BLASTX nr result
ID: Ziziphus21_contig00018288
seq
BLASTX 2.2.25 [Feb-01-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Ziziphus21_contig00018288 (798 letters) Database: ./nr 77,306,371 sequences; 28,104,191,420 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|XP_007010038.1| Limit dextrinase [Theobroma cacao] gi|508726... 92 3e-28 ref|XP_007220271.1| hypothetical protein PRUPE_ppa000905mg [Prun... 92 5e-28 ref|XP_010267258.1| PREDICTED: pullulanase 1, chloroplastic isof... 80 6e-28 ref|XP_010267259.1| PREDICTED: pullulanase 1, chloroplastic isof... 80 6e-28 gb|KHG14018.1| Pullulanase 1, chloroplastic -like protein [Gossy... 87 8e-28 ref|XP_002271820.3| PREDICTED: pullulanase 1, chloroplastic isof... 89 8e-28 ref|XP_010658333.1| PREDICTED: pullulanase 1, chloroplastic isof... 89 8e-28 ref|XP_008233250.1| PREDICTED: pullulanase 1, chloroplastic [Pru... 92 1e-27 ref|XP_009368969.1| PREDICTED: pullulanase 1, chloroplastic-like... 90 1e-27 ref|XP_009368970.1| PREDICTED: pullulanase 1, chloroplastic-like... 90 1e-27 ref|XP_011024340.1| PREDICTED: pullulanase 1, chloroplastic isof... 92 1e-27 ref|XP_011024341.1| PREDICTED: pullulanase 1, chloroplastic isof... 92 1e-27 ref|XP_006378784.1| hypothetical protein POPTR_0010s23600g [Popu... 92 1e-27 ref|XP_002315334.2| pullulanase family protein [Populus trichoca... 92 1e-27 ref|XP_009587793.1| PREDICTED: pullulanase 1, chloroplastic isof... 79 1e-27 ref|XP_009791833.1| PREDICTED: pullulanase 1, chloroplastic isof... 80 2e-27 ref|XP_009587786.1| PREDICTED: pullulanase 1, chloroplastic isof... 79 2e-27 ref|XP_009791834.1| PREDICTED: pullulanase 1, chloroplastic isof... 80 2e-27 ref|XP_009587790.1| PREDICTED: pullulanase 1, chloroplastic isof... 79 2e-27 ref|XP_009587791.1| PREDICTED: pullulanase 1, chloroplastic isof... 79 2e-27 >ref|XP_007010038.1| Limit dextrinase [Theobroma cacao] gi|508726951|gb|EOY18848.1| Limit dextrinase [Theobroma cacao] Length = 965 Score = 79.3 bits (194), Expect(3) = 3e-28 Identities = 33/42 (78%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K+LD DSYN GDWFNRLDFTY+SN WGVG PP+EKN NWPL Sbjct: 766 KSLDRDSYNSGDWFNRLDFTYHSNNWGVGLPPKEKNEKNWPL 807 Score = 68.9 bits (167), Expect(3) = 3e-28 Identities = 33/46 (71%), Positives = 38/46 (82%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 +QP LAD FKP+ +HILAAV NF N+LRI YSSPLF L+TANAIQ Sbjct: 808 IQPRLADPSFKPQRSHILAAVENFMNVLRIRYSSPLFRLRTANAIQ 853 Score = 25.4 bits (54), Expect(3) = 3e-28 Identities = 12/20 (60%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L+ G FH+GD MLRSK L Sbjct: 749 LSQGIPFFHAGDEMLRSKSL 768 Score = 92.0 bits (227), Expect(2) = 1e-23 Identities = 51/105 (48%), Positives = 59/105 (56%), Gaps = 30/105 (28%) Frame = -2 Query: 665 EDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPGS 576 E NGVW TKGPK R LSSD +RTLFVN S Sbjct: 244 ETNGVWSTKGPKRWEGCYYVYEVSVYHPSTLQIEKCYANDPYARGLSSDGRRTLFVNLDS 303 Query: 575 DTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441 + LKPEGWD LAD+KPD+LS DISIYELH+ DFSA+D+ + F Sbjct: 304 NGLKPEGWDELADKKPDILSFSDISIYELHIRDFSANDNTVNPDF 348 Score = 45.8 bits (107), Expect(2) = 1e-23 Identities = 24/41 (58%), Positives = 28/41 (68%) Frame = -1 Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV 676 PLGA Y + VSL+LWAPTA Q V A IYKD +P EI+ Sbjct: 201 PLGAHYLGEVVSLYLWAPTA-QAVHAHIYKDPMGGSPLEII 240 >ref|XP_007220271.1| hypothetical protein PRUPE_ppa000905mg [Prunus persica] gi|462416733|gb|EMJ21470.1| hypothetical protein PRUPE_ppa000905mg [Prunus persica] Length = 965 Score = 77.4 bits (189), Expect(3) = 5e-28 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K+LD DSYN GDWFNRLDFTY+SN WGVG PP+EKN +WPL Sbjct: 772 KSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEKSWPL 813 Score = 69.3 bits (168), Expect(3) = 