BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Acanthopanax21_contig00015986
         (2350 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_017241792.1| PREDICTED: isoamylase 1, chloroplastic [Dauc...  1228   0.0  
ref|XP_016454560.1| PREDICTED: isoamylase 1, chloroplastic-like ...  1226   0.0  
ref|XP_009588914.1| PREDICTED: isoamylase 1, chloroplastic [Nico...  1222   0.0  
ref|XP_019234068.1| PREDICTED: isoamylase 1, chloroplastic [Nico...  1221   0.0  
ref|XP_007014466.2| PREDICTED: isoamylase 1, chloroplastic isofo...  1215   0.0  
gb|EOY32085.1| Isoamylase 1 isoform 2 [Theobroma cacao]              1213   0.0  
ref|XP_009771255.1| PREDICTED: isoamylase 1, chloroplastic [Nico...  1212   0.0  
ref|XP_016434247.1| PREDICTED: isoamylase 1, chloroplastic-like ...  1210   0.0  
ref|XP_012075534.1| isoamylase 1, chloroplastic [Jatropha curcas...  1209   0.0  
ref|NP_001274937.1| isoamylase isoform 1 [Solanum tuberosum] >gi...  1207   0.0  
ref|XP_015082414.1| PREDICTED: isoamylase 1, chloroplastic isofo...  1206   0.0  
ref|XP_015881794.1| PREDICTED: isoamylase 1, chloroplastic [Zizi...  1205   0.0  
ref|XP_021277024.1| isoamylase 1, chloroplastic isoform X1 [Herr...  1204   0.0  
ref|XP_022731828.1| isoamylase 1, chloroplastic isoform X1 [Duri...  1203   0.0  
ref|XP_004242868.1| PREDICTED: isoamylase 1, chloroplastic isofo...  1203   0.0  
dbj|BAW33106.1| isoamylase [Ipomoea batatas]                         1202   0.0  
ref|XP_019188755.1| PREDICTED: isoamylase 1, chloroplastic [Ipom...  1202   0.0  
ref|XP_022037404.1| isoamylase 1, chloroplastic [Helianthus annu...  1200   0.0  
ref|XP_011094068.1| isoamylase 1, chloroplastic isoform X2 [Sesa...  1200   0.0  
ref|XP_002265964.3| PREDICTED: isoamylase 1, chloroplastic [Viti...  1199   0.0  

>ref|XP_017241792.1| PREDICTED: isoamylase 1, chloroplastic [Daucus carota subsp. sativus]
          Length = 795

 Score = 1228 bits (3176), Expect = 0.0
 Identities = 568/643 (88%), Positives = 601/643 (93%), Gaps = 1/643 (0%)
 Frame = +2

Query: 2    TNKTGDVWHVFVEG-DFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGV 178
            TNKTG+VWHVF+ G DF GMLY YKFDG +SP +GHYFDSS +L+DPY KAV SRGEFGV
Sbjct: 135  TNKTGNVWHVFLRGHDFEGMLYAYKFDGIFSPHQGHYFDSSNLLIDPYCKAVASRGEFGV 194

Query: 179  LGPGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGS 358
            LGPGDDCW QMAC++P+ADD+FDWEGDLPLKLQQKD VIYEMHVRGFT+HESS  + PGS
Sbjct: 195  LGPGDDCWTQMACQIPTADDEFDWEGDLPLKLQQKDQVIYEMHVRGFTKHESSNIKHPGS 254

Query: 359  YLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMAR 538
            YLGVVEKLDHLKELGVNCIELMPC EFNELEYYSYNSVLGDY+MNFWGYSTVNYFSPMAR
Sbjct: 255  YLGVVEKLDHLKELGVNCIELMPCQEFNELEYYSYNSVLGDYKMNFWGYSTVNYFSPMAR 314

Query: 539  YSTAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIF 718
            YS AGVH+ GLGA +EFK LVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNS++
Sbjct: 315  YSAAGVHNFGLGATDEFKLLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSVY 374

Query: 719  YMLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSL 898
            YMLAPKGEFYNYSGCGNT NCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASI+TRGSSL
Sbjct: 375  YMLAPKGEFYNYSGCGNTINCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASIMTRGSSL 434

Query: 899  WDAVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPH 1078
             DAVN+YG+Q E D LTTGSPL NPPLID+ISNDPILRGVKLIAEAWDCGGLYQVG FPH
Sbjct: 435  CDAVNIYGDQSEDDKLTTGSPLCNPPLIDLISNDPILRGVKLIAEAWDCGGLYQVGMFPH 494

Query: 1079 WGIWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGF 1258
            WGIWSEWNGKYRD IRQFIKGTDGFSGAFAECLCGSPNLYQE GRKPW+SINFI AHDGF
Sbjct: 495  WGIWSEWNGKYRDTIRQFIKGTDGFSGAFAECLCGSPNLYQEAGRKPWHSINFISAHDGF 554

Query: 1259 TLADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMV 1438
            TLADLVTYN+KHNLANGEDN DGENHNNSWNCGQEGEFAS SVK+LRKRQMRNLFLCL+V
Sbjct: 555  TLADLVTYNDKHNLANGEDNNDGENHNNSWNCGQEGEFASFSVKRLRKRQMRNLFLCLIV 614

Query: 1439 SQGVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECE 1618
            SQGVPMI+MGDEYGHTKGGNNNTYS DNYINYF WDKKDESS +FFRFCCLMTKFR+ECE
Sbjct: 615  SQGVPMIHMGDEYGHTKGGNNNTYSHDNYINYFRWDKKDESSCDFFRFCCLMTKFRRECE 674

Query: 1619 SLGLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLP 1798
            SLGLNDFPTAERLQWHGHSPGVPDWSD SRFVAFS MD +KGELYVAFNTSHLPVT MLP
Sbjct: 675  SLGLNDFPTAERLQWHGHSPGVPDWSDASRFVAFSFMDSLKGELYVAFNTSHLPVTVMLP 734

Query: 1799 ERPGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            ERPG+ W+PLVDT KP PFDFLSDDLLD+EVAIKQYS FL+AN
Sbjct: 735  ERPGFIWKPLVDTSKPAPFDFLSDDLLDKEVAIKQYSQFLEAN 777


>ref|XP_016454560.1| PREDICTED: isoamylase 1, chloroplastic-like [Nicotiana tabacum]
          Length = 793

 Score = 1226 bits (3173), Expect = 0.0
 Identities = 556/641 (86%), Positives = 602/641 (93%)
 Frame = +2

Query: 5    NKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVLG 184
            NKTGDVWHVF++GDF  MLYGYKFDGK+SPEEGH+FDSSQI+LDPYAKA VSRGE+GVLG
Sbjct: 133  NKTGDVWHVFLKGDFENMLYGYKFDGKFSPEEGHFFDSSQIVLDPYAKATVSRGEYGVLG 192

Query: 185  PGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSYL 364
            P DDCWP MA  VPSA D+FDWEGDLPLK  Q+D+VIYEMHVRG+T HESS T+FPG+Y 
Sbjct: 193  PADDCWPPMAGMVPSAADQFDWEGDLPLKFPQRDVVIYEMHVRGYTNHESSGTKFPGTYR 252

Query: 365  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARYS 544
            GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDY+ NFWGYSTVN+FSPM RYS
Sbjct: 253  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWGYSTVNFFSPMGRYS 312

Query: 545  TAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFYM 724
            +AG+ +CGLGA+NEFK+LV+EAHKRGIEV+MDVVFNHTAEGNENGPI+SFRGVDNS+FYM
Sbjct: 313  SAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGPIVSFRGVDNSVFYM 372

Query: 725  LAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLWD 904
            LAPKGEFYNYSGCGNTFNCNHPIVRQFI+DCLRYWV E+HVDGFRFDLASILTR SSLWD
Sbjct: 373  LAPKGEFYNYSGCGNTFNCNHPIVRQFIVDCLRYWVTEMHVDGFRFDLASILTRSSSLWD 432

Query: 905  AVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG 1084
            AVNVYGN  +VD++TTG+PL++PPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG
Sbjct: 433  AVNVYGNSTDVDVITTGTPLTSPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG 492

Query: 1085 IWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFTL 1264
            IWSEWNGKYRDI+RQFIKGTDGFSGA AECLCGSPNLYQEGGR PWNSINF+CAHDGFTL
Sbjct: 493  IWSEWNGKYRDIVRQFIKGTDGFSGALAECLCGSPNLYQEGGRNPWNSINFVCAHDGFTL 552

Query: 1265 ADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVSQ 1444
            ADLVTYN+KHNLANGEDNKDGENHNNSWNCG+EGEFAS+SVKKLRKRQMRN FLCLMVSQ
Sbjct: 553  ADLVTYNDKHNLANGEDNKDGENHNNSWNCGEEGEFASISVKKLRKRQMRNFFLCLMVSQ 612

Query: 1445 GVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECESL 1624
            GVPMIYMGDEYGHTKGGNNNTY  DNYINYF WDKKDESSSEFFRFCCL+TKFR ECESL
Sbjct: 613  GVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKDESSSEFFRFCCLVTKFRHECESL 672

Query: 1625 GLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPER 1804
            GL+ FPTAERLQWHGH+PG PDWS+ SRFVAF+L+D VKGELYVAFN SHLPVT  LP+R
Sbjct: 673  GLDGFPTAERLQWHGHAPGTPDWSETSRFVAFTLVDKVKGELYVAFNASHLPVTITLPDR 732

Query: 1805 PGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            PGYRW+PLVDTGKP P+DFL+DDL  RE A KQYSHFLDAN
Sbjct: 733  PGYRWQPLVDTGKPAPYDFLTDDLPGRETAAKQYSHFLDAN 773


