BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Acanthopanax21_contig00011967
         (1003 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_017217239.1| PREDICTED: E3 ubiquitin-protein ligase Os04g...   361   e-121
ref|XP_002277896.1| PREDICTED: E3 ubiquitin-protein ligase Os04g...   344   e-114
gb|POF24841.1| e3 ubiquitin-protein ligase [Quercus suber]            332   e-110
ref|XP_023923073.1| E3 ubiquitin-protein ligase Os04g0590900-lik...   332   e-109
ref|XP_017972774.1| PREDICTED: E3 ubiquitin-protein ligase Os04g...   331   e-109
gb|EOY23974.1| RING/U-box superfamily protein, putative [Theobro...   330   e-108
ref|XP_021281807.1| E3 ubiquitin-protein ligase Os04g0590900-lik...   326   e-107
ref|XP_019056071.1| PREDICTED: E3 ubiquitin-protein ligase Os04g...   321   e-105
ref|XP_011018550.1| PREDICTED: RING-H2 finger protein ATL52-like...   320   e-104
gb|PNT54428.1| hypothetical protein POPTR_001G141200v3 [Populus ...   320   e-104
ref|XP_006368390.1| hypothetical protein POPTR_0001s02360g [Popu...   320   e-104
ref|XP_018811969.1| PREDICTED: E3 ubiquitin-protein ligase Os04g...   318   e-104
emb|CAN78205.1| hypothetical protein VITISV_014334 [Vitis vinifera]   341   e-103
ref|XP_011028171.1| PREDICTED: E3 ubiquitin-protein ligase Os04g...   306   5e-99
gb|PNT44581.1| hypothetical protein POPTR_003G093100v3 [Populus ...   303   8e-98
ref|XP_017234730.1| PREDICTED: E3 ubiquitin-protein ligase Os04g...   300   1e-97
ref|XP_017234728.1| PREDICTED: E3 ubiquitin-protein ligase Os04g...   300   2e-97
ref|XP_022159853.1| RING-H2 finger protein ATL52-like isoform X1...   303   8e-97
ref|XP_022159854.1| E3 ubiquitin-protein ligase Os04g0590900-lik...   300   1e-96
dbj|GAV82564.1| zf-RING_2 domain-containing protein [Cephalotus ...   297   8e-96

>ref|XP_017217239.1| PREDICTED: E3 ubiquitin-protein ligase Os04g0590900-like [Daucus
           carota subsp. sativus]
 gb|KZN11637.1| hypothetical protein DCAR_004293 [Daucus carota subsp. sativus]
          Length = 356

 Score =  361 bits (926), Expect = e-121
 Identities = 174/265 (65%), Positives = 204/265 (76%), Gaps = 5/265 (1%)
 Frame = +2

Query: 26  RENHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGTDCSVCLSE 205
           RE HD  VELEEN DPSNHEPWH +N+GL+E LIKSITVCKY+K DGLIEGTDCSVCLSE
Sbjct: 89  REMHDSTVELEENHDPSNHEPWHSSNDGLEEVLIKSITVCKYRKGDGLIEGTDCSVCLSE 148

Query: 206 FQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPPPVTETPMSN 385
           F+E+ENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANI H +   +Q P PV+E PM +
Sbjct: 149 FEENENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIVHVSTFTVQPPLPVSEIPMEH 208

Query: 386 EIFSHGHGESTDVDTDVGEEDIMQGDIVPKTPTRVLSDLGNPEQRDTIIEISEVEYQQIR 565
           EI    H ES  V+ D+GEE++ QGD +PKTPTR   +LGN   RD II+I E EYQQ+R
Sbjct: 209 EIHFPDHRESDIVEDDIGEEELTQGDEIPKTPTRADRNLGNSANRDFIIDIREGEYQQVR 268

Query: 566 RSASMSHLCQARVSIADVMCINQEEEDCGMLQGDVGSSK-----MSKCSHRNRVLHCVTS 730
           RS SM++LCQ RVSIADV+C++ +EE    +  D GSSK     +SK SH+NR LHC T 
Sbjct: 269 RSVSMNNLCQTRVSIADVICLDHDEEANEFMYEDAGSSKKPVAELSKSSHKNRGLHCATD 328

Query: 731 PVGMKRSFSSGRFLFPRRGRGQNVV 805
           P  +KRSFSSGRF FP+  R Q +V
Sbjct: 329 PAAIKRSFSSGRFFFPKCSREQFIV 353


>ref|XP_002277896.1| PREDICTED: E3 ubiquitin-protein ligase Os04g0590900 [Vitis
           vinifera]
          Length = 375

 Score =  344 bits (882), Expect = e-114
 Identities = 171/278 (61%), Positives = 208/278 (74%), Gaps = 16/278 (5%)
 Frame = +2

Query: 23  RRENHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGTDCSVCLS 202
           RREN D N ELE+N +P+ HEPWHVA  GLDEALIKSIT+CKYKK DGL+EGTDCSVCLS
Sbjct: 88  RRENQDINEELEDNHNPALHEPWHVATTGLDEALIKSITLCKYKKGDGLVEGTDCSVCLS 147

Query: 203 EFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPPPVTETPMS 382
           EF+E+E+LRLLPKCSHAFHV+CIDTWL+SHSNCPLCRANI      P QLPPPV E+P++
Sbjct: 148 EFEEEESLRLLPKCSHAFHVQCIDTWLKSHSNCPLCRANIVPTTVSPTQLPPPVMESPLN 207

Query: 383 NEIFSHGHGE------STDVDTDVGEEDIMQGDIVPKTPTRVLSDLGNPEQRDTIIEISE 544
           NE       E        D++    EE+IM+ +++PKTP+RV SDLGN E+RDTIIEI +
Sbjct: 208 NEPSQQSQLEHENVAVGEDLERVAEEEEIMRNEVIPKTPSRVFSDLGNSEERDTIIEIRD 267

