BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Zanthoxylum22_contig00019397
         (380 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|KDO58932.1| hypothetical protein CISIN_1g0268681mg [Citrus si...   181   2e-43
ref|XP_006482300.1| PREDICTED: probable transmembrane ascorbate ...   181   2e-43
ref|XP_006430824.1| hypothetical protein CICLE_v10012646mg [Citr...   181   2e-43
ref|XP_006430823.1| hypothetical protein CICLE_v10012646mg [Citr...   181   2e-43
ref|XP_007151758.1| hypothetical protein PHAVU_004G072300g [Phas...   169   6e-40
ref|XP_012471058.1| PREDICTED: probable transmembrane ascorbate ...   166   5e-39
gb|KJB06294.1| hypothetical protein B456_001G081800 [Gossypium r...   166   5e-39
gb|KJB06293.1| hypothetical protein B456_001G081800 [Gossypium r...   166   5e-39
emb|CDP02400.1| unnamed protein product [Coffea canephora]            166   6e-39
ref|XP_010692475.1| PREDICTED: probable transmembrane ascorbate ...   165   1e-38
ref|XP_010692471.1| PREDICTED: probable transmembrane ascorbate ...   165   1e-38
ref|XP_014521655.1| PREDICTED: probable transmembrane ascorbate ...   164   2e-38
ref|XP_002283161.1| PREDICTED: probable transmembrane ascorbate ...   164   2e-38
emb|CAN62730.1| hypothetical protein VITISV_001339 [Vitis vinife...   164   2e-38
gb|KOM56071.1| hypothetical protein LR48_Vigan10g196300 [Vigna a...   164   3e-38
gb|KRG93827.1| hypothetical protein GLYMA_19G044200 [Glycine max]     163   4e-38
ref|XP_007033346.1| Cytochrome B561-1 isoform 3 [Theobroma cacao...   163   4e-38
ref|XP_007033345.1| Cytochrome B561-1 isoform 2 [Theobroma cacao...   163   4e-38
ref|XP_007033344.1| Cytochrome B561-1 isoform 1 [Theobroma cacao...   163   4e-38
ref|XP_003555094.1| PREDICTED: probable transmembrane ascorbate ...   163   4e-38

>gb|KDO58932.1| hypothetical protein CISIN_1g0268681mg [Citrus sinensis]
           gi|641840010|gb|KDO58933.1| hypothetical protein
           CISIN_1g0268681mg, partial [Citrus sinensis]
           gi|641840011|gb|KDO58934.1| hypothetical protein
           CISIN_1g0268681mg, partial [Citrus sinensis]
          Length = 166

 Score =  181 bits (458), Expect = 2e-43
 Identities = 89/100 (89%), Positives = 94/100 (94%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWAAGFTTFWYPGGSKN RASLLPWHVF GVYIY LAVATAATGILEKVTFLQTN+VISR
Sbjct: 66  QWAAGFTTFWYPGGSKNGRASLLPWHVFLGVYIYALAVATAATGILEKVTFLQTNHVISR 125

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFKAS 79
           YSTEALLVNSLGILI VLGGFVILAL+TP +GKS+V +AS
Sbjct: 126 YSTEALLVNSLGILIVVLGGFVILALITPENGKSEVLRAS 165


>ref|XP_006482300.1| PREDICTED: probable transmembrane ascorbate ferrireductase 2-like
           isoform X1 [Citrus sinensis]
           gi|568857495|ref|XP_006482301.1| PREDICTED: probable
           transmembrane ascorbate ferrireductase 2-like isoform X2
           [Citrus sinensis]
          Length = 231

 Score =  181 bits (458), Expect = 2e-43
 Identities = 89/100 (89%), Positives = 94/100 (94%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWAAGFTTFWYPGGSKN RASLLPWHVF GVYIY LAVATAATGILEKVTFLQTN+VISR
Sbjct: 131 QWAAGFTTFWYPGGSKNGRASLLPWHVFLGVYIYALAVATAATGILEKVTFLQTNHVISR 190

