            subsp. sativus]
          Length = 883

 Score =  274 bits (701), Expect = 9e-79
 Identities = 176/360 (48%), Positives = 216/360 (60%)
 Frame = +1

Query: 25   AQSGGVSESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAPGSTPLQQF 204
            AQ   VS SAP +H+ SPVLTDAISPAL+REP++     LA S   SQ + P +TP+QQF
Sbjct: 487  AQVNAVSVSAPTSHV-SPVLTDAISPALSREPDSFHAPPLAASDAPSQSALPVNTPVQQF 545

Query: 205  GTCNMINEYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSRMASQPSPTPLMTDGSST 384
               +M+NEY R P+ SIN N    +VQ LP Q   S +QQR   ASQ  P PLM + S+ 
Sbjct: 546  SNFDMVNEYVRFPTTSINTN----SVQGLPVQASPSILQQRPINASQICPNPLMVNSSNP 601

Query: 385  AFSTVERQQQYRSHLNXXXXXXXXXXXXXXXHIGNWGPHGHLFSSGRPSQQINGLPSQQI 564
             FS VER+QQ  SHL+                  +WG  G  FS  +PSQ I        
Sbjct: 602  VFSNVERRQQPMSHLSSYPGAYMSLSSLQQQ---SWGQQGQ-FSPSQPSQPI-------- 649

Query: 565  HTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRMPHLRTQSPSPNRSSSHLPQSQTN 744
             T    S+HP GYRV P V  T+G NLHQ Q+I QRM ++R+ SPS  RSS H P +Q+ 
Sbjct: 650  -TPTVSSRHPRGYRV-PTVPVTDGHNLHQ-QSIGQRMLNIRSPSPSLVRSSVHGPPTQSP 706

Query: 745  QGGLQNRVSLTPVGGYQKDQFLVAAQRASQMARMHSPSPSVQVQTPRTSSSIPVNVDGYR 924
             GGL NRV+ TP  G Q  Q   +AQ+ASQ+A + S   S  VQ P    ++  +    R
Sbjct: 707  HGGLLNRVTSTPPVGQQPGQSHRSAQQASQLAGISSQMLSGLVQEPPIIRNVSADS---R 763

Query: 925  PSGGNTRVPVQPVTRPDGLVDSSVAGQDWRPTGRMRGSLSGQAYSAALNQYISLPTQPVQ 1104
            P  GNT   +QP T P+ LVD S AGQ+WRPTGRMRGSLSG AYSAALNQ+I+ P QPVQ
Sbjct: 764  PLTGNTGGTIQPAT-PEVLVDMSAAGQEWRPTGRMRGSLSGAAYSAALNQFITQPAQPVQ 822


>ref|XP_019071879.1| PREDICTED: E4 SUMO-protein ligase PIAL1 isoform X2 [Vitis vinifera]
          Length = 898

 Score =  220 bits (561), Expect = 5e-59
 Identities = 177/437 (40%), Positives = 222/437 (50%), Gaps = 19/437 (4%)
 Frame = +1

Query: 7    SGASFLAQSGGVSESAPANHMLSPVLTDAISPALNREPEALR-TTALATSVVQSQFSAPG 183
            S A F+   GG S+ +PAN +L PVLTDAISPALNR  E +R  T L TS +  Q   P 
Sbjct: 498  SDAQFI---GGTSQPSPANFLLPPVLTDAISPALNRGTEDIRGNTHLTTSTLHDQLPIPD 554

Query: 184  STPLQQ--FGTCNMINEYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSRMASQPSPT 357
            S  LQQ  FG   + NEYGR P+I  ++ RTP+AVQALPAQT TS    RSR     +  
Sbjct: 555  SLQLQQAQFGHSIVSNEYGRFPTIPRHITRTPIAVQALPAQTQTSGPHHRSR----TTLI 610

Query: 358  PLMTDGSSTAFSTVERQQQY-RSHLNXXXXXXXXXXXXXXXHIG-NWGPHGHLFSSGRPS 531
             ++ +G +T  S +ER QQ+ RS  N                +  NW            +
Sbjct: 611  SMVPNGPNTVGSDMERPQQFSRSIFNPVQISDISASALQHHSMSQNW------------N 658

Query: 532  QQINGLPSQQIHTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRMPHLRTQSPSPNR 711
            QQ+ G P+     RP     P  YR S   L TE Q L Q Q+     P  RT S     
Sbjct: 659  QQVAGHPTTS--QRPG----PGAYRTSSG-LPTEPQTLQQQQS-----PQARTHSNLLRS 706

Query: 712  SSSHLPQSQTNQGGLQNRVS-LTPVGGYQKDQFLVAAQRASQMARMHSPSPSVQVQTPRT 888
            S+ H  +SQ  QGG Q R +     G  Q  Q +VAAQRA+QM RM  P   VQ QT RT
Sbjct: 707  SAHHHSRSQVQQGGAQGRATHAVGTGISQNAQPMVAAQRAAQMTRMPLP---VQNQTSRT 763

Query: 889  SSSIPVNVDGYRPSG----GNTRVPVQPVTRPDGLVDSSVAGQDWRPTGRMRGSLSGQAY 1056
             S+ PVN +G R +     GN    VQ V+RP+ LVD + + Q+WRPTG MRGSL G+AY
Sbjct: 764  GSAFPVNANGGRSTAGEQRGNIEGMVQAVSRPESLVDLA-SEQNWRPTGLMRGSLVGRAY 822

Query: 1057 SAALNQYISLPTQPVQPAARXXXXXXXXXXXXXXXXHGLMENGRNSHSAQ---------A 1209
            ++ALNQ +  PTQP Q + R                  L+ N R     Q         A
Sbjct: 823  NSALNQLVIQPTQPTQ-STRPPTPITSPPPGFPPHLQALLTNIRTPLVPQAPNYPMTQPA 881

Query: 1210 STAGISGALQEKSSGMN 1260
            ST G SG L E+S G++
Sbjct: 882  STTGGSGILPERSLGLH 898


>emb|CAN82727.1| hypothetical protein VITISV_040324 [Vitis vinifera]
          Length = 901

 Score =  220 bits (561), Expect = 5e-59
 Identities = 177/437 (40%), Positives = 222/437 (50%), Gaps = 19/437 (4%)
 Frame = +1

