BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Acanthopanax21_contig00020093
         (1205 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|KZN11953.1| hypothetical protein DCAR_004609 [Daucus carota s...   213   1e-57
ref|XP_017230627.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   213   1e-57
ref|XP_017237104.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   206   2e-55
gb|KZN07967.1| hypothetical protein DCAR_000636 [Daucus carota s...   200   2e-53
ref|XP_017237143.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   200   2e-53
gb|ONI07224.1| hypothetical protein PRUPE_5G107000 [Prunus persica]   134   2e-30
ref|XP_020419685.1| E4 SUMO-protein ligase PIAL2 [Prunus persica...   134   2e-30
ref|XP_008239090.1| PREDICTED: E4 SUMO-protein ligase PIAL2 [Pru...   129   7e-29
ref|XP_017192140.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   127   5e-28
ref|XP_008348548.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   127   6e-28
ref|XP_008375961.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   124   4e-27
ref|XP_023911115.1| E4 SUMO-protein ligase PIAL2-like isoform X2...   122   2e-26
ref|XP_023911114.1| E4 SUMO-protein ligase PIAL2-like isoform X1...   122   2e-26
ref|XP_009363385.1| PREDICTED: uncharacterized protein LOC103953...   119   7e-26
ref|XP_021820820.1| E4 SUMO-protein ligase PIAL2 isoform X2 [Pru...   120   1e-25
ref|XP_021820818.1| E4 SUMO-protein ligase PIAL2 isoform X1 [Pru...   120   1e-25
ref|XP_009343862.1| PREDICTED: E4 SUMO-protein ligase PIAL2 [Pyr...   119   2e-25
ref|XP_004300819.1| PREDICTED: uncharacterized protein LOC101293...   114   2e-23
ref|XP_019071879.1| PREDICTED: E4 SUMO-protein ligase PIAL1 isof...   114   2e-23
emb|CAN82727.1| hypothetical protein VITISV_040324 [Vitis vinifera]   114   2e-23

>gb|KZN11953.1| hypothetical protein DCAR_004609 [Daucus carota subsp. sativus]
          Length = 1030

 Score =  213 bits (543), Expect = 1e-57
 Identities = 137/279 (49%), Positives = 164/279 (58%)
 Frame = +2

Query: 2    STMQQSSRMGSQPSPTPLMMDGSSTAFSNVERQQQFWSHLNSLQGSYMSSYSLQQQIGNW 181
            S  QQ     SQ SP P+M++GS  + SNVE QQQ       LQGSYMSS +LQQQIG+W
Sbjct: 576  SVSQQRPMSTSQMSPKPVMVNGSGFS-SNVEGQQQ----PRPLQGSYMSSSTLQQQIGSW 630

Query: 182  GPHGHLFSSSRPFHQINGLPAQQIHTRPALSQHPSGYRVSPVLATESHQHQAVSQRMPHL 361
            GP GHLFS SRP        +QQ+ T  A    P GYRV       SHQ     QRMPH+
Sbjct: 631  GPQGHLFSPSRP--------SQQMSTPLA----PGGYRVPFSNGQNSHQQHFTGQRMPHI 678

Query: 362  RTQSPSLNRSSAHLPQSQTHQGGLQNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPS 541
            R+QSP L R+  HLP +QTH GG+ NR S   V   Q + F                   
Sbjct: 679  RSQSPGLARTPPHLPPTQTHHGGMPNRASSPYV--RQQEQFHSFSQRTSHLPRTPSLFNP 736

Query: 542  VQVQTPRTSSSIHVNIDGYRPSGGNTRVPVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLS 721
            VQ+QT    + +  N+D +RPS  NT   +QPV  P+G V++S  EQDWRPTGRMRGSLS
Sbjct: 737  VQLQT----TPMPRNVDAHRPSISNTGGNLQPVGTPEGTVNTST-EQDWRPTGRMRGSLS 791

Query: 722  GQAYSAALSQYISLPTQPVQPAARPLASLSTSLSAVPSP 838
            G+AYS AL+QYI  PTQPVQP  RP  +  TS S +PSP
Sbjct: 792  GRAYSEALNQYIIQPTQPVQP-IRPPTNTVTSPSGIPSP 829


>ref|XP_017230627.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like [Daucus carota subsp.
            sativus]
          Length = 1031

 Score =  213 bits (543), Expect = 1e-57
 Identities = 137/279 (49%), Positives = 164/279 (58%)
 Frame = +2

Query: 2    STMQQSSRMGSQPSPTPLMMDGSSTAFSNVERQQQFWSHLNSLQGSYMSSYSLQQQIGNW 181
            S  QQ     SQ SP P+M++GS  + SNVE QQQ       LQGSYMSS +LQQQIG+W
Sbjct: 577  SVSQQRPMSTSQMSPKPVMVNGSGFS-SNVEGQQQ----PRPLQGSYMSSSTLQQQIGSW 631

Query: 182  GPHGHLFSSSRPFHQINGLPAQQIHTRPALSQHPSGYRVSPVLATESHQHQAVSQRMPHL 361
            GP GHLFS SRP        +QQ+ T  A    P GYRV       SHQ     QRMPH+
Sbjct: 632  GPQGHLFSPSRP--------SQQMSTPLA----PGGYRVPFSNGQNSHQQHFTGQRMPHI 679

Query: 362  RTQSPSLNRSSAHLPQSQTHQGGLQNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPS 541
            R+QSP L R+  HLP +QTH GG+ NR S   V   Q + F                   
Sbjct: 680  RSQSPGLARTPPHLPPTQTHHGGMPNRASSPYV--RQQEQFHSFSQRTSHLPRTPSLFNP 737

Query: 542  VQVQTPRTSSSIHVNIDGYRPSGGNTRVPVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLS 721
            VQ+QT    + +  N+D +RPS  NT   +QPV  P+G V++S  EQDWRPTGRMRGSLS
Sbjct: 738  VQLQT----TPMPRNVDAHRPSISNTGGNLQPVGTPEGTVNTST-EQDWRPTGRMRGSLS 792

