BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ziziphus21_contig00009599
         (1746 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_008238179.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   803   0.0  
ref|XP_010102337.1| tRNA-specific 2-thiouridylase mnmA [Morus no...   799   0.0  
ref|XP_007209892.1| hypothetical protein PRUPE_ppa004048mg [Prun...   786   0.0  
ref|XP_002282827.2| PREDICTED: mitochondrial tRNA-specific 2-thi...   783   0.0  
ref|XP_009376883.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   779   0.0  
ref|XP_009347315.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   775   0.0  
ref|XP_009376884.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   773   0.0  
ref|XP_008385559.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   766   0.0  
ref|XP_008385560.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   766   0.0  
ref|XP_008344848.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   762   0.0  
ref|XP_009375052.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   761   0.0  
ref|XP_009375053.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   761   0.0  
ref|XP_009375051.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   761   0.0  
ref|XP_006470603.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   758   0.0  
gb|KDO61266.1| hypothetical protein CISIN_1g042717mg, partial [C...   757   0.0  
ref|XP_008449720.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   746   0.0  
ref|XP_012446852.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   742   0.0  
ref|XP_004142116.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   741   0.0  
ref|XP_010257320.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   737   0.0  
ref|XP_012089993.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   736   0.0  

>ref|XP_008238179.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 isoform X1
            [Prunus mume]
          Length = 537

 Score =  803 bits (2075), Expect = 0.0
 Identities = 402/513 (78%), Positives = 435/513 (84%)
 Frame = -2

Query: 1634 IPLKPHVPFFHNRTSLFLRPLXXXXXXXXXXAELLTPNSIESFNISLQPYLSCCMPHKRF 1455
            + L PH P  +   +LFLRPL           +  TP+   S N  L PYLSC MPH+  
Sbjct: 26   LSLFPHSP--NPARTLFLRPLSRCCSSSSAVPQ--TPHPPNSINGDLGPYLSCSMPHQSL 81

Query: 1454 RVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYAKAVCS 1275
            +VAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYAKAVC+
Sbjct: 82   KVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYAKAVCN 141

Query: 1274 QVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTRIKFGAFMDAISKMDFDYV 1095
            QVDVPLEVVHLT+EYW+NVVSYII+EYRCGRTPNPDVLCNTRIKFGAFMDAIS M FDYV
Sbjct: 142  QVDVPLEVVHLTNEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISCMKFDYV 201

Query: 1094 ASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQAQLKRLIFPLGCVSKEEVR 915
            ASGHYANV+H S  + D  SVLELS DMVKDQTYFL+HLSQAQLK+LIFPLGC+SK+EVR
Sbjct: 202  ASGHYANVVHPSAGEMDKDSVLELSQDMVKDQTYFLSHLSQAQLKQLIFPLGCLSKDEVR 261

Query: 914  KLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGIILEAETGDLLGKHRGFWFY 735
            KLATKFDL NK RKDSQGICFLGKIKF+EF+AR IGEKEGIILEAETGDLLG HRGFWFY
Sbjct: 262  KLATKFDLLNKQRKDSQGICFLGKIKFSEFIARHIGEKEGIILEAETGDLLGTHRGFWFY 321

Query: 734  TIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRLFRVGSLKWLSGLPPNHIS 555
            TIGQRQGLRLPGGPWYVV KD++NNVVFVSRNYFS DKRRRLFRVGSLKWL+G PPN IS
Sbjct: 322  TIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSVDKRRRLFRVGSLKWLNGFPPNQIS 381

Query: 554  QLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLAAGQFAAFYQGKTCLGSGI 375
            QL+CKVRHGPGF NCSL ME G   HEDVAVVHLS DDQGLAAGQFAAFYQG+TCLGSG+
Sbjct: 382  QLQCKVRHGPGFRNCSLIMEPGAYGHEDVAVVHLSEDDQGLAAGQFAAFYQGRTCLGSGV 441

Query: 374  ILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKPEKTHFMEANQKGVIELNRGQSS 195
            ILESWDDQGFPVCA+ALEIARMEDKSLLG+PVKIKVKPE     E++Q   +EL  G   
Sbjct: 442  ILESWDDQGFPVCARALEIARMEDKSLLGRPVKIKVKPENP-ITESDQVDGMELCGGLKH 500

Query: 194  RSASAAIKQRSQASQGEVISRFPMKLLQKFRNK 96
               SAA KQR Q SQGE I+RFP+  LQKFR K
Sbjct: 501  SGISAA-KQRMQVSQGEAITRFPVNWLQKFREK 532


>ref|XP_010102337.1| tRNA-specific 2-thiouridylase mnmA [Morus notabilis]
            gi|587905107|gb|EXB93298.1| tRNA-specific 2-thiouridylase
            mnmA [Morus notabilis]
          Length = 580

 Score =  799 bits (2064), Expect = 0.0
 Identities = 403/538 (74%), Positives = 443/538 (82%), Gaps = 13/538 (2%)
 Frame = -2

Query: 1670 LRVVGVMAMPSS----------IPLKPHV---PFFHNRTSLFLRPLXXXXXXXXXXAELL 1530
            LR+  VM +PSS          +PLKP     P  H  TS   + L          + + 
Sbjct: 49   LRLTRVMPIPSSSLIIQSRFISLPLKPRTRLPPKPHLPTS---KSLVFRLFSVSAASAVS 105

Query: 1529 TPNSIESFNISLQPYLSCCMPHKRFRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWF 1350
            + +  +SF+  ++PYLSC MPHKR RVAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWF
Sbjct: 106  SSHHSKSFDGDIEPYLSCSMPHKRLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWF 165

Query: 1349 QEDFENFWSECPWEEDLKYAKAVCSQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNP 1170
            QEDFENFWSECPWEEDLKYAKAVCSQ++VPLEVVHLTDEYW+NVVSYIIDEYRCGRTPNP
Sbjct: 166  QEDFENFWSECPWEEDLKYAKAVCSQIEVPLEVVHLTDEYWKNVVSYIIDEYRCGRTPNP 225

Query: 1169 DVLCNTRIKFGAFMDAISKMDFDYVASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYF 990
            DVLCNTRIKFG+FMDAIS M+FDYVASGHYANV+HS  ++  + SVLELS DMVKDQTYF
Sbjct: 226  DVLCNTRIKFGSFMDAISSMEFDYVASGHYANVVHSFTNERGDASVLELSQDMVKDQTYF 285

Query: 989  LAHLSQAQLKRLIFPLGCVSKEEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQI 810
            L+HLSQAQLKRL+FPLGC+SKEEVRKLA KFDLPNKDRKDSQGICFLGKIKF+EFVAR I
Sbjct: 286  LSHLSQAQLKRLVFPLGCISKEEVRKLAKKFDLPNKDRKDSQGICFLGKIKFSEFVARHI 345

Query: 809  GEKEGIILEAETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFS 630
            GEKEGIILEAETGD LG HRGFWFYTIGQRQGLRLPGGPWYVV KDV+NNVVF SRNYFS
Sbjct: 346  GEKEGIILEAETGDFLGMHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFASRNYFS 405

Query: 629  FDKRRRLFRVGSLKWLSGLPPNHISQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLS 450
            FDKRRR+FRVGSLKWL+GLPP+   QL+CKVRHGPGFYNCS TMESG++S EDVAVV LS
Sbjct: 406  FDKRRRIFRVGSLKWLNGLPPDQTCQLQCKVRHGPGFYNCSFTMESGENSGEDVAVVQLS 465

Query: 449  GDDQGLAAGQFAAFYQGKTCLGSGIILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIK 270
             DDQGLA GQFAAFYQGK+CLGSGIILESWDDQGFPVC KALEIA+MEDKSLLGKPVKIK
Sbjct: 466  EDDQGLAVGQFAAFYQGKSCLGSGIILESWDDQGFPVCEKALEIAKMEDKSLLGKPVKIK 525

