BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Stemona21_contig00029708
         (436 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002467741.1| hypothetical protein SORBIDRAFT_01g033250 [S...    75   9e-12
gb|EXC32853.1| Trimethylguanosine synthase [Morus notabilis]           71   1e-10
dbj|BAJ90926.1| predicted protein [Hordeum vulgare subsp. vulgare]     70   2e-10
ref|XP_004984106.1| PREDICTED: trimethylguanosine synthase-like ...    70   4e-10
ref|XP_004984105.1| PREDICTED: trimethylguanosine synthase-like ...    70   4e-10
gb|EMS61538.1| Trimethylguanosine synthase [Triticum urartu]           70   4e-10
ref|NP_001144779.1| uncharacterized protein LOC100277845 [Zea ma...    68   1e-09
gb|EOY14435.1| S-adenosyl-L-methionine-dependent methyltransfera...    68   1e-09
gb|EMT01536.1| Trimethylguanosine synthase [Aegilops tauschii]         66   5e-09
ref|XP_004291100.1| PREDICTED: uncharacterized protein LOC101302...    66   5e-09
emb|CBI19510.3| unnamed protein product [Vitis vinifera]               65   7e-09
ref|XP_002282801.1| PREDICTED: uncharacterized protein LOC100262...    65   7e-09
gb|EAY90339.1| hypothetical protein OsI_11916 [Oryza sativa Indi...    65   7e-09
ref|NP_001050299.1| Os03g0396900 [Oryza sativa Japonica Group] g...    64   3e-08
ref|XP_006651475.1| PREDICTED: uncharacterized protein LOC102708...    62   8e-08
ref|XP_004141260.1| PREDICTED: uncharacterized protein LOC101215...    62   8e-08
ref|XP_006409319.1| hypothetical protein EUTSA_v10022632mg [Eutr...    61   2e-07
ref|XP_004499054.1| PREDICTED: uncharacterized protein LOC101505...    59   5e-07
ref|XP_006342628.1| PREDICTED: uncharacterized protein LOC102606...    58   1e-06
gb|ESW32852.1| hypothetical protein PHAVU_001G022600g [Phaseolus...    55   7e-06

>ref|XP_002467741.1| hypothetical protein SORBIDRAFT_01g033250 [Sorghum bicolor]
           gi|241921595|gb|EER94739.1| hypothetical protein
           SORBIDRAFT_01g033250 [Sorghum bicolor]
          Length = 690

 Score = 75.1 bits (183), Expect = 9e-12
 Identities = 48/111 (43%), Positives = 65/111 (58%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDYAFV 243
           LG LF++TEV+LWDD+     P   R++    EA  V  + T  +   + N     + FV
Sbjct: 14  LGQLFRLTEVYLWDDSY-GAGPHDGRKNWRSAEAALVD-SHTDKTCNEASNGTDKGHLFV 71

Query: 244 EDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSADKDSENRA 396
           EDLELA  MG+LGLP+SFST++  K+ G+ GK+KG R   L A K   N A
Sbjct: 72  EDLELANLMGSLGLPVSFSTSKVNKNTGNKGKKKG-RQALLKAAKTQINDA 121


>gb|EXC32853.1| Trimethylguanosine synthase [Morus notabilis]
          Length = 733

 Score = 71.2 bits (173), Expect = 1e-10
 Identities = 47/119 (39%), Positives = 61/119 (51%), Gaps = 8/119 (6%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDYAFV 243
           LGSLF++TE+ LWDD   +       RD    +A    I       +   NDK SD  F 
Sbjct: 16  LGSLFRLTEIHLWDDGSAE------ARDRSSFQADTARIKAAQEGDDD--NDKDSDVHFT 67

Query: 244 --------EDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSADKDSENRA 396
                   ED EL +QM ALGLP+SF+T +EK+   + GKRKGMR K     +D+   A
Sbjct: 68  STDANVLSEDEELTRQMNALGLPLSFNTNKEKRSGKTKGKRKGMRQKHTDTCQDTFGEA 126


>dbj|BAJ90926.1| predicted protein [Hordeum vulgare subsp. vulgare]
          Length = 690