>ref|XP_009588914.1| PREDICTED: isoamylase 1, chloroplastic [Nicotiana tomentosiformis]
          Length = 793

 Score = 1222 bits (3163), Expect = 0.0
 Identities = 555/641 (86%), Positives = 600/641 (93%)
 Frame = +2

Query: 5    NKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVLG 184
            NKTGDVWHVF++GDF  MLYGYKFDGK+SPEEGH+FDSSQI+LDPYAKA VSRGE+GVLG
Sbjct: 133  NKTGDVWHVFLKGDFENMLYGYKFDGKFSPEEGHFFDSSQIVLDPYAKATVSRGEYGVLG 192

Query: 185  PGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSYL 364
            P DDCWP MA  VPSA D+FDWEGDLPLK  Q+D+VIYEMHVRG+T HESS T+FPG+Y 
Sbjct: 193  PEDDCWPPMAGMVPSAADQFDWEGDLPLKFPQRDVVIYEMHVRGYTNHESSGTKFPGTYR 252

Query: 365  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARYS 544
            GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDY+ NFWGYSTVN+FSPM RYS
Sbjct: 253  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWGYSTVNFFSPMGRYS 312

Query: 545  TAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFYM 724
            +AG+ +CGLGA+NEFK+LV+EAHKRGIEV+MDVVFNHTAEGNENGPI+SFRGVDNS+FYM
Sbjct: 313  SAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGPIVSFRGVDNSVFYM 372

Query: 725  LAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLWD 904
            LAPKGEFYNYSGCGNTFNCNHPIVRQFI+DCLRYWV E+HVDGFRFDLASILTR SSLWD
Sbjct: 373  LAPKGEFYNYSGCGNTFNCNHPIVRQFIVDCLRYWVTEMHVDGFRFDLASILTRSSSLWD 432

Query: 905  AVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG 1084
            AVNVYGN  +VD++TTG+PL+NPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG
Sbjct: 433  AVNVYGNSTDVDVITTGTPLTNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG 492

Query: 1085 IWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFTL 1264
            IWSEWNGKYRDI+RQFIKGTDGFSGA AECLCGSPNLYQEGGR PWNSINF+CAHDGFTL
Sbjct: 493  IWSEWNGKYRDIVRQFIKGTDGFSGALAECLCGSPNLYQEGGRNPWNSINFVCAHDGFTL 552

Query: 1265 ADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVSQ 1444
            ADLVTYN+KHNLANGEDNKDGENHNNSWNCG+EGEFAS+SVKKLRKRQMRN FLCLMVSQ
Sbjct: 553  ADLVTYNDKHNLANGEDNKDGENHNNSWNCGEEGEFASISVKKLRKRQMRNFFLCLMVSQ 612

Query: 1445 GVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECESL 1624
            GVPMI MGDEYGHTKGGNNNTY  DNYINYF WDKKDESSS+FFRFCCL+TKFR ECESL
Sbjct: 613  GVPMICMGDEYGHTKGGNNNTYCHDNYINYFRWDKKDESSSDFFRFCCLVTKFRHECESL 672

Query: 1625 GLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPER 1804
            GL  FPTAERLQWHGH+PG PDWS+ SRFVAF+L+D VKGELYVAFN SHLPVT  LP+R
Sbjct: 673  GLGGFPTAERLQWHGHAPGTPDWSETSRFVAFTLVDKVKGELYVAFNASHLPVTITLPDR 732

Query: 1805 PGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            PGYRW+PLVDTGKP P+DFL+DDL  RE A KQYSHFLDAN
Sbjct: 733  PGYRWQPLVDTGKPAPYDFLTDDLPGRETAAKQYSHFLDAN 773


>ref|XP_019234068.1| PREDICTED: isoamylase 1, chloroplastic [Nicotiana attenuata]
 gb|OIT26982.1| isoamylase 1, chloroplastic [Nicotiana attenuata]
          Length = 794

 Score = 1221 bits (3158), Expect = 0.0
 Identities = 554/641 (86%), Positives = 602/641 (93%)
 Frame = +2

Query: 5    NKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVLG 184
            NKTGDVWHVF++GDF  MLYGYKFDGK+SPEEGH+FDSSQI+LDPYAKA VSRGE+GVLG
Sbjct: 134  NKTGDVWHVFLKGDFENMLYGYKFDGKFSPEEGHFFDSSQIVLDPYAKATVSRGEYGVLG 193

Query: 185  PGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSYL 364
            P DDCWP MA  VPSA D+FDWEGDLPLK  Q+DLVIYEMHVRG+T HESS T+FPG+Y 
Sbjct: 194  PEDDCWPPMAGMVPSAADQFDWEGDLPLKFPQRDLVIYEMHVRGYTNHESSGTKFPGTYR 253

Query: 365  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARYS 544
            GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDY++NFWGYSTVN+FSPM RYS
Sbjct: 254  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKLNFWGYSTVNFFSPMGRYS 313

Query: 545  TAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFYM 724
            +AG+ +CGLGA+NEFK+LV+EAHKRGIEV+MDVVFNHTAEGNENGPI+SFRG+DNS+FYM
Sbjct: 314  SAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGPIVSFRGIDNSVFYM 373

Query: 725  LAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLWD 904
            LAPKGEFYNYSGCGNTFNCNHPIVRQFI+DCLRYWV E+HVDGFRFDLASIL R SSLWD
Sbjct: 374  LAPKGEFYNYSGCGNTFNCNHPIVRQFIVDCLRYWVTEMHVDGFRFDLASILARSSSLWD 433

Query: 905  AVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG 1084
            AVNVYGN  +VD++TTG+PL++PPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG
Sbjct: 434  AVNVYGNSTDVDVITTGTPLTSPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG 493

Query: 1085 IWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFTL 1264
            IWSEWNGKYRDI+RQFIKGTDGFSGA AECLCGSPNLYQEGGRKP NSINF+CAHDGFTL
Sbjct: 494  IWSEWNGKYRDIVRQFIKGTDGFSGALAECLCGSPNLYQEGGRKPSNSINFVCAHDGFTL 553

Query: 1265 ADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVSQ 1444
            ADLVTYN+KHNLANGEDNKDGENHNNSWNCG+EGEFAS+SVKKLRKRQMRN FLCLMVSQ
Sbjct: 554  ADLVTYNDKHNLANGEDNKDGENHNNSWNCGEEGEFASISVKKLRKRQMRNFFLCLMVSQ 613

Query: 1445 GVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECESL 1624
            GVPMIYMGDEYGHTKGGNNNTY  DNYINYF WDKKDESSS+FFRFCCL+TKFR ECESL
Sbjct: 614  GVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKDESSSDFFRFCCLVTKFRHECESL 673

Query: 1625 GLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPER 1804
            GL+ FPTAERLQWHGH+PG PDWS+ SRFVAF+L+D VKGELYVAFN SHLPVT  LP+R
Sbjct: 674  GLDGFPTAERLQWHGHAPGTPDWSETSRFVAFTLVDKVKGELYVAFNASHLPVTITLPDR 733

Query: 1805 PGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            PGYRW+PLVDTGKP P+DFL+DDL  RE A KQYSHFLDAN
Sbjct: 734  PGYRWQPLVDTGKPAPYDFLTDDLPGRETAAKQYSHFLDAN 774


>ref|XP_007014466.2| PREDICTED: isoamylase 1, chloroplastic isoform X1 [Theobroma cacao]
          Length = 789

 Score = 1215 bits (3143), Expect = 0.0
 Identities = 543/642 (84%), Positives = 605/642 (94%)
 Frame = +2

Query: 2    TNKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVL 181
            TNKTGDVWHVF+ GDF  MLYGY FDGK+SPE+GHY+DSS+ILLDPYAKAV+SRGEFG L
Sbjct: 128  TNKTGDVWHVFLRGDFKDMLYGYIFDGKFSPEKGHYYDSSKILLDPYAKAVISRGEFGAL 187

Query: 182  GPGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSY 361
            GP D+CWPQMAC VP+++D+FDWEGDLPL+  Q+DL+IYEMHVRGFTRHESSRTEFPG+Y
Sbjct: 188  GPEDNCWPQMACMVPTSEDEFDWEGDLPLRYSQRDLIIYEMHVRGFTRHESSRTEFPGTY 247

Query: 362  LGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARY 541
             GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNS+LGDY++NFWGYSTVN+FSPM RY
Sbjct: 248  RGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSILGDYKLNFWGYSTVNFFSPMIRY 307

Query: 542  STAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFY 721
            S++G+H+CG  AVNEFK+LV+EAHKRGIEV MDVVFNHTAEGNENGPILSFRGVDNS++Y
Sbjct: 308  SSSGMHNCGRDAVNEFKYLVKEAHKRGIEVFMDVVFNHTAEGNENGPILSFRGVDNSVYY 367

Query: 722  MLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLW 901
            MLAPKGE+YNYSGCGNTFNCNHP+VRQFILDCLRYWV E+HVDGFRFDLASI+TR SSLW
Sbjct: 368  MLAPKGEYYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLASIMTRSSSLW 427

Query: 902  DAVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHW 1081
            D VNV+GN ++ D++TTG+PLS+PPLIDM+SNDP+LRGVKLIAEAWD GGLYQVG+FPHW
Sbjct: 428  DLVNVFGNPLKGDLITTGTPLSSPPLIDMMSNDPVLRGVKLIAEAWDTGGLYQVGSFPHW 487

Query: 1082 GIWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFT 1261
            GIWSEWNGKYRDI+RQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNS+NF+CAHDGFT
Sbjct: 488  GIWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSVNFVCAHDGFT 547