5e-28 Identities = 33/45 (73%), Positives = 39/45 (86%) Frame = -2 Query: 164 QPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 +P LAD FKP+++HI+AAV NFSNLLRI YSSPLF L+TANAIQ Sbjct: 815 KPRLADPSFKPQKSHIIAAVENFSNLLRIRYSSPLFRLRTANAIQ 859 Score = 26.2 bits (56), Expect(3) = 5e-28 Identities = 13/20 (65%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 LA G FHSGD +LRSK L Sbjct: 755 LAQGIPFFHSGDEILRSKSL 774 Score = 92.0 bits (227), Expect(2) = 4e-27 Identities = 50/105 (47%), Positives = 57/105 (54%), Gaps = 30/105 (28%) Frame = -2 Query: 665 EDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPGS 576 E NGVW TKGPK R LSSD +RTL VN S Sbjct: 250 EFNGVWSTKGPKSWEGCYYVYEVSVYHPSTLRIEKCYANDPYARGLSSDGRRTLLVNLDS 309 Query: 575 DTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441 D +KPEGWD L D+KPD+LS DISIYELH+ DFSASD + +F Sbjct: 310 DNIKPEGWDKLVDEKPDILSFSDISIYELHIRDFSASDQAVHPEF 354 Score = 57.8 bits (138), Expect(2) = 4e-27 Identities = 29/44 (65%), Positives = 35/44 (79%) Frame = -1 Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667 PLGALYS ++VSL+LWAPTA QEV CIYK+ S +P E+V LE Sbjct: 207 PLGALYSKQSVSLYLWAPTA-QEVCVCIYKEPSGGSPLEVVQLE 249 >ref|XP_010267258.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Nelumbo nucifera] Length = 958 Score = 78.6 bits (192), Expect(3) = 6e-28 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K+LD DSYN GDWFNRLDF+YN+N WGVG PP+EKN NWPL Sbjct: 765 KSLDRDSYNSGDWFNRLDFSYNTNNWGVGLPPKEKNEKNWPL 806 Score = 68.6 bits (166), Expect(3) = 6e-28 Identities = 32/46 (69%), Positives = 39/46 (84%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 ++P LAD FKP++ HILAAV NFSN+LRI YSSPLF L+TAN+IQ Sbjct: 807 IKPRLADPSFKPQKRHILAAVENFSNMLRIRYSSPLFRLRTANSIQ 852 Score = 25.4 bits (54), Expect(3) = 6e-28 Identities = 12/20 (60%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L+ G FHSGD +LRSK L Sbjct: 748 LSQGIPFFHSGDELLRSKSL 767 Score = 80.5 bits (197), Expect(2) = 2e-20 Identities = 39/65 (60%), Positives = 45/65 (69%) Frame = -2 Query: 635 PKDRRLSSDAQRTLFVNPGSDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHV 456 P R LSSD +RTL VN D LKP+GWD LAD+KP + S DISIYELH+ DFSASD Sbjct: 284 PYARGLSSDGKRTLLVNLDCDALKPDGWDALADEKPKLSSFSDISIYELHIRDFSASDQT 343 Query: 455 METKF 441 + F Sbjct: 344 VHPDF 348 Score = 46.6 bits (109), Expect(2) = 2e-20 Identities = 26/44 (59%), Positives = 31/44 (70%) Frame = -1 Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667 PLGA+++ +AVSL+LWAPTA Q V A IY D S P E V LE Sbjct: 201 PLGAVFTKEAVSLYLWAPTA-QAVSAIIYGDSSGGVPLESVQLE 243 >ref|XP_010267259.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Nelumbo nucifera] Length = 941 Score = 78.6 bits (192), Expect(3) = 6e-28 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K+LD DSYN GDWFNRLDF+YN+N WGVG PP+EKN NWPL Sbjct: 748 KSLDRDSYNSGDWFNRLDFSYNTNNWGVGLPPKEKNEKNWPL 789 Score = 68.6 bits (166), Expect(3) = 6e-28 Identities = 32/46 (69%), Positives = 39/46 (84%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 ++P LAD FKP++ HILAAV NFSN+LRI YSSPLF L+TAN+IQ Sbjct: 790 IKPRLADPSFKPQKRHILAAVENFSNMLRIRYSSPLFRLRTANSIQ 835 Score = 25.4 bits (54), Expect(3) = 6e-28 Identities = 12/20 (60%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L+ G FHSGD +LRSK L Sbjct: 731 LSQGIPFFHSGDELLRSKSL 750 Score = 80.5 bits (197), Expect(2) = 2e-20 Identities = 39/65 (60%), Positives = 45/65 (69%) Frame = -2 Query: 635 PKDRRLSSDAQRTLFVNPGSDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHV 456 P R LSSD +RTL VN D LKP+GWD LAD+KP + S DISIYELH+ DFSASD Sbjct: 284 PYARGLSSDGKRTLLVNLDCDALKPDGWDALADEKPKLSSFSDISIYELHIRDFSASDQT 343 Query: 455 METKF 441 + F Sbjct: 344 VHPDF 348 Score = 46.6 bits (109), Expect(2) = 2e-20 Identities = 26/44 (59%), Positives = 31/44 (70%) Frame = -1 Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667 PLGA+++ +AVSL+LWAPTA Q V A IY D S P E V LE Sbjct: 201 PLGAVFTKEAVSLYLWAPTA-QAVSAIIYGDSSGGVPLESVQLE 243 >gb|KHG14018.1| Pullulanase 1, chloroplastic -like protein [Gossypium arboreum] Length = 1016 Score = 77.8 bits (190), Expect(3) = 8e-28 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K+LD DSYN GDWFNRLDFTYNSN WGVG PP++KN +WPL Sbjct: 802 KSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPKQKNENSWPL 843 Score = 69.3 bits (168), Expect(3) = 8e-28 Identities = 33/47 (70%), Positives = 40/47 (85%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQL 27 +QP LAD FKP+ +HILAAV NF+++LRI YSSPLF L+TANAIQL Sbjct: 844 IQPRLADPSFKPQRSHILAAVENFTDVLRIRYSSPLFRLRTANAIQL 890 Score = 25.0 bits (53), Expect(3) = 8e-28 Identities = 12/20 (60%), Positives = 13/20 (65%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L G FH+GD MLRSK L Sbjct: 785 LTQGIPFFHAGDEMLRSKSL 804 Score = 86.7 bits (213), Expect(2) = 6e-23 Identities = 48/105 (45%), Positives = 56/105 (53%), Gaps = 30/105 (28%) Frame = -2 Query: 665 EDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPGS 576 E NGVW TKGPK R LS+D +RTLFVN + Sbjct: 242 ETNGVWSTKGPKSWEGCYYMYEISVYHPSTLHIEKCYANDPYARGLSADGKRTLFVNLHA 301 Query: 575 DTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441 D LKPEGW+ LAD K D+LS DISIYELH+ DFSA+D + F Sbjct: 302 DDLKPEGWNELADMKSDILSFSDISIYELHIRDFSANDDTVNADF 346 Score = 48.9 bits (115), Expect(2) = 6e-23 Identities = 28/44 (63%), Positives = 31/44 (70%) Frame = -1 Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667 PLGA YS +AVSL LWAPTA Q V A IYKD +P EI+ LE Sbjct: 199 PLGAFYSAEAVSLCLWAPTA-QAVYAHIYKDPVGGSPLEIIPLE 241 >ref|XP_002271820.3| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Vitis vinifera] gi|296085954|emb|CBI31395.3| unnamed protein product [Vitis vinifera] Length = 956 Score = 79.0 bits (193), Expect(3) = 8e-28 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K++D DSYN GDWFNRLDF+YNSN WGVG PP+EKN NWPL Sbjct: 763 KSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEKNWPL 804 Score = 67.4 bits (163), Expect(3) = 8e-28 Identities = 31/46 (67%), Positives = 39/46 (84%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 ++P LAD FKP++NHI+AAV NF NLL+I YSSPLF L+TAN+IQ Sbjct: 805 IKPRLADPSFKPQKNHIIAAVENFLNLLKIRYSSPLFRLRTANSIQ 850 Score = 25.8 bits (55), Expect(3) = 8e-28 Identities = 12/20 (60%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L+ G FHSGD MLRSK + Sbjct: 746 LSQGIPFFHSGDEMLRSKSI 765 Score = 89.0 bits (219), Expect(2) = 2e-25 Identities = 50/105 (47%), Positives = 56/105 (53%), Gaps = 30/105 (28%) Frame = -2 Query: 665 EDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPGS 576 E NGVW GPK R LS+DA+RTL VN S Sbjct: 241 EFNGVWSASGPKSWEGCYYEYEVSVYHPSTLQIEKCTANDPYARGLSADARRTLLVNLDS 300 Query: 575 DTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441 D LKPEGWD LAD+KP +LS DISIYELH+ DFSASDH + F Sbjct: 301 DDLKPEGWDNLADEKPALLSFSDISIYELHIRDFSASDHTVHPDF 345 Score = 54.7 bits (130), Expect(2) = 2e-25 Identities = 28/44 (63%), Positives = 34/44 (77%) Frame = -1 Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667 PLGA++S +AV+L+LWAPTA Q V ACIY D S +P EIV LE Sbjct: 198 PLGAIFSKEAVTLYLWAPTA-QVVHACIYGDPSGGDPLEIVCLE 240 >ref|XP_010658333.