BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Salvia21_contig00002101
         (2097 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002282577.1| PREDICTED: calcium/calmodulin-dependent seri...   912   0.0  
ref|XP_002322616.1| predicted protein [Populus trichocarpa] gi|2...   909   0.0  
emb|CBI32784.3| unnamed protein product [Vitis vinifera]              907   0.0  
ref|XP_004141122.1| PREDICTED: calcium/calmodulin-dependent seri...   900   0.0  
ref|XP_003518984.1| PREDICTED: calcium/calmodulin-dependent seri...   892   0.0  

>ref|XP_002282577.1| PREDICTED: calcium/calmodulin-dependent serine/threonine-protein
            kinase 1 [Vitis vinifera]
          Length = 575

 Score =  912 bits (2356), Expect = 0.0
 Identities = 464/576 (80%), Positives = 503/576 (87%)
 Frame = -1

Query: 2097 LCHGKPIETPDNLSGNSINPGETNELTLNSTGKTXXXXXXXXXXXXXXXKNSPANSSSVT 1918
            LC GKPIE P   S + I PG+    T   T KT               KNSPANSS V+
Sbjct: 3    LCQGKPIENPQTQSQDLIIPGDGELSTNTQTTKTPKFPFYSPSPLPSGFKNSPANSS-VS 61

Query: 1917 STXXXXXXXXXXXXXPAKHIKALLARRHGSVKPNEATIPEGSECEIVGLDKNFGYSKSFT 1738
            ST             PAKHI+ALLARRHGSVKPNEATIPEG+ECE VGLDKNFG+SK F 
Sbjct: 62   STPLRIFKRPFPPPSPAKHIRALLARRHGSVKPNEATIPEGNECE-VGLDKNFGFSKQFV 120

Query: 1737 SHYEIGEEVGRGHFGYTCSAKGKKGSLRGQDVAVKVIPKSKMTTAIAIEDVRREVKILRA 1558
            +HYE+GEEVGRGHFGYT SAK KKGSL+GQDVAVKVI KSKMTTAIAIEDVRREVKILRA
Sbjct: 121  AHYEMGEEVGRGHFGYTSSAKAKKGSLKGQDVAVKVIAKSKMTTAIAIEDVRREVKILRA 180

Query: 1557 LTGHKNLVQFYEAYEDEENVYVVMELCNGGELLDRILSRGGKYAEEDAKVVMVQILNVVA 1378
            LTGHKNLVQFYEAYED++NVY+VMELC GGELLDRIL+RGGKY+EEDAK VMVQILNV A
Sbjct: 181  LTGHKNLVQFYEAYEDDDNVYIVMELCRGGELLDRILARGGKYSEEDAKAVMVQILNVTA 240

Query: 1377 YCHLQGVVHRDLKPENFLFVSKDEHSSLKAIDFGLSDYVKPDERLNDIVGSAYYVAPEVL 1198
            +CHLQGVVHRDLKPENFLF+SK+E+S LKAIDFGLSDYVKPDERLNDIVGSAYYVAPEVL
Sbjct: 241  FCHLQGVVHRDLKPENFLFISKEENSPLKAIDFGLSDYVKPDERLNDIVGSAYYVAPEVL 300

Query: 1197 HRSYGTEADMWSIGVIAYILLCGSRPFWARTESGIFRAVLKADPSFEEDPWPNLSPDAID 1018
            HRSYGTEADMWS+GVIAYILLCGSRPFWARTESGIF+AVLKADPSF+E PWP+LS DAID
Sbjct: 301  HRSYGTEADMWSVGVIAYILLCGSRPFWARTESGIFKAVLKADPSFDEAPWPSLSSDAID 360

Query: 1017 FVKQLLNKDYRKRLTAAQALSHPWLAGHHDVKIPLDMIMYRLVKAYVCSSSLRKAALGAL 838
            FVK+LLNKDYRKRLTAAQALSHPWLA +HDVKIPLDMI+Y+LVKAY+ SSSLRK+AL AL
Sbjct: 361  FVKRLLNKDYRKRLTAAQALSHPWLANYHDVKIPLDMIVYKLVKAYIFSSSLRKSALAAL 420