Query: 722  GQAYSAALSQYISLPTQPVQPAARPLASLSTSLSAVPSP 838
            G+AYS AL+QYI  PTQPVQP  RP  +  TS S +PSP
Sbjct: 793  GRAYSEALNQYIIQPTQPVQP-IRPPTNTVTSPSGIPSP 830


>ref|XP_017237104.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Daucus carota
            subsp. sativus]
          Length = 885

 Score =  206 bits (523), Expect = 2e-55
 Identities = 130/285 (45%), Positives = 164/285 (57%), Gaps = 2/285 (0%)
 Frame = +2

Query: 2    STMQQSSRMGSQPSPTPLMMDGSSTAFSNVERQQQFWSHLNSLQGSYMSSYSLQQQIGNW 181
            S +QQ     SQ  P PLM++ S+  FSNVER+QQ  SHL+S  G+YMS  SLQQQ+ +W
Sbjct: 577  SILQQRPINASQICPNPLMVNSSNPVFSNVERRQQPMSHLSSYPGAYMSLSSLQQQVRSW 636

Query: 182  GPHGHLFSSSRPFHQINGLPAQQIHTRPALSQHPSGYRVSPVLATESHQ--HQAVSQRMP 355
            G  G  FS S+P   I         T    S+HP GYRV  V  T+ H    Q++ QRM 
Sbjct: 637  GQQGQ-FSPSQPSQPI---------TPTVSSRHPRGYRVPTVPVTDGHNLHQQSIGQRML 686

Query: 356  HLRTQSPSLNRSSAHLPQSQTHQGGLQNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPS 535
            ++R+ SPSL RSS H P +Q+  GGL NRV+ TP  G Q                     
Sbjct: 687  NIRSPSPSLVRSSVHGPPTQSPHGGLLNRVTSTPPVGQQPGQSHRSAQQASQLAGISSQM 746

Query: 536  PSVQVQTPRTSSSIHVNIDGYRPSGGNTRVPVQPVTRPDGLVDSSVAEQDWRPTGRMRGS 715
             S  VQ P    ++  +    RP  GNT   +QP T P+ LVD S A Q+WRPTGRMRGS
Sbjct: 747  LSGLVQEPPIIRNVSADS---RPLTGNTGGTIQPAT-PEVLVDMSAAGQEWRPTGRMRGS 802

Query: 716  LSGQAYSAALSQYISLPTQPVQPAARPLASLSTSLSAVPSPPHGL 850
            LSG AYSAAL+Q+I+ P QPVQ   RPL +++TS+S +P P H L
Sbjct: 803  LSGAAYSAALNQFITQPAQPVQ-TTRPLTNVATSISGIPYPQHVL 846


>gb|KZN07967.1| hypothetical protein DCAR_000636 [Daucus carota subsp. sativus]
          Length = 840

 Score =  200 bits (509), Expect = 2e-53
 Identities = 130/285 (45%), Positives = 163/285 (57%), Gaps = 2/285 (0%)
 Frame = +2

Query: 2    STMQQSSRMGSQPSPTPLMMDGSSTAFSNVERQQQFWSHLNSLQGSYMSSYSLQQQIGNW 181
            S +QQ     SQ  P PLM++ S+  FSNVER+QQ  SHL+S  G+YMS  SLQQQ  +W
Sbjct: 534  SILQQRPINASQICPNPLMVNSSNPVFSNVERRQQPMSHLSSYPGAYMSLSSLQQQ--SW 591

Query: 182  GPHGHLFSSSRPFHQINGLPAQQIHTRPALSQHPSGYRVSPVLATESHQ--HQAVSQRMP 355
            G  G  FS S+P   I         T    S+HP GYRV  V  T+ H    Q++ QRM 
Sbjct: 592  GQQGQ-FSPSQPSQPI---------TPTVSSRHPRGYRVPTVPVTDGHNLHQQSIGQRML 641

Query: 356  HLRTQSPSLNRSSAHLPQSQTHQGGLQNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPS 535
            ++R+ SPSL RSS H P +Q+  GGL NRV+ TP  G Q                     
Sbjct: 642  NIRSPSPSLVRSSVHGPPTQSPHGGLLNRVTSTPPVGQQPGQSHRSAQQASQLAGISSQM 701

Query: 536  PSVQVQTPRTSSSIHVNIDGYRPSGGNTRVPVQPVTRPDGLVDSSVAEQDWRPTGRMRGS 715
             S  VQ P    ++  +    RP  GNT   +QP T P+ LVD S A Q+WRPTGRMRGS
Sbjct: 702  LSGLVQEPPIIRNVSADS---RPLTGNTGGTIQPAT-PEVLVDMSAAGQEWRPTGRMRGS 757

Query: 716  LSGQAYSAALSQYISLPTQPVQPAARPLASLSTSLSAVPSPPHGL 850
            LSG AYSAAL+Q+I+ P QPVQ   RPL +++TS+S +P P H L
Sbjct: 758  LSGAAYSAALNQFITQPAQPVQ-TTRPLTNVATSISGIPYPQHVL 801


>ref|XP_017237143.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X2 [Daucus carota
            subsp. sativus]
          Length = 883

 Score =  200 bits (509), Expect = 2e-53
 Identities = 130/285 (45%), Positives = 163/285 (57%), Gaps = 2/285 (0%)
 Frame = +2

Query: 2    STMQQSSRMGSQPSPTPLMMDGSSTAFSNVERQQQFWSHLNSLQGSYMSSYSLQQQIGNW 181
            S +QQ     SQ  P PLM++ S+  FSNVER+QQ  SHL+S  G+YMS  SLQQQ  +W
Sbjct: 577  SILQQRPINASQICPNPLMVNSSNPVFSNVERRQQPMSHLSSYPGAYMSLSSLQQQ--SW 634

