BLASTX nr result
ID: Zingiber23_contig00027213
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Zingiber23_contig00027213
(1373 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI16383.3| unnamed protein product [Vitis vinifera] 576 e-162
ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnm... 576 e-162
gb|EXB93298.1| tRNA-specific 2-thiouridylase mnmA [Morus notabilis] 568 e-159
ref|XP_003558694.1| PREDICTED: tRNA-specific 2-thiouridylase mnm... 564 e-158
gb|EEC74610.1| hypothetical protein OsI_10221 [Oryza sativa Indi... 562 e-157
ref|XP_004507093.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm... 561 e-157
ref|XP_006649485.1| PREDICTED: mitochondrial tRNA-specific 2-thi... 560 e-157
ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm... 560 e-157
ref|NP_001049129.1| Os03g0174600 [Oryza sativa Japonica Group] g... 559 e-156
ref|XP_002468413.1| hypothetical protein SORBIDRAFT_01g045520 [S... 557 e-156
ref|XP_003539065.2| PREDICTED: mitochondrial tRNA-specific 2-thi... 556 e-155
ref|XP_006470603.1| PREDICTED: mitochondrial tRNA-specific 2-thi... 556 e-155
gb|ESW04011.1| hypothetical protein PHAVU_011G059600g [Phaseolus... 556 e-155
ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m... 555 e-155
gb|EMT11416.1| tRNA-specific 2-thiouridylase mnmA [Aegilops taus... 553 e-155
ref|XP_004985520.1| PREDICTED: mitochondrial tRNA-specific 2-thi... 547 e-153
ref|XP_006358437.1| PREDICTED: mitochondrial tRNA-specific 2-thi... 546 e-153
ref|XP_006850208.1| hypothetical protein AMTR_s00022p00251260 [A... 546 e-152
ref|NP_001131207.1| uncharacterized protein LOC100192515 [Zea ma... 544 e-152
dbj|BAK01046.1| predicted protein [Hordeum vulgare subsp. vulgare] 544 e-152
>emb|CBI16383.3| unnamed protein product [Vitis vinifera]
Length = 544
Score = 576 bits (1485), Expect = e-162
Identities = 278/366 (75%), Positives = 305/366 (83%), Gaps = 3/366 (0%)
Frame = +2
Query: 278 ADLEPYLGCSMPGKRLKVAVLLSGGVDSSVALRLLHAAGHDITAFYLKIWFQEDFENFWS 457
ADL+PYL CS+P RLK+AVLLSGGVDSSVALRLLHAAGH TAFYLKIWFQEDFENFWS
Sbjct: 73 ADLQPYLSCSLPNNRLKIAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 132
Query: 458 ECPWDEDLKYATIVCDQVDVQFEVVHLTDEYWKNVVSHIINEYRSGRTPNPDVLCNTRVK 637
ECPW++DLKYA VCDQVDV EVVHLTDEYWKNVVS+II EYR GRTPNPDVLCNTR+K
Sbjct: 133 ECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIK 192
Query: 638 FGAFLEAIGSRGFDYVASGHYAHVIHS--SSKDGLSILKLSKDMVKDQTYFXXXXXXXXX 811
FGAF++AI S+ FDYVASGHYA+V+HS D S+L+LSKDMVKDQTYF
Sbjct: 193 FGAFMDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVKDQTYFLSHLSQIQL 252
Query: 812 XXXXFPLGCITKEEVRSIASLMDLPNKDRKDSQGICFLGKVKFSEFVAKHIGEMEGIMLE 991
FPLGCI K+EVRS+A+ +DLPN+DRKDSQGICFLGK+KFSEFVA+HIGE EGI+LE
Sbjct: 253 KRLIFPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKEGIILE 312
Query: 992 AETGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVLFVSRNYFSLNKRRLTFR 1171
AETGD LGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDV+NNV+FVSRNYFSL+KRR FR
Sbjct: 313 AETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRLFR 372
Query: 1172 VESLNWFSGSSPINVEQLQCKVRHGPGFYECSITSGSGISDEESL-VVHLSEDDQGLAAG 1348
V SL W S P + QLQCKVRHGPGFY CS T G E + VV LSEDDQGLAAG
Sbjct: 373 VGSLKWISREPPGQINQLQCKVRHGPGFYNCSFTMEPGEDGHEDVAVVQLSEDDQGLAAG 432
Query: 1349 QFAAFY 1366
QF AFY
Sbjct: 433 QFTAFY 438
>ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnmA-like [Vitis vinifera]
Length = 540
Score = 576 bits (1485), Expect = e-162
Identities = 278/366 (75%), Positives = 305/366 (83%), Gaps = 3/366 (0%)
Frame = +2
Query: 278 ADLEPYLGCSMPGKRLKVAVLLSGGVDSSVALRLLHAAGHDITAFYLKIWFQEDFENFWS 457
ADL+PYL CS+P RLK+AVLLSGGVDSSVALRLLHAAGH TAFYLKIWFQEDFENFWS
Sbjct: 69 ADLQPYLSCSLPNNRLKIAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 128
Query: 458 ECPWDEDLKYATIVCDQVDVQFEVVHLTDEYWKNVVSHIINEYRSGRTPNPDVLCNTRVK 637
ECPW++DLKYA VCDQVDV EVVHLTDEYWKNVVS+II EYR GRTPNPDVLCNTR+K
Sbjct: 129 ECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIK 188
Query: 638 FGAFLEAIGSRGFDYVASGHYAHVIHS--SSKDGLSILKLSKDMVKDQTYFXXXXXXXXX 811
FGAF++AI S+ FDYVASGHYA+V+HS D S+L+LSKDMVKDQTYF
Sbjct: 189 FGAFMDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVKDQTYFLSHLSQIQL 248
Query: 812 XXXXFPLGCITKEEVRSIASLMDLPNKDRKDSQGICFLGKVKFSEFVAKHIGEMEGIMLE 991
FPLGCI K+EVRS+A+ +DLPN+DRKDSQGICFLGK+KFSEFVA+HIGE EGI+LE
