BLASTX nr result
ID: Zingiber23_contig00014210
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Zingiber23_contig00014210
(396 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
tpg|DAA53158.1| TPA: hypothetical protein ZEAMMB73_022786 [Zea m... 55 8e-11
gb|ACM66950.1| flavonoid glucosyltransferase [Crocus sativus] 50 1e-10
ref|XP_004965984.1| PREDICTED: UDP-glycosyltransferase 75D1-like... 52 1e-09
ref|NP_001057977.1| Os06g0593800 [Oryza sativa Japonica Group] g... 55 2e-09
gb|EEE65963.1| hypothetical protein OsJ_21854 [Oryza sativa Japo... 55 2e-09
ref|XP_002438626.1| hypothetical protein SORBIDRAFT_10g023070 [S... 51 3e-09
ref|XP_006657100.1| PREDICTED: UDP-glycosyltransferase 75D1-like... 57 5e-09
ref|XP_002263532.1| PREDICTED: UDP-glycosyltransferase 75C1-like... 55 2e-07
gb|ACD03260.1| UDP-glycosyltransferase UGT75E2 [Avena strigosa] 53 3e-07
ref|XP_003566479.1| PREDICTED: UDP-glycosyltransferase 75D1-like... 41 4e-07
ref|XP_003560622.1| PREDICTED: UDP-glycosyltransferase 75B1-like... 50 4e-07
dbj|BAJ96117.1| predicted protein [Hordeum vulgare subsp. vulgare] 59 9e-07
sp|Q767C8.1|IH5GT_IRIHO RecName: Full=Cyanidin 3-O-rutinoside 5-... 58 1e-06
ref|XP_003560621.1| PREDICTED: UDP-glycosyltransferase 75D1-like... 48 1e-06
dbj|BAJ90555.1| predicted protein [Hordeum vulgare subsp. vulgare] 50 2e-06
ref|XP_006664328.1| PREDICTED: cyanidin 3-O-rutinoside 5-O-gluco... 57 3e-06
emb|CBX25382.1| hypothetical_protein [Oryza brachyantha] 57 3e-06
ref|XP_003577063.1| PREDICTED: UDP-glycosyltransferase 75D1-like... 47 4e-06
gb|EMT24761.1| Indole-3-acetate beta-glucosyltransferase 1 [Aegi... 56 4e-06
ref|NP_001065729.1| Os11g0145200 [Oryza sativa Japonica Group] g... 56 4e-06
>tpg|DAA53158.1| TPA: hypothetical protein ZEAMMB73_022786 [Zea mays]
Length = 488
Score = 55.1 bits (131), Expect(2) = 8e-11
Identities = 28/59 (47%), Positives = 39/59 (66%), Gaps = 8/59 (13%)
Frame = +1
Query: 244 IGPVMS-------VNGPN-LFELDEQRYMEWLDSKAEGSVVYVSFGSFTTFKKRQAEEI 396
+GPV+S G N LF D + Y+EWLD++A GSVVY+SFGS + ++RQ EE+
Sbjct: 264 VGPVLSFLDDDAAAGGNNDLFTQDGKGYLEWLDAQAPGSVVYISFGSLSVMRRRQIEEV 322
Score = 37.0 bits (84), Expect(2) = 8e-11
Identities = 18/46 (39%), Positives = 26/46 (56%)
Frame = +3
Query: 3 PPLRIRDLPTFLTITDMEHPMALILALLKEDLDVLDREASTARVLA 140
PPLR+RDLP+F+ T + P A + +E + LD + VLA
Sbjct: 195 PPLRVRDLPSFIVSTSEDDPYAFVADAFRELVGRLDGGEDSPSVLA 240
>gb|ACM66950.1| flavonoid glucosyltransferase [Crocus sativus]
