BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rheum21_contig00005094
         (484 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002512034.1| conserved hypothetical protein [Ricinus comm...   135   4e-30
ref|XP_002514618.1| hypothetical protein RCOM_1467930 [Ricinus c...   135   6e-30
emb|CBI27324.3| unnamed protein product [Vitis vinifera]              134   1e-29
ref|XP_004165504.1| PREDICTED: uncharacterized protein LOC101229...   134   2e-29
ref|XP_006484248.1| PREDICTED: uncharacterized protein LOC102622...   133   3e-29
ref|XP_006437863.1| hypothetical protein CICLE_v10030834mg [Citr...   133   3e-29
gb|EMJ23184.1| hypothetical protein PRUPE_ppa002846mg [Prunus pe...   132   6e-29
ref|XP_002314922.2| hypothetical protein POPTR_0010s14890g [Popu...   131   8e-29
ref|XP_004297205.1| PREDICTED: uncharacterized protein LOC101300...   131   1e-28
gb|EOY01601.1| Chaperone DnaJ-domain superfamily protein, putati...   130   2e-28
ref|XP_004144161.1| PREDICTED: uncharacterized protein LOC101218...   130   2e-28
ref|XP_002312345.2| hypothetical protein POPTR_0008s10850g [Popu...   129   5e-28
gb|EXB44890.1| hypothetical protein L484_026472 [Morus notabilis]     128   7e-28
ref|XP_006348651.1| PREDICTED: stress response protein NST1-like...   126   3e-27
gb|ESW25468.1| hypothetical protein PHAVU_003G038600g [Phaseolus...   124   2e-26
ref|XP_003520133.2| PREDICTED: lisH domain-containing protein C1...   123   2e-26
ref|XP_002887221.1| heat shock protein binding protein [Arabidop...   123   2e-26
ref|XP_006573168.1| PREDICTED: dentin sialophosphoprotein-like i...   122   4e-26
ref|XP_006573167.1| PREDICTED: dentin sialophosphoprotein-like i...   122   4e-26
ref|XP_004239023.1| PREDICTED: uncharacterized protein LOC101260...   121   8e-26

>ref|XP_002512034.1| conserved hypothetical protein [Ricinus communis]
           gi|223549214|gb|EEF50703.1| conserved hypothetical
           protein [Ricinus communis]
          Length = 632

 Score =  135 bits (341), Expect = 4e-30
 Identities = 70/105 (66%), Positives = 81/105 (77%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           Q+KDEENLNMKE LR EVRK+L  +E +C DMASLLR LG+ VG    P   EV  AYK+
Sbjct: 532 QRKDEENLNMKENLRTEVRKELYQLENACIDMASLLRGLGIQVGGGFHPLTQEVHAAYKR 591

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKYLPAQ*HSTKC 317
           A+LKFHPDRASRTD+RQ VEAEEKFKLI+RMK+K+L     ST C
Sbjct: 592 ALLKFHPDRASRTDIRQQVEAEEKFKLISRMKQKFL-----STSC 631


>ref|XP_002514618.1| hypothetical protein RCOM_1467930 [Ricinus communis]
           gi|223546222|gb|EEF47724.1| hypothetical protein
           RCOM_1467930 [Ricinus communis]
          Length = 451

 Score =  135 bits (340), Expect = 6e-30
 Identities = 63/96 (65%), Positives = 83/96 (86%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKDEE LN+KE+LR EVR++L+ +E +C DMASLLR LG+ +G   +PS TEV+TAYK+
Sbjct: 355 QKKDEETLNLKEQLRIEVRRELDKLEANCTDMASLLRGLGIDIGGGFYPSSTEVRTAYKQ 414

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKYL 290
           A+L+FHPDRASR+D+RQ +EAEEKFKLI+R KEK++
Sbjct: 415 ALLRFHPDRASRSDIRQQIEAEEKFKLISRAKEKFM 450