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFKAS 79
           YSTEALLVNSLGILI VLGGFVILAL+TP +GKS+V +AS
Sbjct: 191 YSTEALLVNSLGILIVVLGGFVILALITPENGKSEVLRAS 230


>ref|XP_006430824.1| hypothetical protein CICLE_v10012646mg [Citrus clementina]
           gi|557532881|gb|ESR44064.1| hypothetical protein
           CICLE_v10012646mg [Citrus clementina]
          Length = 231

 Score =  181 bits (458), Expect = 2e-43
 Identities = 89/100 (89%), Positives = 94/100 (94%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWAAGFTTFWYPGGSKN RASLLPWHVF GVYIY LAVATAATGILEKVTFLQTN+VISR
Sbjct: 131 QWAAGFTTFWYPGGSKNGRASLLPWHVFLGVYIYALAVATAATGILEKVTFLQTNHVISR 190

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFKAS 79
           YSTEALLVNSLGILI VLGGFVILAL+TP +GKS+V +AS
Sbjct: 191 YSTEALLVNSLGILIVVLGGFVILALITPENGKSEVLRAS 230


>ref|XP_006430823.1| hypothetical protein CICLE_v10012646mg [Citrus clementina]
           gi|557532880|gb|ESR44063.1| hypothetical protein
           CICLE_v10012646mg [Citrus clementina]
          Length = 166

 Score =  181 bits (458), Expect = 2e-43
 Identities = 89/100 (89%), Positives = 94/100 (94%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWAAGFTTFWYPGGSKN RASLLPWHVF GVYIY LAVATAATGILEKVTFLQTN+VISR
Sbjct: 66  QWAAGFTTFWYPGGSKNGRASLLPWHVFLGVYIYALAVATAATGILEKVTFLQTNHVISR 125

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFKAS 79
           YSTEALLVNSLGILI VLGGFVILAL+TP +GKS+V +AS
Sbjct: 126 YSTEALLVNSLGILIVVLGGFVILALITPENGKSEVLRAS 165


>ref|XP_007151758.1| hypothetical protein PHAVU_004G072300g [Phaseolus vulgaris]
           gi|561025067|gb|ESW23752.1| hypothetical protein
           PHAVU_004G072300g [Phaseolus vulgaris]
          Length = 232

 Score =  169 bits (429), Expect = 6e-40
 Identities = 78/100 (78%), Positives = 88/100 (88%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWAAGF TFWYPGGS+NRRA+LLPWHVFFG+YIY LA+ATAATGILEK TFLQ NN+ISR
Sbjct: 132 QWAAGFATFWYPGGSRNRRATLLPWHVFFGIYIYALAIATAATGILEKATFLQVNNIISR 191

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFKAS 79
           YS EALL+NSLGILI +LGGFVIL LVTP  GK+ V + +
Sbjct: 192 YSNEALLINSLGILIVILGGFVILGLVTPVYGKADVIRGN 231


>ref|XP_012471058.1| PREDICTED: probable transmembrane ascorbate ferrireductase 2
           [Gossypium raimondii] gi|763738796|gb|KJB06295.1|
           hypothetical protein B456_001G081800 [Gossypium
           raimondii]
          Length = 240

 Score =  166 bits (421), Expect = 5e-39
 Identities = 80/98 (81%), Positives = 87/98 (88%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWAAGF TFWYPGGS+N RA+LLPWHVFFG+Y Y LAVATA TGILEK+TFLQTN VISR
Sbjct: 139 QWAAGFATFWYPGGSRNSRAALLPWHVFFGMYTYALAVATATTGILEKLTFLQTNRVISR 198

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFK 85
           YSTEALLVNSLGILI VLGGFVILA +TP +GKS V +
Sbjct: 199 YSTEALLVNSLGILIVVLGGFVILATLTPVNGKSDVHR 236


>gb|KJB06294.1| hypothetical protein B456_001G081800 [Gossypium raimondii]
          Length = 182