Query: 182  GPHGHLFSSSRPFHQINGLPAQQIHTRPALSQHPSGYRVSPVLATESHQ--HQAVSQRMP 355
            G  G  FS S+P   I         T    S+HP GYRV  V  T+ H    Q++ QRM 
Sbjct: 635  GQQGQ-FSPSQPSQPI---------TPTVSSRHPRGYRVPTVPVTDGHNLHQQSIGQRML 684

Query: 356  HLRTQSPSLNRSSAHLPQSQTHQGGLQNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPS 535
            ++R+ SPSL RSS H P +Q+  GGL NRV+ TP  G Q                     
Sbjct: 685  NIRSPSPSLVRSSVHGPPTQSPHGGLLNRVTSTPPVGQQPGQSHRSAQQASQLAGISSQM 744

Query: 536  PSVQVQTPRTSSSIHVNIDGYRPSGGNTRVPVQPVTRPDGLVDSSVAEQDWRPTGRMRGS 715
             S  VQ P    ++  +    RP  GNT   +QP T P+ LVD S A Q+WRPTGRMRGS
Sbjct: 745  LSGLVQEPPIIRNVSADS---RPLTGNTGGTIQPAT-PEVLVDMSAAGQEWRPTGRMRGS 800

Query: 716  LSGQAYSAALSQYISLPTQPVQPAARPLASLSTSLSAVPSPPHGL 850
            LSG AYSAAL+Q+I+ P QPVQ   RPL +++TS+S +P P H L
Sbjct: 801  LSGAAYSAALNQFITQPAQPVQ-TTRPLTNVATSISGIPYPQHVL 844


>gb|ONI07224.1| hypothetical protein PRUPE_5G107000 [Prunus persica]
          Length = 854

 Score =  134 bits (337), Expect = 2e-30
 Identities = 112/291 (38%), Positives = 143/291 (49%), Gaps = 7/291 (2%)
 Frame = +2

Query: 2    STMQQSSRMGSQPSPTPLMMDGSSTAFSNVERQQQFWS-HLNSLQGSYMSSYSLQ---QQ 169
            S+   SS++G   +PT    +G +   S++ERQQ F    +N LQ S ++S SLQ   Q 
Sbjct: 572  SSASLSSQVGQSITPTA---NGVNAVCSDLERQQHFSRPRMNPLQVSNIASSSLQHPSQT 628

Query: 170  IGNWGPHGHLFSSSRPFHQINGLPAQQIHTRPALSQHPSGYRVSPVLAT--ESHQHQAVS 343
              NW      F        I+G   QQ+   P  SQ  S  R SP L     +H  QA +
Sbjct: 629  TQNWDRQDQSF--------IHGQSVQQVVGLPVPSQLQSANRASPGLMDFQNAHLQQAFN 680

Query: 344  Q-RMPHLRTQSPSLNRSSAHLPQSQTHQGGLQNRVSPTPVGGHQLDPFLVXXXXXXXXXX 520
              R P    QS S  RSS+HL ++   QG  Q     T    +    F            
Sbjct: 681  NARTPQTMGQSSSSIRSSSHLSRAHIQQGNAQVGTGQTSSSLNNQQRFKAGTHLAAIMAR 740

Query: 521  XXXPSPSVQVQTPRTSSSIHVNIDGYRPSGGNTRVPVQPVTRPDGLVDSSVAEQDWRPTG 700
               PS  VQ QTPRT  S+ VN+ G           +Q V+  DG VD S +EQ+WRPTG
Sbjct: 741  QS-PSMPVQNQTPRTRPSLPVNVGG----------TMQAVSGADGSVDLS-SEQNWRPTG 788

Query: 701  RMRGSLSGQAYSAALSQYISLPTQPVQPAARPLASLSTSLSAVPSPPHGLM 853
            RMRGSLSG+AYSAA  Q+I  PTQP Q AARP  +L++    VPS P  L+
Sbjct: 789  RMRGSLSGRAYSAAFHQFIIAPTQPTQ-AARPPPNLTSPPPVVPSQPETLI 838


>ref|XP_020419685.1| E4 SUMO-protein ligase PIAL2 [Prunus persica]
 gb|ONI07225.1| hypothetical protein PRUPE_5G107000 [Prunus persica]
          Length = 893

 Score =  134 bits (337), Expect = 2e-30
 Identities = 112/291 (38%), Positives = 143/291 (49%), Gaps = 7/291 (2%)
 Frame = +2

Query: 2    STMQQSSRMGSQPSPTPLMMDGSSTAFSNVERQQQFWS-HLNSLQGSYMSSYSLQ---QQ 169
            S+   SS++G   +PT    +G +   S++ERQQ F    +N LQ S ++S SLQ   Q 
Sbjct: 611  SSASLSSQVGQSITPTA---NGVNAVCSDLERQQHFSRPRMNPLQVSNIASSSLQHPSQT 667

Query: 170  IGNWGPHGHLFSSSRPFHQINGLPAQQIHTRPALSQHPSGYRVSPVLAT--ESHQHQAVS 343
              NW      F        I+G   QQ+   P  SQ  S  R SP L     +H  QA +
Sbjct: 668  TQNWDRQDQSF--------IHGQSVQQVVGLPVPSQLQSANRASPGLMDFQNAHLQQAFN 719

Query: 344  Q-RMPHLRTQSPSLNRSSAHLPQSQTHQGGLQNRVSPTPVGGHQLDPFLVXXXXXXXXXX 520
              R P    QS S  RSS+HL ++   QG  Q     T    +    F            
Sbjct: 720  NARTPQTMGQSSSSIRSSSHLSRAHIQQGNAQVGTGQTSSSLNNQQRFKAGTHLAAIMAR 779

Query: 521  XXXPSPSVQVQTPRTSSSIHVNIDGYRPSGGNTRVPVQPVTRPDGLVDSSVAEQDWRPTG 700
               PS  VQ QTPRT  S+ VN+ G           +Q V+  DG VD S +EQ+WRPTG
Sbjct: 780  QS-PSMPVQNQTPRTRPSLPVNVGG----------TMQAVSGADGSVDLS-SEQNWRPTG 827

