BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia32_contig00020395
         (848 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_020551423.1| trimethylguanosine synthase isoform X3 [Sesa...   295   5e-97
ref|XP_011087846.1| trimethylguanosine synthase isoform X2 [Sesa...   295   5e-97
ref|XP_020551422.1| trimethylguanosine synthase isoform X1 [Sesa...   295   7e-97
gb|PIN11714.1| Methylase [Handroanthus impetiginosus]                 291   6e-96
ref|XP_022857091.1| trimethylguanosine synthase-like [Olea europ...   281   6e-92
ref|XP_012828296.1| PREDICTED: trimethylguanosine synthase [Eryt...   278   1e-90
ref|XP_022857390.1| trimethylguanosine synthase-like [Olea europ...   269   5e-87
emb|CDO97474.1| unnamed protein product [Coffea canephora]            259   4e-83
ref|XP_009775804.1| PREDICTED: trimethylguanosine synthase [Nico...   258   8e-83
gb|PHT41661.1| Trimethylguanosine synthase [Capsicum baccatum]        257   6e-82
gb|PHT98604.1| Trimethylguanosine synthase [Capsicum chinense]        254   4e-81
ref|XP_016539204.1| PREDICTED: trimethylguanosine synthase [Caps...   254   4e-81
ref|XP_006351802.1| PREDICTED: trimethylguanosine synthase [Sola...   254   5e-81
ref|XP_024184689.1| trimethylguanosine synthase-like isoform X1 ...   253   6e-81
ref|XP_019232622.1| PREDICTED: trimethylguanosine synthase-like ...   254   7e-81
ref|XP_016513586.1| PREDICTED: trimethylguanosine synthase-like ...   251   6e-80
ref|XP_009612017.1| PREDICTED: trimethylguanosine synthase [Nico...   251   6e-80
ref|XP_004230622.1| PREDICTED: trimethylguanosine synthase [Sola...   250   1e-79
ref|XP_010034054.1| PREDICTED: trimethylguanosine synthase [Euca...   249   2e-79
ref|XP_008228857.1| PREDICTED: trimethylguanosine synthase [Prun...   248   6e-79

>ref|XP_020551423.1| trimethylguanosine synthase isoform X3 [Sesamum indicum]
          Length = 258

 Score =  295 bits (754), Expect = 5e-97
 Identities = 146/195 (74%), Positives = 156/195 (80%)
 Frame = -1

Query: 761 MSPAKDPAVTLPXXXXXXXXXXXXXXXXXXXXXXXXXXXKEQENVTPLVKKYWLQRYDLF 582
           MSPAKD  V LP                           KE+ENVTPLVKKYWLQRYDLF
Sbjct: 1   MSPAKDSVVALPRTRKQRKRFKRKVKIKAKRRAQKLEKLKERENVTPLVKKYWLQRYDLF 60

Query: 581 SKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAFAGVGGNAIQFAKVCRHVIAV 402
           S YDVG+KLDEEGWFSVTPEEIA RQA RCAGAGVVIDAFAGVGGNAIQFAKVC  V+AV
Sbjct: 61  SMYDVGVKLDEEGWFSVTPEEIAARQAERCAGAGVVIDAFAGVGGNAIQFAKVCHRVVAV 120

Query: 401 EIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGDVVFLSPPWGGPSYKAKENFT 222
           ++DP+KV LA HNAKIYGV+D IDFIVGDFFQLAPFLKGDVVFLSPPWGGPSYKAKENFT
Sbjct: 121 DVDPNKVQLAFHNAKIYGVEDCIDFIVGDFFQLAPFLKGDVVFLSPPWGGPSYKAKENFT 180

Query: 221 LDLLKPKDGYAIFVV 177
           LDLLKPKDG+++F V
Sbjct: 181 LDLLKPKDGHSLFQV 195



 Score = 79.7 bits (195), Expect = 9e-14
 Identities = 36/42 (85%), Positives = 42/42 (100%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           +SLF+VAQAITPNIIMYLPRN+D+LQASELSWLSSPPLD+E+
Sbjct: 190 HSLFQVAQAITPNIIMYLPRNLDMLQASELSWLSSPPLDVEI 231


>ref|XP_011087846.1| trimethylguanosine synthase isoform X2 [Sesamum indicum]
          Length = 260

 Score =  295 bits (754), Expect = 5e-97
 Identities = 146/195 (74%), Positives = 156/195 (80%)
 Frame = -1

Query: 761 MSPAKDPAVTLPXXXXXXXXXXXXXXXXXXXXXXXXXXXKEQENVTPLVKKYWLQRYDLF 582
           MSPAKD  V LP                           KE+ENVTPLVKKYWLQRYDLF
Sbjct: 1   MSPAKDSVVALPRTRKQRKRFKRKVKIKAKRRAQKLEKLKERENVTPLVKKYWLQRYDLF 60