Query: 269  VKPEKTHFMEANQKGVIELNRGQSSRSASAAIKQRSQASQGEVISRFPMKLLQKFRNK 96
            VKPE +  +E  QK   EL+RGQ S   +A       A QG     FPMK LQ+ R K
Sbjct: 526  VKPE-SPTIEPGQKSSTELDRGQRSSHIAA-------AKQGRQALSFPMKWLQRLREK 575


>ref|XP_007209892.1| hypothetical protein PRUPE_ppa004048mg [Prunus persica]
            gi|462405627|gb|EMJ11091.1| hypothetical protein
            PRUPE_ppa004048mg [Prunus persica]
          Length = 533

 Score =  786 bits (2029), Expect = 0.0
 Identities = 396/513 (77%), Positives = 429/513 (83%)
 Frame = -2

Query: 1634 IPLKPHVPFFHNRTSLFLRPLXXXXXXXXXXAELLTPNSIESFNISLQPYLSCCMPHKRF 1455
            + L PH P  +   +LFL PL           +  TP+   S N  L PYLSC MPH+  
Sbjct: 26   LSLFPHSP--NPARTLFLGPLSRCCSSSSAVPQ--TPHPPNSINGDLGPYLSCSMPHQSL 81

Query: 1454 RVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYAKAVCS 1275
            +VAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYAKAVC+
Sbjct: 82   KVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYAKAVCN 141

Query: 1274 QVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTRIKFGAFMDAISKMDFDYV 1095
            QVDVPLEVVHLT+EYW+NVVSYII+EYRCGRTPNPDVLCNTRIKFGAFMDAIS M FDYV
Sbjct: 142  QVDVPLEVVHLTNEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISCMKFDYV 201

Query: 1094 ASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQAQLKRLIFPLGCVSKEEVR 915
            ASGHYANV+H S  + D  SVLELS DM    TYFL+HLSQAQLK+LIFPLGC+SK+EVR
Sbjct: 202  ASGHYANVVHPSAGEMDEDSVLELSQDM----TYFLSHLSQAQLKQLIFPLGCLSKDEVR 257

Query: 914  KLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGIILEAETGDLLGKHRGFWFY 735
            KLATKFDL NK RKDSQGICFLGK+KF+EF+AR IGEKEGIILEAETGD LG HRGFWFY
Sbjct: 258  KLATKFDLLNKQRKDSQGICFLGKLKFSEFIARHIGEKEGIILEAETGDFLGTHRGFWFY 317

Query: 734  TIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRLFRVGSLKWLSGLPPNHIS 555
            TIGQRQGLRLPGGPWYVV KD++NNVVFVSRNYFS DKRRRLFRVGSLKWL+G P N IS
Sbjct: 318  TIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSVDKRRRLFRVGSLKWLNGFPQNQIS 377

Query: 554  QLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLAAGQFAAFYQGKTCLGSGI 375
            QL+CKVRHGPGF NCSL ME G   HEDVAVVHLS DDQGLAAGQFAAFYQG+TCLGSG+
Sbjct: 378  QLQCKVRHGPGFRNCSLIMEPGAYGHEDVAVVHLSEDDQGLAAGQFAAFYQGRTCLGSGV 437

Query: 374  ILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKPEKTHFMEANQKGVIELNRGQSS 195
            ILESWDDQGFPVCA+ALEIARMEDKSLLGKPVKIKVKPE     E++Q   +EL RG   
Sbjct: 438  ILESWDDQGFPVCARALEIARMEDKSLLGKPVKIKVKPENP-ITESDQVDGMELCRGLKH 496

Query: 194  RSASAAIKQRSQASQGEVISRFPMKLLQKFRNK 96
               SAA KQR Q SQGE I+RFP+  LQKFR K
Sbjct: 497  SGISAA-KQRMQVSQGEAITRFPVNWLQKFREK 528


>ref|XP_002282827.2| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Vitis
            vinifera] gi|297746327|emb|CBI16383.3| unnamed protein
            product [Vitis vinifera]
          Length = 544

 Score =  783 bits (2023), Expect = 0.0
 Identities = 383/480 (79%), Positives = 420/480 (87%)
 Frame = -2

Query: 1535 LLTPNSIESFNISLQPYLSCCMPHKRFRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKI 1356
            + TP S   FN  LQPYLSC +P+ R ++AVL+SGGVDSSVALRLLHAAGHSCTAFYLKI
Sbjct: 63   ITTPPS-NLFNADLQPYLSCSLPNNRLKIAVLLSGGVDSSVALRLLHAAGHSCTAFYLKI 121

Query: 1355 WFQEDFENFWSECPWEEDLKYAKAVCSQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTP 1176
            WFQEDFENFWSECPWE+DLKYAKAVC QVDVPLEVVHLTDEYW+NVVSYII+EYRCGRTP
Sbjct: 122  WFQEDFENFWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTP 181

Query: 1175 NPDVLCNTRIKFGAFMDAISKMDFDYVASGHYANVIHSSRSQTDNISVLELSNDMVKDQT 996
            NPDVLCNTRIKFGAFMDAIS  +FDYVASGHYANV+HS   Q D  SVLELS DMVKDQT
Sbjct: 182  NPDVLCNTRIKFGAFMDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVKDQT 241

Query: 995  YFLAHLSQAQLKRLIFPLGCVSKEEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFVAR 816
            YFL+HLSQ QLKRLIFPLGC+ K+EVR LATK DLPN+DRKDSQGICFLGKIKF+EFVAR
Sbjct: 242  YFLSHLSQIQLKRLIFPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVAR 301

Query: 815  QIGEKEGIILEAETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNY 636
             IGEKEGIILEAETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVV KDV+NNVVFVSRNY
Sbjct: 302  HIGEKEGIILEAETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNY 361

Query: 635  FSFDKRRRLFRVGSLKWLSGLPPNHISQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVH 456
            FS DKRRRLFRVGSLKW+S  PP  I+QL+CKVRHGPGFYNCS TME G+D HEDVAVV 
Sbjct: 362  FSLDKRRRLFRVGSLKWISREPPGQINQLQCKVRHGPGFYNCSFTMEPGEDGHEDVAVVQ 421

Query: 455  LSGDDQGLAAGQFAAFYQGKTCLGSGIILESWDDQGFPVCAKALEIARMEDKSLLGKPVK 276
            LS DDQGLAAGQF AFYQG++C+GSG+ILESWDDQGFPVCAKALEIARMEDKS LGKPVK
Sbjct: 422  LSEDDQGLAAGQFTAFYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPVK 481

Query: 275  IKVKPEKTHFMEANQKGVIELNRGQSSRSASAAIKQRSQASQGEVISRFPMKLLQKFRNK 96
            IKV+PE T    ++Q   I+L+R + + S +A   + S++S+GE  SRFPM  LQK R K
Sbjct: 482  IKVRPE-TPPKGSDQIDGIKLDR-ELANSQAAVTDEISRSSEGEARSRFPMNWLQKIREK 539


>ref|XP_009376883.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like isoform
            X1 [Pyrus x bretschneideri]
          Length = 579

 Score =  779 bits (2011), Expect = 0.0
 Identities = 385/500 (77%), Positives = 424/500 (84%), Gaps = 4/500 (0%)
 Frame = -2

Query: 1637 SIPLKPHV-PFFHN---RTSLFLRPLXXXXXXXXXXAELLTPNSIESFNISLQPYLSCCM 1470
            ++PLKP++ PF  +    T LF+RP           +   TP S  SFN  + PYLSC M
Sbjct: 82   TLPLKPYLWPFPRSPTPTTRLFVRPFSLSARSVNCFSVPQTPRSSNSFNGDVGPYLSCSM 141

Query: 1469 PHKRFRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYA 1290
            PH+R +VAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYA
Sbjct: 142  PHQRLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYA 201

Query: 1289 KAVCSQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTRIKFGAFMDAISKM 1110
            KAVC+QVDVPLEVVHLTDEYW+NVVSYII+EY+CGRTPNPDVLCNTRIKFGAFMD+I+ M
Sbjct: 202  KAVCNQVDVPLEVVHLTDEYWKNVVSYIIEEYQCGRTPNPDVLCNTRIKFGAFMDSINSM 261