Query: 701  RMRGSLSGQAYSAALSQYISLPTQPVQPAARPLASLSTSLSAVPSPPHGLM 853
            RMRGSLSG+AYSAA  Q+I  PTQP Q AARP  +L++    VPS P  L+
Sbjct: 828  RMRGSLSGRAYSAAFHQFIIAPTQPTQ-AARPPPNLTSPPPVVPSQPETLI 877


>ref|XP_008239090.1| PREDICTED: E4 SUMO-protein ligase PIAL2 [Prunus mume]
          Length = 844

 Score =  129 bits (325), Expect = 7e-29
 Identities = 109/291 (37%), Positives = 144/291 (49%), Gaps = 7/291 (2%)
 Frame = +2

Query: 2    STMQQSSRMGSQPSPTPLMMDGSSTAFSNVERQQQFWS-HLNSLQGSYMSSYSLQ---QQ 169
            S+   SS++G   +P+    + ++   S++ERQQ F    +N LQ S ++S SLQ   Q 
Sbjct: 562  SSASLSSQVGQSITPSA---NCANAVCSDLERQQHFSRPRMNPLQVSNIASSSLQHPSQT 618

Query: 170  IGNWGPHGHLFSSSRPFHQINGLPAQQIHTRPALSQHPSGYRVSPVLAT--ESHQHQAVS 343
              NW      F        I+G   QQ+   P  SQ  S  R SP L     +H  QA +
Sbjct: 619  TQNWDRQDQSF--------IHGQSVQQVVGLPVPSQLQSANRASPGLMDFQNAHLQQAFN 670

Query: 344  Q-RMPHLRTQSPSLNRSSAHLPQSQTHQGGLQNRVSPTPVGGHQLDPFLVXXXXXXXXXX 520
              R P    QS S  RSS+HL ++   QG  Q     T    +    F+           
Sbjct: 671  NARTPQTMGQSSSSIRSSSHLSRAHIQQGNAQVGTGQTSSSLNNQQRFMAGAHLAAMMAR 730

Query: 521  XXXPSPSVQVQTPRTSSSIHVNIDGYRPSGGNTRVPVQPVTRPDGLVDSSVAEQDWRPTG 700
               PS  VQ QT RT  S+ VN+ G           +Q V+  DG VD S +EQ+WRPTG
Sbjct: 731  QS-PSMPVQNQTQRTRPSLPVNVGG----------TMQAVSGADGSVDLS-SEQNWRPTG 778

Query: 701  RMRGSLSGQAYSAALSQYISLPTQPVQPAARPLASLSTSLSAVPSPPHGLM 853
            RMRGSLSG+AYSAA +Q+I  PTQP Q AARP  +L++    VPS P  L+
Sbjct: 779  RMRGSLSGRAYSAAFNQFIIAPTQPTQ-AARPPPNLTSPPPVVPSQPQTLI 828


>ref|XP_017192140.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like, partial [Malus
            domestica]
          Length = 677

 Score =  127 bits (318), Expect = 5e-28
 Identities = 102/286 (35%), Positives = 136/286 (47%), Gaps = 10/286 (3%)
 Frame = +2

Query: 17   SSRMGSQPSPTPLMMDGSSTAFSNVERQQQFWS-HLNSLQGSYMSSYSLQ---QQIGNWG 184
            SS++G   +PT    +G +   S+VERQQ F    +N  Q S ++  S+Q   Q   NW 
Sbjct: 392  SSQVGQSITPTA---NGVTAVCSDVERQQHFSRPRMNLPQVSIIAPSSMQPPSQTTQNWD 448

Query: 185  PHGHLFSSSRPFHQINGLPAQQIHTRPALSQHPSGYRVSPVLAT--ESHQHQAVSQRMPH 358
             HG                AQQ+   PA SQ  S  R S  L     +H  QA + R P 
Sbjct: 449  RHGQ--------------SAQQVVGLPAPSQLQSVNRTSLGLMEFQNAHLQQAFNPRTPQ 494

Query: 359  LRTQSPSLNRSSAHLPQSQTHQGGLQNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSP 538
               Q  S NRSS+H+ ++   QG ++     T         F +             PS 
Sbjct: 495  TVGQLSSANRSSSHVSRAHIQQGSVRVETGQTSSSLDNQQRFAIGQQQRVAMMARQSPST 554

Query: 539  SVQVQTPRTSSSIHVNIDGYRPSG----GNTRVPVQPVTRPDGLVDSSVAEQDWRPTGRM 706
             VQ Q+PR    +  N +G+R +      N    VQ V+  DG  D S  E +WRPTGRM
Sbjct: 555  PVQNQSPRNRPILPANAEGFRTAALEQSRNVVETVQAVSGADGSPDLSSGE-NWRPTGRM 613

Query: 707  RGSLSGQAYSAALSQYISLPTQPVQPAARPLASLSTSLSAVPSPPH 844
            RGSLSG+AYSAAL+Q+I  PTQP   A+RP ++LS       +PPH
Sbjct: 614  RGSLSGRAYSAALNQFIIQPTQPTPAASRPPSNLS-------APPH 652


>ref|XP_008348548.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like [Malus domestica]
          Length = 896

 Score =  127 bits (318), Expect = 6e-28
 Identities = 102/286 (35%), Positives = 136/286 (47%), Gaps = 10/286 (3%)
 Frame = +2

Query: 17   SSRMGSQPSPTPLMMDGSSTAFSNVERQQQFWS-HLNSLQGSYMSSYSLQ---QQIGNWG 184
            SS++G   +PT    +G +   S+VERQQ F    +N  Q S ++  S+Q   Q   NW 
Sbjct: 611  SSQVGQSITPTA---NGVTAVCSDVERQQHFSRPRMNLPQVSIIAPSSMQPPSQTTQNWD 667

