BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Zingiber23_contig00006294
         (462 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AHF20170.1| 4-coumarate:ligase, partial [Musa acuminata AAA G...   200   2e-49
ref|XP_006474168.1| PREDICTED: 4-coumarate--CoA ligase 2-like [C...   183   2e-44
ref|XP_006453393.1| hypothetical protein CICLE_v10007733mg [Citr...   183   2e-44
gb|AGW24660.1| 4-coumarate:coenzyme A ligase [Galtonia saundersiae]   182   4e-44
gb|EOY31810.1| 4-coumarate:CoA ligase 2 [Theobroma cacao]             181   9e-44
ref|XP_002324477.2| 4-coumarate-CoA ligase 2 family protein [Pop...   181   1e-43
gb|AAC39366.1| 4-coumarate:CoA ligase 1 [Populus trichocarpa x P...   180   2e-43
gb|AHF20171.1| 4-coumarate:ligase, partial [Musa acuminata AAA G...   179   3e-43
gb|AAC39365.1| 4-coumarate:CoA ligase 2 [Populus trichocarpa x P...   179   3e-43
gb|AFU90743.1| 4-coumarate:coenzyme A ligase [Punica granatum]        179   4e-43
gb|ADE96996.1| p-coumarate:CoA-ligase 2 [Sorbus aucuparia]            178   6e-43
gb|ADF30254.1| p-coumarate:CoA ligase 1 [Sorbus aucuparia]            178   8e-43
ref|XP_006381817.1| hypothetical protein POPTR_0006s18490g [Popu...   177   1e-42
ref|XP_004309949.1| PREDICTED: 4-coumarate--CoA ligase 1-like [F...   177   1e-42
gb|AFY97682.1| 4-coumarate:coenzyme A ligase 2 [Pyrus pyrifolia]      177   1e-42
ref|XP_002329649.1| 4-Coumarate:CoA ligase [Populus trichocarpa]      177   1e-42
gb|EXC37569.1| 4-coumarate--CoA ligase 1 [Morus notabilis]            177   1e-42
gb|AFY97681.1| 4-coumarate:coenzyme A ligase 1 [Pyrus pyrifolia]      177   1e-42
gb|AFC89541.1| 4-coumarate: coenzyme A ligase 5 [Populus tomentosa]   177   1e-42
gb|AAF91309.1|AF239686_1 4-coumarate:coA ligase 2 [Rubus idaeus]      177   2e-42

>gb|AHF20170.1| 4-coumarate:ligase, partial [Musa acuminata AAA Group]
          Length = 298

 Score =  200 bits (508), Expect = 2e-49
 Identities = 98/110 (89%), Positives = 107/110 (97%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KEIIK+KGFQVAPAELEALL+ HPNI+DAAVVPMKDEAAGEVPVAFVVRSNGSKI++DEI
Sbjct: 189 KEIIKYKGFQVAPAELEALLIAHPNIADAAVVPMKDEAAGEVPVAFVVRSNGSKISEDEI 248

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLAEQFPIGPFP 332
           KQYIS++VVFYKRINKVFFTE IPK+PSGKILRKDLRAKLA +FPIGPFP
Sbjct: 249 KQYISKEVVFYKRINKVFFTEAIPKSPSGKILRKDLRAKLAARFPIGPFP 298


>ref|XP_006474168.1| PREDICTED: 4-coumarate--CoA ligase 2-like [Citrus sinensis]
          Length = 544

 Score =  183 bits (465), Expect = 2e-44
 Identities = 91/101 (90%), Positives = 98/101 (97%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IKFKGFQVAPAELEA+L+ HPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKIT+DEI
Sbjct: 438 KELIKFKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITEDEI 497

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLA 305
           KQYIS+QVVFYKRIN+VFFT+ IPKAPSGKILRKDLR KLA
Sbjct: 498 KQYISKQVVFYKRINRVFFTDSIPKAPSGKILRKDLRTKLA 538


>ref|XP_006453393.1| hypothetical protein CICLE_v10007733mg [Citrus clementina]
           gi|557556619|gb|ESR66633.1| hypothetical protein
           CICLE_v10007733mg [Citrus clementina]
          Length = 635

 Score =  183 bits (465), Expect = 2e-44
 Identities = 91/101 (90%), Positives = 98/101 (97%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IKFKGFQVAPAELEA+L+ HPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKIT+DEI
Sbjct: 529 KELIKFKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITEDEI 588

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLA 305
           KQYIS+QVVFYKRIN+VFFT+ IPKAPSGKILRKDLR KLA
Sbjct: 589 KQYISKQVVFYKRINRVFFTDSIPKAPSGKILRKDLRTKLA 629