Query: 1262 LADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVS 1441
            LADLVTYN KHNLANGEDN DGE+HNNSWNCGQEGEFAS+SVKKLRKRQMRN FLCLMVS
Sbjct: 548  LADLVTYNNKHNLANGEDNNDGESHNNSWNCGQEGEFASISVKKLRKRQMRNFFLCLMVS 607

Query: 1442 QGVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECES 1621
            QG+PMI MGDEYGHTKGGNNNTY  DNYINYF WDKK+E SS+FFRFCCLMTKFR+ECES
Sbjct: 608  QGIPMICMGDEYGHTKGGNNNTYCHDNYINYFRWDKKEECSSDFFRFCCLMTKFRRECES 667

Query: 1622 LGLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPE 1801
            LGLNDFPTAERLQWHGH+PG+PDWSD+SRFVAF+L+D VKGE+YVAFNTS+LPVT  LPE
Sbjct: 668  LGLNDFPTAERLQWHGHTPGMPDWSDRSRFVAFTLIDSVKGEIYVAFNTSYLPVTITLPE 727

Query: 1802 RPGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            RPGYRW PLVDT KP PFDFLS++L +R++AIKQY  FLDAN
Sbjct: 728  RPGYRWEPLVDTSKPAPFDFLSEELKERDIAIKQYVQFLDAN 769


>gb|EOY32085.1| Isoamylase 1 isoform 2 [Theobroma cacao]
          Length = 795

 Score = 1213 bits (3139), Expect = 0.0
 Identities = 543/642 (84%), Positives = 604/642 (94%)
 Frame = +2

Query: 2    TNKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVL 181
            TNKTGDVWHVF+ GDF  MLYGY FDGK+SPE+GHY+DSS+ILLDPYAKAV+SRGEFG L
Sbjct: 134  TNKTGDVWHVFLRGDFKDMLYGYIFDGKFSPEKGHYYDSSKILLDPYAKAVISRGEFGAL 193

Query: 182  GPGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSY 361
            GP D+CWPQMAC VP+++D+FDWEGDLPL+  Q+DL+IYEMHVRGFTRHESSRTEFPG+Y
Sbjct: 194  GPEDNCWPQMACMVPTSEDEFDWEGDLPLRYSQRDLIIYEMHVRGFTRHESSRTEFPGTY 253

Query: 362  LGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARY 541
             GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNS+LGDY++NFWGYSTVN+FSPM RY
Sbjct: 254  RGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSILGDYKLNFWGYSTVNFFSPMIRY 313

Query: 542  STAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFY 721
            S++G+H+CG  AVNEFK+LV+EAHKRGIEV MDVVFNHTAEGNENGPILSFRGVDNS++Y
Sbjct: 314  SSSGMHNCGRDAVNEFKYLVKEAHKRGIEVFMDVVFNHTAEGNENGPILSFRGVDNSVYY 373

Query: 722  MLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLW 901
            MLAPKGE+YNYSGCGNTFNCNHP+VRQFILDCLRYWV E+HVDGFRFDLASI+TR SSLW
Sbjct: 374  MLAPKGEYYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLASIMTRSSSLW 433

Query: 902  DAVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHW 1081
            D VNV+GN ++ D++TTG+PLS+PPLIDM+SNDP+LRGVKLIAEAWD GGLYQVG+FPHW
Sbjct: 434  DLVNVFGNPLKGDLITTGTPLSSPPLIDMMSNDPVLRGVKLIAEAWDTGGLYQVGSFPHW 493

Query: 1082 GIWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFT 1261
            GIWSEWNGKYRDI+RQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINF+CAHDGFT
Sbjct: 494  GIWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFVCAHDGFT 553

Query: 1262 LADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVS 1441
            LADLVTYN KHNLANGEDN DGE+HNNSWNCGQEGEFAS+SVKKLRKRQMRN FLCLMVS
Sbjct: 554  LADLVTYNNKHNLANGEDNNDGESHNNSWNCGQEGEFASISVKKLRKRQMRNFFLCLMVS 613

Query: 1442 QGVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECES 1621
            QG+PMI MGDEYGHTKGGNNNTY  DNYINYF WDKK+E SS+FFRFCCLMTKFR+ECES
Sbjct: 614  QGIPMICMGDEYGHTKGGNNNTYCHDNYINYFQWDKKEECSSDFFRFCCLMTKFRRECES 673

Query: 1622 LGLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPE 1801
            LGLNDFPTAERLQWHGH+PG+PDWSD+SRFVAF+L+D VKGE+YVAFNTS+LPVT  LPE
Sbjct: 674  LGLNDFPTAERLQWHGHTPGMPDWSDRSRFVAFTLIDSVKGEIYVAFNTSYLPVTITLPE 733

Query: 1802 RPGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
             PGYRW PLVDT KP PFDFLS++L +R++AIKQY  FLDAN
Sbjct: 734  HPGYRWEPLVDTSKPAPFDFLSEELKERDIAIKQYVQFLDAN 775


>ref|XP_009771255.1| PREDICTED: isoamylase 1, chloroplastic [Nicotiana sylvestris]
          Length = 792

 Score = 1212 bits (3137), Expect = 0.0
 Identities = 551/641 (85%), Positives = 600/641 (93%)
 Frame = +2

Query: 5    NKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVLG 184
            NKTGDVWHVF++GDF  MLYGYKFDGK+SPE GH+FDSSQI+LDPYAKA VSRGE+GVLG
Sbjct: 133  NKTGDVWHVFLKGDFENMLYGYKFDGKFSPE-GHFFDSSQIVLDPYAKATVSRGEYGVLG 191

Query: 185  PGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSYL 364
            P DDCWP MA  VPSA D+FDWEGDLPLK  Q+DLVIYEMHVRG+T HESS T+FPG+Y 
Sbjct: 192  PEDDCWPLMAGMVPSAADQFDWEGDLPLKFPQRDLVIYEMHVRGYTNHESSGTKFPGTYR 251

Query: 365  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARYS 544
            GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDY+ NFWGYSTVN+FSPM RYS
Sbjct: 252  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWGYSTVNFFSPMGRYS 311

Query: 545  TAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFYM 724
            +AG+ +CGL A+NEFK+LV+EAHKRGIEV+MDVVFNHTAEGNENGPI+SFRG+DNS+FYM
Sbjct: 312  SAGLSNCGLVAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGPIVSFRGIDNSVFYM 371

Query: 725  LAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLWD 904
            LAPKGEFYNYSGCGNTFNCNHPIVRQFI+DCLRYWV E+H+DGFRFDLASILTR SSLWD
Sbjct: 372  LAPKGEFYNYSGCGNTFNCNHPIVRQFIVDCLRYWVTEMHIDGFRFDLASILTRSSSLWD 431

Query: 905  AVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG 1084
            AVNVYGN  +VD++TTG+PL++PPLIDMISNDPILRGVKLIAEAWDCGGLYQVG FPHWG
Sbjct: 432  AVNVYGNSTDVDVITTGTPLTSPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGMFPHWG 491

Query: 1085 IWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFTL 1264
            IWSEWNGKYRDI+RQFIKGTDGFSGA AECLCGSPNLYQEGGRKPWNSINF+CAHDGFTL
Sbjct: 492  IWSEWNGKYRDIVRQFIKGTDGFSGALAECLCGSPNLYQEGGRKPWNSINFVCAHDGFTL 551

Query: 1265 ADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVSQ 1444
            ADLVTYN+KHNLANGEDNKDGENHNNSWNCG+EGEFAS+SVKKLRKRQMRN FLCLMVSQ
Sbjct: 552  ADLVTYNDKHNLANGEDNKDGENHNNSWNCGEEGEFASISVKKLRKRQMRNFFLCLMVSQ 611

Query: 1445 GVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECESL 1624
            GVPMIYMGDEYGHTKGGNNNTY  DNYINYF WDKKDESSS+FFRFCCL+TKFR +CESL
Sbjct: 612  GVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKDESSSDFFRFCCLVTKFRHKCESL 671

Query: 1625 GLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPER 1804
            GL+ FPTAERLQWHGH+PG PDWS+ SRFVAF+L+D VKGELYVAFN SHLPVT  LP+R
Sbjct: 672  GLDGFPTAERLQWHGHAPGTPDWSETSRFVAFTLVDKVKGELYVAFNASHLPVTITLPDR 731

Query: 1805 PGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            PGYRW+PLVDTGKP P+DFL+DDL  RE A KQYSHFLDAN
Sbjct: 732  PGYRWQPLVDTGKPAPYDFLTDDLPGRETAAKQYSHFLDAN 772


>ref|XP_016434247.1| PREDICTED: isoamylase 1, chloroplastic-like [Nicotiana tabacum]
          Length = 792

 Score = 1210 bits (3131), Expect = 0.0
 Identities = 550/641 (85%), Positives = 599/641 (93%)
 Frame = +2

Query: 5    NKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVLG 184
            NKTGDVWHVF++GDF  MLYGYKFDGK+SPE GH+FDSSQI+LDPYAKA VSRGE+GVLG
Sbjct: 133  NKTGDVWHVFLKGDFENMLYGYKFDGKFSPE-GHFFDSSQIVLDPYAKATVSRGEYGVLG 191

Query: 185  PGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSYL 364
            P DDCWP MA  VPSA D+FDWEGDLPLK  Q+DLVIYEMHVRG+T HESS T+FPG+Y 
Sbjct: 192  PEDDCWPLMAGMVPSAADQFDWEGDLPLKFPQRDLVIYEMHVRGYTNHESSGTKFPGTYR 251