Query: 185  PHGHLFSSSRPFHQINGLPAQQIHTRPALSQHPSGYRVSPVLAT--ESHQHQAVSQRMPH 358
             HG                AQQ+   PA SQ  S  R S  L     +H  QA + R P 
Sbjct: 668  RHGQ--------------SAQQVVGLPAPSQLQSVNRTSLGLMEFQNAHLQQAFNPRTPQ 713

Query: 359  LRTQSPSLNRSSAHLPQSQTHQGGLQNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSP 538
               Q  S NRSS+H+ ++   QG ++     T         F +             PS 
Sbjct: 714  TVGQLSSANRSSSHVSRAHIQQGSVRVETGQTSSSLDNQQRFAIGQQQRVAMMARQSPST 773

Query: 539  SVQVQTPRTSSSIHVNIDGYRPSG----GNTRVPVQPVTRPDGLVDSSVAEQDWRPTGRM 706
             VQ Q+PR    +  N +G+R +      N    VQ V+  DG  D S  E +WRPTGRM
Sbjct: 774  PVQNQSPRNRPILPANAEGFRTAALEQSRNVVETVQAVSGADGSPDLSSGE-NWRPTGRM 832

Query: 707  RGSLSGQAYSAALSQYISLPTQPVQPAARPLASLSTSLSAVPSPPH 844
            RGSLSG+AYSAAL+Q+I  PTQP   A+RP ++LS       +PPH
Sbjct: 833  RGSLSGRAYSAALNQFIIQPTQPTPAASRPPSNLS-------APPH 871


>ref|XP_008375961.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like [Malus domestica]
          Length = 896

 Score =  124 bits (312), Expect = 4e-27
 Identities = 100/285 (35%), Positives = 137/285 (48%), Gaps = 10/285 (3%)
 Frame = +2

Query: 20   SRMGSQPSPTPLMMDGSSTAFSNVERQQQFW-SHLNSLQGSYMSSYSLQ---QQIGNWGP 187
            S++G   +PT    +G +   S++ERQQ F  + +N  Q S ++  S+Q   Q   NW  
Sbjct: 612  SQVGQSVTPTA---NGVNAVCSDMERQQHFSRARMNPPQVSNVAPPSMQPPSQTTQNWDC 668

Query: 188  HGHLFSSSRPFHQINGLPAQQIHTRPALSQHPSGYRVSPVLAT--ESHQHQAVSQRMPHL 361
            HG                AQQ+   PA SQ  S  R S  L     +H  QA++ R P  
Sbjct: 669  HGQ--------------SAQQVVGLPAPSQMQSANRTSVGLMEFQNAHLQQALNPRTPQT 714

Query: 362  RTQSPSLNRSSAHLPQSQTHQGGLQNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPS 541
                 S N SS+HL ++Q  QG ++     T    +    F++             PS  
Sbjct: 715  VGPFSSANGSSSHLSRAQIQQGSVRVGTGQTSSSLNNQQRFMIAQQQLAAMMSRQSPSTP 774

Query: 542  VQVQTPRTSSSIHVNIDGYRPS----GGNTRVPVQPVTRPDGLVDSSVAEQDWRPTGRMR 709
             Q Q+PR    +  N +G+R +    G N    VQ V+  DG  D S  E +WRPTGRMR
Sbjct: 775  DQNQSPRNRPILAANAEGFRSAAMEQGRNIVGTVQAVSGADGSPDLSSGE-NWRPTGRMR 833

Query: 710  GSLSGQAYSAALSQYISLPTQPVQPAARPLASLSTSLSAVPSPPH 844
            GSLSG+AYSAAL+Q+I  PTQP   A RP ++LS       +PPH
Sbjct: 834  GSLSGRAYSAALNQFIIQPTQPTPAAPRPPSNLS-------APPH 871


>ref|XP_023911115.1| E4 SUMO-protein ligase PIAL2-like isoform X2 [Quercus suber]
 gb|POF11949.1| e4 sumo-protein ligase pial2 [Quercus suber]
          Length = 921

 Score =  122 bits (306), Expect = 2e-26
 Identities = 102/267 (38%), Positives = 135/267 (50%), Gaps = 10/267 (3%)
 Frame = +2

Query: 62   DGSSTAFSNVERQQQFWSHLNSLQGSYMSSYSLQQQ--IGNWGPHGHLFSSSRPFHQING 235
            D  +T  S++ERQQ F + LN LQ S + S  LQ+   I +        +SSR       
Sbjct: 637  DVFNTICSDMERQQHFRAQLNPLQVSDIPSSLLQRPSVIQSRDHQDRSHASSRSHVSSPS 696

Query: 236  LPAQQIHTRPALSQHPSGYRVSPVLATE---SHQHQAVSQRMPHLRTQSPSLNRSSAHLP 406
            +P    HT  A S+  S Y+ +  L TE   SH  QA + RMP    QS S    S+H  
Sbjct: 697  VPPGLGHT--AQSRLQSHYKATSGLLTEFQNSHLQQAFNPRMPQSVGQSSSPIPPSSHPS 754

Query: 407  QSQTHQGGLQNRVSPTP-VGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHV 583
            ++Q  QGG Q  +S      G Q   +LV             PS  +  QT RT  S  V
Sbjct: 755  RTQVQQGGGQVGISQRAGTTGSQPARYLVNQNRYHPQNSRQAPSVPLLNQTSRTGQSRPV 814

Query: 584  NIDGYRPSGGNTRVPV----QPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQ 751
            + DG R S G  R  +    Q V R DGL+DS+ +EQ+WRP+GRMRGSL+G+AYS ALSQ
Sbjct: 815  SADGLRASTGEQRGNMGGMSQAVPRADGLLDST-SEQNWRPSGRMRGSLAGRAYSDALSQ 873

Query: 752  YISLPTQPVQPAARPLASLSTSLSAVP 832
            ++  PTQ  Q A  P  S   + + +P
Sbjct: 874  FMIQPTQTAQTARPPPNSTPPASNVLP 900


>ref|XP_023911114.1| E4 SUMO-protein ligase PIAL2-like isoform X1 [Quercus suber]
          Length = 948

