BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rheum21_contig00003328
         (1395 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...   377   e-102
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2...   363   7e-98
gb|EXB39337.1| Isoamylase 2 [Morus notabilis]                         355   2e-95
gb|EMJ02949.1| hypothetical protein PRUPE_ppa001199mg [Prunus pe...   355   2e-95
ref|XP_006479763.1| PREDICTED: isoamylase 2, chloroplastic-like ...   353   1e-94
ref|XP_006444132.1| hypothetical protein CICLE_v10023436mg [Citr...   346   1e-92
ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic-like ...   343   1e-91
ref|XP_006355876.1| PREDICTED: isoamylase 2, chloroplastic [Sola...   341   5e-91
ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] gi|...   341   5e-91
ref|XP_006386822.1| hypothetical protein POPTR_0002s22530g [Popu...   338   2e-90
gb|EOX94834.1| Debranching enzyme 1 [Theobroma cacao]                 338   4e-90
ref|XP_004247144.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2...   330   7e-88
ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2...   320   1e-84
ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like ...   320   1e-84
ref|NP_171830.1| Isoamylase 2 [Arabidopsis thaliana] gi|42571321...   320   1e-84
gb|AAM98123.1| putative isoamylase [Arabidopsis thaliana]             319   2e-84
ref|XP_002339667.1| predicted protein [Populus trichocarpa]           317   6e-84
ref|XP_006303226.1| hypothetical protein CARUB_v10012076mg [Caps...   314   6e-83
ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like ...   308   5e-81
dbj|BAF52942.1| isoamylase-type starch-debranching enzyme 2 [Pha...   306   1e-80

>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score =  377 bits (968), Expect = e-102
 Identities = 211/424 (49%), Positives = 269/424 (63%), Gaps = 22/424 (5%)
 Frame = -1

Query: 1206 MATLLASFSIRSCCINKGVAAPWKPVPSVSHISSRVTKGEEDKSGALRKLVFGDTALYAR 1027
            M TL+ S  IR CC+N G     K V +  +I         +K    RK + G  A  A 
Sbjct: 1    MTTLIPSLVIRPCCVNCGARDSSKLVAATHYICGNKVAHGLEKMDLERKEILGVVAQNAT 60

Query: 1026 ESSYGKLKVKVNSL-----------LTTPFSQAAEVSSKYMFRTEGGGQLKVSIRKTDLK 880
             + +  +  KV++             +T  S+  E    Y+F TE GGQ+KV + K + K
Sbjct: 61   RNCFRNINWKVSATSHIAIEETENRYSTTESEELESRLNYLFLTEIGGQVKVIVGKKNKK 120

Query: 879  YSVYIEVSSLCLDSSNDELVLVWGVYTSDSSSFIPLDSDSITPSTRNNITGTPLTLISPG 700
            Y V IEVSSL L +S+++L+L WGV+ S+SS F+P+D  ++ P   +N T  P    S G
Sbjct: 121  YIVSIEVSSLQLYNSDNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTEIPFMERSSG 180

Query: 699  IYEVELDLQSYLAPFYLSFLLKTSLETLSDGSEIKSHRKANFCVPIGFDPGYAYPLGLSF 520
             + ++LD ++  APFYLSFLLK++L+T    S+I+SHRK NFC+P+GF  GY  PLGLSF
Sbjct: 181  SFALKLDFEANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFGRGYPAPLGLSF 240

Query: 519  SSDGLMNFAFFSRSANGVCLCLYSDSTSEKPALEIDLDPYVNRTGDIWHASISNSGDFVS 340
            SSDG  NFAFFSR+A GV LCLY D TS+KPALEIDLDPYVNRTGDIWHAS+ + G FVS
Sbjct: 241  SSDGSPNFAFFSRNAGGVVLCLY-DGTSDKPALEIDLDPYVNRTGDIWHASMESVGSFVS 299

Query: 339  YGYRIKGATTNGRG-----RKVILDPYAKVISKS---EHGL---PGLGELSNTPDFDWSG 193
            YGYR K A     G       V LDPYAK+I  S   +HGL   P LGEL   P F+W+ 
Sbjct: 300  YGYRCKEANLQDSGETLHTEHVHLDPYAKLIRNSFSDDHGLKPQPRLGELQKEPAFNWND 359

Query: 192  DVPPCIPMEELVVYRLNVMRFTGDKSSKLPPKYAGTFAGVTDKLEHFTKLGVNAILLEPV 13
            DV P IPME+LVVYRLNVM FT D+SS++    AGTF+G+ +KL HF  LGVNA+LLEP+
Sbjct: 360  DVHPYIPMEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKDLGVNAVLLEPI 419

Query: 12   SPFD 1
              FD
Sbjct: 420  FSFD 423


>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
            isoamylase, putative [Ricinus communis]
          Length = 872

 Score =  363 bits (933), Expect = 7e-98
 Identities = 204/425 (48%), Positives = 266/425 (62%), Gaps = 23/425 (5%)
 Frame = -1

Query: 1206 MATLLASFSIRSCCINKGVAAPWKPVPSVSHISSRVTKGEEDKSGALRKLVFGDTALYAR 1027
            MAT++ SF+IR C    G     K           VT  ++     L + +F     + +
Sbjct: 1    MATIIPSFAIRPCYCVCGATESSKLA---------VTGNKDFGRLVLDQRLFNQRLHFWK 51

Query: 1026 ESSYGKLKVKVNSLLTTP-------FSQAAEVS-----SKYMFRTEGGGQLKVSIRKTDL 883
            +S +  L  +V S    P       FS +A +      S Y+FRT+ GG +KV +RK + 
Sbjct: 52   QSPHWSLDSRVCSAARVPVQQTELRFSTSAPLDELNKVSTYLFRTQFGGHVKVLVRKKNA 111

Query: 882  KYSVYIEVSSLCLDSSNDELVLVWGVYTSDSSSFIPLDSDSITPSTRNNITGTPLTLISP 703
            KY+VYIEVSSL L +++  L+L+WG+Y SDSS F+PLDS +  P+ R     T L   S 
Sbjct: 112  KYAVYIEVSSLELGTTDYRLMLIWGIYRSDSSCFMPLDSQNFAPNARK--MDTALVQNSF 169

Query: 702  GIYEVELDLQSYLAPFYLSFLLKTSLETLSDGSEIKSHRKANFCVPIGFDPGYAYPLGLS 523
            G + +EL+ +    PFYLSFLLK+ L T + G EIK+H+ ANFCVPIGF+ G   PLGLS
Sbjct: 170  GTFALELEFEPKQTPFYLSFLLKSKLNTDASGLEIKNHKNANFCVPIGFNSGDPSPLGLS 229

Query: 522  FSSDGLMNFAFFSRSANGVCLCLYSDSTSEKPALEIDLDPYVNRTGDIWHASISNSGDFV 343
            FS+DG MNFAFFSR+  G+ LCLY DST++KPALE+DLDPYVNRTGD+WHAS+  +  F 
Sbjct: 230  FSTDGSMNFAFFSRNVEGLVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASLEGAWTFT 289

Query: 342  SYGYRIKGATTNGRGRK-----VILDPYAKVISKS--EHG----LPGLGELSNTPDFDWS 196
            SYGYR KGA   G   K     V+LDPYA+VI+ S  +HG       LG L   P F+W 
Sbjct: 290  SYGYRCKGAILQGNTSKVDMECVLLDPYARVIASSMTDHGSRLSAKYLGRLCEEPAFEWG 349

Query: 195  GDVPPCIPMEELVVYRLNVMRFTGDKSSKLPPKYAGTFAGVTDKLEHFTKLGVNAILLEP 16
             D+ P + ME+L+VYRLNV RFT  KS KL    AGTFAG+ +K++HF  LGVNA+LLEP
Sbjct: 350  SDIRPNLAMEKLIVYRLNVKRFTEHKSGKLYSDIAGTFAGLIEKMDHFRNLGVNAVLLEP 409