Query: 837  ARTLTIPQLAYLREQFALLGPSKSGFISLQNFKTAMVKNSTDAVKDSRVLEYVNVVSSLQ 658
            A+TL+I QLAY REQF LLGP+KSGF+SLQNFKTA+ KNSTDA+KDSRVLEYV++V SLQ
Sbjct: 421  AKTLSIAQLAYFREQFTLLGPNKSGFVSLQNFKTAVTKNSTDAIKDSRVLEYVSMVGSLQ 480

Query: 657  YRKFDFEEFCAAAVSVHQLEGIASWEQHARSAYELFEKDGNRPIMIEELASELGLSPSVP 478
            YRK DFEEFCAAA+SV QLEG+ SWEQHAR  YELFEKDGNRPIMIEELASELGLSPSVP
Sbjct: 481  YRKLDFEEFCAAAISVLQLEGMESWEQHARRGYELFEKDGNRPIMIEELASELGLSPSVP 540

Query: 477  VHVVLQDWIRHSDGKLSFLGFVRLLHGVSSRSFQKA 370
            VHVVLQDWIRHSDGKLSFLGFVRLLHG  SR+F KA
Sbjct: 541  VHVVLQDWIRHSDGKLSFLGFVRLLHG-PSRAFPKA 575


>ref|XP_002322616.1| predicted protein [Populus trichocarpa] gi|222867246|gb|EEF04377.1|
            predicted protein [Populus trichocarpa]
          Length = 578

 Score =  909 bits (2350), Expect = 0.0
 Identities = 466/578 (80%), Positives = 507/578 (87%), Gaps = 2/578 (0%)
 Frame = -1

Query: 2097 LCHGKPIETPDNLSGNSINPGETNELT-LNS-TGKTXXXXXXXXXXXXXXXKNSPANSSS 1924
            LCHGKPIE   N S N+    ET+     NS T KT               K SPA  SS
Sbjct: 3    LCHGKPIELQKNQSKNNTLSIETDSTQPANSHTSKTSNFPFYSPSPLPSLFKTSPA-ISS 61

Query: 1923 VTSTXXXXXXXXXXXXXPAKHIKALLARRHGSVKPNEATIPEGSECEIVGLDKNFGYSKS 1744
            V+ST             PAKHI+ALLARRHGSVKPNEA+IPEGSE +I  LDKNFG+SK 
Sbjct: 62   VSSTPLRIFKRPFPPPSPAKHIRALLARRHGSVKPNEASIPEGSESDIA-LDKNFGFSKQ 120

Query: 1743 FTSHYEIGEEVGRGHFGYTCSAKGKKGSLRGQDVAVKVIPKSKMTTAIAIEDVRREVKIL 1564
            F SHYE+GEEVGRGHFGYTCSAK KKGSL+GQDVAVKVIPKSKMTTAIAIEDVRREVKIL
Sbjct: 121  FVSHYELGEEVGRGHFGYTCSAKAKKGSLKGQDVAVKVIPKSKMTTAIAIEDVRREVKIL 180

Query: 1563 RALTGHKNLVQFYEAYEDEENVYVVMELCNGGELLDRILSRGGKYAEEDAKVVMVQILNV 1384
            RALTGH NLVQFY+AYED++NVYVVMELC GGELLDRILSRGGKY+EEDAK VMVQIL+V
Sbjct: 181  RALTGHNNLVQFYDAYEDDDNVYVVMELCKGGELLDRILSRGGKYSEEDAKTVMVQILSV 240

Query: 1383 VAYCHLQGVVHRDLKPENFLFVSKDEHSSLKAIDFGLSDYVKPDERLNDIVGSAYYVAPE 1204
            VAYCHLQGVVHRDLKPENFLF +K+E+S LKAIDFGLSDYVK DERLNDIVGSAYYVAPE
Sbjct: 241  VAYCHLQGVVHRDLKPENFLFTTKEENSPLKAIDFGLSDYVKLDERLNDIVGSAYYVAPE 300

Query: 1203 VLHRSYGTEADMWSIGVIAYILLCGSRPFWARTESGIFRAVLKADPSFEEDPWPNLSPDA 1024
            VLHRSYGTEADMWSIGVIAYILLCGSRPFWARTESGIFRAVLKADPSF+E PWP+LSP+A
Sbjct: 301  VLHRSYGTEADMWSIGVIAYILLCGSRPFWARTESGIFRAVLKADPSFDEAPWPSLSPEA 360

