BLASTX nr result

ID: Ziziphus21_contig00018288 seq

BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ziziphus21_contig00018288
         (798 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_007010038.1| Limit dextrinase [Theobroma cacao] gi|508726...    92   3e-28
ref|XP_007220271.1| hypothetical protein PRUPE_ppa000905mg [Prun...    92   5e-28
ref|XP_010267258.1| PREDICTED: pullulanase 1, chloroplastic isof...    80   6e-28
ref|XP_010267259.1| PREDICTED: pullulanase 1, chloroplastic isof...    80   6e-28
gb|KHG14018.1| Pullulanase 1, chloroplastic -like protein [Gossy...    87   8e-28
ref|XP_002271820.3| PREDICTED: pullulanase 1, chloroplastic isof...    89   8e-28
ref|XP_010658333.1| PREDICTED: pullulanase 1, chloroplastic isof...    89   8e-28
ref|XP_008233250.1| PREDICTED: pullulanase 1, chloroplastic [Pru...    92   1e-27
ref|XP_009368969.1| PREDICTED: pullulanase 1, chloroplastic-like...    90   1e-27
ref|XP_009368970.1| PREDICTED: pullulanase 1, chloroplastic-like...    90   1e-27
ref|XP_011024340.1| PREDICTED: pullulanase 1, chloroplastic isof...    92   1e-27
ref|XP_011024341.1| PREDICTED: pullulanase 1, chloroplastic isof...    92   1e-27
ref|XP_006378784.1| hypothetical protein POPTR_0010s23600g [Popu...    92   1e-27
ref|XP_002315334.2| pullulanase family protein [Populus trichoca...    92   1e-27
ref|XP_009587793.1| PREDICTED: pullulanase 1, chloroplastic isof...    79   1e-27
ref|XP_009791833.1| PREDICTED: pullulanase 1, chloroplastic isof...    80   2e-27
ref|XP_009587786.1| PREDICTED: pullulanase 1, chloroplastic isof...    79   2e-27
ref|XP_009791834.1| PREDICTED: pullulanase 1, chloroplastic isof...    80   2e-27
ref|XP_009587790.1| PREDICTED: pullulanase 1, chloroplastic isof...    79   2e-27
ref|XP_009587791.1| PREDICTED: pullulanase 1, chloroplastic isof...    79   2e-27

>ref|XP_007010038.1| Limit dextrinase [Theobroma cacao] gi|508726951|gb|EOY18848.1|
           Limit dextrinase [Theobroma cacao]
          Length = 965

 Score = 79.3 bits (194), Expect(3) = 3e-28
 Identities = 33/42 (78%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K+LD DSYN GDWFNRLDFTY+SN WGVG PP+EKN  NWPL
Sbjct: 766 KSLDRDSYNSGDWFNRLDFTYHSNNWGVGLPPKEKNEKNWPL 807



 Score = 68.9 bits (167), Expect(3) = 3e-28
 Identities = 33/46 (71%), Positives = 38/46 (82%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           +QP LAD  FKP+ +HILAAV NF N+LRI YSSPLF L+TANAIQ
Sbjct: 808 IQPRLADPSFKPQRSHILAAVENFMNVLRIRYSSPLFRLRTANAIQ 853



 Score = 25.4 bits (54), Expect(3) = 3e-28
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L+ G   FH+GD MLRSK L
Sbjct: 749 LSQGIPFFHAGDEMLRSKSL 768



 Score = 92.0 bits (227), Expect(2) = 1e-23
 Identities = 51/105 (48%), Positives = 59/105 (56%), Gaps = 30/105 (28%)
 Frame = -2

Query: 665 EDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPGS 576
           E NGVW TKGPK                               R LSSD +RTLFVN  S
Sbjct: 244 ETNGVWSTKGPKRWEGCYYVYEVSVYHPSTLQIEKCYANDPYARGLSSDGRRTLFVNLDS 303

Query: 575 DTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441
           + LKPEGWD LAD+KPD+LS  DISIYELH+ DFSA+D+ +   F
Sbjct: 304 NGLKPEGWDELADKKPDILSFSDISIYELHIRDFSANDNTVNPDF 348



 Score = 45.8 bits (107), Expect(2) = 1e-23
 Identities = 24/41 (58%), Positives = 28/41 (68%)
 Frame = -1

Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV 676
           PLGA Y  + VSL+LWAPTA Q V A IYKD    +P EI+
Sbjct: 201 PLGAHYLGEVVSLYLWAPTA-QAVHAHIYKDPMGGSPLEII 240


>ref|XP_007220271.1| hypothetical protein PRUPE_ppa000905mg [Prunus persica]
           gi|462416733|gb|EMJ21470.1| hypothetical protein
           PRUPE_ppa000905mg [Prunus persica]
          Length = 965

 Score = 77.4 bits (189), Expect(3) = 5e-28
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K+LD DSYN GDWFNRLDFTY+SN WGVG PP+EKN  +WPL
Sbjct: 772 KSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEKSWPL 813



