BLASTX nr result
ID: Stemona21_contig00040683
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Stemona21_contig00040683
(316 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AFW76830.1| hypothetical protein ZEAMMB73_573219 [Zea mays] 160 1e-37
gb|AFW76829.1| ROS1 protein [Zea mays] 160 1e-37
gb|AFW76828.1| hypothetical protein ZEAMMB73_573219 [Zea mays] 160 1e-37
gb|ACG42155.1| ROS1 protein [Zea mays] gi|219887377|gb|ACL54063.... 160 1e-37
ref|XP_006655955.1| PREDICTED: DEMETER-like protein 2-like [Oryz... 159 3e-37
ref|XP_003564024.1| PREDICTED: endonuclease III-like [Brachypodi... 158 8e-37
dbj|BAJ87498.1| predicted protein [Hordeum vulgare subsp. vulgare] 158 8e-37
ref|XP_002438135.1| hypothetical protein SORBIDRAFT_10g008590 [S... 158 8e-37
gb|EMT28319.1| DEMETER-like protein 2 [Aegilops tauschii] 157 1e-36
dbj|BAD37860.1| HhH-GPD base excision DNA repair protein-related... 157 1e-36
ref|NP_001057253.2| Os06g0237900 [Oryza sativa Japonica Group] g... 157 1e-36
gb|EAZ36418.1| hypothetical protein OsJ_20749 [Oryza sativa Japo... 157 1e-36
ref|XP_004965099.1| PREDICTED: protein ROS1-like isoform X2 [Set... 154 2e-35
ref|XP_004965098.1| PREDICTED: protein ROS1-like isoform X1 [Set... 154 2e-35
ref|XP_004508835.1| PREDICTED: protein ROS1-like [Cicer arietinu... 150 1e-34
ref|XP_002321564.2| hypothetical protein POPTR_0015s08260g [Popu... 147 1e-33
ref|XP_003525486.1| PREDICTED: uncharacterized protein LOC100802... 146 3e-33
ref|XP_006404333.1| hypothetical protein EUTSA_v10010580mg [Eutr... 145 4e-33
ref|XP_006439743.1| hypothetical protein CICLE_v10021561mg [Citr... 145 5e-33
ref|NP_566893.1| DNA glycosylase superfamily protein [Arabidopsi... 145 7e-33
>gb|AFW76830.1| hypothetical protein ZEAMMB73_573219 [Zea mays]
Length = 252
Score = 160 bits (406), Expect = 1e-37
Identities = 81/102 (79%), Positives = 91/102 (89%)
Frame = -1
Query: 307 TVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAATKAA 128
TVLDGLV+ LLSQNTTD+ SRRAF SLK+AFP+WDQVVD E K +EDAIRCGGLAATKAA
Sbjct: 138 TVLDGLVTTLLSQNTTDAISRRAFASLKAAFPSWDQVVDEEGKRLEDAIRCGGLAATKAA 197
Query: 127 RIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
RI+S+LR VRE+RGK CLEYLR LSV EVK ELS+FKG+GPK
Sbjct: 198 RIRSMLRDVRERRGKICLEYLRELSVDEVKKELSRFKGIGPK 239
>gb|AFW76829.1| ROS1 protein [Zea mays]
Length = 340
Score = 160 bits (406), Expect = 1e-37
Identities = 81/102 (79%), Positives = 91/102 (89%)
Frame = -1
Query: 307 TVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAATKAA 128
TVLDGLV+ LLSQNTTD+ SRRAF SLK+AFP+WDQVVD E K +EDAIRCGGLAATKAA
Sbjct: 138 TVLDGLVTTLLSQNTTDAISRRAFASLKAAFPSWDQVVDEEGKRLEDAIRCGGLAATKAA 197
Query: 127 RIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
RI+S+LR VRE+RGK CLEYLR LSV EVK ELS+FKG+GPK
Sbjct: 198 RIRSMLRDVRERRGKICLEYLRELSVDEVKKELSRFKGIGPK 239
