BLASTX nr result
ID: Wisteria21_contig00039458
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Wisteria21_contig00039458
(423 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_014499931.1| PREDICTED: uncharacterized protein LOC106760... 188 1e-45
gb|KOM43529.1| hypothetical protein LR48_Vigan05g113300 [Vigna a... 188 1e-45
ref|XP_006591846.1| PREDICTED: uncharacterized protein LOC100527... 185 1e-44
ref|XP_007149427.1| hypothetical protein PHAVU_005G069500g [Phas... 181 2e-43
ref|XP_004517128.1| PREDICTED: uncharacterized protein LOC101512... 179 9e-43
ref|XP_003541833.1| PREDICTED: uncharacterized protein LOC100803... 173 5e-41
ref|XP_013464639.1| hypothetical protein MTR_2g078620 [Medicago ... 169 6e-40
emb|CBI20241.3| unnamed protein product [Vitis vinifera] 148 2e-33
ref|XP_002285236.1| PREDICTED: uncharacterized protein LOC100257... 148 2e-33
emb|CAN63558.1| hypothetical protein VITISV_034122 [Vitis vinifera] 148 2e-33
ref|XP_006451767.1| hypothetical protein CICLE_v10010201mg [Citr... 142 1e-31
ref|XP_010279224.1| PREDICTED: uncharacterized protein LOC104613... 137 2e-30
ref|XP_007213579.1| hypothetical protein PRUPE_ppa014990mg [Prun... 137 4e-30
ref|XP_008226466.1| PREDICTED: uncharacterized protein LOC103326... 134 3e-29
ref|XP_004294142.1| PREDICTED: uncharacterized protein LOC101306... 131 2e-28
ref|XP_007021600.1| Serine/arginine repetitive matrix protein 2,... 130 5e-28
ref|XP_010098450.1| hypothetical protein L484_012165 [Morus nota... 129 7e-28
ref|XP_010062846.1| PREDICTED: uncharacterized protein LOC104450... 129 1e-27
ref|XP_009339321.1| PREDICTED: uncharacterized protein LOC103931... 126 7e-27
ref|XP_012457927.1| PREDICTED: uncharacterized protein LOC105778... 125 1e-26
>ref|XP_014499931.1| PREDICTED: uncharacterized protein LOC106760983 [Vigna radiata var.
radiata]
Length = 336
Score = 188 bits (478), Expect = 1e-45
Identities = 92/102 (90%), Positives = 95/102 (93%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
RRSLSDLE+EEVQGFKDLGFSFEKE LSP+LANILPGLQEK RDETEEDKAAR PYLSEA
Sbjct: 236 RRSLSDLEYEEVQGFKDLGFSFEKETLSPSLANILPGLQEKKRDETEEDKAARRPYLSEA 295
Query: 241 WLVQSCAPPVPNWAPNNISAADMKQQIKFWARAVASNVHQEC 116
WLVQSCAPP+PNWA N SA DMKQQIKFWARAVASNVHQEC
Sbjct: 296 WLVQSCAPPIPNWASNK-SAGDMKQQIKFWARAVASNVHQEC 336
>gb|KOM43529.1| hypothetical protein LR48_Vigan05g113300 [Vigna angularis]
Length = 332
Score = 188 bits (478), Expect = 1e-45
Identities = 92/102 (90%), Positives = 95/102 (93%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
RRSLSDLE+EEVQGFKDLGFSFEKE LSP+LANILPGLQEK RDETEEDKAAR PYLSEA
Sbjct: 232 RRSLSDLEYEEVQGFKDLGFSFEKETLSPSLANILPGLQEKKRDETEEDKAARRPYLSEA 291
Query: 241 WLVQSCAPPVPNWAPNNISAADMKQQIKFWARAVASNVHQEC 116
WLVQSCAPP+PNWA N SA DMKQQIKFWARAVASNVHQEC
