BLASTX nr result
ID: Zingiber23_contig00033097
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Zingiber23_contig00033097
(305 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAD17487.1| berberine bridge enzyme [Berberis stolonifera] 110 2e-22
ref|NP_001061035.1| Os08g0158200 [Oryza sativa Japonica Group] g... 108 1e-21
gb|EAZ05661.1| hypothetical protein OsI_27888 [Oryza sativa Indi... 108 1e-21
gb|EEC83421.1| hypothetical protein OsI_28887 [Oryza sativa Indi... 107 1e-21
sp|P93479.1|RETO_PAPSO RecName: Full=Reticuline oxidase; AltName... 107 2e-21
dbj|BAC65012.1| putative berberine bridge enzyme [Oryza sativa J... 107 2e-21
ref|XP_006659851.1| PREDICTED: reticuline oxidase-like [Oryza br... 106 3e-21
gb|EXC32491.1| Reticuline oxidase [Morus notabilis] 105 5e-21
ref|XP_006846477.1| hypothetical protein AMTR_s00018p00124850 [A... 105 5e-21
gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana] 105 6e-21
gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana] 105 8e-21
gb|ACO90218.1| berberine bridge enzyme [Eschscholzia californica] 105 8e-21
ref|XP_002299045.1| hypothetical protein POPTR_0001s46910g [Popu... 105 8e-21
ref|XP_002333677.1| predicted protein [Populus trichocarpa] 105 8e-21
sp|P30986.1|RETO_ESCCA RecName: Full=Reticuline oxidase; AltName... 104 1e-20
dbj|BAM44344.1| berberine bridge enzyme [Coptis japonica] 104 1e-20
pdb|4EC3|A Chain A, Structure Of Berberine Bridge Enzyme, H174a ... 104 1e-20
pdb|3GSY|A Chain A, Structure Of Berberine Bridge Enzyme In Comp... 104 1e-20
pdb|3FWA|A Chain A, Structure Of Berberine Bridge Enzyme, C166a ... 104 1e-20
pdb|3FW9|A Chain A, Structure Of Berberine Bridge Enzyme In Comp... 104 1e-20
>gb|AAD17487.1| berberine bridge enzyme [Berberis stolonifera]
Length = 536
Score = 110 bits (275), Expect = 2e-22
Identities = 53/82 (64%), Positives = 63/82 (76%)
Frame = -1
Query: 251 LRSAVLCSREAGYSVRLRSGGHSYEGLSYSASAPFVVVDLMRLNRVIVDSASRTAWVESG 72
L ++V+CS +++RLRSGGHSYEGLSY A PFVV+DLM LNR+ +D S+TAWVESG
Sbjct: 79 LAASVVCSNRGLWTIRLRSGGHSYEGLSYVADTPFVVIDLMNLNRISIDLESKTAWVESG 138
Query: 71 ATLGETYHGISAASDDLAFSAG 6
ATLGE Y IS ASD L FS G
Sbjct: 139 ATLGEIYCAISEASDTLGFSGG 160
>ref|NP_001061035.1| Os08g0158200 [Oryza sativa Japonica Group]
gi|28812072|dbj|BAC65010.1| putative Reticuline oxidase
precursor [Oryza sativa Japonica Group]
gi|113623004|dbj|BAF22949.1| Os08g0158200 [Oryza sativa
Japonica Group] gi|125602251|gb|EAZ41576.1| hypothetical
protein OsJ_26110 [Oryza sativa Japonica Group]
gi|215697462|dbj|BAG91456.1| unnamed protein product