Query: 545 VEYQQIRRSASMSHLCQARVSIADVMCINQEEE----DCG-MLQGDVGSSK-----MSKC 694
              QQIRRS S+ + CQ R+S+AD++ +N++E+    DC     G VGSSK     +SK 
Sbjct: 268 EGEQQIRRSISLDYSCQTRLSVADILLMNEDEDPQMGDCDCQFPGHVGSSKVAVGEISKY 327

Query: 695 SHRNRVLHCVTSPVGMKRSFSSGRFLFPRRGRGQNVVI 808
           SHRNRVLHCV SPV MKRSFSSGR LF R  RG++ +I
Sbjct: 328 SHRNRVLHCVMSPVSMKRSFSSGRLLFTRHARGRDGII 365


>gb|POF24841.1| e3 ubiquitin-protein ligase [Quercus suber]
          Length = 330

 Score =  332 bits (852), Expect = e-110
 Identities = 169/285 (59%), Positives = 201/285 (70%), Gaps = 17/285 (5%)
 Frame = +2

Query: 5   NTDSARRRENHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGTD 184
           N DSAR RE+H+ N ELEENQ+PS HEPWH+   GLDEALIKSI V KYKK +GL+EGTD
Sbjct: 49  NPDSARSREDHESNEELEENQNPSLHEPWHIVTTGLDEALIKSIAVYKYKKGEGLVEGTD 108

Query: 185 CSVCLSEFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPPPV 364
           CSVCLSEF+EDE++RLLPKCSHAFH+ CIDTWL+SH+NCPLCRANI   NA P+ LP PV
Sbjct: 109 CSVCLSEFEEDESIRLLPKCSHAFHLPCIDTWLKSHANCPLCRANIVSFNATPIPLPAPV 168

Query: 365 TETPMSNEIFSHGHGE------STDVDTDVGEEDIMQGDIVPKTPTRVLSDLGNPEQRDT 526
           TE P +NE       E      + D+   V EE+ ++GDIVPK+P R  SDLGN E+RDT
Sbjct: 169 TELPSTNETLPISQPENENITLAQDIAGGVTEEESVRGDIVPKSPFRAFSDLGNSEERDT 228

Query: 527 IIEISEVEYQQIRRSASMSHLCQARVSIADVMCINQEE----EDCG-------MLQGDVG 673
           IIEI +  Y  IRRS SM   CQ+RVS+AD++C+NQ E    E+C         L G+VG
Sbjct: 229 IIEIRDGGYHHIRRSFSMDLSCQSRVSVADILCMNQGEDVQVEECSAEASSSKQLAGEVG 288

Query: 674 SSKMSKCSHRNRVLHCVTSPVGMKRSFSSGRFLFPRRGRGQNVVI 808
                K SHRNRVLH V SP+ MKR FSSGRF   R GR  + +I
Sbjct: 289 -----KSSHRNRVLHFVMSPIAMKRQFSSGRFFLTRHGRRPHAII 328


>ref|XP_023923073.1| E3 ubiquitin-protein ligase Os04g0590900-like [Quercus suber]
 ref|XP_023923078.1| E3 ubiquitin-protein ligase Os04g0590900-like [Quercus suber]
          Length = 363

 Score =  332 bits (852), Expect = e-109
 Identities = 169/285 (59%), Positives = 201/285 (70%), Gaps = 17/285 (5%)
 Frame = +2

Query: 5   NTDSARRRENHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGTD 184
           N DSAR RE+H+ N ELEENQ+PS HEPWH+   GLDEALIKSI V KYKK +GL+EGTD
Sbjct: 82  NPDSARSREDHESNEELEENQNPSLHEPWHIVTTGLDEALIKSIAVYKYKKGEGLVEGTD 141

Query: 185 CSVCLSEFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPPPV 364
           CSVCLSEF+EDE++RLLPKCSHAFH+ CIDTWL+SH+NCPLCRANI   NA P+ LP PV
Sbjct: 142 CSVCLSEFEEDESIRLLPKCSHAFHLPCIDTWLKSHANCPLCRANIVSFNATPIPLPAPV 201

Query: 365 TETPMSNEIFSHGHGE------STDVDTDVGEEDIMQGDIVPKTPTRVLSDLGNPEQRDT 526
           TE P +NE       E      + D+   V EE+ ++GDIVPK+P R  SDLGN E+RDT
Sbjct: 202 TELPSTNETLPISQPENENITLAQDIAGGVTEEESVRGDIVPKSPFRAFSDLGNSEERDT 261

Query: 527 IIEISEVEYQQIRRSASMSHLCQARVSIADVMCINQEE----EDCG-------MLQGDVG 673
           IIEI +  Y  IRRS SM   CQ+RVS+AD++C+NQ E    E+C         L G+VG
Sbjct: 262 IIEIRDGGYHHIRRSFSMDLSCQSRVSVADILCMNQGEDVQVEECSAEASSSKQLAGEVG 321

Query: 674 SSKMSKCSHRNRVLHCVTSPVGMKRSFSSGRFLFPRRGRGQNVVI 808
                K SHRNRVLH V SP+ MKR FSSGRF   R GR  + +I
Sbjct: 322 -----KSSHRNRVLHFVMSPIAMKRQFSSGRFFLTRHGRRPHAII 361


>ref|XP_017972774.1| PREDICTED: E3 ubiquitin-protein ligase Os04g0590900 [Theobroma
           cacao]
          Length = 370

 Score =  331 bits (848), Expect = e-109
 Identities = 173/286 (60%), Positives = 200/286 (69%), Gaps = 18/286 (6%)
 Frame = +2