 Score = 69.3 bits (168), Expect(3) = 5e-28
 Identities = 33/45 (73%), Positives = 39/45 (86%)
 Frame = -2

Query: 164 QPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           +P LAD  FKP+++HI+AAV NFSNLLRI YSSPLF L+TANAIQ
Sbjct: 815 KPRLADPSFKPQKSHIIAAVENFSNLLRIRYSSPLFRLRTANAIQ 859



 Score = 26.2 bits (56), Expect(3) = 5e-28
 Identities = 13/20 (65%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           LA G   FHSGD +LRSK L
Sbjct: 755 LAQGIPFFHSGDEILRSKSL 774



 Score = 92.0 bits (227), Expect(2) = 4e-27
 Identities = 50/105 (47%), Positives = 57/105 (54%), Gaps = 30/105 (28%)
 Frame = -2

Query: 665 EDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPGS 576
           E NGVW TKGPK                               R LSSD +RTL VN  S
Sbjct: 250 EFNGVWSTKGPKSWEGCYYVYEVSVYHPSTLRIEKCYANDPYARGLSSDGRRTLLVNLDS 309

Query: 575 DTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441
           D +KPEGWD L D+KPD+LS  DISIYELH+ DFSASD  +  +F
Sbjct: 310 DNIKPEGWDKLVDEKPDILSFSDISIYELHIRDFSASDQAVHPEF 354



 Score = 57.8 bits (138), Expect(2) = 4e-27
 Identities = 29/44 (65%), Positives = 35/44 (79%)
 Frame = -1

Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667
           PLGALYS ++VSL+LWAPTA QEV  CIYK+ S  +P E+V LE
Sbjct: 207 PLGALYSKQSVSLYLWAPTA-QEVCVCIYKEPSGGSPLEVVQLE 249


>ref|XP_010267258.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Nelumbo
           nucifera]
          Length = 958

 Score = 78.6 bits (192), Expect(3) = 6e-28
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K+LD DSYN GDWFNRLDF+YN+N WGVG PP+EKN  NWPL
Sbjct: 765 KSLDRDSYNSGDWFNRLDFSYNTNNWGVGLPPKEKNEKNWPL 806



 Score = 68.6 bits (166), Expect(3) = 6e-28
 Identities = 32/46 (69%), Positives = 39/46 (84%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           ++P LAD  FKP++ HILAAV NFSN+LRI YSSPLF L+TAN+IQ
Sbjct: 807 IKPRLADPSFKPQKRHILAAVENFSNMLRIRYSSPLFRLRTANSIQ 852



 Score = 25.4 bits (54), Expect(3) = 6e-28
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L+ G   FHSGD +LRSK L
Sbjct: 748 LSQGIPFFHSGDELLRSKSL 767



 Score = 80.5 bits (197), Expect(2) = 2e-20
 Identities = 39/65 (60%), Positives = 45/65 (69%)
 Frame = -2

Query: 635 PKDRRLSSDAQRTLFVNPGSDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHV 456
           P  R LSSD +RTL VN   D LKP+GWD LAD+KP + S  DISIYELH+ DFSASD  
Sbjct: 284 PYARGLSSDGKRTLLVNLDCDALKPDGWDALADEKPKLSSFSDISIYELHIRDFSASDQT 343

Query: 455 METKF 441
           +   F
Sbjct: 344 VHPDF 348



 Score = 46.6 bits (109), Expect(2) = 2e-20
 Identities = 26/44 (59%), Positives = 31/44 (70%)
 Frame = -1

Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667
           PLGA+++ +AVSL+LWAPTA Q V A IY D S   P E V LE
Sbjct: 201 PLGAVFTKEAVSLYLWAPTA-QAVSAIIYGDSSGGVPLESVQLE 243


>ref|XP_010267259.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Nelumbo
           nucifera]
          Length = 941

 Score = 78.6 bits (192), Expect(3) = 6e-28
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K+LD DSYN GDWFNRLDF+YN+N WGVG PP+EKN  NWPL
Sbjct: 748 KSLDRDSYNSGDWFNRLDFSYNTNNWGVGLPPKEKNEKNWPL 789



 Score = 68.6 bits (166), Expect(3) = 6e-28
 Identities = 32/46 (69%), Positives = 39/46 (84%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           ++P LAD  FKP++ HILAAV NFSN+LRI YSSPLF L+TAN+IQ
Sbjct: 790 IKPRLADPSFKPQKRHILAAVENFSNMLRIRYSSPLFRLRTANSIQ 835



 Score = 25.4 bits (54), Expect(3) = 6e-28
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L+ G   FHSGD +LRSK L
Sbjct: 731 LSQGIPFFHSGDELLRSKSL 750



 Score = 80.5 bits (197), Expect(2) = 2e-20
 Identities = 39/65 (60%), Positives = 45/65 (69%)
 Frame = -2

Query: 635 PKDRRLSSDAQRTLFVNPGSDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHV 456
           P  R LSSD +RTL VN   D LKP+GWD LAD+KP + S  DISIYELH+ DFSASD  
Sbjct: 284 PYARGLSSDGKRTLLVNLDCDALKPDGWDALADEKPKLSSFSDISIYELHIRDFSASDQT 343