 Score =  166 bits (421), Expect = 5e-39
 Identities = 80/98 (81%), Positives = 87/98 (88%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWAAGF TFWYPGGS+N RA+LLPWHVFFG+Y Y LAVATA TGILEK+TFLQTN VISR
Sbjct: 81  QWAAGFATFWYPGGSRNSRAALLPWHVFFGMYTYALAVATATTGILEKLTFLQTNRVISR 140

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFK 85
           YSTEALLVNSLGILI VLGGFVILA +TP +GKS V +
Sbjct: 141 YSTEALLVNSLGILIVVLGGFVILATLTPVNGKSDVHR 178


>gb|KJB06293.1| hypothetical protein B456_001G081800 [Gossypium raimondii]
          Length = 205

 Score =  166 bits (421), Expect = 5e-39
 Identities = 80/98 (81%), Positives = 87/98 (88%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWAAGF TFWYPGGS+N RA+LLPWHVFFG+Y Y LAVATA TGILEK+TFLQTN VISR
Sbjct: 104 QWAAGFATFWYPGGSRNSRAALLPWHVFFGMYTYALAVATATTGILEKLTFLQTNRVISR 163

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFK 85
           YSTEALLVNSLGILI VLGGFVILA +TP +GKS V +
Sbjct: 164 YSTEALLVNSLGILIVVLGGFVILATLTPVNGKSDVHR 201


>emb|CDP02400.1| unnamed protein product [Coffea canephora]
          Length = 232

 Score =  166 bits (420), Expect = 6e-39
 Identities = 78/100 (78%), Positives = 88/100 (88%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWAAGF TFWYPGGS+N RASLLPWHVFFGVYIY LA+AT  TGILEK TFLQTN ++SR
Sbjct: 131 QWAAGFVTFWYPGGSRNSRASLLPWHVFFGVYIYALAIATCTTGILEKATFLQTNQIMSR 190

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFKAS 79
           YS EALLVNSLGILI  LGGFVILA+++PA+GKS V K++
Sbjct: 191 YSKEALLVNSLGILIVALGGFVILAVISPANGKSDVTKST 230


>ref|XP_010692475.1| PREDICTED: probable transmembrane ascorbate ferrireductase 2
           isoform X2 [Beta vulgaris subsp. vulgaris]
          Length = 204

 Score =  165 bits (418), Expect = 1e-38
 Identities = 77/96 (80%), Positives = 85/96 (88%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWA GFTTFWYPGGS+N R+SLLPWHVFFGVYIY LA+ T  TGILEK TFLQTN+VISR
Sbjct: 104 QWAGGFTTFWYPGGSRNNRSSLLPWHVFFGVYIYALAIVTTVTGILEKATFLQTNHVISR 163

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQV 91
           YSTEALLVNS+GILI VLGGFVILALV P +GK ++
Sbjct: 164 YSTEALLVNSMGILIVVLGGFVILALVAPLNGKGEI 199


>ref|XP_010692471.1| PREDICTED: probable transmembrane ascorbate ferrireductase 2
           isoform X1 [Beta vulgaris subsp. vulgaris]
           gi|870867625|gb|KMT18494.1| hypothetical protein
           BVRB_2g026350 [Beta vulgaris subsp. vulgaris]
          Length = 231

 Score =  165 bits (418), Expect = 1e-38
 Identities = 77/96 (80%), Positives = 85/96 (88%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWA GFTTFWYPGGS+N R+SLLPWHVFFGVYIY LA+ T  TGILEK TFLQTN+VISR
Sbjct: 131 QWAGGFTTFWYPGGSRNNRSSLLPWHVFFGVYIYALAIVTTVTGILEKATFLQTNHVISR 190

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQV 91
           YSTEALLVNS+GILI VLGGFVILALV P +GK ++
Sbjct: 191 YSTEALLVNSMGILIVVLGGFVILALVAPLNGKGEI 226