Query: 5   NTDSARRRENHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGTD 184
           N D +RRRENHDP   LE+N  PS HEPW  +  GLDEALIKSITVCKYKK DGLIEGTD
Sbjct: 83  NVDPSRRRENHDPGEVLEDNHSPSVHEPWQTSTTGLDEALIKSITVCKYKKGDGLIEGTD 142

Query: 185 CSVCLSEFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPPPV 364
           CSVCLSEFQEDE+LRLLPKCSHAFHV CIDTWLRSHSNCPLCRANI   +A PL LPP V
Sbjct: 143 CSVCLSEFQEDESLRLLPKCSHAFHVHCIDTWLRSHSNCPLCRANIIFISASPLPLPPAV 202

Query: 365 TETPMSNEIFSHGH---GESTDVDTDVG-----EEDIMQGDI--VPKTPTRVLSDLGNPE 514
           TE+P  NE          E      D G     E D  Q D+  +PKTP+R  SDLGN +
Sbjct: 203 TESPPGNESLQDRQPVTNEYAAAAQDSGRRSMQEVDRFQSDLSRLPKTPSRAFSDLGNAQ 262

Query: 515 QRDTIIEISEVEYQQIRRSASMSHLCQARVSIADVMCINQEE----EDCGMLQGDVGSSK 682
            RD++IEI E  YQQ+RRS SM H CQ+++S+AD++ +NQEE    E+C    G  GSSK
Sbjct: 263 GRDSVIEIREESYQQMRRSVSMDHPCQSQLSVADILHMNQEEDSRVEECHFSSGIAGSSK 322

Query: 683 M----SKCSHRNRVLHCVTSPVGMKRSFSSGRFLFPRRGRGQNVVI 808
                SK S++ RVLHCV +PV MKRSFSSGRF   R+GR ++  I
Sbjct: 323 QSAEGSKLSNKMRVLHCVMNPVAMKRSFSSGRFFLTRQGRVRDPAI 368


>gb|EOY23974.1| RING/U-box superfamily protein, putative [Theobroma cacao]
          Length = 370

 Score =  330 bits (845), Expect = e-108
 Identities = 172/286 (60%), Positives = 200/286 (69%), Gaps = 18/286 (6%)
 Frame = +2

Query: 5   NTDSARRRENHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGTD 184
           N D +RRRENHDP   LE+N  PS HEPW  +  GLDEALIKSITVCKYKK DGLIEGTD
Sbjct: 83  NVDPSRRRENHDPGEVLEDNHSPSVHEPWQTSTTGLDEALIKSITVCKYKKGDGLIEGTD 142

Query: 185 CSVCLSEFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPPPV 364
           CSVCLSEFQEDE+LRLLPKCSHAFHV CIDTWLRSHSNCPLCRANI   +A PL LPP +
Sbjct: 143 CSVCLSEFQEDESLRLLPKCSHAFHVHCIDTWLRSHSNCPLCRANIIFISASPLPLPPAL 202

Query: 365 TETPMSNEIFSHGH---GESTDVDTDVG-----EEDIMQGDI--VPKTPTRVLSDLGNPE 514
           TE+P  NE          E      D G     E D  Q D+  +PKTP+R  SDLGN +
Sbjct: 203 TESPPGNESLQDRQPVTNEYAAAAQDSGRRSMQEVDRFQSDLSRLPKTPSRAFSDLGNAQ 262

Query: 515 QRDTIIEISEVEYQQIRRSASMSHLCQARVSIADVMCINQEE----EDCGMLQGDVGSSK 682
            RD++IEI E  YQQ+RRS SM H CQ+++S+AD++ +NQEE    E+C    G  GSSK
Sbjct: 263 GRDSVIEIREESYQQMRRSVSMDHPCQSQLSVADILHMNQEEDSRVEECHFSSGIAGSSK 322

Query: 683 M----SKCSHRNRVLHCVTSPVGMKRSFSSGRFLFPRRGRGQNVVI 808
                SK S++ RVLHCV +PV MKRSFSSGRF   R+GR ++  I
Sbjct: 323 QSAEGSKLSNKMRVLHCVMNPVAMKRSFSSGRFFLTRQGRVRDPAI 368


>ref|XP_021281807.1| E3 ubiquitin-protein ligase Os04g0590900-like [Herrania umbratica]
          Length = 370

 Score =  326 bits (836), Expect = e-107
 Identities = 172/286 (60%), Positives = 198/286 (69%), Gaps = 18/286 (6%)
 Frame = +2

Query: 5   NTDSARRRENHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGTD 184
           N DS+RRRENHDP   LE+N  PS HEPW  +  GLDEALIKSITVCKYKK DGLIEGTD
Sbjct: 83  NVDSSRRRENHDPGEVLEDNHSPSVHEPWQTSTTGLDEALIKSITVCKYKKGDGLIEGTD 142

Query: 185 CSVCLSEFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPPPV 364
           CSVCLSEFQEDE+LRLLPKCSHAFHV CIDTWLRSHSNCPLCRANI   +A  L LPP V
Sbjct: 143 CSVCLSEFQEDESLRLLPKCSHAFHVHCIDTWLRSHSNCPLCRANIIFISASLLPLPPAV 202

Query: 365 TETPMSNEIFSHGH---GESTDVDTDVG-----EEDIMQGDI--VPKTPTRVLSDLGNPE 514
           TE+P  NE          E      D G     E D  Q D+  +PKTP+R  SDL N +
Sbjct: 203 TESPPGNESLQDRQPVTNEYAAAAQDSGRGSMQEVDRFQSDLSRLPKTPSRAFSDLSNAQ 262

Query: 515 QRDTIIEISEVEYQQIRRSASMSHLCQARVSIADVMCINQEE----EDCGMLQGDVGSSK 682
            RD++IEI E  YQQ+RRS SM H CQ++ S+AD++ +NQEE    E+C    G  GSSK
Sbjct: 263 GRDSVIEIREESYQQMRRSVSMDHPCQSQTSVADILHMNQEEDSRDEECHFSSGIAGSSK 322