>emb|CBI27324.3| unnamed protein product [Vitis vinifera]
          Length = 620

 Score =  134 bits (338), Expect = 1e-29
 Identities = 66/96 (68%), Positives = 79/96 (82%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKDEEN+NMKE++R EVRK+L+ +E +C DMASLLR L +HVG    PS  EV  AYK+
Sbjct: 522 QKKDEENMNMKEKIRLEVRKELDKLEMTCSDMASLLRGLEIHVGGGFCPSSNEVHAAYKR 581

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKYL 290
           A+LKFHPDRASRTD+   VEAEEKFKLI+RMKEK+L
Sbjct: 582 ALLKFHPDRASRTDIYHQVEAEEKFKLISRMKEKFL 617


>ref|XP_004165504.1| PREDICTED: uncharacterized protein LOC101229023 [Cucumis sativus]
          Length = 393

 Score =  134 bits (336), Expect = 2e-29
 Identities = 67/98 (68%), Positives = 84/98 (85%), Gaps = 2/98 (2%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKDEEN+NMKE+LRA+VRK+L+++E +C DMASLLR LG+ VG+ L PS  EVQ AYK+
Sbjct: 295 QKKDEENINMKEQLRADVRKELSMLEMNCTDMASLLRGLGIQVGSGLQPSSQEVQAAYKR 354

Query: 183 AVLKFHPDRAS--RTDMRQLVEAEEKFKLITRMKEKYL 290
           A+LKFHPDR S   TD+RQLVEAEEKFKLI+ MK+K++
Sbjct: 355 ALLKFHPDRVSSTSTDIRQLVEAEEKFKLISHMKKKFM 392


>ref|XP_006484248.1| PREDICTED: uncharacterized protein LOC102622387 isoform X1 [Citrus
           sinensis] gi|568861520|ref|XP_006484249.1| PREDICTED:
           uncharacterized protein LOC102622387 isoform X2 [Citrus
           sinensis]
          Length = 715

 Score =  133 bits (334), Expect = 3e-29
 Identities = 64/96 (66%), Positives = 81/96 (84%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKDEEN+N+KE++R EVRK+L  +E +C DMASLLR LG+HVG +  P   EV  AYK+
Sbjct: 615 QKKDEENMNLKEKIRIEVRKELCKLETTCIDMASLLRALGIHVGGSFRPLSQEVHAAYKR 674

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKYL 290
           A+L+FHPDRAS+TD+RQ VEAEEKFKLI+RM+EK+L
Sbjct: 675 ALLRFHPDRASKTDVRQQVEAEEKFKLISRMREKFL 710


>ref|XP_006437863.1| hypothetical protein CICLE_v10030834mg [Citrus clementina]
           gi|567890687|ref|XP_006437864.1| hypothetical protein
           CICLE_v10030834mg [Citrus clementina]
           gi|557540059|gb|ESR51103.1| hypothetical protein
           CICLE_v10030834mg [Citrus clementina]
           gi|557540060|gb|ESR51104.1| hypothetical protein
           CICLE_v10030834mg [Citrus clementina]
          Length = 715

 Score =  133 bits (334), Expect = 3e-29
 Identities = 64/96 (66%), Positives = 81/96 (84%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKDEEN+N+KE++R EVRK+L  +E +C DMASLLR LG+HVG +  P   EV  AYK+
Sbjct: 615 QKKDEENMNLKEKIRIEVRKELCKLETTCIDMASLLRALGIHVGGSFRPLSQEVHAAYKR 674

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKYL 290
           A+L+FHPDRAS+TD+RQ VEAEEKFKLI+RM+EK+L
Sbjct: 675 ALLRFHPDRASKTDVRQQVEAEEKFKLISRMREKFL 710


>gb|EMJ23184.1| hypothetical protein PRUPE_ppa002846mg [Prunus persica]
          Length = 628

 Score =  132 bits (331), Expect = 6e-29
 Identities = 67/96 (69%), Positives = 79/96 (82%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKDEENLN+K++LRAE+RK+L  +E +C DMASLLR LG+ VG    P   EV  AYK+
Sbjct: 528 QKKDEENLNLKDQLRAEIRKELKRLELTCIDMASLLRGLGIQVGAGFCPLPHEVHAAYKR 587