Sbjct: 292 WLVQSCAPPIPNWASNK-SAGDMKQQIKFWARAVASNVHQEC 332
>ref|XP_006591846.1| PREDICTED: uncharacterized protein LOC100527250 isoform X1 [Glycine
max] gi|734393168|gb|KHN28084.1| hypothetical protein
glysoja_007816 [Glycine soja]
gi|947078451|gb|KRH27291.1| hypothetical protein
GLYMA_12G227100 [Glycine max]
Length = 331
Score = 185 bits (469), Expect = 1e-44
Identities = 90/102 (88%), Positives = 96/102 (94%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
RRSLSDLEFEEVQGFKDLGFSFEKEALSP+LA+ILPGLQEK RDETEEDKAAR PYLSEA
Sbjct: 231 RRSLSDLEFEEVQGFKDLGFSFEKEALSPSLASILPGLQEKKRDETEEDKAARRPYLSEA 290
Query: 241 WLVQSCAPPVPNWAPNNISAADMKQQIKFWARAVASNVHQEC 116
WLVQSCAPP+PNWA + S+ DMK+QIKFWARAVASNVHQEC
Sbjct: 291 WLVQSCAPPIPNWASHK-SSGDMKEQIKFWARAVASNVHQEC 331
>ref|XP_007149427.1| hypothetical protein PHAVU_005G069500g [Phaseolus vulgaris]
gi|561022691|gb|ESW21421.1| hypothetical protein
PHAVU_005G069500g [Phaseolus vulgaris]
Length = 317
Score = 181 bits (459), Expect = 2e-43
Identities = 91/102 (89%), Positives = 94/102 (92%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
RRSLSDLE+EEVQGFKDLGFSFEKE LSP+LANILPGLQEK RDETEEDKAAR PYLSEA
Sbjct: 218 RRSLSDLEYEEVQGFKDLGFSFEKETLSPSLANILPGLQEKKRDETEEDKAARRPYLSEA 277
Query: 241 WLVQSCAPPVPNWAPNNISAADMKQQIKFWARAVASNVHQEC 116
WLVQSCA P+PNWA N SA DMKQQIKFWARAVASNVHQEC
Sbjct: 278 WLVQSCA-PIPNWASNK-SAGDMKQQIKFWARAVASNVHQEC 317
>ref|XP_004517128.1| PREDICTED: uncharacterized protein LOC101512525 [Cicer arietinum]
Length = 317
Score = 179 bits (453), Expect = 9e-43
Identities = 88/103 (85%), Positives = 94/103 (91%), Gaps = 1/103 (0%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
RRSLSDLE EEVQGFKDLGFSFEKE LSP+LANI+PGLQEKNRDE+EEDKAARGPYLSEA
Sbjct: 215 RRSLSDLELEEVQGFKDLGFSFEKEGLSPSLANIIPGLQEKNRDESEEDKAARGPYLSEA 274
Query: 241 WLVQS-CAPPVPNWAPNNISAADMKQQIKFWARAVASNVHQEC 116
WLVQS CAPPVPN N+S AD+K+ IKFWARAVASNVHQEC
Sbjct: 275 WLVQSCCAPPVPNCGNTNMSKADIKKNIKFWARAVASNVHQEC 317
>ref|XP_003541833.1| PREDICTED: uncharacterized protein LOC100803315 [Glycine max]
gi|734413412|gb|KHN36857.1| hypothetical protein
glysoja_001588 [Glycine soja]
gi|947073136|gb|KRH22027.1| hypothetical protein
GLYMA_13G273200 [Glycine max]
Length = 334
Score = 173 bits (438), Expect = 5e-41
Identities = 85/102 (83%), Positives = 91/102 (89%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
RRSLSDLEFEEVQGFKDLGFSFEKE LSP+LA+ILPGLQEK RDETEEDKAAR PYLSEA
Sbjct: 234 RRSLSDLEFEEVQGFKDLGFSFEKETLSPSLASILPGLQEKKRDETEEDKAARRPYLSEA 293
Query: 241 WLVQSCAPPVPNWAPNNISAADMKQQIKFWARAVASNVHQEC 116
WLVQSCAP +PNW + S+ DMK QIKFWARAVASNVH +C
Sbjct: 294 WLVQSCAPAIPNWTSHK-SSGDMKVQIKFWARAVASNVHLKC 334
>ref|XP_013464639.1| hypothetical protein MTR_2g078620 [Medicago truncatula]
gi|657399188|gb|KEH38674.1| hypothetical protein