Query: 7    SGASFLAQSGGVSESAPANHMLSPVLTDAISPALNREPEALR-TTALATSVVQSQFSAPG 183
            S A F+   GG S+ +PAN +L PVLTDAISPALNR  E +R  T L TS +  Q   P 
Sbjct: 501  SDAQFI---GGTSQPSPANFLLPPVLTDAISPALNRGTEDIRGNTHLTTSTLHDQLPIPD 557

Query: 184  STPLQQ--FGTCNMINEYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSRMASQPSPT 357
            S  LQQ  FG   + NEYGR P+I  ++ RTP+AVQALPAQT TS    RSR     +  
Sbjct: 558  SLQLQQAQFGHSIVSNEYGRFPTIPRHITRTPIAVQALPAQTQTSGPHHRSR----TTLI 613

Query: 358  PLMTDGSSTAFSTVERQQQY-RSHLNXXXXXXXXXXXXXXXHIG-NWGPHGHLFSSGRPS 531
             ++ +G +T  S +ER QQ+ RS  N                +  NW            +
Sbjct: 614  SMVPNGPNTVGSDMERPQQFSRSIFNPVQISDISASALQHHSMSQNW------------N 661

Query: 532  QQINGLPSQQIHTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRMPHLRTQSPSPNR 711
            QQ+ G P+     RP     P  YR S   L TE Q L Q Q+     P  RT S     
Sbjct: 662  QQVAGHPTTS--QRPG----PGAYRTSSG-LPTEPQTLQQQQS-----PQARTHSNLLRS 709

Query: 712  SSSHLPQSQTNQGGLQNRVS-LTPVGGYQKDQFLVAAQRASQMARMHSPSPSVQVQTPRT 888
            S+ H  +SQ  QGG Q R +     G  Q  Q +VAAQRA+QM RM  P   VQ QT RT
Sbjct: 710  SAHHHSRSQVQQGGAQGRATHAVGTGISQNAQPMVAAQRAAQMTRMPLP---VQNQTSRT 766

Query: 889  SSSIPVNVDGYRPSG----GNTRVPVQPVTRPDGLVDSSVAGQDWRPTGRMRGSLSGQAY 1056
             S+ PVN +G R +     GN    VQ V+RP+ LVD + + Q+WRPTG MRGSL G+AY
Sbjct: 767  GSAFPVNANGGRSTAGEQRGNIEGMVQAVSRPESLVDLA-SEQNWRPTGLMRGSLVGRAY 825

Query: 1057 SAALNQYISLPTQPVQPAARXXXXXXXXXXXXXXXXHGLMENGRNSHSAQ---------A 1209
            ++ALNQ +  PTQP Q + R                  L+ N R     Q         A
Sbjct: 826  NSALNQLVIQPTQPTQ-STRPPTPITSPPPGFPPHLQALLTNIRTPLVPQAPNYPMTQPA 884

Query: 1210 STAGISGALQEKSSGMN 1260
            ST G SG L E+S G++
Sbjct: 885  STTGGSGILPERSLGLH 901


>ref|XP_010658435.1| PREDICTED: E4 SUMO-protein ligase PIAL1 isoform X1 [Vitis vinifera]
          Length = 922

 Score =  220 bits (561), Expect = 6e-59
 Identities = 177/437 (40%), Positives = 222/437 (50%), Gaps = 19/437 (4%)
 Frame = +1

Query: 7    SGASFLAQSGGVSESAPANHMLSPVLTDAISPALNREPEALR-TTALATSVVQSQFSAPG 183
            S A F+   GG S+ +PAN +L PVLTDAISPALNR  E +R  T L TS +  Q   P 
Sbjct: 522  SDAQFI---GGTSQPSPANFLLPPVLTDAISPALNRGTEDIRGNTHLTTSTLHDQLPIPD 578

Query: 184  STPLQQ--FGTCNMINEYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSRMASQPSPT 357
            S  LQQ  FG   + NEYGR P+I  ++ RTP+AVQALPAQT TS    RSR     +  
Sbjct: 579  SLQLQQAQFGHSIVSNEYGRFPTIPRHITRTPIAVQALPAQTQTSGPHHRSR----TTLI 634

Query: 358  PLMTDGSSTAFSTVERQQQY-RSHLNXXXXXXXXXXXXXXXHIG-NWGPHGHLFSSGRPS 531
             ++ +G +T  S +ER QQ+ RS  N                +  NW            +
Sbjct: 635  SMVPNGPNTVGSDMERPQQFSRSIFNPVQISDISASALQHHSMSQNW------------N 682

Query: 532  QQINGLPSQQIHTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRMPHLRTQSPSPNR 711
            QQ+ G P+     RP     P  YR S   L TE Q L Q Q+     P  RT S     
Sbjct: 683  QQVAGHPTTS--QRPG----PGAYRTSSG-LPTEPQTLQQQQS-----PQARTHSNLLRS 730

Query: 712  SSSHLPQSQTNQGGLQNRVS-LTPVGGYQKDQFLVAAQRASQMARMHSPSPSVQVQTPRT 888
            S+ H  +SQ  QGG Q R +     G  Q  Q +VAAQRA+QM RM  P   VQ QT RT
Sbjct: 731  SAHHHSRSQVQQGGAQGRATHAVGTGISQNAQPMVAAQRAAQMTRMPLP---VQNQTSRT 787

Query: 889  SSSIPVNVDGYRPSG----GNTRVPVQPVTRPDGLVDSSVAGQDWRPTGRMRGSLSGQAY 1056
             S+ PVN +G R +     GN    VQ V+RP+ LVD + + Q+WRPTG MRGSL G+AY
Sbjct: 788  GSAFPVNANGGRSTAGEQRGNIEGMVQAVSRPESLVDLA-SEQNWRPTGLMRGSLVGRAY 846

Query: 1057 SAALNQYISLPTQPVQPAARXXXXXXXXXXXXXXXXHGLMENGRNSHSAQ---------A 1209
            ++ALNQ +  PTQP Q + R                  L+ N R     Q         A
Sbjct: 847  NSALNQLVIQPTQPTQ-STRPPTPITSPPPGFPPHLQALLTNIRTPLVPQAPNYPMTQPA 905