Length = 497
Score = 49.7 bits (117), Expect(2) = 1e-10
Identities = 25/51 (49%), Positives = 32/51 (62%), Gaps = 4/51 (7%)
Frame = +1
Query: 244 IGPVMSVNGP----NLFELDEQRYMEWLDSKAEGSVVYVSFGSFTTFKKRQ 384
+GP++ G +L + DE+ YMEWLDSK E SVVYVS S KK+Q
Sbjct: 246 VGPILPCKGGVSRGDLLKEDEKGYMEWLDSKPENSVVYVSLESLAVLKKQQ 296
Score = 41.6 bits (96), Expect(2) = 1e-10
Identities = 18/44 (40%), Positives = 33/44 (75%)
Frame = +3
Query: 6 PLRIRDLPTFLTITDMEHPMALILALLKEDLDVLDREASTARVL 137
P+++RDLP+FLTI + P A++L+++++ + LDRE + +VL
Sbjct: 181 PVQVRDLPSFLTIKP-DDPYAVVLSMIRDSFEGLDREETKTKVL 223
>ref|XP_004965984.1| PREDICTED: UDP-glycosyltransferase 75D1-like [Setaria italica]
Length = 473
Score = 51.6 bits (122), Expect(2) = 1e-09
Identities = 24/54 (44%), Positives = 35/54 (64%), Gaps = 1/54 (1%)
Frame = +1
Query: 238 MAIGPVMSVNGP-NLFELDEQRYMEWLDSKAEGSVVYVSFGSFTTFKKRQAEEI 396
+ IGPV+ ++ + D+ +YMEWLD+K SVVYVSFGS T + Q +E+
Sbjct: 252 LPIGPVLPTGEEASILKPDDAKYMEWLDTKPASSVVYVSFGSLATMAREQLDEL 305
Score = 36.6 bits (83), Expect(2) = 1e-09
Identities = 19/45 (42%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
Frame = +3
Query: 6 PLRIRDLPTFLT-ITDMEHPMALILALLKEDLDVLDREASTARVL 137
PL +RDLPTFLT TD H + ++ D LD+E A +L
Sbjct: 187 PLAVRDLPTFLTESTDPAHYFHAVFLTFRDLFDTLDKETCKATIL 231
>ref|NP_001057977.1| Os06g0593800 [Oryza sativa Japonica Group]
gi|50725398|dbj|BAD32872.1| putative anthocyanin
5-O-glucosyltransferase [Oryza sativa Japonica Group]
gi|50725648|dbj|BAD33114.1| putative anthocyanin
5-O-glucosyltransferase [Oryza sativa Japonica Group]
gi|113596017|dbj|BAF19891.1| Os06g0593800 [Oryza sativa
Japonica Group]
Length = 469
Score = 55.5 bits (132), Expect(2) = 2e-09
Identities = 27/54 (50%), Positives = 38/54 (70%), Gaps = 1/54 (1%)
Frame = +1
Query: 238 MAIGPVM-SVNGPNLFELDEQRYMEWLDSKAEGSVVYVSFGSFTTFKKRQAEEI 396
+ IGPV+ S + LF+ ++ +YMEWLD+K GSVVYV+FGS T K Q +E+
Sbjct: 246 LPIGPVLPSGDDAALFKQNDAKYMEWLDTKPAGSVVYVAFGSLTVMAKGQVDEL 299
Score = 32.0 bits (71), Expect(2) = 2e-09
Identities = 19/46 (41%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = +3
Query: 3 PPLRIRDLPTFLT-ITDMEHPMALILALLKEDLDVLDREASTARVL 137
PP+ DLP+FLT TD I ++ D LDRE A VL
Sbjct: 180 PPMAAGDLPSFLTEATDPSDYFHSIFTTFRDLFDALDRETPKATVL 225
>gb|EEE65963.1| hypothetical protein OsJ_21854 [Oryza sativa Japonica Group]
Length = 450
Score = 55.5 bits (132), Expect(2) = 2e-09
Identities = 27/54 (50%), Positives = 38/54 (70%), Gaps = 1/54 (1%)