Query: 1109 DFDYVASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQAQLKRLIFPLGCVS 930
             FDYVASGHYANV+H   SQTD  SVLELS DMVKDQTYFL++LSQAQL+RLIFPLGC+S
Sbjct: 262  KFDYVASGHYANVVHPPASQTDKDSVLELSQDMVKDQTYFLSNLSQAQLRRLIFPLGCLS 321

Query: 929  KEEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGIILEAETGDLLGKHR 750
            K+EVRKLATKFDLPNKDRKDSQGICFLGKIKF+EF+AR IGEKEG+ILEAE+GD LG HR
Sbjct: 322  KDEVRKLATKFDLPNKDRKDSQGICFLGKIKFSEFIARHIGEKEGVILEAESGDFLGNHR 381

Query: 749  GFWFYTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRLFRVGSLKWLSGLP 570
            GFWFYTIGQRQGLRLPGGPWYVV KD++NNVVFVSRNYFS DKRRRLFRVGSLKWL+GL 
Sbjct: 382  GFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSVDKRRRLFRVGSLKWLNGLS 441

Query: 569  PNHISQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLAAGQFAAFYQGKTC 390
            PN ISQL CKVRHGP F  CS  ME G+D HEDVAVVHLS DDQGLAAGQFAAFYQG+TC
Sbjct: 442  PNQISQLECKVRHGPAFCKCSFIMEPGEDGHEDVAVVHLSQDDQGLAAGQFAAFYQGRTC 501

Query: 389  LGSGIILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKPEKTHFMEANQKGVIELN 210
            LGSG+ILESWDDQGFPVCA+A EIARMEDKS+LGKPVKIKVKPE     E+ +   +EL 
Sbjct: 502  LGSGVILESWDDQGFPVCARAHEIARMEDKSILGKPVKIKVKPENL-LRESGEVDGVELC 560

Query: 209  RGQSSRSASAAIKQRSQASQ 150
             G  +    AA KQ  Q SQ
Sbjct: 561  GGLKN-PGIAATKQPMQVSQ 579


>ref|XP_009347315.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like [Pyrus
            x bretschneideri] gi|694441086|ref|XP_009347316.1|
            PREDICTED: mitochondrial tRNA-specific 2-thiouridylase
            1-like [Pyrus x bretschneideri]
          Length = 588

 Score =  775 bits (2000), Expect = 0.0
 Identities = 380/490 (77%), Positives = 419/490 (85%), Gaps = 4/490 (0%)
 Frame = -2

Query: 1637 SIPLKPHV---PFFHNRTS-LFLRPLXXXXXXXXXXAELLTPNSIESFNISLQPYLSCCM 1470
            ++PLKP++   P F   T+ LFLRP           +   TP S  SFN  + PYLSC M
Sbjct: 21   TLPLKPYLWPFPRFPTPTTRLFLRPFSLSARSVNCFSVPQTPRSSNSFNGDVGPYLSCSM 80

Query: 1469 PHKRFRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYA 1290
            PH+R +VAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYA
Sbjct: 81   PHQRLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYA 140

Query: 1289 KAVCSQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTRIKFGAFMDAISKM 1110
            KAVC+QVDVPLEVVHLTDEYW+NVVSYII+EY+CGRTPNPDVLCNTRIKFGAFMD+I+ M
Sbjct: 141  KAVCNQVDVPLEVVHLTDEYWKNVVSYIIEEYQCGRTPNPDVLCNTRIKFGAFMDSINSM 200

Query: 1109 DFDYVASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQAQLKRLIFPLGCVS 930
             FDYVASGHYANV+H   SQTD  SVLELS DMVKDQTYFL++LSQAQL+RLIFPLGC+S
Sbjct: 201  KFDYVASGHYANVVHPPASQTDKDSVLELSQDMVKDQTYFLSNLSQAQLRRLIFPLGCLS 260

Query: 929  KEEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGIILEAETGDLLGKHR 750
            K+EVRKLATKFDLPNKDRKDSQGICFLGKIKF+EF+AR IGEKEG+ILEAE+GD LG HR
Sbjct: 261  KDEVRKLATKFDLPNKDRKDSQGICFLGKIKFSEFIARHIGEKEGVILEAESGDFLGNHR 320

Query: 749  GFWFYTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRLFRVGSLKWLSGLP 570
            GFWFYTIGQRQGLRLPGGPWYVV KD++NNVVFVSRNYFS DKRRRLFRVGSLKWL+GL 
Sbjct: 321  GFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSVDKRRRLFRVGSLKWLNGLS 380

Query: 569  PNHISQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLAAGQFAAFYQGKTC 390
            PN ISQL CKVRHGP F  CS  ME G+D HEDVAVVHLS DDQGLAAGQFAAFYQG+TC
Sbjct: 381  PNQISQLECKVRHGPAFCKCSFIMEPGEDGHEDVAVVHLSQDDQGLAAGQFAAFYQGRTC 440

Query: 389  LGSGIILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKPEKTHFMEANQKGVIELN 210
            LGSG+ILESWDDQGFPVCA+A EIARMEDKSLLGKPVKIKV+PE     E+ +   +EL 
Sbjct: 441  LGSGVILESWDDQGFPVCARAHEIARMEDKSLLGKPVKIKVEPENL-LRESGEVDGVELC 499

Query: 209  RGQSSRSASA 180
             G  +   +A
Sbjct: 500  GGLKNPGIAA 509


>ref|XP_009376884.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like isoform
            X2 [Pyrus x bretschneideri]
          Length = 578

 Score =  773 bits (1995), Expect = 0.0
 Identities = 384/500 (76%), Positives = 423/500 (84%), Gaps = 4/500 (0%)
 Frame = -2

Query: 1637 SIPLKPHV-PFFHN---RTSLFLRPLXXXXXXXXXXAELLTPNSIESFNISLQPYLSCCM 1470
            ++PLKP++ PF  +    T LF+RP           +   TP S  SFN  + PYLSC M
Sbjct: 82   TLPLKPYLWPFPRSPTPTTRLFVRPFSLSARSVNCFSVPQTPRSSNSFNGDVGPYLSCSM 141

Query: 1469 PHKRFRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYA 1290
            PH+R +VAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYA
Sbjct: 142  PHQRLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYA 201

Query: 1289 KAVCSQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTRIKFGAFMDAISKM 1110
            KAVC+QVDVPLEVVHLTDEYW+NV SYII+EY+CGRTPNPDVLCNTRIKFGAFMD+I+ M
Sbjct: 202  KAVCNQVDVPLEVVHLTDEYWKNV-SYIIEEYQCGRTPNPDVLCNTRIKFGAFMDSINSM 260

Query: 1109 DFDYVASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQAQLKRLIFPLGCVS 930
             FDYVASGHYANV+H   SQTD  SVLELS DMVKDQTYFL++LSQAQL+RLIFPLGC+S
Sbjct: 261  KFDYVASGHYANVVHPPASQTDKDSVLELSQDMVKDQTYFLSNLSQAQLRRLIFPLGCLS 320

Query: 929  KEEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGIILEAETGDLLGKHR 750
            K+EVRKLATKFDLPNKDRKDSQGICFLGKIKF+EF+AR IGEKEG+ILEAE+GD LG HR
Sbjct: 321  KDEVRKLATKFDLPNKDRKDSQGICFLGKIKFSEFIARHIGEKEGVILEAESGDFLGNHR 380

Query: 749  GFWFYTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRLFRVGSLKWLSGLP 570
            GFWFYTIGQRQGLRLPGGPWYVV KD++NNVVFVSRNYFS DKRRRLFRVGSLKWL+GL 
Sbjct: 381  GFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSVDKRRRLFRVGSLKWLNGLS 440

Query: 569  PNHISQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLAAGQFAAFYQGKTC 390
            PN ISQL CKVRHGP F  CS  ME G+D HEDVAVVHLS DDQGLAAGQFAAFYQG+TC
Sbjct: 441  PNQISQLECKVRHGPAFCKCSFIMEPGEDGHEDVAVVHLSQDDQGLAAGQFAAFYQGRTC 500