Query: 1023 IDFVKQLLNKDYRKRLTAAQALSHPWLAGHHDVKIPLDMIMYRLVKAYVCSSSLRKAALG 844
            IDFVK+LLNKDYRKRLTAAQALSHPWLA HHD+KIPLDMI+Y+LVKAY+ SSSLRK+ALG
Sbjct: 361  IDFVKRLLNKDYRKRLTAAQALSHPWLANHHDLKIPLDMIVYKLVKAYISSSSLRKSALG 420

Query: 843  ALARTLTIPQLAYLREQFALLGPSKSGFISLQNFKTAMVKNSTDAVKDSRVLEYVNVVSS 664
            ALA+TLT+ QLAYLREQF LLGPSK+GFIS+QNFKTA++K+STDA+KDSRVL+YVN++S+
Sbjct: 421  ALAKTLTVAQLAYLREQFTLLGPSKNGFISMQNFKTAVIKHSTDAMKDSRVLDYVNMIST 480

Query: 663  LQYRKFDFEEFCAAAVSVHQLEGIASWEQHARSAYELFEKDGNRPIMIEELASELGLSPS 484
            LQYRK DFEEF A A+SVHQLEG+  WEQHAR AYELFEKDGNRPIMIEELASELGLS S
Sbjct: 481  LQYRKLDFEEFSAVAISVHQLEGMDCWEQHARRAYELFEKDGNRPIMIEELASELGLSRS 540

Query: 483  VPVHVVLQDWIRHSDGKLSFLGFVRLLHGVSSRSFQKA 370
            VPVHVVLQDWIRHSDGKLSFLGFVRLLHGVSSR+FQKA
Sbjct: 541  VPVHVVLQDWIRHSDGKLSFLGFVRLLHGVSSRTFQKA 578


>emb|CBI32784.3| unnamed protein product [Vitis vinifera]
          Length = 593

 Score =  907 bits (2344), Expect = 0.0
 Identities = 463/576 (80%), Positives = 502/576 (87%)
 Frame = -1

Query: 2097 LCHGKPIETPDNLSGNSINPGETNELTLNSTGKTXXXXXXXXXXXXXXXKNSPANSSSVT 1918
            L  GKPIE P   S + I PG+    T   T KT               KNSPANSS V+
Sbjct: 21   LTAGKPIENPQTQSQDLIIPGDGELSTNTQTTKTPKFPFYSPSPLPSGFKNSPANSS-VS 79

Query: 1917 STXXXXXXXXXXXXXPAKHIKALLARRHGSVKPNEATIPEGSECEIVGLDKNFGYSKSFT 1738
            ST             PAKHI+ALLARRHGSVKPNEATIPEG+ECE VGLDKNFG+SK F 
Sbjct: 80   STPLRIFKRPFPPPSPAKHIRALLARRHGSVKPNEATIPEGNECE-VGLDKNFGFSKQFV 138

Query: 1737 SHYEIGEEVGRGHFGYTCSAKGKKGSLRGQDVAVKVIPKSKMTTAIAIEDVRREVKILRA 1558
            +HYE+GEEVGRGHFGYT SAK KKGSL+GQDVAVKVI KSKMTTAIAIEDVRREVKILRA
Sbjct: 139  AHYEMGEEVGRGHFGYTSSAKAKKGSLKGQDVAVKVIAKSKMTTAIAIEDVRREVKILRA 198

Query: 1557 LTGHKNLVQFYEAYEDEENVYVVMELCNGGELLDRILSRGGKYAEEDAKVVMVQILNVVA 1378
            LTGHKNLVQFYEAYED++NVY+VMELC GGELLDRIL+RGGKY+EEDAK VMVQILNV A
Sbjct: 199  LTGHKNLVQFYEAYEDDDNVYIVMELCRGGELLDRILARGGKYSEEDAKAVMVQILNVTA 258