Sbjct: 249 KRLIFPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKEGIILE 308
Query: 992 AETGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVLFVSRNYFSLNKRRLTFR 1171
AETGD LGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDV+NNV+FVSRNYFSL+KRR FR
Sbjct: 309 AETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRLFR 368
Query: 1172 VESLNWFSGSSPINVEQLQCKVRHGPGFYECSITSGSGISDEESL-VVHLSEDDQGLAAG 1348
V SL W S P + QLQCKVRHGPGFY CS T G E + VV LSEDDQGLAAG
Sbjct: 369 VGSLKWISREPPGQINQLQCKVRHGPGFYNCSFTMEPGEDGHEDVAVVQLSEDDQGLAAG 428
Query: 1349 QFAAFY 1366
QF AFY
Sbjct: 429 QFTAFY 434
>gb|EXB93298.1| tRNA-specific 2-thiouridylase mnmA [Morus notabilis]
Length = 580
Score = 568 bits (1464), Expect = e-159
Identities = 274/365 (75%), Positives = 303/365 (83%), Gaps = 3/365 (0%)
Frame = +2
Query: 281 DLEPYLGCSMPGKRLKVAVLLSGGVDSSVALRLLHAAGHDITAFYLKIWFQEDFENFWSE 460
D+EPYL CSMP KRL+VAVLLSGGVDSSVALRLLHAAGH TAFYLKIWFQEDFENFWSE
Sbjct: 116 DIEPYLSCSMPHKRLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSE 175
Query: 461 CPWDEDLKYATIVCDQVDVQFEVVHLTDEYWKNVVSHIINEYRSGRTPNPDVLCNTRVKF 640
CPW+EDLKYA VC Q++V EVVHLTDEYWKNVVS+II+EYR GRTPNPDVLCNTR+KF
Sbjct: 176 CPWEEDLKYAKAVCSQIEVPLEVVHLTDEYWKNVVSYIIDEYRCGRTPNPDVLCNTRIKF 235
Query: 641 GAFLEAIGSRGFDYVASGHYAHVIHS--SSKDGLSILKLSKDMVKDQTYFXXXXXXXXXX 814
G+F++AI S FDYVASGHYA+V+HS + + S+L+LS+DMVKDQTYF
Sbjct: 236 GSFMDAISSMEFDYVASGHYANVVHSFTNERGDASVLELSQDMVKDQTYFLSHLSQAQLK 295
Query: 815 XXXFPLGCITKEEVRSIASLMDLPNKDRKDSQGICFLGKVKFSEFVAKHIGEMEGIMLEA 994
FPLGCI+KEEVR +A DLPNKDRKDSQGICFLGK+KFSEFVA+HIGE EGI+LEA
Sbjct: 296 RLVFPLGCISKEEVRKLAKKFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEKEGIILEA 355
Query: 995 ETGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVLFVSRNYFSLNKRRLTFRV 1174
ETGD+LG HRGFWFYTIGQRQGLRLPGGPWYVVEKDV+NNV+F SRNYFS +KRR FRV
Sbjct: 356 ETGDFLGMHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFASRNYFSFDKRRRIFRV 415
Query: 1175 ESLNWFSGSSPINVEQLQCKVRHGPGFYECSITSGSG-ISDEESLVVHLSEDDQGLAAGQ 1351
SL W +G P QLQCKVRHGPGFY CS T SG S E+ VV LSEDDQGLA GQ
Sbjct: 416 GSLKWLNGLPPDQTCQLQCKVRHGPGFYNCSFTMESGENSGEDVAVVQLSEDDQGLAVGQ 475
Query: 1352 FAAFY 1366
FAAFY
Sbjct: 476 FAAFY 480
>ref|XP_003558694.1| PREDICTED: tRNA-specific 2-thiouridylase mnmA-like [Brachypodium
distachyon]
Length = 481
Score = 564 bits (1453), Expect = e-158
Identities = 281/399 (70%), Positives = 314/399 (78%), Gaps = 18/399 (4%)
Frame = +2
Query: 224 PLPTSWDPPRP----SSVSHAYADLEPYLG-----------CSMPGKR-LKVAVLLSGGV 355
PL PPRP SS S A LG C+ G+ L+VAVLLSGGV
Sbjct: 22 PLLCPRHPPRPFRALSSSSPVAASPPSALGVGDIREEHLVRCAAAGRAPLRVAVLLSGGV 81
Query: 356 DSSVALRLLHAAGHDITAFYLKIWFQEDFENFWSECPWDEDLKYATIVCDQVDVQFEVVH 535
DSSVALRLLHAAGH TAFYLKIWFQEDF NFWSECPWD+DLKYA VCD++DV EV H
Sbjct: 82 DSSVALRLLHAAGHSCTAFYLKIWFQEDFRNFWSECPWDDDLKYAQAVCDKIDVPLEVAH 141
Query: 536 LTDEYWKNVVSHIINEYRSGRTPNPDVLCNTRVKFGAFLEAIGSRGFDYVASGHYAHVIH 715
L+DEYW +VVSHIINEYR+GRTPNPDVLCNTR+KFGAFLEAI + GFDY+ASGHYAHV+H
Sbjct: 142 LSDEYWNHVVSHIINEYRNGRTPNPDVLCNTRIKFGAFLEAIDNLGFDYIASGHYAHVVH 201
Query: 716 SSSK--DGLSILKLSKDMVKDQTYFXXXXXXXXXXXXXFPLGCITKEEVRSIASLMDLPN 889
S + +G S+L+LSKD VKDQTYF FPLGCITK+EVRS+A+ MDLPN
Sbjct: 202 PSIENVEGPSVLQLSKDKVKDQTYFLSHLSQPQLRRLLFPLGCITKDEVRSLATQMDLPN 261
Query: 890 KDRKDSQGICFLGKVKFSEFVAKHIGEMEGIMLEAETGDYLGKHRGFWFYTIGQRQGLRL 1069
+ RKDSQGICFLGKVKFSEFV +HIGEMEG++LEAETGDYLG+HRGFWFYTIGQRQGLRL
Sbjct: 262 QGRKDSQGICFLGKVKFSEFVERHIGEMEGVLLEAETGDYLGRHRGFWFYTIGQRQGLRL 321
Query: 1070 PGGPWYVVEKDVQNNVLFVSRNYFSLNKRRLTFRVESLNWFSGSSPINVEQLQCKVRHGP 1249
PGGPWYVVEKDVQNNV+FVSRNY+SL+KRR TFRV SLNWFS S P N EQL+CKVRH P
Sbjct: 322 PGGPWYVVEKDVQNNVVFVSRNYYSLDKRRRTFRVGSLNWFSDSGPENDEQLKCKVRHSP 381
Query: 1250 GFYECSITSGSGISDEESLVVHLSEDDQGLAAGQFAAFY 1366
F+ C++T + L VHLSEDDQGLAAGQFAAFY
Sbjct: 382 EFHNCTVTREQTEESGDVLAVHLSEDDQGLAAGQFAAFY 420
>gb|EEC74610.1| hypothetical protein OsI_10221 [Oryza sativa Indica Group]
Length = 488
Score = 562 bits (1449), Expect = e-157
Identities = 277/381 (72%), Positives = 308/381 (80%), Gaps = 10/381 (2%)
Frame = +2
Query: 254 PSSVSHAYADL-------EPYLGCSMPGKR-LKVAVLLSGGVDSSVALRLLHAAGHDITA 409
PSS S D E L C+ G+ L+VAVL+SGGVDSSVALRLLHAAGH TA
Sbjct: 47 PSSSSSGARDFGGVDFGDERLLRCAAAGRAPLRVAVLVSGGVDSSVALRLLHAAGHRCTA 106