Query: 389  LGSGIILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKPEKTHFMEANQKGVIELN 210
            LGSG+ILESWDDQGFPVCA+A EIARMEDKS+LGKPVKIKVKPE     E+ +   +EL 
Sbjct: 501  LGSGVILESWDDQGFPVCARAHEIARMEDKSILGKPVKIKVKPENL-LRESGEVDGVELC 559

Query: 209  RGQSSRSASAAIKQRSQASQ 150
             G  +    AA KQ  Q SQ
Sbjct: 560  GGLKN-PGIAATKQPMQVSQ 578


>ref|XP_008385559.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like isoform
            X1 [Malus domestica]
          Length = 549

 Score =  766 bits (1979), Expect = 0.0
 Identities = 383/501 (76%), Positives = 417/501 (83%), Gaps = 6/501 (1%)
 Frame = -2

Query: 1637 SIPLKPHVPFFHNRTSLFLRPLXXXXXXXXXXAELL-----TPNSIESFNISLQPYLSCC 1473
            S+PLKP++      + LFLRP           +        TP    S N  + PYLSC 
Sbjct: 21   SLPLKPYLSL---TSLLFLRPFSRSCADCSPRSVHCFAVPQTPRPPNSLNGDVGPYLSCS 77

Query: 1472 MPHKRFRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKY 1293
            MPH+  +VAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKY
Sbjct: 78   MPHQSLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKY 137

Query: 1292 AKAVCSQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTRIKFGAFMDAISK 1113
            A AVC+QV VPLEVVHLTDEYW+NVVSYII  Y+CGRTPNPDVLCNTRIKFGAFMDAIS 
Sbjct: 138  ANAVCNQVXVPLEVVHLTDEYWKNVVSYIIXXYQCGRTPNPDVLCNTRIKFGAFMDAISS 197

Query: 1112 MDFDYVASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQAQLKRLIFPLGCV 933
            M FD+VASGHYANV+H   SQTD  SVLELS DMVKDQTYFL+HLSQAQLKRLIFPLGC+
Sbjct: 198  MKFDHVASGHYANVVHPPASQTDKDSVLELSQDMVKDQTYFLSHLSQAQLKRLIFPLGCL 257

Query: 932  SKEEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGIILEAETGDLLGKH 753
            SK+EVRKLATKFDLPNKDRKDSQGICFLGKIKFNEF+AR IGEKEG+ILEAE+GD LG H
Sbjct: 258  SKDEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFIARHIGEKEGVILEAESGDFLGNH 317

Query: 752  RGFWFYTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRLFRVGSLKWLSGL 573
            RGFWFYTIGQRQGLRLPGGPWYVV KD++NNVVFVSRNYFS  KRRRLFRVGSLKWL+GL
Sbjct: 318  RGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSDKKRRRLFRVGSLKWLNGL 377

Query: 572  PPNHISQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLAAGQFAAFYQGKT 393
            PPN ISQL CKVRHGP F NCSL ME G+D HEDVAVVHLS DDQGLAAGQFAAFYQG T
Sbjct: 378  PPNQISQLECKVRHGPAFCNCSLIMEPGEDGHEDVAVVHLSQDDQGLAAGQFAAFYQGXT 437

Query: 392  CLGSGIILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKPEKTHFMEANQKGVIEL 213
            CLGSG+ILESWDDQGFPVCA+A+EIAR+EDKSLLGKPVKIKVKPE     E+ +    EL
Sbjct: 438  CLGSGVILESWDDQGFPVCARAIEIARLEDKSLLGKPVKIKVKPENV-ITESGEADGTEL 496

Query: 212  NRG-QSSRSASAAIKQRSQAS 153
              G ++SR A+A      QA+
Sbjct: 497  CGGLENSRIAAAKPPTLMQAA 517


>ref|XP_008385560.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like isoform
            X2 [Malus domestica]
          Length = 522

 Score =  766 bits (1978), Expect = 0.0
 Identities = 381/492 (77%), Positives = 414/492 (84%), Gaps = 6/492 (1%)
 Frame = -2

Query: 1637 SIPLKPHVPFFHNRTSLFLRPLXXXXXXXXXXAELL-----TPNSIESFNISLQPYLSCC 1473
            S+PLKP++      + LFLRP           +        TP    S N  + PYLSC 
Sbjct: 21   SLPLKPYLSL---TSLLFLRPFSRSCADCSPRSVHCFAVPQTPRPPNSLNGDVGPYLSCS 77

Query: 1472 MPHKRFRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKY 1293
            MPH+  +VAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKY
Sbjct: 78   MPHQSLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKY 137

Query: 1292 AKAVCSQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTRIKFGAFMDAISK 1113
            A AVC+QV VPLEVVHLTDEYW+NVVSYII  Y+CGRTPNPDVLCNTRIKFGAFMDAIS 
Sbjct: 138  ANAVCNQVXVPLEVVHLTDEYWKNVVSYIIXXYQCGRTPNPDVLCNTRIKFGAFMDAISS 197

Query: 1112 MDFDYVASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQAQLKRLIFPLGCV 933
            M FD+VASGHYANV+H   SQTD  SVLELS DMVKDQTYFL+HLSQAQLKRLIFPLGC+
Sbjct: 198  MKFDHVASGHYANVVHPPASQTDKDSVLELSQDMVKDQTYFLSHLSQAQLKRLIFPLGCL 257

Query: 932  SKEEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGIILEAETGDLLGKH 753
            SK+EVRKLATKFDLPNKDRKDSQGICFLGKIKFNEF+AR IGEKEG+ILEAE+GD LG H
Sbjct: 258  SKDEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFIARHIGEKEGVILEAESGDFLGNH 317

Query: 752  RGFWFYTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRLFRVGSLKWLSGL 573
            RGFWFYTIGQRQGLRLPGGPWYVV KD++NNVVFVSRNYFS  KRRRLFRVGSLKWL+GL
Sbjct: 318  RGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSDKKRRRLFRVGSLKWLNGL 377

Query: 572  PPNHISQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLAAGQFAAFYQGKT 393
            PPN ISQL CKVRHGP F NCSL ME G+D HEDVAVVHLS DDQGLAAGQFAAFYQG T
Sbjct: 378  PPNQISQLECKVRHGPAFCNCSLIMEPGEDGHEDVAVVHLSQDDQGLAAGQFAAFYQGXT 437

Query: 392  CLGSGIILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKPEKTHFMEANQKGVIEL 213
            CLGSG+ILESWDDQGFPVCA+A+EIAR+EDKSLLGKPVKIKVKPE     E+ +    EL
Sbjct: 438  CLGSGVILESWDDQGFPVCARAIEIARLEDKSLLGKPVKIKVKPENV-ITESGEADGTEL 496

Query: 212  NRG-QSSRSASA 180
              G ++SR A+A
Sbjct: 497  CGGLENSRIAAA 508


>ref|XP_008344848.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like [Malus
            domestica]
          Length = 581

 Score =  762 bits (1968), Expect = 0.0
 Identities = 374/490 (76%), Positives = 413/490 (84%), Gaps = 4/490 (0%)
 Frame = -2

Query: 1637 SIPLKPHV-PFFHNRTS---LFLRPLXXXXXXXXXXAELLTPNSIESFNISLQPYLSCCM 1470
            ++ LKP++ PF  + T    LFLRP           +   TP +  SFN  + PYLSC M
Sbjct: 88   TLALKPYIWPFPRSPTPTTLLFLRPFSLSARSVNCFSVPQTPRAPNSFNGDVGPYLSCSM 147

Query: 1469 PHKRFRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYA 1290
            PH+  +VAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFEN+WSECPWEEDLKYA
Sbjct: 148  PHQSLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENYWSECPWEEDLKYA 207

Query: 1289 KAVCSQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTRIKFGAFMDAISKM 1110
            KA C+QVDVPLEVVHLTDEYW+NVVSYII+EY+CGRTPNPD+LCNTRIKFGAFMDAIS M
Sbjct: 208  KAXCNQVDVPLEVVHLTDEYWKNVVSYIIEEYQCGRTPNPDILCNTRIKFGAFMDAISSM 267