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKYL 290
           A+LKFHPDRASRTD+RQ VEAEEKFKLI+RMKEK L
Sbjct: 588 ALLKFHPDRASRTDVRQQVEAEEKFKLISRMKEKLL 623


>ref|XP_002314922.2| hypothetical protein POPTR_0010s14890g [Populus trichocarpa]
           gi|550329836|gb|EEF01093.2| hypothetical protein
           POPTR_0010s14890g [Populus trichocarpa]
          Length = 724

 Score =  131 bits (330), Expect = 8e-29
 Identities = 67/101 (66%), Positives = 79/101 (78%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKDEENLNMKER R EVRK+L  +E +C +MASLLR LG+HV   L P   +V  AYK+
Sbjct: 624 QKKDEENLNMKERFRVEVRKELYRLEVTCFNMASLLRGLGIHVEGGLKPLPNQVHAAYKR 683

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKYLPAQ*H 305
           A+LK HPDRAS+TD+RQ VEAEEKFKLI+RMKEK+L    H
Sbjct: 684 ALLKLHPDRASKTDIRQQVEAEEKFKLISRMKEKFLSTSYH 724


>ref|XP_004297205.1| PREDICTED: uncharacterized protein LOC101300999 [Fragaria vesca
           subsp. vesca]
          Length = 659

 Score =  131 bits (329), Expect = 1e-28
 Identities = 67/97 (69%), Positives = 84/97 (86%), Gaps = 1/97 (1%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNN-LFPSLTEVQTAYK 179
           QKKDEENLNMK++ RAEVRK+LN++  +CK M+SLLR LG+ VG++ + PS  EV  AYK
Sbjct: 559 QKKDEENLNMKDKFRAEVRKELNILS-ACKSMSSLLRGLGIQVGSSGVQPSPQEVHAAYK 617

Query: 180 KAVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKYL 290
           +A+LKFHPDRASRTD+RQ+VEAEEKFKLI+RMKEK +
Sbjct: 618 RALLKFHPDRASRTDIRQMVEAEEKFKLISRMKEKLI 654


>gb|EOY01601.1| Chaperone DnaJ-domain superfamily protein, putative isoform 1
           [Theobroma cacao] gi|508709705|gb|EOY01602.1| Chaperone
           DnaJ-domain superfamily protein, putative isoform 1
           [Theobroma cacao]
          Length = 634

 Score =  130 bits (326), Expect = 2e-28
 Identities = 65/101 (64%), Positives = 81/101 (80%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKDEEN+N+KE+LR EVRK+L+ +E SC +MASLLR LG+ VG    P   EV  AYK+
Sbjct: 534 QKKDEENMNLKEQLRIEVRKELSQLELSCINMASLLRSLGIPVGGGFCPLSHEVHAAYKR 593

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKYLPAQ*H 305
           AVL+FHPDRAS+T++R+ VEAEEKFKLI+RMKEK+L    H
Sbjct: 594 AVLRFHPDRASKTNIREQVEAEEKFKLISRMKEKFLATSCH 634


>ref|XP_004144161.1| PREDICTED: uncharacterized protein LOC101218935, partial [Cucumis
           sativus]
          Length = 389

 Score =  130 bits (326), Expect = 2e-28
 Identities = 66/95 (69%), Positives = 81/95 (85%), Gaps = 2/95 (2%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKDEEN+NMKE+LRA+VRK+L+++E +C DMASLLR LG+ VG+ L PS  EVQ AYK+
Sbjct: 295 QKKDEENINMKEQLRADVRKELSMLEMNCTDMASLLRGLGIQVGSGLQPSSQEVQAAYKR 354

Query: 183 AVLKFHPDRAS--RTDMRQLVEAEEKFKLITRMKE 281
           A+LKFHPDR S   TD+RQLVEAEEKFKLI+ MK+
Sbjct: 355 ALLKFHPDRVSSTSTDIRQLVEAEEKFKLISHMKK 389