Query: 15   VSPFD 1
            + PFD
Sbjct: 410  IFPFD 414


>gb|EXB39337.1| Isoamylase 2 [Morus notabilis]
          Length = 825

 Score =  355 bits (912), Expect = 2e-95
 Identities = 183/330 (55%), Positives = 231/330 (70%), Gaps = 8/330 (2%)
 Frame = -1

Query: 966  QAAEVSSKYMFRTEGGGQLKVSIRKTDLKYSVYIEVSSLCLDSSNDELVLVWGVYTSDSS 787
            + AE  S Y+FRTE GG + V +RK  +  +VY+EVSSL L  S+  L L WG+Y SDSS
Sbjct: 45   EEAEKVSTYLFRTEVGGLVNVFVRKKKVNCTVYVEVSSLELPRSDGTLGLSWGIYRSDSS 104

Query: 786  SFIPLDSDSITPSTRNNITGTPLTLISPGIYEVELDLQSYLAPFYLSFLLKTSLETLSDG 607
            SF+P   ++ TP        TP T  S G Y VE + ++   P YLSFLLK+  +  S G
Sbjct: 105  SFLPSHFETSTP------VETPFTKNSFGRYTVEFEFEAKEIPCYLSFLLKSPRDNDSSG 158

Query: 606  SEIKSHRKANFCVPIGFDPGYAYPLGLSFSSDGLMNFAFFSRSANGVCLCLYSDSTSEKP 427
             +I+SHRK NFCVP+GF  G+  PLGLSFS DG +NF+ FSRSA  V LCLY D+T++ P
Sbjct: 159  LDIRSHRKTNFCVPLGFSSGHPTPLGLSFSRDGSLNFSIFSRSAESVVLCLYDDTTADNP 218

Query: 426  ALEIDLDPYVNRTGDIWHASISNSGDFVSYGYRIKGATTNGR---GRKVILDPYAKVISK 256
            ALE+DLDPYVNRTGDIWHAS  +S  FVSYGYR+KG+   G+     +++LDPYAK+I K
Sbjct: 219  ALELDLDPYVNRTGDIWHASFESSSTFVSYGYRLKGSRLKGKKDEDARIVLDPYAKIIGK 278

Query: 255  ---SEHGL--PGLGELSNTPDFDWSGDVPPCIPMEELVVYRLNVMRFTGDKSSKLPPKYA 91
               S+HG+    LG +S  P FDWSGD+ P +P+E+L VYRLNVM FT  KSS+L P  A
Sbjct: 279  STSSDHGIGPKYLGRISKEPGFDWSGDIRPNLPIEKLTVYRLNVMHFTEHKSSQLSPDIA 338

Query: 90   GTFAGVTDKLEHFTKLGVNAILLEPVSPFD 1
            GTF+G+ +KLEHFT LGVNA+LLEP+ PFD
Sbjct: 339  GTFSGLIEKLEHFTDLGVNAVLLEPIFPFD 368


>gb|EMJ02949.1| hypothetical protein PRUPE_ppa001199mg [Prunus persica]
          Length = 883

 Score =  355 bits (912), Expect = 2e-95
 Identities = 204/425 (48%), Positives = 257/425 (60%), Gaps = 23/425 (5%)
 Frame = -1

Query: 1206 MATLLASFSIRSCCINKGVAAPWKPVPSVSHISSRVTKGEEDKSGALRKLVFGDTALYAR 1027
            MATL  S +I++CC N G+    K   +              K  A RKLVF +      
Sbjct: 1    MATLPLSIAIQACCFNCGITESSKLTATNGFRHRDKAMHGFVKLDAERKLVFREVVQNIN 60

Query: 1026 ESS--------YGKLKVKVNSLLTTPFSQAAEVS-----SKYMFRTEGGGQLKVSIRKTD 886
            E+         Y K +V V  +    FS   E       S Y+FRTE G  + V +RK +
Sbjct: 61   ENLPRDHNLKVYAKSQVSVEPM-EQRFSTGTETEEIDKVSTYLFRTEIGDVVNVFVRKRN 119

Query: 885  LKYSVYIEVSSLCLDSSNDELVLVWGVYTSDSSSFIPLDSDSITPSTRNNITGTPLTLIS 706
             KY+V IEV S  L S++  LVL WG+Y +DSS F+PLD  S TP+       TPL   S
Sbjct: 120  AKYTVNIEVPSSHLSSNDRRLVLRWGMYRADSSCFVPLDFKSSTPNDTTTTLETPLIQTS 179

Query: 705  PGIYEVELDLQSYLAPFYLSFLLKTSLETLSDGSEIKSHRKANFCVPIGFDPGYAYPLGL 526
             G + +EL+ ++   PFY SF+L +  +      EI+SHRK NFCVP+GF  GY  PLGL
Sbjct: 180  SGRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGL 239

Query: 525  SFSSDGLMNFAFFSRSANGVCLCLYSDSTSEKPALEIDLDPYVNRTGDIWHASISNSGDF 346
            +FS+DG MNFA FSR+A  V LCLY ++T+EKPALE+DLDPYVNR+GDIWH S  ++  F
Sbjct: 240  TFSNDGSMNFAIFSRNAESVALCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGSAWSF 299

Query: 345  VSYGYRIKG-----ATTNGRGRKVILDPYAKVISKS---EH--GLPGLGELSNTPDFDWS 196
            VSYGY+ KG        N     V+LDPYAKVI+KS    H  GL  LG L   P FDW+
Sbjct: 300  VSYGYKFKGNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGLKYLGRLCEEPAFDWA 359

Query: 195  GDVPPCIPMEELVVYRLNVMRFTGDKSSKLPPKYAGTFAGVTDKLEHFTKLGVNAILLEP 16
            GDV P + ME+LVVYRLNV RFT  KSS+LP    G+F+G+T+KLEHF  LGVNA+LLEP
Sbjct: 360  GDVRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLGVNAVLLEP 419

Query: 15   VSPFD 1
            + PFD
Sbjct: 420  IFPFD 424


>ref|XP_006479763.1| PREDICTED: isoamylase 2, chloroplastic-like isoform X1 [Citrus
            sinensis] gi|568852193|ref|XP_006479764.1| PREDICTED:
            isoamylase 2, chloroplastic-like isoform X2 [Citrus
            sinensis] gi|568852195|ref|XP_006479765.1| PREDICTED:
            isoamylase 2, chloroplastic-like isoform X3 [Citrus
            sinensis]
          Length = 889

 Score =  353 bits (906), Expect = 1e-94
 Identities = 201/430 (46%), Positives = 264/430 (61%), Gaps = 28/430 (6%)
 Frame = -1

Query: 1206 MATLLASFSIRSCCINKGVAAPWKPVP------SVSH--ISSRVTKG------EEDKSGA 1069
            MATL  S + R CC N G      P        S SH    S+ T+       E+  S  
Sbjct: 1    MATLAPSLAFRPCCFNCGAVESSPPSKLCAASASASHYVFKSKCTRRPARLEVEKTLSAQ 60

Query: 1068 LRKLVFGDTALYARESSYGKLKVKVNSLLTTPFSQAAEVS--SKYMFRTEGGGQLKVSIR 895
            + +        +   +S  ++ V     + TP ++  E+   S Y+FRT+ GG + VS+ 
Sbjct: 61   VCQNAVTTRPDFRACASSSRVSVDETQPVFTPTTEVDELQKVSTYLFRTDLGGLVSVSVG 120