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Vitis vinifera] Length = 954 Score = 79.0 bits (193), Expect(3) = 8e-28 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K++D DSYN GDWFNRLDF+YNSN WGVG PP+EKN NWPL Sbjct: 761 KSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEKNWPL 802 Score = 67.4 bits (163), Expect(3) = 8e-28 Identities = 31/46 (67%), Positives = 39/46 (84%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 ++P LAD FKP++NHI+AAV NF NLL+I YSSPLF L+TAN+IQ Sbjct: 803 IKPRLADPSFKPQKNHIIAAVENFLNLLKIRYSSPLFRLRTANSIQ 848 Score = 25.8 bits (55), Expect(3) = 8e-28 Identities = 12/20 (60%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L+ G FHSGD MLRSK + Sbjct: 744 LSQGIPFFHSGDEMLRSKSI 763 Score = 89.0 bits (219), Expect(2) = 2e-25 Identities = 50/105 (47%), Positives = 56/105 (53%), Gaps = 30/105 (28%) Frame = -2 Query: 665 EDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPGS 576 E NGVW GPK R LS+DA+RTL VN S Sbjct: 239 EFNGVWSASGPKSWEGCYYEYEVSVYHPSTLQIEKCTANDPYARGLSADARRTLLVNLDS 298 Query: 575 DTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441 D LKPEGWD LAD+KP +LS DISIYELH+ DFSASDH + F Sbjct: 299 DDLKPEGWDNLADEKPALLSFSDISIYELHIRDFSASDHTVHPDF 343 Score = 54.7 bits (130), Expect(2) = 2e-25 Identities = 28/44 (63%), Positives = 34/44 (77%) Frame = -1 Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667 PLGA++S +AV+L+LWAPTA Q V ACIY D S +P EIV LE Sbjct: 196 PLGAIFSKEAVTLYLWAPTA-QVVHACIYGDPSGGDPLEIVCLE 238 >ref|XP_008233250.1| PREDICTED: pullulanase 1, chloroplastic [Prunus mume] Length = 967 Score = 92.4 bits (228), Expect(2) = 1e-27 Identities = 50/105 (47%), Positives = 58/105 (55%), Gaps = 30/105 (28%) Frame = -2 Query: 665 EDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPGS 576 E NGVW TKGPK R LSSD++RTL VN S Sbjct: 250 EFNGVWSTKGPKSWEGCYYVYEVSVYHPSTLRIEKCYANDPYARGLSSDSRRTLLVNLDS 309 Query: 575 DTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441 D +KPEGWD L D+KPD+LS DISIYELH+ DFSASD + +F Sbjct: 310 DNIKPEGWDKLVDEKPDILSFSDISIYELHIRDFSASDQAVHPEF 354 Score = 59.3 bits (142), Expect(2) = 1e-27 Identities = 30/44 (68%), Positives = 35/44 (79%) Frame = -1 Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667 PLGALYS ++VSL+LWAPTA QEV CIYKD S +P E+V LE Sbjct: 207 PLGALYSKQSVSLYLWAPTA-QEVCVCIYKDPSGGSPLEVVQLE 249 Score = 77.4 bits (189), Expect(3) = 2e-27 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K+LD DSYN GDWFNRLDFTY+SN WGVG PP+EKN +WPL Sbjct: 774 KSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEKSWPL 815 Score = 67.0 bits (162), Expect(3) = 2e-27 Identities = 32/45 (71%), Positives = 37/45 (82%) Frame = -2 Query: 164 QPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 +P LAD FKP+ +HI+AAV NFSNLL I YSSPLF L+TANAIQ Sbjct: 817 KPRLADPSFKPQRSHIIAAVENFSNLLHIRYSSPLFRLRTANAIQ 861 Score = 26.2 bits (56), Expect(3) = 2e-27 Identities = 13/20 (65%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 LA G FHSGD +LRSK L Sbjct: 757 LAQGIPFFHSGDEILRSKSL 776 >ref|XP_009368969.1| PREDICTED: pullulanase 1, chloroplastic-like isoform X1 [Pyrus x bretschneideri] Length = 978 Score = 77.0 bits (188), Expect(3) = 1e-27 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K+LD DSYN GDWFNRLDFTY+SN WGVG PP+EKN +WPL Sbjct: 785 KSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEHSWPL 826 Score = 70.9 bits (172), Expect(3) = 1e-27 Identities = 34/46 (73%), Positives = 40/46 (86%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 ++P LAD FKP+++HILAAV NFSNLLRI YSSPLF L+TANAIQ Sbjct: 827 MKPRLADPSFKPQKSHILAAVENFSNLLRIRYSSPLFRLRTANAIQ 872 Score = 23.9 bits (50), Expect(3) = 1e-27 Identities = 11/20 (55%), Positives = 13/20 (65%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L G FH+GD +LRSK L Sbjct: 768 LGQGIPFFHAGDELLRSKSL 787 Score = 90.1 bits (222), Expect(2) = 2e-26 Identities = 49/105 (46%), Positives = 58/105 (55%), Gaps = 30/105 (28%) Frame = -2 Query: 665 EDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPGS 576 E NGVW TKGPK R LSSD +RTL VN S Sbjct: 263 EVNGVWSTKGPKSWEGCYYVYEVSVYHPSTLKIEKCYANDPYARGLSSDGRRTLLVNLDS 322 Query: 575 DTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441 D +KPEGWD LAD+KPD+ S DISIYELH+ DFSA+D + ++F Sbjct: 323 DDIKPEGWDKLADEKPDINSFSDISIYELHIRDFSANDQTVHSEF 367 Score = 57.4 bits (137), Expect(2) = 