Query: 1377 YCHLQGVVHRDLKPENFLFVSKDEHSSLKAIDFGLSDYVKPDERLNDIVGSAYYVAPEVL 1198
            +CHLQGVVHRDLKPENFLF+SK+E+S LKAIDFGLSDYVKPDERLNDIVGSAYYVAPEVL
Sbjct: 259  FCHLQGVVHRDLKPENFLFISKEENSPLKAIDFGLSDYVKPDERLNDIVGSAYYVAPEVL 318

Query: 1197 HRSYGTEADMWSIGVIAYILLCGSRPFWARTESGIFRAVLKADPSFEEDPWPNLSPDAID 1018
            HRSYGTEADMWS+GVIAYILLCGSRPFWARTESGIF+AVLKADPSF+E PWP+LS DAID
Sbjct: 319  HRSYGTEADMWSVGVIAYILLCGSRPFWARTESGIFKAVLKADPSFDEAPWPSLSSDAID 378

Query: 1017 FVKQLLNKDYRKRLTAAQALSHPWLAGHHDVKIPLDMIMYRLVKAYVCSSSLRKAALGAL 838
            FVK+LLNKDYRKRLTAAQALSHPWLA +HDVKIPLDMI+Y+LVKAY+ SSSLRK+AL AL
Sbjct: 379  FVKRLLNKDYRKRLTAAQALSHPWLANYHDVKIPLDMIVYKLVKAYIFSSSLRKSALAAL 438

Query: 837  ARTLTIPQLAYLREQFALLGPSKSGFISLQNFKTAMVKNSTDAVKDSRVLEYVNVVSSLQ 658
            A+TL+I QLAY REQF LLGP+KSGF+SLQNFKTA+ KNSTDA+KDSRVLEYV++V SLQ
Sbjct: 439  AKTLSIAQLAYFREQFTLLGPNKSGFVSLQNFKTAVTKNSTDAIKDSRVLEYVSMVGSLQ 498

Query: 657  YRKFDFEEFCAAAVSVHQLEGIASWEQHARSAYELFEKDGNRPIMIEELASELGLSPSVP 478
            YRK DFEEFCAAA+SV QLEG+ SWEQHAR  YELFEKDGNRPIMIEELASELGLSPSVP
Sbjct: 499  YRKLDFEEFCAAAISVLQLEGMESWEQHARRGYELFEKDGNRPIMIEELASELGLSPSVP 558

Query: 477  VHVVLQDWIRHSDGKLSFLGFVRLLHGVSSRSFQKA 370
            VHVVLQDWIRHSDGKLSFLGFVRLLHG  SR+F KA
Sbjct: 559  VHVVLQDWIRHSDGKLSFLGFVRLLHG-PSRAFPKA 593


>ref|XP_004141122.1| PREDICTED: calcium/calmodulin-dependent serine/threonine-protein
            kinase 1-like [Cucumis sativus]
            gi|449489494|ref|XP_004158329.1| PREDICTED:
            calcium/calmodulin-dependent serine/threonine-protein
            kinase 1-like [Cucumis sativus]
          Length = 573

 Score =  900 bits (2325), Expect = 0.0
 Identities = 453/578 (78%), Positives = 504/578 (87%), Gaps = 2/578 (0%)
 Frame = -1

Query: 2097 LCHGKPIETPDNLSGNSINPGETNELTLNSTGKTXXXXXXXXXXXXXXXK--NSPANSSS 1924
            LCHGKPIE   N   NS NP   N +  + T KT                  +SPANSS 
Sbjct: 3    LCHGKPIE--QNPKPNSENP---NSVIQSETPKTPTNFPFYSPSPLPNLFKSSSPANSS- 56

Query: 1923 VTSTXXXXXXXXXXXXXPAKHIKALLARRHGSVKPNEATIPEGSECEIVGLDKNFGYSKS 1744
            +TST             PAKHI+ALLARRHGSVKPNEA+IPEGSEC+ V LDKNFGYSK 
Sbjct: 57   ITSTPLRLFKRPFPPPSPAKHIRALLARRHGSVKPNEASIPEGSECD-VALDKNFGYSKH 115