Query: 894  KTDLKYSVYIEVSSLCLDSSNDELVLVWGVYTSDSSSFIPLDSDSITPSTRNNITGTPLT 715
            + ++ Y+V I+VSSL       +LVL+WGVYTSDSSS++ L S   TP  +     TP T
Sbjct: 121  EKNVNYAVTIDVSSLQTTRGGHKLVLIWGVYTSDSSSYMHLGSQCFTPDAKTGSIETPFT 180

Query: 714  LISPGIYEVELDLQSYLAPFYLSFLLKTSLETLSDGSEIKSHRKANFCVPIGFDPGYAYP 535
              S   + +EL  ++   PFYLSFLLK+ L   S   EI++H  A FCVP+G + G   P
Sbjct: 181  PTSFDTFALELGFEAKQTPFYLSFLLKSPLRDGSGDFEIRNHLSAKFCVPVGLNAGVPSP 240

Query: 534  LGLSFSSDGLMNFAFFSRSANGVCLCLYSDSTSEKPALEIDLDPYVNRTGDIWHASISNS 355
            +GLSFS DG +NFA FSR+A GV LCLY D+T+++PALE+DLDPY+NR+GDIWHAS+ ++
Sbjct: 241  MGLSFSIDGSLNFAIFSRNAQGVVLCLYDDTTADRPALELDLDPYINRSGDIWHASMEST 300

Query: 354  GDFVSYGYRIKGATTNGRGRK-----VILDPYAKVISKS-----EHGLPG--LGELSNTP 211
             +FVSYGYR KG+ + G G K     V+LDPYAK+I  S     + GLP   LG L   P
Sbjct: 301  WNFVSYGYRFKGSFSQGDGYKSHLESVLLDPYAKIIVNSIPNHHDLGLPPKYLGRLCKEP 360

Query: 210  DFDWSGDVPPCIPMEELVVYRLNVMRFTGDKSSKLPPKYAGTFAGVTDKLEHFTKLGVNA 31
            DFDW GDV   +PME+LVVYRLNVMRF+  KSSKLPP  AGTF+GVT+K+ H   LGVNA
Sbjct: 361  DFDWGGDVHLNLPMEKLVVYRLNVMRFSEHKSSKLPPDIAGTFSGVTEKVHHLKDLGVNA 420

Query: 30   ILLEPVSPFD 1
            ILLEP+  FD
Sbjct: 421  ILLEPILSFD 430


>ref|XP_006444132.1| hypothetical protein CICLE_v10023436mg [Citrus clementina]
            gi|557546394|gb|ESR57372.1| hypothetical protein
            CICLE_v10023436mg [Citrus clementina]
          Length = 840

 Score =  346 bits (888), Expect = 1e-92
 Identities = 196/416 (47%), Positives = 251/416 (60%), Gaps = 14/416 (3%)
 Frame = -1

Query: 1206 MATLLASFSIRSCCINKGVAAPWKPVPSVSHISSRVTKGEEDKSGALRKLVFGDTALYAR 1027
            MATL  S + R CC N G      P                             + L A 
Sbjct: 1    MATLAPSLAFRPCCFNCGAVESSPP-----------------------------SKLCAA 31

Query: 1026 ESSYGKLKVKVNSLLTTPFSQAAEVS--SKYMFRTEGGGQLKVSIRKTDLKYSVYIEVSS 853
             +S     V       TP ++  E+   S Y+FRT+ GG + VS+ + ++ Y+V I+VSS
Sbjct: 32   SASASHYPV------FTPTTEVDELQKVSTYLFRTDLGGLVSVSVGEKNVNYAVTIDVSS 85

Query: 852  LCLDSSNDELVLVWGVYTSDSSSFIPLDSDSITPSTRNNITGTPLTLISPGIYEVELDLQ 673
            L       +LVL+WGVYTSDSSS++ L S   TP  +     TP T  S   + +EL  +
Sbjct: 86   LQTTRGGHKLVLIWGVYTSDSSSYMHLGSQCFTPDAKTGSIETPFTPTSFDTFALELGFE 145

Query: 672  SYLAPFYLSFLLKTSLETLSDGSEIKSHRKANFCVPIGFDPGYAYPLGLSFSSDGLMNFA 493
            +   PFYLSFLLK+ L   S   EI++H  A FCVP+G + G   P+GLSFS DG +NFA
Sbjct: 146  AKQTPFYLSFLLKSPLRDGSGDFEIRNHLSAKFCVPVGLNAGVPSPMGLSFSIDGSLNFA 205

Query: 492  FFSRSANGVCLCLYSDSTSEKPALEIDLDPYVNRTGDIWHASISNSGDFVSYGYRIKGAT 313
             FSR+A GV LCLY D+T+++PALE+DLDPY+NR+GDIWHAS+ ++ +FVSYGYR KG+ 
Sbjct: 206  IFSRNAQGVVLCLYDDTTADRPALELDLDPYINRSGDIWHASMESTWNFVSYGYRFKGSF 265

Query: 312  TNGRGRK-----VILDPYAKVISKS-----EHGLPG--LGELSNTPDFDWSGDVPPCIPM 169
            + G G K     V+LDPYAK+I  S     + GLP   LG L   PDFDW GDV   +PM
Sbjct: 266  SQGDGYKSHLESVLLDPYAKIIVNSIPNHHDLGLPPKYLGRLCKEPDFDWGGDVHLNLPM 325

Query: 168  EELVVYRLNVMRFTGDKSSKLPPKYAGTFAGVTDKLEHFTKLGVNAILLEPVSPFD 1
            E+LVVYRLNVMRF+  KSSKLPP  AGTF+GVT+K+ H   LGVNAILLEP+  FD
Sbjct: 326  EKLVVYRLNVMRFSEHKSSKLPPDIAGTFSGVTEKVHHLKDLGVNAILLEPILSFD 381


>ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic-like [Fragaria vesca subsp.
            vesca]
          Length = 868

 Score =  343 bits (879), Expect = 1e-91
 Identities = 196/417 (47%), Positives = 251/417 (60%), Gaps = 21/417 (5%)
 Frame = -1

Query: 1188 SFSIRSCCINKGVAAPWKPVPSVSHISSR--VTKGEEDKSGALRKLVFGDTALYARESSY 1015
            S + ++CC   G  +    + + +H   R   T G   K  A RKLVFG       +SS 
Sbjct: 4    SITPQACCWACGTTSESSKLTANTHYRDRKKTTLGLV-KLDAERKLVFGGFGQSFAQSSL 62

Query: 1014 GKLKVKVNSL-------LTTPFSQAAEVSSK---YMFRTEGGGQLKVSIRKTDLKYSVYI 865
                 +V +        +   F    E ++K   Y+FRTE G  + V +R   + YSVY+
Sbjct: 63   RGCHSRVQAASGVSIEPMEQNFPTGTEETNKVSTYLFRTENGDLINVFVRDNTVNYSVYV 122

Query: 864  EVSSLCLDSSNDELVLVWGVYTSDSSSFIPLDSDSITPSTRNNITGTPLTLISPGIYEVE 685
            E+SSL L S+ D LV+ WG+Y +DSSS  PLD              TP T  S G + +E
Sbjct: 123  ELSSLQLSSAGDRLVISWGMYRADSSSLKPLD-----------FIETPFTKTSSGSFTLE 171

Query: 684  LDLQSYLAPFYLSFLLKTSLETLSDGSEIKSHRKANFCVPIGFDPGYAYPLGLSFSSDGL 505
            L+ ++   PFYLSF +K+  +    G EI+SHRK NFCVP+GF  G   PLGLS+SSDG 
Sbjct: 172  LEFEAKQTPFYLSFTVKSLADANLSGLEIRSHRKTNFCVPVGFGRGCPTPLGLSYSSDGS 231