[Oryza sativa Japonica Group]
Length = 529
Score = 108 bits (269), Expect = 1e-21
Identities = 52/85 (61%), Positives = 64/85 (75%), Gaps = 2/85 (2%)
Frame = -1
Query: 251 LRSAVLCSREAGYSVRLRSGGHSYEGLSYSAS--APFVVVDLMRLNRVIVDSASRTAWVE 78
LR A+LC+R + ++R+RSGGHSYEGLSY+ PFVV+DLM LNRV VDS S TAW E
Sbjct: 86 LRRAILCARNSSLAIRVRSGGHSYEGLSYTTENHVPFVVIDLMNLNRVQVDSVSATAWAE 145
Query: 77 SGATLGETYHGISAASDDLAFSAGS 3
+GATLGE Y+ + +S LAFS GS
Sbjct: 146 AGATLGELYYAVGRSSQSLAFSGGS 170
>gb|EAZ05661.1| hypothetical protein OsI_27888 [Oryza sativa Indica Group]
Length = 562
Score = 108 bits (269), Expect = 1e-21
Identities = 60/90 (66%), Positives = 70/90 (77%), Gaps = 3/90 (3%)
Frame = -1
Query: 263 TQLHLRSAVLCSREAGY-SVRLRSGGHSYEGLSYSAS--APFVVVDLMRLNRVIVDSASR 93
T+ LR AV C+REAG +VRLRSGGHSYEG+SYS FVVVDL+ L+ V VD+ASR
Sbjct: 81 TRGELRDAVRCAREAGLGAVRLRSGGHSYEGVSYSGEDDGGFVVVDLLALDGVRVDAASR 140
Query: 92 TAWVESGATLGETYHGISAASDDLAFSAGS 3
TAWVESGATLG+ Y ++AAS LAFSAGS
Sbjct: 141 TAWVESGATLGQVYQAVAAASPALAFSAGS 170
>gb|EEC83421.1| hypothetical protein OsI_28887 [Oryza sativa Indica Group]
Length = 424
Score = 107 bits (268), Expect = 1e-21
Identities = 55/91 (60%), Positives = 67/91 (73%), Gaps = 8/91 (8%)
Frame = -1
Query: 251 LRSAVLCSREAGYSVRLRSGGHSYEGLSYSAS--------APFVVVDLMRLNRVIVDSAS 96
L SAVLC+R A +R+RSGGHSYEGLSY+ FVV+DLMR+NRV VD+AS
Sbjct: 100 LTSAVLCARHASLRIRVRSGGHSYEGLSYTVGDGGDDADRVRFVVIDLMRMNRVRVDAAS 159
Query: 95 RTAWVESGATLGETYHGISAASDDLAFSAGS 3
TAWVESGATLGE Y+ ++++S LAF AGS
Sbjct: 160 ATAWVESGATLGEIYYAVASSSSSLAFPAGS 190
>sp|P93479.1|RETO_PAPSO RecName: Full=Reticuline oxidase; AltName: Full=Berberine
bridge-forming enzyme; Short=BBE; AltName:
Full=Tetrahydroprotoberberine synthase; Flags: Precursor
gi|3282517|gb|AAC61839.1| berberine bridge enzyme
[Papaver somniferum]
Length = 535
Score = 107 bits (266), Expect = 2e-21
Identities = 49/82 (59%), Positives = 62/82 (75%)
Frame = -1
Query: 251 LRSAVLCSREAGYSVRLRSGGHSYEGLSYSASAPFVVVDLMRLNRVIVDSASRTAWVESG 72
L S V C +++RLRSGGHSYEGLSY+A PFV+VD+M LNR+ +D S TAWVESG
Sbjct: 87 LSSTVHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLNRISIDVLSETAWVESG 146
Query: 71 ATLGETYHGISAASDDLAFSAG 6
ATLGE Y+ I+ ++D L F+AG
Sbjct: 147 ATLGELYYAIAQSTDTLGFTAG 168
>dbj|BAC65012.1| putative berberine bridge enzyme [Oryza sativa Japonica Group]
Length = 562
Score = 107 bits (266), Expect = 2e-21
Identities = 59/90 (65%), Positives = 70/90 (77%), Gaps = 3/90 (3%)
Frame = -1
Query: 263 TQLHLRSAVLCSREAGY-SVRLRSGGHSYEGLSYSAS--APFVVVDLMRLNRVIVDSASR 93
T+ LR AV C+REAG +VRLRSGGHSYEG+SY+ FVVVDL+ L+ V VD+ASR