>ref|XP_002312345.2| hypothetical protein POPTR_0008s10850g [Populus trichocarpa]
           gi|566183350|ref|XP_006379715.1| hypothetical protein
           POPTR_0008s10850g [Populus trichocarpa]
           gi|550332810|gb|EEE89712.2| hypothetical protein
           POPTR_0008s10850g [Populus trichocarpa]
           gi|550332811|gb|ERP57512.1| hypothetical protein
           POPTR_0008s10850g [Populus trichocarpa]
          Length = 695

 Score =  129 bits (323), Expect = 5e-28
 Identities = 67/105 (63%), Positives = 81/105 (77%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKDEENLN+KER R EVRK+L  +E +C +MASLLR LG+HV     P   +V  AYK+
Sbjct: 595 QKKDEENLNIKERFRVEVRKELYRLEVTCINMASLLRGLGIHVEGGFQPLPNQVHAAYKR 654

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKYLPAQ*HSTKC 317
           A+LKFHPDRAS+TD+R+ VEAEEKFKLI+RMKEK+L     ST C
Sbjct: 655 ALLKFHPDRASKTDIRRQVEAEEKFKLISRMKEKFL-----STSC 694


>gb|EXB44890.1| hypothetical protein L484_026472 [Morus notabilis]
          Length = 691

 Score =  128 bits (322), Expect = 7e-28
 Identities = 64/105 (60%), Positives = 82/105 (78%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKDEEN+N+KE+LR E+R++++ +E +C DMASLLR LG+ V     P   +V  AYK+
Sbjct: 591 QKKDEENINLKEKLRGEIRQEISRLEITCIDMASLLRGLGIQVAGGFCPMSHDVHAAYKR 650

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKYLPAQ*HSTKC 317
           A+LKFHPDRAS+TD+RQ VEAEEKFKLI+RMKEK+L     ST C
Sbjct: 651 ALLKFHPDRASKTDIRQQVEAEEKFKLISRMKEKFL-----STSC 690


>ref|XP_006348651.1| PREDICTED: stress response protein NST1-like [Solanum tuberosum]
          Length = 544

 Score =  126 bits (317), Expect = 3e-27
 Identities = 63/98 (64%), Positives = 83/98 (84%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKD EN+N+KE++RAEVRK+L+ +E +C DMAS+L  LG+ VGN    +  EV+ AYKK
Sbjct: 449 QKKDVENMNLKEQIRAEVRKELSKLEMTCHDMASVLHGLGITVGNG---TSHEVRVAYKK 505

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKYLPA 296
           A+LKFHPDRAS++D++Q VEAEEKFKLI+RMK+KYLP+
Sbjct: 506 ALLKFHPDRASQSDLQQQVEAEEKFKLISRMKDKYLPS 543


>gb|ESW25468.1| hypothetical protein PHAVU_003G038600g [Phaseolus vulgaris]
          Length = 572

 Score =  124 bits (310), Expect = 2e-26
 Identities = 60/101 (59%), Positives = 77/101 (76%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKDEE +N+KE+LR ++RK LN +E  C DM SLLR LG+HVG +  P   EVQ AYK+
Sbjct: 472 QKKDEEFMNLKEQLRVDIRKGLNHLEMQCHDMTSLLRGLGIHVGGSFTPLPNEVQAAYKR 531

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKYLPAQ*H 305
           A+  FHPDRAS++D+R  VEAEEKFKLI+R+K+K+L    H
Sbjct: 532 ALFTFHPDRASKSDVRAQVEAEEKFKLISRLKDKFLLTSCH 572


>ref|XP_003520133.2| PREDICTED: lisH domain-containing protein C1711.05-like [Glycine
           max]
          Length = 556

 Score =  123 bits (309), Expect = 2e-26
 Identities = 60/95 (63%), Positives = 75/95 (78%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKDEE +N+KE+LR E++K LN +E  C DM SLLR LG+ VG +  P   EV  AYK+
Sbjct: 456 QKKDEEVMNLKEQLRVEIQKGLNQLEMRCHDMPSLLRGLGIQVGGSFIPLPNEVHAAYKR 515

