BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Acanthopanax21_contig00005398
         (1045 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_021898326.1| taxadiene 5-alpha hydroxylase, partial [Cari...   132   1e-31
gb|PIN05456.1| Cytochrome P450 CYP4/CYP19/CYP26 subfamily [Handr...   114   1e-31
ref|XP_003633223.1| PREDICTED: LOW QUALITY PROTEIN: taxadiene 5-...   115   2e-31
ref|XP_004517250.1| PREDICTED: taxadiene 5-alpha hydroxylase [Ci...   120   4e-31
ref|XP_011098383.1| taxadiene 5-alpha hydroxylase [Sesamum indicum]   132   7e-31
ref|XP_008229454.1| PREDICTED: taxadiene 5-alpha hydroxylase [Pr...   131   1e-30
gb|OVA18932.1| Cytochrome P450 [Macleaya cordata]                     121   1e-30
ref|XP_019459844.1| PREDICTED: taxadiene 5-alpha hydroxylase-lik...   114   2e-30
gb|OIW02853.1| hypothetical protein TanjilG_29629 [Lupinus angus...   114   2e-30
ref|XP_020536245.1| taxadiene 5-alpha hydroxylase [Jatropha curcas]   129   5e-30
ref|XP_003518515.2| PREDICTED: taxadiene 5-alpha hydroxylase [Gl...   122   9e-30
gb|KHN38311.1| Taxadiene 5-alpha hydroxylase [Glycine soja]           109   9e-30
ref|XP_009602928.1| PREDICTED: taxadiene 5-alpha hydroxylase [Ni...   122   1e-29
ref|XP_017429544.1| PREDICTED: taxadiene 5-alpha hydroxylase [Vi...   121   1e-29
ref|XP_023554746.1| taxadiene 5-alpha hydroxylase isoform X2 [Cu...   119   2e-29
ref|XP_022952282.1| taxadiene 5-alpha hydroxylase isoform X2 [Cu...   118   2e-29
ref|XP_016463007.1| PREDICTED: taxadiene 5-alpha hydroxylase-lik...   124   2e-29
ref|XP_009758604.1| PREDICTED: taxadiene 5-alpha hydroxylase-lik...   124   2e-29
ref|XP_016463037.1| PREDICTED: taxadiene 5-alpha hydroxylase-lik...   122   2e-29
ref|XP_007141387.1| hypothetical protein PHAVU_008G191000g [Phas...   128   2e-29

>ref|XP_021898326.1| taxadiene 5-alpha hydroxylase, partial [Carica papaya]
          Length = 377

 Score =  132 bits (332), Expect = 1e-31
 Identities = 75/158 (47%), Positives = 101/158 (63%), Gaps = 12/158 (7%)
 Frame = -3

Query: 869 LFGLLCLYSNVHFDHHVLDTQKKE*PQKPPGEMGLPWIGETIDFNKSQQK-RLFEEFVQP 693
           LF L  + + + F H      +K   + PPGEMGLPWIGET+DF ++Q+  RLF+EF+QP
Sbjct: 14  LFCLAAMVTTILFKHKKNYCIRKS--KLPPGEMGLPWIGETMDFYRAQRNNRLFDEFIQP 71

Query: 692 RITKHGKIFKTSLTGSPT--------ESFL---HVK*VQAGNKLVALFVGAVHGNMEKQG 546
           RI KHG IFKT L GSPT          F+     K V +     ++ +      MEKQG
Sbjct: 72  RIMKHGNIFKTRLMGSPTVVVNGAEANGFILSNEFKLVISSWPSSSVQLMGKESIMEKQG 131

Query: 545 EVHRLLRGVIATSLGPAGLEAIVPKMCNSIQMHLEKNW 432
           E HR +RG+IATSL  +GLE+IVPK+CNS+++HL++NW
Sbjct: 132 EQHRCVRGLIATSLSFSGLESIVPKICNSVKLHLQRNW 169



 Score =  101 bits (251), Expect(2) = 1e-29
 Identities = 63/137 (45%), Positives = 73/137 (53%), Gaps = 6/137 (4%)
 Frame = -1

Query: 643 QQNHFFMSNEFKLVISSWPSSSVQFMGTWK------NKARCTGSFVXXXXXXXXXXXXXX 482
           + N F +SNEFKLVISSWPSSSVQ MG          + RC    +              
Sbjct: 96  EANGFILSNEFKLVISSWPSSSVQLMGKESIMEKQGEQHRCVRGLIATSLSFSGLESIVP 155

Query: 481 XSQKCATQFKCT*KRIGTQLRNN*HLSETISIYGNAKLLTFTIVFECLLGIGVEPGMLEI 302
              K     K         L+ N    ETIS+Y +AK LTF +VFECLLGI VE GML+ 
Sbjct: 156 ---KICNSVKL-------HLQRNWDGHETISLYRSAKSLTFEVVFECLLGIKVEAGMLQT 205

Query: 301 FERVLEGVFTPAISFPG 251
           FERVLEGVF P + FPG
Sbjct: 206 FERVLEGVFAPPVKFPG 222



 Score = 58.5 bits (140), Expect(2) = 1e-29
 Identities = 40/91 (43%), Positives = 47/91 (51%), Gaps = 5/91 (5%)
 Frame = -2

Query: 258 SQATRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXV-----LSRLVAGLIRGXX 94
           S   RAK AR+EIEK+LV  VR K+                      LSR+V GLI+G  
Sbjct: 223 SSFARAKKARQEIEKMLVGVVRDKKKEIMERGKHGYERSNGGKGGMLLSRMVVGLIQGEL 282

Query: 93  XXXXXXXXXXXXVFAAHDTTSFAIAMTFRML 1
                       VFAAHDTTSFAIAMTF++L
Sbjct: 283 SEAEVIDNVVLLVFAAHDTTSFAIAMTFKLL 313


>gb|PIN05456.1| Cytochrome P450 CYP4/CYP19/CYP26 subfamily [Handroanthus
           impetiginosus]
          Length = 439

 Score = 97.4 bits (241), Expect(2) = 1e-31
 Identities = 62/140 (44%), Positives = 77/140 (55%), Gaps = 7/140 (5%)
 Frame = -1

Query: 643 QQNHFFMSNEFKLVISSWPSSSVQFMGTWKNKARCTGSFVXXXXXXXXXXXXXXXSQKCA 464
           + N FF+SNEFKLV+SSWPSSSVQ MG          S +               +   +
Sbjct: 53  EANKFFLSNEFKLVVSSWPSSSVQLMGE--------NSIMEKQGEAHRCLRGIVATSLSS 104

Query: 463 TQFKCT*KRIGTQLRNN*HLSE-------TISIYGNAKLLTFTIVFECLLGIGVEPGMLE 305
           T  +    RI + + N  HL +        I +Y   K+LTFTIV ECLLGI V+ GMLE
Sbjct: 105 TGLENLVPRICSTVEN--HLEKHWNRGEKVILLYSLTKVLTFTIVLECLLGIEVKDGMLE 162

Query: 304 IFERVLEGVFTPAISFPGYK 245
           +FERVLEGVF   I+FPG K
Sbjct: 163 VFERVLEGVFAAPINFPGSK 182



 Score = 68.9 bits (167), Expect(2) = 1e-31
 Identities = 43/86 (50%), Positives = 49/86 (56%)
 Frame = -2

Query: 258 SQATRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXX 79
           S+ +RAK+ARKEIE +LV+  R KR                +LSRLVA LIRG       
Sbjct: 181 SKFSRAKNARKEIENMLVDITREKRKEMEKMEQENGEGEGILLSRLVAALIRGEISEVEV 240

Query: 78  XXXXXXXVFAAHDTTSFAIAMTFRML 1
                  VFAAHDTTSFAIAMT RML
Sbjct: 241 LDNVVLLVFAAHDTTSFAIAMTLRML 266



 Score =  114 bits (285), Expect = 1e-24
 Identities = 62/131 (47%), Positives = 85/131 (64%), Gaps = 12/131 (9%)
 Frame = -3

Query: 773 MGLPWIGETIDFNKSQQK-RLFEEFVQPRITKHGKIFKTSLTGSPT---------ESFL- 627
           MG PWIGETIDF K+Q+K RL+E+F+Q RI KHG+ FKT L G PT         + FL 
Sbjct: 1   MGFPWIGETIDFYKAQKKNRLYEDFIQARIQKHGQTFKTRLMGYPTVVVNGAEANKFFLS 60

Query: 626 -HVK*VQAGNKLVALFVGAVHGNMEKQGEVHRLLRGVIATSLGPAGLEAIVPKMCNSIQM 450
              K V +     ++ +   +  MEKQGE HR LRG++ATSL   GLE +VP++C++++ 
Sbjct: 61  NEFKLVVSSWPSSSVQLMGENSIMEKQGEAHRCLRGIVATSLSSTGLENLVPRICSTVEN 120

Query: 449 HLEKNWYTAQK 417
           HLEK+W   +K
Sbjct: 121 HLEKHWNRGEK 131


>ref|XP_003633223.1| PREDICTED: LOW QUALITY PROTEIN: taxadiene 5-alpha hydroxylase
           [Vitis vinifera]
          Length = 477