Query: 455 METKF 441
           +   F
Sbjct: 344 VHPDF 348



 Score = 46.6 bits (109), Expect(2) = 2e-20
 Identities = 26/44 (59%), Positives = 31/44 (70%)
 Frame = -1

Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667
           PLGA+++ +AVSL+LWAPTA Q V A IY D S   P E V LE
Sbjct: 201 PLGAVFTKEAVSLYLWAPTA-QAVSAIIYGDSSGGVPLESVQLE 243


>gb|KHG14018.1| Pullulanase 1, chloroplastic -like protein [Gossypium arboreum]
          Length = 1016

 Score = 77.8 bits (190), Expect(3) = 8e-28
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K+LD DSYN GDWFNRLDFTYNSN WGVG PP++KN  +WPL
Sbjct: 802 KSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPKQKNENSWPL 843



 Score = 69.3 bits (168), Expect(3) = 8e-28
 Identities = 33/47 (70%), Positives = 40/47 (85%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQL 27
           +QP LAD  FKP+ +HILAAV NF+++LRI YSSPLF L+TANAIQL
Sbjct: 844 IQPRLADPSFKPQRSHILAAVENFTDVLRIRYSSPLFRLRTANAIQL 890



 Score = 25.0 bits (53), Expect(3) = 8e-28
 Identities = 12/20 (60%), Positives = 13/20 (65%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L  G   FH+GD MLRSK L
Sbjct: 785 LTQGIPFFHAGDEMLRSKSL 804



 Score = 86.7 bits (213), Expect(2) = 6e-23
 Identities = 48/105 (45%), Positives = 56/105 (53%), Gaps = 30/105 (28%)
 Frame = -2

Query: 665 EDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPGS 576
           E NGVW TKGPK                               R LS+D +RTLFVN  +
Sbjct: 242 ETNGVWSTKGPKSWEGCYYMYEISVYHPSTLHIEKCYANDPYARGLSADGKRTLFVNLHA 301

Query: 575 DTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441
           D LKPEGW+ LAD K D+LS  DISIYELH+ DFSA+D  +   F
Sbjct: 302 DDLKPEGWNELADMKSDILSFSDISIYELHIRDFSANDDTVNADF 346



 Score = 48.9 bits (115), Expect(2) = 6e-23
 Identities = 28/44 (63%), Positives = 31/44 (70%)
 Frame = -1

Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667
           PLGA YS +AVSL LWAPTA Q V A IYKD    +P EI+ LE
Sbjct: 199 PLGAFYSAEAVSLCLWAPTA-QAVYAHIYKDPVGGSPLEIIPLE 241


>ref|XP_002271820.3| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Vitis vinifera]
           gi|296085954|emb|CBI31395.3| unnamed protein product
           [Vitis vinifera]
          Length = 956

 Score = 79.0 bits (193), Expect(3) = 8e-28
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K++D DSYN GDWFNRLDF+YNSN WGVG PP+EKN  NWPL
Sbjct: 763 KSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEKNWPL 804



 Score = 67.4 bits (163), Expect(3) = 8e-28
 Identities = 31/46 (67%), Positives = 39/46 (84%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           ++P LAD  FKP++NHI+AAV NF NLL+I YSSPLF L+TAN+IQ
Sbjct: 805 IKPRLADPSFKPQKNHIIAAVENFLNLLKIRYSSPLFRLRTANSIQ 850



 Score = 25.8 bits (55), Expect(3) = 8e-28
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L+ G   FHSGD MLRSK +
Sbjct: 746 LSQGIPFFHSGDEMLRSKSI 765



 Score = 89.0 bits (219), Expect(2) = 2e-25
 Identities = 50/105 (47%), Positives = 56/105 (53%), Gaps = 30/105 (28%)
 Frame = -2

Query: 665 EDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPGS 576
           E NGVW   GPK                               R LS+DA+RTL VN  S
Sbjct: 241 EFNGVWSASGPKSWEGCYYEYEVSVYHPSTLQIEKCTANDPYARGLSADARRTLLVNLDS 300

Query: 575 DTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441
           D LKPEGWD LAD+KP +LS  DISIYELH+ DFSASDH +   F
Sbjct: 301 DDLKPEGWDNLADEKPALLSFSDISIYELHIRDFSASDHTVHPDF 345



 Score = 54.7 bits (130), Expect(2) = 2e-25
 Identities = 28/44 (63%), Positives = 34/44 (77%)
 Frame = -1

Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667
           PLGA++S +AV+L+LWAPTA Q V ACIY D S  +P EIV LE
Sbjct: 198 PLGAIFSKEAVTLYLWAPTA-QVVHACIYGDPSGGDPLEIVCLE 240


>ref|XP_010658333.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Vitis vinifera]
          Length = 954