2e-26 Identities = 30/45 (66%), Positives = 36/45 (80%), Gaps = 1/45 (2%) Frame = -1 Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKD-VSADNPQEIV*LE 667 PLGALYS ++VSL+LWAPTA QEV CIYK+ + NPQE+V LE Sbjct: 219 PLGALYSEESVSLYLWAPTA-QEVCVCIYKEPLGGGNPQEVVQLE 262 >ref|XP_009368970.1| PREDICTED: pullulanase 1, chloroplastic-like isoform X2 [Pyrus x bretschneideri] Length = 977 Score = 77.0 bits (188), Expect(3) = 1e-27 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K+LD DSYN GDWFNRLDFTY+SN WGVG PP+EKN +WPL Sbjct: 784 KSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEHSWPL 825 Score = 70.9 bits (172), Expect(3) = 1e-27 Identities = 34/46 (73%), Positives = 40/46 (86%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 ++P LAD FKP+++HILAAV NFSNLLRI YSSPLF L+TANAIQ Sbjct: 826 MKPRLADPSFKPQKSHILAAVENFSNLLRIRYSSPLFRLRTANAIQ 871 Score = 23.9 bits (50), Expect(3) = 1e-27 Identities = 11/20 (55%), Positives = 13/20 (65%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L G FH+GD +LRSK L Sbjct: 767 LGQGIPFFHAGDELLRSKSL 786 Score = 90.1 bits (222), Expect(2) = 2e-26 Identities = 49/105 (46%), Positives = 58/105 (55%), Gaps = 30/105 (28%) Frame = -2 Query: 665 EDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPGS 576 E NGVW TKGPK R LSSD +RTL VN S Sbjct: 262 EVNGVWSTKGPKSWEGCYYVYEVSVYHPSTLKIEKCYANDPYARGLSSDGRRTLLVNLDS 321 Query: 575 DTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441 D +KPEGWD LAD+KPD+ S DISIYELH+ DFSA+D + ++F Sbjct: 322 DDIKPEGWDKLADEKPDINSFSDISIYELHIRDFSANDQTVHSEF 366 Score = 57.4 bits (137), Expect(2) = 2e-26 Identities = 30/45 (66%), Positives = 36/45 (80%), Gaps = 1/45 (2%) Frame = -1 Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKD-VSADNPQEIV*LE 667 PLGALYS ++VSL+LWAPTA QEV CIYK+ + NPQE+V LE Sbjct: 218 PLGALYSEESVSLYLWAPTA-QEVCVCIYKEPLGGGNPQEVVQLE 261 >ref|XP_011024340.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Populus euphratica] Length = 971 Score = 78.2 bits (191), Expect(3) = 1e-27 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K+LD DSYN GDWFNRLDFTYNSN WGVG PP++KN +WPL Sbjct: 780 KSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPKQKNEKHWPL 821 Score = 66.6 bits (161), Expect(3) = 1e-27 Identities = 31/46 (67%), Positives = 39/46 (84%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 ++P LAD FKP++NHILAA+NNF ++L+I YSSPLF L TANAIQ Sbjct: 822 IRPRLADPSFKPQKNHILAAINNFLDVLQIRYSSPLFRLTTANAIQ 867 Score = 26.6 bits (57), Expect(3) = 1e-27 Identities = 13/20 (65%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L+ G FHSGD MLRSK L Sbjct: 763 LSQGIPFFHSGDEMLRSKSL 782 Score = 91.7 bits (226), Expect = 6e-16 Identities = 51/106 (48%), Positives = 59/106 (55%), Gaps = 30/106 (28%) Frame = -2 Query: 668 REDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPG 579 +E NGVW +G KD R LS D+QRTLFVN Sbjct: 257 KEVNGVWSVEGSKDWEGCYYVYEVNVYHPSTLRVEKCYANDPYARGLSPDSQRTLFVNLD 316 Query: 578 SDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441 SDTLKPEGW+ LAD+KP +LS DISIYELHV DFSA+DH + F Sbjct: 317 SDTLKPEGWEKLADEKPIILSFSDISIYELHVRDFSANDHTVHPDF 362 >ref|XP_011024341.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Populus euphratica] Length = 969 Score = 78.2 bits (191), Expect(3) = 1e-27 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K+LD DSYN GDWFNRLDFTYNSN WGVG PP++KN +WPL Sbjct: 778 KSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPKQKNEKHWPL 819 Score = 66.6 bits (161), Expect(3) = 1e-27 Identities = 31/46 (67%), Positives = 39/46 (84%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 ++P LAD FKP++NHILAA+NNF ++L+I YSSPLF L TANAIQ Sbjct: 820 IRPRLADPSFKPQKNHILAAINNFLDVLQIRYSSPLFRLTTANAIQ 865 Score = 26.6 bits (57), Expect(3) = 1e-27 Identities = 13/20 (65%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L+ G FHSGD MLRSK L Sbjct: 761 LSQGIPFFHSGDEMLRSKSL 780 Score = 91.7 bits (226), Expect = 6e-16 Identities = 51/106 (48%), Positives = 59/106 (55%), Gaps = 30/106 (28%) Frame = -2 Query: 668 REDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPG 579 +E NGVW +G KD R LS D+QRTLFVN Sbjct: 255 KEVNGVWSVEGSKDWEGCYYVYEVNVYHPSTLRVEKCYANDPYARGLSPDSQRTLFVNLD 314 Query: 578 SDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441 SDTLKPEGW+ LAD+KP +LS DISIYELHV DFSA+DH + F Sbjct: 315 SDTLKPEGWEKLADEKPIILSFSDISIYELHVRDFSANDHTVHPDF 360 >ref|XP_006378784.1| hypothetical protein POPTR_0010s23600g [Populus trichocarpa] gi|550330451|gb|ERP56581.1| hypothetical protein POPTR_0010s23600g [Populus trichocarpa] Length = 945 Score = 78.2 bits (191), Expect(3) = 1e-27 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K+LD DSYN GDWFNRLDFTYNSN WGVG PP++KN +WPL Sbjct: 780 KSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPKQKNEKHWPL 821 Score = 66.6 bits (161), Expect(3) = 1e-27 Identities = 31/46 (67%), Positives = 39/46 (84%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 ++P LAD FKP++NHILAA+NNF ++L+I YSSPLF L TANAIQ Sbjct: 822 IRPRLADPSFKPQKNHILAAINNFLDVLQIRYSSPLFRLTTANAIQ 867 Score = 26.6 bits (57), Expect(3) = 1e-27 Identities = 13/20 (65%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L+ G FHSGD MLRSK L Sbjct: 763 LSQGIPFFHSGDEMLRSKSL 782 Score = 91.7 bits (226), Expect = 6e-16 Identities = 51/106 (48%), Positives = 59/106 (55%), Gaps = 30/106 (28%) Frame = -2 Query: 668 REDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPG 579 +E NGVW +G KD R LS D+QRTLFVN Sbjct: 257 KEVNGVWSVEGSKDWEGCYYVYEVSVYHPSTLHVEKCYANDPYARGLSPDSQRTLFVNLD 316 Query: 578 SDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441 SDTLKPEGW+ LAD+KP +LS DISIYELHV DFSA+DH + F Sbjct: 317 SDTLKPEGWEKLADEKPIILSFSDISIYELHVRDFSANDHTVHPDF 362 >ref|XP_002315334.2| pullulanase family protein [Populus trichocarpa] gi|550330450|gb|EEF01505.2| pullulanase family protein [Populus trichocarpa] Length = 943 Score = 78.2 bits (191), Expect(3) = 1e-27 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K+LD DSYN GDWFNRLDFTYNSN WGVG PP++KN +WPL Sbjct: 778 KSLDRDSYNSGDWFNRLDFTYNSNNWGVGLPPKQKNEKHWPL 819 Score = 66.6 bits (161), Expect(3) = 1e-27 Identities = 31/46 (67%), Positives = 39/46 (84%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 ++P LAD FKP++NHILAA+NNF ++L+I YSSPLF L TANAIQ Sbjct: 820 IRPRLADPSFKPQKNHILAAINNFLDVLQIRYSSPLFRLTTANAIQ 865 Score = 26.6 bits (57), Expect(3) = 1e-27 Identities = 13/20 (65%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L+ G FHSGD MLRSK L Sbjct: 761 LSQGIPFFHSGDEMLRSKSL 780 Score = 91.7 bits (226), Expect = 6e-16 Identities = 51/106 (48%), Positives = 59/106 (55%), Gaps = 30/106 (28%) Frame = -2 Query: 668 REDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPG 579 +E NGVW +G KD R LS D+QRTLFVN Sbjct: 255 KEVNGVWSVEGSKDWEGCYYVYEVSVYHPSTLHVEKCYANDPYARGLSPDSQRTLFVNLD 314 Query: 578 SDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441 SDTLKPEGW+ LAD+KP +LS DISIYELHV DFSA+DH + F Sbjct: 315 SDTLKPEGWEKLADEKPIILSFSDISIYELHVRDFSANDHTVHPDF 360 >ref|XP_009587793.1| PREDICTED: pullulanase 1, chloroplastic isoform X5 [Nicotiana tomentosiformis] gi|697158090|ref|XP_009587794.1| PREDICTED: pullulanase 1, chloroplastic isoform X5 [Nicotiana tomentosiformis] Length = 849 Score = 79.0 bits (193), Expect(3) = 1e-27 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K++D DSYN GDWFNRLDF+YNSN WGVG PP+EKN NWPL Sbjct: 761 KSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPL 802 Score = 66.6 bits (161), Expect(3) = 1e-27 Identities = 30/47 (63%), Positives = 41/47 (87%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQL 27 ++P LAD +KP+++HILAAV NFSNL++I YSSPLF L+TAN+IQ+ Sbjct: 803 IKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQV 849 Score = 25.8 bits (55), Expect(3) = 1e-27 Identities = 12/20 (60%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L+ G FHSGD MLRSK + Sbjct: 744 LSQGIPFFHSGDEMLRSKSI 763 Score = 79.0 bits (193), Expect = 4e-12 Identities = 44/106 (41%), Positives = 56/106 (52%), Gaps = 30/106 (28%) Frame = -2 Query: 668 REDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPG 579 +E NGVW KGP+ R LS+D +R L VN Sbjct: 238 KELNGVWSVKGPRHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRALLVNLD 297 Query: 578 SDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441 SD +KPEGWD L D+KP++LS DIS+YELHV DFSA+D + +F Sbjct: 298 SDDVKPEGWDNLQDEKPNLLSFSDISLYELHVRDFSANDPTVPHEF 343 >ref|XP_009791833.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Nicotiana sylvestris] Length = 952 Score = 79.0 bits (193), Expect(3) = 2e-27 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K++D DSYN GDWFNRLDF+YNSN WGVG PP+EKN NWPL Sbjct: 761 KSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPL 802 Score = 66.2 bits (160), Expect(3) = 2e-27 Identities = 30/46 (65%), Positives = 40/46 (86%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 ++P LAD +KP+++HILAAV NFSNL++I YSSPLF L+TAN+IQ Sbjct: 803 IKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQ 848 Score = 25.8 bits (55), Expect(3) = 2e-27 Identities = 12/20 (60%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L+ G FHSGD MLRSK + Sbjct: 744 LSQGIPFFHSGDEMLRSKSI 763 Score = 80.1 bits (196), Expect(2) = 5e-21 Identities = 39/65 (60%), Positives = 48/65 (73%) Frame = -2 Query: 635 PKDRRLSSDAQRTLFVNPGSDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHV 456 P R LS+D +RTL VN SD +KPEGWD L D+KPD+LS DIS+YELHV DFSA+D Sbjct: 279 PYARGLSADGKRTLLVNLVSDDVKPEGWDNLQDEKPDLLSFSDISLYELHVRDFSANDPT 338 Query: 455 METKF 441 + +F Sbjct: 339 VPHEF 343 Score = 48.9 bits (115), Expect(2) = 5e-21 Identities = 27/44 (61%), Positives = 32/44 (72%) Frame = -1 Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667 PLGA+ +AVSL+LWAPTA Q VRA IYK S +P EIV L+ Sbjct: 196 PLGAVCDTEAVSLYLWAPTA-QAVRALIYKSPSETDPVEIVQLK 238 >ref|XP_009587786.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Nicotiana tomentosiformis] gi|697158080|ref|XP_009587789.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Nicotiana tomentosiformis] Length = 952 Score = 79.0 bits (193), Expect(3) = 2e-27 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K++D DSYN GDWFNRLDF+YNSN WGVG PP+EKN NWPL Sbjct: 761 KSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPL 802 Score = 66.2 bits (160), Expect(3) = 2e-27 Identities = 30/46 (65%), Positives = 40/46 (86%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 ++P LAD +KP+++HILAAV NFSNL++I YSSPLF L+TAN+IQ Sbjct: 803 IKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQ 848 Score = 25.8 bits (55), Expect(3) = 2e-27 Identities = 12/20 (60%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L+ G FHSGD MLRSK + Sbjct: 744 LSQGIPFFHSGDEMLRSKSI 763 Score = 79.0 bits (193), Expect = 4e-12 Identities = 44/106 (41%), Positives = 56/106 (52%), Gaps = 30/106 (28%) Frame = -2 Query: 668 REDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPG 579 +E NGVW KGP+ R LS+D +R L VN Sbjct: 238 KELNGVWSVKGPRHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRALLVNLD 297 Query: 578 SDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441 SD +KPEGWD L D+KP++LS DIS+YELHV DFSA+D + +F Sbjct: 298 SDDVKPEGWDNLQDEKPNLLSFSDISLYELHVRDFSANDPTVPHEF 343 >ref|XP_009791834.