BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Acanthopanax21_contig00015446
         (2335 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_017253556.1| PREDICTED: pullulanase 1, chloroplastic isof...  1121   0.0  
ref|XP_017253554.1| PREDICTED: pullulanase 1, chloroplastic isof...  1121   0.0  
gb|KZM95827.1| hypothetical protein DCAR_019069 [Daucus carota s...  1121   0.0  
ref|XP_010658333.1| PREDICTED: pullulanase 1, chloroplastic isof...  1092   0.0  
ref|XP_002271820.3| PREDICTED: pullulanase 1, chloroplastic isof...  1092   0.0  
gb|AOQ26240.1| PUL1 [Actinidia deliciosa]                            1073   0.0  
gb|ONI23748.1| hypothetical protein PRUPE_2G205800 [Prunus persica]  1057   0.0  
ref|XP_007220271.1| pullulanase 1, chloroplastic [Prunus persica...  1057   0.0  
ref|XP_023879674.1| pullulanase 1, chloroplastic isoform X2 [Que...  1057   0.0  
ref|XP_023879673.1| pullulanase 1, chloroplastic isoform X1 [Que...  1057   0.0  
ref|XP_009587792.1| PREDICTED: pullulanase 1, chloroplastic isof...  1051   0.0  
ref|XP_009587786.1| PREDICTED: pullulanase 1, chloroplastic isof...  1051   0.0  
ref|XP_016443816.1| PREDICTED: pullulanase 1, chloroplastic-like...  1048   0.0  
ref|XP_009791833.1| PREDICTED: pullulanase 1, chloroplastic isof...  1048   0.0  
ref|XP_018808475.1| PREDICTED: pullulanase 1, chloroplastic isof...  1048   0.0  
ref|XP_018808474.1| PREDICTED: pullulanase 1, chloroplastic isof...  1048   0.0  
ref|XP_016436612.1| PREDICTED: pullulanase 1, chloroplastic-like...  1048   0.0  
ref|XP_021690508.1| pullulanase 1, chloroplastic isoform X1 [Hev...  1046   0.0  
ref|XP_019236140.1| PREDICTED: pullulanase 1, chloroplastic isof...  1045   0.0  
ref|XP_019236141.1| PREDICTED: pullulanase 1, chloroplastic isof...  1045   0.0  

>ref|XP_017253556.1| PREDICTED: pullulanase 1, chloroplastic isoform X3 [Daucus carota
            subsp. sativus]
          Length = 780

 Score = 1121 bits (2900), Expect = 0.0
 Identities = 536/619 (86%), Positives = 582/619 (94%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            SD TV P FRGGYLAFTSQ+SAGINHL+KLS AGITH+HLLP FQF GV+DEKDKWKSVD
Sbjct: 162  SDNTVDPEFRGGYLAFTSQESAGINHLRKLSKAGITHIHLLPTFQFDGVKDEKDKWKSVD 221

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
            TQMLESLPPDS +QQEYITAIQN+DGYNWGYNPVLWGVPKGSYASNPNGP RTIEFRKMV
Sbjct: 222  TQMLESLPPDSAQQQEYITAIQNEDGYNWGYNPVLWGVPKGSYASNPNGPLRTIEFRKMV 281

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NRIGLRVVLDVVYNHLQGSGPF +NSVLDKIVPGYYLR NTNG IE+S C+NNTASE
Sbjct: 282  QALNRIGLRVVLDVVYNHLQGSGPFGENSVLDKIVPGYYLRMNTNGQIENSACMNNTASE 341

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            HFMVERLI+DDLLCWAVDYKVDGFRFDLMGHIMKHTMVKA +ML+GLSK K GV+GA IY
Sbjct: 342  HFMVERLIIDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKANNMLRGLSKIKHGVEGAKIY 401

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVAKNGRGTN+SQFN+SNTGIGSFNDRIRDA+LGGSPFGHPLQQGFVTGL 
Sbjct: 402  IYGEGWDFGEVAKNGRGTNSSQFNISNTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLG 461

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            LQANG+DHGSKSD  RTLAV KDHIQVGMAANLKDYVL +CDG+EVKGSEV TYDGVPV 
Sbjct: 462  LQANGHDHGSKSDAVRTLAVSKDHIQVGMAANLKDYVLTDCDGEEVKGSEVKTYDGVPVA 521

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+S P+ETVNYVSAHDNETLFDI+SLKTPM+ISVDERCRINHLATSI+ALSQGIPFFH+G
Sbjct: 522  YASCPSETVNYVSAHDNETLFDIISLKTPMDISVDERCRINHLATSIVALSQGIPFFHAG 581

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNE +WPLIKPRLADPSFKP
Sbjct: 582  DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNENHWPLIKPRLADPSFKP 641

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
             + HILAAVE+ LNLL+IRYSSPLFRLR+ANA+QERVRF+NTGPSW+PG+IVMGIEDGHN
Sbjct: 642  CSRHILAAVEDMLNLLEIRYSSPLFRLRSANAVQERVRFYNTGPSWIPGLIVMGIEDGHN 701

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD+ Y YI+V++NVCPTEVSF+S ALRAK+L+LHPVQM STD IVKK+TYDAS
Sbjct: 702  GVPGLSQLDSIYCYIVVVINVCPTEVSFSSHALRAKSLQLHPVQMNSTDTIVKKTTYDAS 761

Query: 1801 SGYFKLPPRTTAIFVEPRN 1857
            +G FK+P RTTA+FVE R+
Sbjct: 762  AGRFKVPSRTTAVFVECRD 780


>ref|XP_017253554.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Daucus carota
            subsp. sativus]
          Length = 949

 Score = 1121 bits (2900), Expect = 0.0
 Identities = 536/619 (86%), Positives = 582/619 (94%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            SD TV P FRGGYLAFTSQ+SAGINHL+KLS AGITH+HLLP FQF GV+DEKDKWKSVD
Sbjct: 331  SDNTVDPEFRGGYLAFTSQESAGINHLRKLSKAGITHIHLLPTFQFDGVKDEKDKWKSVD 390

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
            TQMLESLPPDS +QQEYITAIQN+DGYNWGYNPVLWGVPKGSYASNPNGP RTIEFRKMV
Sbjct: 391  TQMLESLPPDSAQQQEYITAIQNEDGYNWGYNPVLWGVPKGSYASNPNGPLRTIEFRKMV 450

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NRIGLRVVLDVVYNHLQGSGPF +NSVLDKIVPGYYLR NTNG IE+S C+NNTASE
Sbjct: 451  QALNRIGLRVVLDVVYNHLQGSGPFGENSVLDKIVPGYYLRMNTNGQIENSACMNNTASE 510

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            HFMVERLI+DDLLCWAVDYKVDGFRFDLMGHIMKHTMVKA +ML+GLSK K GV+GA IY
Sbjct: 511  HFMVERLIIDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKANNMLRGLSKIKHGVEGAKIY 570

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVAKNGRGTN+SQFN+SNTGIGSFNDRIRDA+LGGSPFGHPLQQGFVTGL 
Sbjct: 571  IYGEGWDFGEVAKNGRGTNSSQFNISNTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLG 630

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            LQANG+DHGSKSD  RTLAV KDHIQVGMAANLKDYVL +CDG+EVKGSEV TYDGVPV 
Sbjct: 631  LQANGHDHGSKSDAVRTLAVSKDHIQVGMAANLKDYVLTDCDGEEVKGSEVKTYDGVPVA 690

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+S P+ETVNYVSAHDNETLFDI+SLKTPM+ISVDERCRINHLATSI+ALSQGIPFFH+G
Sbjct: 691  YASCPSETVNYVSAHDNETLFDIISLKTPMDISVDERCRINHLATSIVALSQGIPFFHAG 750

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNE +WPLIKPRLADPSFKP
Sbjct: 751  DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNENHWPLIKPRLADPSFKP 810

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
             + HILAAVE+ LNLL+IRYSSPLFRLR+ANA+QERVRF+NTGPSW+PG+IVMGIEDGHN
Sbjct: 811  CSRHILAAVEDMLNLLEIRYSSPLFRLRSANAVQERVRFYNTGPSWIPGLIVMGIEDGHN 870

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD+ Y YI+V++NVCPTEVSF+S ALRAK+L+LHPVQM STD IVKK+TYDAS
Sbjct: 871  GVPGLSQLDSIYCYIVVVINVCPTEVSFSSHALRAKSLQLHPVQMNSTDTIVKKTTYDAS 930

Query: 1801 SGYFKLPPRTTAIFVEPRN 1857
            +G FK+P RTTA+FVE R+
Sbjct: 931  AGRFKVPSRTTAVFVECRD 949


>gb|KZM95827.1| hypothetical protein DCAR_019069 [Daucus carota subsp. sativus]
          Length = 890