Query: 683 M----SKCSHRNRVLHCVTSPVGMKRSFSSGRFLFPRRGRGQNVVI 808
                SK S++ RVLHCV +PV MKRSFSSGRF   R+GR ++  I
Sbjct: 323 QSAEGSKLSNKMRVLHCVMNPVAMKRSFSSGRFFLTRQGRVRDPAI 368


>ref|XP_019056071.1| PREDICTED: E3 ubiquitin-protein ligase Os04g0590900 [Nelumbo
           nucifera]
          Length = 369

 Score =  321 bits (822), Expect = e-105
 Identities = 167/285 (58%), Positives = 200/285 (70%), Gaps = 17/285 (5%)
 Frame = +2

Query: 5   NTDSARRREN-HDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGT 181
           N DS RRR N HDPN E ++ ++  NHE W V+  GLDEALIKSITVCKYK+ DGLI+G 
Sbjct: 84  NLDSLRRRGNDHDPNEEFDDTRN-LNHEQWQVSTGGLDEALIKSITVCKYKRGDGLIDGA 142

Query: 182 DCSVCLSEFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPPP 361
           DCSVCLSEFQEDE+LRLLPKCSHAFHV CIDTWL+SHSNCPLCRANI   N LPLQ  PP
Sbjct: 143 DCSVCLSEFQEDESLRLLPKCSHAFHVTCIDTWLKSHSNCPLCRANIVSPNPLPLQSLPP 202

Query: 362 VTETPMSNEIFSHGHGEST--------DVDTDVGEEDIMQGDIVPKTPTRVLSDLGNPEQ 517
           V ET   N        E+T        D + D GEE    G   PKTP R +SDLG+ +Q
Sbjct: 203 VPETRPENVSSIERRQENTTVTVVAVEDTEWDSGEETTQDGQAAPKTPLRAMSDLGDAQQ 262

Query: 518 RDTIIEISEVEYQQIRRSASMSHLCQARVSIADVMCINQEE---EDCGMLQGDVGSSKM- 685
           RDTIIEI +  +Q+IRRS SM   CQ R+SIAD++C++Q+E   E+   L  +VGSSK  
Sbjct: 263 RDTIIEIRDEGFQKIRRSVSMDSSCQGRLSIADMLCMSQDEDSQEEDNQLPAEVGSSKQS 322

Query: 686 ----SKCSHRNRVLHCVTSPVGMKRSFSSGRFLFPRRGRGQNVVI 808
               SK + +NRVL C+TSP+ MKRSFSSGRF+  R GRG++ +I
Sbjct: 323 WGEHSKSNSKNRVLLCITSPIAMKRSFSSGRFMLTRHGRGKDSII 367


>ref|XP_011018550.1| PREDICTED: RING-H2 finger protein ATL52-like [Populus euphratica]
 ref|XP_011018551.1| PREDICTED: RING-H2 finger protein ATL52-like [Populus euphratica]
          Length = 368

 Score =  320 bits (820), Expect = e-104
 Identities = 171/282 (60%), Positives = 197/282 (69%), Gaps = 19/282 (6%)
 Frame = +2

Query: 2   RNTDSARRR-ENHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEG 178
           RN D ARRR ENH  N E E + +PS HEPWH A  GLDEALI+SITVCKYKK DGLI+G
Sbjct: 82  RNNDPARRRDENHGQNEEFEGDHNPSLHEPWHAATTGLDEALIRSITVCKYKKGDGLIDG 141

Query: 179 TDCSVCLSEFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPP 358
           TDCSVCLSEFQEDE++RLLPKCSHAFHV CIDTWLRSHSNCPLCRANI   +A P QLPP
Sbjct: 142 TDCSVCLSEFQEDESIRLLPKCSHAFHVPCIDTWLRSHSNCPLCRANIVFFSASPSQLPP 201

Query: 359 PVTETPMSNEIFSHGHGESTDVDTDVGEEDIMQGDIV-------PKTPTRVLSDLGNPEQ 517
           PVTETP  NE + +  G   +V    G E + + + V       PKTP RV SDLGN E+
Sbjct: 202 PVTETPQGNESWQNSQGSDDNVAATQGTERVARDEQVMQNPAGDPKTPLRVFSDLGNLEE 261

Query: 518 RDTIIEISEVE-YQQIRRSASMSHLCQARVSIADVMCINQE----EEDCGMLQGDVGSSK 682
           RDTIIE+ +++ YQ+IRRS SM H CQ   S+AD++ +NQ+     ED     GDVGSSK
Sbjct: 262 RDTIIEMRDIDGYQRIRRSVSMDHPCQCDASVADILRMNQDGVVRVED---RSGDVGSSK 318

Query: 683 MS------KCSHRNRVLHCVTSPVGMKRSFSSGRFLFPRRGR 790
            S        S+R RVL CV +PV MKRSFSSGRF     GR
Sbjct: 319 HSAEDSRFTSSNRRRVLQCVLNPVTMKRSFSSGRFFPTGHGR 360


>gb|PNT54428.1| hypothetical protein POPTR_001G141200v3 [Populus trichocarpa]
          Length = 368

 Score =  320 bits (819), Expect = e-104
 Identities = 169/281 (60%), Positives = 198/281 (70%), Gaps = 19/281 (6%)
 Frame = +2

Query: 5   NTDSARRRE-NHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGT 181
           N D ARRR+ NH  N E E + +PS HEPWH A  GLDEALI+SITVCKYKK DGLI+GT
Sbjct: 83  NNDPARRRDQNHGQNEEFEGDHNPSLHEPWHAATTGLDEALIRSITVCKYKKGDGLIDGT 142