Sbjct: 81 TRGELRDAVRCAREAGLGAVRLRSGGHSYEGVSYTGEDDGGFVVVDLLALDGVRVDAASR 140
Query: 92 TAWVESGATLGETYHGISAASDDLAFSAGS 3
TAWVESGATLG+ Y ++AAS LAFSAGS
Sbjct: 141 TAWVESGATLGQVYQAVAAASPALAFSAGS 170
>ref|XP_006659851.1| PREDICTED: reticuline oxidase-like [Oryza brachyantha]
Length = 527
Score = 106 bits (265), Expect = 3e-21
Identities = 53/89 (59%), Positives = 67/89 (75%), Gaps = 2/89 (2%)
Frame = -1
Query: 263 TQLHLRSAVLCSREAGYSVRLRSGGHSYEGLSYSAS--APFVVVDLMRLNRVIVDSASRT 90
+++ L+ A+LC+R++ ++R+RSGGHSYEGLSY+ FVVVDLM LNRV VD S T
Sbjct: 80 SKVDLQRAILCARDSSLAIRVRSGGHSYEGLSYTTENHVSFVVVDLMNLNRVQVDMVSNT 139
Query: 89 AWVESGATLGETYHGISAASDDLAFSAGS 3
AWVESGATLGE YH I ++ LAF AGS
Sbjct: 140 AWVESGATLGELYHAIGQSNMSLAFPAGS 168
>gb|EXC32491.1| Reticuline oxidase [Morus notabilis]
Length = 557
Score = 105 bits (263), Expect = 5e-21
Identities = 50/81 (61%), Positives = 65/81 (80%)
Frame = -1
Query: 245 SAVLCSREAGYSVRLRSGGHSYEGLSYSASAPFVVVDLMRLNRVIVDSASRTAWVESGAT 66
SAVLCSR+A Y + +RSGGHSYEG S A+ PFV++D+M L ++ +D S+TAWVE+GAT
Sbjct: 102 SAVLCSRKASYKIIVRSGGHSYEGTSSIATPPFVIIDMMNLKQITLDLESKTAWVEAGAT 161
Query: 65 LGETYHGISAASDDLAFSAGS 3
LGETY+ I+ AS+ AFSAGS
Sbjct: 162 LGETYYAIAKASNVHAFSAGS 182
>ref|XP_006846477.1| hypothetical protein AMTR_s00018p00124850 [Amborella trichopoda]
gi|548849287|gb|ERN08152.1| hypothetical protein
AMTR_s00018p00124850 [Amborella trichopoda]
Length = 526
Score = 105 bits (263), Expect = 5e-21
Identities = 47/87 (54%), Positives = 63/87 (72%)
Frame = -1
Query: 263 TQLHLRSAVLCSREAGYSVRLRSGGHSYEGLSYSASAPFVVVDLMRLNRVIVDSASRTAW 84
T L S +LCS+E + +R+R GGHSYEGLS + F+++DLM LN + VD ++TAW
Sbjct: 76 THAQLSSTILCSKETSHEIRIRCGGHSYEGLSSTGDRSFIIIDLMNLNNIHVDLETQTAW 135
Query: 83 VESGATLGETYHGISAASDDLAFSAGS 3
VE+GATLGETY+ I+ A+ D FSAGS
Sbjct: 136 VEAGATLGETYYSIARATSDFGFSAGS 162
>gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana]
Length = 554
Score = 105 bits (262), Expect = 6e-21
Identities = 48/82 (58%), Positives = 61/82 (74%)
Frame = -1
Query: 251 LRSAVLCSREAGYSVRLRSGGHSYEGLSYSASAPFVVVDLMRLNRVIVDSASRTAWVESG 72
L + V C +++RLRSGGHSYEGLSY+A PFV++DLM LNR+ +D S TAWVESG
Sbjct: 95 LSNTVRCCTRGSWTIRLRSGGHSYEGLSYTADTPFVLIDLMNLNRISIDIDSETAWVESG 154
Query: 71 ATLGETYHGISAASDDLAFSAG 6
ATLGE Y+ I+ +D L F+AG
Sbjct: 155 ATLGELYYAITELTDSLGFTAG 176
>gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana]
Length = 536
Score = 105 bits (261), Expect = 8e-21
Identities = 47/82 (57%), Positives = 61/82 (74%)
Frame = -1
Query: 251 LRSAVLCSREAGYSVRLRSGGHSYEGLSYSASAPFVVVDLMRLNRVIVDSASRTAWVESG 72