MTR_2g078620 [Medicago truncatula]
Length = 322
Score = 169 bits (429), Expect = 6e-40
Identities = 85/97 (87%), Positives = 91/97 (93%), Gaps = 1/97 (1%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
RRSLSDLE+EEVQGFKDLGFSFEKEALSP+LANI+PGLQEKNRDE+EEDKAARGPYLSEA
Sbjct: 224 RRSLSDLEYEEVQGFKDLGFSFEKEALSPSLANIIPGLQEKNRDESEEDKAARGPYLSEA 283
Query: 241 WLVQSCA-PPVPNWAPNNISAADMKQQIKFWARAVAS 134
WLVQSCA PPVPNW N +SA DMK+ IKFWARAVAS
Sbjct: 284 WLVQSCATPPVPNWG-NKMSADDMKKHIKFWARAVAS 319
>emb|CBI20241.3| unnamed protein product [Vitis vinifera]
Length = 184
Score = 148 bits (373), Expect = 2e-33
Identities = 72/102 (70%), Positives = 83/102 (81%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
R+SLSDLE+EEVQGFKDLGF+FEKE LSP++ NILPGLQ K+R E+ + R PYLSEA
Sbjct: 84 RKSLSDLEYEEVQGFKDLGFTFEKEDLSPSVVNILPGLQVKDRGGPVEEDSVRRPYLSEA 143
Query: 241 WLVQSCAPPVPNWAPNNISAADMKQQIKFWARAVASNVHQEC 116
W+ Q APP+PNW + SA DMK QIKFWARAVASNVHQEC
Sbjct: 144 WIEQCSAPPIPNWVGKS-SAQDMKAQIKFWARAVASNVHQEC 184
>ref|XP_002285236.1| PREDICTED: uncharacterized protein LOC100257151 [Vitis vinifera]
Length = 399
Score = 148 bits (373), Expect = 2e-33
Identities = 72/102 (70%), Positives = 83/102 (81%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
R+SLSDLE+EEVQGFKDLGF+FEKE LSP++ NILPGLQ K+R E+ + R PYLSEA
Sbjct: 299 RKSLSDLEYEEVQGFKDLGFTFEKEDLSPSVVNILPGLQVKDRGGPVEEDSVRRPYLSEA 358
Query: 241 WLVQSCAPPVPNWAPNNISAADMKQQIKFWARAVASNVHQEC 116
W+ Q APP+PNW + SA DMK QIKFWARAVASNVHQEC
Sbjct: 359 WIEQCSAPPIPNWVGKS-SAQDMKAQIKFWARAVASNVHQEC 399
>emb|CAN63558.1| hypothetical protein VITISV_034122 [Vitis vinifera]
Length = 399
Score = 148 bits (373), Expect = 2e-33
Identities = 72/102 (70%), Positives = 83/102 (81%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
R+SLSDLE+EEVQGFKDLGF+FEKE LSP++ NILPGLQ K+R E+ + R PYLSEA
Sbjct: 299 RKSLSDLEYEEVQGFKDLGFTFEKEDLSPSVVNILPGLQVKDRGGPVEEDSVRRPYLSEA 358
Query: 241 WLVQSCAPPVPNWAPNNISAADMKQQIKFWARAVASNVHQEC 116
W+ Q APP+PNW + SA DMK QIKFWARAVASNVHQEC
Sbjct: 359 WIEQCSAPPIPNWVGKS-SAQDMKAQIKFWARAVASNVHQEC 399
>ref|XP_006451767.1| hypothetical protein CICLE_v10010201mg [Citrus clementina]
gi|568820648|ref|XP_006464821.1| PREDICTED:
uncharacterized protein LOC102621220 [Citrus sinensis]
gi|557554993|gb|ESR65007.1| hypothetical protein
CICLE_v10010201mg [Citrus clementina]
gi|641839601|gb|KDO58529.1| hypothetical protein
CISIN_1g018815mg [Citrus sinensis]
Length = 350
Score = 142 bits (358), Expect = 1e-31
Identities = 73/106 (68%), Positives = 86/106 (81%), Gaps = 4/106 (3%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDE-TEEDKAARGPYLSE 245
R+SL+DLE EEVQGFKDLGF+FEK+ LSPN+ NILPGLQEK +++ ++ D R PYLSE
Sbjct: 246 RKSLNDLETEEVQGFKDLGFTFEKKDLSPNVVNILPGLQEKKQEKLSQMDYKMRRPYLSE 305