Query: 365  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARYS 544
            GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDY+ NFWGYSTVN+FSPM RYS
Sbjct: 252  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWGYSTVNFFSPMGRYS 311

Query: 545  TAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFYM 724
            +AG+ +CGL A+NEFK+LV+EAHKRGIEV+MDVVFNHTAEGNENGPI+SFRG+DNS+FYM
Sbjct: 312  SAGLSNCGLVAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGPIVSFRGIDNSVFYM 371

Query: 725  LAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLWD 904
            LAPKGEFYNYSGCGNTFNCNHPIVRQFI+DCLRYWV E+H+DGFRFDLASILTR SSLWD
Sbjct: 372  LAPKGEFYNYSGCGNTFNCNHPIVRQFIVDCLRYWVTEMHIDGFRFDLASILTRSSSLWD 431

Query: 905  AVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG 1084
            AVNVYGN  +VD++TTG+PL++PPLIDMISNDPILRGVKLIAEAWDCGGLYQVG FPHWG
Sbjct: 432  AVNVYGNSTDVDVITTGTPLTSPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGMFPHWG 491

Query: 1085 IWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFTL 1264
            IWSEWNGKYRDI+RQFIKGTDGFSGA AECLCGSPNLYQEGGRKPWNSINF+CAHDGFTL
Sbjct: 492  IWSEWNGKYRDIVRQFIKGTDGFSGALAECLCGSPNLYQEGGRKPWNSINFVCAHDGFTL 551

Query: 1265 ADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVSQ 1444
            ADLVTYN+KHNLANGEDNKDGENH NSWNCG+EGEFAS+SVKKLRKRQMRN FLCLMVSQ
Sbjct: 552  ADLVTYNDKHNLANGEDNKDGENHKNSWNCGEEGEFASISVKKLRKRQMRNFFLCLMVSQ 611

Query: 1445 GVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECESL 1624
            GVPMIYMGDEYGHTKGGNNNTY  DNYINYF WDKKDESSS+FFRFCCL+TKFR +CESL
Sbjct: 612  GVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKDESSSDFFRFCCLVTKFRHKCESL 671

Query: 1625 GLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPER 1804
            GL+ FPTAERLQWHGH+PG PDWS+ SRFVAF+L+D VKGELYVAFN SHLPVT  LP+R
Sbjct: 672  GLDGFPTAERLQWHGHAPGTPDWSETSRFVAFTLVDKVKGELYVAFNASHLPVTITLPDR 731

Query: 1805 PGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            PGYRW+PLVDTGKP P+DFL+DDL  RE A KQYSHFLDAN
Sbjct: 732  PGYRWQPLVDTGKPAPYDFLTDDLPGRETAAKQYSHFLDAN 772


>ref|XP_012075534.1| isoamylase 1, chloroplastic [Jatropha curcas]
 gb|KDP34860.1| hypothetical protein JCGZ_09148 [Jatropha curcas]
          Length = 793

 Score = 1209 bits (3128), Expect = 0.0
 Identities = 552/642 (85%), Positives = 598/642 (93%)
 Frame = +2

Query: 2    TNKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVL 181
            TNKTGD+WHVF++GDF  MLYGY+FDGK SPEEGHYF+SS+I+LDPYAKAV+SRGEFGVL
Sbjct: 132  TNKTGDIWHVFLKGDFKDMLYGYRFDGKLSPEEGHYFESSEIVLDPYAKAVISRGEFGVL 191

Query: 182  GPGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSY 361
            GP D+CWPQMA  +P+A DKFDWEGDLPLK  QKDL+IYEMHVRGFT+HESSRTEFPG+Y
Sbjct: 192  GPDDNCWPQMAGMIPAARDKFDWEGDLPLKYPQKDLMIYEMHVRGFTQHESSRTEFPGTY 251

Query: 362  LGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARY 541
            LGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDY+MNFWGYSTVNYFSPM RY
Sbjct: 252  LGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKMNFWGYSTVNYFSPMTRY 311

Query: 542  STAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFY 721
            S+AG  +CG  A+NEFK LVREAHKRGIEV+MDVVFNHTAEGNE GPILSFRGVDNS++Y
Sbjct: 312  SSAGTRNCGHDAINEFKLLVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGVDNSVYY 371

Query: 722  MLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLW 901
            MLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWV+E+HVDGFRFDLASI+TRGSSLW
Sbjct: 372  MLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVIEMHVDGFRFDLASIMTRGSSLW 431

Query: 902  DAVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHW 1081
            DAVNV+GN +E D+LTTG+PLS+PPLIDMISNDPILR VKLIAEAWD GGLYQVG FPHW
Sbjct: 432  DAVNVFGNPVEGDLLTTGTPLSSPPLIDMISNDPILREVKLIAEAWDAGGLYQVGCFPHW 491

Query: 1082 GIWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFT 1261
             IWSEWNGKYRDI+RQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINF+CAHDGFT
Sbjct: 492  QIWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFVCAHDGFT 551

Query: 1262 LADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVS 1441
            LADLVTYN K+NLANGEDN DGENHNNSWNCG EGEFAS+SVKKLRKRQMRN F+CLMVS
Sbjct: 552  LADLVTYNNKNNLANGEDNNDGENHNNSWNCGLEGEFASISVKKLRKRQMRNFFVCLMVS 611

Query: 1442 QGVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECES 1621
            QGVPMIYMGDEYGHTKGGNNNTY  DNYINYF WDKK+ESSS+F+RFCCLMTKFR ECES
Sbjct: 612  QGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKEESSSDFYRFCCLMTKFRSECES 671

Query: 1622 LGLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPE 1801
            LGLNDFPTAERLQWHGH P +PDWS+ SRFVAF++ D VKGE+YVAFN  HLPVT  LPE
Sbjct: 672  LGLNDFPTAERLQWHGHVPRMPDWSETSRFVAFTVTDTVKGEIYVAFNAKHLPVTVALPE 731

Query: 1802 RPGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            R GYRW PLVDTGK +PFDFLS D+ +RE+AIKQYSHFLDAN
Sbjct: 732  RLGYRWEPLVDTGKASPFDFLSGDVPEREIAIKQYSHFLDAN 773


>ref|NP_001274937.1| isoamylase isoform 1 [Solanum tuberosum]
 gb|AAN15317.1| isoamylase isoform 1 [Solanum tuberosum]
          Length = 793

 Score = 1207 bits (3122), Expect = 0.0
 Identities = 546/641 (85%), Positives = 597/641 (93%)
 Frame = +2

Query: 5    NKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVLG 184
            NKTGDVWHVF++GDF  MLYGYKFDGK+ PEEGHYFDSSQI+LDPYAKA+VSRGE+GVLG
Sbjct: 133  NKTGDVWHVFLKGDFENMLYGYKFDGKFCPEEGHYFDSSQIVLDPYAKAIVSRGEYGVLG 192

Query: 185  PGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSYL 364
            P DDCWP MA  VPSA D+FDWEGDLPLK  Q+DLVIYEMHVRGFT HESS T++PG+YL
Sbjct: 193  PEDDCWPPMAGMVPSASDQFDWEGDLPLKFPQRDLVIYEMHVRGFTNHESSETKYPGTYL 252

Query: 365  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARYS 544
            GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDY+ NFWGYSTVN+FSPM RYS
Sbjct: 253  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWGYSTVNFFSPMGRYS 312

Query: 545  TAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFYM 724
            +AG+ +CGLGA+NEFK+LV+EAHKRGIEV+MDVVFNHTAEGNENGPILSFRG+DNS+FY 
Sbjct: 313  SAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGPILSFRGIDNSVFYT 372

Query: 725  LAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLWD 904
            LAPKGEFYNYSGCGNTFNCN+PIVRQFI+DCLRYWV E+HVDGFRFDLASILTR SS W+
Sbjct: 373  LAPKGEFYNYSGCGNTFNCNNPIVRQFIVDCLRYWVTEMHVDGFRFDLASILTRSSSSWN 432

Query: 905  AVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG 1084
            AVNVYGN ++ D++TTG+PL++PPLIDMISNDPILRGVKLIAEAWDCGGLYQVG FPHWG
Sbjct: 433  AVNVYGNSIDGDVITTGTPLTSPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGMFPHWG 492

Query: 1085 IWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFTL 1264
            IWSEWNGKYRD++RQFIKGTDGFSGAFAECLCGSPNLYQ+GGRKPWNSINF+CAHDGFTL
Sbjct: 493  IWSEWNGKYRDMVRQFIKGTDGFSGAFAECLCGSPNLYQKGGRKPWNSINFVCAHDGFTL 552

Query: 1265 ADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVSQ 1444
            ADLVTYN KHNLANGEDNKDGENHNNSWNCG+EGEFAS+ VKKLRKRQMRN FLCLMVSQ
Sbjct: 553  ADLVTYNNKHNLANGEDNKDGENHNNSWNCGEEGEFASIFVKKLRKRQMRNFFLCLMVSQ 612

Query: 1445 GVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECESL 1624
            GVPMIYMGDEYGHTKGGNNNTY  DNYINYF WDKKDESSS+F RFC LMTKFR ECESL
Sbjct: 613  GVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKDESSSDFLRFCGLMTKFRHECESL 672

Query: 1625 GLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPER 1804
            GL+ FPTAERLQWHGH+P  PDWS+ SRFVAF+L+D VKGELY+AFN SHLPVT  LP+R
Sbjct: 673  GLDGFPTAERLQWHGHTPRTPDWSETSRFVAFTLVDKVKGELYIAFNASHLPVTITLPDR 732