 Score = 1121 bits (2900), Expect = 0.0
 Identities = 536/619 (86%), Positives = 582/619 (94%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            SD TV P FRGGYLAFTSQ+SAGINHL+KLS AGITH+HLLP FQF GV+DEKDKWKSVD
Sbjct: 272  SDNTVDPEFRGGYLAFTSQESAGINHLRKLSKAGITHIHLLPTFQFDGVKDEKDKWKSVD 331

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
            TQMLESLPPDS +QQEYITAIQN+DGYNWGYNPVLWGVPKGSYASNPNGP RTIEFRKMV
Sbjct: 332  TQMLESLPPDSAQQQEYITAIQNEDGYNWGYNPVLWGVPKGSYASNPNGPLRTIEFRKMV 391

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NRIGLRVVLDVVYNHLQGSGPF +NSVLDKIVPGYYLR NTNG IE+S C+NNTASE
Sbjct: 392  QALNRIGLRVVLDVVYNHLQGSGPFGENSVLDKIVPGYYLRMNTNGQIENSACMNNTASE 451

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            HFMVERLI+DDLLCWAVDYKVDGFRFDLMGHIMKHTMVKA +ML+GLSK K GV+GA IY
Sbjct: 452  HFMVERLIIDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKANNMLRGLSKIKHGVEGAKIY 511

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVAKNGRGTN+SQFN+SNTGIGSFNDRIRDA+LGGSPFGHPLQQGFVTGL 
Sbjct: 512  IYGEGWDFGEVAKNGRGTNSSQFNISNTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLG 571

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            LQANG+DHGSKSD  RTLAV KDHIQVGMAANLKDYVL +CDG+EVKGSEV TYDGVPV 
Sbjct: 572  LQANGHDHGSKSDAVRTLAVSKDHIQVGMAANLKDYVLTDCDGEEVKGSEVKTYDGVPVA 631

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+S P+ETVNYVSAHDNETLFDI+SLKTPM+ISVDERCRINHLATSI+ALSQGIPFFH+G
Sbjct: 632  YASCPSETVNYVSAHDNETLFDIISLKTPMDISVDERCRINHLATSIVALSQGIPFFHAG 691

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNE +WPLIKPRLADPSFKP
Sbjct: 692  DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNENHWPLIKPRLADPSFKP 751

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
             + HILAAVE+ LNLL+IRYSSPLFRLR+ANA+QERVRF+NTGPSW+PG+IVMGIEDGHN
Sbjct: 752  CSRHILAAVEDMLNLLEIRYSSPLFRLRSANAVQERVRFYNTGPSWIPGLIVMGIEDGHN 811

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD+ Y YI+V++NVCPTEVSF+S ALRAK+L+LHPVQM STD IVKK+TYDAS
Sbjct: 812  GVPGLSQLDSIYCYIVVVINVCPTEVSFSSHALRAKSLQLHPVQMNSTDTIVKKTTYDAS 871

Query: 1801 SGYFKLPPRTTAIFVEPRN 1857
            +G FK+P RTTA+FVE R+
Sbjct: 872  AGRFKVPSRTTAVFVECRD 890


>ref|XP_010658333.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Vitis vinifera]
          Length = 954

 Score = 1092 bits (2825), Expect = 0.0
 Identities = 518/620 (83%), Positives = 567/620 (91%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            SD+TVHP+FRGGYLAFTSQDSAGI HLKKL NAGI+H+HLLP FQFAGV+DEKDKWK VD
Sbjct: 335  SDHTVHPDFRGGYLAFTSQDSAGILHLKKLCNAGISHLHLLPTFQFAGVDDEKDKWKCVD 394

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
              +LE+LPPDSVEQQ  I  IQ++DGYNWGYNPVLWGVPKGSYASNPN PCRT+EFRKMV
Sbjct: 395  PNILETLPPDSVEQQAQIMTIQDEDGYNWGYNPVLWGVPKGSYASNPNSPCRTLEFRKMV 454

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NRIG RVVLDVVYNHL GSGPFD+NSVLDKIVPGYYLRRN++G IEHS CVNNTASE
Sbjct: 455  QALNRIGFRVVLDVVYNHLHGSGPFDENSVLDKIVPGYYLRRNSDGCIEHSACVNNTASE 514

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            HFMVERLI+DDLLCWAVDYKVDGFRFDLMGHIMK TMVKAK+ML  LSKD  G+DG+SIY
Sbjct: 515  HFMVERLILDDLLCWAVDYKVDGFRFDLMGHIMKRTMVKAKNMLHSLSKDTDGIDGSSIY 574

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVAKNGRG NASQFNL  TGIGSFNDRIRDA+LGGSPFGHPLQQGFVTGL 
Sbjct: 575  IYGEGWDFGEVAKNGRGVNASQFNLCGTGIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLF 634

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            LQ N +DHG +   ER LAV KDHIQVGMAANLKD+VL NC+G+EVKGSEVSTYDG PV 
Sbjct: 635  LQPNDHDHGGEEVAERMLAVSKDHIQVGMAANLKDFVLTNCEGEEVKGSEVSTYDGTPVA 694

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+  PTET+NYVSAHDNETLFDIVSLKTPM+ISV+ERCRINHLATSIIALSQGIPFFHSG
Sbjct: 695  YTVCPTETINYVSAHDNETLFDIVSLKTPMQISVEERCRINHLATSIIALSQGIPFFHSG 754

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DEMLRSKS+DRDSYNSGDWFNRLDFSY+SNNWGVGLPPKEKNE+NWPLIKPRLADPSFKP
Sbjct: 755  DEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEKNWPLIKPRLADPSFKP 814

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
            Q  HI+AAVENFLNLL+IRYSSPLFRLRTAN+IQ RVRFHNTGPSWVPG+IVM IEDGH 
Sbjct: 815  QKNHIIAAVENFLNLLKIRYSSPLFRLRTANSIQARVRFHNTGPSWVPGIIVMSIEDGHE 874

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD  YSYI+VI+N  PTE+ FASPAL+A+TL+LHPVQ+MS+D ++K STY+AS
Sbjct: 875  GVPGLSQLDPVYSYIVVIINPSPTEIKFASPALQARTLQLHPVQVMSSDGVIKNSTYEAS 934

Query: 1801 SGYFKLPPRTTAIFVEPRNV 1860
            SG F +PPRTT++FVEPR +
Sbjct: 935  SGCFIVPPRTTSVFVEPREI 954


>ref|XP_002271820.3| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Vitis vinifera]
 emb|CBI31395.3| unnamed protein product, partial [Vitis vinifera]
          Length = 956

 Score = 1092 bits (2825), Expect = 0.0
 Identities = 518/620 (83%), Positives = 567/620 (91%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            SD+TVHP+FRGGYLAFTSQDSAGI HLKKL NAGI+H+HLLP FQFAGV+DEKDKWK VD
Sbjct: 337  SDHTVHPDFRGGYLAFTSQDSAGILHLKKLCNAGISHLHLLPTFQFAGVDDEKDKWKCVD 396

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
              +LE+LPPDSVEQQ  I  IQ++DGYNWGYNPVLWGVPKGSYASNPN PCRT+EFRKMV
Sbjct: 397  PNILETLPPDSVEQQAQIMTIQDEDGYNWGYNPVLWGVPKGSYASNPNSPCRTLEFRKMV 456

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NRIG RVVLDVVYNHL GSGPFD+NSVLDKIVPGYYLRRN++G IEHS CVNNTASE
Sbjct: 457  QALNRIGFRVVLDVVYNHLHGSGPFDENSVLDKIVPGYYLRRNSDGCIEHSACVNNTASE 516

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            HFMVERLI+DDLLCWAVDYKVDGFRFDLMGHIMK TMVKAK+ML  LSKD  G+DG+SIY
Sbjct: 517  HFMVERLILDDLLCWAVDYKVDGFRFDLMGHIMKRTMVKAKNMLHSLSKDTDGIDGSSIY 576

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVAKNGRG NASQFNL  TGIGSFNDRIRDA+LGGSPFGHPLQQGFVTGL 
Sbjct: 577  IYGEGWDFGEVAKNGRGVNASQFNLCGTGIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLF 636

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            LQ N +DHG +   ER LAV KDHIQVGMAANLKD+VL NC+G+EVKGSEVSTYDG PV 
Sbjct: 637  LQPNDHDHGGEEVAERMLAVSKDHIQVGMAANLKDFVLTNCEGEEVKGSEVSTYDGTPVA 696

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+  PTET+NYVSAHDNETLFDIVSLKTPM+ISV+ERCRINHLATSIIALSQGIPFFHSG
Sbjct: 697  YTVCPTETINYVSAHDNETLFDIVSLKTPMQISVEERCRINHLATSIIALSQGIPFFHSG 756