Query: 182 DCSVCLSEFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPPP 361
           DCSVCLSEFQEDE++RLLPKCSHAFHV CIDTWLRSHSNCPLCRANI   +A P QLPPP
Sbjct: 143 DCSVCLSEFQEDESIRLLPKCSHAFHVPCIDTWLRSHSNCPLCRANIVFFSASPPQLPPP 202

Query: 362 VTETPMSNEIFSHGHGESTDVDTDVGEEDIMQGDIV-------PKTPTRVLSDLGNPEQR 520
           V ETP  NE + +    + +V    G E + + + V       PKTP RV SDLGN E+R
Sbjct: 203 VAETPRGNESWQNSQRSNDNVAATQGTERVARDEQVMQNPAGDPKTPWRVFSDLGNLEER 262

Query: 521 DTIIEISEVE-YQQIRRSASMSHLCQARVSIADVMCINQE----EEDCGMLQGDVGSSKM 685
           DTIIE+ +++ YQ+IRRS SM H CQ+  S+AD++ +NQ+     EDC    GDVGSSK 
Sbjct: 263 DTIIEMRDIDGYQRIRRSVSMDHPCQSHASVADILRMNQDGVVRVEDC---SGDVGSSKH 319

Query: 686 S------KCSHRNRVLHCVTSPVGMKRSFSSGRFLFPRRGR 790
           S        S+R RVLHCV +PV MKRSFSSGRF     GR
Sbjct: 320 SAEDSKFTSSNRRRVLHCVLNPVTMKRSFSSGRFFPTGHGR 360


>ref|XP_006368390.1| hypothetical protein POPTR_0001s02360g [Populus trichocarpa]
          Length = 368

 Score =  320 bits (819), Expect = e-104
 Identities = 169/281 (60%), Positives = 198/281 (70%), Gaps = 19/281 (6%)
 Frame = +2

Query: 5   NTDSARRRE-NHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGT 181
           N D ARRR+ NH  N E E + +PS HEPWH A  GLDEALI+SITVCKYKK DGLI+GT
Sbjct: 83  NNDPARRRDQNHGQNEEFEGDHNPSLHEPWHAATTGLDEALIRSITVCKYKKGDGLIDGT 142

Query: 182 DCSVCLSEFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPPP 361
           DCSVCLSEFQEDE++RLLPKCSHAFHV CIDTWLRSHSNCPLCRANI   +A P QLPPP
Sbjct: 143 DCSVCLSEFQEDESIRLLPKCSHAFHVPCIDTWLRSHSNCPLCRANIVFFSASPPQLPPP 202

Query: 362 VTETPMSNEIFSHGHGESTDVDTDVGEEDIMQGDIV-------PKTPTRVLSDLGNPEQR 520
           V ETP  NE + +    + +V    G E + + + V       PKTP RV SDLGN E+R
Sbjct: 203 VAETPRGNESWQNSQRSNDNVAATQGTERVARDEQVMQNPAGDPKTPWRVFSDLGNLEER 262

Query: 521 DTIIEISEVE-YQQIRRSASMSHLCQARVSIADVMCINQE----EEDCGMLQGDVGSSKM 685
           DTIIE+ +++ YQ+IRRS SM H CQ+  S+AD++ +NQ+     EDC    GDVGSSK 
Sbjct: 263 DTIIEMRDIDGYQRIRRSVSMDHPCQSHASVADILRMNQDGVVRVEDC---SGDVGSSKH 319

Query: 686 S------KCSHRNRVLHCVTSPVGMKRSFSSGRFLFPRRGR 790
           S        S+R RVLHCV +PV MKRSFSSGRF     GR
Sbjct: 320 SAEDSKFTSSNRRRVLHCVLNPVTMKRSFSSGRFFPTGHGR 360


>ref|XP_018811969.1| PREDICTED: E3 ubiquitin-protein ligase Os04g0590900-like [Juglans
           regia]
 ref|XP_018811970.1| PREDICTED: E3 ubiquitin-protein ligase Os04g0590900-like [Juglans
           regia]
          Length = 367

 Score =  318 bits (815), Expect = e-104
 Identities = 163/284 (57%), Positives = 200/284 (70%), Gaps = 16/284 (5%)
 Frame = +2

Query: 5   NTDSARRRENHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGTD 184
           N DSARR ++HD + E E+NQ  S HEPWHV   GLDEALIKSITVCKYKK +GL+EGTD
Sbjct: 83  NGDSARR-DDHDLSEESEDNQSHSLHEPWHVVTAGLDEALIKSITVCKYKKGEGLVEGTD 141

Query: 185 CSVCLSEFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPPPV 364
           CSVCLSEF+EDE++RLLPKCSHAFH+ CIDTWL+SHSNCPLCRANI      PL LP PV
Sbjct: 142 CSVCLSEFEEDESIRLLPKCSHAFHLPCIDTWLKSHSNCPLCRANIVSFGGTPLPLPAPV 201

Query: 365 TETPMSNEIF------SHGHGESTDVDTDVGEEDIMQGDIVPKTPTRVLSDLGNPEQRDT 526
           T+ P SNE        +     + D+++ V EE+++ GD  PKT  R  SDLGN E+RDT
Sbjct: 202 TDIPSSNETLPGTQQANENVTLAQDLESYVREEEMVHGDTAPKTSVRAFSDLGNSEERDT 261

Query: 527 IIEISEVEYQQIRRSASMSHLCQARVSIADVMCINQEEEDCGML----QGDVGSSKM--- 685
           IIEI +  YQ +RRS SM  LCQ+R S+AD++C++++ ED  ++     GD  SSK    
Sbjct: 262 IIEIRDGGYQHVRRSFSMDLLCQSRPSVADILCMDEDHEDVQVVVEDCVGDSSSSKQLAA 321