 Score = 70.5 bits (171), Expect = 2e-10
 Identities = 41/109 (37%), Positives = 59/109 (54%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDYAFV 243
           LG LF++TEV LWDD  V         D    E  +   + T+ +   +      D++FV
Sbjct: 62  LGRLFRLTEVHLWDDFYVK------PHDWRATETTDCTGSQTAKTRNKAAKQTDEDHSFV 115

Query: 244 EDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSADKDSEN 390
           ED+ELA  MG+LGLP+SFST +EKK   + GK +G +    +A    +N
Sbjct: 116 EDMELASLMGSLGLPVSFSTRKEKKKTPAKGKHQGRQAPYEAATPMDDN 164


>ref|XP_004984106.1| PREDICTED: trimethylguanosine synthase-like isoform X2 [Setaria
           italica]
          Length = 661

 Score = 69.7 bits (169), Expect = 4e-10
 Identities = 40/112 (35%), Positives = 59/112 (52%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDYAFV 243
           LG LF +TEV LWDD+    + A   +  W                  +  D    ++FV
Sbjct: 14  LGRLFWITEVHLWDDSYA--AGASDGQKHWRSAEAAPADFHADKPCNKASKDTDKGHSFV 71

Query: 244 EDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSADKDSENRAR 399
           EDLELA  MG+LGLP+SFST++  K+ G+ GK+KG +V+  +     ++  R
Sbjct: 72  EDLELANLMGSLGLPVSFSTSKANKNTGNKGKKKGRQVQCEAGSTQIDDAVR 123


>ref|XP_004984105.1| PREDICTED: trimethylguanosine synthase-like isoform X1 [Setaria
           italica]
          Length = 675

 Score = 69.7 bits (169), Expect = 4e-10
 Identities = 40/112 (35%), Positives = 59/112 (52%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDYAFV 243
           LG LF +TEV LWDD+    + A   +  W                  +  D    ++FV
Sbjct: 14  LGRLFWITEVHLWDDSYA--AGASDGQKHWRSAEAAPADFHADKPCNKASKDTDKGHSFV 71

Query: 244 EDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSADKDSENRAR 399
           EDLELA  MG+LGLP+SFST++  K+ G+ GK+KG +V+  +     ++  R
Sbjct: 72  EDLELANLMGSLGLPVSFSTSKANKNTGNKGKKKGRQVQCEAGSTQIDDAVR 123


>gb|EMS61538.1| Trimethylguanosine synthase [Triticum urartu]
          Length = 677

 Score = 69.7 bits (169), Expect = 4e-10
 Identities = 41/112 (36%), Positives = 59/112 (52%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDYAFV 243
           LG LF++TEV LWDD  V         D    E      + T+ +   +      D++FV
Sbjct: 15  LGRLFRLTEVHLWDDFYVK------PHDWRATETVGCTGSQTAKTRNKAAKQTDEDHSFV 68

Query: 244 EDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSADKDSENRAR 399
           ED+ELA  MG+LGLP+SFST +EKK   + GK +G +    +A    ++  R
Sbjct: 69  EDMELASLMGSLGLPVSFSTRKEKKKTPAKGKHQGRQAPYEAARTPMDDNVR 120


>ref|NP_001144779.1| uncharacterized protein LOC100277845 [Zea mays]
           gi|195646870|gb|ACG42903.1| hypothetical protein [Zea
           mays] gi|414867143|tpg|DAA45700.1| TPA: hypothetical
           protein ZEAMMB73_369989 [Zea mays]
          Length = 476

 Score = 68.2 bits (165), Expect = 1e-09
 Identities = 41/112 (36%), Positives = 63/112 (56%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDYAFV 243
           LG LF++TEV+LWDD+     P   +++    EA  V  + T  +   + N     + FV
Sbjct: 14  LGKLFRLTEVYLWDDSY-GAGPHDGQKNGRSAEAALVD-SHTDKTCNEASNSTDKGHFFV 71