Query: 410 FYLKIWFQEDFENFWSECPWDEDLKYATIVCDQVDVQFEVVHLTDEYWKNVVSHIINEYR 589
FYLKIWFQEDF NFWSECPWD+DLKYA VCD++DV EVVHL+DEYW +VVSHIINEYR
Sbjct: 107 FYLKIWFQEDFRNFWSECPWDDDLKYAQAVCDKIDVPLEVVHLSDEYWNHVVSHIINEYR 166
Query: 590 SGRTPNPDVLCNTRVKFGAFLEAIGSRGFDYVASGHYAHVIHSSSKD--GLSILKLSKDM 763
SGRTPNPDVLCNTR+KFGAFLEAI + GFDY+ASGHYAHV+H S D G S+L+LSKD
Sbjct: 167 SGRTPNPDVLCNTRIKFGAFLEAIENLGFDYIASGHYAHVVHPSPDDVEGPSVLQLSKDK 226
Query: 764 VKDQTYFXXXXXXXXXXXXXFPLGCITKEEVRSIASLMDLPNKDRKDSQGICFLGKVKFS 943
VKDQTYF FPLGCITK+EVR +A+ MDLPN+DRKDSQGICFLGKVKFS
Sbjct: 227 VKDQTYFLSHLSQTQLRRLLFPLGCITKDEVRRLAAQMDLPNQDRKDSQGICFLGKVKFS 286
Query: 944 EFVAKHIGEMEGIMLEAETGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVLF 1123
EFV + IGEMEG++LEAETGDYLG HRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNV+F
Sbjct: 287 EFVERQIGEMEGVLLEAETGDYLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVVF 346
Query: 1124 VSRNYFSLNKRRLTFRVESLNWFSGSSPINVEQLQCKVRHGPGFYECSITSGSGISDEES 1303
VSRNY+SL+KRR TFRV SLNWFS S P N EQL+CKVRH P F++C++T +
Sbjct: 347 VSRNYYSLDKRRRTFRVGSLNWFSNSGPTNNEQLKCKVRHSPEFHDCTVTQEQTSENGVI 406
Query: 1304 LVVHLSEDDQGLAAGQFAAFY 1366
LVV LSEDDQGLAAGQFAAFY
Sbjct: 407 LVVRLSEDDQGLAAGQFAAFY 427
>ref|XP_004507093.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cicer arietinum]
Length = 484
Score = 561 bits (1446), Expect = e-157
Identities = 271/364 (74%), Positives = 303/364 (83%), Gaps = 3/364 (0%)
Frame = +2
Query: 284 LEPYLGCSMPGKRLKVAVLLSGGVDSSVALRLLHAAGHDITAFYLKIWFQEDFENFWSEC 463
L+PYL CSMP L+VAVL+SGGVDSSVALRLLHAAGH TAFYLKIWFQEDFENFWSEC
Sbjct: 56 LDPYLRCSMPHNPLRVAVLISGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSEC 115
Query: 464 PWDEDLKYATIVCDQVDVQFEVVHLTDEYWKNVVSHIINEYRSGRTPNPDVLCNTRVKFG 643
PW++DLKYA VC+QV+V EVVHLTDEYWKNVVS+II EY+ GRTPNPDVLCNTR+KFG
Sbjct: 116 PWEDDLKYAKDVCNQVEVPLEVVHLTDEYWKNVVSYIIEEYQCGRTPNPDVLCNTRIKFG 175
Query: 644 AFLEAIGSRGFDYVASGHYAHVIHSSSK--DGLSILKLSKDMVKDQTYFXXXXXXXXXXX 817
AFL+AI GFDYVASGHYA+VIH S+ DG S L+LS+DMVKDQTYF
Sbjct: 176 AFLDAISDMGFDYVASGHYANVIHPSADQMDGPSTLELSQDMVKDQTYFLSHLSQSQLKQ 235
Query: 818 XXFPLGCITKEEVRSIASLMDLPNKDRKDSQGICFLGKVKFSEFVAKHIGEMEGIMLEAE 997
FPLGCI K+EVR +A+ DLPNKDRKDSQGICFLGK+KFSEFVA+HIGE EGI+LEAE
Sbjct: 236 LLFPLGCIPKDEVRRLATKFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEKEGIILEAE 295
Query: 998 TGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVLFVSRNYFSLNKRRLTFRVE 1177
TGD+LGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDV+NNV+FVSRNYFS++KRR FRV
Sbjct: 296 TGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSIDKRRRVFRVG 355
Query: 1178 SLNWFSGSSPINVEQLQCKVRHGPGFYECSITSG-SGISDEESLVVHLSEDDQGLAAGQF 1354
SL W SG P QL+CKVRHGPGFY+CS+ G + +S VV +SEDDQGLAAGQF
Sbjct: 356 SLKWLSGLPPSQTSQLRCKVRHGPGFYDCSLQMELEGENPIDSAVVRISEDDQGLAAGQF 415
Query: 1355 AAFY 1366
AFY
Sbjct: 416 TAFY 419
>ref|XP_006649485.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like [Oryza
brachyantha]
Length = 489
Score = 560 bits (1442), Expect = e-157
Identities = 270/360 (75%), Positives = 302/360 (83%), Gaps = 3/360 (0%)
Frame = +2
Query: 296 LGCSMPGKR-LKVAVLLSGGVDSSVALRLLHAAGHDITAFYLKIWFQEDFENFWSECPWD 472
L C G+ L+VAVL+SGGVDSSVALRLLHAAGH TAFYLKIWFQEDF NFWSECPWD
Sbjct: 69 LRCVEAGRAPLRVAVLVSGGVDSSVALRLLHAAGHRCTAFYLKIWFQEDFRNFWSECPWD 128
Query: 473 EDLKYATIVCDQVDVQFEVVHLTDEYWKNVVSHIINEYRSGRTPNPDVLCNTRVKFGAFL 652
+DLKY VCD+++V EVVHL+DEYW +VVSHIINEYRSG TPNPDVLCNTR+KFGAFL
Sbjct: 129 DDLKYVQAVCDKINVPLEVVHLSDEYWNHVVSHIINEYRSGHTPNPDVLCNTRIKFGAFL 188
Query: 653 EAIGSRGFDYVASGHYAHVIHSS--SKDGLSILKLSKDMVKDQTYFXXXXXXXXXXXXXF 826
EAI + GFDY+ASGHYAHV+H S + +G S+L+LSKD VKDQTYF F
Sbjct: 189 EAIENMGFDYIASGHYAHVVHPSLDNVEGPSVLQLSKDKVKDQTYFLSHLSQTQLRRLLF 248
Query: 827 PLGCITKEEVRSIASLMDLPNKDRKDSQGICFLGKVKFSEFVAKHIGEMEGIMLEAETGD 1006
PLGCITK+EVR +A+ MDLPN+DRKDSQGICFLGKVKFSEFV +HIGEMEGI+LEAETGD
Sbjct: 249 PLGCITKDEVRRLAAQMDLPNQDRKDSQGICFLGKVKFSEFVERHIGEMEGILLEAETGD 308
Query: 1007 YLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVLFVSRNYFSLNKRRLTFRVESLN 1186
YLG HRGFWFYTIGQRQGLRLPGGPWYVVEKDV+NNV+FVSRNY+SL+KRR TFRV SLN
Sbjct: 309 YLGIHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSLDKRRRTFRVGSLN 368
Query: 1187 WFSGSSPINVEQLQCKVRHGPGFYECSITSGSGISDEESLVVHLSEDDQGLAAGQFAAFY 1366
WFS S PIN EQL+CKVRH P F+ C++T + LVVHLSEDDQGLAAGQFAAFY