>gb|AGW24660.1| 4-coumarate:coenzyme A ligase [Galtonia saundersiae]
          Length = 545

 Score =  182 bits (462), Expect = 4e-44
 Identities = 89/108 (82%), Positives = 101/108 (93%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK+KGFQVAPAELEA+L+ HPNI+DAAVVPMKDE AGE+PVAFV+RSNGS+IT+DEI
Sbjct: 436 KELIKYKGFQVAPAELEAMLITHPNIADAAVVPMKDELAGELPVAFVLRSNGSEITEDEI 495

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLAEQFPIGP 326
           KQYIS+QVVFYKRI++VFF E IPKAPSGKILRKDLRAKLA  FP GP
Sbjct: 496 KQYISKQVVFYKRIHRVFFIEAIPKAPSGKILRKDLRAKLAAGFPNGP 543


>gb|EOY31810.1| 4-coumarate:CoA ligase 2 [Theobroma cacao]
          Length = 542

 Score =  181 bits (459), Expect = 9e-44
 Identities = 89/105 (84%), Positives = 99/105 (94%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK+KGFQVAPAELEA+L+ HPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKIT+DEI
Sbjct: 437 KELIKYKGFQVAPAELEAMLIGHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITEDEI 496

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLAEQFP 317
           KQ+IS+QVVFYKR+ +VFFTE IPKAPSGKILRKDLRA+LA   P
Sbjct: 497 KQFISKQVVFYKRLGQVFFTEAIPKAPSGKILRKDLRARLAADIP 541


>ref|XP_002324477.2| 4-coumarate-CoA ligase 2 family protein [Populus trichocarpa]
           gi|550318444|gb|EEF03042.2| 4-coumarate-CoA ligase 2
           family protein [Populus trichocarpa]
          Length = 556

 Score =  181 bits (458), Expect = 1e-43
 Identities = 88/101 (87%), Positives = 98/101 (97%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK+KGFQVAPAELEA+L+ HPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKIT+DEI
Sbjct: 438 KELIKYKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITEDEI 497

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLA 305
           KQYIS+QV+FYKRI +VFFTE IPKAPSGKILRKDLRA++A
Sbjct: 498 KQYISKQVIFYKRIGRVFFTEAIPKAPSGKILRKDLRARVA 538


>gb|AAC39366.1| 4-coumarate:CoA ligase 1 [Populus trichocarpa x Populus deltoides]
          Length = 557

 Score =  180 bits (457), Expect = 2e-43
 Identities = 90/119 (75%), Positives = 105/119 (88%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK+KGFQVAPAELEA+L+ HP+ISD AVVPMKDEAAGEVP+AFVVR+NGSKIT+DEI
Sbjct: 439 KELIKYKGFQVAPAELEAMLIAHPDISDCAVVPMKDEAAGEVPIAFVVRANGSKITEDEI 498

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLAEQFPIGPFP*SYRHGQYL 359
           KQYIS+QVVFYKRI++VFFTE IPKAPSGKILRKDLRA+LA     G F   ++H  Y+
Sbjct: 499 KQYISKQVVFYKRISRVFFTEAIPKAPSGKILRKDLRARLA----TGDFLIKFQHDTYM 553


>gb|AHF20171.1| 4-coumarate:ligase, partial [Musa acuminata AAA Group]
          Length = 381

 Score =  179 bits (455), Expect = 3e-43
 Identities = 89/104 (85%), Positives = 100/104 (96%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK KGFQVAPAELEALL+ HP+I+DAAVVPMKDEAAGEVPVAFVVRSNGS+I++DEI
Sbjct: 276 KELIKSKGFQVAPAELEALLIAHPSIADAAVVPMKDEAAGEVPVAFVVRSNGSEISEDEI 335

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLAEQF 314
           KQ++S+QVVFYKRINKVFFTE IPKAPSGKILRKDLRAKLA +F
Sbjct: 336 KQHVSKQVVFYKRINKVFFTEAIPKAPSGKILRKDLRAKLAARF 379


>gb|AAC39365.1| 4-coumarate:CoA ligase 2 [Populus trichocarpa x Populus deltoides]
          Length = 548

 Score =  179 bits (455), Expect = 3e-43
 Identities = 87/101 (86%), Positives = 98/101 (97%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK+KGFQVAPAELEA+L+ HPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKIT+DEI
Sbjct: 438 KELIKYKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITEDEI 497

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLA 305
           KQYIS+QV+FYKRI +VFFTE IPKAPSGKILRKDLRA+++
Sbjct: 498 KQYISKQVIFYKRIGRVFFTEAIPKAPSGKILRKDLRARVS 538