 Score =  122 bits (306), Expect = 2e-26
 Identities = 102/267 (38%), Positives = 135/267 (50%), Gaps = 10/267 (3%)
 Frame = +2

Query: 62   DGSSTAFSNVERQQQFWSHLNSLQGSYMSSYSLQQQ--IGNWGPHGHLFSSSRPFHQING 235
            D  +T  S++ERQQ F + LN LQ S + S  LQ+   I +        +SSR       
Sbjct: 664  DVFNTICSDMERQQHFRAQLNPLQVSDIPSSLLQRPSVIQSRDHQDRSHASSRSHVSSPS 723

Query: 236  LPAQQIHTRPALSQHPSGYRVSPVLATE---SHQHQAVSQRMPHLRTQSPSLNRSSAHLP 406
            +P    HT  A S+  S Y+ +  L TE   SH  QA + RMP    QS S    S+H  
Sbjct: 724  VPPGLGHT--AQSRLQSHYKATSGLLTEFQNSHLQQAFNPRMPQSVGQSSSPIPPSSHPS 781

Query: 407  QSQTHQGGLQNRVSPTP-VGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHV 583
            ++Q  QGG Q  +S      G Q   +LV             PS  +  QT RT  S  V
Sbjct: 782  RTQVQQGGGQVGISQRAGTTGSQPARYLVNQNRYHPQNSRQAPSVPLLNQTSRTGQSRPV 841

Query: 584  NIDGYRPSGGNTRVPV----QPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQ 751
            + DG R S G  R  +    Q V R DGL+DS+ +EQ+WRP+GRMRGSL+G+AYS ALSQ
Sbjct: 842  SADGLRASTGEQRGNMGGMSQAVPRADGLLDST-SEQNWRPSGRMRGSLAGRAYSDALSQ 900

Query: 752  YISLPTQPVQPAARPLASLSTSLSAVP 832
            ++  PTQ  Q A  P  S   + + +P
Sbjct: 901  FMIQPTQTAQTARPPPNSTPPASNVLP 927


>ref|XP_009363385.1| PREDICTED: uncharacterized protein LOC103953373 [Pyrus x
            bretschneideri]
          Length = 522

 Score =  119 bits (299), Expect = 7e-26
 Identities = 96/283 (33%), Positives = 137/283 (48%), Gaps = 8/283 (2%)
 Frame = +2

Query: 20   SRMGSQPSPTPLMMDGSSTAFSNVERQQQFW-SHLNSLQGSYMSSYSLQ---QQIGNWGP 187
            S++G   +PT    +G +   S++ERQQ F  + +N  Q S ++  S+Q   Q   NW  
Sbjct: 238  SQVGQSVTPTA---NGVNAVCSDMERQQHFSRARMNPPQVSNVAPPSMQPPSQITQNWDC 294

Query: 188  HGHLFSSSRPFHQINGLPAQQIHTRPALSQHPSGYRVSPVLATESHQHQAVSQRMPHLRT 367
            HG      +   Q+ GLPA      P+  Q  +   V  +    +H  QA++   P    
Sbjct: 295  HG------QSAQQVVGLPA------PSQMQSANQTSVGLMEFQNAHLQQALNPMTPQTVG 342

Query: 368  QSPSLNRSSAHLPQSQTHQGGLQNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPSVQ 547
               S N SS+HL ++Q  QG ++     T    +    F++             PS   Q
Sbjct: 343  PFSSANGSSSHLLRAQIQQGSVRVGTGQTSSSLNNQQRFMIAQQRRAAMMARQSPSTPDQ 402

Query: 548  VQTPRTSSSIHVNIDGYRPS----GGNTRVPVQPVTRPDGLVDSSVAEQDWRPTGRMRGS 715
             Q+PR    +  N +G+R +    G N    VQ V+  DG  D S  E +WRPTGRMRGS
Sbjct: 403  NQSPRNRPILAANAEGFRSAAMEQGRNIVGTVQAVSGADGSPDLSSGE-NWRPTGRMRGS 461

Query: 716  LSGQAYSAALSQYISLPTQPVQPAARPLASLSTSLSAVPSPPH 844
            LSG+AYSAAL+Q+I  PTQP   A RP ++LS       +PPH
Sbjct: 462  LSGRAYSAALNQFIIQPTQPTPAAPRPPSNLS-------APPH 497


>ref|XP_021820820.1| E4 SUMO-protein ligase PIAL2 isoform X2 [Prunus avium]
          Length = 868

 Score =  120 bits (301), Expect = 1e-25
 Identities = 106/290 (36%), Positives = 138/290 (47%), Gaps = 6/290 (2%)
 Frame = +2

Query: 2    STMQQSSRMGSQPSPTPLMMDGSSTAFSNVERQQQFWS-HLNSLQGSYMSSYSLQ--QQI 172
            S+   SS++G   +PT    +G +   S++ERQQ F    +N L  +  SS      Q  
Sbjct: 587  SSAPLSSQVGQSITPTA---NGVNAVCSDLERQQHFSRPRMNPLVSNIASSSQQHPSQTT 643

Query: 173  GNWGPHGHLFSSSRPFHQINGLPAQQIHTRPALSQHPSGYRVSPVLA--TESHQHQAVSQ 346
             NW      F        I+G   QQ+   P  SQ  S  R SP L     +H  QA + 
Sbjct: 644  QNWDRQDQSF--------IHGQSVQQVVGLPVPSQLQSANRASPGLMDFNNAHLQQAFNN 695

Query: 347  -RMPHLRTQSPSLNRSSAHLPQSQTHQGGLQNRVSPTPVGGHQLDPFLVXXXXXXXXXXX 523
             R P    QS S  RSS+HL ++    G  Q     T    +    F+            
Sbjct: 696  ARTPQTMGQSSSSIRSSSHLSRAHIQPGIAQVGTGQTSSSLNNQQRFM-PGAHRAAMIAR 754