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DEMLRSKS+DRDSYNSGDWFNRLDFSY+SNNWGVGLPPKEKNE+NWPLIKPRLADPSFKP
Sbjct: 757  DEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEKNWPLIKPRLADPSFKP 816

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
            Q  HI+AAVENFLNLL+IRYSSPLFRLRTAN+IQ RVRFHNTGPSWVPG+IVM IEDGH 
Sbjct: 817  QKNHIIAAVENFLNLLKIRYSSPLFRLRTANSIQARVRFHNTGPSWVPGIIVMSIEDGHE 876

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD  YSYI+VI+N  PTE+ FASPAL+A+TL+LHPVQ+MS+D ++K STY+AS
Sbjct: 877  GVPGLSQLDPVYSYIVVIINPSPTEIKFASPALQARTLQLHPVQVMSSDGVIKNSTYEAS 936

Query: 1801 SGYFKLPPRTTAIFVEPRNV 1860
            SG F +PPRTT++FVEPR +
Sbjct: 937  SGCFIVPPRTTSVFVEPREI 956


>gb|AOQ26240.1| PUL1 [Actinidia deliciosa]
          Length = 964

 Score = 1073 bits (2776), Expect = 0.0
 Identities = 513/620 (82%), Positives = 561/620 (90%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            +D TVHP+FRGGYLAFTSQDSAG+ HLK+LS+AG+THVHLLP+F FA V DEKDKWK+ D
Sbjct: 345  TDPTVHPDFRGGYLAFTSQDSAGVRHLKRLSSAGLTHVHLLPSFHFADVADEKDKWKNAD 404

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
             QMLESLPPDS EQQ +ITAIQN+DGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKM+
Sbjct: 405  NQMLESLPPDSDEQQAHITAIQNEDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMI 464

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA N +G RVVLDVVYNHL G+GPFD+NSVLDKIVPGYYLR N +G IEHSTCVNNTASE
Sbjct: 465  QALNCVGFRVVLDVVYNHLHGNGPFDENSVLDKIVPGYYLRLNNDGCIEHSTCVNNTASE 524

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            HFMVERLI+DDLLCWAVDYKVDGFRFDLMGHIMK TMVKAK++L  LSKD  GVDG+SIY
Sbjct: 525  HFMVERLILDDLLCWAVDYKVDGFRFDLMGHIMKRTMVKAKNVLNVLSKDTRGVDGSSIY 584

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVAKNGRG NASQFN+  TGIGSFNDRIRDALLGGSPFGHPLQQGFVTGL 
Sbjct: 585  IYGEGWDFGEVAKNGRGINASQFNICGTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLL 644

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            LQ NG++HGSK +EER LA  KDHIQVGMAANL+D+VL + +GQEVKGSEV TYDG+PV 
Sbjct: 645  LQPNGHNHGSKENEERMLAGSKDHIQVGMAANLRDFVLTDFNGQEVKGSEVCTYDGLPVA 704

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            YS  PTETVNYVSAHDNETLFDIVSLKTPMEISV+ERCR+NHLATSIIALSQGIPFFHSG
Sbjct: 705  YSLSPTETVNYVSAHDNETLFDIVSLKTPMEISVEERCRLNHLATSIIALSQGIPFFHSG 764

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DEMLRSKSLDRDSYNSGDWFNRLDFSY+SNNWGVGLPPK KNE+NWPLIKP LADPS+KP
Sbjct: 765  DEMLRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKAKNEKNWPLIKPILADPSYKP 824

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
               HILAAVENFLN+L+IRYSSPLFRLRTANAIQ+RVRFHNTGPSWVPGVIVM IEDG  
Sbjct: 825  LRSHILAAVENFLNVLRIRYSSPLFRLRTANAIQQRVRFHNTGPSWVPGVIVMSIEDGQE 884

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            G+PGLSQLD  Y+YI+VIVN CP   SFA PALR +T ELHP+Q+MSTD IVK STY+A 
Sbjct: 885  GIPGLSQLDPLYAYIVVIVNACPNAASFAIPALRTRTFELHPLQVMSTDEIVKNSTYEAL 944

Query: 1801 SGYFKLPPRTTAIFVEPRNV 1860
            +G F +PPRTT++FVE RN+
Sbjct: 945  TGCFTVPPRTTSVFVESRNI 964


>gb|ONI23748.1| hypothetical protein PRUPE_2G205800 [Prunus persica]
          Length = 966

 Score = 1057 bits (2734), Expect = 0.0
 Identities = 512/620 (82%), Positives = 554/620 (89%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            SD  VHP FRGGYLAFT QDSAG  HLKKLSNAGITHVHLLPAFQFAGV+DEK+ WKSVD
Sbjct: 347  SDQAVHPEFRGGYLAFTLQDSAGAIHLKKLSNAGITHVHLLPAFQFAGVDDEKENWKSVD 406

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
             ++LE  P DS +QQ  ITAIQN DGYNWGYNPVLWGVPKGSYASN NG  R IEFRKMV
Sbjct: 407  PEVLEKFPSDSDKQQALITAIQNDDGYNWGYNPVLWGVPKGSYASNANGTYRAIEFRKMV 466

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NR GLRVVLDVVYNHL GSGP DDNSVLDKIVPGYYLRRNT+G IEHSTCVNNTASE
Sbjct: 467  QALNRFGLRVVLDVVYNHLHGSGPVDDNSVLDKIVPGYYLRRNTDGFIEHSTCVNNTASE 526

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            HFMVERLIVDDLL WAVDYKVDGFRFDLMGHIM+ TMVKAK  L  L+K++ GVDG+SIY
Sbjct: 527  HFMVERLIVDDLLHWAVDYKVDGFRFDLMGHIMRRTMVKAKDALCSLTKERDGVDGSSIY 586

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVA NGRG NASQFN+  TGIGSFNDRIRDA+LGGSPFGHPLQQGFVTGL 
Sbjct: 587  IYGEGWDFGEVANNGRGINASQFNIHGTGIGSFNDRIRDAILGGSPFGHPLQQGFVTGLL 646

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            LQ NG+DHG ++  E  LA  KDHIQVGMAANL+D+VL NC+G+EVKGSEV TY G PV 
Sbjct: 647  LQPNGHDHGPEAVAEHMLAESKDHIQVGMAANLRDFVLTNCEGKEVKGSEVLTYGGTPVA 706

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+  PTET+NYVSAHDNETLFDIVSLKTPMEISV+ERCRINHLATSIIAL+QGIPFFHSG
Sbjct: 707  YTLCPTETINYVSAHDNETLFDIVSLKTPMEISVEERCRINHLATSIIALAQGIPFFHSG 766

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DE+LRSKSLDRDSYNSGDWFNRLDF+YSSNNWGVGLPPKEKNE++WPL KPRLADPSFKP
Sbjct: 767  DEILRSKSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEKSWPLFKPRLADPSFKP 826

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
            Q  HI+AAVENF NLL+IRYSSPLFRLRTANAIQERVRFHNTGPS VPGVIVM IEDGH 
Sbjct: 827  QKSHIIAAVENFSNLLRIRYSSPLFRLRTANAIQERVRFHNTGPSLVPGVIVMSIEDGHE 886

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD  YSYI+VIVN CPTEVSFASP+L+A+TL+LHP Q+MSTD IVK+STY+AS
Sbjct: 887  GVPGLSQLDPIYSYIVVIVNACPTEVSFASPSLQARTLQLHPEQVMSTDEIVKRSTYNAS 946

Query: 1801 SGYFKLPPRTTAIFVEPRNV 1860
            +G F +PPRTT++FVEPR V
Sbjct: 947  AGCFTVPPRTTSVFVEPRGV 966


>ref|XP_007220271.1| pullulanase 1, chloroplastic [Prunus persica]
 gb|ONI23747.1| hypothetical protein PRUPE_2G205800 [Prunus persica]
          Length = 965

 Score = 1057 bits (2734), Expect = 0.0
 Identities = 512/620 (82%), Positives = 554/620 (89%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            SD  VHP FRGGYLAFT QDSAG  HLKKLSNAGITHVHLLPAFQFAGV+DEK+ WKSVD
Sbjct: 346  SDQAVHPEFRGGYLAFTLQDSAGAIHLKKLSNAGITHVHLLPAFQFAGVDDEKENWKSVD 405

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
             ++LE  P DS +QQ  ITAIQN DGYNWGYNPVLWGVPKGSYASN NG  R IEFRKMV
Sbjct: 406  PEVLEKFPSDSDKQQALITAIQNDDGYNWGYNPVLWGVPKGSYASNANGTYRAIEFRKMV 465