Query: 1210 STAGISGALQEKSSGMN 1260
            ST G SG L E+S G++
Sbjct: 906  STTGGSGILPERSLGLH 922


>ref|XP_023911115.1| E4 SUMO-protein ligase PIAL2-like isoform X2 [Quercus suber]
 gb|POF11949.1| e4 sumo-protein ligase pial2 [Quercus suber]
          Length = 921

 Score =  216 bits (551), Expect = 1e-57
 Identities = 161/412 (39%), Positives = 210/412 (50%), Gaps = 19/412 (4%)
 Frame = +1

Query: 34   GGVSESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAPGSTPLQQFGTC 213
            GG+SE  PAN M SP+LTDA+SPAL+ EPE    T   TS++QSQ S+P +  LQQ    
Sbjct: 513  GGISEPIPANLMQSPLLTDAVSPALSLEPEGHGNTNFTTSLIQSQ-SSPNNFQLQQSQYA 571

Query: 214  NMINEYGRSPSISI---NVNRTPVAVQALPAQTPTSTMQQRSRMASQPS----------P 354
              INEYGR P++     +VNRTP+AVQALPAQ+ T   Q+R R +  P+          P
Sbjct: 572  QSINEYGRLPNLPFIHRHVNRTPIAVQALPAQSQTPNPQERPRSSLTPNGSLISPQVALP 631

Query: 355  TPLMTDGSSTAFSTVERQQQYRSHLNXXXXXXXXXXXXXXXHIGNWGPH-GHLFSSGRPS 531
                 D  +T  S +ERQQ +R+ LN                +     H     +S R  
Sbjct: 632  MAPTADVFNTICSDMERQQHFRAQLNPLQVSDIPSSLLQRPSVIQSRDHQDRSHASSRSH 691

Query: 532  QQINGLPSQQIHTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRMPHLRTQSPSPNR 711
                 +P    HT  A S+  S Y+ +  +L TE QN H  QA + RMP    QS SP  
Sbjct: 692  VSSPSVPPGLGHT--AQSRLQSHYKATSGLL-TEFQNSHLQQAFNPRMPQSVGQSSSPIP 748

Query: 712  SSSHLPQSQTNQGGLQNRVS-LTPVGGYQKDQFLVAAQRASQMARMHSPSPSVQVQTPRT 888
             SSH  ++Q  QGG Q  +S      G Q  ++LV   R        +PS  +  QT RT
Sbjct: 749  PSSHPSRTQVQQGGGQVGISQRAGTTGSQPARYLVNQNRYHPQNSRQAPSVPLLNQTSRT 808

Query: 889  SSSIPVNVDGYRPSGGNTRVPV----QPVTRPDGLVDSSVAGQDWRPTGRMRGSLSGQAY 1056
              S PV+ DG R S G  R  +    Q V R DGL+DS+ + Q+WRP+GRMRGSL+G+AY
Sbjct: 809  GQSRPVSADGLRASTGEQRGNMGGMSQAVPRADGLLDST-SEQNWRPSGRMRGSLAGRAY 867

Query: 1057 SAALNQYISLPTQPVQPAARXXXXXXXXXXXXXXXXHGLMENGRNSHSAQAS 1212
            S AL+Q++  PTQ  Q  AR                  L+ N RN+ S Q S
Sbjct: 868  SDALSQFMIQPTQTAQ-TARPPPNSTPPASNVLPQLQALIANNRNARSPQTS 918


>ref|XP_023911114.1| E4 SUMO-protein ligase PIAL2-like isoform X1 [Quercus suber]
          Length = 948

 Score =  216 bits (551), Expect = 2e-57
 Identities = 161/412 (39%), Positives = 210/412 (50%), Gaps = 19/412 (4%)
 Frame = +1

Query: 34   GGVSESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAPGSTPLQQFGTC 213
            GG+SE  PAN M SP+LTDA+SPAL+ EPE    T   TS++QSQ S+P +  LQQ    
Sbjct: 540  GGISEPIPANLMQSPLLTDAVSPALSLEPEGHGNTNFTTSLIQSQ-SSPNNFQLQQSQYA 598

Query: 214  NMINEYGRSPSISI---NVNRTPVAVQALPAQTPTSTMQQRSRMASQPS----------P 354
              INEYGR P++     +VNRTP+AVQALPAQ+ T   Q+R R +  P+          P
Sbjct: 599  QSINEYGRLPNLPFIHRHVNRTPIAVQALPAQSQTPNPQERPRSSLTPNGSLISPQVALP 658

Query: 355  TPLMTDGSSTAFSTVERQQQYRSHLNXXXXXXXXXXXXXXXHIGNWGPH-GHLFSSGRPS 531
                 D  +T  S +ERQQ +R+ LN                +     H     +S R  
Sbjct: 659  MAPTADVFNTICSDMERQQHFRAQLNPLQVSDIPSSLLQRPSVIQSRDHQDRSHASSRSH 718

Query: 532  QQINGLPSQQIHTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRMPHLRTQSPSPNR 711
                 +P    HT  A S+  S Y+ +  +L TE QN H  QA + RMP    QS SP  
Sbjct: 719  VSSPSVPPGLGHT--AQSRLQSHYKATSGLL-TEFQNSHLQQAFNPRMPQSVGQSSSPIP 775

Query: 712  SSSHLPQSQTNQGGLQNRVS-LTPVGGYQKDQFLVAAQRASQMARMHSPSPSVQVQTPRT 888
             SSH  ++Q  QGG Q  +S      G Q  ++LV   R        +PS  +  QT RT
Sbjct: 776  PSSHPSRTQVQQGGGQVGISQRAGTTGSQPARYLVNQNRYHPQNSRQAPSVPLLNQTSRT 835

Query: 889  SSSIPVNVDGYRPSGGNTRVPV----QPVTRPDGLVDSSVAGQDWRPTGRMRGSLSGQAY 1056
              S PV+ DG R S G  R  +    Q V R DGL+DS+ + Q+WRP+GRMRGSL+G+AY
Sbjct: 836  GQSRPVSADGLRASTGEQRGNMGGMSQAVPRADGLLDST-SEQNWRPSGRMRGSLAGRAY 894

Query: 1057 SAALNQYISLPTQPVQPAARXXXXXXXXXXXXXXXXHGLMENGRNSHSAQAS 1212
            S AL+Q++  PTQ  Q  AR                  L+ N RN+ S Q S
Sbjct: 895  SDALSQFMIQPTQTAQ-TARPPPNSTPPASNVLPQLQALIANNRNARSPQTS 945