Sbjct: 369 WFSNSGPINNEQLKCKVRHSPEFHHCTVTQEQTSENRVILVVHLSEDDQGLAAGQFAAFY 428
>ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cucumis sativus]
gi|449524848|ref|XP_004169433.1| PREDICTED: tRNA-specific
2-thiouridylase MnmA-like [Cucumis sativus]
Length = 538
Score = 560 bits (1442), Expect = e-157
Identities = 271/366 (74%), Positives = 302/366 (82%), Gaps = 3/366 (0%)
Frame = +2
Query: 278 ADLEPYLGCSMPGKRLKVAVLLSGGVDSSVALRLLHAAGHDITAFYLKIWFQEDFENFWS 457
AD P+L CSMP LKVAVLLSGGVDSSVALRLLHAAGH TAFYLKIWFQEDFENFWS
Sbjct: 67 ADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 126
Query: 458 ECPWDEDLKYATIVCDQVDVQFEVVHLTDEYWKNVVSHIINEYRSGRTPNPDVLCNTRVK 637
ECPWD+DLKYA VCDQVDV EVVHLT+EYW+NVVS+II EYR GRTPNPDVLCNTR+K
Sbjct: 127 ECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIK 186
Query: 638 FGAFLEAIGSRGFDYVASGHYAHVIHSSSK--DGLSILKLSKDMVKDQTYFXXXXXXXXX 811
FGAF++AI + FDYVASGHYA+VIH S+ D S+L+LSKDMVKDQTYF
Sbjct: 187 FGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQL 246
Query: 812 XXXXFPLGCITKEEVRSIASLMDLPNKDRKDSQGICFLGKVKFSEFVAKHIGEMEGIMLE 991
FPLGCI K+EVR +A+ +LPNKDRKDSQGICFLGK+KFSEFVA+HIGE EG++LE
Sbjct: 247 KRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILE 306
Query: 992 AETGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVLFVSRNYFSLNKRRLTFR 1171
AE+GD+LGKHRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNV+FVSRNYFS +KRR FR
Sbjct: 307 AESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFR 366
Query: 1172 VESLNWFSGSSPINVEQLQCKVRHGPGFYECSIT-SGSGISDEESLVVHLSEDDQGLAAG 1348
V SL W SG P +LQCKVRHGP FY C++T S E+ VVHLSEDDQGLAAG
Sbjct: 367 VGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQGLAAG 426
Query: 1349 QFAAFY 1366
QFAAFY
Sbjct: 427 QFAAFY 432
>ref|NP_001049129.1| Os03g0174600 [Oryza sativa Japonica Group]
gi|108706458|gb|ABF94253.1| tRNA methyl transferase
family protein, expressed [Oryza sativa Japonica Group]
gi|113547600|dbj|BAF11043.1| Os03g0174600 [Oryza sativa
Japonica Group] gi|215737060|dbj|BAG95989.1| unnamed
protein product [Oryza sativa Japonica Group]
Length = 488
Score = 559 bits (1440), Expect = e-156
Identities = 276/381 (72%), Positives = 306/381 (80%), Gaps = 10/381 (2%)
Frame = +2
Query: 254 PSSVSHAYADL-------EPYLGCSMPGKR-LKVAVLLSGGVDSSVALRLLHAAGHDITA 409
PSS S D E L C+ G+ L+VAVL+SGGVDSSVALRLLHAAGH TA
Sbjct: 47 PSSSSSGARDFGGVDFGDERLLRCAAAGRAPLRVAVLVSGGVDSSVALRLLHAAGHRCTA 106
Query: 410 FYLKIWFQEDFENFWSECPWDEDLKYATIVCDQVDVQFEVVHLTDEYWKNVVSHIINEYR 589
FYLKIWFQEDF NFWSECPWD+DLKYA VCD++DV EVVHL+DEYW +VVSHIINEYR
Sbjct: 107 FYLKIWFQEDFRNFWSECPWDDDLKYAQAVCDKIDVPLEVVHLSDEYWNHVVSHIINEYR 166
Query: 590 SGRTPNPDVLCNTRVKFGAFLEAIGSRGFDYVASGHYAHVIHSSSKD--GLSILKLSKDM 763
SGRTPNPDVLCNTR+KFGAFLEAI + GFDY+ASGHYAHV+H S D G S+L+LSKD
Sbjct: 167 SGRTPNPDVLCNTRIKFGAFLEAIENLGFDYIASGHYAHVVHPSPDDVEGPSVLQLSKDK 226
Query: 764 VKDQTYFXXXXXXXXXXXXXFPLGCITKEEVRSIASLMDLPNKDRKDSQGICFLGKVKFS 943
VKDQTYF FPLGCITK+EVR +A+ MDLPN+DRKDSQGICFLGKVKFS
Sbjct: 227 VKDQTYFLSHLSQTQLRRLLFPLGCITKDEVRRLAAQMDLPNQDRKDSQGICFLGKVKFS 286
Query: 944 EFVAKHIGEMEGIMLEAETGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVLF 1123
EFV + IGEMEG++LEAETGDYLG HRGFWFYTIGQRQGLRLPGGPWYVVEK VQNNV+F
Sbjct: 287 EFVERQIGEMEGVLLEAETGDYLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKGVQNNVVF 346
Query: 1124 VSRNYFSLNKRRLTFRVESLNWFSGSSPINVEQLQCKVRHGPGFYECSITSGSGISDEES 1303
VSRNY+SL+KRR TFRV SLNWFS S P N EQL+CKVRH P F++C++T
Sbjct: 347 VSRNYYSLDKRRRTFRVGSLNWFSNSGPTNNEQLKCKVRHSPEFHDCTVTQEQTSEHGVI 406
Query: 1304 LVVHLSEDDQGLAAGQFAAFY 1366
LVV LSEDDQGLAAGQFAAFY
Sbjct: 407 LVVRLSEDDQGLAAGQFAAFY 427
>ref|XP_002468413.1| hypothetical protein SORBIDRAFT_01g045520 [Sorghum bicolor]
gi|241922267|gb|EER95411.1| hypothetical protein
SORBIDRAFT_01g045520 [Sorghum bicolor]
Length = 489
Score = 557 bits (1435), Expect = e-156
Identities = 273/374 (72%), Positives = 309/374 (82%), Gaps = 4/374 (1%)
Frame = +2
Query: 257 SSVSHAYADLEPYLG-CSMPGKR-LKVAVLLSGGVDSSVALRLLHAAGHDITAFYLKIWF 430
+SV LE +L C+ G+ L+VAVL+SGGVDSSVALRLLHAAGH TAFYLKIWF
Sbjct: 54 ASVKGEDVALEEHLTRCAAAGRAPLRVAVLVSGGVDSSVALRLLHAAGHHCTAFYLKIWF 113
Query: 431 QEDFENFWSECPWDEDLKYATIVCDQVDVQFEVVHLTDEYWKNVVSHIINEYRSGRTPNP 610
QEDF NFWSECPWDEDLKYA VCD++DV EVVHL+DEYW VVSH+INEYR GRTPNP
Sbjct: 114 QEDFRNFWSECPWDEDLKYAQAVCDKIDVPLEVVHLSDEYWNYVVSHMINEYRCGRTPNP 173