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NR GLRVVLDVVYNHL GSGP DDNSVLDKIVPGYYLRRNT+G IEHSTCVNNTASE
Sbjct: 466  QALNRFGLRVVLDVVYNHLHGSGPVDDNSVLDKIVPGYYLRRNTDGFIEHSTCVNNTASE 525

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            HFMVERLIVDDLL WAVDYKVDGFRFDLMGHIM+ TMVKAK  L  L+K++ GVDG+SIY
Sbjct: 526  HFMVERLIVDDLLHWAVDYKVDGFRFDLMGHIMRRTMVKAKDALCSLTKERDGVDGSSIY 585

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVA NGRG NASQFN+  TGIGSFNDRIRDA+LGGSPFGHPLQQGFVTGL 
Sbjct: 586  IYGEGWDFGEVANNGRGINASQFNIHGTGIGSFNDRIRDAILGGSPFGHPLQQGFVTGLL 645

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            LQ NG+DHG ++  E  LA  KDHIQVGMAANL+D+VL NC+G+EVKGSEV TY G PV 
Sbjct: 646  LQPNGHDHGPEAVAEHMLAESKDHIQVGMAANLRDFVLTNCEGKEVKGSEVLTYGGTPVA 705

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+  PTET+NYVSAHDNETLFDIVSLKTPMEISV+ERCRINHLATSIIAL+QGIPFFHSG
Sbjct: 706  YTLCPTETINYVSAHDNETLFDIVSLKTPMEISVEERCRINHLATSIIALAQGIPFFHSG 765

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DE+LRSKSLDRDSYNSGDWFNRLDF+YSSNNWGVGLPPKEKNE++WPL KPRLADPSFKP
Sbjct: 766  DEILRSKSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEKSWPLFKPRLADPSFKP 825

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
            Q  HI+AAVENF NLL+IRYSSPLFRLRTANAIQERVRFHNTGPS VPGVIVM IEDGH 
Sbjct: 826  QKSHIIAAVENFSNLLRIRYSSPLFRLRTANAIQERVRFHNTGPSLVPGVIVMSIEDGHE 885

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD  YSYI+VIVN CPTEVSFASP+L+A+TL+LHP Q+MSTD IVK+STY+AS
Sbjct: 886  GVPGLSQLDPIYSYIVVIVNACPTEVSFASPSLQARTLQLHPEQVMSTDEIVKRSTYNAS 945

Query: 1801 SGYFKLPPRTTAIFVEPRNV 1860
            +G F +PPRTT++FVEPR V
Sbjct: 946  AGCFTVPPRTTSVFVEPRGV 965


>ref|XP_023879674.1| pullulanase 1, chloroplastic isoform X2 [Quercus suber]
 gb|POE76574.1| pullulanase 1, chloroplastic [Quercus suber]
          Length = 961

 Score = 1057 bits (2733), Expect = 0.0
 Identities = 506/618 (81%), Positives = 558/618 (90%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            +DYTVHP FRGGYLAFT QDS+G+ HLKKLSNAG+THVHLLP FQFAGV+DEK+ WK VD
Sbjct: 344  NDYTVHPEFRGGYLAFTLQDSSGVLHLKKLSNAGLTHVHLLPTFQFAGVDDEKENWKCVD 403

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
            T MLE LPPDSV+QQ+ ITAIQ+ DGYNWGYNPVLWGVPKGSYASNPNGP R IEFRKM+
Sbjct: 404  TNMLEKLPPDSVQQQDLITAIQDNDGYNWGYNPVLWGVPKGSYASNPNGPYRIIEFRKMI 463

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NRIGLR+VLDVVYNH+ GSGPFD+NSVLDKIVPGYYLR+NT+G IE+STCVNNTASE
Sbjct: 464  QALNRIGLRIVLDVVYNHVHGSGPFDENSVLDKIVPGYYLRKNTDGLIENSTCVNNTASE 523

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            H MVERLI+DDLL WAV YKVDGFRFDLMGHIMK TMVKAK  L  L+K++ G+DG+SIY
Sbjct: 524  HVMVERLILDDLLHWAVHYKVDGFRFDLMGHIMKSTMVKAKDALHSLTKERDGIDGSSIY 583

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVAKNGRG NASQFNLS T IGSFNDRIRDA+LGGSPFGHPLQQGFVTGL 
Sbjct: 584  IYGEGWDFGEVAKNGRGVNASQFNLSGTQIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLF 643

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            LQ NG+DHG++++ E  LAV KDHIQVGMAANL+D+VL N +G+EVKGSEV TY G PV 
Sbjct: 644  LQPNGHDHGTETNAECMLAVAKDHIQVGMAANLRDFVLTNFEGKEVKGSEVLTYGGAPVA 703

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+  PTETVNYVSAHDNETLFDIVSLKTP EISVD+RCRINHLATS+IALSQGIPFFHSG
Sbjct: 704  YALSPTETVNYVSAHDNETLFDIVSLKTPTEISVDKRCRINHLATSVIALSQGIPFFHSG 763

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DEMLRSKSLDRDSYNSGDWFNRLDF+Y+SNNWGVGLPPKEKNE+NWPLIKPRLADPSFKP
Sbjct: 764  DEMLRSKSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPKEKNEKNWPLIKPRLADPSFKP 823

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
            Q  HILAAV+NFLNLL IRYSSPLFRL+TANAIQERVRFHNTGPS +PG IVM IEDG+ 
Sbjct: 824  QKIHILAAVDNFLNLLCIRYSSPLFRLKTANAIQERVRFHNTGPSLIPGTIVMSIEDGYE 883

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD  YSYI+VIVN  PTEVSFA PALRA+TL+LHP+Q+MS D +VK STY+ S
Sbjct: 884  GVPGLSQLDPIYSYIVVIVNASPTEVSFACPALRARTLKLHPIQVMSIDELVKSSTYEES 943

Query: 1801 SGYFKLPPRTTAIFVEPR 1854
            SG F +PPRTT++FVEPR
Sbjct: 944  SGCFTVPPRTTSVFVEPR 961


>ref|XP_023879673.1| pullulanase 1, chloroplastic isoform X1 [Quercus suber]
 gb|POE76573.1| pullulanase 1, chloroplastic [Quercus suber]
          Length = 963

 Score = 1057 bits (2733), Expect = 0.0
 Identities = 506/618 (81%), Positives = 558/618 (90%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            +DYTVHP FRGGYLAFT QDS+G+ HLKKLSNAG+THVHLLP FQFAGV+DEK+ WK VD
Sbjct: 346  NDYTVHPEFRGGYLAFTLQDSSGVLHLKKLSNAGLTHVHLLPTFQFAGVDDEKENWKCVD 405

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
            T MLE LPPDSV+QQ+ ITAIQ+ DGYNWGYNPVLWGVPKGSYASNPNGP R IEFRKM+
Sbjct: 406  TNMLEKLPPDSVQQQDLITAIQDNDGYNWGYNPVLWGVPKGSYASNPNGPYRIIEFRKMI 465

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NRIGLR+VLDVVYNH+ GSGPFD+NSVLDKIVPGYYLR+NT+G IE+STCVNNTASE
Sbjct: 466  QALNRIGLRIVLDVVYNHVHGSGPFDENSVLDKIVPGYYLRKNTDGLIENSTCVNNTASE 525

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            H MVERLI+DDLL WAV YKVDGFRFDLMGHIMK TMVKAK  L  L+K++ G+DG+SIY
Sbjct: 526  HVMVERLILDDLLHWAVHYKVDGFRFDLMGHIMKSTMVKAKDALHSLTKERDGIDGSSIY 585

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVAKNGRG NASQFNLS T IGSFNDRIRDA+LGGSPFGHPLQQGFVTGL 
Sbjct: 586  IYGEGWDFGEVAKNGRGVNASQFNLSGTQIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLF 645

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            LQ NG+DHG++++ E  LAV KDHIQVGMAANL+D+VL N +G+EVKGSEV TY G PV 
Sbjct: 646  LQPNGHDHGTETNAECMLAVAKDHIQVGMAANLRDFVLTNFEGKEVKGSEVLTYGGAPVA 705

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+  PTETVNYVSAHDNETLFDIVSLKTP EISVD+RCRINHLATS+IALSQGIPFFHSG
Sbjct: 706  YALSPTETVNYVSAHDNETLFDIVSLKTPTEISVDKRCRINHLATSVIALSQGIPFFHSG 765

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DEMLRSKSLDRDSYNSGDWFNRLDF+Y+SNNWGVGLPPKEKNE+NWPLIKPRLADPSFKP
Sbjct: 766  DEMLRSKSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPKEKNEKNWPLIKPRLADPSFKP 825