Query: 504  MNFAFFSRSANGVCLCLYSDSTSEKPALEIDLDPYVNRTGDIWHASISNSGDFVSYGYRI 325
            +NFA FSR+A  V LCLY D T+++PALE+DLDPYVNR+GDIWHAS  ++  F+SYGYR 
Sbjct: 232  INFAIFSRNAESVVLCLYDDPTAQEPALELDLDPYVNRSGDIWHASFESAWTFLSYGYRF 291

Query: 324  KGATTNGRGR----KVILDPYAKVISKS-----EHGLPGLGELSNTPDFDWSGDVPPCIP 172
            KG +           V+LDPYAKVI +S       GL  LG L   P FDW GDV P +P
Sbjct: 292  KGTSLRNTNSLDEGNVLLDPYAKVIDESIANNRGTGLKLLGRLCEEPVFDWDGDVRPLLP 351

Query: 171  MEELVVYRLNVMRFTGDKSSKLPPKYAGTFAGVTDKLEHFTKLGVNAILLEPVSPFD 1
            +E+LVVYRLN+ RFT  KSSKLP   AGTF+G+T KL+HF  LGVNAILLEP+ PFD
Sbjct: 352  IEKLVVYRLNLKRFTEHKSSKLPTNVAGTFSGLTQKLDHFQDLGVNAILLEPIFPFD 408


>ref|XP_006355876.1| PREDICTED: isoamylase 2, chloroplastic [Solanum tuberosum]
          Length = 878

 Score =  341 bits (874), Expect = 5e-91
 Identities = 191/415 (46%), Positives = 256/415 (61%), Gaps = 13/415 (3%)
 Frame = -1

Query: 1206 MATLLASFSIRSCCINKGVAAPWKPVPSVSHISSRVTKGEEDKSGALRKLVFGDTALYA- 1030
            MAT     ++ S  ++ G     K VPS S    ++         +LRKL   D      
Sbjct: 1    MATSPIQLAVHSRLLSYGSTESTKLVPSSSGNRGKIVC-------SLRKLELEDMNFSGI 53

Query: 1029 -----RESSYGKLKVKVNSLLTTPFSQAAEVSSKYMFRTEGGGQLKVSIRKTDLKYSVYI 865
                 +E+     + K  S        +A+    Y+FRT+ GGQ+KV + +T+ KY V +
Sbjct: 54   GRNNDQEAPRRAHRRKALSASRISLVPSAKRVPTYLFRTDIGGQVKVLVERTNGKYKVLV 113

Query: 864  EVSSLCLDSSNDELVLVWGVYTSDSSSFIPLDSDSITPSTRNNITGTPLTLISPGIYEVE 685
            EV  L L  ++ ELV+VWG++ SD+S F+PLD +      +++   TP      G   VE
Sbjct: 114  EVLPLELSYAHSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVE 173

Query: 684  LDLQSYLAPFYLSFLLKTSLETLSDGSEIKSHRKANFCVPIGFDPGYAYPLGLSFSSDGL 505
            LD ++ LAPFY+SF +K+ L +  + SEI+SHR  NF VP+G   G+  PLG+SF  DG 
Sbjct: 174  LDFEASLAPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGS 233

Query: 504  MNFAFFSRSANGVCLCLYSDSTSEKPALEIDLDPYVNRTGDIWHASISNSGDFVSYGYRI 325
            +NFA FSRSA  V LCLY D + EKP+LEIDLDPY+NR+GDIWHA++  S  F +YGYR 
Sbjct: 234  VNFALFSRSARSVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDCSLPFKTYGYRC 293

Query: 324  KGATTNGRGRKVILDPYAKVISK-------SEHGLPGLGELSNTPDFDWSGDVPPCIPME 166
            K ATT+G+G  V+LDPYAKVI +       SE     LGEL   P +DWSGDVPP +PME
Sbjct: 294  K-ATTSGKGELVLLDPYAKVIRRVIPRQGGSEIRPKYLGELCLEPGYDWSGDVPPSLPME 352

Query: 165  ELVVYRLNVMRFTGDKSSKLPPKYAGTFAGVTDKLEHFTKLGVNAILLEPVSPFD 1
            +L++YRLNV +FT DKSSKLP   AGTF+G+++K  HF  LGVNA+LLEP+ PFD
Sbjct: 353  KLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNAMLLEPIFPFD 407


>ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] gi|27728147|gb|AAN15318.1|
            isoamylase isoform 2 [Solanum tuberosum]
          Length = 878

 Score =  341 bits (874), Expect = 5e-91
 Identities = 191/415 (46%), Positives = 256/415 (61%), Gaps = 13/415 (3%)
 Frame = -1

Query: 1206 MATLLASFSIRSCCINKGVAAPWKPVPSVSHISSRVTKGEEDKSGALRKLVFGDTALYA- 1030
            MAT     ++ S  ++ G     K VPS S    ++         +LRKL   D      
Sbjct: 1    MATSPIQLAVHSRLLSYGSTESTKLVPSSSGNRGKIVC-------SLRKLELEDMNFSGI 53

Query: 1029 -----RESSYGKLKVKVNSLLTTPFSQAAEVSSKYMFRTEGGGQLKVSIRKTDLKYSVYI 865
                 +E+     + K  S        +A+    Y+FRT+ GGQ+KV + +T+ KY V +
Sbjct: 54   GRNNDQEAPRRAHRRKALSASRISLVPSAKRVPTYLFRTDIGGQVKVLVERTNGKYKVLV 113

Query: 864  EVSSLCLDSSNDELVLVWGVYTSDSSSFIPLDSDSITPSTRNNITGTPLTLISPGIYEVE 685
            EV  L L  ++ ELV+VWG++ SD+S F+PLD +      +++   TP      G   VE
Sbjct: 114  EVLPLELSYAHSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVE 173

Query: 684  LDLQSYLAPFYLSFLLKTSLETLSDGSEIKSHRKANFCVPIGFDPGYAYPLGLSFSSDGL 505
            LD ++ LAPFY+SF +K+ L +  + SEI+SHR  NF VP+G   G+  PLG+SF  DG 
Sbjct: 174  LDFEASLAPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGS 233

Query: 504  MNFAFFSRSANGVCLCLYSDSTSEKPALEIDLDPYVNRTGDIWHASISNSGDFVSYGYRI 325
            +NFA FSRSA  V LCLY D + EKP+LEIDLDPY+NR+GDIWHA++  S  F +YGYR 
Sbjct: 234  VNFALFSRSARSVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDCSLPFKTYGYRC 293

Query: 324  KGATTNGRGRKVILDPYAKVISK-------SEHGLPGLGELSNTPDFDWSGDVPPCIPME 166
            K ATT+G+G  V+LDPYAKVI +       SE     LGEL   P +DWSGDVPP +PME
Sbjct: 294  K-ATTSGKGELVLLDPYAKVIRRVIPRQGGSEIRPKYLGELCLEPGYDWSGDVPPSLPME 352

Query: 165  ELVVYRLNVMRFTGDKSSKLPPKYAGTFAGVTDKLEHFTKLGVNAILLEPVSPFD 1
            +L++YRLNV +FT DKSSKLP   AGTF+G+++K  HF  LGVNA+LLEP+ PFD
Sbjct: 353  KLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNAMLLEPIFPFD 407


>ref|XP_006386822.1| hypothetical protein POPTR_0002s22530g [Populus trichocarpa]
            gi|550345605|gb|ERP64619.1| hypothetical protein
            POPTR_0002s22530g [Populus trichocarpa]
          Length = 857

 Score =  338 bits (868), Expect = 2e-90
 Identities = 186/334 (55%), Positives = 228/334 (68%), Gaps = 12/334 (3%)
 Frame = -1

Query: 966  QAAEVSSKYMFRTEGGGQLKVSIRKTDLKYSVYIEVSSLCLDSS-NDELVLVWGVYTSDS 790
            Q  EVS+ Y FRTE GG +K+S+ K + KY+VY+EVSSL L +S N  L+L+WG+YTSDS
Sbjct: 79   QLKEVST-YRFRTEIGGHVKISVGKINGKYAVYVEVSSLELGASDNISLMLIWGIYTSDS 137