Query: 244 AWLVQSCAP---PVPNWAPNNISAADMKQQIKFWARAVASNVHQEC 116
AW+VQSCAP PVPN P N S DMK+Q+KFWARAVASNV QEC
Sbjct: 306 AWVVQSCAPPAAPVPNCVPKN-STEDMKEQLKFWARAVASNVRQEC 350
>ref|XP_010279224.1| PREDICTED: uncharacterized protein LOC104613196 [Nelumbo nucifera]
Length = 282
Score = 137 bits (346), Expect = 2e-30
Identities = 66/102 (64%), Positives = 78/102 (76%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
R+SLSDLEFEE+QGFKDLGF+F KE LSP + NI+P L+EK + +ED R PYLSEA
Sbjct: 182 RKSLSDLEFEELQGFKDLGFTFNKEDLSPTVVNIIPALKEKTQQNWDEDNKVRRPYLSEA 241
Query: 241 WLVQSCAPPVPNWAPNNISAADMKQQIKFWARAVASNVHQEC 116
WLVQ A P+PNW ++ SA DMK +KFWAR VASNV QEC
Sbjct: 242 WLVQRSASPMPNWV-HSESAGDMKAHLKFWARVVASNVRQEC 282
>ref|XP_007213579.1| hypothetical protein PRUPE_ppa014990mg [Prunus persica]
gi|462409444|gb|EMJ14778.1| hypothetical protein
PRUPE_ppa014990mg [Prunus persica]
Length = 333
Score = 137 bits (344), Expect = 4e-30
Identities = 69/98 (70%), Positives = 78/98 (79%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
R+SLSDLE EE+QGFKDLGF+F+K+ LSP++ NILPGLQEK R E + R PYLSEA
Sbjct: 236 RKSLSDLEIEELQGFKDLGFTFDKKELSPSVVNILPGLQEKKRTEDLNPEKVRRPYLSEA 295
Query: 241 WLVQSCAPPVPNWAPNNISAADMKQQIKFWARAVASNV 128
WLVQSCAPP PN + SA DMK QIKFWARAVASNV
Sbjct: 296 WLVQSCAPPPPNLGASR-SAEDMKAQIKFWARAVASNV 332
>ref|XP_008226466.1| PREDICTED: uncharacterized protein LOC103326040 [Prunus mume]
Length = 333
Score = 134 bits (337), Expect = 3e-29
Identities = 68/98 (69%), Positives = 77/98 (78%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
R+SLSDLE EE+QGFKDLGF+F+K+ LSP++ NILPGLQEK E + R PYLSEA
Sbjct: 236 RKSLSDLEIEELQGFKDLGFTFDKKELSPSVVNILPGLQEKKGTEDLNPEKVRRPYLSEA 295
Query: 241 WLVQSCAPPVPNWAPNNISAADMKQQIKFWARAVASNV 128
WLVQSCAPP PN + SA DMK QIKFWARAVASNV
Sbjct: 296 WLVQSCAPPPPNLGASR-SAEDMKAQIKFWARAVASNV 332
>ref|XP_004294142.1| PREDICTED: uncharacterized protein LOC101306310 [Fragaria vesca
subsp. vesca]
Length = 307
Score = 131 bits (329), Expect = 2e-28
Identities = 66/98 (67%), Positives = 77/98 (78%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
R+SLSDLE EEVQGFKDLGF+F+K+ ++P++ +ILPGLQEK R+E R PYLSEA
Sbjct: 210 RKSLSDLEIEEVQGFKDLGFTFDKKDIAPSVVSILPGLQEKKRNEELNLDMVRRPYLSEA 269
Query: 241 WLVQSCAPPVPNWAPNNISAADMKQQIKFWARAVASNV 128
WLVQSCAPP PN SA DMK QIKFWAR+VASNV
Sbjct: 270 WLVQSCAPPPPNLGAGR-SAEDMKAQIKFWARSVASNV 306
>ref|XP_007021600.1| Serine/arginine repetitive matrix protein 2, putative [Theobroma
cacao] gi|508721228|gb|EOY13125.1| Serine/arginine
repetitive matrix protein 2, putative [Theobroma cacao]
Length = 337
Score = 130 bits (326), Expect = 5e-28
Identities = 66/100 (66%), Positives = 79/100 (79%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
+RSLSDL FEE+QGFKDLGF+F+KE LSP++ NILPGLQE ++ ++DK R PYLSEA