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
            Q  HILAAV+NFLNLL IRYSSPLFRL+TANAIQERVRFHNTGPS +PG IVM IEDG+ 
Sbjct: 826  QKIHILAAVDNFLNLLCIRYSSPLFRLKTANAIQERVRFHNTGPSLIPGTIVMSIEDGYE 885

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD  YSYI+VIVN  PTEVSFA PALRA+TL+LHP+Q+MS D +VK STY+ S
Sbjct: 886  GVPGLSQLDPIYSYIVVIVNASPTEVSFACPALRARTLKLHPIQVMSIDELVKSSTYEES 945

Query: 1801 SGYFKLPPRTTAIFVEPR 1854
            SG F +PPRTT++FVEPR
Sbjct: 946  SGCFTVPPRTTSVFVEPR 963


>ref|XP_009587792.1| PREDICTED: pullulanase 1, chloroplastic isoform X4 [Nicotiana
            tomentosiformis]
          Length = 870

 Score = 1051 bits (2717), Expect = 0.0
 Identities = 505/618 (81%), Positives = 552/618 (89%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            +D TV   F+GGYLAFT QDSAG+ HLK+LS+AGITHVHLLP FQFAGVEDEK KWK VD
Sbjct: 253  NDPTVPHEFQGGYLAFTLQDSAGVKHLKRLSSAGITHVHLLPTFQFAGVEDEKHKWKHVD 312

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
             + L S PPDS EQQ  ITAIQN+DGYNWGYNPVLWGVPKGSYASN NGPCR IEFRKMV
Sbjct: 313  IEKLNSFPPDSEEQQALITAIQNEDGYNWGYNPVLWGVPKGSYASNANGPCRIIEFRKMV 372

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NRIGLRVVLDVVYNH+  SG FDD SVLDKIVPGYYLRRN +G IEHSTCVNNTASE
Sbjct: 373  QALNRIGLRVVLDVVYNHINASGHFDDFSVLDKIVPGYYLRRNADGGIEHSTCVNNTASE 432

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            HFMVERLI+DDL CWAV YK+DGFRFDLMGHIMKHTMVKAKS+L  LSKDK+GVDG SIY
Sbjct: 433  HFMVERLILDDLKCWAVHYKIDGFRFDLMGHIMKHTMVKAKSLLNSLSKDKNGVDGPSIY 492

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVA NGRG NASQFNL  +GIGSFNDRIRDALLGGSPFGHPL QGFVTGL 
Sbjct: 493  IYGEGWDFGEVANNGRGINASQFNLFGSGIGSFNDRIRDALLGGSPFGHPLHQGFVTGLY 552

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            L+ NG+D G K++ ER LAV KDHIQVGMAANLKD+VL NCDG+EVKGSEV  +D  PVG
Sbjct: 553  LEPNGHDLGDKANVERMLAVSKDHIQVGMAANLKDFVLSNCDGEEVKGSEVLLHDRKPVG 612

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+S P ETVNYVSAHDNETLFDI+SLKTP +ISV+ERCR+NHLATS+IALSQGIPFFHSG
Sbjct: 613  YASSPIETVNYVSAHDNETLFDIISLKTPKDISVEERCRMNHLATSVIALSQGIPFFHSG 672

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DEMLRSKS+DRDSYNSGDWFNRLDFSY+SNNWGVGLPPKEKNE NWPLIKPRLADPS+KP
Sbjct: 673  DEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPLIKPRLADPSYKP 732

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
            Q  HILAAVENF NL+QIRYSSPLFRLRTAN+IQERVRFHNT PSW+PG+IVM IEDGH 
Sbjct: 733  QKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQERVRFHNTCPSWIPGLIVMSIEDGHQ 792

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD  YSYI+VI+N  PT+VSFA+PALRA++L+LHP+QM STD++VK STYDAS
Sbjct: 793  GVPGLSQLDPIYSYIVVIINPGPTDVSFANPALRARSLQLHPIQMNSTDDVVKNSTYDAS 852

Query: 1801 SGYFKLPPRTTAIFVEPR 1854
            SG F +P RTT++FVEPR
Sbjct: 853  SGCFNVPTRTTSVFVEPR 870


>ref|XP_009587786.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Nicotiana
            tomentosiformis]
          Length = 952

 Score = 1051 bits (2717), Expect = 0.0
 Identities = 505/618 (81%), Positives = 552/618 (89%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            +D TV   F+GGYLAFT QDSAG+ HLK+LS+AGITHVHLLP FQFAGVEDEK KWK VD
Sbjct: 335  NDPTVPHEFQGGYLAFTLQDSAGVKHLKRLSSAGITHVHLLPTFQFAGVEDEKHKWKHVD 394

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
             + L S PPDS EQQ  ITAIQN+DGYNWGYNPVLWGVPKGSYASN NGPCR IEFRKMV
Sbjct: 395  IEKLNSFPPDSEEQQALITAIQNEDGYNWGYNPVLWGVPKGSYASNANGPCRIIEFRKMV 454

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NRIGLRVVLDVVYNH+  SG FDD SVLDKIVPGYYLRRN +G IEHSTCVNNTASE
Sbjct: 455  QALNRIGLRVVLDVVYNHINASGHFDDFSVLDKIVPGYYLRRNADGGIEHSTCVNNTASE 514

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            HFMVERLI+DDL CWAV YK+DGFRFDLMGHIMKHTMVKAKS+L  LSKDK+GVDG SIY
Sbjct: 515  HFMVERLILDDLKCWAVHYKIDGFRFDLMGHIMKHTMVKAKSLLNSLSKDKNGVDGPSIY 574

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVA NGRG NASQFNL  +GIGSFNDRIRDALLGGSPFGHPL QGFVTGL 
Sbjct: 575  IYGEGWDFGEVANNGRGINASQFNLFGSGIGSFNDRIRDALLGGSPFGHPLHQGFVTGLY 634

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            L+ NG+D G K++ ER LAV KDHIQVGMAANLKD+VL NCDG+EVKGSEV  +D  PVG
Sbjct: 635  LEPNGHDLGDKANVERMLAVSKDHIQVGMAANLKDFVLSNCDGEEVKGSEVLLHDRKPVG 694

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+S P ETVNYVSAHDNETLFDI+SLKTP +ISV+ERCR+NHLATS+IALSQGIPFFHSG
Sbjct: 695  YASSPIETVNYVSAHDNETLFDIISLKTPKDISVEERCRMNHLATSVIALSQGIPFFHSG 754

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DEMLRSKS+DRDSYNSGDWFNRLDFSY+SNNWGVGLPPKEKNE NWPLIKPRLADPS+KP
Sbjct: 755  DEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPLIKPRLADPSYKP 814

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
            Q  HILAAVENF NL+QIRYSSPLFRLRTAN+IQERVRFHNT PSW+PG+IVM IEDGH 
Sbjct: 815  QKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQERVRFHNTCPSWIPGLIVMSIEDGHQ 874

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD  YSYI+VI+N  PT+VSFA+PALRA++L+LHP+QM STD++VK STYDAS
Sbjct: 875  GVPGLSQLDPIYSYIVVIINPGPTDVSFANPALRARSLQLHPIQMNSTDDVVKNSTYDAS 934

Query: 1801 SGYFKLPPRTTAIFVEPR 1854
            SG F +P RTT++FVEPR
Sbjct: 935  SGCFNVPTRTTSVFVEPR 952


>ref|XP_016443816.1| PREDICTED: pullulanase 1, chloroplastic-like isoform X1 [Nicotiana
            tabacum]
          Length = 952

 Score = 1048 bits (2711), Expect = 0.0
 Identities = 503/618 (81%), Positives = 552/618 (89%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            +D TV   F+GGYLAFT QDSAG+ HLK+LS+AGITHVHLLP FQFAGVEDEK KWK VD
Sbjct: 335  NDPTVPHEFQGGYLAFTLQDSAGVKHLKRLSSAGITHVHLLPTFQFAGVEDEKHKWKHVD 394

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
             + L S PPDS EQQ  ITAIQN+DGYNWGYNPV+WGVPKGSYASN NGPCR IEFRKMV
Sbjct: 395  IEKLNSFPPDSEEQQALITAIQNEDGYNWGYNPVIWGVPKGSYASNANGPCRIIEFRKMV 454

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NRIGLRVVLDVVYNH+  SG FDD SVLDKIVPGYYLRRN +G IEHSTCVNNTASE
Sbjct: 455  QALNRIGLRVVLDVVYNHINASGHFDDFSVLDKIVPGYYLRRNADGGIEHSTCVNNTASE 514

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            HFMVERLI+DDL CWAV +K+DGFRFDLMGHIMK TMVKAKS+L  LSKDK+GVDG SIY
Sbjct: 515  HFMVERLILDDLKCWAVHFKIDGFRFDLMGHIMKRTMVKAKSLLNSLSKDKNGVDGPSIY 574