Query: 789  SSFIPLDSDSITPSTRNNITGTPLTLISPGIYEVELDLQSYLAPFYLSFLLKTSLETLSD 610
            S F+PLDS S   +       TPL   S   +  EL+ ++   PFYLSF LK +   +  
Sbjct: 138  SCFMPLDSSSHARTRE-----TPLLQNSCARFATELEFEAKQTPFYLSFFLKPTSSVV-- 190

Query: 609  GSEIKSHRKANFCVPIGFDPGYAYPLGLSFSSDGLMNFAFFSRSANGVCLCLYSDSTSEK 430
              EI++H K+NFCVPIGFD GY  PLGLSFS+DG MNFAFFSR+A G  LCLY DSTS K
Sbjct: 191  --EIRNHNKSNFCVPIGFDSGYPTPLGLSFSTDGSMNFAFFSRNAAGCVLCLYDDSTSGK 248

Query: 429  PALEIDLDPYVNRTGDIWHASISNSGDFVSYGYRIKGATTNGRGRK-----VILDPYAKV 265
            PALE+DLDPYVNR+GDIWHAS+  +  F+SYGYR KGA       K     V+LDPY+K+
Sbjct: 249  PALELDLDPYVNRSGDIWHASLEGAWTFLSYGYRCKGAALQSDADKFDAGRVLLDPYSKI 308

Query: 264  ISKS-EHGLPG-----LGELSNTPDFDWSGDVPPCIPMEELVVYRLNVMRFTGDKSSKLP 103
            I  S    + G     LG L   P FDWS DVPP + ME+LVVYR+NVMRFT D SS++ 
Sbjct: 309  IINSVTDNVSGLLPKYLGRLCKEPVFDWSDDVPPHLEMEKLVVYRVNVMRFTKDNSSQIS 368

Query: 102  PKYAGTFAGVTDKLEHFTKLGVNAILLEPVSPFD 1
               AGTFAG+ +KL HF  LGVNA+LLE + PFD
Sbjct: 369  SDAAGTFAGLIEKLSHFKNLGVNAVLLESIFPFD 402


>gb|EOX94834.1| Debranching enzyme 1 [Theobroma cacao]
          Length = 867

 Score =  338 bits (866), Expect = 4e-90
 Identities = 196/424 (46%), Positives = 248/424 (58%), Gaps = 22/424 (5%)
 Frame = -1

Query: 1206 MATLLASFSIRSCCINKGVAAPWKPVPSVSHISSRVTKGEEDKS----GALRKLVFGDTA 1039
            MATL  S +I   C+N G       +  +S ++  + K +  +S       RKL  G+ A
Sbjct: 1    MATLPPSLAIGPHCLNSGATE----LSKLSFVTRYLCKSKMGQSLQRIDVGRKLFSGEVA 56

Query: 1038 LYARESSYGKLKVKVNSLLTTPFSQAAEVS-----------SKYMFRTEGGGQLKVSIRK 892
                +S Y  L ++  +       Q  ++            S Y+FRTE GGQ+KV +RK
Sbjct: 57   QNVMQSRYWNLDLRCFAASRVSVEQTEQIFTSTQVDELKKLSTYLFRTEIGGQVKVFVRK 116

Query: 891  TDLKYSVYIEVSSLCLDSSNDELVLVWGVYTSDSSSFIPLDSDSITPSTRNNITGTPLTL 712
              + + V IEVSSL L     +LVL  G++ SD                +N I  TP   
Sbjct: 117  RSVNHVVDIEVSSLQLSGDESQLVLSGGIHRSDHD-------------IKNRIIETPFIA 163

Query: 711  ISPGIYEVELDLQSYLAPFYLSFLLKTSLETLSDGSEIKSHRKANFCVPIGFDPGYAYPL 532
             S     +EL+ ++  APFY SFLLK        GSEI++HRK NFCVP+GF+ GY  PL
Sbjct: 164  KSSSELALELEFEAKEAPFYFSFLLKAPSGANLSGSEIRTHRKTNFCVPVGFNQGYPAPL 223

Query: 531  GLSFSSDGLMNFAFFSRSANGVCLCLYSDSTSEKPALEIDLDPYVNRTGDIWHASISNSG 352
            GLSFS+DG MNFA +SR+A  + LCLY D+ SEKPALE+DLDP+VNRTGDIWHASI  S 
Sbjct: 224  GLSFSTDGSMNFAVYSRNAESLVLCLYDDTASEKPALELDLDPFVNRTGDIWHASIEGSW 283

Query: 351  DFVSYGYRIKGATTNGRGRKVILDPYAKVISKS-----EHG--LPGLGELSNTPDFDWSG 193
             FV YGYR KG T      +V+LDPYA++I  S     E G  L  LG L   P FDWS 
Sbjct: 284  TFVRYGYRCKGDTDAFNAERVLLDPYARIIGSSIPNHHESGLLLKHLGRLCKEPAFDWSS 343

Query: 192  DVPPCIPMEELVVYRLNVMRFTGDKSSKLPPKYAGTFAGVTDKLEHFTKLGVNAILLEPV 13
            DV P +PME+LVVYRLNV+ FT DKSSKLP    GTFAGVT+K++H   LGVNA+LLEP+
Sbjct: 344  DVCPNLPMEKLVVYRLNVLHFTEDKSSKLPADVVGTFAGVTEKVQHLKDLGVNAVLLEPI 403

Query: 12   SPFD 1
              FD
Sbjct: 404  FTFD 407


>ref|XP_004247144.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
            [Solanum lycopersicum]
          Length = 878

 Score =  330 bits (847), Expect = 7e-88
 Identities = 167/321 (52%), Positives = 219/321 (68%), Gaps = 7/321 (2%)
 Frame = -1

Query: 942  YMFRTEGGGQLKVSIRKTDLKYSVYIEVSSLCLDSSNDELVLVWGVYTSDSSSFIPLDSD 763
            Y+FRT+ G Q+KV + KT+ KY V +EV  L L  ++ ELV+VWG++ SD+S F+PLD +
Sbjct: 88   YLFRTDIGCQVKVFVEKTNGKYKVLVEVLPLELSDAHSELVMVWGLFRSDASCFMPLDLN 147

Query: 762  SITPSTRNNITGTPLTLISPGIYEVELDLQSYLAPFYLSFLLKTSLETLSDGSEIKSHRK 583
                  +++   TP      G   VELD +  L PFY+SF +K+ L +  + SEI+SHR 
Sbjct: 148  RRGADGKSSTVETPFVQGPSGKVTVELDFEVSLVPFYISFYMKSQLVSDMENSEIRSHRN 207

Query: 582  ANFCVPIGFDPGYAYPLGLSFSSDGLMNFAFFSRSANGVCLCLYSDSTSEKPALEIDLDP 403
             NF VP+G   G+  PLG+SF  DG +NFA +SRSA GV LCLY D + EKP+LEIDLDP
Sbjct: 208  TNFVVPVGLSSGHPAPLGISFQPDGYVNFALYSRSAKGVVLCLYDDISVEKPSLEIDLDP 267

Query: 402  YVNRTGDIWHASISNSGDFVSYGYRIKGATTNGRGRKVILDPYAKVISK-------SEHG 244
            Y+N++GDIWHA++  S  F +YGYR K A T+G+G  V+LDPYAKVI         SE  
Sbjct: 268  YINQSGDIWHAALDCSLPFKTYGYRFK-AATSGKGELVLLDPYAKVIRSVIPRQGGSEIR 326