Query: 524  XXPSPSVQVQTPRTSSSIHVNIDGYRPSGGNTRVPVQPVTRPDGLVDSSVAEQDWRPTGR 703
              PS  VQ QTPRT  S+ VNI G           ++ V+  DG VD S +EQ+WRPTGR
Sbjct: 755  QSPSMPVQNQTPRTRPSLPVNIGG----------AMEAVSGADGSVDLS-SEQNWRPTGR 803

Query: 704  MRGSLSGQAYSAALSQYISLPTQPVQPAARPLASLSTSLSAVPSPPHGLM 853
            MRGSLSG+AYSAA +Q+I  PTQP Q AARP  +L++    V S P  L+
Sbjct: 804  MRGSLSGRAYSAAFNQFIIAPTQPTQ-AARPPPNLTSPPPVVLSQPQTLI 852


>ref|XP_021820818.1| E4 SUMO-protein ligase PIAL2 isoform X1 [Prunus avium]
          Length = 892

 Score =  120 bits (301), Expect = 1e-25
 Identities = 106/290 (36%), Positives = 138/290 (47%), Gaps = 6/290 (2%)
 Frame = +2

Query: 2    STMQQSSRMGSQPSPTPLMMDGSSTAFSNVERQQQFWS-HLNSLQGSYMSSYSLQ--QQI 172
            S+   SS++G   +PT    +G +   S++ERQQ F    +N L  +  SS      Q  
Sbjct: 611  SSAPLSSQVGQSITPTA---NGVNAVCSDLERQQHFSRPRMNPLVSNIASSSQQHPSQTT 667

Query: 173  GNWGPHGHLFSSSRPFHQINGLPAQQIHTRPALSQHPSGYRVSPVLA--TESHQHQAVSQ 346
             NW      F        I+G   QQ+   P  SQ  S  R SP L     +H  QA + 
Sbjct: 668  QNWDRQDQSF--------IHGQSVQQVVGLPVPSQLQSANRASPGLMDFNNAHLQQAFNN 719

Query: 347  -RMPHLRTQSPSLNRSSAHLPQSQTHQGGLQNRVSPTPVGGHQLDPFLVXXXXXXXXXXX 523
             R P    QS S  RSS+HL ++    G  Q     T    +    F+            
Sbjct: 720  ARTPQTMGQSSSSIRSSSHLSRAHIQPGIAQVGTGQTSSSLNNQQRFM-PGAHRAAMIAR 778

Query: 524  XXPSPSVQVQTPRTSSSIHVNIDGYRPSGGNTRVPVQPVTRPDGLVDSSVAEQDWRPTGR 703
              PS  VQ QTPRT  S+ VNI G           ++ V+  DG VD S +EQ+WRPTGR
Sbjct: 779  QSPSMPVQNQTPRTRPSLPVNIGG----------AMEAVSGADGSVDLS-SEQNWRPTGR 827

Query: 704  MRGSLSGQAYSAALSQYISLPTQPVQPAARPLASLSTSLSAVPSPPHGLM 853
            MRGSLSG+AYSAA +Q+I  PTQP Q AARP  +L++    V S P  L+
Sbjct: 828  MRGSLSGRAYSAAFNQFIIAPTQPTQ-AARPPPNLTSPPPVVLSQPQTLI 876


>ref|XP_009343862.1| PREDICTED: E4 SUMO-protein ligase PIAL2 [Pyrus x bretschneideri]
          Length = 899

 Score =  119 bits (299), Expect = 2e-25
 Identities = 96/283 (33%), Positives = 137/283 (48%), Gaps = 8/283 (2%)
 Frame = +2

Query: 20   SRMGSQPSPTPLMMDGSSTAFSNVERQQQFW-SHLNSLQGSYMSSYSLQ---QQIGNWGP 187
            S++G   +PT    +G +   S++ERQQ F  + +N  Q S ++  S+Q   Q   NW  
Sbjct: 615  SQVGQSVTPTA---NGVNAVCSDMERQQHFSRARMNPPQVSNVAPPSMQPPSQITQNWDC 671

Query: 188  HGHLFSSSRPFHQINGLPAQQIHTRPALSQHPSGYRVSPVLATESHQHQAVSQRMPHLRT 367
            HG      +   Q+ GLPA      P+  Q  +   V  +    +H  QA++   P    
Sbjct: 672  HG------QSAQQVVGLPA------PSQMQSANQTSVGLMEFQNAHLQQALNPMTPQTVG 719

Query: 368  QSPSLNRSSAHLPQSQTHQGGLQNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPSVQ 547
               S N SS+HL ++Q  QG ++     T    +    F++             PS   Q
Sbjct: 720  PFSSANGSSSHLLRAQIQQGSVRVGTGQTSSSLNNQQRFMIAQQRRAAMMARQSPSTPDQ 779

Query: 548  VQTPRTSSSIHVNIDGYRPS----GGNTRVPVQPVTRPDGLVDSSVAEQDWRPTGRMRGS 715
             Q+PR    +  N +G+R +    G N    VQ V+  DG  D S  E +WRPTGRMRGS
Sbjct: 780  NQSPRNRPILAANAEGFRSAAMEQGRNIVGTVQAVSGADGSPDLSSGE-NWRPTGRMRGS 838

Query: 716  LSGQAYSAALSQYISLPTQPVQPAARPLASLSTSLSAVPSPPH 844
            LSG+AYSAAL+Q+I  PTQP   A RP ++LS       +PPH
Sbjct: 839  LSGRAYSAALNQFIIQPTQPTPAAPRPPSNLS-------APPH 874


>ref|XP_004300819.1| PREDICTED: uncharacterized protein LOC101293006 [Fragaria vesca
            subsp. vesca]
          Length = 884

 Score =  114 bits (284), Expect = 2e-23
 Identities = 98/261 (37%), Positives = 130/261 (49%), Gaps = 7/261 (2%)
 Frame = +2

Query: 83   SNVERQQQFWS-HLNSLQGSYMSSYSLQQQIGNWGPHGHLFSSSRPFHQINGLPAQQIHT 259
            S++ERQQ F    +N +Q S ++S SLQ        HG   + +      +    Q +  
Sbjct: 613  SDLERQQYFARPQMNPVQVSGIASSSLQ--------HGSRTTQNCAPQDRSFTHGQSVVG 664