>gb|AFW76828.1| hypothetical protein ZEAMMB73_573219 [Zea mays]
Length = 264
Score = 160 bits (406), Expect = 1e-37
Identities = 81/102 (79%), Positives = 91/102 (89%)
Frame = -1
Query: 307 TVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAATKAA 128
TVLDGLV+ LLSQNTTD+ SRRAF SLK+AFP+WDQVVD E K +EDAIRCGGLAATKAA
Sbjct: 138 TVLDGLVTTLLSQNTTDAISRRAFASLKAAFPSWDQVVDEEGKRLEDAIRCGGLAATKAA 197
Query: 127 RIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
RI+S+LR VRE+RGK CLEYLR LSV EVK ELS+FKG+GPK
Sbjct: 198 RIRSMLRDVRERRGKICLEYLRELSVDEVKKELSRFKGIGPK 239
>gb|ACG42155.1| ROS1 protein [Zea mays] gi|219887377|gb|ACL54063.1| unknown [Zea
mays]
Length = 276
Score = 160 bits (406), Expect = 1e-37
Identities = 81/102 (79%), Positives = 91/102 (89%)
Frame = -1
Query: 307 TVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAATKAA 128
TVLDGLV+ LLSQNTTD+ SRRAF SLK+AFP+WDQVVD E K +EDAIRCGGLAATKAA
Sbjct: 74 TVLDGLVTTLLSQNTTDAISRRAFASLKAAFPSWDQVVDEEGKRLEDAIRCGGLAATKAA 133
Query: 127 RIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
RI+S+LR VRE+RGK CLEYLR LSV EVK ELS+FKG+GPK
Sbjct: 134 RIRSMLRDVRERRGKICLEYLRELSVDEVKKELSRFKGIGPK 175
>ref|XP_006655955.1| PREDICTED: DEMETER-like protein 2-like [Oryza brachyantha]
Length = 271
Score = 159 bits (403), Expect = 3e-37
Identities = 79/101 (78%), Positives = 90/101 (89%)
Frame = -1
Query: 304 VLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAATKAAR 125
VLDGLV+ LLSQNTTD+ SRRAF SLK+AFPTWDQVVD E K +EDAIRCGGLAATKAAR
Sbjct: 71 VLDGLVTTLLSQNTTDAISRRAFTSLKAAFPTWDQVVDEEGKRLEDAIRCGGLAATKAAR 130
Query: 124 IKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
I+++LR VRE+RG+ CLEYLR LSV EVK ELS+FKG+GPK
Sbjct: 131 IRAMLRGVRERRGRICLEYLRELSVEEVKTELSRFKGIGPK 171
>ref|XP_003564024.1| PREDICTED: endonuclease III-like [Brachypodium distachyon]
Length = 277
Score = 158 bits (399), Expect = 8e-37
Identities = 80/102 (78%), Positives = 90/102 (88%)
Frame = -1
Query: 307 TVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAATKAA 128
TVLDGLV+ LLSQNTTD+ SRRAF SLK+AFP+WDQVVD E K +EDAIRCGGLAATKAA
Sbjct: 76 TVLDGLVTTLLSQNTTDAISRRAFASLKAAFPSWDQVVDEEGKGLEDAIRCGGLAATKAA 135
Query: 127 RIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
RI+++LR VRE+RG CLEYLR LSV EVK ELSQFKG+GPK
Sbjct: 136 RIRAMLRDVRERRGAICLEYLRELSVDEVKRELSQFKGIGPK 177
>dbj|BAJ87498.1| predicted protein [Hordeum vulgare subsp. vulgare]
Length = 282
Score = 158 bits (399), Expect = 8e-37
Identities = 80/102 (78%), Positives = 89/102 (87%)
Frame = -1
Query: 307 TVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAATKAA 128
TVLDGLV+ LLSQNTTD+ SRRAF SLK+AFP+WDQVVD E +EDAIRCGGLAATKAA
Sbjct: 81 TVLDGLVTTLLSQNTTDAISRRAFASLKAAFPSWDQVVDEEGMGLEDAIRCGGLAATKAA 140
Query: 127 RIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
RI+++LR VREKRG CLEYLR LSV EVK ELSQFKG+GPK