Sbjct: 240 QRSLSDLAFEELQGFKDLGFTFDKEDLSPSVVNILPGLQENKIEDLKQDKVRR-PYLSEA 298
Query: 241 WLVQSCAPPVPNWAPNNISAADMKQQIKFWARAVASNVHQ 122
WL QS PP+PN + SA DMK QIKFWARAVA+NV Q
Sbjct: 299 WLAQSRGPPIPNCVSKD-SADDMKAQIKFWARAVATNVRQ 337
>ref|XP_010098450.1| hypothetical protein L484_012165 [Morus notabilis]
gi|587886220|gb|EXB75041.1| hypothetical protein
L484_012165 [Morus notabilis]
Length = 353
Score = 129 bits (325), Expect = 7e-28
Identities = 66/102 (64%), Positives = 78/102 (76%), Gaps = 1/102 (0%)
Frame = -3
Query: 418 RSLSDLEFEEVQGFKDLGFSFE-KEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
RSLS+LE EEVQGFKDLGF F+ K+ LSPN+ NILPGLQE+ ++ R PYLSEA
Sbjct: 254 RSLSELEIEEVQGFKDLGFKFDNKDLLSPNVVNILPGLQERKEEDMGLQNKVRRPYLSEA 313
Query: 241 WLVQSCAPPVPNWAPNNISAADMKQQIKFWARAVASNVHQEC 116
W+ QS APP PNWA + S+ +MK QIKFWAR+VASNV QEC
Sbjct: 314 WMAQS-APPTPNWAASR-SSQEMKAQIKFWARSVASNVRQEC 353
>ref|XP_010062846.1| PREDICTED: uncharacterized protein LOC104450110 [Eucalyptus
grandis] gi|629104513|gb|KCW69982.1| hypothetical
protein EUGRSUZ_F03292 [Eucalyptus grandis]
Length = 349
Score = 129 bits (323), Expect = 1e-27
Identities = 65/102 (63%), Positives = 74/102 (72%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
R+SLSDLE EEV+GFK+LGFSFE++ L+P L +ILPGLQEK + E E+ PYLSE
Sbjct: 249 RKSLSDLEDEEVRGFKELGFSFEEKHLNPKLVDILPGLQEKTQ-ENEDGNGNEAPYLSEG 307
Query: 241 WLVQSCAPPVPNWAPNNISAADMKQQIKFWARAVASNVHQEC 116
WL QS AP VP W S DMK QIKFWAR VASNV QEC
Sbjct: 308 WLAQSYAPAVPKWNAKGGSPEDMKAQIKFWARVVASNVRQEC 349
>ref|XP_009339321.1| PREDICTED: uncharacterized protein LOC103931537 [Pyrus x
bretschneideri]
Length = 324
Score = 126 bits (316), Expect = 7e-27
Identities = 66/99 (66%), Positives = 73/99 (73%), Gaps = 1/99 (1%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQEKNRDETEEDKAARGPYLSEA 242
R+SLSDLE EE+QGFKDLGF+F+KE LSP++ NILPGLQEK E R PYLSE
Sbjct: 226 RKSLSDLEIEELQGFKDLGFTFDKEELSPSVVNILPGLQEKKTSEDLNPAHVRRPYLSEV 285
Query: 241 WLVQSC-APPVPNWAPNNISAADMKQQIKFWARAVASNV 128
WL QSC APP PN SA DMK QIKFWAR+VASNV
Sbjct: 286 WLAQSCAAPPPPNLGATK-SAEDMKAQIKFWARSVASNV 323
>ref|XP_012457927.1| PREDICTED: uncharacterized protein LOC105778718 [Gossypium
raimondii] gi|763745844|gb|KJB13283.1| hypothetical
protein B456_002G066500 [Gossypium raimondii]
Length = 308
Score = 125 bits (314), Expect = 1e-26
Identities = 67/104 (64%), Positives = 80/104 (76%), Gaps = 2/104 (1%)
Frame = -3
Query: 421 RRSLSDLEFEEVQGFKDLGFSFEKEALSPNLANILPGLQ-EKNRDETEEDKAARGPYLSE 245
++S SDL F+E+QGFKDLGF+F+KE LSP++ NILPGLQ +K DE + DK + PYLSE
Sbjct: 207 QKSYSDLAFQELQGFKDLGFTFDKEDLSPDIVNILPGLQGDKIEDELQPDKVKK-PYLSE 265
Query: 244 AWLVQSCA-PPVPNWAPNNISAADMKQQIKFWARAVASNVHQEC 116
AWLVQ A PP+P N SA DMK QIKFWARAVA+NV QEC
Sbjct: 266 AWLVQGPAPPPIPTCVSKN-SAKDMKAQIKFWARAVATNVRQEC 308