 Score = 79.0 bits (193), Expect(3) = 8e-28
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K++D DSYN GDWFNRLDF+YNSN WGVG PP+EKN  NWPL
Sbjct: 761 KSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEKNWPL 802



 Score = 67.4 bits (163), Expect(3) = 8e-28
 Identities = 31/46 (67%), Positives = 39/46 (84%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           ++P LAD  FKP++NHI+AAV NF NLL+I YSSPLF L+TAN+IQ
Sbjct: 803 IKPRLADPSFKPQKNHIIAAVENFLNLLKIRYSSPLFRLRTANSIQ 848



 Score = 25.8 bits (55), Expect(3) = 8e-28
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L+ G   FHSGD MLRSK +
Sbjct: 744 LSQGIPFFHSGDEMLRSKSI 763



 Score = 89.0 bits (219), Expect(2) = 2e-25
 Identities = 50/105 (47%), Positives = 56/105 (53%), Gaps = 30/105 (28%)
 Frame = -2

Query: 665 EDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPGS 576
           E NGVW   GPK                               R LS+DA+RTL VN  S
Sbjct: 239 EFNGVWSASGPKSWEGCYYEYEVSVYHPSTLQIEKCTANDPYARGLSADARRTLLVNLDS 298

Query: 575 DTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441
           D LKPEGWD LAD+KP +LS  DISIYELH+ DFSASDH +   F
Sbjct: 299 DDLKPEGWDNLADEKPALLSFSDISIYELHIRDFSASDHTVHPDF 343



 Score = 54.7 bits (130), Expect(2) = 2e-25
 Identities = 28/44 (63%), Positives = 34/44 (77%)
 Frame = -1

Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667
           PLGA++S +AV+L+LWAPTA Q V ACIY D S  +P EIV LE
Sbjct: 196 PLGAIFSKEAVTLYLWAPTA-QVVHACIYGDPSGGDPLEIVCLE 238


>ref|XP_008233250.1| PREDICTED: pullulanase 1, chloroplastic [Prunus mume]
          Length = 967

 Score = 92.4 bits (228), Expect(2) = 1e-27
 Identities = 50/105 (47%), Positives = 58/105 (55%), Gaps = 30/105 (28%)
 Frame = -2

Query: 665 EDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPGS 576
           E NGVW TKGPK                               R LSSD++RTL VN  S
Sbjct: 250 EFNGVWSTKGPKSWEGCYYVYEVSVYHPSTLRIEKCYANDPYARGLSSDSRRTLLVNLDS 309

Query: 575 DTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441
           D +KPEGWD L D+KPD+LS  DISIYELH+ DFSASD  +  +F
Sbjct: 310 DNIKPEGWDKLVDEKPDILSFSDISIYELHIRDFSASDQAVHPEF 354



 Score = 59.3 bits (142), Expect(2) = 1e-27
 Identities = 30/44 (68%), Positives = 35/44 (79%)
 Frame = -1

Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667
           PLGALYS ++VSL+LWAPTA QEV  CIYKD S  +P E+V LE
Sbjct: 207 PLGALYSKQSVSLYLWAPTA-QEVCVCIYKDPSGGSPLEVVQLE 249



 Score = 77.4 bits (189), Expect(3) = 2e-27
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K+LD DSYN GDWFNRLDFTY+SN WGVG PP+EKN  +WPL
Sbjct: 774 KSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEKSWPL 815



 Score = 67.0 bits (162), Expect(3) = 2e-27
 Identities = 32/45 (71%), Positives = 37/45 (82%)
 Frame = -2

Query: 164 QPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           +P LAD  FKP+ +HI+AAV NFSNLL I YSSPLF L+TANAIQ
Sbjct: 817 KPRLADPSFKPQRSHIIAAVENFSNLLHIRYSSPLFRLRTANAIQ 861



 Score = 26.2 bits (56), Expect(3) = 2e-27
 Identities = 13/20 (65%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           LA G   FHSGD +LRSK L
Sbjct: 757 LAQGIPFFHSGDEILRSKSL 776


>ref|XP_009368969.1| PREDICTED: pullulanase 1, chloroplastic-like isoform X1 [Pyrus x
           bretschneideri]
          Length = 978

 Score = 77.0 bits (188), Expect(3) = 1e-27
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K+LD DSYN GDWFNRLDFTY+SN WGVG PP+EKN  +WPL
Sbjct: 785 KSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEHSWPL 826



 Score = 70.9 bits (172), Expect(3) = 1e-27
 Identities = 34/46 (73%), Positives = 40/46 (86%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           ++P LAD  FKP+++HILAAV NFSNLLRI YSSPLF L+TANAIQ
Sbjct: 827 MKPRLADPSFKPQKSHILAAVENFSNLLRIRYSSPLFRLRTANAIQ 872



 Score = 23.9 bits (50), Expect(3) = 1e-27
 Identities = 11/20 (55%), Positives = 13/20 (65%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L  G   FH+GD +LRSK L
Sbjct: 768 LGQGIPFFHAGDELLRSKSL 787