Query: 1805 PGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            PGYRW+P VDTGKP PFDFL+DD+ +RE A KQYSHFLDAN
Sbjct: 733  PGYRWQPFVDTGKPAPFDFLTDDVPERETAAKQYSHFLDAN 773


>ref|XP_015082414.1| PREDICTED: isoamylase 1, chloroplastic isoform X1 [Solanum pennellii]
 ref|XP_015082415.1| PREDICTED: isoamylase 1, chloroplastic isoform X2 [Solanum pennellii]
          Length = 787

 Score = 1206 bits (3121), Expect = 0.0
 Identities = 546/641 (85%), Positives = 597/641 (93%)
 Frame = +2

Query: 5    NKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVLG 184
            NKTGDVWHVF++GDF  MLYGYKFDGK+ PEEGHYFDSSQI+LDPYAKA+VSRGE+GVLG
Sbjct: 127  NKTGDVWHVFLKGDFENMLYGYKFDGKFCPEEGHYFDSSQIVLDPYAKAIVSRGEYGVLG 186

Query: 185  PGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSYL 364
            P DDCWP MA  VPSA D+FDWEGDLPLK  Q+DLVIYEMHVRGFT HESS T++PG+YL
Sbjct: 187  PEDDCWPPMAGMVPSASDQFDWEGDLPLKFPQRDLVIYEMHVRGFTNHESSETKYPGTYL 246

Query: 365  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARYS 544
            GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDY+ NFWGYSTVN+FSPM RYS
Sbjct: 247  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWGYSTVNFFSPMGRYS 306

Query: 545  TAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFYM 724
            +AG+ +CGLGA+NEFK+LV+EAHKRGIEV+MDVVFNHTAEGNENGPILSFRG+DNS+FY 
Sbjct: 307  SAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGPILSFRGIDNSVFYT 366

Query: 725  LAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLWD 904
            LAPKGEFYNYSGCGNTFNCN+PIVRQFI+DCLRYWV E+HVDGFRFDLASILTR SS W+
Sbjct: 367  LAPKGEFYNYSGCGNTFNCNNPIVRQFIVDCLRYWVTEMHVDGFRFDLASILTRSSSSWN 426

Query: 905  AVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG 1084
            AVNVYGN ++ D++TTG+PL++PPLIDMISNDPILRGVKLIAEAWDCGGLYQVG FPHWG
Sbjct: 427  AVNVYGNSIDGDVITTGTPLTSPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGMFPHWG 486

Query: 1085 IWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFTL 1264
            IWSEWNGKYRD++RQFIKGTDGFSGAFAECLCGSPNLYQ+GGRKPWNSINF+CAHDGFTL
Sbjct: 487  IWSEWNGKYRDMVRQFIKGTDGFSGAFAECLCGSPNLYQKGGRKPWNSINFVCAHDGFTL 546

Query: 1265 ADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVSQ 1444
            ADLVTYN KHNLANGEDNKDGENHNNSWNCG+EGEFAS+ VKKLRKRQMRN FLCLMVSQ
Sbjct: 547  ADLVTYNNKHNLANGEDNKDGENHNNSWNCGEEGEFASIFVKKLRKRQMRNFFLCLMVSQ 606

Query: 1445 GVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECESL 1624
            GVPMIYMGDEYGHTKGGNNNTY  DNYINYF WDKKDESSS+F RFC LMTKFR ECESL
Sbjct: 607  GVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKDESSSDFLRFCSLMTKFRHECESL 666

Query: 1625 GLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPER 1804
            GL+ FPTAERLQWHGH+P  PDWS+ SRFVAF+L+D VKGELY+AFN SHLPVT  LPER
Sbjct: 667  GLDGFPTAERLQWHGHTPRTPDWSETSRFVAFTLVDKVKGELYIAFNASHLPVTITLPER 726

Query: 1805 PGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            PGY W+PLVDTGKP PFDFL+DD+ +R+ A KQYSHFLDAN
Sbjct: 727  PGYLWQPLVDTGKPAPFDFLTDDVPERDTAAKQYSHFLDAN 767


>ref|XP_015881794.1| PREDICTED: isoamylase 1, chloroplastic [Ziziphus jujuba]
          Length = 675

 Score = 1205 bits (3117), Expect = 0.0
 Identities = 543/642 (84%), Positives = 599/642 (93%)
 Frame = +2

Query: 2    TNKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVL 181
            TNK+G+VWHVF++GDF  MLYGYKFDGK SPE+GHY+D S+I+LDPYAKAV+ RG+FG L
Sbjct: 15   TNKSGNVWHVFLKGDFDNMLYGYKFDGKLSPEKGHYYDYSRIVLDPYAKAVIRRGDFGTL 74

Query: 182  GPGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSY 361
            GP  DCWPQMAC VPS++ KFDWEGDLPLK  QKDL+IYEMHVRGFTRHESSRTEFPG+Y
Sbjct: 75   GPDGDCWPQMACAVPSSNSKFDWEGDLPLKYPQKDLIIYEMHVRGFTRHESSRTEFPGTY 134

Query: 362  LGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARY 541
             GVVEKLDHLKELG+NCIELMPC+EFNELEY+SYNSVLGDY++N+WGYSTV+YFSPM RY
Sbjct: 135  RGVVEKLDHLKELGINCIELMPCNEFNELEYFSYNSVLGDYKVNYWGYSTVSYFSPMIRY 194

Query: 542  STAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFY 721
            S+AGV +CG  A+NE K L+REAHKRGIEV+MDVVFNHTAEGNENGPILSFRG DNS++Y
Sbjct: 195  SSAGVSNCGYDAINEVKLLIREAHKRGIEVIMDVVFNHTAEGNENGPILSFRGADNSVYY 254

Query: 722  MLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLW 901
            MLAPKGEFYNYSGCGNTFNCNHPIVRQFI+DCLRYWV E+HVDGFRFDLASI+TRGSSLW
Sbjct: 255  MLAPKGEFYNYSGCGNTFNCNHPIVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLW 314

Query: 902  DAVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHW 1081
            DAVNVYGN +E D+LTTG+PLS+PPLIDMISNDPILRGVKLIAEAWD GGLYQVG FPHW
Sbjct: 315  DAVNVYGNPIEGDLLTTGTPLSSPPLIDMISNDPILRGVKLIAEAWDTGGLYQVGKFPHW 374

Query: 1082 GIWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFT 1261
            G+WSEWNGKYRDI+RQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINF+CAHDGFT
Sbjct: 375  GVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFVCAHDGFT 434

Query: 1262 LADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVS 1441
            LADLV+YN+KHNLANGEDN DGENHNNSWNCGQEGEF S+SVKKLRKRQMRN FLCLMVS
Sbjct: 435  LADLVSYNKKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFLCLMVS 494

Query: 1442 QGVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECES 1621
            QGVPMIYMGDEYGHTKGGNNNTY  DNYINYF WDKK++SSS+FFRFCCLMTKFRQ+CES
Sbjct: 495  QGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKEDSSSDFFRFCCLMTKFRQDCES 554

Query: 1622 LGLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPE 1801
            LGL+DFPTAERLQWHGH+P  PDWS+KSRFVAFSLMD VKGE+Y+AFNTSHL VT  LPE
Sbjct: 555  LGLHDFPTAERLQWHGHAPRTPDWSEKSRFVAFSLMDSVKGEIYIAFNTSHLAVTISLPE 614

Query: 1802 RPGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            RPG+RW PLVDT KP P+DFLS DL  R++AI+QY+HFLDAN
Sbjct: 615  RPGHRWEPLVDTSKPAPYDFLSSDLPGRDIAIQQYAHFLDAN 656


>ref|XP_021277024.1| isoamylase 1, chloroplastic isoform X1 [Herrania umbratica]
          Length = 794

 Score = 1204 bits (3116), Expect = 0.0
 Identities = 540/642 (84%), Positives = 601/642 (93%)
 Frame = +2

Query: 2    TNKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVL 181
            TNK GDVWHVF+ GDF  MLYGY FDGK+SP++GHY+DSS+ILLDPYAKAV+SRGEFG L
Sbjct: 133  TNKIGDVWHVFLRGDFKDMLYGYIFDGKFSPKKGHYYDSSKILLDPYAKAVISRGEFGAL 192

Query: 182  GPGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSY 361
            GP D+CWPQMAC VP+++D+FDWEGDLPL+  Q+DL+IYEMHVRGFTRHESSRTEFPG+Y
Sbjct: 193  GPEDNCWPQMACMVPTSEDEFDWEGDLPLRYSQRDLIIYEMHVRGFTRHESSRTEFPGTY 252

Query: 362  LGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARY 541
             GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDY++NFWGYSTVNYFSPM RY
Sbjct: 253  HGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKLNFWGYSTVNYFSPMIRY 312

Query: 542  STAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFY 721
            S++G+ +CG  A+NEFK+LV+EAHKRGIEV MDVVFNHTAEGNENGPILSFRGVDNS++Y
Sbjct: 313  SSSGMCNCGRDAINEFKYLVKEAHKRGIEVFMDVVFNHTAEGNENGPILSFRGVDNSVYY 372

Query: 722  MLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLW 901
            MLAPKGE+YNYSGCGNTFNCNHP+VRQFILDCLRYWV E+HVDGFRFDLASI+TR SSLW
Sbjct: 373  MLAPKGEYYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLASIMTRSSSLW 432

Query: 902  DAVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHW 1081
            D VNV+GN +E D++TTG+PL++PPLIDM+SNDP+LRGVKLIAEAWD GGLYQVG+FPHW
Sbjct: 433  DLVNVFGNPLEGDLITTGTPLNSPPLIDMMSNDPVLRGVKLIAEAWDTGGLYQVGSFPHW 492