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVA NGRG NASQFNL  +GIGSFNDRIRDALLGGSPFGHPL QGFVTGL 
Sbjct: 575  IYGEGWDFGEVANNGRGINASQFNLFGSGIGSFNDRIRDALLGGSPFGHPLHQGFVTGLY 634

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            L+ NG+D G K++ ER LAV KDHIQVGMAANLKD+VL NCDG+EVKGSEV  +D  PVG
Sbjct: 635  LEPNGHDLGDKANVERMLAVSKDHIQVGMAANLKDFVLTNCDGEEVKGSEVFLHDRKPVG 694

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+S P ETVNYVSAHDNETLFDI+SLKTP +ISV+ERCR+NHLATS+IALSQGIPFFHSG
Sbjct: 695  YASSPIETVNYVSAHDNETLFDIISLKTPKDISVEERCRMNHLATSVIALSQGIPFFHSG 754

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DEMLRSKS+DRDSYNSGDWFNRLDFSY+SNNWGVGLPPKEKNE NWPLIKPRLADPS+KP
Sbjct: 755  DEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPLIKPRLADPSYKP 814

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
            Q  HILAAVENF NL+QIRYSSPLFRLRTAN+IQERVRFHNTGPSW+PG+IVM IEDGH 
Sbjct: 815  QKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQERVRFHNTGPSWIPGLIVMSIEDGHP 874

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD  YSYI+VI+N  PT+VSFA+PALRA++L+LHP+QM STD++VK STYDAS
Sbjct: 875  GVPGLSQLDPIYSYIVVIINPDPTDVSFANPALRARSLQLHPIQMNSTDDVVKNSTYDAS 934

Query: 1801 SGYFKLPPRTTAIFVEPR 1854
            SG F +P RTT++FVEPR
Sbjct: 935  SGCFNVPARTTSVFVEPR 952


>ref|XP_009791833.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Nicotiana
            sylvestris]
          Length = 952

 Score = 1048 bits (2711), Expect = 0.0
 Identities = 503/618 (81%), Positives = 552/618 (89%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            +D TV   F+GGYLAFT QDSAG+ HLK+LS+AGITHVHLLP FQFAGVEDEK KWK VD
Sbjct: 335  NDPTVPHEFQGGYLAFTLQDSAGVKHLKRLSSAGITHVHLLPTFQFAGVEDEKHKWKHVD 394

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
             + L S PPDS EQQ  ITAIQN+DGYNWGYNPV+WGVPKGSYASN NGPCR IEFRKMV
Sbjct: 395  IEKLNSFPPDSEEQQALITAIQNEDGYNWGYNPVIWGVPKGSYASNANGPCRIIEFRKMV 454

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NRIGLRVVLDVVYNH+  SG FDD SVLDKIVPGYYLRRN +G IEHSTCVNNTASE
Sbjct: 455  QALNRIGLRVVLDVVYNHINASGHFDDFSVLDKIVPGYYLRRNADGGIEHSTCVNNTASE 514

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            HFMVERLI+DDL CWAV +K+DGFRFDLMGHIMK TMVKAKS+L  LSKDK+GVDG SIY
Sbjct: 515  HFMVERLILDDLKCWAVHFKIDGFRFDLMGHIMKRTMVKAKSLLNSLSKDKNGVDGPSIY 574

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVA NGRG NASQFNL  +GIGSFNDRIRDALLGGSPFGHPL QGFVTGL 
Sbjct: 575  IYGEGWDFGEVANNGRGINASQFNLFGSGIGSFNDRIRDALLGGSPFGHPLHQGFVTGLY 634

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            L+ NG+D G K++ ER LAV KDHIQVGMAANLKD+VL NCDG+EVKGSEV  +D  PVG
Sbjct: 635  LEPNGHDLGDKANVERMLAVSKDHIQVGMAANLKDFVLTNCDGEEVKGSEVFLHDRKPVG 694

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+S P ETVNYVSAHDNETLFDI+SLKTP +ISV+ERCR+NHLATS+IALSQGIPFFHSG
Sbjct: 695  YASSPIETVNYVSAHDNETLFDIISLKTPKDISVEERCRMNHLATSVIALSQGIPFFHSG 754

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DEMLRSKS+DRDSYNSGDWFNRLDFSY+SNNWGVGLPPKEKNE NWPLIKPRLADPS+KP
Sbjct: 755  DEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPLIKPRLADPSYKP 814

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
            Q  HILAAVENF NL+QIRYSSPLFRLRTAN+IQERVRFHNTGPSW+PG+IVM IEDGH 
Sbjct: 815  QKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQERVRFHNTGPSWIPGLIVMSIEDGHP 874

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD  YSYI+VI+N  PT+VSFA+PALRA++L+LHP+QM STD++VK STYDAS
Sbjct: 875  GVPGLSQLDPIYSYIVVIINPDPTDVSFANPALRARSLQLHPIQMNSTDDVVKNSTYDAS 934

Query: 1801 SGYFKLPPRTTAIFVEPR 1854
            SG F +P RTT++FVEPR
Sbjct: 935  SGCFNVPARTTSVFVEPR 952


>ref|XP_018808475.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Juglans regia]
          Length = 969

 Score = 1048 bits (2710), Expect = 0.0
 Identities = 503/620 (81%), Positives = 552/620 (89%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            +D+TV P FRG YLAFT Q S+G+ HLKKLS+AGITHVHLLP FQFAGV+DEK+ WK VD
Sbjct: 350  NDHTVPPEFRGCYLAFTLQGSSGVLHLKKLSDAGITHVHLLPTFQFAGVDDEKENWKCVD 409

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
            T++LE LPPDS EQQ  ITAIQN DGYNWGYNPVLWGVPKGSYASNPNGPCR IEFRKM+
Sbjct: 410  TKILEQLPPDSDEQQALITAIQNDDGYNWGYNPVLWGVPKGSYASNPNGPCRIIEFRKMI 469

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNT+G IEHSTC+NNTASE
Sbjct: 470  QALNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTDGFIEHSTCMNNTASE 529

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            H MVERLI+DDLL WA+ +KVDGFRFDLMGHIMK TMVKAK  L  L+K++ G+DG+SIY
Sbjct: 530  HVMVERLILDDLLHWAIHHKVDGFRFDLMGHIMKSTMVKAKDALHSLTKERDGIDGSSIY 589

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVAKNGRG NASQFNL  TGIGSFNDRIRDA+LGGSPFGHPLQQGF TGL 
Sbjct: 590  IYGEGWDFGEVAKNGRGVNASQFNLGGTGIGSFNDRIRDAILGGSPFGHPLQQGFATGLL 649

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            LQ NG+D G+++  E  LAV KDHIQVGMAANL+D+V+ +  G+EVKGSEV T+DG PV 
Sbjct: 650  LQPNGHDQGTEAVSECMLAVAKDHIQVGMAANLRDFVITSYQGEEVKGSEVLTHDGTPVA 709

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+   TETVNYVSAHDNETLFDIVSLKTP EISVDERCRINHLAT +IALSQGIPFFHSG
Sbjct: 710  YALCSTETVNYVSAHDNETLFDIVSLKTPKEISVDERCRINHLATGVIALSQGIPFFHSG 769

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DE+LRSKSLDRDSYNSGDWFNRLDF+Y+SNNWGVGLPPKEKNE+NWPLIKPRLADPSFKP
Sbjct: 770  DEILRSKSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPKEKNEKNWPLIKPRLADPSFKP 829

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
            Q  HILAAV+NFLNLL+IRYSSPLFRLRTANAIQERV FHNTGPS  PGVIVM I+DGH 
Sbjct: 830  QRVHILAAVDNFLNLLRIRYSSPLFRLRTANAIQERVHFHNTGPSGAPGVIVMSIQDGHE 889

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD TYSYI+VI+N CPT+VSFA PALR +TL+LHPVQ MSTD +VK STY+AS
Sbjct: 890  GVPGLSQLDPTYSYIVVIINACPTKVSFACPALRTRTLKLHPVQAMSTDELVKSSTYEAS 949

Query: 1801 SGYFKLPPRTTAIFVEPRNV 1860
            SG   +PPRTT +FVEPR V
Sbjct: 950  SGCLAVPPRTTVVFVEPREV 969


>ref|XP_018808474.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Juglans regia]
          Length = 970

 Score = 1048 bits (2710), Expect = 0.0
 Identities = 503/620 (81%), Positives = 552/620 (89%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            +D+TV P FRG YLAFT Q S+G+ HLKKLS+AGITHVHLLP FQFAGV+DEK+ WK VD
Sbjct: 351  NDHTVPPEFRGCYLAFTLQGSSGVLHLKKLSDAGITHVHLLPTFQFAGVDDEKENWKCVD 410