Query: 686 ---SKCSHRNRVLHCVTSPVGMKRSFSSGRFLFPRRGRGQNVVI 808
               K S ++RVLH VTSP+ MKRSFSSGRF   R GR +  VI
Sbjct: 322 GVEEKSSSKSRVLHMVTSPIAMKRSFSSGRFFLTRHGRRRQTVI 365


>emb|CAN78205.1| hypothetical protein VITISV_014334 [Vitis vinifera]
          Length = 1377

 Score =  341 bits (874), Expect = e-103
 Identities = 171/278 (61%), Positives = 206/278 (74%), Gaps = 16/278 (5%)
 Frame = +2

Query: 23   RRENHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGTDCSVCLS 202
            RREN D N ELE+N +P+ HEPWHVA  GLDEALIKSIT+CKYKK DGL+EGTDCSVCLS
Sbjct: 1090 RRENQDINEELEDNHNPALHEPWHVATTGLDEALIKSITLCKYKKGDGLVEGTDCSVCLS 1149

Query: 203  EFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPPPVTETPMS 382
            EF+EDE+LRLLPKCSHAFHV+CIDTWL+SHSNCPLCRANI      P QLPPPV E P++
Sbjct: 1150 EFEEDESLRLLPKCSHAFHVQCIDTWLKSHSNCPLCRANIVPTTVSPTQLPPPVMEXPLN 1209

Query: 383  NEIFSHGHGE------STDVDTDVGEEDIMQGDIVPKTPTRVLSDLGNPEQRDTIIEISE 544
            NE       E        D++    EE+IM+ +++PKTP+RV SDLGN E+RDTIIEI +
Sbjct: 1210 NEPSQQSQLEHENVAVGEDLERVAEEEEIMRNEVIPKTPSRVFSDLGNSEERDTIIEIRD 1269

Query: 545  VEYQQIRRSASMSHLCQARVSIADVMCINQEEE----DCG-MLQGDVGSSK-----MSKC 694
               QQIRRS S+ +  Q R+S+AD++ +N++E+    DC     G VGSSK     +SK 
Sbjct: 1270 EGEQQIRRSISLDYSXQTRLSVADILLMNEDEDPQMGDCDCQFPGHVGSSKVAVGEISKY 1329

Query: 695  SHRNRVLHCVTSPVGMKRSFSSGRFLFPRRGRGQNVVI 808
            SHRNRVLHCV SPV MKRSFSSGR LF R  RG++ +I
Sbjct: 1330 SHRNRVLHCVMSPVSMKRSFSSGRLLFTRHARGRDGII 1367


>ref|XP_011028171.1| PREDICTED: E3 ubiquitin-protein ligase Os04g0590900-like [Populus
           euphratica]
          Length = 368

 Score =  306 bits (783), Expect = 5e-99
 Identities = 164/281 (58%), Positives = 195/281 (69%), Gaps = 19/281 (6%)
 Frame = +2

Query: 5   NTDSARRRE-NHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGT 181
           N DSARRR+ NH  N E E++ + S HEPWH A  G+DEALI+SITVCKY+K DGLIEGT
Sbjct: 83  NDDSARRRDQNHGQNEEFEDDHNSSLHEPWHAATTGVDEALIRSITVCKYRKGDGLIEGT 142

Query: 182 DCSVCLSEFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPPP 361
           DCSVCLSEFQEDE++RLLPKCSHAFHV CIDTWLRSHSNCPLCRANI   +A   QLPPP
Sbjct: 143 DCSVCLSEFQEDESVRLLPKCSHAFHVPCIDTWLRSHSNCPLCRANIVFFSASLPQLPPP 202

Query: 362 VTETPMSNEIFSHGHGESTDVDTDVGEEDIMQGDI-------VPKTPTRVLSDLGNPEQR 520
           VT+TP  NE        + +V      E + + +         PKTP+RV SD GN E+R
Sbjct: 203 VTDTPQGNESLQESQRANDNVSVTQDTERVARDEQATQNLAGAPKTPSRVFSDSGNLEER 262

Query: 521 DTIIEISEVE-YQQIRRSASMSHLCQARVSIADVMCINQEE----EDCGMLQGDVGSSK- 682
           D IIE+ +++  Q+IRRS SM H CQ+  SIA+++ +NQ+E    EDC     DVGSSK 
Sbjct: 263 DIIIEMRDIDRNQRIRRSFSMDHPCQSHTSIAEIIRMNQDEVVPVEDC---TADVGSSKH 319

Query: 683 ---MSKC--SHRNRVLHCVTSPVGMKRSFSSGRFLFPRRGR 790
              +SKC  S   RVLHCV +PV MKRSFSSGRF   R GR
Sbjct: 320 SAEISKCVSSSSRRVLHCVLNPVTMKRSFSSGRFFLTRHGR 360


>gb|PNT44581.1| hypothetical protein POPTR_003G093100v3 [Populus trichocarpa]
          Length = 368

 Score =  303 bits (775), Expect = 8e-98
 Identities = 163/286 (56%), Positives = 197/286 (68%), Gaps = 19/286 (6%)
 Frame = +2

Query: 5   NTDSARRRE-NHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGT 181
           N  SARRR+ NH  N E E++ + S HEPWH A  G+DEALI+SITVCKYKK DGLIEGT
Sbjct: 83  NDYSARRRDQNHGQNEEFEDDHNSSLHEPWHAATTGVDEALIRSITVCKYKKGDGLIEGT 142

Query: 182 DCSVCLSEFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPPP 361
           DCSVCLSEF+EDE++RLLPKCSHAFHV CIDTWLRSHSNCPLCRANI   +A   QLPPP
Sbjct: 143 DCSVCLSEFEEDESIRLLPKCSHAFHVPCIDTWLRSHSNCPLCRANIVFFSASLPQLPPP 202