Sbjct: 141 RIRAMLRGVREKRGAICLEYLRDLSVDEVKRELSQFKGIGPK 182
>ref|XP_002438135.1| hypothetical protein SORBIDRAFT_10g008590 [Sorghum bicolor]
gi|241916358|gb|EER89502.1| hypothetical protein
SORBIDRAFT_10g008590 [Sorghum bicolor]
Length = 279
Score = 158 bits (399), Expect = 8e-37
Identities = 80/105 (76%), Positives = 90/105 (85%)
Frame = -1
Query: 316 LGETVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAAT 137
L TVLDGLV LLSQNTTD+ SRRAF SLK+AFP+WDQVVD E K +EDAIRCGGLA T
Sbjct: 73 LSPTVLDGLVITLLSQNTTDAISRRAFASLKAAFPSWDQVVDEEGKRLEDAIRCGGLATT 132
Query: 136 KAARIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
KAARI+++LR VRE+RGK CLEYLR LSV EVK ELS+FKG+GPK
Sbjct: 133 KAARIRAMLRDVRERRGKICLEYLRELSVDEVKKELSRFKGIGPK 177
>gb|EMT28319.1| DEMETER-like protein 2 [Aegilops tauschii]
Length = 467
Score = 157 bits (398), Expect = 1e-36
Identities = 80/102 (78%), Positives = 89/102 (87%)
Frame = -1
Query: 307 TVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAATKAA 128
TVLDGLV+ LLSQNTTD+ SRRAF SLK+AFP+WDQVVD E +EDAIRCGGLAATKAA
Sbjct: 75 TVLDGLVTTLLSQNTTDAISRRAFASLKAAFPSWDQVVDEEGMGLEDAIRCGGLAATKAA 134
Query: 127 RIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
RI+++LR VREKRG CLEYLR LSV EVK ELSQFKG+GPK
Sbjct: 135 RIRAMLRGVREKRGAICLEYLRELSVDEVKRELSQFKGIGPK 176
>dbj|BAD37860.1| HhH-GPD base excision DNA repair protein-related-like [Oryza sativa
Japonica Group] gi|51535812|dbj|BAD37897.1| HhH-GPD base
excision DNA repair protein-related-like [Oryza sativa
Japonica Group] gi|218197872|gb|EEC80299.1| hypothetical
protein OsI_22321 [Oryza sativa Indica Group]
Length = 277
Score = 157 bits (397), Expect = 1e-36
Identities = 80/103 (77%), Positives = 91/103 (88%), Gaps = 1/103 (0%)
Frame = -1
Query: 307 TVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEP-KHVEDAIRCGGLAATKA 131
TVLDGLV+ LLSQNTTD+ SRRAF +LK+AFPTWDQVVD E K +EDAIRCGGLAATKA
Sbjct: 75 TVLDGLVTTLLSQNTTDAISRRAFAALKAAFPTWDQVVDEEEGKRLEDAIRCGGLAATKA 134
Query: 130 ARIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
ARI+++LR VRE+RGK CLEYLR LSV EVK ELS+FKG+GPK
Sbjct: 135 ARIRAMLRGVRERRGKICLEYLRDLSVDEVKTELSRFKGIGPK 177
>ref|NP_001057253.2| Os06g0237900 [Oryza sativa Japonica Group]
gi|255676875|dbj|BAF19167.2| Os06g0237900 [Oryza sativa
Japonica Group]
Length = 221
Score = 157 bits (397), Expect = 1e-36
Identities = 80/103 (77%), Positives = 91/103 (88%), Gaps = 1/103 (0%)
Frame = -1
Query: 307 TVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEP-KHVEDAIRCGGLAATKA 131
TVLDGLV+ LLSQNTTD+ SRRAF +LK+AFPTWDQVVD E K +EDAIRCGGLAATKA
Sbjct: 75 TVLDGLVTTLLSQNTTDAISRRAFAALKAAFPTWDQVVDEEEGKRLEDAIRCGGLAATKA 134
Query: 130 ARIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
ARI+++LR VRE+RGK CLEYLR LSV EVK ELS+FKG+GPK
Sbjct: 135 ARIRAMLRGVRERRGKICLEYLRDLSVDEVKTELSRFKGIGPK 177