Query: 244 EDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSADKDSENRAR 399
           EDLEL   MG+LGLP+SFST++  K+  + GK+KG +    +A+    +  R
Sbjct: 72  EDLELVNLMGSLGLPVSFSTSKVNKNTCNKGKKKGRQAPLKAANTQINDAVR 123


>gb|EOY14435.1| S-adenosyl-L-methionine-dependent methyltransferases superfamily
           protein, putative isoform 1 [Theobroma cacao]
          Length = 652

 Score = 67.8 bits (164), Expect = 1e-09
 Identities = 43/109 (39%), Positives = 60/109 (55%), Gaps = 1/109 (0%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDYAFV 243
           LG LFK+TEV+LWDD   +      R+     E++N   +        SV    SD   +
Sbjct: 16  LGFLFKLTEVYLWDDGAKE-----TRQSSCLCESENNKSSRQRDKSRSSVFHMTSDSCTL 70

Query: 244 -EDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSADKDSE 387
            ED+ELA +M ALGLP+SF T++E +   +  KRKG+R K     KD+E
Sbjct: 71  PEDIELANEMNALGLPLSFHTSKETRSKMTSSKRKGVRSKHSHGHKDTE 119


>gb|EMT01536.1| Trimethylguanosine synthase [Aegilops tauschii]
          Length = 763

 Score = 65.9 bits (159), Expect = 5e-09
 Identities = 39/113 (34%), Positives = 58/113 (51%), Gaps = 1/113 (0%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCI-TDTSSSVEPSVNDKHSDYAF 240
           LG L ++TEV LWDD         A+   W       C  + T+ +   +      D++F
Sbjct: 90  LGRLLRLTEVHLWDDFY-------AKPHDWRATDTAGCTGSQTAKTRNKAAKQTDEDHSF 142

Query: 241 VEDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSADKDSENRAR 399
           VED+ELA  MG+LGLP+SF T +EKK   + GK +G +    +A    ++  R
Sbjct: 143 VEDMELASLMGSLGLPVSFITRKEKKKTPAKGKHQGRQASYEAASTPMDDNVR 195


>ref|XP_004291100.1| PREDICTED: uncharacterized protein LOC101302516 [Fragaria vesca
           subsp. vesca]
          Length = 770

 Score = 65.9 bits (159), Expect = 5e-09
 Identities = 40/103 (38%), Positives = 57/103 (55%), Gaps = 4/103 (3%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDYA-- 237
           LGSL K+TEVFLWDD          R D+      +  +  T  + +   +D +   +  
Sbjct: 14  LGSLLKLTEVFLWDDGT--------RNDM------SFSLKQTKPAQDDGDDDSNGLASKG 59

Query: 238 --FVEDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVK 360
               ED+EL +QM  LGLP+SF T ++K+   +GGKRKGMR+K
Sbjct: 60  GILPEDMELTRQMNELGLPVSFQTNKQKQKRKAGGKRKGMRLK 102


>emb|CBI19510.3| unnamed protein product [Vitis vinifera]
          Length = 683

 Score = 65.5 bits (158), Expect = 7e-09
 Identities = 42/116 (36%), Positives = 63/116 (54%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDYAFV 243
           LGSLF++T+V LWDDA  +  P ++        +      + +S +  + ND +    +V
Sbjct: 11  LGSLFRLTQVSLWDDASAEV-PEVSSFSRRSKPSAGDEEDEGNSDIISAANDYNPSQEYV 69

Query: 244 EDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSADKDSENRARGLPK 411
           E   LA++M ALGLPISF T +EK++    GKRK  ++K    DK  E+     PK
Sbjct: 70  E---LAKEMNALGLPISFHTNKEKRNAVVKGKRKSKQMKHPHCDKQIEDGVLEFPK 122


>ref|XP_002282801.1| PREDICTED: uncharacterized protein LOC100262697 [Vitis vinifera]
          Length = 780

 Score = 65.5 bits (158), Expect = 7e-09
 Identities = 42/116 (36%), Positives = 63/116 (54%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDYAFV 243
           LGSLF++T+V LWDDA  +  P ++        +      + +S +  + ND +    +V
Sbjct: 11  LGSLFRLTQVSLWDDASAEV-PEVSSFSRRSKPSAGDEEDEGNSDIISAANDYNPSQEYV 69