>ref|XP_014521655.1| PREDICTED: probable transmembrane ascorbate ferrireductase 2 [Vigna
           radiata var. radiata] gi|951067469|ref|XP_014524238.1|
           PREDICTED: probable transmembrane ascorbate
           ferrireductase 2 [Vigna radiata var. radiata]
          Length = 232

 Score =  164 bits (416), Expect = 2e-38
 Identities = 75/100 (75%), Positives = 86/100 (86%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWAAGF TFWYPGGS+NRRA+LLPWHVFFG+YIY LA+AT ATGILEK TFLQ N +ISR
Sbjct: 132 QWAAGFATFWYPGGSRNRRATLLPWHVFFGMYIYALAIATTATGILEKATFLQVNKIISR 191

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFKAS 79
           YS EALL+NSLGILI +LGGFVIL L+TP  GK+ V + +
Sbjct: 192 YSNEALLINSLGILIVILGGFVILGLITPVYGKADVIRGN 231


>ref|XP_002283161.1| PREDICTED: probable transmembrane ascorbate ferrireductase 2 [Vitis
           vinifera]
          Length = 265

 Score =  164 bits (416), Expect = 2e-38
 Identities = 80/100 (80%), Positives = 86/100 (86%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWA GF TFWYPGGS+N RASLLPWHVFF VYIY LAVATA TGILEK TFLQ+N VISR
Sbjct: 164 QWATGFATFWYPGGSRNSRASLLPWHVFFAVYIYALAVATATTGILEKATFLQSNKVISR 223

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFKAS 79
           YS EALLVNSLGILI VLGGFVILA+VTPA+GK  + + S
Sbjct: 224 YSMEALLVNSLGILIVVLGGFVILAVVTPANGKGDIPRGS 263


>emb|CAN62730.1| hypothetical protein VITISV_001339 [Vitis vinifera]
           gi|298204382|emb|CBI16862.3| unnamed protein product
           [Vitis vinifera]
          Length = 232

 Score =  164 bits (416), Expect = 2e-38
 Identities = 80/100 (80%), Positives = 86/100 (86%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWA GF TFWYPGGS+N RASLLPWHVFF VYIY LAVATA TGILEK TFLQ+N VISR
Sbjct: 131 QWATGFATFWYPGGSRNSRASLLPWHVFFAVYIYALAVATATTGILEKATFLQSNKVISR 190

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFKAS 79
           YS EALLVNSLGILI VLGGFVILA+VTPA+GK  + + S
Sbjct: 191 YSMEALLVNSLGILIVVLGGFVILAVVTPANGKGDIPRGS 230


>gb|KOM56071.1| hypothetical protein LR48_Vigan10g196300 [Vigna angularis]
          Length = 284

 Score =  164 bits (414), Expect = 3e-38
 Identities = 74/100 (74%), Positives = 86/100 (86%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWAAGF TFWYPGGS+NRRA+L+PWHVFFG+YIY LA+AT ATGILEK TFLQ N +ISR
Sbjct: 184 QWAAGFATFWYPGGSRNRRATLMPWHVFFGMYIYALAIATTATGILEKATFLQVNKIISR 243

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFKAS 79
           YS EALL+NSLGILI +LGGFVIL L+TP  GK+ V + +
Sbjct: 244 YSNEALLINSLGILIVILGGFVILGLITPVYGKADVIRGN 283


>gb|KRG93827.1| hypothetical protein GLYMA_19G044200 [Glycine max]
          Length = 178

 Score =  163 bits (413), Expect = 4e-38
 Identities = 75/100 (75%), Positives = 85/100 (85%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QW AGF TFWYPGGS+N RA+LLPWHVFFG+YIY LA+AT ATGILEK TFLQ NN+ISR
Sbjct: 78  QWGAGFATFWYPGGSRNSRAALLPWHVFFGIYIYCLAIATTATGILEKATFLQVNNIISR 137

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFKAS 79
           YS EALLVNSLGILI +LGGFVIL +VTP  GK+ V + +
Sbjct: 138 YSNEALLVNSLGILIVILGGFVILGIVTPVYGKADVIRGN 177