>ref|XP_017192140.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like, partial [Malus
            domestica]
          Length = 677

 Score =  200 bits (509), Expect = 7e-53
 Identities = 148/382 (38%), Positives = 197/382 (51%), Gaps = 21/382 (5%)
 Frame = +1

Query: 34   GGVSESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAPGSTPLQ-QFGT 210
            GG+S S PAN++  PVLTDA+SPAL+R  E+   T L  S + +QFS+      Q QF +
Sbjct: 282  GGISHSGPANYLHLPVLTDAVSPALDRGTESRVNTDLVASAMHTQFSSNNLQLQQPQFAS 341

Query: 211  CN--MINEYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSRMASQPSPTPLMT----- 369
             N  + NEYGR  +  +   RTP AVQALPAQTP   +QQRSR +    P+  ++     
Sbjct: 342  SNATVSNEYGRFANTVLP--RTPTAVQALPAQTPG--LQQRSRTSFNTPPSASLSSQVGQ 397

Query: 370  ------DGSSTAFSTVERQQQY---RSHLNXXXXXXXXXXXXXXXHIGNWGPHGHLFSSG 522
                  +G +   S VERQQ +   R +L                   NW  HG      
Sbjct: 398  SITPTANGVTAVCSDVERQQHFSRPRMNLPQVSIIAPSSMQPPSQTTQNWDRHG------ 451

Query: 523  RPSQQINGLPSQQIHTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRMPHLRTQSPS 702
            + +QQ+ GLP        A SQ  S  R S  ++  E QN H  QA + R P    Q  S
Sbjct: 452  QSAQQVVGLP--------APSQLQSVNRTSLGLM--EFQNAHLQQAFNPRTPQTVGQLSS 501

Query: 703  PNRSSSHLPQSQTNQGGLQNRVSLTPVGGYQKDQFLVAAQRASQMARMHSPSPSVQVQTP 882
             NRSSSH+ ++   QG ++     T      + +F +  Q+   M    SPS  VQ Q+P
Sbjct: 502  ANRSSSHVSRAHIQQGSVRVETGQTSSSLDNQQRFAIGQQQRVAMMARQSPSTPVQNQSP 561

Query: 883  RTSSSIPVNVDGYRPSG----GNTRVPVQPVTRPDGLVDSSVAGQDWRPTGRMRGSLSGQ 1050
            R    +P N +G+R +      N    VQ V+  DG  D S +G++WRPTGRMRGSLSG+
Sbjct: 562  RNRPILPANAEGFRTAALEQSRNVVETVQAVSGADGSPDLS-SGENWRPTGRMRGSLSGR 620

Query: 1051 AYSAALNQYISLPTQPVQPAAR 1116
            AYSAALNQ+I  PTQP   A+R
Sbjct: 621  AYSAALNQFIIQPTQPTPAASR 642


>ref|XP_022737523.1| E4 SUMO-protein ligase PIAL2-like isoform X3 [Durio zibethinus]
          Length = 893

 Score =  200 bits (509), Expect = 6e-52
 Identities = 166/443 (37%), Positives = 226/443 (51%), Gaps = 24/443 (5%)
 Frame = +1

Query: 1    GASGASFLAQSGGVSESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAP 180
            G S A   AQ GG+SES P+N  +SPV +DAISPA NR  EA     L    +Q+Q SA 
Sbjct: 481  GISTARTDAQVGGISESTPSNFTVSPVFSDAISPAPNRT-EARGNANLTALGIQNQVSAA 539

Query: 181  GSTPLQQFGTCNMI--NEYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSR-----MA 339
             +  LQQ    N +  +EYG    I  ++NRTP+AVQALPA + T T+QQRSR     ++
Sbjct: 540  SNLQLQQSQLINSMPTHEYGTLQHIPRHLNRTPIAVQALPALSQTPTLQQRSRNSLNTLS 599

Query: 340  SQPSPTPLMTDGSS--TAFSTVERQQQY-RSHLNXXXXXXXXXXXXXXXHIGNWGPHGHL 510
            +  SP P   + S   +  S +ERQ Q+ RS  N                  +W     L
Sbjct: 600  TNGSPLPPQANMSMAPSVSSDMERQPQFSRSPANPHQ---------------SWNQQERL 644

Query: 511  FSSGRPSQQINGLPSQQIHTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRMPHLRT 690
            F+ G+  QQ+    S Q+         P  YR S  +L  E QN H  QA++ R+   R+
Sbjct: 645  FAPGQSVQQVGVAASSQL---------PGSYRASSGLLG-EYQNPHLQQALNPRVSQPRS 694

Query: 691  QSPSPNRSSSHLPQSQTNQGGLQNRVSLTPVGGYQKD--QFLVAAQRASQMARMHSPSPS 864
             SP   RS S L ++QT QG  Q  +  T  G    +  +F+ AAQRA+QMAR   P  +
Sbjct: 695  PSPGLIRSPSPLLRTQTQQGAAQVGLGYT-AGNVNSNPTRFMAAAQRATQMARQ-PPMVA 752

Query: 865  VQVQTPRTSSSIPVNVDGYRPSGGNTRVPVQPVTRPDGLVDSS---VAGQDWRPTGRMRG 1035
            VQ Q+ R ++S   NVDG R S    R+ +  +T      D+S   V+ Q+WRPTGRMRG
Sbjct: 753  VQTQSSR-AASYSGNVDGSRASAVEQRLNMGGLTPAASRADTSADLVSEQNWRPTGRMRG 811

Query: 1036 SLSGQAYSAALNQYISLPTQPVQPAARXXXXXXXXXXXXXXXXHGLMENGRN------SH 1197
            SL+G+AYS AL+Q +  PTQ  Q  A                   L+ N RN       +
Sbjct: 812  SLTGRAYSEALSQLMIQPTQSAQ--AARPQANLTSSPSVPPHLQALLANSRNPAVPQMQN 869

Query: 1198 SAQASTAGISGA---LQEKSSGM 1257
            +A   TAG++G+   L ++S GM
Sbjct: 870  NAITETAGMNGSSVVLPDRSGGM 892


>ref|XP_008348548.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like [Malus domestica]
          Length = 896