>gb|AFU90743.1| 4-coumarate:coenzyme A ligase [Punica granatum]
          Length = 544

 Score =  179 bits (453), Expect = 4e-43
 Identities = 88/101 (87%), Positives = 98/101 (97%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK+KGFQVAPAELEA+L+ HP+ISDAAVVPMKDEAAGEVPVAFV+RSNGSKIT+D+I
Sbjct: 439 KELIKYKGFQVAPAELEAMLIAHPSISDAAVVPMKDEAAGEVPVAFVIRSNGSKITEDDI 498

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLA 305
           KQYIS+QVVFYKRIN+VFFTE IPKAPSGKILRK LRAKLA
Sbjct: 499 KQYISKQVVFYKRINRVFFTEAIPKAPSGKILRKYLRAKLA 539


>gb|ADE96996.1| p-coumarate:CoA-ligase 2 [Sorbus aucuparia]
          Length = 547

 Score =  178 bits (452), Expect = 6e-43
 Identities = 87/105 (82%), Positives = 98/105 (93%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK+KGFQVAPAELEA+L+ HPNISDAAVVPMKDEAAGE+PVAFVVRSNGSKI++D+I
Sbjct: 442 KELIKYKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEIPVAFVVRSNGSKISEDDI 501

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLAEQFP 317
           KQYIS+QVVFY RI +VFFT+ IPKAPSGKILRKDLRAKLA   P
Sbjct: 502 KQYISKQVVFYMRIGRVFFTDKIPKAPSGKILRKDLRAKLAASLP 546


>gb|ADF30254.1| p-coumarate:CoA ligase 1 [Sorbus aucuparia]
          Length = 547

 Score =  178 bits (451), Expect = 8e-43
 Identities = 86/105 (81%), Positives = 99/105 (94%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK+KGFQVAPAELEALL+ HP++SDAAVVPMKDEAAGEVPVAFVVRSN S++T+DE+
Sbjct: 442 KELIKYKGFQVAPAELEALLITHPSVSDAAVVPMKDEAAGEVPVAFVVRSNNSQLTEDEV 501

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLAEQFP 317
           KQ+IS+QVVFYKRIN+VFF E IPK+PSGKILRKDLRAKLA  FP
Sbjct: 502 KQFISKQVVFYKRINRVFFIEAIPKSPSGKILRKDLRAKLAAGFP 546


>ref|XP_006381817.1| hypothetical protein POPTR_0006s18490g [Populus trichocarpa]
           gi|550336573|gb|ERP59614.1| hypothetical protein
           POPTR_0006s18490g [Populus trichocarpa]
          Length = 543

 Score =  177 bits (450), Expect = 1e-42
 Identities = 86/101 (85%), Positives = 98/101 (97%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK+KGFQVAPAELEA+L+ HP+ISD AVVPMKDEAAGEVP+AFVVR+NGSKIT+DEI
Sbjct: 437 KELIKYKGFQVAPAELEAMLIAHPDISDCAVVPMKDEAAGEVPIAFVVRANGSKITEDEI 496

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLA 305
           KQYIS+QVVFYKRI++VFFTE IPKAPSGKILRKDLRA+LA
Sbjct: 497 KQYISKQVVFYKRISRVFFTEAIPKAPSGKILRKDLRARLA 537


>ref|XP_004309949.1| PREDICTED: 4-coumarate--CoA ligase 1-like [Fragaria vesca subsp.
           vesca]
          Length = 545

 Score =  177 bits (450), Expect = 1e-42
 Identities = 85/105 (80%), Positives = 100/105 (95%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK+KGFQVAPAELEA+L+ HPN+SDAAVVPMKDEAAGEVP+AFVVRSNGSKI++D+I
Sbjct: 440 KELIKYKGFQVAPAELEAMLISHPNLSDAAVVPMKDEAAGEVPIAFVVRSNGSKISEDDI 499

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLAEQFP 317
           KQY+S+QVVFYKRI++VFFT+ IPKAPSGKILRKDLRA+LA   P
Sbjct: 500 KQYVSKQVVFYKRISRVFFTDKIPKAPSGKILRKDLRARLAAGVP 544


>gb|AFY97682.1| 4-coumarate:coenzyme A ligase 2 [Pyrus pyrifolia]
          Length = 547

 Score =  177 bits (450), Expect = 1e-42
 Identities = 87/105 (82%), Positives = 98/105 (93%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK+KGFQVAPAELEA+L+ HPNISDAAVVPMKDEAAGE+PVAFVVRSNGSKI++D+I
Sbjct: 442 KELIKYKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEIPVAFVVRSNGSKISEDDI 501