>ref|XP_007033346.1| Cytochrome B561-1 isoform 3 [Theobroma cacao]
           gi|508712375|gb|EOY04272.1| Cytochrome B561-1 isoform 3
           [Theobroma cacao]
          Length = 177

 Score =  163 bits (413), Expect = 4e-38
 Identities = 79/98 (80%), Positives = 86/98 (87%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWAAGF TFWYPGGS+N RA+LLPWHVFFG+Y Y LAVATA TGILEK+TFLQTN VISR
Sbjct: 76  QWAAGFATFWYPGGSRNGRAALLPWHVFFGMYTYALAVATATTGILEKLTFLQTNLVISR 135

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFK 85
           YSTEALLVNSLGILI VLGGFVILA +TP +GKS   +
Sbjct: 136 YSTEALLVNSLGILIVVLGGFVILATLTPVNGKSDAHR 173


>ref|XP_007033345.1| Cytochrome B561-1 isoform 2 [Theobroma cacao]
           gi|508712374|gb|EOY04271.1| Cytochrome B561-1 isoform 2
           [Theobroma cacao]
          Length = 167

 Score =  163 bits (413), Expect = 4e-38
 Identities = 79/98 (80%), Positives = 86/98 (87%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWAAGF TFWYPGGS+N RA+LLPWHVFFG+Y Y LAVATA TGILEK+TFLQTN VISR
Sbjct: 66  QWAAGFATFWYPGGSRNGRAALLPWHVFFGMYTYALAVATATTGILEKLTFLQTNLVISR 125

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFK 85
           YSTEALLVNSLGILI VLGGFVILA +TP +GKS   +
Sbjct: 126 YSTEALLVNSLGILIVVLGGFVILATLTPVNGKSDAHR 163


>ref|XP_007033344.1| Cytochrome B561-1 isoform 1 [Theobroma cacao]
           gi|508712373|gb|EOY04270.1| Cytochrome B561-1 isoform 1
           [Theobroma cacao]
          Length = 232

 Score =  163 bits (413), Expect = 4e-38
 Identities = 79/98 (80%), Positives = 86/98 (87%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QWAAGF TFWYPGGS+N RA+LLPWHVFFG+Y Y LAVATA TGILEK+TFLQTN VISR
Sbjct: 131 QWAAGFATFWYPGGSRNGRAALLPWHVFFGMYTYALAVATATTGILEKLTFLQTNLVISR 190

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFK 85
           YSTEALLVNSLGILI VLGGFVILA +TP +GKS   +
Sbjct: 191 YSTEALLVNSLGILIVVLGGFVILATLTPVNGKSDAHR 228


>ref|XP_003555094.1| PREDICTED: probable transmembrane ascorbate ferrireductase 2
           [Glycine max] gi|734393809|gb|KHN28344.1| Putative
           transmembrane ascorbate ferrireductase 2 [Glycine soja]
           gi|947044196|gb|KRG93825.1| hypothetical protein
           GLYMA_19G044200 [Glycine max]
          Length = 232

 Score =  163 bits (413), Expect = 4e-38
 Identities = 75/100 (75%), Positives = 85/100 (85%)
 Frame = -3

Query: 378 QWAAGFTTFWYPGGSKNRRASLLPWHVFFGVYIYVLAVATAATGILEKVTFLQTNNVISR 199
           QW AGF TFWYPGGS+N RA+LLPWHVFFG+YIY LA+AT ATGILEK TFLQ NN+ISR
Sbjct: 132 QWGAGFATFWYPGGSRNSRAALLPWHVFFGIYIYCLAIATTATGILEKATFLQVNNIISR 191

Query: 198 YSTEALLVNSLGILIFVLGGFVILALVTPASGKSQVFKAS 79
           YS EALLVNSLGILI +LGGFVIL +VTP  GK+ V + +
Sbjct: 192 YSNEALLVNSLGILIVILGGFVILGIVTPVYGKADVIRGN 231