Query: 243  LPGLGELSNTPDFDWSGDVPPCIPMEELVVYRLNVMRFTGDKSSKLPPKYAGTFAGVTDK 64
               LGEL   P +DWSGDVPP +PME+L++YR+NV  FT DKSSKLP   AGTF+G+++K
Sbjct: 327  PKYLGELCLEPGYDWSGDVPPSLPMEKLIIYRINVTHFTKDKSSKLPDNLAGTFSGISEK 386

Query: 63   LEHFTKLGVNAILLEPVSPFD 1
              H   LGVNA+LLEP+ PFD
Sbjct: 387  WHHLKDLGVNAMLLEPIFPFD 407


>ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
            [Cucumis sativus]
          Length = 885

 Score =  320 bits (819), Expect = 1e-84
 Identities = 191/428 (44%), Positives = 254/428 (59%), Gaps = 26/428 (6%)
 Frame = -1

Query: 1206 MATLLASFSIRSCCI-NKGVAAPWKPVPSVSHISSRVTKGEEDKSGALRKLVFGDTALYA 1030
            MAT L+S  I+  C+ N GV    K   S      + TK +  K    R L  G+  + A
Sbjct: 1    MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 1029 RESSYGKL-KVKVNSLLTTPFS-QAAEVSSK---------YMFRTEGGGQLKVSIRKTDL 883
             +SS+  L K    S ++   S Q   +  K         Y+FRTE G  + V + K   
Sbjct: 61   VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 882  KYSVYIEVSSLCLDSSNDELVLVWGVYTSDSSSFIPLDSDSITPSTRNNITGTPLTLISP 703
             ++V IEV S+ L S ++ L+L WGVY SDS+   P + +S  P      T TP    S 
Sbjct: 121  TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFESSPPDETTGATETPFVKTSE 179

Query: 702  GIYEVELDLQSYLAPFYLSFLLKTSLETLSDGSEIKSHRKANFCVPIGFDPGYAYPLGLS 523
            G + VEL+  +   PFYLSF+LK  +   S  SEI+SH+K +F VP+GF  GY  PLGLS
Sbjct: 180  GKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLS 239

Query: 522  FSSDGLMNFAFFSRSANGVCLCLYSDSTSEKPALEIDLDPYVNRTGDIWHASISNSGDFV 343
             S DG +NF+ FS SA  + LCLY+DSTSEKP LE+DLDPY+NR+G+IWHAS   +  FV
Sbjct: 240  ISGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFV 299

Query: 342  SYGYRIKGATTNGRG-----RKVILDPYAKVISKS-------EHGLPG--LGELSNTPDF 205
            SYGY+ KG+ ++         ++++DPYAK+++ S         GLP   LG++S  P F
Sbjct: 300  SYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTF 359

Query: 204  DWSGDVPPCIPMEELVVYRLNVMRFTGDKSSKLPPKYAGTFAGVTDKLEHFTKLGVNAIL 25
            DW G+V P +PME+L VYRLNV RFT DKSS+LP   AGTF+G+T KL HF  LGVNA+L
Sbjct: 360  DWDGEVHPNLPMEKLXVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVL 419

Query: 24   LEPVSPFD 1
            LEP+  FD
Sbjct: 420  LEPIFQFD 427


>ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like [Cucumis sativus]
          Length = 885

 Score =  320 bits (819), Expect = 1e-84
 Identities = 191/428 (44%), Positives = 254/428 (59%), Gaps = 26/428 (6%)
 Frame = -1

Query: 1206 MATLLASFSIRSCCI-NKGVAAPWKPVPSVSHISSRVTKGEEDKSGALRKLVFGDTALYA 1030
            MAT L+S  I+  C+ N GV    K   S      + TK +  K    R L  G+  + A
Sbjct: 1    MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 1029 RESSYGKL-KVKVNSLLTTPFS-QAAEVSSK---------YMFRTEGGGQLKVSIRKTDL 883
             +SS+  L K    S ++   S Q   +  K         Y+FRTE G  + V + K   
Sbjct: 61   VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 882  KYSVYIEVSSLCLDSSNDELVLVWGVYTSDSSSFIPLDSDSITPSTRNNITGTPLTLISP 703
             ++V IEV S+ L S ++ L+L WGVY SDS+   P + +S  P      T TP    S 
Sbjct: 121  TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFESSPPDETTGATETPFVKTSE 179

Query: 702  GIYEVELDLQSYLAPFYLSFLLKTSLETLSDGSEIKSHRKANFCVPIGFDPGYAYPLGLS 523
            G + VEL+  +   PFYLSF+LK  +   S  SEI+SH+K +F VP+GF  GY  PLGLS
Sbjct: 180  GKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLS 239

Query: 522  FSSDGLMNFAFFSRSANGVCLCLYSDSTSEKPALEIDLDPYVNRTGDIWHASISNSGDFV 343
             S DG +NF+ FS SA  + LCLY+DSTSEKP LE+DLDPY+NR+G+IWHAS   +  FV
Sbjct: 240  ISGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFV 299

Query: 342  SYGYRIKGATTNGRG-----RKVILDPYAKVISKS-------EHGLPG--LGELSNTPDF 205
            SYGY+ KG+ ++         ++++DPYAK+++ S         GLP   LG++S  P F
Sbjct: 300  SYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTF 359

Query: 204  DWSGDVPPCIPMEELVVYRLNVMRFTGDKSSKLPPKYAGTFAGVTDKLEHFTKLGVNAIL 25
            DW G+V P +PME+L VYRLNV RFT DKSS+LP   AGTF+G+T KL HF  LGVNA+L
Sbjct: 360  DWDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVL 419

Query: 24   LEPVSPFD 1
            LEP+  FD
Sbjct: 420  LEPIFQFD 427


>ref|NP_171830.1| Isoamylase 2 [Arabidopsis thaliana] gi|42571321|ref|NP_973751.1|
            Isoamylase 2 [Arabidopsis thaliana]
            gi|251764780|sp|Q8L735.2|ISOA2_ARATH RecName:
            Full=Isoamylase 2, chloroplastic; Short=AtISA2; AltName:
            Full=Protein DEBRANCHING ENZYME 1; Flags: Precursor
            gi|3850573|gb|AAC72113.1| Similar to gi|1652733 glycogen
            operon protein GlgX from Synechocystis sp. genome
            gb|D90908. ESTs gb|H36690, gb|AA712462, gb|AA651230 and
            gb|N95932 come from this gene [Arabidopsis thaliana]
            gi|332189436|gb|AEE27557.1| Isoamylase 2 [Arabidopsis
            thaliana] gi|332189437|gb|AEE27558.1| Isoamylase 2
            [Arabidopsis thaliana]
          Length = 882

 Score =  320 bits (819), Expect = 1e-84
 Identities = 182/418 (43%), Positives = 243/418 (58%), Gaps = 17/418 (4%)
 Frame = -1

Query: 1206 MATLLASFSIRSCCINKGVAAPWKPVPSVSHISSRVTKGEEDKSGAL--RKLVFGDTALY 1033
            MA    S  I SCC+N G+   WK  PS    + R  K +      +  RK   GD    
Sbjct: 1    MAAWSPSVGIGSCCLNNGITRTWK-FPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTS 59

Query: 1032 ARESSY------GKLKVKVNSLLTTPFSQAAEVS--SKYMFRTEGGGQLKVSIRKTDLKY 877
            A +S         K  +++   L++  ++A ++   + Y FRT+ G  +KV + K   KY
Sbjct: 60   ALQSYQFSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKY 119

Query: 876  SVYIEVSSLCLDSSN-DELVLVWGVYTSDSSSFIPLDSDSITPSTRNNITGTPLTLISPG 700
            S+ + VSSL L   +   LV+VWGVY SDSS F+PLD ++ +  ++ + T T     S  
Sbjct: 120  SILVYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLS 179