 Score =  101 bits (251), Expect(2) = 2e-31
 Identities = 59/131 (45%), Positives = 73/131 (55%)
 Frame = -1

Query: 643 QQNHFFMSNEFKLVISSWPSSSVQFMGTWKNKARCTGSFVXXXXXXXXXXXXXXXSQKCA 464
           + N FF+SNEFKLVISSW S+SVQ MG          S +                    
Sbjct: 96  EANRFFLSNEFKLVISSWTSASVQLMGK--------DSIMEKQGEXRRIIATSLSFAGLE 147

Query: 463 TQFKCT*KRIGTQLRNN*HLSETISIYGNAKLLTFTIVFECLLGIGVEPGMLEIFERVLE 284
           T        +   L    H  +TIS+Y + K+LTFTIVFECLLGI VEP M+++F RVLE
Sbjct: 148 TLVPKICNSVQYHLDTKXHGQDTISLYHSTKVLTFTIVFECLLGINVEPEMIQVFXRVLE 207

Query: 283 GVFTPAISFPG 251
           GVF+P + FPG
Sbjct: 208 GVFSPPVKFPG 218



 Score = 64.7 bits (156), Expect(2) = 2e-31
 Identities = 44/86 (51%), Positives = 49/86 (56%)
 Frame = -2

Query: 258 SQATRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXX 79
           S+ +RAK AR+EIEK+LVE VR+KR                 LSRLVAGLIRG       
Sbjct: 219 SRFSRAKRARQEIEKMLVEIVRKKRQEKKKRVEEGEEGGIL-LSRLVAGLIRGEIKEEVV 277

Query: 78  XXXXXXXVFAAHDTTSFAIAMTFRML 1
                   FAAHDT SF IAMTFRML
Sbjct: 278 DNVVLLV-FAAHDTISFTIAMTFRML 302



 Score =  115 bits (287), Expect = 8e-25
 Identities = 67/127 (52%), Positives = 84/127 (66%), Gaps = 12/127 (9%)
 Frame = -3

Query: 785 PPGEMGLPWIGETIDFNKSQQK-RLFEEFVQPRITKHGKIFKTSLTGSPT---------E 636
           PPGEMGLPWIGET++F K+Q++ RL+EEF+QPRI K+GKIFKTSL GSPT          
Sbjct: 40  PPGEMGLPWIGETVEFYKAQRRYRLYEEFIQPRIAKYGKIFKTSLMGSPTVVVNGEEANR 99

Query: 635 SFL--HVK*VQAGNKLVALFVGAVHGNMEKQGEVHRLLRGVIATSLGPAGLEAIVPKMCN 462
            FL    K V +     ++ +      MEKQGE  R    +IATSL  AGLE +VPK+CN
Sbjct: 100 FFLSNEFKLVISSWTSASVQLMGKDSIMEKQGEXRR----IIATSLSFAGLETLVPKICN 155

Query: 461 SIQMHLE 441
           S+Q HL+
Sbjct: 156 SVQYHLD 162


>ref|XP_004517250.1| PREDICTED: taxadiene 5-alpha hydroxylase [Cicer arietinum]
          Length = 479

 Score =  105 bits (263), Expect(2) = 4e-31
 Identities = 81/220 (36%), Positives = 101/220 (45%), Gaps = 17/220 (7%)
 Frame = -1

Query: 853 VFTPMCILIIMFLIHRKRNSHKSHQ----GKWDFLGLVKQSTLINPNRNGCLRNSFNLVL 686
           V  P+ +LI    +  K+ S+K  +    GK  F  + +     N  R   L   F  V 
Sbjct: 12  VLFPLLLLIFFNFLRHKQQSNKDKRKLPPGKMGFPLIGETMQFFNAQRRNKLYEEF--VH 69

Query: 685 PNMGKSSK-----------QV*RDHQQNHFFMSNEFKLVISSWPSSSVQFMG--TWKNKA 545
           P + K  K            +      N F +SNEFKLV SSWPSSSV  MG  +   K 
Sbjct: 70  PRIAKHGKIFKTRLIGSPTVIVNGADANKFLLSNEFKLVKSSWPSSSVHLMGKDSIMEKD 129

Query: 544 RCTGSFVXXXXXXXXXXXXXXXSQKCATQFKCT*KRIGTQLRNN*HLSETISIYGNAKLL 365
                F+                      F      +   L  N H  E IS+Y + K+L
Sbjct: 130 GERHRFLRGVIGTSLGYAGLESLVPKLCNF------VKLYLVKNWHGQEEISLYKSTKVL 183

Query: 364 TFTIVFECLLGIGVEPGMLEIFERVLEGVFTPAISFPGYK 245
           TF+IVFECLLGI VE GML+ FERVLEGVF+PAI FPG K
Sbjct: 184 TFSIVFECLLGINVEQGMLDTFERVLEGVFSPAIKFPGSK 223



 Score = 58.9 bits (141), Expect(2) = 4e-31
 Identities = 37/86 (43%), Positives = 48/86 (55%)
 Frame = -2

Query: 258 SQATRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXX 79
           S+  RA  ARKEIEK++V+ VR KR                 +S+LV G+I+G       
Sbjct: 222 SKFWRAMKARKEIEKMIVKVVREKRKEMEEGRLEREEDRML-MSKLVYGMIQGEISEKEI 280

Query: 78  XXXXXXXVFAAHDTTSFAIAMTFRML 1
                  VFAAHDTTSFA+AMTF+M+
Sbjct: 281 IDNVVLLVFAAHDTTSFAVAMTFKMM 306



 Score =  120 bits (302), Expect = 7e-27
 Identities = 73/159 (45%), Positives = 94/159 (59%), Gaps = 12/159 (7%)
 Frame = -3

Query: 869 LFGLLCLYSNVHFDHHVLDTQKKE*PQKPPGEMGLPWIGETIDF-NKSQQKRLFEEFVQP 693
           LF LL L       H     + K   + PPG+MG P IGET+ F N  ++ +L+EEFV P
Sbjct: 13  LFPLLLLIFFNFLRHKQQSNKDKR--KLPPGKMGFPLIGETMQFFNAQRRNKLYEEFVHP 70

Query: 692 RITKHGKIFKTSLTGSPT--------ESFL---HVK*VQAGNKLVALFVGAVHGNMEKQG 546
           RI KHGKIFKT L GSPT          FL     K V++     ++ +      MEK G
Sbjct: 71  RIAKHGKIFKTRLIGSPTVIVNGADANKFLLSNEFKLVKSSWPSSSVHLMGKDSIMEKDG 130

Query: 545 EVHRLLRGVIATSLGPAGLEAIVPKMCNSIQMHLEKNWY 429
           E HR LRGVI TSLG AGLE++VPK+CN ++++L KNW+
Sbjct: 131 ERHRFLRGVIGTSLGYAGLESLVPKLCNFVKLYLVKNWH 169


>ref|XP_011098383.1| taxadiene 5-alpha hydroxylase [Sesamum indicum]
          Length = 481

 Score =  132 bits (331), Expect = 7e-31
 Identities = 73/154 (47%), Positives = 97/154 (62%), Gaps = 12/154 (7%)
 Frame = -3

Query: 824 HVLDTQKKE*PQKPPGEMGLPWIGETIDFNKSQQK-RLFEEFVQPRITKHGKIFKTSLTG 648
           H    + K   + PPG+MGLPWIGETIDF K+Q+K RL+E+F+Q RI KHGK FKT L G
Sbjct: 27  HCKRCKNKSKKRLPPGQMGLPWIGETIDFCKAQKKNRLYEDFIQARIQKHGKTFKTRLMG 86

Query: 647 SPT---------ESFL--HVK*VQAGNKLVALFVGAVHGNMEKQGEVHRLLRGVIATSLG 501
           SPT         + FL    K V +     ++ +   +  MEKQGE HR LR ++ATSL 
Sbjct: 87  SPTVVVNGADANKFFLSNEFKLVVSSWPSSSVQLMGENSIMEKQGEAHRCLRAIVATSLS 146

Query: 500 PAGLEAIVPKMCNSIQMHLEKNWYTAQKQLASIR 399
            AG+E +VP+MC +IQMH+EK W   +K ++  R
Sbjct: 147 SAGMEILVPRMCRTIQMHMEKYWNGGEKTISLYR 180



 Score = 97.1 bits (240), Expect(2) = 8e-31
 Identities = 78/229 (34%), Positives = 104/229 (45%), Gaps = 20/229 (8%)
 Frame = -1

Query: 871 FSLAFSVFTPMCILIIMFLIHRKRNSHKSHQG--------KW-----DFLGLVKQSTLIN 731
           F ++ S+   + + + + L H KR  +KS +          W     DF    K++ L  
Sbjct: 7   FLVSVSISALLAVALALVLTHCKRCKNKSKKRLPPGQMGLPWIGETIDFCKAQKKNRLYE 66