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
            T++LE LPPDS EQQ  ITAIQN DGYNWGYNPVLWGVPKGSYASNPNGPCR IEFRKM+
Sbjct: 411  TKILEQLPPDSDEQQALITAIQNDDGYNWGYNPVLWGVPKGSYASNPNGPCRIIEFRKMI 470

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNT+G IEHSTC+NNTASE
Sbjct: 471  QALNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTDGFIEHSTCMNNTASE 530

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            H MVERLI+DDLL WA+ +KVDGFRFDLMGHIMK TMVKAK  L  L+K++ G+DG+SIY
Sbjct: 531  HVMVERLILDDLLHWAIHHKVDGFRFDLMGHIMKSTMVKAKDALHSLTKERDGIDGSSIY 590

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVAKNGRG NASQFNL  TGIGSFNDRIRDA+LGGSPFGHPLQQGF TGL 
Sbjct: 591  IYGEGWDFGEVAKNGRGVNASQFNLGGTGIGSFNDRIRDAILGGSPFGHPLQQGFATGLL 650

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            LQ NG+D G+++  E  LAV KDHIQVGMAANL+D+V+ +  G+EVKGSEV T+DG PV 
Sbjct: 651  LQPNGHDQGTEAVSECMLAVAKDHIQVGMAANLRDFVITSYQGEEVKGSEVLTHDGTPVA 710

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+   TETVNYVSAHDNETLFDIVSLKTP EISVDERCRINHLAT +IALSQGIPFFHSG
Sbjct: 711  YALCSTETVNYVSAHDNETLFDIVSLKTPKEISVDERCRINHLATGVIALSQGIPFFHSG 770

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DE+LRSKSLDRDSYNSGDWFNRLDF+Y+SNNWGVGLPPKEKNE+NWPLIKPRLADPSFKP
Sbjct: 771  DEILRSKSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPKEKNEKNWPLIKPRLADPSFKP 830

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
            Q  HILAAV+NFLNLL+IRYSSPLFRLRTANAIQERV FHNTGPS  PGVIVM I+DGH 
Sbjct: 831  QRVHILAAVDNFLNLLRIRYSSPLFRLRTANAIQERVHFHNTGPSGAPGVIVMSIQDGHE 890

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD TYSYI+VI+N CPT+VSFA PALR +TL+LHPVQ MSTD +VK STY+AS
Sbjct: 891  GVPGLSQLDPTYSYIVVIINACPTKVSFACPALRTRTLKLHPVQAMSTDELVKSSTYEAS 950

Query: 1801 SGYFKLPPRTTAIFVEPRNV 1860
            SG   +PPRTT +FVEPR V
Sbjct: 951  SGCLAVPPRTTVVFVEPREV 970


>ref|XP_016436612.1| PREDICTED: pullulanase 1, chloroplastic-like [Nicotiana tabacum]
          Length = 952

 Score = 1048 bits (2709), Expect = 0.0
 Identities = 504/618 (81%), Positives = 551/618 (89%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            +D TV   F+GGYLAFT QDSAG+ HLK+LS+AGITHVHLLP FQFAGVEDEK KWK VD
Sbjct: 335  NDPTVPHEFQGGYLAFTLQDSAGVKHLKRLSSAGITHVHLLPTFQFAGVEDEKHKWKHVD 394

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
             + L S PPDS EQQ  ITAIQN+DGYNWGYNPVLWGVPKGSYASN NGPCR IEFRKMV
Sbjct: 395  IEKLNSFPPDSEEQQALITAIQNKDGYNWGYNPVLWGVPKGSYASNANGPCRIIEFRKMV 454

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NRIGLRVVLDVVYNH+  SG FDD SVLDKIVPGYYLRRN +G IEHSTCVNNTASE
Sbjct: 455  QALNRIGLRVVLDVVYNHINASGHFDDFSVLDKIVPGYYLRRNADGGIEHSTCVNNTASE 514

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            HFMVERLI+DDL CWAV YK+DGFRFDLMGHIMK TMVKAKS+L  LSKDK+GVDG SIY
Sbjct: 515  HFMVERLILDDLKCWAVHYKIDGFRFDLMGHIMKRTMVKAKSLLNSLSKDKNGVDGPSIY 574

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVA NGRG NASQFNL  +GIGSFNDRIRDALLGGSPFGHPL QGFVTGL 
Sbjct: 575  IYGEGWDFGEVANNGRGINASQFNLFGSGIGSFNDRIRDALLGGSPFGHPLHQGFVTGLY 634

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            L+ NG+D G K++ ER LAV KDHIQVGMAANLKD+VL NCDG+EVKGSEV  +D  PVG
Sbjct: 635  LEPNGHDLGDKANVERMLAVSKDHIQVGMAANLKDFVLSNCDGEEVKGSEVLLHDRKPVG 694

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+S P ETVNYVSAHDNETLFDI+SLKTP +ISV+ERCR+NHLATS+IALSQGIPFFHSG
Sbjct: 695  YASSPIETVNYVSAHDNETLFDIISLKTPKDISVEERCRMNHLATSVIALSQGIPFFHSG 754

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DEMLRSKS+DRDSYNSGDWFNRLDFSY+SNNWG GLPPKEKNE NWPLIKPRLADPS+KP
Sbjct: 755  DEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGGGLPPKEKNEWNWPLIKPRLADPSYKP 814

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
            Q  HILAAVENF NL+QIRYSSPLFRLRTAN+IQERVRFHNTGPSW+PG+IVM IEDGH 
Sbjct: 815  QKSHILAAVENFSNLVQIRYSSPLFRLRTANSIQERVRFHNTGPSWIPGLIVMSIEDGHQ 874

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD  YSYI+VI+N  PT+VSFA+PALRA++L+LHP+QM STD++VK STYDAS
Sbjct: 875  GVPGLSQLDPIYSYIVVIINPGPTDVSFANPALRARSLQLHPIQMNSTDDVVKNSTYDAS 934

Query: 1801 SGYFKLPPRTTAIFVEPR 1854
            SG F +P RTT++FVEPR
Sbjct: 935  SGCFNVPTRTTSVFVEPR 952


>ref|XP_021690508.1| pullulanase 1, chloroplastic isoform X1 [Hevea brasiliensis]
          Length = 965

 Score = 1046 bits (2704), Expect = 0.0
 Identities = 496/620 (80%), Positives = 553/620 (89%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            +D+TV P+ RGGYLAFT +DSA + HLKKLSNAGITHVHLLP FQFAGV+D ++ WKSVD
Sbjct: 346  NDHTVRPDLRGGYLAFTLEDSASVLHLKKLSNAGITHVHLLPTFQFAGVDDVRENWKSVD 405

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
              MLE LPPDSVEQQ +ITAIQN DGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV
Sbjct: 406  NNMLEKLPPDSVEQQAHITAIQNDDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 465

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA N IGLRVVLDVVYNHL GSGPFD+NSVLDK+VPGYYLRRN +G IE+STCVNNTASE
Sbjct: 466  QALNHIGLRVVLDVVYNHLHGSGPFDENSVLDKVVPGYYLRRNNDGFIENSTCVNNTASE 525

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            H+MVERLI+DDLL WAV+YKVDGFRFDLMGHIMK TM+KAK  L  L+ +++GVDG+SIY
Sbjct: 526  HYMVERLIIDDLLSWAVNYKVDGFRFDLMGHIMKSTMMKAKDALLSLTNERNGVDGSSIY 585

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVAKNGRG NASQFNL  TGIGSFNDRIRDA+LGGSPFGHPLQQGFVTGL 
Sbjct: 586  IYGEGWDFGEVAKNGRGINASQFNLGGTGIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLM 645

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            LQ NG+DHG K+ EE  L + KDHIQ GMAANL+D+VL+N +G+EVKGSE+    G PV 
Sbjct: 646  LQPNGHDHGGKAVEELMLTIAKDHIQAGMAANLRDFVLVNSEGKEVKGSEILMNGGAPVA 705

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+  P ET+NYVSAHDNETLFDIV +KTPM+ISVDERCR+NHLATSIIA+SQGIPFFH+G
Sbjct: 706  YALCPIETINYVSAHDNETLFDIVCMKTPMKISVDERCRLNHLATSIIAMSQGIPFFHAG 765

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DEMLRSKSLDRDSYNSGDWFNRLDFSY+S+NWGVGLPPKEKNE++WPLIKPRLAD SFKP
Sbjct: 766  DEMLRSKSLDRDSYNSGDWFNRLDFSYNSSNWGVGLPPKEKNERHWPLIKPRLADESFKP 825