Query: 1109 DFDYVASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQAQLKRLIFPLGCVS 930
             FDYVASGHYANV+H   SQTD  SVLELS DMVKDQTYFL++LSQAQL+RLIFPLGC+S
Sbjct: 268  KFDYVASGHYANVVHPPASQTDKDSVLELSQDMVKDQTYFLSNLSQAQLRRLIFPLGCLS 327

Query: 929  KEEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGIILEAETGDLLGKHR 750
            K+EVRKLATKFDLPNKDRKDSQGICFLGKIKF+EF+AR IGEKEG+ILEAE+GD LG HR
Sbjct: 328  KDEVRKLATKFDLPNKDRKDSQGICFLGKIKFSEFIARHIGEKEGVILEAESGDFLGNHR 387

Query: 749  GFWFYTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRLFRVGSLKWLSGLP 570
            GFWFYTIGQRQGL LPGGPWYVV KD++NNVVFVSRNYFS DKRRRLFR GSLKWL+GL 
Sbjct: 388  GFWFYTIGQRQGLHLPGGPWYVVEKDIKNNVVFVSRNYFSVDKRRRLFRXGSLKWLNGLS 447

Query: 569  PNHISQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLAAGQFAAFYQGKTC 390
            PN ISQL CKVRHGP F  CS  ME G+D HEDVAVVHLS DDQGLAAGQFAAFYQG+TC
Sbjct: 448  PNQISQLECKVRHGPAFCKCSFIMEXGEDGHEDVAVVHLSQDDQGLAAGQFAAFYQGRTC 507

Query: 389  LGSGIILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKPEKTHFMEANQKGVIELN 210
            LGSG+ILESWDDQGFPVCA+A E ARMEDKSLLGKPVKIKVKPE     E+ +   +EL 
Sbjct: 508  LGSGVILESWDDQGFPVCARAHENARMEDKSLLGKPVKIKVKPESL-LTESGEVDGVELC 566

Query: 209  RGQSSRSASA 180
             G  +   +A
Sbjct: 567  GGLKNPGIAA 576


>ref|XP_009375052.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 isoform X2
            [Pyrus x bretschneideri]
          Length = 525

 Score =  761 bits (1965), Expect = 0.0
 Identities = 378/496 (76%), Positives = 419/496 (84%), Gaps = 1/496 (0%)
 Frame = -2

Query: 1637 SIPLKPHVPFFHNRTSLFLRPLXXXXXXXXXXAELLTPNSIESFNISLQPYLSCCMPHKR 1458
            S+PLKP++      + LFL+P           +    PNS+   N  + PYLSC MPH+ 
Sbjct: 21   SLPLKPYLSL---TSLLFLKPFSRSCADSSPSSR--PPNSL---NGDVGPYLSCSMPHQS 72

Query: 1457 FRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYAKAVC 1278
             +VAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYA AVC
Sbjct: 73   LKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYANAVC 132

Query: 1277 SQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTRIKFGAFMDAISKMDFDY 1098
            +QVDVPLEVVHLTDEYW+NVVSYII+EY+CGRTPNPDVLCNTRIKFG+FMDAIS M FD 
Sbjct: 133  NQVDVPLEVVHLTDEYWKNVVSYIIEEYQCGRTPNPDVLCNTRIKFGSFMDAISSMKFDR 192

Query: 1097 VASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQAQLKRLIFPLGCVSKEEV 918
            VASGHYANV+H   S+TD  SVLELS DMVKDQTYFL+HLSQAQLKRLIFPLGC+SK+EV
Sbjct: 193  VASGHYANVVHPPASETDKDSVLELSQDMVKDQTYFLSHLSQAQLKRLIFPLGCLSKDEV 252

Query: 917  RKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGIILEAETGDLLGKHRGFWF 738
            RKLATKFDLPNKDRKDSQGICFLGKIKFNEF+AR IGE +G+ILEAE+GD LG HRGFWF
Sbjct: 253  RKLATKFDLPNKDRKDSQGICFLGKIKFNEFIARHIGENKGVILEAESGDFLGNHRGFWF 312

Query: 737  YTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRLFRVGSLKWLSGLPPNHI 558
            YTIGQRQGLRLPGGPWYVV KD++NNVVFVSRNYFS +KRR LFRVGSLKWL+GLPPN I
Sbjct: 313  YTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSDEKRRHLFRVGSLKWLNGLPPNQI 372

Query: 557  SQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLAAGQFAAFYQGKTCLGSG 378
            SQL CKVRHGP F NCSL ME G+D HEDVAVVHLS DDQGLAAGQFAAFY+G+TCLGSG
Sbjct: 373  SQLECKVRHGPAFCNCSLIMEPGEDGHEDVAVVHLSQDDQGLAAGQFAAFYRGRTCLGSG 432

Query: 377  IILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKPEKTHFMEANQKGVIELNRG-Q 201
            +ILESWDDQGFP+CA ALEIAR+EDKSLLGKPVKIKVKPE     E+ +    EL  G +
Sbjct: 433  VILESWDDQGFPMCAIALEIARLEDKSLLGKPVKIKVKPENV-ITESGEADGTELCGGLE 491

Query: 200  SSRSASAAIKQRSQAS 153
            +SR A+A      QA+
Sbjct: 492  NSRIAAAKPPTPMQAA 507


>ref|XP_009375053.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 isoform X3
            [Pyrus x bretschneideri]
          Length = 512

 Score =  761 bits (1964), Expect = 0.0
 Identities = 376/487 (77%), Positives = 416/487 (85%), Gaps = 1/487 (0%)
 Frame = -2

Query: 1637 SIPLKPHVPFFHNRTSLFLRPLXXXXXXXXXXAELLTPNSIESFNISLQPYLSCCMPHKR 1458
            S+PLKP++      + LFL+P           +    PNS+   N  + PYLSC MPH+ 
Sbjct: 21   SLPLKPYLSL---TSLLFLKPFSRSCADSSPSSR--PPNSL---NGDVGPYLSCSMPHQS 72

Query: 1457 FRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYAKAVC 1278
             +VAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYA AVC
Sbjct: 73   LKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYANAVC 132

Query: 1277 SQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTRIKFGAFMDAISKMDFDY 1098
            +QVDVPLEVVHLTDEYW+NVVSYII+EY+CGRTPNPDVLCNTRIKFG+FMDAIS M FD 
Sbjct: 133  NQVDVPLEVVHLTDEYWKNVVSYIIEEYQCGRTPNPDVLCNTRIKFGSFMDAISSMKFDR 192

Query: 1097 VASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQAQLKRLIFPLGCVSKEEV 918
            VASGHYANV+H   S+TD  SVLELS DMVKDQTYFL+HLSQAQLKRLIFPLGC+SK+EV
Sbjct: 193  VASGHYANVVHPPASETDKDSVLELSQDMVKDQTYFLSHLSQAQLKRLIFPLGCLSKDEV 252

Query: 917  RKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGIILEAETGDLLGKHRGFWF 738
            RKLATKFDLPNKDRKDSQGICFLGKIKFNEF+AR IGE +G+ILEAE+GD LG HRGFWF
Sbjct: 253  RKLATKFDLPNKDRKDSQGICFLGKIKFNEFIARHIGENKGVILEAESGDFLGNHRGFWF 312

Query: 737  YTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRLFRVGSLKWLSGLPPNHI 558
            YTIGQRQGLRLPGGPWYVV KD++NNVVFVSRNYFS +KRR LFRVGSLKWL+GLPPN I
Sbjct: 313  YTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSDEKRRHLFRVGSLKWLNGLPPNQI 372

Query: 557  SQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLAAGQFAAFYQGKTCLGSG 378
            SQL CKVRHGP F NCSL ME G+D HEDVAVVHLS DDQGLAAGQFAAFY+G+TCLGSG
Sbjct: 373  SQLECKVRHGPAFCNCSLIMEPGEDGHEDVAVVHLSQDDQGLAAGQFAAFYRGRTCLGSG 432