Query: 244 EDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSADKDSENRARGLPK 411
           E   LA++M ALGLPISF T +EK++    GKRK  ++K    DK  E+     PK
Sbjct: 70  E---LAKEMNALGLPISFHTNKEKRNAVVKGKRKSKQMKHPHCDKQIEDGVLEFPK 122


>gb|EAY90339.1| hypothetical protein OsI_11916 [Oryza sativa Indica Group]
          Length = 690

 Score = 65.5 bits (158), Expect = 7e-09
 Identities = 43/107 (40%), Positives = 62/107 (57%), Gaps = 3/107 (2%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDD---AVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDY 234
           LG  F++TE  +WD    A  D S    R D  D+++   C TD   + +P+      D 
Sbjct: 15  LGRHFRLTEAHIWDGWYAAGADVSHRSWRSD--DIDSGGCCQTDKVQN-KPTKQTDEGDL 71

Query: 235 AFVEDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSAD 375
            FVEDLEL+  MG+LGLP+SFST++EKK+  +  K+ G RV   +A+
Sbjct: 72  -FVEDLELSNLMGSLGLPVSFSTSKEKKNAPNKVKKNGRRVSYEAAN 117


>ref|NP_001050299.1| Os03g0396900 [Oryza sativa Japonica Group]
           gi|14029021|gb|AAK52562.1|AC079853_15 Unknown protein
           [Oryza sativa Japonica Group]
           gi|108708634|gb|ABF96429.1| WW domain-containing
           protein, putative, expressed [Oryza sativa Japonica
           Group] gi|113548770|dbj|BAF12213.1| Os03g0396900 [Oryza
           sativa Japonica Group] gi|215737194|dbj|BAG96123.1|
           unnamed protein product [Oryza sativa Japonica Group]
           gi|222625074|gb|EEE59206.1| hypothetical protein
           OsJ_11156 [Oryza sativa Japonica Group]
          Length = 690

 Score = 63.5 bits (153), Expect = 3e-08
 Identities = 42/107 (39%), Positives = 61/107 (57%), Gaps = 3/107 (2%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDD---AVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDY 234
           LG  F++TE  +WD    A  D S      D  D+++   C TD   + +P+      D 
Sbjct: 15  LGRHFRLTEAHIWDGWYAAGADVSHRSWHSD--DIDSGGCCQTDKVQN-KPTKQTDEGDL 71

Query: 235 AFVEDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSAD 375
            FVEDLEL+  MG+LGLP+SFST++EKK+  +  K+ G RV   +A+
Sbjct: 72  -FVEDLELSNLMGSLGLPVSFSTSKEKKNAPNKVKKNGRRVSYEAAN 117


>ref|XP_006651475.1| PREDICTED: uncharacterized protein LOC102708232 [Oryza brachyantha]
          Length = 685

 Score = 62.0 bits (149), Expect = 8e-08
 Identities = 37/99 (37%), Positives = 58/99 (58%), Gaps = 1/99 (1%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNV-CITDTSSSVEPSVNDKHSDYAF 240
           LG  F++TEV +WDD   D +  ++ R    ++  +  C TD   +      D+   ++F
Sbjct: 15  LGRHFRLTEVHIWDDWYADGAD-VSHRSWRSVDTDSAGCQTDKIQNKPAKQTDE--GHSF 71

Query: 241 VEDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRV 357
           VEDLEL   MG+LGLP+SF T++EKK+  +  K+ G +V
Sbjct: 72  VEDLELVNLMGSLGLPVSFRTSKEKKNTPNKVKKNGRKV 110


>ref|XP_004141260.1| PREDICTED: uncharacterized protein LOC101215034 [Cucumis sativus]
           gi|449519340|ref|XP_004166693.1| PREDICTED:
           uncharacterized protein LOC101228946 [Cucumis sativus]
          Length = 789

 Score = 62.0 bits (149), Expect = 8e-08
 Identities = 43/119 (36%), Positives = 61/119 (51%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDYAFV 243
           LGSLFK+TEVFLWD+        +ARR +    A +    +   SVE   +         
Sbjct: 20  LGSLFKLTEVFLWDEET-----EVARR-VESRLALDADDANNGKSVEKICSTISGISLLP 73