Query: 362 VTETPMSNEIFSHGHGESTDVDTDVGEEDIMQGDI-------VPKTPTRVLSDLGNPEQR 520
           VTETP  NE        + +V      E + + +         PKTP+RV  D GN E+R
Sbjct: 203 VTETPQGNESLQESQRANENVSVTQDTERVARDEQATQNLAGAPKTPSRVFRDSGNLEER 262

Query: 521 DTIIEISEVE-YQQIRRSASMSHLCQARVSIADVMCINQEE----EDCGMLQGDVGSSK- 682
           D IIE+ +++ YQ+IRRS SM H CQ+  SIA+++ +NQ+E    EDC    GDVGSSK 
Sbjct: 263 DIIIEMRDIDRYQRIRRSFSMDHPCQSHTSIAEIIRMNQDEVVPVEDC---SGDVGSSKH 319

Query: 683 ---MSKC--SHRNRVLHCVTSPVGMKRSFSSGRFLFPRRGRGQNVV 805
              +SKC  S   RVL+CV +PV MKRSFSSGRF     GR ++ +
Sbjct: 320 SAEISKCVSSSCRRVLNCVLNPVTMKRSFSSGRFFLTGHGRMRDTI 365


>ref|XP_017234730.1| PREDICTED: E3 ubiquitin-protein ligase Os04g0590900-like isoform X2
           [Daucus carota subsp. sativus]
 gb|KZN07418.1| hypothetical protein DCAR_008255 [Daucus carota subsp. sativus]
          Length = 309

 Score =  300 bits (768), Expect = 1e-97
 Identities = 143/223 (64%), Positives = 170/223 (76%)
 Frame = +2

Query: 11  DSARRRENHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGTDCS 190
           DS RR  ++ P+V+LE N  PSNHEPWHV+ +GLDE +IKSITV +YKK DGLIEGT+CS
Sbjct: 84  DSTRREIHNYPSVDLEGNYGPSNHEPWHVSGDGLDEVMIKSITVFEYKKGDGLIEGTECS 143

Query: 191 VCLSEFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPPPVTE 370
           VCLSEFQE ENLRLLPKCSHAFHVKCIDTWLR+HSNCPLCR ++ + + L LQ P PV E
Sbjct: 144 VCLSEFQESENLRLLPKCSHAFHVKCIDTWLRAHSNCPLCRGDVVNLSTLTLQPPLPVPE 203

Query: 371 TPMSNEIFSHGHGESTDVDTDVGEEDIMQGDIVPKTPTRVLSDLGNPEQRDTIIEISEVE 550
            PM+  I+  GH E+   + DV EE++ + D +PKTP R   DLGNP   D I E  E E
Sbjct: 204 APMNPGIYFQGHRETDIAEADVAEEELTEADEIPKTPARADRDLGNPADGDPIPETGEAE 263

Query: 551 YQQIRRSASMSHLCQARVSIADVMCINQEEEDCGMLQGDVGSS 679
           YQQIRRS SMS+LCQARVSIADVMC++Q+EED   +  D GSS
Sbjct: 264 YQQIRRSVSMSNLCQARVSIADVMCMDQDEEDSEFMSEDAGSS 306


>ref|XP_017234728.1| PREDICTED: E3 ubiquitin-protein ligase Os04g0590900-like isoform X1
           [Daucus carota subsp. sativus]
          Length = 327

 Score =  300 bits (768), Expect = 2e-97
 Identities = 143/223 (64%), Positives = 170/223 (76%)
 Frame = +2

Query: 11  DSARRRENHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGTDCS 190
           DS RR  ++ P+V+LE N  PSNHEPWHV+ +GLDE +IKSITV +YKK DGLIEGT+CS
Sbjct: 102 DSTRREIHNYPSVDLEGNYGPSNHEPWHVSGDGLDEVMIKSITVFEYKKGDGLIEGTECS 161

Query: 191 VCLSEFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANALPLQLPPPVTE 370
           VCLSEFQE ENLRLLPKCSHAFHVKCIDTWLR+HSNCPLCR ++ + + L LQ P PV E
Sbjct: 162 VCLSEFQESENLRLLPKCSHAFHVKCIDTWLRAHSNCPLCRGDVVNLSTLTLQPPLPVPE 221

Query: 371 TPMSNEIFSHGHGESTDVDTDVGEEDIMQGDIVPKTPTRVLSDLGNPEQRDTIIEISEVE 550
            PM+  I+  GH E+   + DV EE++ + D +PKTP R   DLGNP   D I E  E E
Sbjct: 222 APMNPGIYFQGHRETDIAEADVAEEELTEADEIPKTPARADRDLGNPADGDPIPETGEAE 281

Query: 551 YQQIRRSASMSHLCQARVSIADVMCINQEEEDCGMLQGDVGSS 679
           YQQIRRS SMS+LCQARVSIADVMC++Q+EED   +  D GSS
Sbjct: 282 YQQIRRSVSMSNLCQARVSIADVMCMDQDEEDSEFMSEDAGSS 324


>ref|XP_022159853.1| RING-H2 finger protein ATL52-like isoform X1 [Momordica charantia]
          Length = 457

 Score =  303 bits (776), Expect = 8e-97
 Identities = 158/276 (57%), Positives = 186/276 (67%), Gaps = 14/276 (5%)
 Frame = +2

Query: 5    NTDSARRRENHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGTD 184
            NT+   R  NHDPN E E+N +P+ HEPWH+A  GLDEALIKSITVCKYK+ DGL+EG+D
Sbjct: 174  NTNRLSRTGNHDPNEEFEDNHNPTLHEPWHIATTGLDEALIKSITVCKYKRGDGLVEGSD 233

Query: 185  CSVCLSEFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANA-LPLQLPP- 358
            C+VCLSEFQEDE+LRLLPKCSHAFH++CIDTW +SHSNCPLCRA I   +A  P QLPP 
Sbjct: 234  CAVCLSEFQEDESLRLLPKCSHAFHLQCIDTWFKSHSNCPLCRAYIVSFSASSPFQLPPS 293