Query: 377  IILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKPEKTHFMEANQKGVIELNRG-Q 201
            +ILESWDDQGFP+CA ALEIAR+EDKSLLGKPVKIKVKPE     E+ +    EL  G +
Sbjct: 433  VILESWDDQGFPMCAIALEIARLEDKSLLGKPVKIKVKPENV-ITESGEADGTELCGGLE 491

Query: 200  SSRSASA 180
            +SR A+A
Sbjct: 492  NSRIAAA 498


>ref|XP_009375051.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 isoform X1
            [Pyrus x bretschneideri]
          Length = 527

 Score =  761 bits (1964), Expect = 0.0
 Identities = 376/487 (77%), Positives = 416/487 (85%), Gaps = 1/487 (0%)
 Frame = -2

Query: 1637 SIPLKPHVPFFHNRTSLFLRPLXXXXXXXXXXAELLTPNSIESFNISLQPYLSCCMPHKR 1458
            S+PLKP++      + LFL+P           +    PNS+   N  + PYLSC MPH+ 
Sbjct: 21   SLPLKPYLSL---TSLLFLKPFSRSCADSSPSSR--PPNSL---NGDVGPYLSCSMPHQS 72

Query: 1457 FRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYAKAVC 1278
             +VAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYA AVC
Sbjct: 73   LKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWEEDLKYANAVC 132

Query: 1277 SQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTRIKFGAFMDAISKMDFDY 1098
            +QVDVPLEVVHLTDEYW+NVVSYII+EY+CGRTPNPDVLCNTRIKFG+FMDAIS M FD 
Sbjct: 133  NQVDVPLEVVHLTDEYWKNVVSYIIEEYQCGRTPNPDVLCNTRIKFGSFMDAISSMKFDR 192

Query: 1097 VASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQAQLKRLIFPLGCVSKEEV 918
            VASGHYANV+H   S+TD  SVLELS DMVKDQTYFL+HLSQAQLKRLIFPLGC+SK+EV
Sbjct: 193  VASGHYANVVHPPASETDKDSVLELSQDMVKDQTYFLSHLSQAQLKRLIFPLGCLSKDEV 252

Query: 917  RKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGIILEAETGDLLGKHRGFWF 738
            RKLATKFDLPNKDRKDSQGICFLGKIKFNEF+AR IGE +G+ILEAE+GD LG HRGFWF
Sbjct: 253  RKLATKFDLPNKDRKDSQGICFLGKIKFNEFIARHIGENKGVILEAESGDFLGNHRGFWF 312

Query: 737  YTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRLFRVGSLKWLSGLPPNHI 558
            YTIGQRQGLRLPGGPWYVV KD++NNVVFVSRNYFS +KRR LFRVGSLKWL+GLPPN I
Sbjct: 313  YTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSDEKRRHLFRVGSLKWLNGLPPNQI 372

Query: 557  SQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLAAGQFAAFYQGKTCLGSG 378
            SQL CKVRHGP F NCSL ME G+D HEDVAVVHLS DDQGLAAGQFAAFY+G+TCLGSG
Sbjct: 373  SQLECKVRHGPAFCNCSLIMEPGEDGHEDVAVVHLSQDDQGLAAGQFAAFYRGRTCLGSG 432

Query: 377  IILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKPEKTHFMEANQKGVIELNRG-Q 201
            +ILESWDDQGFP+CA ALEIAR+EDKSLLGKPVKIKVKPE     E+ +    EL  G +
Sbjct: 433  VILESWDDQGFPMCAIALEIARLEDKSLLGKPVKIKVKPENV-ITESGEADGTELCGGLE 491

Query: 200  SSRSASA 180
            +SR A+A
Sbjct: 492  NSRIAAA 498


>ref|XP_006470603.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like [Citrus
            sinensis]
          Length = 525

 Score =  758 bits (1956), Expect = 0.0
 Identities = 376/481 (78%), Positives = 414/481 (86%), Gaps = 6/481 (1%)
 Frame = -2

Query: 1520 SIESFNISLQP-YLSCCMPHKR-FRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQ 1347
            S+ +    ++P YLSC MPH++  +VAVL+SGGVDSSVALRLLH+AGHSCTAFYLKIWFQ
Sbjct: 47   SVSTAATQIEPRYLSCSMPHQKGLKVAVLLSGGVDSSVALRLLHSAGHSCTAFYLKIWFQ 106

Query: 1346 EDFENFWSECPWEEDLKYAKAVCSQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPD 1167
            EDFENFW+ECPWEED+KYAKAVC QVDVPLEVVHLTDEYW+NVVSYII+EYRCGRTPNPD
Sbjct: 107  EDFENFWTECPWEEDMKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPD 166

Query: 1166 VLCNTRIKFGAFMDAISKMDFDYVASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFL 987
            VLCNTRIKFGAFMDAIS M+FDYVASGHYA V+HSS  Q    SVLELS D VKDQTYFL
Sbjct: 167  VLCNTRIKFGAFMDAISSMEFDYVASGHYAKVVHSSADQEYKPSVLELSKDKVKDQTYFL 226

Query: 986  AHLSQAQLKRLIFPLGCVSKEEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIG 807
            +HLSQAQLKRLIFPLGC+SKEEVRK AT+FDLPNKDRKDSQGICFLGKIKF+EFVAR IG
Sbjct: 227  SHLSQAQLKRLIFPLGCISKEEVRKFATQFDLPNKDRKDSQGICFLGKIKFSEFVARHIG 286

Query: 806  EKEGIILEAETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSF 627
            E EG+ILEAETGD LGKH GFWFYTIGQRQGLRLPGGPWYVV KDV+NNVVFVSRNY+SF
Sbjct: 287  EMEGVILEAETGDFLGKHWGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSF 346

Query: 626  DKRRRLFRVGSLKWLSGLPPNHISQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSG 447
            DK+RRLFRVGSLKWLSGLPP  IS+L+CKVRHGPGFYNC+L +E  +D  E++AVV LS 
Sbjct: 347  DKKRRLFRVGSLKWLSGLPPEEISRLQCKVRHGPGFYNCNLMIEHVEDGGENIAVVQLSE 406

Query: 446  DDQGLAAGQFAAFYQGKTCLGSGIILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKV 267
            DDQGLAAGQFAAFYQG  C+GSG+ILESWDDQGFPVC KALEIARMEDKS LGKP+KIKV
Sbjct: 407  DDQGLAAGQFAAFYQGTRCIGSGVILESWDDQGFPVCEKALEIARMEDKSKLGKPIKIKV 466

Query: 266  KPEKTHFMEANQKGVIELNRGQSSR---SASAAIKQRSQ-ASQGEVISRFPMKLLQKFRN 99
            KPE      +N KGV      QSSR   +A  +I ++S   SQ E ISRFPM  LQK R 
Sbjct: 467  KPETPEEFNSN-KGV------QSSRNLVNAQNSIAEKSNTTSQEEAISRFPMNWLQKLRE 519

Query: 98   K 96
            K
Sbjct: 520  K 520


>gb|KDO61266.1| hypothetical protein CISIN_1g042717mg, partial [Citrus sinensis]
          Length = 474

 Score =  757 bits (1954), Expect = 0.0
 Identities = 374/469 (79%), Positives = 408/469 (86%), Gaps = 5/469 (1%)
 Frame = -2

Query: 1487 YLSCCMPHKR-FRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW 1311
            YLSC MPH++  +VAVL+SGGVDSSVALRLLH+AGHSCTAFYLKIWFQEDFENFW+ECPW
Sbjct: 8    YLSCSMPHQKGLKVAVLLSGGVDSSVALRLLHSAGHSCTAFYLKIWFQEDFENFWTECPW 67

Query: 1310 EEDLKYAKAVCSQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTRIKFGAF 1131
            EED+KYAKAVC QVDVPLEVVHLTDEYW+NVVSYII+EYRCGRTPNPDVLCNTRIKFGAF
Sbjct: 68   EEDMKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF 127

Query: 1130 MDAISKMDFDYVASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQAQLKRLI 951
            MDAIS M+FDYVASGHYA V+HSS  Q    SVLELS D VKDQTYFL+HLSQAQLKRLI
Sbjct: 128  MDAISSMEFDYVASGHYAKVVHSSADQEYKPSVLELSKDKVKDQTYFLSHLSQAQLKRLI 187