Frame = +1
Query: 238 MAIGPVM-SVNGPNLFELDEQRYMEWLDSKAEGSVVYVSFGSFTTFKKRQAEEI 396
+ IGPV+ S + LF+ ++ +YMEWLD+K GSVVYV+FGS T K Q +E+
Sbjct: 227 LPIGPVLPSGDDAALFKQNDAKYMEWLDTKPAGSVVYVAFGSLTVMAKGQVDEL 280
Score = 32.0 bits (71), Expect(2) = 2e-09
Identities = 19/46 (41%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = +3
Query: 3 PPLRIRDLPTFLT-ITDMEHPMALILALLKEDLDVLDREASTARVL 137
PP+ DLP+FLT TD I ++ D LDRE A VL
Sbjct: 161 PPMAAGDLPSFLTEATDPSDYFHSIFTTFRDLFDALDRETPKATVL 206
>ref|XP_002438626.1| hypothetical protein SORBIDRAFT_10g023070 [Sorghum bicolor]
gi|241916849|gb|EER89993.1| hypothetical protein
SORBIDRAFT_10g023070 [Sorghum bicolor]
Length = 492
Score = 50.8 bits (120), Expect(2) = 3e-09
Identities = 28/56 (50%), Positives = 34/56 (60%), Gaps = 3/56 (5%)
Frame = +1
Query: 238 MAIGPVMSVNGP--NLF-ELDEQRYMEWLDSKAEGSVVYVSFGSFTTFKKRQAEEI 396
+ IGPV+ ++F E D RYMEWL SK SVVYVSFGS T + Q EE+
Sbjct: 267 LPIGPVLPTGDEETSMFKEEDAARYMEWLHSKPPNSVVYVSFGSLATMAREQVEEL 322
Score = 35.8 bits (81), Expect(2) = 3e-09
Identities = 22/48 (45%), Positives = 27/48 (56%), Gaps = 3/48 (6%)
Frame = +3
Query: 3 PPLRIRDLPTFLT-ITDMEHPMALILALLKEDLDVLDREA--STARVL 137
PPL +RDLPTFLT TD + ++ D LDRE STA +L
Sbjct: 197 PPLAVRDLPTFLTESTDPGDYFHTVFLTFRDLFDTLDRETSNSTATIL 244
>ref|XP_006657100.1| PREDICTED: UDP-glycosyltransferase 75D1-like [Oryza brachyantha]
Length = 353
Score = 56.6 bits (135), Expect(2) = 5e-09
Identities = 28/52 (53%), Positives = 37/52 (71%), Gaps = 1/52 (1%)
Frame = +1
Query: 244 IGPVM-SVNGPNLFELDEQRYMEWLDSKAEGSVVYVSFGSFTTFKKRQAEEI 396
IGPV+ S + LF+ D+ +YMEWLD+K GSVVYV+FGS T K Q +E+
Sbjct: 244 IGPVLPSGDEAALFKQDDSKYMEWLDTKPAGSVVYVAFGSLTVMAKGQLDEL 295
Score = 29.3 bits (64), Expect(2) = 5e-09
Identities = 18/46 (39%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = +3
Query: 3 PPLRIRDLPTFL-TITDMEHPMALILALLKEDLDVLDREASTARVL 137
PPL + DLP+FL +D I ++ D LDRE A VL
Sbjct: 176 PPLPVGDLPSFLIEASDPSDFFHSIFTTFRDLFDTLDREIPKATVL 221
>ref|XP_002263532.1| PREDICTED: UDP-glycosyltransferase 75C1-like [Vitis vinifera]
Length = 447
Score = 55.5 bits (132), Expect(2) = 2e-07
Identities = 29/56 (51%), Positives = 39/56 (69%), Gaps = 3/56 (5%)
Frame = +1
Query: 238 MAIGPVMSVNGP---NLFELDEQRYMEWLDSKAEGSVVYVSFGSFTTFKKRQAEEI 396
+AIGP+M ++ +LFE + Y+ WL+SK EGSV+YVSFGS T +K Q EEI
Sbjct: 227 VAIGPLMQLDSSISCDLFERSKD-YLPWLNSKPEGSVIYVSFGSLATLQKNQMEEI 281
Score = 25.4 bits (54), Expect(2) = 2e-07