Query: 359  ---PVTETPMSNEIFSHGH-GESTDVDTDVGEEDIMQGDIVPKTPTRVLSDLGNPEQRDT 526
               P+T   M     +  H   S D     G +D+MQ     K   R LSDLGN E+RDT
Sbjct: 294  TAVPITSEAMPETSHTTDHDAVSQDSGRSFGNDDLMQAHNCAKNSFRALSDLGNLEKRDT 353

Query: 527  IIEISEVEYQQIRRSASMSHLCQARVSIADVMCINQE---EEDCGMLQGDVGSSKM---- 685
            +IE+ +   QQIRRS SM H CQ    IAD++ +N++     D     GDVGSSK     
Sbjct: 354  VIELRDGRLQQIRRSISMDHCCQNHTIIADILQLNEDGFGAIDESGSSGDVGSSKHSVGE 413

Query: 686  -SKCSHRNRVLHCVTSPVGMKRSFSSGRFLFPRRGR 790
             SK SHR R+LHCV SPV MKRSFSSGRF   R GR
Sbjct: 414  DSKSSHRKRILHCVMSPVSMKRSFSSGRFSLTRHGR 449


>ref|XP_022159854.1| E3 ubiquitin-protein ligase Os04g0590900-like isoform X2 [Momordica
           charantia]
          Length = 366

 Score =  300 bits (767), Expect = 1e-96
 Identities = 156/270 (57%), Positives = 183/270 (67%), Gaps = 14/270 (5%)
 Frame = +2

Query: 23  RRENHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGTDCSVCLS 202
           R  NHDPN E E+N +P+ HEPWH+A  GLDEALIKSITVCKYK+ DGL+EG+DC+VCLS
Sbjct: 89  RTGNHDPNEEFEDNHNPTLHEPWHIATTGLDEALIKSITVCKYKRGDGLVEGSDCAVCLS 148

Query: 203 EFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAHANA-LPLQLPP----PVT 367
           EFQEDE+LRLLPKCSHAFH++CIDTW +SHSNCPLCRA I   +A  P QLPP    P+T
Sbjct: 149 EFQEDESLRLLPKCSHAFHLQCIDTWFKSHSNCPLCRAYIVSFSASSPFQLPPSTAVPIT 208

Query: 368 ETPMSNEIFSHGH-GESTDVDTDVGEEDIMQGDIVPKTPTRVLSDLGNPEQRDTIIEISE 544
              M     +  H   S D     G +D+MQ     K   R LSDLGN E+RDT+IE+ +
Sbjct: 209 SEAMPETSHTTDHDAVSQDSGRSFGNDDLMQAHNCAKNSFRALSDLGNLEKRDTVIELRD 268

Query: 545 VEYQQIRRSASMSHLCQARVSIADVMCINQE---EEDCGMLQGDVGSSKM-----SKCSH 700
              QQIRRS SM H CQ    IAD++ +N++     D     GDVGSSK      SK SH
Sbjct: 269 GRLQQIRRSISMDHCCQNHTIIADILQLNEDGFGAIDESGSSGDVGSSKHSVGEDSKSSH 328

Query: 701 RNRVLHCVTSPVGMKRSFSSGRFLFPRRGR 790
           R R+LHCV SPV MKRSFSSGRF   R GR
Sbjct: 329 RKRILHCVMSPVSMKRSFSSGRFSLTRHGR 358


>dbj|GAV82564.1| zf-RING_2 domain-containing protein [Cephalotus follicularis]
          Length = 361

 Score =  297 bits (761), Expect = 8e-96
 Identities = 157/270 (58%), Positives = 185/270 (68%), Gaps = 11/270 (4%)
 Frame = +2

Query: 14  SARRRENHDPNVELEENQDPSNHEPWHVANNGLDEALIKSITVCKYKKSDGLIEGTDCSV 193
           ++ RREN DPN E E+NQ+PS HEPWHV   GLD+ALI SITVCKYKK DG IE TDC V
Sbjct: 85  NSTRRENQDPNEESEDNQNPSLHEPWHVLTTGLDDALINSITVCKYKKGDGSIEVTDCPV 144

Query: 194 CLSEFQEDENLRLLPKCSHAFHVKCIDTWLRSHSNCPLCRANIAH--ANALPLQLPPPVT 367
           CLSEFQEDE+LRLLPKCSHAFHV CIDTWL+SHSNCPLCRANI    +N+ P  LPP VT
Sbjct: 145 CLSEFQEDESLRLLPKCSHAFHVPCIDTWLKSHSNCPLCRANIISLGSNSQP-PLPPTVT 203

Query: 368 ETPMSNEIFSHGH--GESTDVDTDVGEEDIMQGDIVPKTPTRVLSDLGNPEQRDTIIEIS 541
            +P   E  S+     +S     D+ EE I   D VPK+P R  SDLGN E R TIIEI 
Sbjct: 204 GSPPGYESLSNNQEPNQSIVASQDIEEETIRHSDGVPKSPLRAFSDLGNLEDRHTIIEIR 263

Query: 542 EVEYQQIRRSASMSHLCQARVSIADVMCINQEE----EDCGMLQGDVGSSKMS---KCSH 700
           E  YQ IRRS SM H CQ R+ +AD++ +NQ+     ++        G+SK +      +
Sbjct: 264 EEGYQSIRRSVSMDHSCQDRLLVADILRMNQDTNVQVDEFHRFTSGAGTSKQTGQFSKPN 323

Query: 701 RNRVLHCVTSPVGMKRSFSSGRFLFPRRGR 790
           + RV+HCV SPV MKRSFSSGRFL  R+GR
Sbjct: 324 KKRVIHCVMSPVAMKRSFSSGRFLLTRQGR 353