 Score =  200 bits (509), Expect = 6e-52
 Identities = 148/382 (38%), Positives = 197/382 (51%), Gaps = 21/382 (5%)
 Frame = +1

Query: 34   GGVSESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAPGSTPLQ-QFGT 210
            GG+S S PAN++  PVLTDA+SPAL+R  E+   T L  S + +QFS+      Q QF +
Sbjct: 501  GGISHSGPANYLHLPVLTDAVSPALDRGTESRVNTDLVASAMHTQFSSNNLQLQQPQFAS 560

Query: 211  CN--MINEYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSRMASQPSPTPLMT----- 369
             N  + NEYGR  +  +   RTP AVQALPAQTP   +QQRSR +    P+  ++     
Sbjct: 561  SNATVSNEYGRFANTVLP--RTPTAVQALPAQTPG--LQQRSRTSFNTPPSASLSSQVGQ 616

Query: 370  ------DGSSTAFSTVERQQQY---RSHLNXXXXXXXXXXXXXXXHIGNWGPHGHLFSSG 522
                  +G +   S VERQQ +   R +L                   NW  HG      
Sbjct: 617  SITPTANGVTAVCSDVERQQHFSRPRMNLPQVSIIAPSSMQPPSQTTQNWDRHG------ 670

Query: 523  RPSQQINGLPSQQIHTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRMPHLRTQSPS 702
            + +QQ+ GLP        A SQ  S  R S  ++  E QN H  QA + R P    Q  S
Sbjct: 671  QSAQQVVGLP--------APSQLQSVNRTSLGLM--EFQNAHLQQAFNPRTPQTVGQLSS 720

Query: 703  PNRSSSHLPQSQTNQGGLQNRVSLTPVGGYQKDQFLVAAQRASQMARMHSPSPSVQVQTP 882
             NRSSSH+ ++   QG ++     T      + +F +  Q+   M    SPS  VQ Q+P
Sbjct: 721  ANRSSSHVSRAHIQQGSVRVETGQTSSSLDNQQRFAIGQQQRVAMMARQSPSTPVQNQSP 780

Query: 883  RTSSSIPVNVDGYRPSG----GNTRVPVQPVTRPDGLVDSSVAGQDWRPTGRMRGSLSGQ 1050
            R    +P N +G+R +      N    VQ V+  DG  D S +G++WRPTGRMRGSLSG+
Sbjct: 781  RNRPILPANAEGFRTAALEQSRNVVETVQAVSGADGSPDLS-SGENWRPTGRMRGSLSGR 839

Query: 1051 AYSAALNQYISLPTQPVQPAAR 1116
            AYSAALNQ+I  PTQP   A+R
Sbjct: 840  AYSAALNQFIIQPTQPTPAASR 861


>ref|XP_022737522.1| E4 SUMO-protein ligase PIAL2-like isoform X2 [Durio zibethinus]
          Length = 914

 Score =  200 bits (509), Expect = 7e-52
 Identities = 166/443 (37%), Positives = 226/443 (51%), Gaps = 24/443 (5%)
 Frame = +1

Query: 1    GASGASFLAQSGGVSESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAP 180
            G S A   AQ GG+SES P+N  +SPV +DAISPA NR  EA     L    +Q+Q SA 
Sbjct: 502  GISTARTDAQVGGISESTPSNFTVSPVFSDAISPAPNRT-EARGNANLTALGIQNQVSAA 560

Query: 181  GSTPLQQFGTCNMI--NEYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSR-----MA 339
             +  LQQ    N +  +EYG    I  ++NRTP+AVQALPA + T T+QQRSR     ++
Sbjct: 561  SNLQLQQSQLINSMPTHEYGTLQHIPRHLNRTPIAVQALPALSQTPTLQQRSRNSLNTLS 620

Query: 340  SQPSPTPLMTDGSS--TAFSTVERQQQY-RSHLNXXXXXXXXXXXXXXXHIGNWGPHGHL 510
            +  SP P   + S   +  S +ERQ Q+ RS  N                  +W     L
Sbjct: 621  TNGSPLPPQANMSMAPSVSSDMERQPQFSRSPANPHQ---------------SWNQQERL 665

Query: 511  FSSGRPSQQINGLPSQQIHTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRMPHLRT 690
            F+ G+  QQ+    S Q+         P  YR S  +L  E QN H  QA++ R+   R+
Sbjct: 666  FAPGQSVQQVGVAASSQL---------PGSYRASSGLLG-EYQNPHLQQALNPRVSQPRS 715

Query: 691  QSPSPNRSSSHLPQSQTNQGGLQNRVSLTPVGGYQKD--QFLVAAQRASQMARMHSPSPS 864
             SP   RS S L ++QT QG  Q  +  T  G    +  +F+ AAQRA+QMAR   P  +
Sbjct: 716  PSPGLIRSPSPLLRTQTQQGAAQVGLGYT-AGNVNSNPTRFMAAAQRATQMARQ-PPMVA 773

Query: 865  VQVQTPRTSSSIPVNVDGYRPSGGNTRVPVQPVTRPDGLVDSS---VAGQDWRPTGRMRG 1035
            VQ Q+ R ++S   NVDG R S    R+ +  +T      D+S   V+ Q+WRPTGRMRG
Sbjct: 774  VQTQSSR-AASYSGNVDGSRASAVEQRLNMGGLTPAASRADTSADLVSEQNWRPTGRMRG 832

Query: 1036 SLSGQAYSAALNQYISLPTQPVQPAARXXXXXXXXXXXXXXXXHGLMENGRN------SH 1197
            SL+G+AYS AL+Q +  PTQ  Q  A                   L+ N RN       +
Sbjct: 833  SLTGRAYSEALSQLMIQPTQSAQ--AARPQANLTSSPSVPPHLQALLANSRNPAVPQMQN 890

Query: 1198 SAQASTAGISGA---LQEKSSGM 1257
            +A   TAG++G+   L ++S GM
Sbjct: 891  NAITETAGMNGSSVVLPDRSGGM 913


>ref|XP_022737521.1| E4 SUMO-protein ligase PIAL2-like isoform X1 [Durio zibethinus]
          Length = 915

 Score =  200 bits (509), Expect = 7e-52
 Identities = 166/443 (37%), Positives = 226/443 (51%), Gaps = 24/443 (5%)
 Frame = +1

Query: 1    GASGASFLAQSGGVSESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAP 180
            G S A   AQ GG+SES P+N  +SPV +DAISPA NR  EA     L    +Q+Q SA 
Sbjct: 503  GISTARTDAQVGGISESTPSNFTVSPVFSDAISPAPNRT-EARGNANLTALGIQNQVSAA 561

Query: 181  GSTPLQQFGTCNMI--NEYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSR-----MA 339
             +  LQQ    N +  +EYG    I  ++NRTP+AVQALPA + T T+QQRSR     ++
Sbjct: 562  SNLQLQQSQLINSMPTHEYGTLQHIPRHLNRTPIAVQALPALSQTPTLQQRSRNSLNTLS 621

Query: 340  SQPSPTPLMTDGSS--TAFSTVERQQQY-RSHLNXXXXXXXXXXXXXXXHIGNWGPHGHL 510
            +  SP P   + S   +  S +ERQ Q+ RS  N                  +W     L
Sbjct: 622  TNGSPLPPQANMSMAPSVSSDMERQPQFSRSPANPHQ---------------SWNQQERL 666

Query: 511  FSSGRPSQQINGLPSQQIHTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRMPHLRT 690
            F+ G+  QQ+    S Q+         P  YR S  +L  E QN H  QA++ R+   R+
Sbjct: 667  FAPGQSVQQVGVAASSQL---------PGSYRASSGLLG-EYQNPHLQQALNPRVSQPRS 716

Query: 691  QSPSPNRSSSHLPQSQTNQGGLQNRVSLTPVGGYQKD--QFLVAAQRASQMARMHSPSPS 864
             SP   RS S L ++QT QG  Q  +  T  G    +  +F+ AAQRA+QMAR   P  +
Sbjct: 717  PSPGLIRSPSPLLRTQTQQGAAQVGLGYT-AGNVNSNPTRFMAAAQRATQMARQ-PPMVA 774

Query: 865  VQVQTPRTSSSIPVNVDGYRPSGGNTRVPVQPVTRPDGLVDSS---VAGQDWRPTGRMRG 1035
            VQ Q+ R ++S   NVDG R S    R+ +  +T      D+S   V+ Q+WRPTGRMRG
Sbjct: 775  VQTQSSR-AASYSGNVDGSRASAVEQRLNMGGLTPAASRADTSADLVSEQNWRPTGRMRG 833

Query: 1036 SLSGQAYSAALNQYISLPTQPVQPAARXXXXXXXXXXXXXXXXHGLMENGRN------SH 1197
            SL+G+AYS AL+Q +  PTQ  Q  A                   L+ N RN       +
Sbjct: 834  SLTGRAYSEALSQLMIQPTQSAQ--AARPQANLTSSPSVPPHLQALLANSRNPAVPQMQN 891

Query: 1198 SAQASTAGISGA---LQEKSSGM 1257
            +A   TAG++G+   L ++S GM
Sbjct: 892  NAITETAGMNGSSVVLPDRSGGM 914


>ref|XP_021820820.1| E4 SUMO-protein ligase PIAL2 isoform X2 [Prunus avium]
          Length = 868

 Score =  197 bits (502), Expect = 5e-51
 Identities = 158/388 (40%), Positives = 199/388 (51%), Gaps = 19/388 (4%)
 Frame = +1

Query: 4    ASGASFLAQSGGV-SESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAP 180
            ASG     Q GGV   S PAN + SPVLTDA+SPAL+R  E+  TT L  S +  QFS+P
Sbjct: 469  ASGIRSDTQMGGVIPHSGPAN-LQSPVLTDAVSPALDRGTESHLTTDLVASAIH-QFSSP 526

Query: 181  GSTPLQQ--FGTCNMINEYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSRMA---SQ 345
             +  LQQ  F +    NEYGR  S  + + RTP AVQALPAQ+    +QQR R +   S 
Sbjct: 527  NNFQLQQLQFASSAANNEYGRFASHRV-LPRTPTAVQALPAQSQGPGLQQRPRTSWNSST 585

Query: 346  PSPTPLMT----------DGSSTAFSTVERQQQY-RSHLNXXXXXXXXXXXXXXXHIG-N 489
            PS  PL +          +G +   S +ERQQ + R  +N                   N
Sbjct: 586  PSSAPLSSQVGQSITPTANGVNAVCSDLERQQHFSRPRMNPLVSNIASSSQQHPSQTTQN 645

Query: 490  WGPHGHLFSSGRPSQQINGLPSQQIHTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQ 669
            W      F  G+  QQ+ GLP          SQ  S  R SP ++  +  N H  QA + 
Sbjct: 646  WDRQDQSFIHGQSVQQVVGLPVP--------SQLQSANRASPGLM--DFNNAHLQQAFNN 695

Query: 670  -RMPHLRTQSPSPNRSSSHLPQSQTNQGGLQNRVSLTPVGGYQKDQFLVAAQRASQMARM 846
             R P    QS S  RSSSHL ++    G  Q     T      + +F+  A RA+ +AR 
Sbjct: 696  ARTPQTMGQSSSSIRSSSHLSRAHIQPGIAQVGTGQTSSSLNNQQRFMPGAHRAAMIARQ 755

Query: 847  HSPSPSVQVQTPRTSSSIPVNVDGYRPSGGNTRVPVQPVTRPDGLVDSSVAGQDWRPTGR 1026
             SPS  VQ QTPRT  S+PVN+ G           ++ V+  DG VD S + Q+WRPTGR
Sbjct: 756  -SPSMPVQNQTPRTRPSLPVNIGG----------AMEAVSGADGSVDLS-SEQNWRPTGR 803

Query: 1027 MRGSLSGQAYSAALNQYISLPTQPVQPA 1110
            MRGSLSG+AYSAA NQ+I  PTQP Q A
Sbjct: 804  MRGSLSGRAYSAAFNQFIIAPTQPTQAA 831


>ref|XP_021820818.1| E4 SUMO-protein ligase PIAL2 isoform X1 [Prunus avium]
          Length = 892

 Score =  197 bits (502), Expect = 5e-51
 Identities = 158/388 (40%), Positives = 199/388 (51%), Gaps = 19/388 (4%)
 Frame = +1