L + V C +++RLRSGGHSYEGLSY+A PFV++DLM LNR+ +D S TAWVESG
Sbjct: 90 LSNTVRCCTRGSWTIRLRSGGHSYEGLSYTADTPFVLIDLMNLNRISIDMDSETAWVESG 149
Query: 71 ATLGETYHGISAASDDLAFSAG 6
AT+GE Y+ IS ++D F+AG
Sbjct: 150 ATVGELYYAISESTDSFGFTAG 171
>gb|ACO90218.1| berberine bridge enzyme [Eschscholzia californica]
Length = 242
Score = 105 bits (261), Expect = 8e-21
Identities = 47/82 (57%), Positives = 63/82 (76%)
Frame = -1
Query: 251 LRSAVLCSREAGYSVRLRSGGHSYEGLSYSASAPFVVVDLMRLNRVIVDSASRTAWVESG 72
L + + C R+ +++RLRSGGHSYEGLSY++ PF++VDLM LNRV +D S TAWVESG
Sbjct: 83 LSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILVDLMNLNRVSIDLESETAWVESG 142
Query: 71 ATLGETYHGISAASDDLAFSAG 6
+TLGE Y+ I+ +S L F+AG
Sbjct: 143 STLGELYYAITESSSKLGFTAG 164
>ref|XP_002299045.1| hypothetical protein POPTR_0001s46910g [Populus trichocarpa]
gi|222846303|gb|EEE83850.1| hypothetical protein
POPTR_0001s46910g [Populus trichocarpa]
Length = 533
Score = 105 bits (261), Expect = 8e-21
Identities = 48/84 (57%), Positives = 64/84 (76%)
Frame = -1
Query: 254 HLRSAVLCSREAGYSVRLRSGGHSYEGLSYSASAPFVVVDLMRLNRVIVDSASRTAWVES 75
H+++A+LCS+ +R+RSGGH +EGLSY A+ PFV++DL+ L V VD+ SRTAWV++
Sbjct: 88 HIQAAILCSQRHNLQIRIRSGGHDFEGLSYMAALPFVIIDLISLRAVNVDATSRTAWVQA 147
Query: 74 GATLGETYHGISAASDDLAFSAGS 3
GATLGE Y+ IS S LAF AGS
Sbjct: 148 GATLGELYYSISEKSRTLAFPAGS 171
>ref|XP_002333677.1| predicted protein [Populus trichocarpa]
Length = 228
Score = 105 bits (261), Expect = 8e-21
Identities = 48/84 (57%), Positives = 64/84 (76%)
Frame = -1
Query: 254 HLRSAVLCSREAGYSVRLRSGGHSYEGLSYSASAPFVVVDLMRLNRVIVDSASRTAWVES 75
H+++A+LCS+ +R+RSGGH +EGLSY A+ PFV++DL+ L V VD+ SRTAWV++
Sbjct: 88 HIQAAILCSQRHNLQIRIRSGGHDFEGLSYMAALPFVIIDLISLRAVNVDATSRTAWVQA 147
Query: 74 GATLGETYHGISAASDDLAFSAGS 3
GATLGE Y+ IS S LAF AGS
Sbjct: 148 GATLGELYYSISEKSRTLAFPAGS 171
>sp|P30986.1|RETO_ESCCA RecName: Full=Reticuline oxidase; AltName: Full=Berberine
bridge-forming enzyme; Short=BBE; AltName:
Full=Tetrahydroprotoberberine synthase; Flags: Precursor
gi|239110|gb|AAB20352.1| (S)-reticuline:oxygen
oxidoreductase (methylene-bridge-forming) [Eschscholzia
californica] gi|2897944|gb|AAC39358.1| berberine bridge
enzyme [Eschscholzia californica]
Length = 538
Score = 104 bits (260), Expect = 1e-20
Identities = 46/82 (56%), Positives = 63/82 (76%)
Frame = -1
Query: 251 LRSAVLCSREAGYSVRLRSGGHSYEGLSYSASAPFVVVDLMRLNRVIVDSASRTAWVESG 72
L + + C R+ +++RLRSGGHSYEGLSY++ PF+++DLM LNRV +D S TAWVESG
Sbjct: 83 LSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESG 142
Query: 71 ATLGETYHGISAASDDLAFSAG 6
+TLGE Y+ I+ +S L F+AG