Query: 1082 GIWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFT 1261
            GIWSEWNGKYRDI+RQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINF+CAHDGF+
Sbjct: 493  GIWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFVCAHDGFS 552

Query: 1262 LADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVS 1441
            LADLVTYN KHNLANGEDN DGE+HNNSWNCGQEGEFA +SVKKLRKRQMRN FLCLMVS
Sbjct: 553  LADLVTYNNKHNLANGEDNNDGESHNNSWNCGQEGEFARISVKKLRKRQMRNFFLCLMVS 612

Query: 1442 QGVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECES 1621
            QG+PMI MGDEYGHTKGGNNNTY  DNYINYF WDKK ESSS+FFRFCCLMT FR+ECES
Sbjct: 613  QGIPMICMGDEYGHTKGGNNNTYCHDNYINYFRWDKKKESSSDFFRFCCLMTNFRRECES 672

Query: 1622 LGLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPE 1801
            LGLNDFPTAERLQWHGH+PG+PDWSDKSRFVAF+L+D VKGE+YVAFNTS+LPVT  LPE
Sbjct: 673  LGLNDFPTAERLQWHGHTPGMPDWSDKSRFVAFTLIDSVKGEIYVAFNTSYLPVTITLPE 732

Query: 1802 RPGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            RPGYRW PLVDT KP PFDFLS++L +R++AIKQY+ FLD N
Sbjct: 733  RPGYRWEPLVDTSKPAPFDFLSNELKERDIAIKQYAQFLDVN 774


>ref|XP_022731828.1| isoamylase 1, chloroplastic isoform X1 [Durio zibethinus]
          Length = 789

 Score = 1203 bits (3113), Expect = 0.0
 Identities = 541/642 (84%), Positives = 598/642 (93%)
 Frame = +2

Query: 2    TNKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVL 181
            TNKTGDVWHVF+ GDF  MLYGY FDG++SPE+GHY+DSS+ILLDPYAKAV+SRGEFG L
Sbjct: 128  TNKTGDVWHVFLRGDFKDMLYGYIFDGEFSPEKGHYYDSSRILLDPYAKAVISRGEFGAL 187

Query: 182  GPGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSY 361
            GP D+CWPQMAC VP+++ +FDWEGDLPL+  Q+DL++YEMHVRGFTRHESSRTEFPG+Y
Sbjct: 188  GPEDNCWPQMACMVPTSEAQFDWEGDLPLRYPQRDLIVYEMHVRGFTRHESSRTEFPGTY 247

Query: 362  LGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARY 541
              VVEKLDHLKELGVNCIELMPCHEFNELE+YSYNSVLGDY++NFWGYSTVNYFSPM RY
Sbjct: 248  RAVVEKLDHLKELGVNCIELMPCHEFNELEHYSYNSVLGDYKLNFWGYSTVNYFSPMIRY 307

Query: 542  STAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFY 721
            S+AG+ + G  A+NEFKHLV+EAHKRGIEV MDVVFNHTAEGNE GPILSFRGVDNS++Y
Sbjct: 308  SSAGIDNYGRDAINEFKHLVKEAHKRGIEVFMDVVFNHTAEGNEKGPILSFRGVDNSVYY 367

Query: 722  MLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLW 901
            MLAPKGE+YNYSGCGNT NCNHP+VRQFILDCLRYWV E+HVDGFRFDLASI+TR SSLW
Sbjct: 368  MLAPKGEYYNYSGCGNTLNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLASIMTRSSSLW 427

Query: 902  DAVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHW 1081
            D+VNVYGN +E D++TTG+PLS+PPLIDM+SNDP+LRGVKLIAEAWD GGLYQVGTFPHW
Sbjct: 428  DSVNVYGNTLEGDLITTGTPLSSPPLIDMLSNDPVLRGVKLIAEAWDTGGLYQVGTFPHW 487

Query: 1082 GIWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFT 1261
            GIWSEWNGKYRDI+RQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNS+NF+CAHDGFT
Sbjct: 488  GIWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSVNFVCAHDGFT 547

Query: 1262 LADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVS 1441
            LADLVTYN KHNLANGEDN DGE+HNNSWNCGQEGEFAS+SVKKLRKRQMRN FLCLMVS
Sbjct: 548  LADLVTYNNKHNLANGEDNNDGESHNNSWNCGQEGEFASISVKKLRKRQMRNFFLCLMVS 607

Query: 1442 QGVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECES 1621
            QGVPMI MGDEYGHTKGGNNNTY  DN INYF WDKK+ESSS+FFRFCCLMTKFR ECES
Sbjct: 608  QGVPMICMGDEYGHTKGGNNNTYCHDNNINYFRWDKKEESSSDFFRFCCLMTKFRHECES 667

Query: 1622 LGLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPE 1801
            LGLNDFPTAERLQWHGH+PG+PDWSD SRFVAF+++D VKGE+YVAFNTSHLPVT  LPE
Sbjct: 668  LGLNDFPTAERLQWHGHNPGMPDWSDASRFVAFTMIDSVKGEIYVAFNTSHLPVTITLPE 727

Query: 1802 RPGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            RPGYRW PLVDT KP PFDFLS +L +R++AIKQY+ FLDAN
Sbjct: 728  RPGYRWEPLVDTSKPVPFDFLSSELPERDIAIKQYAQFLDAN 769


>ref|XP_004242868.1| PREDICTED: isoamylase 1, chloroplastic isoform X2 [Solanum
            lycopersicum]
          Length = 787

 Score = 1203 bits (3112), Expect = 0.0
 Identities = 544/641 (84%), Positives = 596/641 (92%)
 Frame = +2

Query: 5    NKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVLG 184
            NKTGDVWHVF++GDF  MLYGYKFDGK+ PEEGHYFDSSQI+LDPYAKA+VSRGE+GVLG
Sbjct: 127  NKTGDVWHVFLKGDFENMLYGYKFDGKFCPEEGHYFDSSQIVLDPYAKAIVSRGEYGVLG 186

Query: 185  PGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSYL 364
            P DDCWP MA  VPSA D+FDWEGDLPLK  Q++LVIYEMHVRGFT HESS T++PG+YL
Sbjct: 187  PEDDCWPPMAGMVPSASDQFDWEGDLPLKFSQRNLVIYEMHVRGFTNHESSETKYPGTYL 246

Query: 365  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARYS 544
            GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDY+ NFWGYSTVN+FSPM RYS
Sbjct: 247  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWGYSTVNFFSPMGRYS 306

Query: 545  TAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFYM 724
            +AG+ +CGLGA+NEFK+LV+EAHKRGIEV+MDVVFNHTAEGNENGPILSFRG+DNS+FY 
Sbjct: 307  SAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGPILSFRGIDNSVFYT 366

Query: 725  LAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLWD 904
            LAPKGEFYNYSGCGNTFNCN+PIVRQFI+DCLRYWV E+HVDGFRFDLASILTR SS W+
Sbjct: 367  LAPKGEFYNYSGCGNTFNCNNPIVRQFIVDCLRYWVTEMHVDGFRFDLASILTRSSSSWN 426

Query: 905  AVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG 1084
            AVNVYGN ++ D++TTG+PL++PPLIDMISNDPILRGVKLIAEAWDCGGLYQVG FPHWG
Sbjct: 427  AVNVYGNSIDGDVITTGTPLTSPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGMFPHWG 486

Query: 1085 IWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFTL 1264
            IWSEWNGKYRD++RQFIKGTDGFSGAFAECLCGSPNLYQ+GGRKPWNSINF+CAHDGFTL
Sbjct: 487  IWSEWNGKYRDMVRQFIKGTDGFSGAFAECLCGSPNLYQKGGRKPWNSINFVCAHDGFTL 546

Query: 1265 ADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVSQ 1444
            ADLVTYN KHNLANGEDNKDGENHNNSWNCG+EGEFAS+ VKKLRKRQMRN FLCLMVSQ
Sbjct: 547  ADLVTYNNKHNLANGEDNKDGENHNNSWNCGEEGEFASIFVKKLRKRQMRNFFLCLMVSQ 606

Query: 1445 GVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECESL 1624
            GVPMIYMGDEYGHTKGGNNNTY  DNYINYF WDKKDESSS+F RFC LMT FR ECESL
Sbjct: 607  GVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKDESSSDFLRFCSLMTNFRHECESL 666

Query: 1625 GLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPER 1804
            GL+ FPTAERLQWHGH+P  PDWS+ SRFVAF+L+D VKGELY+AFN SHLPVT  LPER
Sbjct: 667  GLDGFPTAERLQWHGHTPRTPDWSETSRFVAFTLVDKVKGELYIAFNASHLPVTITLPER 726

Query: 1805 PGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            PGY W+PLVDTGKP PFDFL+DD+ +R+ A KQYSHFLDAN
Sbjct: 727  PGYLWQPLVDTGKPAPFDFLTDDVPERDTAAKQYSHFLDAN 767


>dbj|BAW33106.1| isoamylase [Ipomoea batatas]
          Length = 799

 Score = 1202 bits (3110), Expect = 0.0
 Identities = 543/641 (84%), Positives = 594/641 (92%)
 Frame = +2

Query: 5    NKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVLG 184
            NKTGDVWHVF++GDF  MLYGY FDGK++PEEGHYFDS++ILLDPYAKA+VSR EFG LG
Sbjct: 137  NKTGDVWHVFLKGDFDNMLYGYSFDGKFAPEEGHYFDSARILLDPYAKAIVSRAEFGALG 196