Query: 4    ASGASFLAQSGGV-SESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAP 180
            ASG     Q GGV   S PAN + SPVLTDA+SPAL+R  E+  TT L  S +  QFS+P
Sbjct: 493  ASGIRSDTQMGGVIPHSGPAN-LQSPVLTDAVSPALDRGTESHLTTDLVASAIH-QFSSP 550

Query: 181  GSTPLQQ--FGTCNMINEYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSRMA---SQ 345
             +  LQQ  F +    NEYGR  S  + + RTP AVQALPAQ+    +QQR R +   S 
Sbjct: 551  NNFQLQQLQFASSAANNEYGRFASHRV-LPRTPTAVQALPAQSQGPGLQQRPRTSWNSST 609

Query: 346  PSPTPLMT----------DGSSTAFSTVERQQQY-RSHLNXXXXXXXXXXXXXXXHIG-N 489
            PS  PL +          +G +   S +ERQQ + R  +N                   N
Sbjct: 610  PSSAPLSSQVGQSITPTANGVNAVCSDLERQQHFSRPRMNPLVSNIASSSQQHPSQTTQN 669

Query: 490  WGPHGHLFSSGRPSQQINGLPSQQIHTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQ 669
            W      F  G+  QQ+ GLP          SQ  S  R SP ++  +  N H  QA + 
Sbjct: 670  WDRQDQSFIHGQSVQQVVGLPVP--------SQLQSANRASPGLM--DFNNAHLQQAFNN 719

Query: 670  -RMPHLRTQSPSPNRSSSHLPQSQTNQGGLQNRVSLTPVGGYQKDQFLVAAQRASQMARM 846
             R P    QS S  RSSSHL ++    G  Q     T      + +F+  A RA+ +AR 
Sbjct: 720  ARTPQTMGQSSSSIRSSSHLSRAHIQPGIAQVGTGQTSSSLNNQQRFMPGAHRAAMIARQ 779

Query: 847  HSPSPSVQVQTPRTSSSIPVNVDGYRPSGGNTRVPVQPVTRPDGLVDSSVAGQDWRPTGR 1026
             SPS  VQ QTPRT  S+PVN+ G           ++ V+  DG VD S + Q+WRPTGR
Sbjct: 780  -SPSMPVQNQTPRTRPSLPVNIGG----------AMEAVSGADGSVDLS-SEQNWRPTGR 827

Query: 1027 MRGSLSGQAYSAALNQYISLPTQPVQPA 1110
            MRGSLSG+AYSAA NQ+I  PTQP Q A
Sbjct: 828  MRGSLSGRAYSAAFNQFIIAPTQPTQAA 855


>gb|EOY24861.1| RING/U-box superfamily protein, putative [Theobroma cacao]
          Length = 919

 Score =  195 bits (495), Expect = 5e-50
 Identities = 169/448 (37%), Positives = 215/448 (47%), Gaps = 28/448 (6%)
 Frame = +1

Query: 1    GASGASFLAQSGGVSESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAP 180
            GAS A   AQ GG+SES P N  +SPV +DAISPA NR  EA     L T  +Q+QFSA 
Sbjct: 501  GASSARTDAQVGGISESTP-NFTVSPVFSDAISPAPNRA-EARGNANLTTLGIQNQFSAA 558

Query: 181  GSTPLQQFGTCNMIN--EYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSR-----MA 339
             +  LQQ    N  +  EYGR   I  ++NRTPVA+QALPA + T T QQR R     ++
Sbjct: 559  SNLQLQQSHLINSTSNHEYGRLQHIPRHINRTPVAIQALPATSQTPTQQQRPRNSLSTLS 618

Query: 340  SQPSPTPLMT-------DGSSTAFSTVERQQQY-RSHLNXXXXXXXXXXXXXXXHIGNWG 495
            S  SP P +        +G ST    VER  Q+ RS  N                  +W 
Sbjct: 619  SNGSPLPQVNLSMAPSLNGLSTVSGDVERPPQFSRSPANPHQ---------------SWN 663

Query: 496  PHGHLFSSGRPSQQINGLPSQQIHTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRM 675
                LF  G   QQ+ G+         A SQ P  YR S   L  E QNL Q Q ++ R+
Sbjct: 664  QQERLFVPGPSVQQVAGVA--------ASSQLPGSYRASSGHLG-EQQNLQQQQQLNMRL 714

Query: 676  PHLRTQSPSPNRSSSHLPQSQTNQGGLQNRVSLTPVG-GYQKDQFLVAAQRASQMARMHS 852
               R  SP   RS S L ++ T Q   Q  +  T         +F    QRA+QM R   
Sbjct: 715  SQPRGPSPGLIRSPSPLLRTPTQQVAAQVGLGHTASNVNNNPTRFGTPTQRATQMTRQ-P 773

Query: 853  PSPSVQVQTPRTSSSIPVNVDGYRPSGGNTRVPVQPVTRPDGLVDSS---VAGQDWRPTG 1023
            P   VQ QT R SSS    VDG R S G  R+ +  +       D+S    + Q+WRPTG
Sbjct: 774  PMVPVQTQTSRASSSYSGIVDGSRASAGEQRLNMVGLAPAALRADTSSDLASEQNWRPTG 833

Query: 1024 RMRGSLSGQAYSAALNQYISLPTQPVQPAARXXXXXXXXXXXXXXXXHGLMENGRNS--- 1194
            RMRGSLSG+AYSAAL+Q +  PTQ  Q  A                   L+ N RN+   
Sbjct: 834  RMRGSLSGRAYSAALSQLMIQPTQSAQ--AARPQTNLTSPPSVSPHLQALLVNSRNASVP 891

Query: 1195 ---HSAQASTAGISGA---LQEKSSGMN 1260
               ++A     G++G+   L ++SSGM+
Sbjct: 892  QMQNNAMTEIGGMNGSSNFLPDRSSGMH 919


>ref|XP_017973228.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X2 [Theobroma cacao]
          Length = 918

 Score =  194 bits (494), Expect = 7e-50
 Identities = 168/448 (37%), Positives = 215/448 (47%), Gaps = 28/448 (6%)
 Frame = +1

Query: 1    GASGASFLAQSGGVSESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAP 180
            GAS A   AQ GG+SES P N  +SPV +DAISPA NR  EA     L T  +Q+QFSA 
Sbjct: 500  GASSARTDAQVGGISESTP-NFTVSPVFSDAISPAPNRA-EARGNANLTTLGIQNQFSAA 557

Query: 181  GSTPLQQFGTCNMIN--EYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSR-----MA 339
             +  LQQ    N  +  EYGR   I  ++NRTPVA+QALPA + T T QQR R     ++
Sbjct: 558  SNLQLQQSHLINSTSNHEYGRLQHIPRHINRTPVAIQALPATSQTPTQQQRPRNSLSTLS 617

Query: 340  SQPSPTPLMT-------DGSSTAFSTVERQQQY-RSHLNXXXXXXXXXXXXXXXHIGNWG 495
            S  SP P +        +G ST    V+R  Q+ RS  N                  +W 
Sbjct: 618  SNGSPLPQVNLSMAPSLNGLSTVSGDVDRPPQFSRSPANPHQ---------------SWN 662

Query: 496  PHGHLFSSGRPSQQINGLPSQQIHTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRM 675
                LF  G   QQ+ G+         A SQ P  YR S   L  E QNL Q Q ++ R+
Sbjct: 663  QQERLFVPGPSVQQVAGVA--------ASSQLPGSYRASSGHLG-EQQNLQQQQQLNMRL 713

Query: 676  PHLRTQSPSPNRSSSHLPQSQTNQGGLQNRVSLTPVG-GYQKDQFLVAAQRASQMARMHS 852
               R  SP   RS S L ++ T Q   Q  +  T         +F    QRA+QM R   
Sbjct: 714  SQPRGPSPGLIRSPSPLLRTPTQQVAAQVGLGHTASNVNNNPTRFGTPTQRATQMTRQ-P 772

Query: 853  PSPSVQVQTPRTSSSIPVNVDGYRPSGGNTRVPVQPVTRPDGLVDSS---VAGQDWRPTG 1023
            P   VQ QT R SSS    VDG R S G  R+ +  +       D+S    + Q+WRPTG
Sbjct: 773  PMVPVQTQTSRASSSYSGIVDGSRASAGEQRLNMVGLAPAALRADTSSDLASEQNWRPTG 832

Query: 1024 RMRGSLSGQAYSAALNQYISLPTQPVQPAARXXXXXXXXXXXXXXXXHGLMENGRNS--- 1194
            RMRGSLSG+AYSAAL+Q +  PTQ  Q  A                   L+ N RN+   
Sbjct: 833  RMRGSLSGRAYSAALSQLMIQPTQSAQ--AARPQTNLTSPPSVSPHLQALLVNSRNASVP 890

Query: 1195 ---HSAQASTAGISGA---LQEKSSGMN 1260
               ++A     G++G+   L ++SSGM+
Sbjct: 891  QMQNNAMTEIGGVNGSSNFLPDRSSGMH 918


>ref|XP_007040360.2| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Theobroma cacao]
          Length = 919

 Score =  194 bits (494), Expect = 7e-50
 Identities = 168/448 (37%), Positives = 215/448 (47%), Gaps = 28/448 (6%)
 Frame = +1

Query: 1    GASGASFLAQSGGVSESAPANHMLSPVLTDAISPALNREPEALRTTALATSVVQSQFSAP 180
            GAS A   AQ GG+SES P N  +SPV +DAISPA NR  EA     L T  +Q+QFSA 
Sbjct: 501  GASSARTDAQVGGISESTP-NFTVSPVFSDAISPAPNRA-EARGNANLTTLGIQNQFSAA 558

Query: 181  GSTPLQQFGTCNMIN--EYGRSPSISINVNRTPVAVQALPAQTPTSTMQQRSR-----MA 339
             +  LQQ    N  +  EYGR   I  ++NRTPVA+QALPA + T T QQR R     ++
Sbjct: 559  SNLQLQQSHLINSTSNHEYGRLQHIPRHINRTPVAIQALPATSQTPTQQQRPRNSLSTLS 618

Query: 340  SQPSPTPLMT-------DGSSTAFSTVERQQQY-RSHLNXXXXXXXXXXXXXXXHIGNWG 495
            S  SP P +        +G ST    V+R  Q+ RS  N                  +W 
Sbjct: 619  SNGSPLPQVNLSMAPSLNGLSTVSGDVDRPPQFSRSPANPHQ---------------SWN 663

Query: 496  PHGHLFSSGRPSQQINGLPSQQIHTRPALSQHPSGYRVSPPVLATEGQNLHQHQAISQRM 675
                LF  G   QQ+ G+         A SQ P  YR S   L  E QNL Q Q ++ R+
Sbjct: 664  QQERLFVPGPSVQQVAGVA--------ASSQLPGSYRASSGHLG-EQQNLQQQQQLNMRL 714

Query: 676  PHLRTQSPSPNRSSSHLPQSQTNQGGLQNRVSLTPVG-GYQKDQFLVAAQRASQMARMHS 852
               R  SP   RS S L ++ T Q   Q  +  T         +F    QRA+QM R   
Sbjct: 715  SQPRGPSPGLIRSPSPLLRTPTQQVAAQVGLGHTASNVNNNPTRFGTPTQRATQMTRQ-P 773

Query: 853  PSPSVQVQTPRTSSSIPVNVDGYRPSGGNTRVPVQPVTRPDGLVDSS---VAGQDWRPTG 1023
            P   VQ QT R SSS    VDG R S G  R+ +  +       D+S    + Q+WRPTG
Sbjct: 774  PMVPVQTQTSRASSSYSGIVDGSRASAGEQRLNMVGLAPAALRADTSSDLASEQNWRPTG 833

Query: 1024 RMRGSLSGQAYSAALNQYISLPTQPVQPAARXXXXXXXXXXXXXXXXHGLMENGRNS--- 1194
            RMRGSLSG+AYSAAL+Q +  PTQ  Q  A                   L+ N RN+   
Sbjct: 834  RMRGSLSGRAYSAALSQLMIQPTQSAQ--AARPQTNLTSPPSVSPHLQALLVNSRNASVP 891

Query: 1195 ---HSAQASTAGISGA---LQEKSSGMN 1260
               ++A     G++G+   L ++SSGM+
Sbjct: 892  QMQNNAMTEIGGVNGSSNFLPDRSSGMH 919