Query: 611 DVLCNTRVKFGAFLEAIGSRGFDYVASGHYAHVIHSSSK--DGLSILKLSKDMVKDQTYF 784
DVLCNTR+KFGAFLEAI + GFDY+ASGHYAHV+H S++ + SIL+LSKD VKDQTYF
Sbjct: 174 DVLCNTRIKFGAFLEAIENLGFDYIASGHYAHVVHPSTENTEAPSILQLSKDKVKDQTYF 233
Query: 785 XXXXXXXXXXXXXFPLGCITKEEVRSIASLMDLPNKDRKDSQGICFLGKVKFSEFVAKHI 964
FPLGCITK+EVR +A+ M LPN+ RKDSQGICFLGKVKFSEFV +HI
Sbjct: 234 LSHLSQPQLRRLLFPLGCITKDEVRRLATQMGLPNQGRKDSQGICFLGKVKFSEFVERHI 293
Query: 965 GEMEGIMLEAETGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVLFVSRNYFS 1144
GEMEGI+LEAE+GDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNV+FVSRNY+S
Sbjct: 294 GEMEGIILEAESGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVVFVSRNYYS 353
Query: 1145 LNKRRLTFRVESLNWFSGSSPINVEQLQCKVRHGPGFYECSITSGSGISDEESLVVHLSE 1324
L+KRR TFRV SLNWF S P N EQL+CKVRH P F++C++ +E++LVVHLSE
Sbjct: 354 LDKRRRTFRVGSLNWFDSSGPGNNEQLKCKVRHSPEFHDCTLIKEHTEENEDALVVHLSE 413
Query: 1325 DDQGLAAGQFAAFY 1366
DDQGLAAGQFAAFY
Sbjct: 414 DDQGLAAGQFAAFY 427
>ref|XP_003539065.2| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like isoform
X1 [Glycine max]
Length = 502
Score = 556 bits (1432), Expect = e-155
Identities = 273/381 (71%), Positives = 306/381 (80%), Gaps = 8/381 (2%)
Frame = +2
Query: 248 PRPSSVSHAY----ADLEPYLGCSMP-GKRLKVAVLLSGGVDSSVALRLLHAAGHDITAF 412
P S+V+H DL+ YL CSMP L+VAVL+SGGVDSSVALRLLHAAGH TAF
Sbjct: 46 PSSSAVAHTSPPLSGDLDRYLRCSMPQNSPLRVAVLVSGGVDSSVALRLLHAAGHSCTAF 105
Query: 413 YLKIWFQEDFENFWSECPWDEDLKYATIVCDQVDVQFEVVHLTDEYWKNVVSHIINEYRS 592
YLKIWFQEDFENFWSECPW++DLKYA VC+QVDV EVVHLTDEYW NVVS++I EY S
Sbjct: 106 YLKIWFQEDFENFWSECPWEDDLKYAKDVCNQVDVPLEVVHLTDEYWNNVVSYLIEEYSS 165
Query: 593 GRTPNPDVLCNTRVKFGAFLEAIGSRGFDYVASGHYAHVIH--SSSKDGLSILKLSKDMV 766
GRTPNPDVLCNTR+KFGAFL+AIG GFDYVASGHYA+VIH S +D S+L+LS+DMV
Sbjct: 166 GRTPNPDVLCNTRIKFGAFLDAIGGMGFDYVASGHYANVIHPCSDRRDEPSVLELSQDMV 225
Query: 767 KDQTYFXXXXXXXXXXXXXFPLGCITKEEVRSIASLMDLPNKDRKDSQGICFLGKVKFSE 946
KDQTYF FPLGCI K+EVR +A+ DLPNKDRKDSQGICFLGK++FSE
Sbjct: 226 KDQTYFLSHLSQSQLKQLLFPLGCIPKDEVRKLATKFDLPNKDRKDSQGICFLGKIRFSE 285
Query: 947 FVAKHIGEMEGIMLEAETGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVLFV 1126
FVA+HIGE EG++LEAETGD+LGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDV+NNV+FV
Sbjct: 286 FVARHIGEREGVILEAETGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFV 345
Query: 1127 SRNYFSLNKRRLTFRVESLNWFSGSSPINVEQLQCKVRHGPGFYECSITSGSGISDE-ES 1303
SRNYFS +KRR FRV S W SG P QLQCKVRHGPGFY+CS+ + S
Sbjct: 346 SRNYFSFDKRRRVFRVGSFKWLSGLPPGQTTQLQCKVRHGPGFYDCSLQMEVEADGQCHS 405
Query: 1304 LVVHLSEDDQGLAAGQFAAFY 1366
VV +SEDDQGLAAGQFAAFY
Sbjct: 406 AVVRISEDDQGLAAGQFAAFY 426
>ref|XP_006470603.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like [Citrus
sinensis]
Length = 525
Score = 556 bits (1432), Expect = e-155
Identities = 275/379 (72%), Positives = 307/379 (81%), Gaps = 6/379 (1%)
Frame = +2
Query: 248 PRPSSVSHAYADLEP-YLGCSMPGKR-LKVAVLLSGGVDSSVALRLLHAAGHDITAFYLK 421
P SVS A +EP YL CSMP ++ LKVAVLLSGGVDSSVALRLLH+AGH TAFYLK
Sbjct: 43 PISCSVSTAATQIEPRYLSCSMPHQKGLKVAVLLSGGVDSSVALRLLHSAGHSCTAFYLK 102
Query: 422 IWFQEDFENFWSECPWDEDLKYATIVCDQVDVQFEVVHLTDEYWKNVVSHIINEYRSGRT 601
IWFQEDFENFW+ECPW+ED+KYA VCDQVDV EVVHLTDEYWKNVVS+II EYR GRT
Sbjct: 103 IWFQEDFENFWTECPWEEDMKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRT 162
Query: 602 PNPDVLCNTRVKFGAFLEAIGSRGFDYVASGHYAHVIHSSSKDGL--SILKLSKDMVKDQ 775
PNPDVLCNTR+KFGAF++AI S FDYVASGHYA V+HSS+ S+L+LSKD VKDQ
Sbjct: 163 PNPDVLCNTRIKFGAFMDAISSMEFDYVASGHYAKVVHSSADQEYKPSVLELSKDKVKDQ 222
Query: 776 TYFXXXXXXXXXXXXXFPLGCITKEEVRSIASLMDLPNKDRKDSQGICFLGKVKFSEFVA 955
TYF FPLGCI+KEEVR A+ DLPNKDRKDSQGICFLGK+KFSEFVA
Sbjct: 223 TYFLSHLSQAQLKRLIFPLGCISKEEVRKFATQFDLPNKDRKDSQGICFLGKIKFSEFVA 282
Query: 956 KHIGEMEGIMLEAETGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVLFVSRN 1135
+HIGEMEG++LEAETGD+LGKH GFWFYTIGQRQGLRLPGGPWYVVEKDV+NNV+FVSRN
Sbjct: 283 RHIGEMEGVILEAETGDFLGKHWGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRN 342
Query: 1136 YFSLNKRRLTFRVESLNWFSGSSPINVEQLQCKVRHGPGFYECSITSGSGISD--EESLV 1309
Y+S +K+R FRV SL W SG P + +LQCKVRHGPGFY C++ + D E V
Sbjct: 343 YYSFDKKRRLFRVGSLKWLSGLPPEEISRLQCKVRHGPGFYNCNLMI-EHVEDGGENIAV 401
Query: 1310 VHLSEDDQGLAAGQFAAFY 1366
V LSEDDQGLAAGQFAAFY
Sbjct: 402 VQLSEDDQGLAAGQFAAFY 420