Query: 185  PGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSYL 364
            P  DCWP MAC +PSAD KFDWEGDLPLK  Q+DLVIYEMHVRGFT HESS TEFPG+Y 
Sbjct: 197  PEKDCWPPMACMLPSAD-KFDWEGDLPLKFPQRDLVIYEMHVRGFTNHESSGTEFPGTYR 255

Query: 365  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARYS 544
            GVVEKLDHLK LGVNCIELMPCHEFNELEYYSYN VLGDY++NFWGYSTVN+FSPM RYS
Sbjct: 256  GVVEKLDHLKGLGVNCIELMPCHEFNELEYYSYNPVLGDYKVNFWGYSTVNFFSPMGRYS 315

Query: 545  TAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFYM 724
            +AG+H  GLGA++EFK+LVREAH+RGIEV+MDVVFNHTAEGNENGP+ SFRGVDNS+FYM
Sbjct: 316  SAGMHKSGLGAIDEFKYLVREAHRRGIEVIMDVVFNHTAEGNENGPMFSFRGVDNSVFYM 375

Query: 725  LAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLWD 904
            LAPKGEFYNYSGCGNTFNCNHP+ RQFILDCLRYWV+E+HVDGFRFDLASILTR SSLWD
Sbjct: 376  LAPKGEFYNYSGCGNTFNCNHPVARQFILDCLRYWVIEMHVDGFRFDLASILTRSSSLWD 435

Query: 905  AVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG 1084
            A NVYGN +E DMLTTG+PLS+PPLIDMIS+DPIL GVKLIAEAWDCGGLYQVG FPHWG
Sbjct: 436  AANVYGNSIEGDMLTTGTPLSSPPLIDMISSDPILSGVKLIAEAWDCGGLYQVGAFPHWG 495

Query: 1085 IWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFTL 1264
             WSEWNGKYRDI+RQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINF+CAHDGFTL
Sbjct: 496  FWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFVCAHDGFTL 555

Query: 1265 ADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVSQ 1444
            ADLVTYN+KHN+ANGEDNKDGENHNNSWNCGQEGEFAS+SVKKLRKRQ+RN FLCLMVSQ
Sbjct: 556  ADLVTYNDKHNMANGEDNKDGENHNNSWNCGQEGEFASISVKKLRKRQLRNFFLCLMVSQ 615

Query: 1445 GVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECESL 1624
            GVPMIYMGDEYGHTKGGNNNTY  DNYINYF WDKKDESS++FFRFCC MTKFR E ESL
Sbjct: 616  GVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKDESSTDFFRFCCRMTKFRHEAESL 675

Query: 1625 GLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPER 1804
            GL+DFPTAERLQWHGH+PG+PDWS+ SRFVAF+L+D VKGE+Y+AFN SHLPVT  LPER
Sbjct: 676  GLDDFPTAERLQWHGHTPGMPDWSESSRFVAFTLVDKVKGEIYIAFNASHLPVTVTLPER 735

Query: 1805 PGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
             GYRW PLVDTGK TPFDFL DD+ +++ A+KQY+HFLDAN
Sbjct: 736  GGYRWEPLVDTGKQTPFDFLGDDVPEKKTALKQYAHFLDAN 776


>ref|XP_019188755.1| PREDICTED: isoamylase 1, chloroplastic [Ipomoea nil]
          Length = 800

 Score = 1202 bits (3109), Expect = 0.0
 Identities = 543/641 (84%), Positives = 595/641 (92%)
 Frame = +2

Query: 5    NKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVLG 184
            NKTGDVWHVF++GDF  MLYGY FDGK++PEEGHYFDS++ILLDPYAKA+VSR EFG LG
Sbjct: 138  NKTGDVWHVFLKGDFDNMLYGYSFDGKFAPEEGHYFDSTRILLDPYAKAIVSRAEFGALG 197

Query: 185  PGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSYL 364
            P  DCW  MAC +PSAD+ FDWEGDLPLK  Q+DLVIYEMHVRGFT+HESS TEFPG+Y 
Sbjct: 198  PEKDCWSPMACMLPSADE-FDWEGDLPLKFPQRDLVIYEMHVRGFTKHESSGTEFPGTYR 256

Query: 365  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARYS 544
            GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYN VLGDY++NFWGYSTVN+FSPM RYS
Sbjct: 257  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNPVLGDYKVNFWGYSTVNFFSPMGRYS 316

Query: 545  TAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFYM 724
            +AG+H  GLGA++EFK+LVREAH+RGIEV+MDVVFNHTAEGNENGP+ SFRGVDNS+FYM
Sbjct: 317  SAGMHKSGLGAIDEFKYLVREAHRRGIEVIMDVVFNHTAEGNENGPMFSFRGVDNSVFYM 376

Query: 725  LAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLWD 904
            LAPKGEFYNYSGCGNTFNCNHP+ RQFILDCLRYWV+E+HVDGFRFDLASILTR SSLWD
Sbjct: 377  LAPKGEFYNYSGCGNTFNCNHPVARQFILDCLRYWVIEMHVDGFRFDLASILTRSSSLWD 436

Query: 905  AVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG 1084
            A NVYGN +E DMLTTG+PLS+PPLIDMIS+DPIL GVKLIAEAWDCGGLYQVG FPHWG
Sbjct: 437  AANVYGNSIEGDMLTTGTPLSSPPLIDMISSDPILTGVKLIAEAWDCGGLYQVGAFPHWG 496

Query: 1085 IWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFTL 1264
            IWSEWNGKYRDI+RQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNS+NF+CAHDGFTL
Sbjct: 497  IWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSVNFVCAHDGFTL 556

Query: 1265 ADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVSQ 1444
            ADLVTYN+KHN+ANGEDNKDGENHNNSWNCGQEGEFAS+SVKKLRKRQMRN FLCLMVSQ
Sbjct: 557  ADLVTYNDKHNMANGEDNKDGENHNNSWNCGQEGEFASISVKKLRKRQMRNFFLCLMVSQ 616

Query: 1445 GVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECESL 1624
            GVPMIYMGDEYGHTKGGNNNTY  DNYINYF WDKKDESS++FFRFCC MTKFR E ESL
Sbjct: 617  GVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKDESSTDFFRFCCHMTKFRHEAESL 676

Query: 1625 GLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPER 1804
            GL+DFPTAERLQWHGH+PGVPDWS+ SRFVAF+L+D VKGE+Y+AFN SHLPVT  LPER
Sbjct: 677  GLDDFPTAERLQWHGHAPGVPDWSEGSRFVAFTLVDKVKGEIYIAFNASHLPVTVTLPER 736

Query: 1805 PGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
             GYRW PLVDTGK  PFDFL DD+ +++ A+KQY+HFLDAN
Sbjct: 737  GGYRWEPLVDTGKQAPFDFLGDDVPEKKTALKQYAHFLDAN 777


>ref|XP_022037404.1| isoamylase 1, chloroplastic [Helianthus annuus]
 gb|OTG24410.1| putative isoamylase 1 [Helianthus annuus]
          Length = 789

 Score = 1200 bits (3104), Expect = 0.0
 Identities = 549/642 (85%), Positives = 596/642 (92%)
 Frame = +2

Query: 2    TNKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVL 181
            TNKTGDVWHVF++GDFT MLYGYKFDG++ PEEGHY+DSSQILLDPYAKAVVSRGEFGVL
Sbjct: 129  TNKTGDVWHVFLKGDFTDMLYGYKFDGEFCPEEGHYYDSSQILLDPYAKAVVSRGEFGVL 188

Query: 182  GPGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSY 361
            GP DDCW QMA  +PS D+ FDWEGDLPL   Q+DLVIYEMHVRGFTRHESS+TE  G+Y
Sbjct: 189  GPDDDCWSQMAGRIPSVDE-FDWEGDLPLAFAQRDLVIYEMHVRGFTRHESSKTESRGTY 247

Query: 362  LGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARY 541
            LGVV+KLDHLKELGVNCIELMPCHEFNELEY+SYN VLGDYR+N+WGYSTVNYFSPM+RY
Sbjct: 248  LGVVDKLDHLKELGVNCIELMPCHEFNELEYFSYNPVLGDYRLNYWGYSTVNYFSPMSRY 307

Query: 542  STAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFY 721
            ++AG  +CG+ A+NEFK LV+EAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNS+FY
Sbjct: 308  ASAGARNCGINAINEFKQLVKEAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSVFY 367

Query: 722  MLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLW 901
            MLAPKGEFYNYSGCGNTFNCNHP+VRQFI+D LRYWV E+HVDGFRFDLASILTRGSSLW
Sbjct: 368  MLAPKGEFYNYSGCGNTFNCNHPVVRQFIVDTLRYWVTEMHVDGFRFDLASILTRGSSLW 427

Query: 902  DAVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHW 1081
            D+VNVYGNQ+E D+LTTGSPLS+PPLIDMISNDPILRGVKLIAEAWDCGGLYQVG FPHW
Sbjct: 428  DSVNVYGNQLEDDLLTTGSPLSSPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGAFPHW 487

Query: 1082 GIWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFT 1261
            GIWSEWNGKYRD +RQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFT
Sbjct: 488  GIWSEWNGKYRDTVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFT 547

Query: 1262 LADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVS 1441
            LADLVTYN+KHNLANGEDNKDG++HNNSWNCGQEGEF S+SVK+LRKRQMRN FLCLMVS
Sbjct: 548  LADLVTYNDKHNLANGEDNKDGDSHNNSWNCGQEGEFVSISVKRLRKRQMRNFFLCLMVS 607