Query: 730 PNRNGCLRNSFNLVLPNMGKSSKQV*RDHQQNHFFMSNEFKLVISSWPSSSVQFMGTWK- 554
                 ++         +  S   V      N FF+SNEFKLV+SSWPSSSVQ MG    
Sbjct: 67  DFIQARIQKHGKTFKTRLMGSPTVVVNGADANKFFLSNEFKLVVSSWPSSSVQLMGENSI 126

Query: 553 -----NKARCTGSFVXXXXXXXXXXXXXXXSQKCATQFKCT*KRIGTQLRNN*HLSE-TI 392
                   RC  + V                + C T        I   +    +  E TI
Sbjct: 127 MEKQGEAHRCLRAIVATSLSSAGMEILVP--RMCRT--------IQMHMEKYWNGGEKTI 176

Query: 391 SIYGNAKLLTFTIVFECLLGIGVEPGMLEIFERVLEGVFTPAISFPGYK 245
           S+Y   K+LTFTIV ECLLGI V+PG+LE FERVLEGVF   ++FPG K
Sbjct: 177 SLYRLTKVLTFTIVLECLLGIEVKPGLLETFERVLEGVFAAPVNFPGSK 225



 Score = 66.6 bits (161), Expect(2) = 8e-31
 Identities = 44/86 (51%), Positives = 49/86 (56%)
 Frame = -2

Query: 258 SQATRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXX 79
           S+ +RAKSAR EI  +LVE +R KR+                LSRLVA LIRG       
Sbjct: 224 SKFSRAKSARNEIANMLVEIIRTKRNDMEKQQEKGEEEGIL-LSRLVAALIRGEISEVEV 282

Query: 78  XXXXXXXVFAAHDTTSFAIAMTFRML 1
                  VFAAHDTTSFAIAMTFRML
Sbjct: 283 VDNVVLLVFAAHDTTSFAIAMTFRML 308


>ref|XP_008229454.1| PREDICTED: taxadiene 5-alpha hydroxylase [Prunus mume]
          Length = 481

 Score =  131 bits (329), Expect = 1e-30
 Identities = 77/167 (46%), Positives = 102/167 (61%), Gaps = 12/167 (7%)
 Frame = -3

Query: 896 HQWLCSLVFLFGLLCLYSNVHFDHHVLDTQKKE*PQKPPGEMGLPWIGETIDFNKSQQK- 720
           H   C L  L  ++ +   +    H  +T K+     PPGEMGLPWIG+T++F K+Q+K 
Sbjct: 7   HLCFCFLCILAAMVFIIIFLKHKKHC-NTDKQN---LPPGEMGLPWIGQTMEFYKAQRKN 62

Query: 719 RLFEEFVQPRITKHGKIFKTSLTGSPT--------ESFL---HVK*VQAGNKLVALFVGA 573
           RLF++FVQPRITK+GKIF T L GSPT          FL     K V +     ++ +  
Sbjct: 63  RLFDDFVQPRITKYGKIFSTHLMGSPTVIVNGANANKFLLSNEFKLVVSSWPSASVQLMG 122

Query: 572 VHGNMEKQGEVHRLLRGVIATSLGPAGLEAIVPKMCNSIQMHLEKNW 432
               MEKQGE HR LRG+I +SLG AGLE +VPK+CNS+Q+HL+  W
Sbjct: 123 KDSIMEKQGERHRCLRGLIGSSLGQAGLETLVPKICNSVQLHLDTKW 169



 Score = 88.2 bits (217), Expect(2) = 2e-29
 Identities = 58/146 (39%), Positives = 72/146 (49%), Gaps = 9/146 (6%)
 Frame = -1

Query: 637 NHFFMSNEFKLVISSWPSSSVQFMGTWK------NKARCTGSFVXXXXXXXXXXXXXXXS 476
           N F +SNEFKLV+SSWPS+SVQ MG          + RC    +                
Sbjct: 98  NKFLLSNEFKLVVSSWPSASVQLMGKDSIMEKQGERHRCLRGLIGSSLGQAGLE------ 151

Query: 475 QKCATQFKCT*KRIGTQLRNN*HLSETISIYGNAKLLTFTIVFECLLGIGVEPGMLEIFE 296
               T        +   L       + IS+Y + K+LTFTIVFECLLGI VE  MLE FE
Sbjct: 152 ----TLVPKICNSVQLHLDTKWKGQDKISLYHSTKILTFTIVFECLLGIKVEAEMLETFE 207

Query: 295 RVLEGVFTPAISFPG---YKGKECEK 227
           RVLEGVF   +  PG   ++ K+  K
Sbjct: 208 RVLEGVFAAPVMIPGSSFWRAKKARK 233



 Score = 71.2 bits (173), Expect(2) = 2e-29
 Identities = 44/82 (53%), Positives = 48/82 (58%)
 Frame = -2

Query: 246 RAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXXXXXX 67
           RAK ARK IEK+LVE VR KR                +LSRLVAG+IRG           
Sbjct: 227 RAKKARKTIEKMLVEVVREKRMQMEGKLEEQEEQGGMLLSRLVAGMIRGEITKEEVVDNV 286

Query: 66  XXXVFAAHDTTSFAIAMTFRML 1
              VFAAHDTTSFAIAMTF+ML
Sbjct: 287 VLLVFAAHDTTSFAIAMTFKML 308


>gb|OVA18932.1| Cytochrome P450 [Macleaya cordata]
          Length = 485

 Score =  103 bits (256), Expect(2) = 1e-30
 Identities = 62/139 (44%), Positives = 76/139 (54%), Gaps = 8/139 (5%)
 Frame = -1

Query: 637 NHFFMSNEFKLVISSWPSSSVQFMGTWKNKA--------RCTGSFVXXXXXXXXXXXXXX 482
           N FF+SNEFKLV+SSWPS+SVQ MG  KN          RC  + +              
Sbjct: 105 NRFFLSNEFKLVVSSWPSASVQLMG--KNSIMEKQGDHHRCIRAVIAASLSCSGLE---- 158

Query: 481 XSQKCATQFKCT*KRIGTQLRNN*HLSETISIYGNAKLLTFTIVFECLLGIGVEPGMLEI 302
                 T        +   L  +    ETI ++ + K+LTF+IVFECL GI VEPGMLE+
Sbjct: 159 ------TLVPKICNSVEVHLNTHWQGQETIGLFRSTKVLTFSIVFECLFGIKVEPGMLEM 212

Query: 301 FERVLEGVFTPAISFPGYK 245
           FERVLEGVF P + FPG K
Sbjct: 213 FERVLEGVFAPPVEFPGSK 231



 Score = 59.7 bits (143), Expect(2) = 1e-30
 Identities = 42/86 (48%), Positives = 45/86 (52%)
 Frame = -2

Query: 258 SQATRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXX 79
           S+  RAK AR EIE++LV  VR KR                 LSRLVA LIRG       
Sbjct: 230 SKFWRAKKARMEIERMLVNIVRNKRKEMEMEGEEEDGML---LSRLVASLIRGEIKEEEV 286

Query: 78  XXXXXXXVFAAHDTTSFAIAMTFRML 1
                  VFAAHDTTSFAIAM  RML
Sbjct: 287 LDNIVLLVFAAHDTTSFAIAMVCRML 312



 Score =  121 bits (303), Expect = 5e-27
 Identities = 70/158 (44%), Positives = 97/158 (61%), Gaps = 15/158 (9%)
 Frame = -3

Query: 860 LLCLYSNVHFDHHVLDTQKKE*PQK---PPGEMGLPWIGETIDFNKSQQK-RLFEEFVQP 693
           LL   S +H + +  +   K   +K   PPG++GLPWIGET++F ++Q+  R+FEEFVQP
Sbjct: 19  LLYFISLIHKNKNNNNNNNKNRSKKKKLPPGDLGLPWIGETLEFYRAQRNNRIFEEFVQP 78

Query: 692 RITKHGKIFKTSLTGSPT---------ESFL--HVK*VQAGNKLVALFVGAVHGNMEKQG 546
           R TKHG IFKT L GSPT           FL    K V +     ++ +   +  MEKQG
Sbjct: 79  RTTKHGTIFKTRLIGSPTVVVSGAAANRFFLSNEFKLVVSSWPSASVQLMGKNSIMEKQG 138

Query: 545 EVHRLLRGVIATSLGPAGLEAIVPKMCNSIQMHLEKNW 432
           + HR +R VIA SL  +GLE +VPK+CNS+++HL  +W
Sbjct: 139 DHHRCIRAVIAASLSCSGLETLVPKICNSVEVHLNTHW 176


>ref|XP_019459844.1| PREDICTED: taxadiene 5-alpha hydroxylase-like [Lupinus
           angustifolius]
          Length = 490

 Score =  102 bits (253), Expect(2) = 2e-30
 Identities = 79/229 (34%), Positives = 107/229 (46%), Gaps = 20/229 (8%)
 Frame = -1

Query: 871 FSLAFSVFTPMCILIIMFLIHRK---RNSHKSHQGKWDFLGLVKQSTLINPNRNGCLRNS 701
           + L +++F  + ++   FL H+K   R+      G      + +     N  R   L   
Sbjct: 19  YVLLWTLFPLIAVVYFSFLRHKKCCNRDKKVLPPGDMGLPFIGETMEFFNAQRRNQLFEE 78