Query: 950  FPLGCVSKEEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGIILEAETG 771
            FPLGC+SKEEVRK AT+FDLPNKDRKDSQGICFLGKIKF+EFVAR IGE EG+ILEAETG
Sbjct: 188  FPLGCISKEEVRKFATQFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGVILEAETG 247

Query: 770  DLLGKHRGFWFYTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRLFRVGSL 591
            D LGKH GFWFYTIGQRQGLRLPGGPWYVV KDV+NNVVFVSRNY+SFDK+RRLFRVGSL
Sbjct: 248  DFLGKHWGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSFDKKRRLFRVGSL 307

Query: 590  KWLSGLPPNHISQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLAAGQFAA 411
            KWLSGLPP  IS+L+CKVRHGPGFYNC+L +E  +D  E++AVV LS DDQGLAAGQFAA
Sbjct: 308  KWLSGLPPEEISRLQCKVRHGPGFYNCNLMIEHVEDGGENIAVVQLSEDDQGLAAGQFAA 367

Query: 410  FYQGKTCLGSGIILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKPEKTHFMEANQ 231
            FYQG  C+GSG+ILESWDDQGFPVC KALEIARMEDKS LGKP+KIKVKPE      +N 
Sbjct: 368  FYQGTRCIGSGVILESWDDQGFPVCEKALEIARMEDKSKLGKPIKIKVKPETPEEFNSN- 426

Query: 230  KGVIELNRGQSSR---SASAAIKQRSQ-ASQGEVISRFPMKLLQKFRNK 96
            KGV      QSSR   +A  +I ++S   SQ E ISRFPM  LQK R K
Sbjct: 427  KGV------QSSRNLVNAQNSIAEKSNTTSQEEAISRFPMNWLQKLREK 469


>ref|XP_008449720.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Cucumis
            melo]
          Length = 539

 Score =  746 bits (1926), Expect = 0.0
 Identities = 355/465 (76%), Positives = 403/465 (86%)
 Frame = -2

Query: 1490 PYLSCCMPHKRFRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW 1311
            P+LSC MP    +VAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW
Sbjct: 72   PFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW 131

Query: 1310 EEDLKYAKAVCSQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTRIKFGAF 1131
            ++DLKYAKAVC Q+DVPLEVVHLT+EYW NVVSYII+EYRCGRTPNPDVLCNTRIKFGAF
Sbjct: 132  DDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF 191

Query: 1130 MDAISKMDFDYVASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQAQLKRLI 951
            MDAIS M+FDYVASGHYANVIH S  Q D  SVLELS DMVKDQTYFL+HLSQ QLKRL+
Sbjct: 192  MDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLL 251

Query: 950  FPLGCVSKEEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGIILEAETG 771
            FPLGC+ K+EVRKLA KF+LPNKDRKDSQGICFLGKIKF+EFVAR IGE EG+ILEAE+G
Sbjct: 252  FPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESG 311

Query: 770  DLLGKHRGFWFYTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRLFRVGSL 591
            D LGKHRGFWFYT+GQRQGLRLPGGPWYVV KD++NNVVFVSRNYFSFDKRRR+FRVGSL
Sbjct: 312  DFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSL 371

Query: 590  KWLSGLPPNHISQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLAAGQFAA 411
            KWLSG+PP+  + L+CKVRHGPGFY+C+LTME  KD HEDVAVVHLS DDQGLAAGQFAA
Sbjct: 372  KWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAA 431

Query: 410  FYQGKTCLGSGIILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKPEKTHFMEANQ 231
            FY+G++C+GSG+ILESWDDQGFPVC KA+E A+MEDKS LGKP+KIKVKP+ +   E+  
Sbjct: 432  FYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPD-SGMNESKA 490

Query: 230  KGVIELNRGQSSRSASAAIKQRSQASQGEVISRFPMKLLQKFRNK 96
            K   ++ + +S    +  ++Q  + S  E ++  PMK L   R K
Sbjct: 491  KDDTKI-QDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKK 534


>ref|XP_012446852.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Gossypium
            raimondii] gi|763788378|gb|KJB55374.1| hypothetical
            protein B456_009G073000 [Gossypium raimondii]
          Length = 490

 Score =  742 bits (1915), Expect = 0.0
 Identities = 356/471 (75%), Positives = 403/471 (85%), Gaps = 2/471 (0%)
 Frame = -2

Query: 1667 RVVGVMAMPSSIPLKPHVPFFHNRTSLFLRPLXXXXXXXXXXAELLTPNSIESFN-ISLQ 1491
            R+  ++++PSS  L  H P   + +S    P             +   N+  +FN + L 
Sbjct: 16   RLTPIVSLPSSHFLHYHFPKPTSSSSTASLPQTLPTSYSLPPKPVSPLNTAATFNNVILD 75

Query: 1490 P-YLSCCMPHKRFRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECP 1314
            P +LSCCMP KR +VAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFEN+W+ECP
Sbjct: 76   PRHLSCCMPDKRLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENYWTECP 135

Query: 1313 WEEDLKYAKAVCSQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTRIKFGA 1134
            WEEDLKYA+AVC QVDVPL+VVHLTDEYW+NVVSYII+EYRCGRTPNPDVLCNTRIKFGA
Sbjct: 136  WEEDLKYARAVCDQVDVPLQVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGA 195

Query: 1133 FMDAISKMDFDYVASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQAQLKRL 954
            FMDAI  MD+DY+ASGHYANV+H S  Q++  S+LELS DMVKDQTYFL+HLSQ QLK+L
Sbjct: 196  FMDAIYNMDYDYIASGHYANVVHPSADQSNKASILELSQDMVKDQTYFLSHLSQFQLKQL 255

Query: 953  IFPLGCVSKEEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGIILEAET 774
            IFPLGC+SK+EVR+LA KFDLPNK RKDSQGICFLGKI+F++FVAR IGE EGIILEAET
Sbjct: 256  IFPLGCLSKDEVRQLAAKFDLPNKGRKDSQGICFLGKIRFSDFVARHIGEMEGIILEAET 315

Query: 773  GDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRLFRVGS 594
            GD LGKHRGFWFYTIGQRQGLRLPGGPWYVV KDV+NNVVFVSRNYFS DKRRR+FRVGS
Sbjct: 316  GDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVQKDVKNNVVFVSRNYFSLDKRRRVFRVGS 375

Query: 593  LKWLSGLPPNHISQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLAAGQFA 414
            LKW SG PPNHI QL+CKVRHGPGFYNC+  ME G+D ++D+AVV LS DDQGLAAGQFA
Sbjct: 376  LKWFSGSPPNHIDQLQCKVRHGPGFYNCNFKMEHGEDGNDDIAVVQLSEDDQGLAAGQFA 435

Query: 413  AFYQGKTCLGSGIILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKP 261
            AFY+G+TC+GSG+ILESWDD+GFPVCAKALEIARMEDKS LGKPVKI  KP
Sbjct: 436  AFYEGQTCIGSGVILESWDDRGFPVCAKALEIARMEDKSELGKPVKIMTKP 486


>ref|XP_004142116.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Cucumis
            sativus] gi|700199017|gb|KGN54175.1| hypothetical protein
            Csa_4G291390 [Cucumis sativus]
          Length = 538

 Score =  741 bits (1912), Expect = 0.0
 Identities = 355/465 (76%), Positives = 401/465 (86%)
 Frame = -2

Query: 1490 PYLSCCMPHKRFRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW 1311
            P+LSC MP    +VAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW
Sbjct: 71   PFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW 130

Query: 1310 EEDLKYAKAVCSQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTRIKFGAF 1131
            ++DLKYAKAVC QVDVPLEVVHLT+EYW NVVSYII+EYRCGRTPNPDVLCNTRIKFGAF
Sbjct: 131  DDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF 190