>gb|ESW04011.1| hypothetical protein PHAVU_011G059600g [Phaseolus vulgaris]
Length = 488
Score = 556 bits (1432), Expect = e-155
Identities = 274/381 (71%), Positives = 305/381 (80%), Gaps = 8/381 (2%)
Frame = +2
Query: 248 PRPSSVSHAY----ADLEPYLGCSMP-GKRLKVAVLLSGGVDSSVALRLLHAAGHDITAF 412
P S+V+H ADL YL CS+P L+VAVL+SGGVDSSVALRLLHAAGH TAF
Sbjct: 44 PSSSAVAHTSLPLSADLHRYLRCSLPQNSPLRVAVLVSGGVDSSVALRLLHAAGHSCTAF 103
Query: 413 YLKIWFQEDFENFWSECPWDEDLKYATIVCDQVDVQFEVVHLTDEYWKNVVSHIINEYRS 592
YLKIWFQEDFENFWSECPW+EDLKYA VC+QVDV EVVHLTDEYW NVVS++I EY S
Sbjct: 104 YLKIWFQEDFENFWSECPWEEDLKYAKAVCEQVDVPLEVVHLTDEYWNNVVSYLIEEYSS 163
Query: 593 GRTPNPDVLCNTRVKFGAFLEAIGSRGFDYVASGHYAHVIH--SSSKDGLSILKLSKDMV 766
GRTPNPDVLCNTR+KFGAFL+AIG GFDYVASGHYA+VIH + +G S+L+LS+DMV
Sbjct: 164 GRTPNPDVLCNTRIKFGAFLDAIGGMGFDYVASGHYANVIHPCADGMNGPSVLELSQDMV 223
Query: 767 KDQTYFXXXXXXXXXXXXXFPLGCITKEEVRSIASLMDLPNKDRKDSQGICFLGKVKFSE 946
KDQTYF FPLGCI K+EVR +A+ DLPNKDRKDSQGICFLGK++FSE
Sbjct: 224 KDQTYFLSHLSQSQLKQLLFPLGCIPKDEVRRLATEFDLPNKDRKDSQGICFLGKIRFSE 283
Query: 947 FVAKHIGEMEGIMLEAETGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVLFV 1126
FVA+HIGE EGI+LEAETGD+LGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDV+NNV+FV
Sbjct: 284 FVARHIGEREGIILEAETGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFV 343
Query: 1127 SRNYFSLNKRRLTFRVESLNWFSGSSPINVEQLQCKVRHGPGFYECSI-TSGSGISDEES 1303
SRNYFS +KRR FRV S W S P QLQCKVRHGPGFY+CS+ G S
Sbjct: 344 SRNYFSFDKRRRVFRVGSFKWLSELPPGQTSQLQCKVRHGPGFYDCSLQMEVEGDGQGHS 403
Query: 1304 LVVHLSEDDQGLAAGQFAAFY 1366
VV +SEDDQGLAAGQFAAFY
Sbjct: 404 AVVRISEDDQGLAAGQFAAFY 424
>ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
putative [Ricinus communis] gi|223547418|gb|EEF48913.1|
tRNA
(5-methylaminomethyl-2-thiouridylate)-methyltransferase,
putative [Ricinus communis]
Length = 497
Score = 555 bits (1430), Expect = e-155
Identities = 269/366 (73%), Positives = 303/366 (82%), Gaps = 5/366 (1%)
Frame = +2
Query: 284 LEP-YLGCSMPGKR-LKVAVLLSGGVDSSVALRLLHAAGHDITAFYLKIWFQEDFENFWS 457
+EP YL C +P K L++A+LLSGGVDSSVALRLLHAAGH TAFYLKIWFQEDFENFWS
Sbjct: 56 IEPNYLSCCLPDKNNLRIALLLSGGVDSSVALRLLHAAGHHCTAFYLKIWFQEDFENFWS 115
Query: 458 ECPWDEDLKYATIVCDQVDVQFEVVHLTDEYWKNVVSHIINEYRSGRTPNPDVLCNTRVK 637
ECPW+EDLKYA VCDQVD+ EVVHLTDEYWKNVVS+II EYR GRTPNPDVLCNTR+K
Sbjct: 116 ECPWEEDLKYAKAVCDQVDIPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIK 175
Query: 638 FGAFLEAIGSRGFDYVASGHYAHVIHSSSKD--GLSILKLSKDMVKDQTYFXXXXXXXXX 811
FGAF++AI + FDYVASGHYA VIH S+ + S+L+LSKDMVKDQTYF
Sbjct: 176 FGAFMDAINNMEFDYVASGHYAKVIHPSADEINQPSVLELSKDMVKDQTYFLSHLSQAQL 235
Query: 812 XXXXFPLGCITKEEVRSIASLMDLPNKDRKDSQGICFLGKVKFSEFVAKHIGEMEGIMLE 991
FPLG I+K+EVR +A+L +LPNKDRKDSQGICFLGK+KFSEFVA+HIGEMEGIMLE
Sbjct: 236 RRLIFPLGGISKDEVRKLATLFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGIMLE 295
Query: 992 AETGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVLFVSRNYFSLNKRRLTFR 1171
AETGD+LG HRGFWFYTIGQRQGLRLPGGPWYVVEKDV+NNV+FVSRNYFS +KRR R
Sbjct: 296 AETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRLLR 355
Query: 1172 VESLNWFSGSSPINVEQLQCKVRHGPGFYECSITSGSG-ISDEESLVVHLSEDDQGLAAG 1348
V SL WF+GS P +LQCKVRHGPGFY CS+ G + D++ +V L EDDQGLAAG
Sbjct: 356 VGSLRWFNGSPPKETSELQCKVRHGPGFYNCSVVIEPGEVDDQDVAIVQLCEDDQGLAAG 415
Query: 1349 QFAAFY 1366
QFAAFY
Sbjct: 416 QFAAFY 421
>gb|EMT11416.1| tRNA-specific 2-thiouridylase mnmA [Aegilops tauschii]
Length = 552
Score = 553 bits (1424), Expect = e-155
Identities = 273/390 (70%), Positives = 311/390 (79%), Gaps = 4/390 (1%)
Frame = +2
Query: 209 VDTVAPLPTSWDPPRPSSVSHAYADLEPYLGCSMPGKR--LKVAVLLSGGVDSSVALRLL 382
+ + APL S P PS A E +L +R L+VAVLLSGGVDSSVALRLL
Sbjct: 36 LSSAAPLAAS-PPSAPSPAPGAVEFREEHLVRCAAARRAPLRVAVLLSGGVDSSVALRLL 94
Query: 383 HAAGHDITAFYLKIWFQEDFENFWSECPWDEDLKYATIVCDQVDVQFEVVHLTDEYWKNV 562
HAAGH TAFYLKIWFQEDF NFWSECPWD+DLKYA VCD++DV EVVHL+DEYW +V
Sbjct: 95 HAAGHSCTAFYLKIWFQEDFRNFWSECPWDDDLKYAQAVCDKIDVPLEVVHLSDEYWNHV 154
Query: 563 VSHIINEYRSGRTPNPDVLCNTRVKFGAFLEAIGSRGFDYVASGHYAHVIHS--SSKDGL 736
VSH+INEYR+GRTPNPDVLCNTR+KFGAFLEAI + GFDY+ASGHYAHV+H + +G
Sbjct: 155 VSHMINEYRNGRTPNPDVLCNTRIKFGAFLEAIENLGFDYIASGHYAHVVHPPFENAEGP 214
Query: 737 SILKLSKDMVKDQTYFXXXXXXXXXXXXXFPLGCITKEEVRSIASLMDLPNKDRKDSQGI 916
S+L+LSKD VKDQTYF FPLGCITK+EVRS+A MDLPN+ RKDSQGI