Query: 700 FNLVLPNMGKSSK-----------QV*RDHQQNHFFMSNEFKLVISSWPSSSVQFMGTW- 557
           F  V P + K  K            V    + N F +SNEFKLV SSWPSSSVQ MG+  
Sbjct: 79  F--VEPRIQKHGKIFKTRIMGSPTVVVNGAEANKFILSNEFKLVKSSWPSSSVQLMGSDC 136

Query: 556 -----KNKARCTGSFVXXXXXXXXXXXXXXXSQKCATQFKCT*KRIGTQLRNN*HLSETI 392
                 +K R     +                  C++        +   L  N    E +
Sbjct: 137 IMEKDSDKHRYLRGVIGTTLGYAGLEVLVPKI--CSS--------VRFYLATNWKGQEKV 186

Query: 391 SIYGNAKLLTFTIVFECLLGIGVEPGMLEIFERVLEGVFTPAISFPGYK 245
           S+Y + K+LTF+IVFECLLGI VEPGML+ FERVLEGVF+PA+ FPG K
Sbjct: 187 SLYHSTKVLTFSIVFECLLGINVEPGMLDTFERVLEGVFSPAVEFPGSK 235



 Score = 60.5 bits (145), Expect(2) = 2e-30
 Identities = 40/86 (46%), Positives = 48/86 (55%)
 Frame = -2

Query: 258 SQATRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXX 79
           S+  RAK AR EIEK+L++ VR+KR                 LSRLV G+I+G       
Sbjct: 234 SKFWRAKKARVEIEKMLIKVVRKKRKEMEEKLLSKENGML--LSRLVHGMIQGEISEKEV 291

Query: 78  XXXXXXXVFAAHDTTSFAIAMTFRML 1
                  VFAAHDTTS AIAMTF+ML
Sbjct: 292 IDNVVLLVFAAHDTTSIAIAMTFKML 317



 Score =  114 bits (285), Expect = 2e-24
 Identities = 63/138 (45%), Positives = 87/138 (63%), Gaps = 15/138 (10%)
 Frame = -3

Query: 785 PPGEMGLPWIGETIDF-NKSQQKRLFEEFVQPRITKHGKIFKTSLTGSPTESFLHVK*VQ 609
           PPG+MGLP+IGET++F N  ++ +LFEEFV+PRI KHGKIFKT + GSPT   + V   +
Sbjct: 51  PPGDMGLPFIGETMEFFNAQRRNQLFEEFVEPRIQKHGKIFKTRIMGSPT---VVVNGAE 107

Query: 608 AGNKLVALFVGAVHGN--------------MEKQGEVHRLLRGVIATSLGPAGLEAIVPK 471
           A   +++     V  +              MEK  + HR LRGVI T+LG AGLE +VPK
Sbjct: 108 ANKFILSNEFKLVKSSWPSSSVQLMGSDCIMEKDSDKHRYLRGVIGTTLGYAGLEVLVPK 167

Query: 470 MCNSIQMHLEKNWYTAQK 417
           +C+S++ +L  NW   +K
Sbjct: 168 ICSSVRFYLATNWKGQEK 185


>gb|OIW02853.1| hypothetical protein TanjilG_29629 [Lupinus angustifolius]
          Length = 478

 Score =  102 bits (253), Expect(2) = 2e-30
 Identities = 79/229 (34%), Positives = 107/229 (46%), Gaps = 20/229 (8%)
 Frame = -1

Query: 871 FSLAFSVFTPMCILIIMFLIHRK---RNSHKSHQGKWDFLGLVKQSTLINPNRNGCLRNS 701
           + L +++F  + ++   FL H+K   R+      G      + +     N  R   L   
Sbjct: 7   YVLLWTLFPLIAVVYFSFLRHKKCCNRDKKVLPPGDMGLPFIGETMEFFNAQRRNQLFEE 66

Query: 700 FNLVLPNMGKSSK-----------QV*RDHQQNHFFMSNEFKLVISSWPSSSVQFMGTW- 557
           F  V P + K  K            V    + N F +SNEFKLV SSWPSSSVQ MG+  
Sbjct: 67  F--VEPRIQKHGKIFKTRIMGSPTVVVNGAEANKFILSNEFKLVKSSWPSSSVQLMGSDC 124

Query: 556 -----KNKARCTGSFVXXXXXXXXXXXXXXXSQKCATQFKCT*KRIGTQLRNN*HLSETI 392
                 +K R     +                  C++        +   L  N    E +
Sbjct: 125 IMEKDSDKHRYLRGVIGTTLGYAGLEVLVPKI--CSS--------VRFYLATNWKGQEKV 174

Query: 391 SIYGNAKLLTFTIVFECLLGIGVEPGMLEIFERVLEGVFTPAISFPGYK 245
           S+Y + K+LTF+IVFECLLGI VEPGML+ FERVLEGVF+PA+ FPG K
Sbjct: 175 SLYHSTKVLTFSIVFECLLGINVEPGMLDTFERVLEGVFSPAVEFPGSK 223



 Score = 60.5 bits (145), Expect(2) = 2e-30
 Identities = 40/86 (46%), Positives = 48/86 (55%)
 Frame = -2

Query: 258 SQATRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXX 79
           S+  RAK AR EIEK+L++ VR+KR                 LSRLV G+I+G       
Sbjct: 222 SKFWRAKKARVEIEKMLIKVVRKKRKEMEEKLLSKENGML--LSRLVHGMIQGEISEKEV 279

Query: 78  XXXXXXXVFAAHDTTSFAIAMTFRML 1
                  VFAAHDTTS AIAMTF+ML
Sbjct: 280 IDNVVLLVFAAHDTTSIAIAMTFKML 305



 Score =  114 bits (285), Expect = 1e-24
 Identities = 63/138 (45%), Positives = 87/138 (63%), Gaps = 15/138 (10%)
 Frame = -3

Query: 785 PPGEMGLPWIGETIDF-NKSQQKRLFEEFVQPRITKHGKIFKTSLTGSPTESFLHVK*VQ 609
           PPG+MGLP+IGET++F N  ++ +LFEEFV+PRI KHGKIFKT + GSPT   + V   +
Sbjct: 39  PPGDMGLPFIGETMEFFNAQRRNQLFEEFVEPRIQKHGKIFKTRIMGSPT---VVVNGAE 95

Query: 608 AGNKLVALFVGAVHGN--------------MEKQGEVHRLLRGVIATSLGPAGLEAIVPK 471
           A   +++     V  +              MEK  + HR LRGVI T+LG AGLE +VPK
Sbjct: 96  ANKFILSNEFKLVKSSWPSSSVQLMGSDCIMEKDSDKHRYLRGVIGTTLGYAGLEVLVPK 155

Query: 470 MCNSIQMHLEKNWYTAQK 417
           +C+S++ +L  NW   +K
Sbjct: 156 ICSSVRFYLATNWKGQEK 173


>ref|XP_020536245.1| taxadiene 5-alpha hydroxylase [Jatropha curcas]
          Length = 484

 Score =  129 bits (325), Expect = 5e-30
 Identities = 79/172 (45%), Positives = 108/172 (62%), Gaps = 12/172 (6%)
 Frame = -3

Query: 881 SLVFLFGLLCLYSNVHFDHHVLDTQKKE*PQKPPGEMGLPWIGETIDFNKSQQK-RLFEE 705
           S VF   LL L     F ++   +++K     PPG+MG PWIGET++F KSQ+K RLFE+
Sbjct: 9   SWVFFSLLLSLILYTIFLNYNSQSKRK----LPPGKMGFPWIGETMEFFKSQRKNRLFED 64

Query: 704 FVQPRITKHGKIFKTSLTGSPT---------ESFL--HVK*VQAGNKLVALFVGAVHGNM 558
           FV+PRITK+GKIFKT L G PT         + FL    K V +     ++ +   +  M
Sbjct: 65  FVEPRITKYGKIFKTRLMGWPTIVVNGAEANKFFLANEFKVVVSSWPSTSVELMGKNSIM 124

Query: 557 EKQGEVHRLLRGVIATSLGPAGLEAIVPKMCNSIQMHLEKNWYTAQKQLASI 402
           EKQG+ HR LRG IA+SL  AGLEA+VPK+CNS+++HL+K W   +  + +I
Sbjct: 125 EKQGDRHRCLRGFIASSLNSAGLEALVPKICNSVELHLQKYWNNNKNSINTI 176



 Score =  100 bits (249), Expect(2) = 9e-30
 Identities = 79/238 (33%), Positives = 108/238 (45%), Gaps = 22/238 (9%)
 Frame = -1

Query: 874 FFSLAFSVFTPMCILIIMFLIHRKRNSHKSHQGKWDFLGLVKQSTLINPNRNGCLRNSFN 695
           FFSL  S+     IL  +FL +  ++  K   GK  F  + +        R   L   F 
Sbjct: 12  FFSLLLSL-----ILYTIFLNYNSQSKRKLPPGKMGFPWIGETMEFFKSQRKNRLFEDF- 65