 Score = 90.1 bits (222), Expect(2) = 2e-26
 Identities = 49/105 (46%), Positives = 58/105 (55%), Gaps = 30/105 (28%)
 Frame = -2

Query: 665 EDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPGS 576
           E NGVW TKGPK                               R LSSD +RTL VN  S
Sbjct: 263 EVNGVWSTKGPKSWEGCYYVYEVSVYHPSTLKIEKCYANDPYARGLSSDGRRTLLVNLDS 322

Query: 575 DTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441
           D +KPEGWD LAD+KPD+ S  DISIYELH+ DFSA+D  + ++F
Sbjct: 323 DDIKPEGWDKLADEKPDINSFSDISIYELHIRDFSANDQTVHSEF 367



 Score = 57.4 bits (137), Expect(2) = 2e-26
 Identities = 30/45 (66%), Positives = 36/45 (80%), Gaps = 1/45 (2%)
 Frame = -1

Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKD-VSADNPQEIV*LE 667
           PLGALYS ++VSL+LWAPTA QEV  CIYK+ +   NPQE+V LE
Sbjct: 219 PLGALYSEESVSLYLWAPTA-QEVCVCIYKEPLGGGNPQEVVQLE 262


>ref|XP_009368970.1| PREDICTED: pullulanase 1, chloroplastic-like isoform X2 [Pyrus x
           bretschneideri]
          Length = 977

 Score = 77.0 bits (188), Expect(3) = 1e-27
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K+LD DSYN GDWFNRLDFTY+SN WGVG PP+EKN  +WPL
Sbjct: 784 KSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEHSWPL 825



 Score = 70.9 bits (172), Expect(3) = 1e-27
 Identities = 34/46 (73%), Positives = 40/46 (86%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           ++P LAD  FKP+++HILAAV NFSNLLRI YSSPLF L+TANAIQ
Sbjct: 826 MKPRLADPSFKPQKSHILAAVENFSNLLRIRYSSPLFRLRTANAIQ 871



 Score = 23.9 bits (50), Expect(3) = 1e-27
 Identities = 11/20 (55%), Positives = 13/20 (65%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L  G   FH+GD +LRSK L
Sbjct: 767 LGQGIPFFHAGDELLRSKSL 786



 Score = 90.1 bits (222), Expect(2) = 2e-26
 Identities = 49/105 (46%), Positives = 58/105 (55%), Gaps = 30/105 (28%)
 Frame = -2

Query: 665 EDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPGS 576
           E NGVW TKGPK                               R LSSD +RTL VN  S
Sbjct: 262 EVNGVWSTKGPKSWEGCYYVYEVSVYHPSTLKIEKCYANDPYARGLSSDGRRTLLVNLDS 321

Query: 575 DTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441
           D +KPEGWD LAD+KPD+ S  DISIYELH+ DFSA+D  + ++F
Sbjct: 322 DDIKPEGWDKLADEKPDINSFSDISIYELHIRDFSANDQTVHSEF 366



 Score = 57.4 bits (137), Expect(2) = 2e-26
 Identities = 30/45 (66%), Positives = 36/45 (80%), Gaps = 1/45 (2%)
 Frame = -1

Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKD-VSADNPQEIV*LE 667
           PLGALYS ++VSL+LWAPTA QEV  CIYK+ +   NPQE+V LE
Sbjct: 218 PLGALYSEESVSLYLWAPTA-QEVCVCIYKEPLGGGNPQEVVQLE 261


>ref|XP_011024340.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Populus
           euphratica]
          Length = 971

 Score = 78.2 bits (191), Expect(3) = 1e-27
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K+LD DSYN GDWFNRLDFTYNSN WGVG PP++KN  +WPL
Sbjct: 780 KSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPKQKNEKHWPL 821



 Score = 66.6 bits (161), Expect(3) = 1e-27
 Identities = 31/46 (67%), Positives = 39/46 (84%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           ++P LAD  FKP++NHILAA+NNF ++L+I YSSPLF L TANAIQ
Sbjct: 822 IRPRLADPSFKPQKNHILAAINNFLDVLQIRYSSPLFRLTTANAIQ 867



 Score = 26.6 bits (57), Expect(3) = 1e-27
 Identities = 13/20 (65%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L+ G   FHSGD MLRSK L
Sbjct: 763 LSQGIPFFHSGDEMLRSKSL 782



 Score = 91.7 bits (226), Expect = 6e-16
 Identities = 51/106 (48%), Positives = 59/106 (55%), Gaps = 30/106 (28%)
 Frame = -2

Query: 668 REDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPG 579
           +E NGVW  +G KD                              R LS D+QRTLFVN  
Sbjct: 257 KEVNGVWSVEGSKDWEGCYYVYEVNVYHPSTLRVEKCYANDPYARGLSPDSQRTLFVNLD 316

Query: 578 SDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441
           SDTLKPEGW+ LAD+KP +LS  DISIYELHV DFSA+DH +   F
Sbjct: 317 SDTLKPEGWEKLADEKPIILSFSDISIYELHVRDFSANDHTVHPDF 362