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
            Q  HILAAVENFL++LQIRYSSPLFRL TANAIQERVRFHNTGPSWVPGVIVM IEDGH 
Sbjct: 826  QKHHILAAVENFLDVLQIRYSSPLFRLTTANAIQERVRFHNTGPSWVPGVIVMSIEDGHE 885

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            G+PGLSQLD  YSYI+VI N CPT+VSFASPALR +  +LHPVQ MS+D +VKKSTY+AS
Sbjct: 886  GLPGLSQLDPIYSYIVVIFNTCPTKVSFASPALRPRYFQLHPVQAMSSDEVVKKSTYEAS 945

Query: 1801 SGYFKLPPRTTAIFVEPRNV 1860
            SG F +PP TT++FVEPR +
Sbjct: 946  SGCFTVPPMTTSVFVEPRKI 965


>ref|XP_019236140.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Nicotiana
            attenuata]
          Length = 957

 Score = 1045 bits (2702), Expect = 0.0
 Identities = 502/618 (81%), Positives = 551/618 (89%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            +D TV   F+GGYLAFT QDSAG+ HLK+LS+AGITHVHLLP FQFAGVEDEK KWK VD
Sbjct: 340  NDPTVPHEFQGGYLAFTLQDSAGVKHLKRLSSAGITHVHLLPTFQFAGVEDEKHKWKHVD 399

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
             + L S PPDS EQQ  ITAIQN+DGYNWGYNPVLWGVPKGSYASN NGPCR IEFRKMV
Sbjct: 400  IEKLNSFPPDSEEQQALITAIQNEDGYNWGYNPVLWGVPKGSYASNANGPCRIIEFRKMV 459

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NRIGLRVV DVVYNH+  SG FDD SVLDKIVPGYYLRRN +G IE+STCVNNTASE
Sbjct: 460  QALNRIGLRVVSDVVYNHINASGHFDDFSVLDKIVPGYYLRRNVDGGIENSTCVNNTASE 519

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            HFMVERLI+DDL CWAV YK+DGFRFDLMGHIMK TMVKAKS+L  LSKDK+GVDG SIY
Sbjct: 520  HFMVERLILDDLKCWAVHYKIDGFRFDLMGHIMKRTMVKAKSLLNSLSKDKNGVDGPSIY 579

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVA +GRG NASQFNL  +GIGSFNDRIRDALLGGSPFGHPL QGFVTGL 
Sbjct: 580  IYGEGWDFGEVANSGRGINASQFNLFGSGIGSFNDRIRDALLGGSPFGHPLHQGFVTGLY 639

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            L+ NG+D G K++ ER LAV KDHIQVGMAANLKD+VL NCDG+EVKGSEV  +D  PVG
Sbjct: 640  LEPNGHDLGDKANVERMLAVSKDHIQVGMAANLKDFVLTNCDGEEVKGSEVLLHDRKPVG 699

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+S P ETVNYVSAHDNETLFDI+SLKTP +ISV+ERCR+NHLATS+IALSQGIPFFHSG
Sbjct: 700  YASSPVETVNYVSAHDNETLFDIISLKTPKDISVEERCRMNHLATSVIALSQGIPFFHSG 759

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DEMLRSKS+DRDSYNSGDWFNRLDFSY+SNNWGVGLPPKEKNE NWPLIKPRLADPS+KP
Sbjct: 760  DEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPLIKPRLADPSYKP 819

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
            Q  HILAAVENF NL+QIRYSSPLFRLRTAN+IQERVRFHNTGPSW+PG+IVM IEDGH 
Sbjct: 820  QKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQERVRFHNTGPSWIPGLIVMSIEDGHQ 879

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD  YSYI+VI+N  PT+VSFA+PALRA++L+LHP+QM STD++VK STYDAS
Sbjct: 880  GVPGLSQLDPIYSYIVVIINPGPTDVSFANPALRARSLQLHPIQMNSTDDVVKNSTYDAS 939

Query: 1801 SGYFKLPPRTTAIFVEPR 1854
            SG F +P RTT++FVEPR
Sbjct: 940  SGCFNVPARTTSVFVEPR 957


>ref|XP_019236141.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Nicotiana
            attenuata]
 gb|OIT23986.1| pullulanase 1, chloroplastic [Nicotiana attenuata]
          Length = 955

 Score = 1045 bits (2702), Expect = 0.0
 Identities = 502/618 (81%), Positives = 551/618 (89%)
 Frame = +1

Query: 1    SDYTVHPNFRGGYLAFTSQDSAGINHLKKLSNAGITHVHLLPAFQFAGVEDEKDKWKSVD 180
            +D TV   F+GGYLAFT QDSAG+ HLK+LS+AGITHVHLLP FQFAGVEDEK KWK VD
Sbjct: 338  NDPTVPHEFQGGYLAFTLQDSAGVKHLKRLSSAGITHVHLLPTFQFAGVEDEKHKWKHVD 397

Query: 181  TQMLESLPPDSVEQQEYITAIQNQDGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMV 360
             + L S PPDS EQQ  ITAIQN+DGYNWGYNPVLWGVPKGSYASN NGPCR IEFRKMV
Sbjct: 398  IEKLNSFPPDSEEQQALITAIQNEDGYNWGYNPVLWGVPKGSYASNANGPCRIIEFRKMV 457

Query: 361  QAFNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPGYYLRRNTNGDIEHSTCVNNTASE 540
            QA NRIGLRVV DVVYNH+  SG FDD SVLDKIVPGYYLRRN +G IE+STCVNNTASE
Sbjct: 458  QALNRIGLRVVSDVVYNHINASGHFDDFSVLDKIVPGYYLRRNVDGGIENSTCVNNTASE 517

Query: 541  HFMVERLIVDDLLCWAVDYKVDGFRFDLMGHIMKHTMVKAKSMLQGLSKDKSGVDGASIY 720
            HFMVERLI+DDL CWAV YK+DGFRFDLMGHIMK TMVKAKS+L  LSKDK+GVDG SIY
Sbjct: 518  HFMVERLILDDLKCWAVHYKIDGFRFDLMGHIMKRTMVKAKSLLNSLSKDKNGVDGPSIY 577

Query: 721  IYGEGWDFGEVAKNGRGTNASQFNLSNTGIGSFNDRIRDALLGGSPFGHPLQQGFVTGLA 900
            IYGEGWDFGEVA +GRG NASQFNL  +GIGSFNDRIRDALLGGSPFGHPL QGFVTGL 
Sbjct: 578  IYGEGWDFGEVANSGRGINASQFNLFGSGIGSFNDRIRDALLGGSPFGHPLHQGFVTGLY 637

Query: 901  LQANGYDHGSKSDEERTLAVLKDHIQVGMAANLKDYVLINCDGQEVKGSEVSTYDGVPVG 1080
            L+ NG+D G K++ ER LAV KDHIQVGMAANLKD+VL NCDG+EVKGSEV  +D  PVG
Sbjct: 638  LEPNGHDLGDKANVERMLAVSKDHIQVGMAANLKDFVLTNCDGEEVKGSEVLLHDRKPVG 697

Query: 1081 YSSRPTETVNYVSAHDNETLFDIVSLKTPMEISVDERCRINHLATSIIALSQGIPFFHSG 1260
            Y+S P ETVNYVSAHDNETLFDI+SLKTP +ISV+ERCR+NHLATS+IALSQGIPFFHSG
Sbjct: 698  YASSPVETVNYVSAHDNETLFDIISLKTPKDISVEERCRMNHLATSVIALSQGIPFFHSG 757

Query: 1261 DEMLRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKEKNEQNWPLIKPRLADPSFKP 1440
            DEMLRSKS+DRDSYNSGDWFNRLDFSY+SNNWGVGLPPKEKNE NWPLIKPRLADPS+KP
Sbjct: 758  DEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPLIKPRLADPSYKP 817

Query: 1441 QNCHILAAVENFLNLLQIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGVIVMGIEDGHN 1620
            Q  HILAAVENF NL+QIRYSSPLFRLRTAN+IQERVRFHNTGPSW+PG+IVM IEDGH 
Sbjct: 818  QKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQERVRFHNTGPSWIPGLIVMSIEDGHQ 877

Query: 1621 GVPGLSQLDTTYSYIIVIVNVCPTEVSFASPALRAKTLELHPVQMMSTDNIVKKSTYDAS 1800
            GVPGLSQLD  YSYI+VI+N  PT+VSFA+PALRA++L+LHP+QM STD++VK STYDAS
Sbjct: 878  GVPGLSQLDPIYSYIVVIINPGPTDVSFANPALRARSLQLHPIQMNSTDDVVKNSTYDAS 937

Query: 1801 SGYFKLPPRTTAIFVEPR 1854
            SG F +P RTT++FVEPR
Sbjct: 938  SGCFNVPARTTSVFVEPR 955