Sbjct: 143 STLGELYYAITESSSKLGFTAG 164
>dbj|BAM44344.1| berberine bridge enzyme [Coptis japonica]
Length = 533
Score = 104 bits (260), Expect = 1e-20
Identities = 47/82 (57%), Positives = 62/82 (75%)
Frame = -1
Query: 251 LRSAVLCSREAGYSVRLRSGGHSYEGLSYSASAPFVVVDLMRLNRVIVDSASRTAWVESG 72
L + + C R +++RLRSGGHSYEGLS++A +PFV++D+M LN V +D ++TAW ESG
Sbjct: 83 LVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVIIDMMNLNGVSIDLDTQTAWAESG 142
Query: 71 ATLGETYHGISAASDDLAFSAG 6
ATLGE YH I +SD L FSAG
Sbjct: 143 ATLGEIYHAIGVSSDVLGFSAG 164
>pdb|4EC3|A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In
Complex With (S)-Reticuline
Length = 519
Score = 104 bits (260), Expect = 1e-20
Identities = 46/82 (56%), Positives = 63/82 (76%)
Frame = -1
Query: 251 LRSAVLCSREAGYSVRLRSGGHSYEGLSYSASAPFVVVDLMRLNRVIVDSASRTAWVESG 72
L + + C R+ +++RLRSGGHSYEGLSY++ PF+++DLM LNRV +D S TAWVESG
Sbjct: 64 LSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESG 123
Query: 71 ATLGETYHGISAASDDLAFSAG 6
+TLGE Y+ I+ +S L F+AG
Sbjct: 124 STLGELYYAITESSSKLGFTAG 145
>pdb|3GSY|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
Dehydroscoulerine
Length = 519
Score = 104 bits (260), Expect = 1e-20
Identities = 46/82 (56%), Positives = 63/82 (76%)
Frame = -1
Query: 251 LRSAVLCSREAGYSVRLRSGGHSYEGLSYSASAPFVVVDLMRLNRVIVDSASRTAWVESG 72
L + + C R+ +++RLRSGGHSYEGLSY++ PF+++DLM LNRV +D S TAWVESG
Sbjct: 64 LSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESG 123
Query: 71 ATLGETYHGISAASDDLAFSAG 6
+TLGE Y+ I+ +S L F+AG
Sbjct: 124 STLGELYYAITESSSKLGFTAG 145
>pdb|3FWA|A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant In
Complex With (S)-Reticuline
Length = 497
Score = 104 bits (260), Expect = 1e-20
Identities = 46/82 (56%), Positives = 63/82 (76%)
Frame = -1
Query: 251 LRSAVLCSREAGYSVRLRSGGHSYEGLSYSASAPFVVVDLMRLNRVIVDSASRTAWVESG 72
L + + C R+ +++RLRSGGHSYEGLSY++ PF+++DLM LNRV +D S TAWVESG
Sbjct: 58 LSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESG 117
Query: 71 ATLGETYHGISAASDDLAFSAG 6
+TLGE Y+ I+ +S L F+AG
Sbjct: 118 STLGELYYAITESSSKLGFTAG 139
>pdb|3FW9|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
(S)-Scoulerine
Length = 495
Score = 104 bits (260), Expect = 1e-20
Identities = 46/82 (56%), Positives = 63/82 (76%)
Frame = -1
Query: 251 LRSAVLCSREAGYSVRLRSGGHSYEGLSYSASAPFVVVDLMRLNRVIVDSASRTAWVESG 72
L + + C R+ +++RLRSGGHSYEGLSY++ PF+++DLM LNRV +D S TAWVESG
Sbjct: 58 LSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESG 117
Query: 71 ATLGETYHGISAASDDLAFSAG 6
+TLGE Y+ I+ +S L F+AG
Sbjct: 118 STLGELYYAITESSSKLGFTAG 139