>ref|XP_011024341.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Populus
           euphratica]
          Length = 969

 Score = 78.2 bits (191), Expect(3) = 1e-27
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K+LD DSYN GDWFNRLDFTYNSN WGVG PP++KN  +WPL
Sbjct: 778 KSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPKQKNEKHWPL 819



 Score = 66.6 bits (161), Expect(3) = 1e-27
 Identities = 31/46 (67%), Positives = 39/46 (84%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           ++P LAD  FKP++NHILAA+NNF ++L+I YSSPLF L TANAIQ
Sbjct: 820 IRPRLADPSFKPQKNHILAAINNFLDVLQIRYSSPLFRLTTANAIQ 865



 Score = 26.6 bits (57), Expect(3) = 1e-27
 Identities = 13/20 (65%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L+ G   FHSGD MLRSK L
Sbjct: 761 LSQGIPFFHSGDEMLRSKSL 780



 Score = 91.7 bits (226), Expect = 6e-16
 Identities = 51/106 (48%), Positives = 59/106 (55%), Gaps = 30/106 (28%)
 Frame = -2

Query: 668 REDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPG 579
           +E NGVW  +G KD                              R LS D+QRTLFVN  
Sbjct: 255 KEVNGVWSVEGSKDWEGCYYVYEVNVYHPSTLRVEKCYANDPYARGLSPDSQRTLFVNLD 314

Query: 578 SDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441
           SDTLKPEGW+ LAD+KP +LS  DISIYELHV DFSA+DH +   F
Sbjct: 315 SDTLKPEGWEKLADEKPIILSFSDISIYELHVRDFSANDHTVHPDF 360


>ref|XP_006378784.1| hypothetical protein POPTR_0010s23600g [Populus trichocarpa]
           gi|550330451|gb|ERP56581.1| hypothetical protein
           POPTR_0010s23600g [Populus trichocarpa]
          Length = 945

 Score = 78.2 bits (191), Expect(3) = 1e-27
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K+LD DSYN GDWFNRLDFTYNSN WGVG PP++KN  +WPL
Sbjct: 780 KSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPKQKNEKHWPL 821



 Score = 66.6 bits (161), Expect(3) = 1e-27
 Identities = 31/46 (67%), Positives = 39/46 (84%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           ++P LAD  FKP++NHILAA+NNF ++L+I YSSPLF L TANAIQ
Sbjct: 822 IRPRLADPSFKPQKNHILAAINNFLDVLQIRYSSPLFRLTTANAIQ 867



 Score = 26.6 bits (57), Expect(3) = 1e-27
 Identities = 13/20 (65%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L+ G   FHSGD MLRSK L
Sbjct: 763 LSQGIPFFHSGDEMLRSKSL 782



 Score = 91.7 bits (226), Expect = 6e-16
 Identities = 51/106 (48%), Positives = 59/106 (55%), Gaps = 30/106 (28%)
 Frame = -2

Query: 668 REDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPG 579
           +E NGVW  +G KD                              R LS D+QRTLFVN  
Sbjct: 257 KEVNGVWSVEGSKDWEGCYYVYEVSVYHPSTLHVEKCYANDPYARGLSPDSQRTLFVNLD 316

Query: 578 SDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441
           SDTLKPEGW+ LAD+KP +LS  DISIYELHV DFSA+DH +   F
Sbjct: 317 SDTLKPEGWEKLADEKPIILSFSDISIYELHVRDFSANDHTVHPDF 362


>ref|XP_002315334.2| pullulanase family protein [Populus trichocarpa]
           gi|550330450|gb|EEF01505.2| pullulanase family protein
           [Populus trichocarpa]
          Length = 943

 Score = 78.2 bits (191), Expect(3) = 1e-27
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K+LD DSYN GDWFNRLDFTYNSN WGVG PP++KN  +WPL
Sbjct: 778 KSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPKQKNEKHWPL 819



 Score = 66.6 bits (161), Expect(3) = 1e-27
 Identities = 31/46 (67%), Positives = 39/46 (84%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           ++P LAD  FKP++NHILAA+NNF ++L+I YSSPLF L TANAIQ
Sbjct: 820 IRPRLADPSFKPQKNHILAAINNFLDVLQIRYSSPLFRLTTANAIQ 865



 Score = 26.6 bits (57), Expect(3) = 1e-27
 Identities = 13/20 (65%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L+ G   FHSGD MLRSK L
Sbjct: 761 LSQGIPFFHSGDEMLRSKSL 780



 Score = 91.7 bits (226), Expect = 6e-16
 Identities = 51/106 (48%), Positives = 59/106 (55%), Gaps = 30/106 (28%)
 Frame = -2

Query: 668 REDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPG 579
           +E NGVW  +G KD                              R LS D+QRTLFVN  
Sbjct: 255 KEVNGVWSVEGSKDWEGCYYVYEVSVYHPSTLHVEKCYANDPYARGLSPDSQRTLFVNLD 314