Query: 1442 QGVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECES 1621
            QGVPMI+MGDEYGHTKGGNNNTY  DN+INYF WDKK+ESSS+FFRF  L+T FR ECES
Sbjct: 608  QGVPMIHMGDEYGHTKGGNNNTYCHDNHINYFQWDKKEESSSDFFRFSRLITNFRHECES 667

Query: 1622 LGLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPE 1801
            LGLNDFPTAERLQWHGH P  PDWS+ SRFVAF++ D VKGE+Y+AFNTSHLPVT  LPE
Sbjct: 668  LGLNDFPTAERLQWHGHIPETPDWSETSRFVAFTMKDSVKGEVYIAFNTSHLPVTITLPE 727

Query: 1802 RPGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            RPGYRW PLVDT KP PFDFLS D+  REVAIKQY+HFLDAN
Sbjct: 728  RPGYRWDPLVDTSKPAPFDFLSHDVPGREVAIKQYAHFLDAN 769


>ref|XP_011094068.1| isoamylase 1, chloroplastic isoform X2 [Sesamum indicum]
          Length = 798

 Score = 1200 bits (3104), Expect = 0.0
 Identities = 539/641 (84%), Positives = 596/641 (92%)
 Frame = +2

Query: 5    NKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVLG 184
            N+TGDVWHVF++GDF  M+YGY+FDG +SPE+G YFDSS+IL+DPYAKAVVSRGE+G LG
Sbjct: 138  NRTGDVWHVFLKGDFEDMVYGYRFDGSFSPEDGLYFDSSKILIDPYAKAVVSRGEYGALG 197

Query: 185  PGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSYL 364
            P ++CWPQMAC VPS +D+FDWEGDLPLK  Q+DLVIYEMHVRGFTRHESS ++FPG+Y 
Sbjct: 198  PDEECWPQMACVVPSINDEFDWEGDLPLKFPQRDLVIYEMHVRGFTRHESSNSKFPGTYR 257

Query: 365  GVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARYS 544
            G+VEKLDHLK+LG+NCIELMPCHEFNELEYYSYNSVLGDY++NFWGYST+NYFSPMARYS
Sbjct: 258  GLVEKLDHLKKLGINCIELMPCHEFNELEYYSYNSVLGDYKVNFWGYSTINYFSPMARYS 317

Query: 545  TAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFYM 724
            +AGV   GLGAVNEFK+LVREAHKRGIEV+MDVVFNHTAEGNENGPILSFRG+DNS+FYM
Sbjct: 318  SAGVGKYGLGAVNEFKYLVREAHKRGIEVIMDVVFNHTAEGNENGPILSFRGLDNSVFYM 377

Query: 725  LAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLWD 904
            LAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWV E+HVDGFRFDLASILTR SSLWD
Sbjct: 378  LAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVTEMHVDGFRFDLASILTRSSSLWD 437

Query: 905  AVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHWG 1084
            AVNVYG+ +E + +T G+PLS+PPLIDMISNDPIL GVKLIAEAWDCGGLYQVG FPHWG
Sbjct: 438  AVNVYGDAIEGEAITIGTPLSSPPLIDMISNDPILHGVKLIAEAWDCGGLYQVGIFPHWG 497

Query: 1085 IWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFTL 1264
            IWSEWNGKYRDI+RQFIKGTDGF+GAFAECLCGSPNLYQEGGRKPWNSINF+CAHDGFTL
Sbjct: 498  IWSEWNGKYRDIVRQFIKGTDGFAGAFAECLCGSPNLYQEGGRKPWNSINFVCAHDGFTL 557

Query: 1265 ADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVSQ 1444
            ADLVTYN KHNLANGEDNKDGENHNNSWNCGQEGEFAS+SVKKLR+RQMRN FLCLMVSQ
Sbjct: 558  ADLVTYNSKHNLANGEDNKDGENHNNSWNCGQEGEFASISVKKLRRRQMRNFFLCLMVSQ 617

Query: 1445 GVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECESL 1624
            GVPMIYMGDEYGHTKGGNNNTY  DN+INYF WDK +E+ S+FFR+CCLMTKFR ECESL
Sbjct: 618  GVPMIYMGDEYGHTKGGNNNTYCHDNFINYFRWDKMEEAESDFFRYCCLMTKFRHECESL 677

Query: 1625 GLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPER 1804
            GLNDFPTAERLQWHGH+P  PDWSD SRFVAF+L+D VKGELY+AFN SH+ VT  LPER
Sbjct: 678  GLNDFPTAERLQWHGHTPATPDWSDTSRFVAFTLIDSVKGELYIAFNASHIGVTVALPER 737

Query: 1805 PGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            PGYRW PLVDT K  P+DFLSDDL DR+ A++QY+HFLD+N
Sbjct: 738  PGYRWEPLVDTSKAAPYDFLSDDLADRDTAVRQYAHFLDSN 778


>ref|XP_002265964.3| PREDICTED: isoamylase 1, chloroplastic [Vitis vinifera]
          Length = 793

 Score = 1199 bits (3103), Expect = 0.0
 Identities = 542/642 (84%), Positives = 598/642 (93%)
 Frame = +2

Query: 2    TNKTGDVWHVFVEGDFTGMLYGYKFDGKYSPEEGHYFDSSQILLDPYAKAVVSRGEFGVL 181
            TNKTGDVWHVF++G+F  ++YGYKFDGK+SPEEGHY+DSS++LLDPYAKAV+SRGEFG+L
Sbjct: 131  TNKTGDVWHVFLKGNFENIVYGYKFDGKFSPEEGHYYDSSRLLLDPYAKAVISRGEFGIL 190

Query: 182  GPGDDCWPQMACEVPSADDKFDWEGDLPLKLQQKDLVIYEMHVRGFTRHESSRTEFPGSY 361
            GP  +CWP MA  +PS+D +FDWEGDLPLK  QKDL+IYEMHVRGFTRHESSRT+FPG+Y
Sbjct: 191  GPEGNCWPLMAGMIPSSDAEFDWEGDLPLKYPQKDLIIYEMHVRGFTRHESSRTKFPGTY 250

Query: 362  LGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYRMNFWGYSTVNYFSPMARY 541
             GVVEKLDHLKELGVNCIELMPCHEFNELEY+SYNSVL DYR+NFWGYSTVNYFSPM RY
Sbjct: 251  HGVVEKLDHLKELGVNCIELMPCHEFNELEYFSYNSVLDDYRVNFWGYSTVNYFSPMIRY 310

Query: 542  STAGVHSCGLGAVNEFKHLVREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSIFY 721
            S+AG+H+CG  A+NE K L+REAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNS++Y
Sbjct: 311  SSAGIHNCGHDAINEVKLLIREAHKRGIEVLMDVVFNHTAEGNENGPILSFRGVDNSVYY 370

Query: 722  MLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVMEIHVDGFRFDLASILTRGSSLW 901
            MLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWV E+HVDGFRFDLASI+TRGSSLW
Sbjct: 371  MLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVTEMHVDGFRFDLASIMTRGSSLW 430

Query: 902  DAVNVYGNQMEVDMLTTGSPLSNPPLIDMISNDPILRGVKLIAEAWDCGGLYQVGTFPHW 1081
            DA+NVYGN  E D LTTG+PLS+PPLIDMISNDPILRGVKLIAEAWD GGLYQVG FPHW
Sbjct: 431  DALNVYGNPAEGDSLTTGTPLSSPPLIDMISNDPILRGVKLIAEAWDAGGLYQVGMFPHW 490

Query: 1082 GIWSEWNGKYRDIIRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFICAHDGFT 1261
            G+WSEWNGKYRDI+RQFIKG+DGFSGAFAECLCGSPNLYQEGGRKPWNSINF+CAHDGFT
Sbjct: 491  GLWSEWNGKYRDIVRQFIKGSDGFSGAFAECLCGSPNLYQEGGRKPWNSINFVCAHDGFT 550

Query: 1262 LADLVTYNEKHNLANGEDNKDGENHNNSWNCGQEGEFASLSVKKLRKRQMRNLFLCLMVS 1441
            LADLVTYN+KHN ANGEDN DGENHNNSWNCGQEGEFAS+SVKKLRKRQMRN FLCLMVS
Sbjct: 551  LADLVTYNKKHNNANGEDNNDGENHNNSWNCGQEGEFASISVKKLRKRQMRNFFLCLMVS 610

Query: 1442 QGVPMIYMGDEYGHTKGGNNNTYSLDNYINYFHWDKKDESSSEFFRFCCLMTKFRQECES 1621
            QGVPMIYMGDEYGHTKGGNNNTY  DNY+NYF WDKK+ES S+FFRFCCLM+KFRQECES
Sbjct: 611  QGVPMIYMGDEYGHTKGGNNNTYCQDNYMNYFRWDKKEESLSDFFRFCCLMSKFRQECES 670

Query: 1622 LGLNDFPTAERLQWHGHSPGVPDWSDKSRFVAFSLMDLVKGELYVAFNTSHLPVTAMLPE 1801
            LGLNDFPTAERLQWHG +PG+PDWS  SRFVAF+++D VKGE+YVAFNTSHLP+   LPE
Sbjct: 671  LGLNDFPTAERLQWHGRTPGMPDWSKTSRFVAFTMVDSVKGEIYVAFNTSHLPIIITLPE 730

Query: 1802 RPGYRWRPLVDTGKPTPFDFLSDDLLDREVAIKQYSHFLDAN 1927
            RPGYRW+PLVDT KP PFDFLS+D+ +R+ A+KQYS F +AN
Sbjct: 731  RPGYRWQPLVDTSKPAPFDFLSNDVPERDTAVKQYSQFTEAN 772