>gb|EAZ36418.1| hypothetical protein OsJ_20749 [Oryza sativa Japonica Group]
Length = 277
Score = 157 bits (397), Expect = 1e-36
Identities = 80/103 (77%), Positives = 91/103 (88%), Gaps = 1/103 (0%)
Frame = -1
Query: 307 TVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEP-KHVEDAIRCGGLAATKA 131
TVLDGLV+ LLSQNTTD+ SRRAF +LK+AFPTWDQVVD E K +EDAIRCGGLAATKA
Sbjct: 75 TVLDGLVTTLLSQNTTDAISRRAFAALKAAFPTWDQVVDEEEGKRLEDAIRCGGLAATKA 134
Query: 130 ARIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
ARI+++LR VRE+RGK CLEYLR LSV EVK ELS+FKG+GPK
Sbjct: 135 ARIRAMLRGVRERRGKICLEYLRDLSVDEVKTELSRFKGIGPK 177
>ref|XP_004965099.1| PREDICTED: protein ROS1-like isoform X2 [Setaria italica]
Length = 196
Score = 154 bits (388), Expect = 2e-35
Identities = 78/102 (76%), Positives = 88/102 (86%)
Frame = -1
Query: 307 TVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAATKAA 128
TVLDGLV+ LLSQNTT+ SRRAF SLK+AFP+WDQVVD E +EDAIRCGGLAATKAA
Sbjct: 79 TVLDGLVTTLLSQNTTEVISRRAFASLKAAFPSWDQVVDEEGTRLEDAIRCGGLAATKAA 138
Query: 127 RIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
RI+++LR VRE+RGK CLEYLR LSV EVK ELS FKG+GPK
Sbjct: 139 RIRAMLRGVREQRGKICLEYLRELSVDEVKRELSLFKGIGPK 180
>ref|XP_004965098.1| PREDICTED: protein ROS1-like isoform X1 [Setaria italica]
Length = 280
Score = 154 bits (388), Expect = 2e-35
Identities = 78/102 (76%), Positives = 88/102 (86%)
Frame = -1
Query: 307 TVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAATKAA 128
TVLDGLV+ LLSQNTT+ SRRAF SLK+AFP+WDQVVD E +EDAIRCGGLAATKAA
Sbjct: 79 TVLDGLVTTLLSQNTTEVISRRAFASLKAAFPSWDQVVDEEGTRLEDAIRCGGLAATKAA 138
Query: 127 RIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
RI+++LR VRE+RGK CLEYLR LSV EVK ELS FKG+GPK
Sbjct: 139 RIRAMLRGVREQRGKICLEYLRELSVDEVKRELSLFKGIGPK 180
>ref|XP_004508835.1| PREDICTED: protein ROS1-like [Cicer arietinum]
gi|502152248|ref|XP_004508836.1| PREDICTED: protein
ROS1-like [Cicer arietinum]
Length = 285
Score = 150 bits (380), Expect = 1e-34
Identities = 74/103 (71%), Positives = 88/103 (85%)
Frame = -1
Query: 310 ETVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAATKA 131
ETVLDGLV +LSQNTT+SNS +AF SLKS+FPTW+ V AE K +E+AIRCGGLA TKA
Sbjct: 86 ETVLDGLVRTILSQNTTESNSNKAFASLKSSFPTWEHVHGAESKELENAIRCGGLAPTKA 145
Query: 130 ARIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
+ IK++LR + EKRGKFCLEYLR LSVA++KAELS FKG+GPK
Sbjct: 146 SCIKNLLRCLLEKRGKFCLEYLRDLSVAQIKAELSLFKGIGPK 188
>ref|XP_002321564.2| hypothetical protein POPTR_0015s08260g [Populus trichocarpa]
gi|550322300|gb|EEF05691.2| hypothetical protein
POPTR_0015s08260g [Populus trichocarpa]
Length = 306
Score = 147 bits (372), Expect = 1e-33
Identities = 72/103 (69%), Positives = 87/103 (84%)
Frame = -1
Query: 310 ETVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAATKA 131
E+VLDGLV +LSQNTT+ NS+RAFL+LKSAFPTW+ V+ AE K +EDAIRCGGLA TKA