Query: 694 LVLPNMGKSSKQ-----------V*RDHQQNHFFMSNEFKLVISSWPSSSVQFMGTWK-- 554
            V P + K  K            V    + N FF++NEFK+V+SSWPS+SV+ MG     
Sbjct: 66  -VEPRITKYGKIFKTRLMGWPTIVVNGAEANKFFLANEFKVVVSSWPSTSVELMGKNSIM 124

Query: 553 ----NKARCTGSFVXXXXXXXXXXXXXXXSQKCATQFKCT*KRIGTQL--RNN*HLSETI 392
               ++ RC   F+                        C    +  Q    NN +   TI
Sbjct: 125 EKQGDRHRCLRGFIASSLNSAGLEALVPKI--------CNSVELHLQKYWNNNKNSINTI 176

Query: 391 SIYGNAKLLTFTIVFECLLGIGVEPGMLEIFERVLEGVFTPAISFPG---YKGKECEK 227
           S+Y + K+LTFTIVFECLLGI    G+   FE++L+GVF  A+SFPG   YK K+  K
Sbjct: 177 SLYNSTKVLTFTIVFECLLGIKANSGIFTTFEKILDGVFALALSFPGFRFYKAKKARK 234



 Score = 59.7 bits (143), Expect(2) = 9e-30
 Identities = 38/82 (46%), Positives = 44/82 (53%)
 Frame = -2

Query: 246 RAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXXXXXX 67
           +AK ARK +EK LVE VR KR                 L +L+ G+IRG           
Sbjct: 228 KAKKARKAVEKFLVEVVREKRKKMEEGLEKEEEGML--LDQLLRGMIRGEISEEEVIDNV 285

Query: 66  XXXVFAAHDTTSFAIAMTFRML 1
              VFAAHDTTSFAIAMTF+ML
Sbjct: 286 VLLVFAAHDTTSFAIAMTFKML 307


>ref|XP_003518515.2| PREDICTED: taxadiene 5-alpha hydroxylase [Glycine max]
 gb|KRH73677.1| hypothetical protein GLYMA_02G287300 [Glycine max]
          Length = 493

 Score = 98.2 bits (243), Expect(2) = 9e-30
 Identities = 60/133 (45%), Positives = 74/133 (55%)
 Frame = -1

Query: 643 QQNHFFMSNEFKLVISSWPSSSVQFMGTWKNKARCTGSFVXXXXXXXXXXXXXXXSQKCA 464
           + N F +SNEFKLV SSWPSSSV+ MG      +  G                    +  
Sbjct: 110 EANKFLLSNEFKLVKSSWPSSSVELMGRDSIMEKDGGRH--RFLRGVIGTSLGYAGLELL 167

Query: 463 TQFKCT*KRIGTQLRNN*HLSETISIYGNAKLLTFTIVFECLLGIGVEPGMLEIFERVLE 284
               C    +   L  N    E IS+Y + K+L+F+IVFECLLGI VEPGML+ FERVLE
Sbjct: 168 VPKLCN--SVQFHLATNWKGQEKISLYRSTKVLSFSIVFECLLGIKVEPGMLDTFERVLE 225

Query: 283 GVFTPAISFPGYK 245
           GVF+PA+ FPG K
Sbjct: 226 GVFSPAVMFPGSK 238



 Score = 62.0 bits (149), Expect(2) = 9e-30
 Identities = 40/86 (46%), Positives = 50/86 (58%)
 Frame = -2

Query: 258 SQATRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXX 79
           S+  RAK AR EIEK+LV+ VR KR                +LS+LV+G+I+G       
Sbjct: 237 SKFWRAKKARVEIEKMLVKVVREKR--REMEGSLGREQDGMLLSKLVSGMIQGEISEKEV 294

Query: 78  XXXXXXXVFAAHDTTSFAIAMTFRML 1
                  VFAAHDTTSFA+AMTF+ML
Sbjct: 295 IDNVVLLVFAAHDTTSFAVAMTFKML 320



 Score =  122 bits (307), Expect = 2e-27
 Identities = 75/163 (46%), Positives = 95/163 (58%), Gaps = 12/163 (7%)
 Frame = -3

Query: 869 LFGLLCLYSNVHFDHHVLDTQKKE*PQKPPGEMGLPWIGETIDF-NKSQQKRLFEEFVQP 693
           LF LL L  + +F  H    + K     PPGEMG P IGET++F N  ++ +LFEEFV P
Sbjct: 28  LFSLLLLLVSFNFLRHKQCYKDKR--NLPPGEMGFPLIGETMEFFNAQRRNQLFEEFVHP 85

Query: 692 RITKHGKIFKTSLTGSPT--------ESFL---HVK*VQAGNKLVALFVGAVHGNMEKQG 546
           RI KHG+IF+T + GSPT          FL     K V++     ++ +      MEK G
Sbjct: 86  RILKHGRIFRTRIMGSPTVVVNGAEANKFLLSNEFKLVKSSWPSSSVELMGRDSIMEKDG 145

Query: 545 EVHRLLRGVIATSLGPAGLEAIVPKMCNSIQMHLEKNWYTAQK 417
             HR LRGVI TSLG AGLE +VPK+CNS+Q HL  NW   +K
Sbjct: 146 GRHRFLRGVIGTSLGYAGLELLVPKLCNSVQFHLATNWKGQEK 188


>gb|KHN38311.1| Taxadiene 5-alpha hydroxylase [Glycine soja]
          Length = 436

 Score = 98.2 bits (243), Expect(2) = 9e-30
 Identities = 60/133 (45%), Positives = 74/133 (55%)
 Frame = -1

Query: 643 QQNHFFMSNEFKLVISSWPSSSVQFMGTWKNKARCTGSFVXXXXXXXXXXXXXXXSQKCA 464
           + N F +SNEFKLV SSWPSSSV+ MG      +  G                    +  
Sbjct: 53  EANKFLLSNEFKLVKSSWPSSSVELMGRDSIMEKDGGRH--RFLRGVIGTSLGYAGLELL 110

Query: 463 TQFKCT*KRIGTQLRNN*HLSETISIYGNAKLLTFTIVFECLLGIGVEPGMLEIFERVLE 284
               C    +   L  N    E IS+Y + K+L+F+IVFECLLGI VEPGML+ FERVLE
Sbjct: 111 VPKLCN--SVQFHLATNWKGQEKISLYRSTKVLSFSIVFECLLGIKVEPGMLDTFERVLE 168

Query: 283 GVFTPAISFPGYK 245
           GVF+PA+ FPG K
Sbjct: 169 GVFSPAVMFPGSK 181



 Score = 62.0 bits (149), Expect(2) = 9e-30
 Identities = 40/86 (46%), Positives = 50/86 (58%)
 Frame = -2

Query: 258 SQATRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXX 79
           S+  RAK AR EIEK+LV+ VR KR                +LS+LV+G+I+G       
Sbjct: 180 SKFWRAKKARVEIEKMLVKVVREKR--REMEGSLGREQDGMLLSKLVSGMIQGEISEKEV 237

Query: 78  XXXXXXXVFAAHDTTSFAIAMTFRML 1
                  VFAAHDTTSFA+AMTF+ML
Sbjct: 238 IDNVVLLVFAAHDTTSFAVAMTFKML 263



 Score =  109 bits (272), Expect = 6e-23
 Identities = 63/131 (48%), Positives = 80/131 (61%), Gaps = 12/131 (9%)
 Frame = -3

Query: 773 MGLPWIGETIDF-NKSQQKRLFEEFVQPRITKHGKIFKTSLTGSPT--------ESFL-- 627
           MG P IGET++F N  ++ +LFEEFV PRI KHG+IF+T + GSPT          FL  
Sbjct: 1   MGFPLIGETMEFFNAQRRNQLFEEFVHPRILKHGRIFRTRIMGSPTVVVNGAEANKFLLS 60

Query: 626 -HVK*VQAGNKLVALFVGAVHGNMEKQGEVHRLLRGVIATSLGPAGLEAIVPKMCNSIQM 450
              K V++     ++ +      MEK G  HR LRGVI TSLG AGLE +VPK+CNS+Q 
Sbjct: 61  NEFKLVKSSWPSSSVELMGRDSIMEKDGGRHRFLRGVIGTSLGYAGLELLVPKLCNSVQF 120

Query: 449 HLEKNWYTAQK 417
           HL  NW   +K
Sbjct: 121 HLATNWKGQEK 131


>ref|XP_009602928.1| PREDICTED: taxadiene 5-alpha hydroxylase [Nicotiana
           tomentosiformis]
          Length = 485

 Score = 89.7 bits (221), Expect(2) = 1e-29
 Identities = 58/139 (41%), Positives = 69/139 (49%), Gaps = 6/139 (4%)
 Frame = -1