Identities = 13/45 (28%), Positives = 25/45 (55%)
Frame = +3
Query: 3 PPLRIRDLPTFLTITDMEHPMALILALLKEDLDVLDREASTARVL 137
PPL+ DLP+ L T P A ++ +E + L+++ +T ++
Sbjct: 165 PPLKYEDLPSILLPTS---PHASVVPSFQEHVQNLEQDPNTCLLI 206
>gb|ACD03260.1| UDP-glycosyltransferase UGT75E2 [Avena strigosa]
Length = 507
Score = 53.1 bits (126), Expect(2) = 3e-07
Identities = 28/58 (48%), Positives = 37/58 (63%), Gaps = 6/58 (10%)
Frame = +1
Query: 241 AIGPVMSVNGPNLFEL------DEQRYMEWLDSKAEGSVVYVSFGSFTTFKKRQAEEI 396
A+GPVM + L D++RYMEWL ++ E SVVY+SFGS T+ K+Q EEI
Sbjct: 267 AVGPVMGSSAVARIHLFHHAGADKKRYMEWLGAQPEISVVYISFGSVWTYSKQQMEEI 324
Score = 26.6 bits (57), Expect(2) = 3e-07
Identities = 14/44 (31%), Positives = 21/44 (47%)
Frame = +3
Query: 6 PLRIRDLPTFLTITDMEHPMALILALLKEDLDVLDREASTARVL 137
PLR RD P+FL T + L +E + +D + A+ L
Sbjct: 201 PLRTRDFPSFLVDTTGGEVAKSVNDLFRELFEFMDAQGQRAKFL 244
>ref|XP_003566479.1| PREDICTED: UDP-glycosyltransferase 75D1-like [Brachypodium
distachyon]
Length = 784
Score = 40.8 bits (94), Expect(2) = 4e-07
Identities = 27/65 (41%), Positives = 35/65 (53%), Gaps = 12/65 (18%)
Frame = +1
Query: 238 MAIGPVMSV---------NGP--NLFELD-EQRYMEWLDSKAEGSVVYVSFGSFTTFKKR 381
+ IGPV+S N P +LF D E Y+ WLD++ SVVY+SFGS + K
Sbjct: 277 VTIGPVLSFLHDEADGNNNSPPNDLFGHDGEGGYLSWLDAQRAKSVVYISFGSTSVMSKA 336
Query: 382 QAEEI 396
Q EI
Sbjct: 337 QVAEI 341
Score = 38.5 bits (88), Expect(2) = 4e-07
Identities = 17/43 (39%), Positives = 26/43 (60%)
Frame = +3
Query: 3 PPLRIRDLPTFLTITDMEHPMALILALLKEDLDVLDREASTAR 131
PPLR+RDLP+FL IT + A ++ +D ++R+ AR
Sbjct: 206 PPLRLRDLPSFLAITSDDDRFAAVIPEFAALIDAIERDGDPAR 248
>ref|XP_003560622.1| PREDICTED: UDP-glycosyltransferase 75B1-like [Brachypodium
distachyon]
Length = 478
Score = 49.7 bits (117), Expect(2) = 4e-07
Identities = 27/61 (44%), Positives = 38/61 (62%), Gaps = 9/61 (14%)
Frame = +1
Query: 238 MAIGPVM--------SVNGPNLFELDEQR-YMEWLDSKAEGSVVYVSFGSFTTFKKRQAE 390
+A+GPV+ + + +LF D+ + YMEWLD+K SVVYVSFG+ + KRQ E
Sbjct: 255 VAVGPVVPDPDEASTAASSTDLFPRDDGKAYMEWLDTKPARSVVYVSFGTIVSMSKRQEE 314
Query: 391 E 393
E
Sbjct: 315 E 315
Score = 29.6 bits (65), Expect(2) = 4e-07
Identities = 16/44 (36%), Positives = 24/44 (54%)
Frame = +3
Query: 3 PPLRIRDLPTFLTITDMEHPMALILALLKEDLDVLDREASTARV 134
P L+ LP+ ++IT EHP L+L +++E LD S V
Sbjct: 192 PRLKSSALPSVVSITSPEHPHYLLLDMMRELFLTLDEYKSKVLV 235
>dbj|BAJ96117.1| predicted protein [Hordeum vulgare subsp. vulgare]
Length = 497
Score = 58.5 bits (140), Expect = 9e-07