Query: 260  RPALSQHPSGYRVSPVLA--TESHQHQAVSQRMPHLRTQSPSLNRSSAHLPQSQTHQGGL 433
                SQ  S  RVS  L   T +H  +  + R P   +QSP +     H  +S   QG  
Sbjct: 665  HQVPSQLQSASRVSSGLQGFTNAHLDKTFNSRTPPAMSQSPRV-----HTLRSHVRQGSA 719

Query: 434  QNRVSPTPVGGHQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSIHVNIDGYRPSGG 613
            Q  ++  P   +    F V             P P+ Q QTPRT  S+ VN  G+R S G
Sbjct: 720  QVGINQAPSSLNNQQSFTVAAQRAAMARQSS-PMPA-QNQTPRTRPSLSVNAGGFRGSAG 777

Query: 614  NTRV----PVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAALSQYISLPTQPVQ 781
            + R     PVQ V+  D LVDS  +EQ+WRPTGRMRGSLSGQAY+AAL+++I  PT P +
Sbjct: 778  DRRGNIGGPVQAVSGADELVDSP-SEQNWRPTGRMRGSLSGQAYTAALNKFIIRPTPPTE 836

Query: 782  PAARPLASLSTSLSAVPSPPH 844
              ARP AS  T  S +  PPH
Sbjct: 837  -KARP-ASHLTPPSHLTRPPH 855


>ref|XP_019071879.1| PREDICTED: E4 SUMO-protein ligase PIAL1 isoform X2 [Vitis vinifera]
          Length = 898

 Score =  114 bits (284), Expect = 2e-23
 Identities = 98/275 (35%), Positives = 132/275 (48%), Gaps = 11/275 (4%)
 Frame = +2

Query: 53   LMMDGSSTAFSNVERQQQFW-SHLNSLQGSYMSSYSLQQQIGNWGPHGHLFSSSRPFHQI 229
            ++ +G +T  S++ER QQF  S  N +Q S +S+ +LQ                   H +
Sbjct: 612  MVPNGPNTVGSDMERPQQFSRSIFNPVQISDISASALQH------------------HSM 653

Query: 230  NGLPAQQIHTRPALSQHPS--GYRVSPVLATESHQHQAVSQRMPHLRTQSPSLNRSSAHL 403
            +    QQ+   P  SQ P    YR S  L TE    Q   Q+ P  RT S  L  S+ H 
Sbjct: 654  SQNWNQQVAGHPTTSQRPGPGAYRTSSGLPTEPQTLQ--QQQSPQARTHSNLLRSSAHHH 711

Query: 404  PQSQTHQGGLQNRVSPTPVGG--HQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSI 577
             +SQ  QGG Q R +     G      P +              P P VQ QT RT S+ 
Sbjct: 712  SRSQVQQGGAQGRATHAVGTGISQNAQPMVAAQRAAQMTRM---PLP-VQNQTSRTGSAF 767

Query: 578  HVNIDGYRPSGG----NTRVPVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAAL 745
             VN +G R + G    N    VQ V+RP+ LVD + +EQ+WRPTG MRGSL G+AY++AL
Sbjct: 768  PVNANGGRSTAGEQRGNIEGMVQAVSRPESLVDLA-SEQNWRPTGLMRGSLVGRAYNSAL 826

Query: 746  SQYISLPTQPVQPAARPLASLSTSLSAVPS--PPH 844
            +Q +  PTQP Q    P     T +++ P   PPH
Sbjct: 827  NQLVIQPTQPTQSTRPP-----TPITSPPPGFPPH 856


>emb|CAN82727.1| hypothetical protein VITISV_040324 [Vitis vinifera]
          Length = 901

 Score =  114 bits (284), Expect = 2e-23
 Identities = 98/275 (35%), Positives = 132/275 (48%), Gaps = 11/275 (4%)
 Frame = +2

Query: 53   LMMDGSSTAFSNVERQQQFW-SHLNSLQGSYMSSYSLQQQIGNWGPHGHLFSSSRPFHQI 229
            ++ +G +T  S++ER QQF  S  N +Q S +S+ +LQ                   H +
Sbjct: 615  MVPNGPNTVGSDMERPQQFSRSIFNPVQISDISASALQH------------------HSM 656

Query: 230  NGLPAQQIHTRPALSQHPS--GYRVSPVLATESHQHQAVSQRMPHLRTQSPSLNRSSAHL 403
            +    QQ+   P  SQ P    YR S  L TE    Q   Q+ P  RT S  L  S+ H 
Sbjct: 657  SQNWNQQVAGHPTTSQRPGPGAYRTSSGLPTEPQTLQ--QQQSPQARTHSNLLRSSAHHH 714

Query: 404  PQSQTHQGGLQNRVSPTPVGG--HQLDPFLVXXXXXXXXXXXXXPSPSVQVQTPRTSSSI 577
             +SQ  QGG Q R +     G      P +              P P VQ QT RT S+ 
Sbjct: 715  SRSQVQQGGAQGRATHAVGTGISQNAQPMVAAQRAAQMTRM---PLP-VQNQTSRTGSAF 770

Query: 578  HVNIDGYRPSGG----NTRVPVQPVTRPDGLVDSSVAEQDWRPTGRMRGSLSGQAYSAAL 745
             VN +G R + G    N    VQ V+RP+ LVD + +EQ+WRPTG MRGSL G+AY++AL
Sbjct: 771  PVNANGGRSTAGEQRGNIEGMVQAVSRPESLVDLA-SEQNWRPTGLMRGSLVGRAYNSAL 829

Query: 746  SQYISLPTQPVQPAARPLASLSTSLSAVPS--PPH 844
            +Q +  PTQP Q    P     T +++ P   PPH
Sbjct: 830  NQLVIQPTQPTQSTRPP-----TPITSPPPGFPPH 859