Query: 1743 FTSHYEIGEEVGRGHFGYTCSAKGKKGSLRGQDVAVKVIPKSKMTTAIAIEDVRREVKIL 1564
            F +HY++G+EVGRGHFGYTCSA+ KKGS +GQ VAVK+IPKSKMTTAIAIEDVRREVKIL
Sbjct: 116  FAAHYDLGDEVGRGHFGYTCSARAKKGSFKGQQVAVKIIPKSKMTTAIAIEDVRREVKIL 175

Query: 1563 RALTGHKNLVQFYEAYEDEENVYVVMELCNGGELLDRILSRGGKYAEEDAKVVMVQILNV 1384
            RALTGHKNLVQFY++YEDEEN+YVVMELC GGELLDRILSRGGKY+EEDAKV+MVQIL+V
Sbjct: 176  RALTGHKNLVQFYDSYEDEENIYVVMELCEGGELLDRILSRGGKYSEEDAKVIMVQILSV 235

Query: 1383 VAYCHLQGVVHRDLKPENFLFVSKDEHSSLKAIDFGLSDYVKPDERLNDIVGSAYYVAPE 1204
            VAYCHLQGVVHRDLKPENFLF SKDE S+LKAIDFGLSDYVKPDERLNDIVGSAYYVAPE
Sbjct: 236  VAYCHLQGVVHRDLKPENFLFTSKDETSTLKAIDFGLSDYVKPDERLNDIVGSAYYVAPE 295

Query: 1203 VLHRSYGTEADMWSIGVIAYILLCGSRPFWARTESGIFRAVLKADPSFEEDPWPNLSPDA 1024
            VLHRSYGTEADMWSIGVIAYILLCGSRPFWARTESGIFRAVLKADP+FEE PWP+LS DA
Sbjct: 296  VLHRSYGTEADMWSIGVIAYILLCGSRPFWARTESGIFRAVLKADPNFEEAPWPSLSIDA 355

Query: 1023 IDFVKQLLNKDYRKRLTAAQALSHPWLAGHHDVKIPLDMIMYRLVKAYVCSSSLRKAALG 844
            IDFVK+LLNKDYRKRLTAAQAL HPWLA H D+KIPLD I ++LV++Y+CSSSLRK+ALG
Sbjct: 356  IDFVKRLLNKDYRKRLTAAQALCHPWLADHQDIKIPLDTITFKLVRSYICSSSLRKSALG 415

Query: 843  ALARTLTIPQLAYLREQFALLGPSKSGFISLQNFKTAMVKNSTDAVKDSRVLEYVNVVSS 664
            ALA+TL+  QL YL++QF LLGP+K+G IS+QNFKTA++KNST+A+KDSRVL+Y NVVSS
Sbjct: 416  ALAKTLSAVQLGYLQKQFTLLGPNKNGLISMQNFKTALIKNSTEAIKDSRVLDYANVVSS 475

Query: 663  LQYRKFDFEEFCAAAVSVHQLEGIASWEQHARSAYELFEKDGNRPIMIEELASELGLSPS 484
            +QYRK DFEEFCAAA+S++QLEG+ SWEQHAR AY+ F+KDGNRPIMIEELASELGLSPS
Sbjct: 476  IQYRKLDFEEFCAAAISIYQLEGMESWEQHARHAYDHFDKDGNRPIMIEELASELGLSPS 535

Query: 483  VPVHVVLQDWIRHSDGKLSFLGFVRLLHGVSSRSFQKA 370
            VPVHVVLQDWIRHSDGKLSFLGFVRLLHGVSSR+FQKA
Sbjct: 536  VPVHVVLQDWIRHSDGKLSFLGFVRLLHGVSSRAFQKA 573


>ref|XP_003518984.1| PREDICTED: calcium/calmodulin-dependent serine/threonine-protein
            kinase 1-like [Glycine max]
          Length = 583

 Score =  892 bits (2305), Expect = 0.0
 Identities = 449/583 (77%), Positives = 506/583 (86%), Gaps = 7/583 (1%)
 Frame = -1

Query: 2097 LCHGKPIETPDNLSGNSINPGE------TNELTLNSTGKTXXXXXXXXXXXXXXXKNSPA 1936
            +CHGKPIET  +    + NP E      T      ++ K                 +S +
Sbjct: 3    ICHGKPIETQQSQRETN-NPSEFPSDHPTPTKPPKASSKFPFYSPSPLPNWFKNSPSSNS 61