Query: 244 EDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSADKDSENRARGLPKAGA 420
           ED+EL +QM ALGLP+SF T +E+K IG    ++   VK     +   ++    PKA +
Sbjct: 74  EDIELTEQMNALGLPLSFHTNKEQKRIGITMVKRKANVKHSRIQQGFLDKEVEFPKASS 132


>ref|XP_006409319.1| hypothetical protein EUTSA_v10022632mg [Eutrema salsugineum]
           gi|557110481|gb|ESQ50772.1| hypothetical protein
           EUTSA_v10022632mg [Eutrema salsugineum]
          Length = 544

 Score = 60.8 bits (146), Expect = 2e-07
 Identities = 38/106 (35%), Positives = 58/106 (54%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDYAFV 243
           LGSLFK+T++ LWDD   D        +  D+   N  I DTS+ +    N +       
Sbjct: 16  LGSLFKLTQIHLWDDGSTDTHLVPLFYEHSDV-FPNKSIDDTSNKLSGIANHRS------ 68

Query: 244 EDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSADKD 381
           ED+ L ++M ALGLP+SF T +E K    G ++KG++  +L  ++D
Sbjct: 69  EDMGLIKEMKALGLPVSFQTNKEWKTRTRGHQKKGIKFNTLLNEED 114


>ref|XP_004499054.1| PREDICTED: uncharacterized protein LOC101505223 [Cicer arietinum]
          Length = 708

 Score = 59.3 bits (142), Expect = 5e-07
 Identities = 41/105 (39%), Positives = 56/105 (53%), Gaps = 6/105 (5%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKH-----S 228
           LGSLFKVT+VFLWDD              + L+      +D S + + + +D +     S
Sbjct: 13  LGSLFKVTQVFLWDDG-------------FQLQPSTQLCSDLSEA-DHNYHDSNPVPDWS 58

Query: 229 DYAFV-EDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVK 360
           +   + EDLEL +QM  LGLP++F T+  KK     GKRKG R K
Sbjct: 59  ELVIIPEDLELTKQMNELGLPLTFHTSNNKKCGPVKGKRKGARSK 103


>ref|XP_006342628.1| PREDICTED: uncharacterized protein LOC102606264 isoform X2 [Solanum
           tuberosum]
          Length = 665

 Score = 57.8 bits (138), Expect = 1e-06
 Identities = 42/103 (40%), Positives = 57/103 (55%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDYAFV 243
           LGSLFK+TEV L D          ++     LE+ N   TD  +S    +N      +  
Sbjct: 20  LGSLFKLTEVHLSD---------YSQEGKLLLESAN---TDVYNSNASPLNVLAEFGSLP 67

Query: 244 EDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSA 372
           EDLELA+QM  +GLP+SF T +EK++    GKRK  + KSLS+
Sbjct: 68  EDLELARQMNEMGLPVSFHTNKEKRNRTVKGKRKDGKKKSLSS 110


>gb|ESW32852.1| hypothetical protein PHAVU_001G022600g [Phaseolus vulgaris]
          Length = 713

 Score = 55.5 bits (132), Expect = 7e-06
 Identities = 40/107 (37%), Positives = 54/107 (50%)
 Frame = +1

Query: 64  LGSLFKVTEVFLWDDAVVDFSPALARRDIWDLEAKNVCITDTSSSVEPSVNDKHSDYAFV 243
           LGSLFKVT+VFLWD  +   S          +E  N+     S  V    +++ +     
Sbjct: 16  LGSLFKVTQVFLWDYGIQVSS---------SVEQLNLAENHGSDPV----HEEFTGLITP 62

Query: 244 EDLELAQQMGALGLPISFSTTREKKDIGSGGKRKGMRVKSLSADKDS 384
           EDLEL +Q+  LGLP+SF T +EKK     G  KG +   LS   D+
Sbjct: 63  EDLELNKQLNELGLPLSFQTNKEKK-----GPLKGKKKGKLSKHPDT 104