Query: 699  IYEVELDLQSYLAPFYLSFLLKTSLETLSDGSEIKSHRKANFCVPIGFDPGYAYPLGLSF 520
               + L+     +PFYLSF LK       DG E+ +HR  +FC+P+GF  G+  PLGLS 
Sbjct: 180  ELMLGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSS 239

Query: 519  S-SDGLMNFAFFSRSANGVCLCLYSDSTSEKPALEIDLDPYVNRTGDIWHASISNSGDFV 343
               D   NF+FFSRS+  V LCLY DST++KPALE+DLDPYVNRTGD+WHAS+ N+ DFV
Sbjct: 240  GPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFV 299

Query: 342  SYGYRIKGAT-----TNGRGRKVILDPYAKVISKSEHGLPGLGELSNTPDFDWSGDVPPC 178
             YGYR K         +  G  ++LDPYA V+ KS      LG LS +P FDW  DV P 
Sbjct: 300  RYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSV-SQKYLGSLSKSPSFDWGEDVSPN 358

Query: 177  IPMEELVVYRLNVMRFTGDKSSKLPPKYAGTFAGVTDKLEHFTKLGVNAILLEPVSPF 4
            IP+E+L+VYRLNV  FT  +SSKLP   AGTF+GV +K+ H   LG NA+LLEP+  F
Sbjct: 359  IPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSF 416


>gb|AAM98123.1| putative isoamylase [Arabidopsis thaliana]
          Length = 882

 Score =  319 bits (818), Expect = 2e-84
 Identities = 182/418 (43%), Positives = 243/418 (58%), Gaps = 17/418 (4%)
 Frame = -1

Query: 1206 MATLLASFSIRSCCINKGVAAPWKPVPSVSHISSRVTKGEEDKSGAL--RKLVFGDTALY 1033
            MA    S  I SCC+N G+   WK  PS    + R  K +      +  RK   GD    
Sbjct: 1    MAAWSPSVGIGSCCLNNGITRTWK-FPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTS 59

Query: 1032 ARESSY------GKLKVKVNSLLTTPFSQAAEVS--SKYMFRTEGGGQLKVSIRKTDLKY 877
            A +S         K  +++   L++  ++A ++   + Y FRT+ G  +KV + K   KY
Sbjct: 60   ALQSYQFSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKY 119

Query: 876  SVYIEVSSLCLDSSN-DELVLVWGVYTSDSSSFIPLDSDSITPSTRNNITGTPLTLISPG 700
            S+ + VSSL L   +   LV+VWGVY SDSS F+PLD ++ +  ++ + T T     S  
Sbjct: 120  SILVYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLS 179

Query: 699  IYEVELDLQSYLAPFYLSFLLKTSLETLSDGSEIKSHRKANFCVPIGFDPGYAYPLGLSF 520
               + L+     +PFYLSF LK       DG E+ +HR  +FC+P+GF  G+  PLGLS 
Sbjct: 180  ELMLGLEFDGKESPFYLSFHLKLVSGRDPDGREMLTHRDTDFCIPVGFTAGHPLPLGLSS 239

Query: 519  S-SDGLMNFAFFSRSANGVCLCLYSDSTSEKPALEIDLDPYVNRTGDIWHASISNSGDFV 343
               D   NF+FFSRS+  V LCLY DST++KPALE+DLDPYVNRTGD+WHAS+ N+ DFV
Sbjct: 240  GPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFV 299

Query: 342  SYGYRIKGAT-----TNGRGRKVILDPYAKVISKSEHGLPGLGELSNTPDFDWSGDVPPC 178
             YGYR K         +  G  ++LDPYA V+ KS      LG LS +P FDW  DV P 
Sbjct: 300  RYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSV-SQKYLGSLSKSPSFDWGEDVSPN 358

Query: 177  IPMEELVVYRLNVMRFTGDKSSKLPPKYAGTFAGVTDKLEHFTKLGVNAILLEPVSPF 4
            IP+E+L+VYRLNV  FT  +SSKLP   AGTF+GV +K+ H   LG NA+LLEP+  F
Sbjct: 359  IPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSF 416


>ref|XP_002339667.1| predicted protein [Populus trichocarpa]
          Length = 316

 Score =  317 bits (813), Expect = 6e-84
 Identities = 172/306 (56%), Positives = 209/306 (68%), Gaps = 12/306 (3%)
 Frame = -1

Query: 882 KYSVYIEVSSLCLDSS-NDELVLVWGVYTSDSSSFIPLDSDSITPSTRNNITGTPLTLIS 706
           KY+VY+EVSSL L +S N  L+L+WG+YTSDSS F+PLDS S   +       TPL   S
Sbjct: 4   KYAVYVEVSSLELGASDNISLMLIWGIYTSDSSCFMPLDSSSHARTRE-----TPLLQNS 58

Query: 705 PGIYEVELDLQSYLAPFYLSFLLKTSLETLSDGSEIKSHRKANFCVPIGFDPGYAYPLGL 526
              +  EL+ ++   PFYLSF LK +   +    EI++H K+NFCVPIGFD GY  PLGL
Sbjct: 59  CARFATELEFEAKQTPFYLSFFLKPTSSVV----EIRNHNKSNFCVPIGFDSGYPTPLGL 114

Query: 525 SFSSDGLMNFAFFSRSANGVCLCLYSDSTSEKPALEIDLDPYVNRTGDIWHASISNSGDF 346
           SFS+DG MNFAFFSR+A G  LCLY DSTS KPALE+DLDPYVNR+GDIWHAS+  +  F
Sbjct: 115 SFSTDGSMNFAFFSRNAAGCVLCLYDDSTSGKPALELDLDPYVNRSGDIWHASLEGAWTF 174

Query: 345 VSYGYRIKGATTNGRGRK-----VILDPYAKVISKS-EHGLPG-----LGELSNTPDFDW 199
           +SYGYR KGA       K     V+LDPY+K+I  S    + G     LG L   P FDW
Sbjct: 175 LSYGYRCKGAALQSDADKFDAGRVLLDPYSKIIINSVTDNVSGLLPKYLGRLCKEPVFDW 234

Query: 198 SGDVPPCIPMEELVVYRLNVMRFTGDKSSKLPPKYAGTFAGVTDKLEHFTKLGVNAILLE 19
           S DVPP + ME+LVVYR+NVMRFT D SS++    AGTFAG+ +KL HF  LGVNA+LLE
Sbjct: 235 SDDVPPNLEMEKLVVYRVNVMRFTKDNSSQISSDTAGTFAGLIEKLSHFKNLGVNAVLLE 294

Query: 18  PVSPFD 1
            + PFD
Sbjct: 295 SIFPFD 300


>ref|XP_006303226.1| hypothetical protein CARUB_v10012076mg [Capsella rubella]
            gi|482571937|gb|EOA36124.1| hypothetical protein
            CARUB_v10012076mg [Capsella rubella]
          Length = 882

 Score =  314 bits (804), Expect = 6e-83
 Identities = 183/417 (43%), Positives = 238/417 (57%), Gaps = 16/417 (3%)
 Frame = -1

Query: 1206 MATLLASFSIRSCCINKGVAAPWK-PVPSVSHISSRVTKGEEDKSGALRKLVFGDTALYA 1030
            MA    S  I S  ++ G+   WK P P      +R+  G E      RK   GD    A
Sbjct: 1    MAAWSPSVGIGSFSLHNGITRTWKFPSPRFFTGKNRIKLGSETLI-VTRKRFLGDLGTSA 59

Query: 1029 RESSY------GKLKVKVNSLLTTPFSQAAEVS--SKYMFRTEGGGQLKVSIRKTDLKYS 874
             +S         +  V++   ++T  +Q  +    + Y+FRT+ G  +KV + K   KYS
Sbjct: 60   LQSYQLSKICASRTSVELQDQVSTRRAQVYDPKKVTSYLFRTKSGALVKVKVEKKREKYS 119