Sbjct: 107 ESVLDGLVKTVLSQNTTEVNSQRAFLNLKSAFPTWENVLAAESKFIEDAIRCGGLAPTKA 166
Query: 130 ARIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
A I++IL ++ EK G+ CLEYLR L VAE+KAELS FKG+GPK
Sbjct: 167 ACIRNILSSLMEKNGRLCLEYLRDLPVAEIKAELSHFKGIGPK 209
>ref|XP_003525486.1| PREDICTED: uncharacterized protein LOC100802952 [Glycine max]
Length = 284
Score = 146 bits (368), Expect = 3e-33
Identities = 72/103 (69%), Positives = 89/103 (86%)
Frame = -1
Query: 310 ETVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAATKA 131
E VLDGLV +LSQNTT++NS++AF SLKS+FP+W+QV+ AE K VE+AIRCGGLA TKA
Sbjct: 83 EPVLDGLVRTVLSQNTTEANSQKAFASLKSSFPSWEQVLWAESKDVENAIRCGGLAPTKA 142
Query: 130 ARIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
+ IK++LR +RE+RG+ CLEYLR LSV EVKAELS FKG+GPK
Sbjct: 143 SCIKNVLRCLRERRGELCLEYLRDLSVDEVKAELSLFKGIGPK 185
>ref|XP_006404333.1| hypothetical protein EUTSA_v10010580mg [Eutrema salsugineum]
gi|557105452|gb|ESQ45786.1| hypothetical protein
EUTSA_v10010580mg [Eutrema salsugineum]
Length = 302
Score = 145 bits (367), Expect = 4e-33
Identities = 71/103 (68%), Positives = 85/103 (82%)
Frame = -1
Query: 310 ETVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAATKA 131
ETVLDGLV +LLSQNTT+ NS+RAF SLK+AFP W+ V+ AEPK +E+AIRCGGLA KA
Sbjct: 100 ETVLDGLVKILLSQNTTEINSQRAFASLKAAFPKWEDVLGAEPKSIENAIRCGGLAPKKA 159
Query: 130 ARIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
IK+IL ++ +RG+ CLEYLRGLSV EVK ELS FKG+GPK
Sbjct: 160 VCIKNILSRLQSERGRLCLEYLRGLSVEEVKTELSHFKGIGPK 202
>ref|XP_006439743.1| hypothetical protein CICLE_v10021561mg [Citrus clementina]
gi|557542005|gb|ESR52983.1| hypothetical protein
CICLE_v10021561mg [Citrus clementina]
Length = 281
Score = 145 bits (366), Expect = 5e-33
Identities = 70/103 (67%), Positives = 87/103 (84%)
Frame = -1
Query: 310 ETVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAATKA 131
E+VLDGLV LLSQNTT++NS +AF SLKS FPTW+ V+ AE K +E+AIRCGGLA TKA
Sbjct: 80 ESVLDGLVKTLLSQNTTEANSLKAFASLKSTFPTWEHVLAAEQKCIENAIRCGGLAPTKA 139
Query: 130 ARIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
A IK+IL+ + E +GK CLEYLRGLS+ E+KAELS+F+G+GPK
Sbjct: 140 ACIKNILKCLLESKGKLCLEYLRGLSIDEIKAELSRFRGIGPK 182
>ref|NP_566893.1| DNA glycosylase superfamily protein [Arabidopsis thaliana]
gi|332644814|gb|AEE78335.1| DNA glycosylase superfamily
protein [Arabidopsis thaliana]
Length = 293
Score = 145 bits (365), Expect = 7e-33
Identities = 71/103 (68%), Positives = 85/103 (82%)
Frame = -1
Query: 310 ETVLDGLVSMLLSQNTTDSNSRRAFLSLKSAFPTWDQVVDAEPKHVEDAIRCGGLAATKA 131
E+VLDGLV +LLSQNTT+SNS+RAF SLK+ FP WD V++AE K +E+AIRCGGLA KA
Sbjct: 94 ESVLDGLVKILLSQNTTESNSQRAFASLKATFPKWDDVLNAESKSIENAIRCGGLAPKKA 153
Query: 130 ARIKSILRAVREKRGKFCLEYLRGLSVAEVKAELSQFKGVGPK 2
IK+IL ++ +RG+ CLEYLRGLSV EVK ELS FKGVGPK
Sbjct: 154 VCIKNILNRLQNERGRLCLEYLRGLSVEEVKTELSHFKGVGPK 196