Query: 643 QQNHFFMSNEFKLVISSWPSSSVQFMGT------WKNKARCTGSFVXXXXXXXXXXXXXX 482
           + N FF+SNEFKLVISSWP+SSV+ MG         +  RC    +              
Sbjct: 100 EANMFFLSNEFKLVISSWPTSSVELMGKNCIMEKQGDAHRCLRGIISSSLASFSLDAMVP 159

Query: 481 XSQKCATQFKCT*KRIGTQLRNN*HLSETISIYGNAKLLTFTIVFECLLGIGVEPGMLEI 302
               C T      K    Q        + I +Y   K LTF IVFEC LGI V+PG+LE 
Sbjct: 160 KI--CNTIQSHLDKNCNGQ-------DQIIKLYHLTKYLTFKIVFECFLGIVVKPGLLET 210

Query: 301 FERVLEGVFTPAISFPGYK 245
           FERVLEG F+P   FPG K
Sbjct: 211 FERVLEGAFSPPFKFPGSK 229



 Score = 70.1 bits (170), Expect(2) = 1e-29
 Identities = 44/86 (51%), Positives = 50/86 (58%)
 Frame = -2

Query: 258 SQATRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXX 79
           S+ +RAK+AR +IEKILVE +R KR                +LSRLV  LIRG       
Sbjct: 228 SKFSRAKNARLKIEKILVEVIREKRREIELGEEKEGKLDEALLSRLVKALIRGEVSEEEV 287

Query: 78  XXXXXXXVFAAHDTTSFAIAMTFRML 1
                  VFAAHDTTSFAIAMTFRML
Sbjct: 288 VDNVVLLVFAAHDTTSFAIAMTFRML 313



 Score =  122 bits (306), Expect = 2e-27
 Identities = 73/146 (50%), Positives = 95/146 (65%), Gaps = 12/146 (8%)
 Frame = -3

Query: 836 HFDHHVLDTQKKE*PQKPPGEMGLPWIGETIDFNKSQQK-RLFEEFVQPRITKHGKIFKT 660
           H+  +   T+KK     PPGEMGLPWIGETI+F K+Q+K +LFEEFVQPRI K+GK FKT
Sbjct: 31  HYKGYKHITKKK----LPPGEMGLPWIGETIEFYKAQKKNKLFEEFVQPRIEKYGKTFKT 86

Query: 659 SLTGSPT---------ESFL--HVK*VQAGNKLVALFVGAVHGNMEKQGEVHRLLRGVIA 513
            L GSPT           FL    K V +     ++ +   +  MEKQG+ HR LRG+I+
Sbjct: 87  RLMGSPTVVVCGAEANMFFLSNEFKLVISSWPTSSVELMGKNCIMEKQGDAHRCLRGIIS 146

Query: 512 TSLGPAGLEAIVPKMCNSIQMHLEKN 435
           +SL    L+A+VPK+CN+IQ HL+KN
Sbjct: 147 SSLASFSLDAMVPKICNTIQSHLDKN 172


>ref|XP_017429544.1| PREDICTED: taxadiene 5-alpha hydroxylase [Vigna angularis]
 ref|XP_017429545.1| PREDICTED: taxadiene 5-alpha hydroxylase [Vigna angularis]
 dbj|BAT80757.1| hypothetical protein VIGAN_03035600 [Vigna angularis var.
           angularis]
          Length = 478

 Score =  100 bits (248), Expect(2) = 1e-29
 Identities = 78/219 (35%), Positives = 102/219 (46%), Gaps = 19/219 (8%)
 Frame = -1

Query: 844 PMCILIIMFLIHRK----RNSHKSHQGKWDFLGLVKQSTLINPNR-NGCLRNSFNLVLPN 680
           P+ +L++ F+  R     ++  K   G+  F  + +     N  R N      F+  +  
Sbjct: 15  PLLLLLVSFIFLRHEQCCKDKRKLPPGEMGFPLIGETMEFFNAQRRNKLFEEFFHPRILK 74

Query: 679 MGK--------SSKQV*RDHQQNHFFMSNEFKLVISSWPSSSVQFMGTWK------NKAR 542
            GK        S   V    + N F +SNEFKLV SSWPSSSV+ MG          + R
Sbjct: 75  HGKIFRTRIMGSPTVVVNGAEANKFLLSNEFKLVKSSWPSSSVELMGRDSIMEKDGERHR 134

Query: 541 CTGSFVXXXXXXXXXXXXXXXSQKCATQFKCT*KRIGTQLRNN*HLSETISIYGNAKLLT 362
                +                  C  QF          L  N    E ISI+ + K+LT
Sbjct: 135 FLRGVIATSLGYAGLELLVPRLCNCV-QF---------HLATNWKGQENISIHRSTKVLT 184

Query: 361 FTIVFECLLGIGVEPGMLEIFERVLEGVFTPAISFPGYK 245
           F+IVFECLLGI VEPGML+ FERVLEGVF+PA+ FPG K
Sbjct: 185 FSIVFECLLGIKVEPGMLDTFERVLEGVFSPAVMFPGSK 223



 Score = 59.7 bits (143), Expect(2) = 1e-29
 Identities = 39/86 (45%), Positives = 47/86 (54%)
 Frame = -2

Query: 258 SQATRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXX 79
           S+  RAK AR EIEK+L++ VR KR                 LS LV G+I+G       
Sbjct: 222 SKFWRAKKARVEIEKMLLKVVREKRKEMEGSSEREQDGML--LSNLVCGMIQGEISEKEV 279

Query: 78  XXXXXXXVFAAHDTTSFAIAMTFRML 1
                  VFAAHDTTSFA+AMTF+ML
Sbjct: 280 IDNVVLLVFAAHDTTSFAVAMTFKML 305



 Score =  121 bits (303), Expect = 5e-27
 Identities = 74/158 (46%), Positives = 93/158 (58%), Gaps = 12/158 (7%)
 Frame = -3

Query: 869 LFGLLCLYSNVHFDHHVLDTQKKE*PQKPPGEMGLPWIGETIDF-NKSQQKRLFEEFVQP 693
           LF LL L  +  F  H    + K   + PPGEMG P IGET++F N  ++ +LFEEF  P
Sbjct: 13  LFPLLLLLVSFIFLRHEQCCKDKR--KLPPGEMGFPLIGETMEFFNAQRRNKLFEEFFHP 70

Query: 692 RITKHGKIFKTSLTGSPT--------ESFL---HVK*VQAGNKLVALFVGAVHGNMEKQG 546
           RI KHGKIF+T + GSPT          FL     K V++     ++ +      MEK G
Sbjct: 71  RILKHGKIFRTRIMGSPTVVVNGAEANKFLLSNEFKLVKSSWPSSSVELMGRDSIMEKDG 130

Query: 545 EVHRLLRGVIATSLGPAGLEAIVPKMCNSIQMHLEKNW 432
           E HR LRGVIATSLG AGLE +VP++CN +Q HL  NW
Sbjct: 131 ERHRFLRGVIATSLGYAGLELLVPRLCNCVQFHLATNW 168


>ref|XP_023554746.1| taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita pepo subsp.
           pepo]
          Length = 486

 Score =  101 bits (252), Expect(2) = 2e-29
 Identities = 79/229 (34%), Positives = 103/229 (44%), Gaps = 18/229 (7%)
 Frame = -1

Query: 859 FSVFTPMCILIIMFLIHRKRNSHKSHQGKWDFLGLVKQSTLINPNRNGCLRNSFNLVLPN 680
           F   + + I+ I+F+    + S K   G+     + +        R   L   F  V P 
Sbjct: 14  FLFLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEF--VGPR 71

Query: 679 MGKSSK-----------QV*RDHQQNHFFMSNEFKLVISSWPSSSVQFMGT------WKN 551
           + K  K            V    + N FF+SNEFKLV+SSWPS+SVQ MG          
Sbjct: 72  VAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGE 131

Query: 550 KARCTGSFVXXXXXXXXXXXXXXXSQKCAT-QFKCT*KRIGTQLRNN*HLSETISIYGNA 374
             RC    +                + C T Q     K +G  +         IS+Y +A
Sbjct: 132 SHRCVRGLIAASLASSSLEAMMP--KLCTTIQLYLDTKWLGHDI---------ISLYHSA 180

Query: 373 KLLTFTIVFECLLGIGVEPGMLEIFERVLEGVFTPAISFPGYKGKECEK 227
           K+LTFTIVFECL+GI VEP ML  FERVLEGVF P  SFPG +    +K
Sbjct: 181 KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKK 229



 Score = 57.8 bits (138), Expect(2) = 2e-29
 Identities = 39/83 (46%), Positives = 45/83 (54%)
 Frame = -2

Query: 249 TRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXXXXX 70
           +RAK AR EI K+L E VR KR                +LSR+V  +IRG          
Sbjct: 225 SRAKKARMEIGKMLREVVREKR--RRIEEEPEEEECELLLSRMVRAMIRGEISEVEVVDN 282

Query: 69  XXXXVFAAHDTTSFAIAMTFRML 1
               VFAAHDTTSFAIAMTF+ML
Sbjct: 283 VVLLVFAAHDTTSFAIAMTFKML 305



 Score =  119 bits (297), Expect = 4e-26
 Identities = 72/165 (43%), Positives = 100/165 (60%), Gaps = 12/165 (7%)
 Frame = -3