Identities = 27/41 (65%), Positives = 32/41 (78%)
Frame = +1
Query: 274 NLFELDEQRYMEWLDSKAEGSVVYVSFGSFTTFKKRQAEEI 396
+LFE DE+ YMEWLDSK GSVVYVSFGS + KRQ +E+
Sbjct: 295 DLFEHDEKGYMEWLDSKPAGSVVYVSFGSMSAVSKRQKDEL 335
>sp|Q767C8.1|IH5GT_IRIHO RecName: Full=Cyanidin 3-O-rutinoside 5-O-glucosyltransferase;
Short=Ih5GT; AltName: Full=Anthocyanin
5-O-glucosyltransferase; AltName:
Full=Cyanidin-3-rhamnosylglucoside
5-O-glucosyltransferase; AltName: Full=Uridine
diphosphoglucose-cyanidin 3-rhamnosylglucoside
5-O-glucosyltransferase gi|40714353|dbj|BAD06874.1|
anthocyanin 5-O-glucosyltransferase [Iris hollandica]
Length = 463
Score = 58.2 bits (139), Expect = 1e-06
Identities = 25/41 (60%), Positives = 31/41 (75%)
Frame = +1
Query: 274 NLFELDEQRYMEWLDSKAEGSVVYVSFGSFTTFKKRQAEEI 396
+L+E D YMEWLD K EGSVVYVSFGS+ T K+ + EE+
Sbjct: 269 DLYEHDTSNYMEWLDGKEEGSVVYVSFGSYATLKEEEREEV 309
>ref|XP_003560621.1| PREDICTED: UDP-glycosyltransferase 75D1-like [Brachypodium
distachyon]
Length = 489
Score = 48.1 bits (113), Expect(2) = 1e-06
Identities = 24/58 (41%), Positives = 36/58 (62%), Gaps = 5/58 (8%)
Frame = +1
Query: 238 MAIGPVMSVNGPN----LFELDEQ-RYMEWLDSKAEGSVVYVSFGSFTTFKKRQAEEI 396
+ +GP++ + + LF+ DE RYMEWLD K SVVYV+FGS ++ Q +E+
Sbjct: 254 LPVGPLLPTSSGDDEAGLFKQDEDARYMEWLDGKPANSVVYVAFGSLARMEREQLDEL 311
Score = 29.6 bits (65), Expect(2) = 1e-06
Identities = 20/49 (40%), Positives = 28/49 (57%), Gaps = 4/49 (8%)
Frame = +3
Query: 3 PPLRIRDLPTFLT-ITDMEHPMALILALLKE--DLDVL-DREASTARVL 137
PPL +RDLP+FLT TD + +++ D D L D++A A VL
Sbjct: 184 PPLALRDLPSFLTESTDPSDQFHSVYTAIRDLFDFDPLDDKDAPKATVL 232
>dbj|BAJ90555.1| predicted protein [Hordeum vulgare subsp. vulgare]
Length = 511
Score = 50.1 bits (118), Expect(2) = 2e-06
Identities = 26/58 (44%), Positives = 36/58 (62%), Gaps = 6/58 (10%)
Frame = +1
Query: 241 AIGPVMSVNGPNLFEL------DEQRYMEWLDSKAEGSVVYVSFGSFTTFKKRQAEEI 396
A+GP++ + L D+ RYMEWL ++ E SVVYVSFGS T+ ++Q EEI
Sbjct: 264 AVGPMVGSSAEARIHLFNHAGADKTRYMEWLGAQPERSVVYVSFGSIWTYSEQQMEEI 321
Score = 27.3 bits (59), Expect(2) = 2e-06
Identities = 14/44 (31%), Positives = 22/44 (50%)
Frame = +3
Query: 6 PLRIRDLPTFLTITDMEHPMALILALLKEDLDVLDREASTARVL 137
P+RIRD P+FL T ++ A E + +D + +VL
Sbjct: 198 PIRIRDFPSFLVDTTGAEVGKVVNAAFCELFEFMDEQTRDVKVL 241
>ref|XP_006664328.1| PREDICTED: cyanidin 3-O-rutinoside 5-O-glucosyltransferase-like
[Oryza brachyantha]
Length = 499
Score = 56.6 bits (135), Expect = 3e-06
Identities = 26/49 (53%), Positives = 34/49 (69%)
