BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Scutellaria22_contig00032565
         (744 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAP68990.1| 4-coumarate:coenzyme A ligase 1 [Salvia miltiorrh...   360   1e-97
gb|AAF91309.1|AF239686_1 4-coumarate:coA ligase 2 [Rubus idaeus]      277   2e-72
ref|XP_002533186.1| AMP dependent CoA ligase, putative [Ricinus ...   268   1e-69
sp|O24540.1|4CL_VANPL RecName: Full=4-coumarate--CoA ligase; Sho...   262   5e-68
ref|XP_002329649.1| 4-Coumarate:CoA ligase [Populus trichocarpa]...   262   6e-68

>gb|AAP68990.1| 4-coumarate:coenzyme A ligase 1 [Salvia miltiorrhiza]
          Length = 535

 Score =  360 bits (925), Expect = 1e-97
 Identities = 174/234 (74%), Positives = 198/234 (84%), Gaps = 4/234 (1%)
 Frame = +1

Query: 55  MELKHETEHIFRSKLPDIYIPNHLPIHTYCFQNISTHCSRPFLINAATGEVFTHADTELA 234
           MELK E+EHIFRSKLPDI IP HLP+HTYCFQN+STH +RP+LINAATG+ FTHA  EL 
Sbjct: 1   MELKQESEHIFRSKLPDINIPTHLPLHTYCFQNLSTHRARPYLINAATGDTFTHAGFELT 60

Query: 235 SRRVAVGLHNLCIRKSDVIMLLLHNSPEFAFSFLAASFIGATSTTANPHYTAAEIMLQVG 414
           +RRVA GLHNL IRKSDV+ LLLHNSPEFAF+FL ASFIGA STTANP YTA+EI LQ  
Sbjct: 61  ARRVAAGLHNLGIRKSDVVTLLLHNSPEFAFAFLGASFIGAISTTANPLYTASEIALQAR 120

Query: 415 VSKPKLIITHACHVDKVKH----CGAIIVSVDPPPSPQIIHFKELMKSDETLLPKVEIYA 582
           +S+PKLI+THACHV+KVKH     GA I ++DPPPSP+IIHF EL +SDE LL  +EI+A
Sbjct: 121 ISRPKLIVTHACHVEKVKHYAAEAGAKIATIDPPPSPEIIHFAELRRSDEKLLTPIEIHA 180

Query: 583 DDTAVLPFSSGTTGLPKGVMLSHKNLVTCVSQQIDGENPANHTDSDDLLICVLP 744
           DDT  LPFSSGTTGLPKGVMLSHKNLV CVSQQ+DGENPA H D +D ++CVLP
Sbjct: 181 DDTVALPFSSGTTGLPKGVMLSHKNLVACVSQQVDGENPAVHIDREDRMLCVLP 234


>gb|AAF91309.1|AF239686_1 4-coumarate:coA ligase 2 [Rubus idaeus]
          Length = 544

 Score =  277 bits (708), Expect = 2e-72
 Identities = 140/236 (59%), Positives = 172/236 (72%), Gaps = 6/236 (2%)
 Frame = +1

Query: 55  MELKHETEH--IFRSKLPDIYIPNHLPIHTYCFQNISTHCSRPFLINAATGEVFTHADTE 228
           ME KH+ +H  IFRSKLPDIYIPNHLP+HTYCF+NIS    RP LIN  TGE FT+A+ E
Sbjct: 1   MENKHQDDHEFIFRSKLPDIYIPNHLPLHTYCFENISQFHDRPCLINGNTGETFTYAEVE 60

Query: 229 LASRRVAVGLHNLCIRKSDVIMLLLHNSPEFAFSFLAASFIGATSTTANPHYTAAEIMLQ 408
           L SRRVA GL  L I+++DV+MLLL N PEFAF+FL AS+IGA STTANP YT AE+  Q
Sbjct: 61  LTSRRVAAGLDKLGIQQNDVVMLLLQNCPEFAFAFLGASYIGAMSTTANPFYTPAEVAKQ 120

Query: 409 VGVSKPKLIITHACHVDKVKHCGAI----IVSVDPPPSPQIIHFKELMKSDETLLPKVEI 576
              S  KLIIT + +VDKVK    +    ++ VD   S  ++HF ELM +DE+  P V+I
Sbjct: 121 AKASNAKLIITQSAYVDKVKDFAKLNDVKVMCVDETSSEDVLHFSELMSADESETPAVKI 180

Query: 577 YADDTAVLPFSSGTTGLPKGVMLSHKNLVTCVSQQIDGENPANHTDSDDLLICVLP 744
             DD   LP+SSGTTGLPKGVML+HK LVT V+QQ+DGENP  +   +D+++CVLP
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLP 236


>ref|XP_002533186.1| AMP dependent CoA ligase, putative [Ricinus communis]
           gi|223527010|gb|EEF29200.1| AMP dependent CoA ligase,
           putative [Ricinus communis]
          Length = 557

 Score =  268 bits (684), Expect = 1e-69
 Identities = 136/235 (57%), Positives = 168/235 (71%), Gaps = 9/235 (3%)
 Frame = +1