Query: 890 WLCSLVFLFGLLCLYSNVHFDHHVLDTQKKE*PQKPPGEMGLPWIGETIDFNKSQQK-RL 714
           +L + +FLF    L  ++ F    +   KK     PPG+MGLPWIGET+ F ++Q+K RL
Sbjct: 8   FLLATMFLFLSAILIISIIFVFRSIKGSKKV----PPGQMGLPWIGETMAFYRAQRKNRL 63

Query: 713 FEEFVQPRITKHGKIFKTSLTGSPT---------ESFL--HVK*VQAGNKLVALFVGAVH 567
           FEEFV PR+ K+GKIFKTSL GSPT           FL    K V +     ++ +    
Sbjct: 64  FEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEE 123

Query: 566 GNMEKQGEVHRLLRGVIATSLGPAGLEAIVPKMCNSIQMHLEKNW 432
             M+KQGE HR +RG+IA SL  + LEA++PK+C +IQ++L+  W
Sbjct: 124 CIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKW 168


>ref|XP_022952282.1| taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita moschata]
          Length = 486

 Score =  101 bits (252), Expect(2) = 2e-29
 Identities = 79/229 (34%), Positives = 103/229 (44%), Gaps = 18/229 (7%)
 Frame = -1

Query: 859 FSVFTPMCILIIMFLIHRKRNSHKSHQGKWDFLGLVKQSTLINPNRNGCLRNSFNLVLPN 680
           F   + + I+ I+F+    + S K   G+     + +        R   L   F  V P 
Sbjct: 14  FLFLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGETMVFYRAQRKNRLFEEF--VGPR 71

Query: 679 MGKSSK-----------QV*RDHQQNHFFMSNEFKLVISSWPSSSVQFMGT------WKN 551
           + K  K            V    + N FF+SNEFKLV+SSWPS+SVQ MG          
Sbjct: 72  VAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGE 131

Query: 550 KARCTGSFVXXXXXXXXXXXXXXXSQKCAT-QFKCT*KRIGTQLRNN*HLSETISIYGNA 374
             RC    +                + C T Q     K +G  +         IS+Y +A
Sbjct: 132 SHRCVRGLIAASLASSSLEAMMP--KLCTTIQLYLDTKWLGHDI---------ISLYHSA 180

Query: 373 KLLTFTIVFECLLGIGVEPGMLEIFERVLEGVFTPAISFPGYKGKECEK 227
           K+LTFTIVFECL+GI VEP ML  FERVLEGVF P  SFPG +    +K
Sbjct: 181 KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKK 229



 Score = 57.8 bits (138), Expect(2) = 2e-29
 Identities = 39/83 (46%), Positives = 45/83 (54%)
 Frame = -2

Query: 249 TRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXXXXX 70
           +RAK AR EI K+L E VR KR                +LSR+V  +IRG          
Sbjct: 225 SRAKKARMEIGKMLREVVREKR--RRIEEEPVEEECELLLSRMVRAMIRGEISEVEVVDN 282

Query: 69  XXXXVFAAHDTTSFAIAMTFRML 1
               VFAAHDTTSFAIAMTF+ML
Sbjct: 283 VVLLVFAAHDTTSFAIAMTFKML 305



 Score =  118 bits (296), Expect = 5e-26
 Identities = 72/165 (43%), Positives = 100/165 (60%), Gaps = 12/165 (7%)
 Frame = -3

Query: 890 WLCSLVFLFGLLCLYSNVHFDHHVLDTQKKE*PQKPPGEMGLPWIGETIDFNKSQQK-RL 714
           +L + +FLF    L  ++ F    +   KK     PPG+MGLPWIGET+ F ++Q+K RL
Sbjct: 8   FLLATMFLFLSAILIISIIFVFRSIKGSKKV----PPGQMGLPWIGETMVFYRAQRKNRL 63

Query: 713 FEEFVQPRITKHGKIFKTSLTGSPT---------ESFL--HVK*VQAGNKLVALFVGAVH 567
           FEEFV PR+ K+GKIFKTSL GSPT           FL    K V +     ++ +    
Sbjct: 64  FEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEE 123

Query: 566 GNMEKQGEVHRLLRGVIATSLGPAGLEAIVPKMCNSIQMHLEKNW 432
             M+KQGE HR +RG+IA SL  + LEA++PK+C +IQ++L+  W
Sbjct: 124 CIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKW 168


>ref|XP_016463007.1| PREDICTED: taxadiene 5-alpha hydroxylase-like [Nicotiana tabacum]
          Length = 485

 Score = 89.4 bits (220), Expect(2) = 2e-29
 Identities = 58/139 (41%), Positives = 69/139 (49%), Gaps = 6/139 (4%)
 Frame = -1

Query: 643 QQNHFFMSNEFKLVISSWPSSSVQFMGT------WKNKARCTGSFVXXXXXXXXXXXXXX 482
           + N FF+SNEFKLVISSWP+SSV+ MG         +  RC    +              
Sbjct: 100 EANMFFLSNEFKLVISSWPTSSVELMGKNCIMEKQGDAHRCLRGIISSSLASFSLDAMVP 159

Query: 481 XSQKCATQFKCT*KRIGTQLRNN*HLSETISIYGNAKLLTFTIVFECLLGIGVEPGMLEI 302
               C T      K    Q        + I +Y   K LTF IVFEC LGI V+PG+LE 
Sbjct: 160 KI--CNTIQSHLDKNCNGQ-------DQIIKLYHLTKSLTFKIVFECFLGIVVKPGLLET 210

Query: 301 FERVLEGVFTPAISFPGYK 245
           FERVLEG F+P   FPG K
Sbjct: 211 FERVLEGAFSPPFKFPGSK 229



 Score = 70.1 bits (170), Expect(2) = 2e-29
 Identities = 44/86 (51%), Positives = 50/86 (58%)
 Frame = -2

Query: 258 SQATRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXX 79
           S+ +RAK+AR +IEKILVE +R KR                +LSRLV  LIRG       
Sbjct: 228 SKFSRAKNARLKIEKILVEVIREKRREIELGEEKEGKLDEALLSRLVKALIRGEVSEEEV 287

Query: 78  XXXXXXXVFAAHDTTSFAIAMTFRML 1
                  VFAAHDTTSFAIAMTFRML
Sbjct: 288 VDNVVLLVFAAHDTTSFAIAMTFRML 313



 Score =  124 bits (310), Expect = 6e-28
 Identities = 79/169 (46%), Positives = 103/169 (60%), Gaps = 18/169 (10%)
 Frame = -3

Query: 887 LCSLVFLFGLLCLYSNV------HFDHHVLDTQKKE*PQKPPGEMGLPWIGETIDFNKSQ 726
           L +  F F LL  +  V      H+  +   T+KK     PPGEMGLPWIGETI+F K+Q
Sbjct: 8   LINFPFSFSLLIFFIIVFSILMRHYKGYKHITKKK----LPPGEMGLPWIGETIEFYKAQ 63

Query: 725 QK-RLFEEFVQPRITKHGKIFKTSLTGSPT---------ESFL--HVK*VQAGNKLVALF 582
           +K +LFEEFVQPRI K+GK FKT L GSPT           FL    K V +     ++ 
Sbjct: 64  KKNKLFEEFVQPRIEKYGKTFKTRLMGSPTVVVCGAEANMFFLSNEFKLVISSWPTSSVE 123

Query: 581 VGAVHGNMEKQGEVHRLLRGVIATSLGPAGLEAIVPKMCNSIQMHLEKN 435
           +   +  MEKQG+ HR LRG+I++SL    L+A+VPK+CN+IQ HL+KN
Sbjct: 124 LMGKNCIMEKQGDAHRCLRGIISSSLASFSLDAMVPKICNTIQSHLDKN 172


>ref|XP_009758604.1| PREDICTED: taxadiene 5-alpha hydroxylase-like [Nicotiana
           sylvestris]
          Length = 485

 Score = 89.4 bits (220), Expect(2) = 2e-29
 Identities = 58/139 (41%), Positives = 69/139 (49%), Gaps = 6/139 (4%)
 Frame = -1

Query: 643 QQNHFFMSNEFKLVISSWPSSSVQFMGT------WKNKARCTGSFVXXXXXXXXXXXXXX 482
           + N FF+SNEFKLVISSWP+SSV+ MG         +  RC    +              
Sbjct: 100 EANMFFLSNEFKLVISSWPTSSVELMGKNCIMEKQGDAHRCLRGIISSSLASFSLDAMVP 159

Query: 481 XSQKCATQFKCT*KRIGTQLRNN*HLSETISIYGNAKLLTFTIVFECLLGIGVEPGMLEI 302
               C T      K    Q        + I +Y   K LTF IVFEC LGI V+PG+LE 
Sbjct: 160 KI--CNTIQSHLDKNCNGQ-------DQIIKLYHLTKSLTFKIVFECFLGIVVKPGLLET 210