Query: 873  VYIEVSSLCLDSSN-DELVLVWGVYTSDSSSFIPLDSDSITPSTRNNITGTPLTLISPGI 697
            + + VSSL L   +   LV+VWGVY SDSS F+PLD ++ +  +  + T TP    S   
Sbjct: 120  IMVHVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSHTHTTETPFVKSSSSE 179

Query: 696  YEVELDLQSYLAPFYLSFLLKTSLETLSDGSEIKSHRKANFCVPIGFDPGYAYPLGLSFS 517
              + L+     +PF+LSF LK       DG EI +HR  +FCVP+GF  G+  PLGLS  
Sbjct: 180  LMLGLEFDGKESPFFLSFHLKLVSGKDPDGQEILTHRDTDFCVPVGFTAGHPLPLGLSSG 239

Query: 516  -SDGLMNFAFFSRSANGVCLCLYSDSTSEKPALEIDLDPYVNRTGDIWHASISNSGDFVS 340
              D   NFAFFSR++  V LCLY DST++KPALE+DLDPYVNRTGD+WHAS+ N+ DFV 
Sbjct: 240  PDDDTWNFAFFSRNSTSVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVR 299

Query: 339  YGYRIKGATTNG-----RGRKVILDPYAKVISKSEHGLPGLGELSNTPDFDWSGDVPPCI 175
            YGYR K    +        + V LDPYA VI KS      LG L  TP FDW  DV P I
Sbjct: 300  YGYRCKEMAQSEEDEDVEAQPVALDPYATVIDKS-FSQKFLGSLLKTPSFDWGEDVSPNI 358

Query: 174  PMEELVVYRLNVMRFTGDKSSKLPPKYAGTFAGVTDKLEHFTKLGVNAILLEPVSPF 4
            P+E+L+VYRLNV  FT  KSSKLP    GTF+GV +K+ H   LG+NA+LLEP+  F
Sbjct: 359  PLEKLIVYRLNVKGFTQHKSSKLPSNVTGTFSGVAEKVNHLKTLGINAVLLEPIFSF 415


>ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
          Length = 865

 Score =  308 bits (788), Expect = 5e-81
 Identities = 165/328 (50%), Positives = 215/328 (65%), Gaps = 11/328 (3%)
 Frame = -1

Query: 951  SSKYMFRTE-GGGQLKVSIRKTDLKYSVYIEVSSLCLDSSNDE-LVLVWGVYTSDSSSFI 778
            S  Y+FRTE GGG +KV + + + +YSVYIE+SSL +     E L+L WGVY SDSS F+
Sbjct: 84   SLAYLFRTETGGGLVKVHVTRRNDRYSVYIEISSLDISGGVGEALLLCWGVYRSDSSCFV 143

Query: 777  PLDSDSITPSTRNNITGTPLTLISPGIYEVELDLQSYLAPFYLSFLLKTSLETLSDGSEI 598
             LD+  ++ +    +  +PL   S G + +EL+  +   P YLSF L +SL++   G EI
Sbjct: 144  DLDTIGLSENAAMGMNVSPLVQNSDGKFAIELEFDAKHVPLYLSFFLMSSLDS---GLEI 200

Query: 597  KSHRKANFCVPIGFDPGYAYPLGLSFSSDGLMNFAFFSRSANGVCLCLYSDSTSEKPALE 418
            +SHR+ NFC+P+G  PGY  PLG+S+S DG +NF+ FSR A  V LCLY ++  EKPALE
Sbjct: 201  RSHRRTNFCMPVGSLPGYPCPLGVSYSPDGSVNFSIFSRHAESVVLCLYDENGVEKPALE 260

Query: 417  IDLDPYVNRTGDIWHASISNSGDFVSYGYR-----IKGATTNGRGRKVILDPYAKVISKS 253
            +DLDPYVNRTGDIWH S  ++  FVSYGYR     +K    +G    V+LDPYAK++  S
Sbjct: 261  LDLDPYVNRTGDIWHVSFESAKGFVSYGYRCRRGVLKKNKDDGFAEHVVLDPYAKIVGNS 320

Query: 252  EHG----LPGLGELSNTPDFDWSGDVPPCIPMEELVVYRLNVMRFTGDKSSKLPPKYAGT 85
                   +  LG L   P FDW GD  P + ME+LVVYRLNV RFT  +SS+LP   AGT
Sbjct: 321  YPDGVGLVKNLGCLRKEPFFDWGGDRHPDLSMEKLVVYRLNVKRFTQHESSQLPSGLAGT 380

Query: 84   FAGVTDKLEHFTKLGVNAILLEPVSPFD 1
            F G+  K++HF  LGVNA+LLEPV  FD
Sbjct: 381  FTGLAKKVQHFKDLGVNAVLLEPVFTFD 408


>dbj|BAF52942.1| isoamylase-type starch-debranching enzyme 2 [Phaseolus vulgaris]
            gi|561035862|gb|ESW34392.1| hypothetical protein
            PHAVU_001G148700g [Phaseolus vulgaris]
          Length = 865

 Score =  306 bits (785), Expect = 1e-80
 Identities = 170/351 (48%), Positives = 216/351 (61%), Gaps = 14/351 (3%)
 Frame = -1

Query: 1011 KLKVKVNSLLTTPFSQAAEVSSK--YMFRTE-GGGQLKVSIRKTDLKYSVYIEVSSLCLD 841
            +L ++       PF++  ++     Y+FRTE GGG +KV + K   +Y VYIE+SSL ++
Sbjct: 61   RLSIEETEQQVEPFTRPEDLKGALAYLFRTETGGGLVKVYVTKKKDRYFVYIEISSLDVN 120

Query: 840  SSNDE--LVLVWGVYTSDSSSFIPLDSDSITPSTRNNITGTPLTLISPGIYEVELDLQSY 667
               D   LVL WGVY  DSS F+ +DS  ++ +    +  +PL   S   + VEL+  + 
Sbjct: 121  HCGDSETLVLCWGVYRGDSSCFVDMDSTGLSGNAAKRMNVSPLVQTSVCKFGVELEFDAK 180

Query: 666  LAPFYLSFLLKTSLETLSDGSEIKSHRKANFCVPIGFDPGYAYPLGLSFSSDGLMNFAFF 487
              P YLSF L +SL+    G EI SHR+ NFCVP+G  PGY  PLGLS+S DG +NFA F
Sbjct: 181  YVPLYLSFFLMSSLDA---GLEIISHRRTNFCVPVGLLPGYPGPLGLSYSPDGSVNFAIF 237

Query: 486  SRSANGVCLCLYSDSTSEKPALEIDLDPYVNRTGDIWHASISNSGDFVSYGYRIKGAT-- 313
            SR A  V LCLY +   EKPALE+DLDPYVNR+GDIWH S  +   FVSYGYR +G    
Sbjct: 238  SRHAESVVLCLYDEKGVEKPALEVDLDPYVNRSGDIWHVSFESVKSFVSYGYRCRGGVHK 297

Query: 312  -TNG--RGRKVILDPYAKVISKSEHG----LPGLGELSNTPDFDWSGDVPPCIPMEELVV 154
              NG      V+LDPYAK++  S       +  LG L   P FDW GD  P + MEELVV
Sbjct: 298  QNNGDSSAELVVLDPYAKIVGHSYPSGLELVQNLGWLGKEPAFDWGGDFLPDLSMEELVV 357

Query: 153  YRLNVMRFTGDKSSKLPPKYAGTFAGVTDKLEHFTKLGVNAILLEPVSPFD 1
            YRLNV RFT   SS+LP   AGTF G+ +K++HF  LGVNA+LLEPV   D
Sbjct: 358  YRLNVKRFTQHNSSQLPSGSAGTFTGLAEKVQHFKDLGVNAVLLEPVFTSD 408