Query: 578 SDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441
           SDTLKPEGW+ LAD+KP +LS  DISIYELHV DFSA+DH +   F
Sbjct: 315 SDTLKPEGWEKLADEKPIILSFSDISIYELHVRDFSANDHTVHPDF 360


>ref|XP_009587793.1| PREDICTED: pullulanase 1, chloroplastic isoform X5 [Nicotiana
           tomentosiformis] gi|697158090|ref|XP_009587794.1|
           PREDICTED: pullulanase 1, chloroplastic isoform X5
           [Nicotiana tomentosiformis]
          Length = 849

 Score = 79.0 bits (193), Expect(3) = 1e-27
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K++D DSYN GDWFNRLDF+YNSN WGVG PP+EKN  NWPL
Sbjct: 761 KSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPL 802



 Score = 66.6 bits (161), Expect(3) = 1e-27
 Identities = 30/47 (63%), Positives = 41/47 (87%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQL 27
           ++P LAD  +KP+++HILAAV NFSNL++I YSSPLF L+TAN+IQ+
Sbjct: 803 IKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQV 849



 Score = 25.8 bits (55), Expect(3) = 1e-27
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L+ G   FHSGD MLRSK +
Sbjct: 744 LSQGIPFFHSGDEMLRSKSI 763



 Score = 79.0 bits (193), Expect = 4e-12
 Identities = 44/106 (41%), Positives = 56/106 (52%), Gaps = 30/106 (28%)
 Frame = -2

Query: 668 REDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPG 579
           +E NGVW  KGP+                               R LS+D +R L VN  
Sbjct: 238 KELNGVWSVKGPRHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRALLVNLD 297

Query: 578 SDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441
           SD +KPEGWD L D+KP++LS  DIS+YELHV DFSA+D  +  +F
Sbjct: 298 SDDVKPEGWDNLQDEKPNLLSFSDISLYELHVRDFSANDPTVPHEF 343


>ref|XP_009791833.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Nicotiana
           sylvestris]
          Length = 952

 Score = 79.0 bits (193), Expect(3) = 2e-27
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K++D DSYN GDWFNRLDF+YNSN WGVG PP+EKN  NWPL
Sbjct: 761 KSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPL 802



 Score = 66.2 bits (160), Expect(3) = 2e-27
 Identities = 30/46 (65%), Positives = 40/46 (86%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           ++P LAD  +KP+++HILAAV NFSNL++I YSSPLF L+TAN+IQ
Sbjct: 803 IKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQ 848



 Score = 25.8 bits (55), Expect(3) = 2e-27
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L+ G   FHSGD MLRSK +
Sbjct: 744 LSQGIPFFHSGDEMLRSKSI 763



 Score = 80.1 bits (196), Expect(2) = 5e-21
 Identities = 39/65 (60%), Positives = 48/65 (73%)
 Frame = -2

Query: 635 PKDRRLSSDAQRTLFVNPGSDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHV 456
           P  R LS+D +RTL VN  SD +KPEGWD L D+KPD+LS  DIS+YELHV DFSA+D  
Sbjct: 279 PYARGLSADGKRTLLVNLVSDDVKPEGWDNLQDEKPDLLSFSDISLYELHVRDFSANDPT 338

Query: 455 METKF 441
           +  +F
Sbjct: 339 VPHEF 343



 Score = 48.9 bits (115), Expect(2) = 5e-21
 Identities = 27/44 (61%), Positives = 32/44 (72%)
 Frame = -1

Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667
           PLGA+   +AVSL+LWAPTA Q VRA IYK  S  +P EIV L+
Sbjct: 196 PLGAVCDTEAVSLYLWAPTA-QAVRALIYKSPSETDPVEIVQLK 238


>ref|XP_009587786.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Nicotiana
           tomentosiformis] gi|697158080|ref|XP_009587789.1|
           PREDICTED: pullulanase 1, chloroplastic isoform X1
           [Nicotiana tomentosiformis]
          Length = 952

 Score = 79.0 bits (193), Expect(3) = 2e-27
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K++D DSYN GDWFNRLDF+YNSN WGVG PP+EKN  NWPL
Sbjct: 761 KSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPL 802



 Score = 66.2 bits (160), Expect(3) = 2e-27
 Identities = 30/46 (65%), Positives = 40/46 (86%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           ++P LAD  +KP+++HILAAV NFSNL++I YSSPLF L+TAN+IQ
Sbjct: 803 IKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQ 848



 Score = 25.8 bits (55), Expect(3) = 2e-27
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L+ G   FHSGD MLRSK +
Sbjct: 744 LSQGIPFFHSGDEMLRSKSI 763



 Score = 79.0 bits (193), Expect = 4e-12
 Identities = 44/106 (41%), Positives = 56/106 (52%), Gaps = 30/106 (28%)
 Frame = -2

Query: 668 REDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPG 579
           +E NGVW  KGP+                               R LS+D +R L VN  
Sbjct: 238 KELNGVWSVKGPRHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRALLVNLD 297