Query: 301 FERVLEGVFTPAISFPGYK 245
           FERVLEG F+P   FPG K
Sbjct: 211 FERVLEGAFSPPFKFPGSK 229



 Score = 70.1 bits (170), Expect(2) = 2e-29
 Identities = 44/86 (51%), Positives = 50/86 (58%)
 Frame = -2

Query: 258 SQATRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXX 79
           S+ +RAK+AR +IEKILVE +R KR                +LSRLV  LIRG       
Sbjct: 228 SKFSRAKNARLKIEKILVEVIREKRREIELGEEKEGKLDEALLSRLVKALIRGEVSEEEV 287

Query: 78  XXXXXXXVFAAHDTTSFAIAMTFRML 1
                  VFAAHDTTSFAIAMTFRML
Sbjct: 288 VDNVVLLVFAAHDTTSFAIAMTFRML 313



 Score =  124 bits (310), Expect = 6e-28
 Identities = 79/169 (46%), Positives = 103/169 (60%), Gaps = 18/169 (10%)
 Frame = -3

Query: 887 LCSLVFLFGLLCLYSNV------HFDHHVLDTQKKE*PQKPPGEMGLPWIGETIDFNKSQ 726
           L +  F F LL  +  V      H+  +   T+KK     PPGEMGLPWIGETI+F K+Q
Sbjct: 8   LINFPFSFSLLIFFIIVFSILMRHYKGYKHITKKK----LPPGEMGLPWIGETIEFYKAQ 63

Query: 725 QK-RLFEEFVQPRITKHGKIFKTSLTGSPT---------ESFL--HVK*VQAGNKLVALF 582
           +K +LFEEFVQPRI K+GK FKT L GSPT           FL    K V +     ++ 
Sbjct: 64  KKNKLFEEFVQPRIEKYGKTFKTRLMGSPTVLVCGAEANMFFLSNEFKLVISSWPTSSVE 123

Query: 581 VGAVHGNMEKQGEVHRLLRGVIATSLGPAGLEAIVPKMCNSIQMHLEKN 435
           +   +  MEKQG+ HR LRG+I++SL    L+A+VPK+CN+IQ HL+KN
Sbjct: 124 LMGKNCIMEKQGDAHRCLRGIISSSLASFSLDAMVPKICNTIQSHLDKN 172


>ref|XP_016463037.1| PREDICTED: taxadiene 5-alpha hydroxylase-like [Nicotiana tabacum]
          Length = 485

 Score = 89.4 bits (220), Expect(2) = 2e-29
 Identities = 58/139 (41%), Positives = 69/139 (49%), Gaps = 6/139 (4%)
 Frame = -1

Query: 643 QQNHFFMSNEFKLVISSWPSSSVQFMGT------WKNKARCTGSFVXXXXXXXXXXXXXX 482
           + N FF+SNEFKLVISSWP+SSV+ MG         +  RC    +              
Sbjct: 100 EANMFFLSNEFKLVISSWPTSSVELMGKNCIMEKQGDAHRCLRGIISSSLASFSLDAMVP 159

Query: 481 XSQKCATQFKCT*KRIGTQLRNN*HLSETISIYGNAKLLTFTIVFECLLGIGVEPGMLEI 302
               C T      K    Q        + I +Y   K LTF IVFEC LGI V+PG+LE 
Sbjct: 160 KI--CNTIQSHLDKNCNGQ-------DQIIKLYHLTKSLTFKIVFECFLGIVVKPGLLET 210

Query: 301 FERVLEGVFTPAISFPGYK 245
           FERVLEG F+P   FPG K
Sbjct: 211 FERVLEGAFSPPFKFPGSK 229



 Score = 70.1 bits (170), Expect(2) = 2e-29
 Identities = 44/86 (51%), Positives = 50/86 (58%)
 Frame = -2

Query: 258 SQATRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXX 79
           S+ +RAK+AR +IEKILVE +R KR                +LSRLV  LIRG       
Sbjct: 228 SKFSRAKNARLKIEKILVEVIREKRREIELGEEKEGKLDEALLSRLVKALIRGEVSEEEV 287

Query: 78  XXXXXXXVFAAHDTTSFAIAMTFRML 1
                  VFAAHDTTSFAIAMTFRML
Sbjct: 288 VDNVVLLVFAAHDTTSFAIAMTFRML 313



 Score =  122 bits (306), Expect = 2e-27
 Identities = 73/146 (50%), Positives = 95/146 (65%), Gaps = 12/146 (8%)
 Frame = -3

Query: 836 HFDHHVLDTQKKE*PQKPPGEMGLPWIGETIDFNKSQQK-RLFEEFVQPRITKHGKIFKT 660
           H+  +   T+KK     PPGEMGLPWIGETI+F K+Q+K +LFEEFVQPRI K+GK FKT
Sbjct: 31  HYKGYKHITKKK----LPPGEMGLPWIGETIEFYKAQKKNKLFEEFVQPRIEKYGKTFKT 86

Query: 659 SLTGSPT---------ESFL--HVK*VQAGNKLVALFVGAVHGNMEKQGEVHRLLRGVIA 513
            L GSPT           FL    K V +     ++ +   +  MEKQG+ HR LRG+I+
Sbjct: 87  RLMGSPTVVVCGAEANMFFLSNEFKLVISSWPTSSVELMGKNCIMEKQGDAHRCLRGIIS 146

Query: 512 TSLGPAGLEAIVPKMCNSIQMHLEKN 435
           +SL    L+A+VPK+CN+IQ HL+KN
Sbjct: 147 SSLASFSLDAMVPKICNTIQSHLDKN 172


>ref|XP_007141387.1| hypothetical protein PHAVU_008G191000g [Phaseolus vulgaris]
 gb|ESW13381.1| hypothetical protein PHAVU_008G191000g [Phaseolus vulgaris]
          Length = 478

 Score =  128 bits (321), Expect = 2e-29
 Identities = 76/158 (48%), Positives = 97/158 (61%), Gaps = 12/158 (7%)
 Frame = -3

Query: 869 LFGLLCLYSNVHFDHHVLDTQKKE*PQKPPGEMGLPWIGETIDF-NKSQQKRLFEEFVQP 693
           LF LL L  + HF  H  + + K+  + PPGEMGLP IGET++F N  ++ +L+EEF  P
Sbjct: 13  LFPLLLLLVSFHFLRH--EHRCKDKRKLPPGEMGLPLIGETMEFFNAQRRNKLYEEFFHP 70

Query: 692 RITKHGKIFKTSLTGSPT--------ESFL---HVK*VQAGNKLVALFVGAVHGNMEKQG 546
           RI KHGKIF+T + GSPT          FL     K V++     ++ +      MEK G
Sbjct: 71  RILKHGKIFRTRIMGSPTVVVNGAEANKFLLSNEFKLVKSSWPSSSVELMGRDSIMEKDG 130

Query: 545 EVHRLLRGVIATSLGPAGLEAIVPKMCNSIQMHLEKNW 432
           E HR LRGVI TSLG AGLE +VPK+CNS+Q HL  NW
Sbjct: 131 ERHRFLRGVIGTSLGYAGLELLVPKLCNSVQFHLATNW 168



 Score = 92.4 bits (228), Expect(2) = 2e-28
 Identities = 60/138 (43%), Positives = 76/138 (55%), Gaps = 5/138 (3%)
 Frame = -1

Query: 643 QQNHFFMSNEFKLVISSWPSSSVQFMGT----WKNKARCTGSFVXXXXXXXXXXXXXXXS 476
           + N F +SNEFKLV SSWPSSSV+ MG      K+  R                      
Sbjct: 95  EANKFLLSNEFKLVKSSWPSSSVELMGRDSIMEKDGERHRFLRGVIGTSLGYAGLELLVP 154

Query: 475 QKC-ATQFKCT*KRIGTQLRNN*HLSETISIYGNAKLLTFTIVFECLLGIGVEPGMLEIF 299
           + C + QF          L  N    + + I+ + K+L+F+IVFECLLGI VEPGML+ F
Sbjct: 155 KLCNSVQF---------HLATNWKGKKNLRIHRSTKVLSFSIVFECLLGIKVEPGMLDTF 205

Query: 298 ERVLEGVFTPAISFPGYK 245
           ERVLEGVF+PA+ FPG K
Sbjct: 206 ERVLEGVFSPAVMFPGSK 223



 Score = 63.2 bits (152), Expect(2) = 2e-28
 Identities = 40/86 (46%), Positives = 50/86 (58%)
 Frame = -2

Query: 258 SQATRAKSARKEIEKILVESVRRKRDXXXXXXXXXXXXXXXVLSRLVAGLIRGXXXXXXX 79
           S+  RAK AR EIEK+LV+ VR KR+                LS+LV+G+I+G       
Sbjct: 222 SKFWRAKKARVEIEKMLVKVVREKREEMERSLEREQDGML--LSKLVSGMIQGEISEKEV 279

Query: 78  XXXXXXXVFAAHDTTSFAIAMTFRML 1
                  VFAAHDTTSFA+AMTF+ML
Sbjct: 280 IDNVVLLVFAAHDTTSFAVAMTFKML 305