Sbjct: 215 SVLQLSKDEVKDQTYFLSHLSQPQLRRLLFPLGCITKDEVRSLAVQMDLPNQARKDSQGI 274
Query: 917 CFLGKVKFSEFVAKHIGEMEGIMLEAETGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVE 1096
CFLGKVKFSEFV +HIGEM+GI+LEAETGDYLG HRGFWFYTIGQRQGLRL GGPWYVVE
Sbjct: 275 CFLGKVKFSEFVQRHIGEMKGILLEAETGDYLGMHRGFWFYTIGQRQGLRLSGGPWYVVE 334
Query: 1097 KDVQNNVLFVSRNYFSLNKRRLTFRVESLNWFSGSSPINVEQLQCKVRHGPGFYECSITS 1276
KDVQNNV+FVSRNY+SL+KRR TFRV SLNWFS S P + E+L+CKVRH P F++C++T
Sbjct: 335 KDVQNNVVFVSRNYYSLDKRRRTFRVGSLNWFSDSGPSDNERLKCKVRHSPDFHDCTVTK 394
Query: 1277 GSGISDEESLVVHLSEDDQGLAAGQFAAFY 1366
+ + L V LSEDDQGLAAGQFAAFY
Sbjct: 395 EQTEENGDVLAVRLSEDDQGLAAGQFAAFY 424
>ref|XP_004985520.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like
[Setaria italica]
Length = 488
Score = 547 bits (1409), Expect = e-153
Identities = 261/350 (74%), Positives = 295/350 (84%), Gaps = 2/350 (0%)
Frame = +2
Query: 323 LKVAVLLSGGVDSSVALRLLHAAGHDITAFYLKIWFQEDFENFWSECPWDEDLKYATIVC 502
L+VAVL+SGGVDSSVALRLLHAAGH TAFYLKIWFQEDF NFWSECPWDEDLKYA +VC
Sbjct: 78 LRVAVLVSGGVDSSVALRLLHAAGHRCTAFYLKIWFQEDFRNFWSECPWDEDLKYAQVVC 137
Query: 503 DQVDVQFEVVHLTDEYWKNVVSHIINEYRSGRTPNPDVLCNTRVKFGAFLEAIGSRGFDY 682
D++DV EVVHL+DEYW +VVSHIINEYR GRTPNPDVLCNTR+KFGAFLEAI + GFDY
Sbjct: 138 DKIDVPLEVVHLSDEYWNHVVSHIINEYRCGRTPNPDVLCNTRIKFGAFLEAIENLGFDY 197
Query: 683 VASGHYAHVIHSS--SKDGLSILKLSKDMVKDQTYFXXXXXXXXXXXXXFPLGCITKEEV 856
+ASGHYAHV+H S + + S+L+LSKD +KDQTYF FPLGCI K+EV
Sbjct: 198 IASGHYAHVVHPSVENTEAPSVLQLSKDKIKDQTYFLSHLSQAQLRRLLFPLGCIKKDEV 257
Query: 857 RSIASLMDLPNKDRKDSQGICFLGKVKFSEFVAKHIGEMEGIMLEAETGDYLGKHRGFWF 1036
R +A+ MDLPN+ RKDSQGICFLGKVKFSEFV +HIGEMEGI+LEAE+GDYLG HRGFWF
Sbjct: 258 RRLAAQMDLPNQGRKDSQGICFLGKVKFSEFVERHIGEMEGIILEAESGDYLGNHRGFWF 317
Query: 1037 YTIGQRQGLRLPGGPWYVVEKDVQNNVLFVSRNYFSLNKRRLTFRVESLNWFSGSSPINV 1216
YTIGQRQGLRL GGPWYVVEKDVQNNV+FVSRNY+SL+KRR TFRV SLNWF S P N
Sbjct: 318 YTIGQRQGLRLAGGPWYVVEKDVQNNVVFVSRNYYSLDKRRRTFRVGSLNWFDDSGPGNS 377
Query: 1217 EQLQCKVRHGPGFYECSITSGSGISDEESLVVHLSEDDQGLAAGQFAAFY 1366
E+L+CKVRH P F++C++T + + LVV LSEDDQGLAAGQFAAFY
Sbjct: 378 ERLKCKVRHSPEFHDCTVTKEHTEENGDVLVVRLSEDDQGLAAGQFAAFY 427
>ref|XP_006358437.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like
[Solanum tuberosum]
Length = 488
Score = 546 bits (1407), Expect = e-153
Identities = 270/392 (68%), Positives = 307/392 (78%), Gaps = 6/392 (1%)
Frame = +2
Query: 209 VDTVAPLPTSWDPPRPSSVSHAYAD--LEP-YLGCSMPGKR-LKVAVLLSGGVDSSVALR 376
+ T+A S P SS Y D + P YL CSMP K LKVAVL+SGGVDSSVALR
Sbjct: 42 ITTIARRLFSLKPLAVSSPIRNYGDEKINPTYLSCSMPHKNPLKVAVLVSGGVDSSVALR 101
Query: 377 LLHAAGHDITAFYLKIWFQEDFENFWSECPWDEDLKYATIVCDQVDVQFEVVHLTDEYWK 556
LLHAAGH TAFYLKIWFQEDFEN+WSECPW++DLKYA VCDQVDV EVVHLTDEYW
Sbjct: 102 LLHAAGHSCTAFYLKIWFQEDFENYWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWN 161
Query: 557 NVVSHIINEYRSGRTPNPDVLCNTRVKFGAFLEAIGSRGFDYVASGHYAHVIHSSSK--D 730
NVVS+II EY+ GRTPNPDVLCNTR+KFGAF++AI FD+VASGHYA ++H+S+ D
Sbjct: 162 NVVSYIIEEYKCGRTPNPDVLCNTRIKFGAFMDAISGMEFDFVASGHYAKIVHASTAQLD 221
Query: 731 GLSILKLSKDMVKDQTYFXXXXXXXXXXXXXFPLGCITKEEVRSIASLMDLPNKDRKDSQ 910
SILKLSKDMVKDQTYF FPLGCI K+EVR++A +LPN+DRKDSQ
Sbjct: 222 EPSILKLSKDMVKDQTYFLSHLSQAQLKRLIFPLGCIQKDEVRTLAKSFNLPNQDRKDSQ 281
Query: 911 GICFLGKVKFSEFVAKHIGEMEGIMLEAETGDYLGKHRGFWFYTIGQRQGLRLPGGPWYV 1090
GICFLGK+KFSEFVA+HIGE EGI+LEAE GDYLG HRGFWFYTIGQRQGLRLPGGPWYV
Sbjct: 282 GICFLGKIKFSEFVARHIGESEGIILEAENGDYLGNHRGFWFYTIGQRQGLRLPGGPWYV 341
Query: 1091 VEKDVQNNVLFVSRNYFSLNKRRLTFRVESLNWFSGSSPINVEQLQCKVRHGPGFYECSI 1270
VEKD++NNV++VSRNYFS++K+R FRV SL W SG P + +LQCKVRHGP FY+CS+
Sbjct: 342 VEKDIKNNVVYVSRNYFSVDKKRRLFRVGSLKWLSGLFPKQINELQCKVRHGPSFYDCSL 401
Query: 1271 TSGSGISDEESLVVHLSEDDQGLAAGQFAAFY 1366
+E VV LS DDQGLAAGQFAAFY
Sbjct: 402 VMEVDQHGQEVAVVRLSGDDQGLAAGQFAAFY 433
>ref|XP_006850208.1| hypothetical protein AMTR_s00022p00251260 [Amborella trichopoda]
gi|548853806|gb|ERN11789.1| hypothetical protein
AMTR_s00022p00251260 [Amborella trichopoda]
Length = 565
Score = 546 bits (1406), Expect = e-152
Identities = 260/370 (70%), Positives = 302/370 (81%), Gaps = 3/370 (0%)
Frame = +2
Query: 266 SHAYADLEPYLGCSMPGKRLKVAVLLSGGVDSSVALRLLHAAGHDITAFYLKIWFQEDFE 445
S ++ + YL CS+P KR KVAVL+SGGVDSSVALRLL AGH TAFYLKIWFQEDFE
Sbjct: 105 SGSFVPEKRYLECSLPEKRSKVAVLVSGGVDSSVALRLLCYAGHSCTAFYLKIWFQEDFE 164