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLAEQFP 317
           KQYIS+QVVFYKRI +VFF + IPKAPSGKILRKDLRAKLA   P
Sbjct: 502 KQYISKQVVFYKRIGRVFFIDKIPKAPSGKILRKDLRAKLAAGLP 546


>ref|XP_002329649.1| 4-Coumarate:CoA ligase [Populus trichocarpa]
          Length = 545

 Score =  177 bits (450), Expect = 1e-42
 Identities = 86/101 (85%), Positives = 98/101 (97%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK+KGFQVAPAELEA+L+ HP+ISD AVVPMKDEAAGEVP+AFVVR+NGSKIT+DEI
Sbjct: 439 KELIKYKGFQVAPAELEAMLIAHPDISDCAVVPMKDEAAGEVPIAFVVRANGSKITEDEI 498

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLA 305
           KQYIS+QVVFYKRI++VFFTE IPKAPSGKILRKDLRA+LA
Sbjct: 499 KQYISKQVVFYKRISRVFFTEAIPKAPSGKILRKDLRARLA 539


>gb|EXC37569.1| 4-coumarate--CoA ligase 1 [Morus notabilis]
          Length = 546

 Score =  177 bits (449), Expect = 1e-42
 Identities = 87/105 (82%), Positives = 99/105 (94%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK+KGFQVAPAELEALL+ HP+ISDAAVVPMKDEAAGEVPVAFVVR+NGS+IT+DEI
Sbjct: 441 KELIKYKGFQVAPAELEALLLTHPHISDAAVVPMKDEAAGEVPVAFVVRANGSQITEDEI 500

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLAEQFP 317
           KQ++S+QVVFYKRIN+V F E IPK+PSGKILRKDLRAKLA  FP
Sbjct: 501 KQFVSKQVVFYKRINRVIFIEAIPKSPSGKILRKDLRAKLAAGFP 545


>gb|AFY97681.1| 4-coumarate:coenzyme A ligase 1 [Pyrus pyrifolia]
          Length = 547

 Score =  177 bits (449), Expect = 1e-42
 Identities = 86/105 (81%), Positives = 99/105 (94%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK+KGFQVAPAELEALL+ HP++SDAAVVPMKDEAAGEVPVAFVVRSN S++T+DE+
Sbjct: 442 KELIKYKGFQVAPAELEALLITHPSVSDAAVVPMKDEAAGEVPVAFVVRSNKSQLTEDEV 501

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLAEQFP 317
           KQ+IS+QVVFYKRIN+VFF E IPK+PSGKILRKDLRAKLA  FP
Sbjct: 502 KQFISKQVVFYKRINRVFFIEAIPKSPSGKILRKDLRAKLAAGFP 546


>gb|AFC89541.1| 4-coumarate: coenzyme A ligase 5 [Populus tomentosa]
          Length = 545

 Score =  177 bits (449), Expect = 1e-42
 Identities = 87/101 (86%), Positives = 98/101 (97%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK+KGFQVAPAELEA+L+ HP+ISD AVVPMKDEAAGEVPVAFVVR+NGSKIT+DEI
Sbjct: 439 KELIKYKGFQVAPAELEAMLIAHPDISDCAVVPMKDEAAGEVPVAFVVRANGSKITEDEI 498

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLA 305
           KQYIS+QVVFYKRI++VFFTE IPKAPSGKILRKDLRA+LA
Sbjct: 499 KQYISKQVVFYKRISRVFFTESIPKAPSGKILRKDLRARLA 539


>gb|AAF91309.1|AF239686_1 4-coumarate:coA ligase 2 [Rubus idaeus]
          Length = 544

 Score =  177 bits (448), Expect = 2e-42
 Identities = 87/105 (82%), Positives = 99/105 (94%)
 Frame = +3

Query: 3   KEIIKFKGFQVAPAELEALLVMHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITQDEI 182
           KE+IK+KGFQVAPAELEA+L+ HPN+SDAAVV MKDEAAGEVPVAFVVRSNGSKI++D+I
Sbjct: 439 KELIKYKGFQVAPAELEAMLISHPNLSDAAVVSMKDEAAGEVPVAFVVRSNGSKISEDDI 498

Query: 183 KQYISRQVVFYKRINKVFFTEVIPKAPSGKILRKDLRAKLAEQFP 317
           KQYIS+QVVFYKRI+KVFFT+ IPKAPSGKILRKDLRA+LA   P
Sbjct: 499 KQYISKQVVFYKRISKVFFTDKIPKAPSGKILRKDLRARLAAGLP 543