Query: 1130 MDAISKMDFDYVASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQAQLKRLI 951
            MDAIS M+FDYVASGHYANVIH S  Q D  SVLELS DMVKDQTYFL+HLSQ QLKRL+
Sbjct: 191  MDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLL 250

Query: 950  FPLGCVSKEEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGIILEAETG 771
            FPLGC+ K+EVRKLA KF+LPNKDRKDSQGICFLGKIKF+EFVAR IGE EG+ILEAE+G
Sbjct: 251  FPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESG 310

Query: 770  DLLGKHRGFWFYTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRLFRVGSL 591
            D LGKHRGFWFYTIGQRQGLRLPGGPWYVV KD++NNVVFVSRNYFSFDKRRR+FRVGSL
Sbjct: 311  DFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSL 370

Query: 590  KWLSGLPPNHISQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLAAGQFAA 411
            KWLSG PP+  ++L+CKVRHGP FY+C+LTME  +D HEDVAVVHLS DDQGLAAGQFAA
Sbjct: 371  KWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQGLAAGQFAA 430

Query: 410  FYQGKTCLGSGIILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKPEKTHFMEANQ 231
            FY+G++C+GSG+ILESWDDQGFPVC KA+E A+MEDKS LGKP+KIKVKPE +   E+  
Sbjct: 431  FYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPE-SGMKESKA 489

Query: 230  KGVIELNRGQSSRSASAAIKQRSQASQGEVISRFPMKLLQKFRNK 96
            K   E++    +  A+  ++Q  + S  E ++   MK L   + K
Sbjct: 490  KDDTEIHDKLRNEEAT-VVEQIREVSPDEAMATPSMKWLGSLKKK 533


>ref|XP_010257320.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like
            [Nelumbo nucifera]
          Length = 531

 Score =  737 bits (1903), Expect = 0.0
 Identities = 358/470 (76%), Positives = 397/470 (84%)
 Frame = -2

Query: 1505 NISLQPYLSCCMPHKRFRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFW 1326
            N  L+PYLSC MP KR +VAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFW
Sbjct: 63   NTVLEPYLSCSMPGKRLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFW 122

Query: 1325 SECPWEEDLKYAKAVCSQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTRI 1146
            S CPWEEDL+Y KAVC QV+VPLEVVHLTDEYW+NVVSYII EYR GRTPNPDVLCNTRI
Sbjct: 123  SACPWEEDLEYVKAVCDQVEVPLEVVHLTDEYWDNVVSYIIQEYRYGRTPNPDVLCNTRI 182

Query: 1145 KFGAFMDAISKMDFDYVASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQAQ 966
            KFGAF+DAIS M+FDYVASGHYANVIH    Q D  S+LELS D VKDQTYFL+HLSQAQ
Sbjct: 183  KFGAFLDAISNMEFDYVASGHYANVIHPCTDQKDGPSILELSEDKVKDQTYFLSHLSQAQ 242

Query: 965  LKRLIFPLGCVSKEEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGIIL 786
            LKRL+FPLGC+SK EVRKLA K +LPN+DRKDSQGICFLGKIKF+EF+A+ IGE EGI+L
Sbjct: 243  LKRLLFPLGCISKNEVRKLAAKLELPNRDRKDSQGICFLGKIKFSEFIAKHIGEMEGILL 302

Query: 785  EAETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRLF 606
            EAETGD LGKH GFWFYTIGQRQGLRLPGGPWYVV KDV+NNVVFVSRNY+SFDKRRR F
Sbjct: 303  EAETGDFLGKHHGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSFDKRRRRF 362

Query: 605  RVGSLKWLSGLPPNHISQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLAA 426
            RVGSLKWL+GLPP+  ++L+CKVRHGPGFY+CSL ME+G+D     AVVHLS DDQGLAA
Sbjct: 363  RVGSLKWLAGLPPDQFNKLQCKVRHGPGFYDCSLIMETGEDGDRTSAVVHLSEDDQGLAA 422

Query: 425  GQFAAFYQGKTCLGSGIILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKPEKTHF 246
            GQFAAFYQGKTC+GSG+ILE WD QGFPVCAKAL+IARMEDKS LGKPVKIKV PE +  
Sbjct: 423  GQFAAFYQGKTCIGSGVILECWDSQGFPVCAKALQIARMEDKSKLGKPVKIKVNPENS-L 481

Query: 245  MEANQKGVIELNRGQSSRSASAAIKQRSQASQGEVISRFPMKLLQKFRNK 96
              +     +ELN G+S  S    + +  Q+SQG     F +   QK R K
Sbjct: 482  KSSEITDKVELN-GKSLNSEDITLDKSKQSSQG----IFNVNWFQKLREK 526


>ref|XP_012089993.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Jatropha
            curcas] gi|643705955|gb|KDP22087.1| hypothetical protein
            JCGZ_25918 [Jatropha curcas]
          Length = 505

 Score =  736 bits (1900), Expect = 0.0
 Identities = 353/435 (81%), Positives = 389/435 (89%), Gaps = 1/435 (0%)
 Frame = -2

Query: 1505 NISLQPY-LSCCMPHKRFRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENF 1329
            N S++PY LSCCMP KR RVAVL+SGGVDSSVALRLLHAAGH CTAFYLKIWFQEDFENF
Sbjct: 63   NGSIEPYYLSCCMPDKRLRVAVLLSGGVDSSVALRLLHAAGHQCTAFYLKIWFQEDFENF 122

Query: 1328 WSECPWEEDLKYAKAVCSQVDVPLEVVHLTDEYWENVVSYIIDEYRCGRTPNPDVLCNTR 1149
            WSECPWEEDL+YAKAVC QVDVPLEVVHLTDEYW+NVVSYII+EYRCGRTPNPD+LCNTR
Sbjct: 123  WSECPWEEDLEYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDILCNTR 182

Query: 1148 IKFGAFMDAISKMDFDYVASGHYANVIHSSRSQTDNISVLELSNDMVKDQTYFLAHLSQA 969
            IKFGAFMDAI+ M+FDYVASGHYANVIH S  + +  SVLELS DMVKDQTYFL+HLSQA
Sbjct: 183  IKFGAFMDAINSMEFDYVASGHYANVIHPSTDKINKSSVLELSKDMVKDQTYFLSHLSQA 242

Query: 968  QLKRLIFPLGCVSKEEVRKLATKFDLPNKDRKDSQGICFLGKIKFNEFVARQIGEKEGII 789
            QLKRLIFPLG + K+EVRK+A +FDLPNKDRKDSQGICFLGKIKF+EFVAR IGEKEGII
Sbjct: 243  QLKRLIFPLGGIPKDEVRKIAKQFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEKEGII 302

Query: 788  LEAETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVGKDVRNNVVFVSRNYFSFDKRRRL 609
            LEAETGD LG HRGFWFYTIGQRQGLRLPGGPWYVV KD +NNVVFVSRNYFSFDKRR L
Sbjct: 303  LEAETGDFLGTHRGFWFYTIGQRQGLRLPGGPWYVVAKDTKNNVVFVSRNYFSFDKRRCL 362

Query: 608  FRVGSLKWLSGLPPNHISQLRCKVRHGPGFYNCSLTMESGKDSHEDVAVVHLSGDDQGLA 429
            FRVGSL+W SG PP   S L+CKVRHGPGFY+CS+ +  G+   EDVAVV L+ DDQGLA
Sbjct: 363  FRVGSLRWFSGSPPTKTSVLQCKVRHGPGFYDCSVVINPGEVESEDVAVVRLTEDDQGLA 422

Query: 428  AGQFAAFYQGKTCLGSGIILESWDDQGFPVCAKALEIARMEDKSLLGKPVKIKVKPEKTH 249
            AGQFAAFYQG+TC+GSG+ILESWDDQGFP+C +ALE+ARMEDKS LGKPVKIKVKPE  H
Sbjct: 423  AGQFAAFYQGRTCIGSGVILESWDDQGFPICERALELARMEDKSKLGKPVKIKVKPEIPH 482

Query: 248  FMEANQKGVIELNRG 204
              E++++  I L+RG
Sbjct: 483  --ESDKRDDIGLDRG 495