Query: 446 NFWSECPWDEDLKYATIVCDQVDVQFEVVHLTDEYWKNVVSHIINEYRSGRTPNPDVLCN 625
NFWS CPWDEDLKYA VCD+V V EVVHLTDEYW NVVSHII+EYRSGRTPNPDVLCN
Sbjct: 165 NFWSACPWDEDLKYAKAVCDEVHVPLEVVHLTDEYWNNVVSHIISEYRSGRTPNPDVLCN 224
Query: 626 TRVKFGAFLEAIGSRGFDYVASGHYAHVIHS--SSKDGLSILKLSKDMVKDQTYFXXXXX 799
TR+KFGAFL+AIG+ FDYVASGHYA V+H D S L+LS D VKDQTYF
Sbjct: 225 TRIKFGAFLDAIGNMDFDYVASGHYARVVHPPPDQPDASSALELSADKVKDQTYFLSHLS 284
Query: 800 XXXXXXXXFPLGCITKEEVRSIASLMDLPNKDRKDSQGICFLGKVKFSEFVAKHIGEMEG 979
FPLGC++K+EVR +A+ M LPNKDRKDSQGICFLGK+KFS+FV +HIGEMEG
Sbjct: 285 QTQLNRLLFPLGCLSKDEVRRLAAKMGLPNKDRKDSQGICFLGKLKFSDFVTRHIGEMEG 344
Query: 980 IMLEAETGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVLFVSRNYFSLNKRR 1159
++LEAETGD+LGKH+G+WFYTIGQRQGLRLPGGPWYVV KD++NNV+FVSRNYFS +K+R
Sbjct: 345 VLLEAETGDFLGKHQGYWFYTIGQRQGLRLPGGPWYVVNKDIENNVIFVSRNYFSFDKKR 404
Query: 1160 LTFRVESLNWFSGSSPINVEQLQCKVRHGPGFYECSITSGSGISD-EESLVVHLSEDDQG 1336
+FRV SL W SG+ P +++QLQCKVRHGP FY+C+ S + E+S ++H+SEDDQG
Sbjct: 405 RSFRVGSLKWLSGAPPEHLDQLQCKVRHGPCFYDCTFKIESRCDEHEDSALIHISEDDQG 464
Query: 1337 LAAGQFAAFY 1366
LAAGQFAAFY
Sbjct: 465 LAAGQFAAFY 474
>ref|NP_001131207.1| uncharacterized protein LOC100192515 [Zea mays]
gi|194690872|gb|ACF79520.1| unknown [Zea mays]
gi|414865088|tpg|DAA43645.1| TPA: tRNA methyltransferase
[Zea mays]
Length = 486
Score = 544 bits (1402), Expect = e-152
Identities = 258/350 (73%), Positives = 295/350 (84%), Gaps = 2/350 (0%)
Frame = +2
Query: 323 LKVAVLLSGGVDSSVALRLLHAAGHDITAFYLKIWFQEDFENFWSECPWDEDLKYATIVC 502
L+VAVL+SGGVDSSVALRLLHAAGH TAFYLKIWFQEDF NFWSECPWDEDLKYA VC
Sbjct: 75 LRVAVLVSGGVDSSVALRLLHAAGHRCTAFYLKIWFQEDFRNFWSECPWDEDLKYAQAVC 134
Query: 503 DQVDVQFEVVHLTDEYWKNVVSHIINEYRSGRTPNPDVLCNTRVKFGAFLEAIGSRGFDY 682
D++DV EVVHL+DEYW +VVSH+INEYR GRTPNPDVLCNTR+KFG+FLEAI + GFDY
Sbjct: 135 DKIDVPLEVVHLSDEYWNHVVSHMINEYRCGRTPNPDVLCNTRIKFGSFLEAIENLGFDY 194
Query: 683 VASGHYAHVIHSSSK--DGLSILKLSKDMVKDQTYFXXXXXXXXXXXXXFPLGCITKEEV 856
+ASGHYAHV+H S++ + S+L+LSKD +KDQTYF FPLGCITK+EV
Sbjct: 195 IASGHYAHVVHPSAENTEAPSVLQLSKDKIKDQTYFLSHLSQSQLRRLLFPLGCITKDEV 254
Query: 857 RSIASLMDLPNKDRKDSQGICFLGKVKFSEFVAKHIGEMEGIMLEAETGDYLGKHRGFWF 1036
R +A+ M LPN+ RKDSQGICFLGKVKFSEFV +HIGE EGI+LEAE+GDYLG HRGFWF
Sbjct: 255 RRLATQMGLPNQGRKDSQGICFLGKVKFSEFVERHIGEKEGIILEAESGDYLGIHRGFWF 314
Query: 1037 YTIGQRQGLRLPGGPWYVVEKDVQNNVLFVSRNYFSLNKRRLTFRVESLNWFSGSSPINV 1216
YTIGQRQGLRLPGGPWYVVEKDVQNNV+FVSRNY+SL+KRR TFRV SLNWF P N
Sbjct: 315 YTIGQRQGLRLPGGPWYVVEKDVQNNVVFVSRNYYSLDKRRRTFRVGSLNWFDNCGPGNN 374
Query: 1217 EQLQCKVRHGPGFYECSITSGSGISDEESLVVHLSEDDQGLAAGQFAAFY 1366
EQL+CKVRH P F++C++ + ++LVVHL+EDDQGLAAGQFAAFY
Sbjct: 375 EQLKCKVRHSPEFHDCTLVKERSEENGDALVVHLAEDDQGLAAGQFAAFY 424
>dbj|BAK01046.1| predicted protein [Hordeum vulgare subsp. vulgare]
Length = 491
Score = 544 bits (1401), Expect = e-152
Identities = 267/388 (68%), Positives = 307/388 (79%), Gaps = 9/388 (2%)
Frame = +2
Query: 230 PTSWDPPRPSSVSHAYADLEPYLGCSMPGKR-LKVAVLLSGGVDSSVALRLLHAAGHDIT 406
P + PP + A E + C+ G+ L+VAVLLSGGVDSSVALRLLHAAGH T
Sbjct: 43 PLAGSPPSAPAPGEADISEEHLVRCAAAGRAPLRVAVLLSGGVDSSVALRLLHAAGHSCT 102
Query: 407 AFYLKIWFQEDFENFWSECPWDEDLKYATIVCDQVDVQFEVVHLTDEYWKNVVSHIINEY 586
AFYLKIWFQEDF NFWSECPWD+DLKYA VCD++DV EVVHL+DEYW +VVSH+I+EY
Sbjct: 103 AFYLKIWFQEDFRNFWSECPWDDDLKYAQAVCDKIDVPLEVVHLSDEYWNHVVSHMISEY 162
Query: 587 RSGRTPNPDVLCNTRVKFGAFLEAIGSRGFDYVASGHYAHVIHS--SSKDGLSILKLSKD 760
R+GRTPNPDVLCNTR+KFGAFLEAI + GFDY+ASGHYAHV+H + +G S+L+LSKD
Sbjct: 163 RNGRTPNPDVLCNTRIKFGAFLEAIENLGFDYIASGHYAHVVHPPVENAEGPSVLQLSKD 222
Query: 761 MVKDQTYFXXXXXXXXXXXXXFPLGCITKEEVRSIASLMDLPNKDRKDSQGICFLGKVKF 940
VKDQTYF FPLGCITK+EVRS+A M+LPN+ RKDSQGICFLGKVKF
Sbjct: 223 EVKDQTYFLSHLSQSQLRRLLFPLGCITKDEVRSLAVQMNLPNQARKDSQGICFLGKVKF 282
Query: 941 SEFVAKHIGEMEGIMLEAETGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVL 1120
SEFV HIGE+EGI+LEAETGDYLG HRGFWFYTIGQRQGLRL GGPWYVVEKDVQNNV+
Sbjct: 283 SEFVQAHIGEIEGILLEAETGDYLGTHRGFWFYTIGQRQGLRLSGGPWYVVEKDVQNNVV 342
Query: 1121 FVSRNYFSLNKRRLTFRVESLNWFSGSSPINVEQLQCKVRHGPGFYECSITSGS------ 1282
FVSRNY+SL+KRR TFRV SLNWFS S P + E+L+CKVRH P F+ C++T
Sbjct: 343 FVSRNYYSLDKRRRTFRVGSLNWFSDSGPADNERLKCKVRHSPDFHNCTVTREQTEELVV 402
Query: 1283 GISDEESLVVHLSEDDQGLAAGQFAAFY 1366
++ + L V LSEDDQGLAAGQFAAFY
Sbjct: 403 NSNNGDVLAVRLSEDDQGLAAGQFAAFY 430