Frame = +1
Query: 250 PVMSVNGPNLFELDEQRYMEWLDSKAEGSVVYVSFGSFTTFKKRQAEEI 396
PV + ++F+ D + YMEWLD+ E SVVY+SFGS T+ KRQ EEI
Sbjct: 273 PVSTKARIHMFQQDSKNYMEWLDTHGERSVVYISFGSVLTYSKRQIEEI 321
>emb|CBX25382.1| hypothetical_protein [Oryza brachyantha]
Length = 496
Score = 56.6 bits (135), Expect = 3e-06
Identities = 26/49 (53%), Positives = 34/49 (69%)
Frame = +1
Query: 250 PVMSVNGPNLFELDEQRYMEWLDSKAEGSVVYVSFGSFTTFKKRQAEEI 396
PV + ++F+ D + YMEWLD+ E SVVY+SFGS T+ KRQ EEI
Sbjct: 270 PVSTKARIHMFQQDSKNYMEWLDTHGERSVVYISFGSVLTYSKRQIEEI 318
>ref|XP_003577063.1| PREDICTED: UDP-glycosyltransferase 75D1-like [Brachypodium
distachyon]
Length = 543
Score = 46.6 bits (109), Expect(2) = 4e-06
Identities = 25/60 (41%), Positives = 36/60 (60%), Gaps = 9/60 (15%)
Frame = +1
Query: 244 IGPVMSVNGPN--------LFELDEQ-RYMEWLDSKAEGSVVYVSFGSFTTFKKRQAEEI 396
+GPV++ N LF+ D + +Y+ WLD++ SV+YVSFGS T+ K Q EEI
Sbjct: 273 VGPVVASGSSNNNASRNIHLFDHDNKAQYISWLDAQPASSVIYVSFGSIWTYSKPQMEEI 332
Score = 29.6 bits (65), Expect(2) = 4e-06
Identities = 17/45 (37%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
Frame = +3
Query: 6 PLRIRDLPTFLTITDMEHPMALILALLKEDLDVLDREA-STARVL 137
PLRIRD PTFL T + + L+E + +D++ + A+VL
Sbjct: 205 PLRIRDFPTFLVDTTGSDIASSVNEALRELFEFMDQQGKNNAKVL 249
>gb|EMT24761.1| Indole-3-acetate beta-glucosyltransferase 1 [Aegilops tauschii]
Length = 216
Score = 56.2 bits (134), Expect = 4e-06
Identities = 29/63 (46%), Positives = 39/63 (61%), Gaps = 12/63 (19%)
Frame = +1
Query: 244 IGPVMSV------------NGPNLFELDEQRYMEWLDSKAEGSVVYVSFGSFTTFKKRQA 387
IGPV+S N +LF+LD + Y+EWLD++A GSVVY+SFGS + KRQ
Sbjct: 42 IGPVLSFLDASAASASNNNNNNDLFKLDGKGYLEWLDAQAAGSVVYISFGSSSAMSKRQI 101
Query: 388 EEI 396
E+
Sbjct: 102 TEV 104
>ref|NP_001065729.1| Os11g0145200 [Oryza sativa Japonica Group]
gi|77548647|gb|ABA91444.1| Indole-3-acetate
beta-glucosyltransferase, putative, expressed [Oryza
sativa Japonica Group] gi|113644433|dbj|BAF27574.1|
Os11g0145200 [Oryza sativa Japonica Group]
gi|125576199|gb|EAZ17421.1| hypothetical protein
OsJ_32945 [Oryza sativa Japonica Group]
gi|215765889|dbj|BAG98117.1| unnamed protein product
[Oryza sativa Japonica Group]
Length = 482
Score = 56.2 bits (134), Expect = 4e-06
Identities = 23/41 (56%), Positives = 33/41 (80%)
Frame = +1
Query: 274 NLFELDEQRYMEWLDSKAEGSVVYVSFGSFTTFKKRQAEEI 396
++F+ D++ YMEWLD+ +E SVVY+SFGS T+ KRQ +EI
Sbjct: 274 HMFQRDKKNYMEWLDTHSERSVVYISFGSILTYSKRQVDEI 314