Query: 578 SDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441
           SD +KPEGWD L D+KP++LS  DIS+YELHV DFSA+D  +  +F
Sbjct: 298 SDDVKPEGWDNLQDEKPNLLSFSDISLYELHVRDFSANDPTVPHEF 343


>ref|XP_009791834.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Nicotiana
           sylvestris]
          Length = 927

 Score = 79.0 bits (193), Expect(3) = 2e-27
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K++D DSYN GDWFNRLDF+YNSN WGVG PP+EKN  NWPL
Sbjct: 761 KSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPL 802



 Score = 66.2 bits (160), Expect(3) = 2e-27
 Identities = 30/46 (65%), Positives = 40/46 (86%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           ++P LAD  +KP+++HILAAV NFSNL++I YSSPLF L+TAN+IQ
Sbjct: 803 IKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQ 848



 Score = 25.8 bits (55), Expect(3) = 2e-27
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L+ G   FHSGD MLRSK +
Sbjct: 744 LSQGIPFFHSGDEMLRSKSI 763



 Score = 80.1 bits (196), Expect(2) = 5e-21
 Identities = 39/65 (60%), Positives = 48/65 (73%)
 Frame = -2

Query: 635 PKDRRLSSDAQRTLFVNPGSDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHV 456
           P  R LS+D +RTL VN  SD +KPEGWD L D+KPD+LS  DIS+YELHV DFSA+D  
Sbjct: 279 PYARGLSADGKRTLLVNLVSDDVKPEGWDNLQDEKPDLLSFSDISLYELHVRDFSANDPT 338

Query: 455 METKF 441
           +  +F
Sbjct: 339 VPHEF 343



 Score = 48.9 bits (115), Expect(2) = 5e-21
 Identities = 27/44 (61%), Positives = 32/44 (72%)
 Frame = -1

Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667
           PLGA+   +AVSL+LWAPTA Q VRA IYK  S  +P EIV L+
Sbjct: 196 PLGAVCDTEAVSLYLWAPTA-QAVRALIYKSPSETDPVEIVQLK 238


>ref|XP_009587790.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Nicotiana
           tomentosiformis]
          Length = 927

 Score = 79.0 bits (193), Expect(3) = 2e-27
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K++D DSYN GDWFNRLDF+YNSN WGVG PP+EKN  NWPL
Sbjct: 761 KSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPL 802



 Score = 66.2 bits (160), Expect(3) = 2e-27
 Identities = 30/46 (65%), Positives = 40/46 (86%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           ++P LAD  +KP+++HILAAV NFSNL++I YSSPLF L+TAN+IQ
Sbjct: 803 IKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQ 848



 Score = 25.8 bits (55), Expect(3) = 2e-27
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L+ G   FHSGD MLRSK +
Sbjct: 744 LSQGIPFFHSGDEMLRSKSI 763



 Score = 79.0 bits (193), Expect = 4e-12
 Identities = 44/106 (41%), Positives = 56/106 (52%), Gaps = 30/106 (28%)
 Frame = -2

Query: 668 REDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPG 579
           +E NGVW  KGP+                               R LS+D +R L VN  
Sbjct: 238 KELNGVWSVKGPRHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRALLVNLD 297

Query: 578 SDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441
           SD +KPEGWD L D+KP++LS  DIS+YELHV DFSA+D  +  +F
Sbjct: 298 SDDVKPEGWDNLQDEKPNLLSFSDISLYELHVRDFSANDPTVPHEF 343


>ref|XP_009587791.1| PREDICTED: pullulanase 1, chloroplastic isoform X3 [Nicotiana
           tomentosiformis]
          Length = 920

 Score = 79.0 bits (193), Expect(3) = 2e-27
 Identities = 32/42 (76%), Positives = 36/42 (85%)
 Frame = -3

Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182
           K++D DSYN GDWFNRLDF+YNSN WGVG PP+EKN  NWPL
Sbjct: 729 KSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPL 770



 Score = 66.2 bits (160), Expect(3) = 2e-27
 Identities = 30/46 (65%), Positives = 40/46 (86%)
 Frame = -2

Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30
           ++P LAD  +KP+++HILAAV NFSNL++I YSSPLF L+TAN+IQ
Sbjct: 771 IKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQ 816



 Score = 25.8 bits (55), Expect(3) = 2e-27
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = -1

Query: 357 LAPGNIVFHSGDGMLRSKHL 298
           L+ G   FHSGD MLRSK +
Sbjct: 712 LSQGIPFFHSGDEMLRSKSI 731



 Score = 79.0 bits (193), Expect = 4e-12
 Identities = 44/106 (41%), Positives = 56/106 (52%), Gaps = 30/106 (28%)
 Frame = -2

Query: 668 REDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPG 579
           +E NGVW  KGP+                               R LS+D +R L VN  
Sbjct: 238 KELNGVWSVKGPRHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRALLVNLD 297

Query: 578 SDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441
           SD +KPEGWD L D+KP++LS  DIS+YELHV DFSA+D  +  +F
Sbjct: 298 SDDVKPEGWDNLQDEKPNLLSFSDISLYELHVRDFSANDPTVPHEF 343


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