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKY 287
           A+LKFHPDRAS+TD+R  VEAEEKFKLI+R+KEK+
Sbjct: 516 ALLKFHPDRASKTDVRAQVEAEEKFKLISRLKEKF 550


>ref|XP_002887221.1| heat shock protein binding protein [Arabidopsis lyrata subsp.
           lyrata] gi|297333062|gb|EFH63480.1| heat shock protein
           binding protein [Arabidopsis lyrata subsp. lyrata]
          Length = 593

 Score =  123 bits (309), Expect = 2e-26
 Identities = 61/95 (64%), Positives = 76/95 (80%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKDEE++NMKE++RAE+ K L L+E    +MASLLR LG+ VG  ++P   EV  AYK+
Sbjct: 494 QKKDEESMNMKEKVRAEITKSLKLLELKSFNMASLLRGLGIQVGGGIYPLPHEVHAAYKR 553

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKY 287
           AVLKFHPDRASR D++Q VEAEEKFKLI RMK+K+
Sbjct: 554 AVLKFHPDRASRGDIKQQVEAEEKFKLIARMKDKF 588


>ref|XP_006573168.1| PREDICTED: dentin sialophosphoprotein-like isoform X2 [Glycine max]
          Length = 562

 Score =  122 bits (307), Expect = 4e-26
 Identities = 61/101 (60%), Positives = 75/101 (74%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKDEE +N+KE+LR E++K LN +E  C DM SLLR LG+ VG +  P   EV  AYK+
Sbjct: 462 QKKDEEVMNLKEQLRVEIQKGLNQLEMRCHDMPSLLRGLGIQVGGSFIPLPNEVHAAYKR 521

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKYLPAQ*H 305
           A+LKFHPDRAS+TD+R  VEAEEKFKLI+R KEK+     H
Sbjct: 522 ALLKFHPDRASKTDVRAQVEAEEKFKLISRWKEKFAMTSCH 562


>ref|XP_006573167.1| PREDICTED: dentin sialophosphoprotein-like isoform X1 [Glycine max]
          Length = 572

 Score =  122 bits (307), Expect = 4e-26
 Identities = 61/101 (60%), Positives = 75/101 (74%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKDEE +N+KE+LR E++K LN +E  C DM SLLR LG+ VG +  P   EV  AYK+
Sbjct: 472 QKKDEEVMNLKEQLRVEIQKGLNQLEMRCHDMPSLLRGLGIQVGGSFIPLPNEVHAAYKR 531

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKYLPAQ*H 305
           A+LKFHPDRAS+TD+R  VEAEEKFKLI+R KEK+     H
Sbjct: 532 ALLKFHPDRASKTDVRAQVEAEEKFKLISRWKEKFAMTSCH 572


>ref|XP_004239023.1| PREDICTED: uncharacterized protein LOC101260227 [Solanum
           lycopersicum]
          Length = 545

 Score =  121 bits (304), Expect = 8e-26
 Identities = 62/97 (63%), Positives = 80/97 (82%)
 Frame = +3

Query: 3   QKKDEENLNMKERLRAEVRKKLNLVEGSCKDMASLLRKLGVHVGNNLFPSLTEVQTAYKK 182
           QKKD EN+N+KE++RAEVR +L+ +E +C DMAS+L  LG+ VG     +  EV+ AYKK
Sbjct: 450 QKKDVENMNLKEQIRAEVRMELSKLEITCHDMASVLSGLGITVGAG---TSHEVRAAYKK 506

Query: 183 AVLKFHPDRASRTDMRQLVEAEEKFKLITRMKEKYLP 293
           A+LKFHPDRASR+D++Q VEAEEKFKLI+RMK+KYLP
Sbjct: 507 ALLKFHPDRASRSDLQQQVEAEEKFKLISRMKDKYLP 543