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Nicotiana sylvestris] Length = 927 Score = 79.0 bits (193), Expect(3) = 2e-27 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K++D DSYN GDWFNRLDF+YNSN WGVG PP+EKN NWPL Sbjct: 761 KSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPL 802 Score = 66.2 bits (160), Expect(3) = 2e-27 Identities = 30/46 (65%), Positives = 40/46 (86%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 ++P LAD +KP+++HILAAV NFSNL++I YSSPLF L+TAN+IQ Sbjct: 803 IKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQ 848 Score = 25.8 bits (55), Expect(3) = 2e-27 Identities = 12/20 (60%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L+ G FHSGD MLRSK + Sbjct: 744 LSQGIPFFHSGDEMLRSKSI 763 Score = 80.1 bits (196), Expect(2) = 5e-21 Identities = 39/65 (60%), Positives = 48/65 (73%) Frame = -2 Query: 635 PKDRRLSSDAQRTLFVNPGSDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHV 456 P R LS+D +RTL VN SD +KPEGWD L D+KPD+LS DIS+YELHV DFSA+D Sbjct: 279 PYARGLSADGKRTLLVNLVSDDVKPEGWDNLQDEKPDLLSFSDISLYELHVRDFSANDPT 338 Query: 455 METKF 441 + +F Sbjct: 339 VPHEF 343 Score = 48.9 bits (115), Expect(2) = 5e-21 Identities = 27/44 (61%), Positives = 32/44 (72%) Frame = -1 Query: 798 PLGALYSNKAVSLFLWAPTALQEVRACIYKDVSADNPQEIV*LE 667 PLGA+ +AVSL+LWAPTA Q VRA IYK S +P EIV L+ Sbjct: 196 PLGAVCDTEAVSLYLWAPTA-QAVRALIYKSPSETDPVEIVQLK 238 >ref|XP_009587790.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Nicotiana tomentosiformis] Length = 927 Score = 79.0 bits (193), Expect(3) = 2e-27 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K++D DSYN GDWFNRLDF+YNSN WGVG PP+EKN NWPL Sbjct: 761 KSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPL 802 Score = 66.2 bits (160), Expect(3) = 2e-27 Identities = 30/46 (65%), Positives = 40/46 (86%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 ++P LAD +KP+++HILAAV NFSNL++I YSSPLF L+TAN+IQ Sbjct: 803 IKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQ 848 Score = 25.8 bits (55), Expect(3) = 2e-27 Identities = 12/20 (60%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L+ G FHSGD MLRSK + Sbjct: 744 LSQGIPFFHSGDEMLRSKSI 763 Score = 79.0 bits (193), Expect = 4e-12 Identities = 44/106 (41%), Positives = 56/106 (52%), Gaps = 30/106 (28%) Frame = -2 Query: 668 REDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPG 579 +E NGVW KGP+ R LS+D +R L VN Sbjct: 238 KELNGVWSVKGPRHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRALLVNLD 297 Query: 578 SDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441 SD +KPEGWD L D+KP++LS DIS+YELHV DFSA+D + +F Sbjct: 298 SDDVKPEGWDNLQDEKPNLLSFSDISLYELHVRDFSANDPTVPHEF 343 >ref|XP_009587791.1| PREDICTED: pullulanase 1, chloroplastic isoform X3 [Nicotiana tomentosiformis] Length = 920 Score = 79.0 bits (193), Expect(3) = 2e-27 Identities = 32/42 (76%), Positives = 36/42 (85%) Frame = -3 Query: 307 KTLDSDSYNCGDWFNRLDFTYNSNYWGVGHPPEEKNGMNWPL 182 K++D DSYN GDWFNRLDF+YNSN WGVG PP+EKN NWPL Sbjct: 729 KSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPL 770 Score = 66.2 bits (160), Expect(3) = 2e-27 Identities = 30/46 (65%), Positives = 40/46 (86%) Frame = -2 Query: 167 LQPGLADQLFKPKENHILAAVNNFSNLLRISYSSPLFHLKTANAIQ 30 ++P LAD +KP+++HILAAV NFSNL++I YSSPLF L+TAN+IQ Sbjct: 771 IKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQ 816 Score = 25.8 bits (55), Expect(3) = 2e-27 Identities = 12/20 (60%), Positives = 14/20 (70%) Frame = -1 Query: 357 LAPGNIVFHSGDGMLRSKHL 298 L+ G FHSGD MLRSK + Sbjct: 712 LSQGIPFFHSGDEMLRSKSI 731 Score = 79.0 bits (193), Expect = 4e-12 Identities = 44/106 (41%), Positives = 56/106 (52%), Gaps = 30/106 (28%) Frame = -2 Query: 668 REDNGVWKTKGPKD------------------------------RRLSSDAQRTLFVNPG 579 +E NGVW KGP+ R LS+D +R L VN Sbjct: 238 KELNGVWSVKGPRHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRALLVNLD 297 Query: 578 SDTLKPEGWDILADQKPDVLSLLDISIYELHVMDFSASDHVMETKF 441 SD +KPEGWD L D+KP++LS DIS+YELHV DFSA+D + +F Sbjct: 298 SDDVKPEGWDNLQDEKPNLLSFSDISLYELHVRDFSANDPTVPHEF 343