Query: 67  HETEHIFRSKLPDIYIPNHLPIHTYCFQNISTHCSRPFLINAATGEVFTHADTELASRRV 246
           H  E IFRSKLPDIYIPNHLP+HTYCF+NIS       LIN ATG+V+T+AD EL SR+V
Sbjct: 15  HHQEFIFRSKLPDIYIPNHLPLHTYCFENISQFKDNACLINGATGDVYTYADVELTSRKV 74

Query: 247 AVGLHNLCIRKSDVIMLLLHNSPEFAFSFLAASFIGATSTTANPHYTAAEIMLQVGVSKP 426
           A GLH L I++++VIM+LL NSPEF F+FL AS +GA STTANP YT AEI  Q   S  
Sbjct: 75  AAGLHKLGIKQNEVIMILLQNSPEFVFAFLGASVLGAISTTANPFYTPAEIKKQASASNA 134

Query: 427 KLIITHACHVDKVK----HCGAIIVSVDPPPSP-----QIIHFKELMKSDETLLPKVEIY 579
           KLIIT A +V+KVK      G  I+++D PP         +HF EL K+DE  +P V+I 
Sbjct: 135 KLIITQAAYVEKVKDFANENGIKIMTIDSPPPALDDCRDCLHFSELTKADENDIPAVKIN 194

Query: 580 ADDTAVLPFSSGTTGLPKGVMLSHKNLVTCVSQQIDGENPANHTDSDDLLICVLP 744
            DD   LP+SSGTTGLPKGVML+HK+LVT V+QQ+DGENP  +    D+++C+LP
Sbjct: 195 PDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFHEKDVILCLLP 249


>sp|O24540.1|4CL_VANPL RecName: Full=4-coumarate--CoA ligase; Short=4CL; AltName:
           Full=4-coumaroyl-CoA synthase
          Length = 553

 Score =  262 bits (670), Expect = 5e-68
 Identities = 135/229 (58%), Positives = 164/229 (71%), Gaps = 8/229 (3%)
 Frame = +1

Query: 82  IFRSKLPDIYIPNHLPIHTYCFQNISTHCSRPFLINAATGEVFTHADTELASRRVAVGLH 261
           IFRSKLPDIYIP +LP+H+YCF+NIS   SRP LIN AT E+FT+AD EL SRRV  GL 
Sbjct: 15  IFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELISRRVGSGLS 74

Query: 262 NLCIRKSDVIMLLLHNSPEFAFSFLAASFIGATSTTANPHYTAAEIMLQVGVSKPKLIIT 441
            L I++ D IM+LL NSPEF F+FL ASFIG+ ST ANP +T+ E++ Q   S  KLIIT
Sbjct: 75  KLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQAKASNAKLIIT 134

Query: 442 HACHVDKVK----HCGAIIVSVD----PPPSPQIIHFKELMKSDETLLPKVEIYADDTAV 597
             C+VDKVK      G  I+S+D       +  I+HF EL  +DE  +PKVEI  D    
Sbjct: 135 QGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEMPKVEISPDGVVA 194

Query: 598 LPFSSGTTGLPKGVMLSHKNLVTCVSQQIDGENPANHTDSDDLLICVLP 744
           LP+SSGTTGLPKGVML+HK LVT V+QQ+DGENP  +  SDD+L+CVLP
Sbjct: 195 LPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLP 243


>ref|XP_002329649.1| 4-Coumarate:CoA ligase [Populus trichocarpa]
           gi|222870530|gb|EEF07661.1| 4-Coumarate:CoA ligase
           [Populus trichocarpa]
          Length = 545

 Score =  262 bits (669), Expect = 6e-68
 Identities = 137/236 (58%), Positives = 165/236 (69%), Gaps = 5/236 (2%)
 Frame = +1

Query: 52  EMELKHETEHIFRSKLPDIYIPNHLPIHTYCFQNISTHCSRPFLINAATGEVFTHADTEL 231
           E E     E IFRSKLPDI+IPNHLP+HTYCF+N+S     P LIN  TGE+ T+AD EL
Sbjct: 2   EAENDQAQEFIFRSKLPDIHIPNHLPLHTYCFENLSRFKDNPCLINGPTGEIHTYADVEL 61

Query: 232 ASRRVAVGLHNLCIRKSDVIMLLLHNSPEFAFSFLAASFIGATSTTANPHYTAAEIMLQV 411
            SR+VA GL+ L I++ DVI+LLL NSPEF F+FL AS IGA STTANP YT AE+  Q 
Sbjct: 62  TSRKVASGLNKLGIKQGDVILLLLQNSPEFVFAFLGASIIGAISTTANPFYTPAEVAKQA 121

Query: 412 GVSKPKLIITHACHVDKV-----KHCGAIIVSVDPPPSPQIIHFKELMKSDETLLPKVEI 576
             SK KLIIT A + +KV     ++    IV+VD PP    +HF EL  SDE  +P VEI
Sbjct: 122 TASKAKLIITQAVYAEKVQQFVKENDHVKIVTVDSPPE-NYLHFSELTNSDEDDIPAVEI 180

Query: 577 YADDTAVLPFSSGTTGLPKGVMLSHKNLVTCVSQQIDGENPANHTDSDDLLICVLP 744
             DD   LP+SSGTTGLPKGVML+HK LVT V+QQ+DGENP  +    D+++CVLP
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHEKDVILCVLP 236