Query: 1935 NSSSVTSTXXXXXXXXXXXXXPAKHIKALLARRHGSVKPNEATIPEGSECEIVGLDKNFG 1756
            N SSV+ST             PAKHI+ALLARRHGSVKPNEA IPEGSECE VGLDK+FG
Sbjct: 62   NPSSVSSTPLRIFKRPFPPPSPAKHIRALLARRHGSVKPNEAAIPEGSECE-VGLDKSFG 120

Query: 1755 YSKSFTSHYEIGEEVGRGHFGYTCSAKGKKGSLRGQDVAVKVIPKSKMTTAIAIEDVRRE 1576
            ++K F++HYE+ +EVGRGHFGYTCSAKGKKG+ +G DVAVKVIPK+KMTTAIAIEDVRRE
Sbjct: 121  FAKQFSAHYELSDEVGRGHFGYTCSAKGKKGAFKGVDVAVKVIPKAKMTTAIAIEDVRRE 180

Query: 1575 VKILRALTGHKNLVQFYEAYEDEENVYVVMELCNGGELLDRILSRGGKYAEEDAKVVMVQ 1396
            VKILRALTGHKNLVQFYEAYED+ NVY+VMELC GGELLDRILSRGGKY+EEDA+VVM+Q
Sbjct: 181  VKILRALTGHKNLVQFYEAYEDDANVYIVMELCKGGELLDRILSRGGKYSEEDARVVMIQ 240

Query: 1395 ILNVVAYCHLQGVVHRDLKPENFLFVSKDEHSSLKAIDFGLSDYVKPDERLNDIVGSAYY 1216
            IL+VVA+CHLQGVVHRDLKPENFLF SKD++SSLKAIDFGLSDYVKPDERLNDIVGSAYY
Sbjct: 241  ILSVVAFCHLQGVVHRDLKPENFLFTSKDDNSSLKAIDFGLSDYVKPDERLNDIVGSAYY 300

Query: 1215 VAPEVLHRSYGTEADMWSIGVIAYILLCGSRPFWARTESGIFRAVLKADPSFEEDPWPNL 1036
            VAPEVLHRSYGTEADMWSIGVIAYILLCGSRPFWARTESGIFRAVLKADPSF+E PWP+L
Sbjct: 301  VAPEVLHRSYGTEADMWSIGVIAYILLCGSRPFWARTESGIFRAVLKADPSFDEAPWPSL 360

Query: 1035 SPDAIDFVKQLLNKDYRKRLTAAQALSHPWLAGHH-DVKIPLDMIMYRLVKAYVCSSSLR 859
            S DA DFVK+LLNKDYRKRLTAAQALSHPWL  HH D++IPLDMI+++LVKAY+CSSSLR
Sbjct: 361  SVDAKDFVKRLLNKDYRKRLTAAQALSHPWLVNHHDDMRIPLDMIIHKLVKAYICSSSLR 420

Query: 858  KAALGALARTLTIPQLAYLREQFALLGPSKSGFISLQNFKTAMVKNSTDAVKDSRVLEYV 679
            K+AL ALA+TLT+ QL YLR+Q+ LLGP+KSG IS+QNFKTA++++STDA KDSRVLEYV
Sbjct: 421  KSALRALAKTLTVAQLTYLRDQYTLLGPNKSGLISMQNFKTAVLRSSTDASKDSRVLEYV 480

Query: 678  NVVSSLQYRKFDFEEFCAAAVSVHQLEGIASWEQHARSAYELFEKDGNRPIMIEELASEL 499
            ++VSS+QYRK DFEEFCAAA+SVHQLEG+ +WEQHAR AYELFEK+GNRPIMIEELASEL
Sbjct: 481  SMVSSIQYRKLDFEEFCAAAISVHQLEGMETWEQHARRAYELFEKEGNRPIMIEELASEL 540

Query: 498  GLSPSVPVHVVLQDWIRHSDGKLSFLGFVRLLHGVSSRSFQKA 370
            GLSPSVPVHVVLQDWIRHSDGKLSFLGFVRLLHGVS+R+FQKA
Sbjct: 541  GLSPSVPVHVVLQDWIRHSDGKLSFLGFVRLLHGVSARAFQKA 583