Query: 581 SKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAFAGVGGNAIQFAKVCRHVIAV 402
           S YDVG+KLDEEGWFSVTPEEIA RQA RCAGAGVVIDAFAGVGGNAIQFAKVC  V+AV
Sbjct: 61  SMYDVGVKLDEEGWFSVTPEEIAARQAERCAGAGVVIDAFAGVGGNAIQFAKVCHRVVAV 120

Query: 401 EIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGDVVFLSPPWGGPSYKAKENFT 222
           ++DP+KV LA HNAKIYGV+D IDFIVGDFFQLAPFLKGDVVFLSPPWGGPSYKAKENFT
Sbjct: 121 DVDPNKVQLAFHNAKIYGVEDCIDFIVGDFFQLAPFLKGDVVFLSPPWGGPSYKAKENFT 180

Query: 221 LDLLKPKDGYAIFVV 177
           LDLLKPKDG+++F V
Sbjct: 181 LDLLKPKDGHSLFQV 195



 Score = 79.7 bits (195), Expect = 9e-14
 Identities = 36/42 (85%), Positives = 42/42 (100%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           +SLF+VAQAITPNIIMYLPRN+D+LQASELSWLSSPPLD+E+
Sbjct: 190 HSLFQVAQAITPNIIMYLPRNLDMLQASELSWLSSPPLDVEI 231


>ref|XP_020551422.1| trimethylguanosine synthase isoform X1 [Sesamum indicum]
          Length = 271

 Score =  295 bits (754), Expect = 7e-97
 Identities = 146/195 (74%), Positives = 156/195 (80%)
 Frame = -1

Query: 761 MSPAKDPAVTLPXXXXXXXXXXXXXXXXXXXXXXXXXXXKEQENVTPLVKKYWLQRYDLF 582
           MSPAKD  V LP                           KE+ENVTPLVKKYWLQRYDLF
Sbjct: 1   MSPAKDSVVALPRTRKQRKRFKRKVKIKAKRRAQKLEKLKERENVTPLVKKYWLQRYDLF 60

Query: 581 SKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAFAGVGGNAIQFAKVCRHVIAV 402
           S YDVG+KLDEEGWFSVTPEEIA RQA RCAGAGVVIDAFAGVGGNAIQFAKVC  V+AV
Sbjct: 61  SMYDVGVKLDEEGWFSVTPEEIAARQAERCAGAGVVIDAFAGVGGNAIQFAKVCHRVVAV 120

Query: 401 EIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGDVVFLSPPWGGPSYKAKENFT 222
           ++DP+KV LA HNAKIYGV+D IDFIVGDFFQLAPFLKGDVVFLSPPWGGPSYKAKENFT
Sbjct: 121 DVDPNKVQLAFHNAKIYGVEDCIDFIVGDFFQLAPFLKGDVVFLSPPWGGPSYKAKENFT 180

Query: 221 LDLLKPKDGYAIFVV 177
           LDLLKPKDG+++F V
Sbjct: 181 LDLLKPKDGHSLFQV 195



 Score = 79.7 bits (195), Expect = 1e-13
 Identities = 36/42 (85%), Positives = 42/42 (100%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           +SLF+VAQAITPNIIMYLPRN+D+LQASELSWLSSPPLD+E+
Sbjct: 190 HSLFQVAQAITPNIIMYLPRNLDMLQASELSWLSSPPLDVEI 231


>gb|PIN11714.1| Methylase [Handroanthus impetiginosus]
          Length = 262

 Score =  291 bits (745), Expect(2) = 6e-96
 Identities = 147/195 (75%), Positives = 156/195 (80%)
 Frame = -1

Query: 761 MSPAKDPAVTLPXXXXXXXXXXXXXXXXXXXXXXXXXXXKEQENVTPLVKKYWLQRYDLF 582
           M PAKD A TL                            KE+ENVTPLV+KYWLQRYDLF
Sbjct: 1   MFPAKDSAATLSRTRKQRKRFKRRVKIKAKRRAQKSGILKEKENVTPLVEKYWLQRYDLF 60

Query: 581 SKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAFAGVGGNAIQFAKVCRHVIAV 402
           S YDVGIKLDEEGWFSVTPEEIA RQARRCA AGVVIDAFAGVGGNAIQFAKVC  V+AV
Sbjct: 61  SLYDVGIKLDEEGWFSVTPEEIAARQARRCASAGVVIDAFAGVGGNAIQFAKVCHRVVAV 120

Query: 401 EIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGDVVFLSPPWGGPSYKAKENFT 222
           +IDP+KVALALHNAKIYGV+D+IDFIVGDFFQLAPFLKGDVVFLSPPWGGPSYKA+ENFT
Sbjct: 121 DIDPNKVALALHNAKIYGVEDYIDFIVGDFFQLAPFLKGDVVFLSPPWGGPSYKARENFT 180

Query: 221 LDLLKPKDGYAIFVV 177
           LDLLKPKDG  +F V
Sbjct: 181 LDLLKPKDGRYLFQV 195



 Score = 89.4 bits (220), Expect(2) = 6e-96
 Identities = 45/57 (78%), Positives = 50/57 (87%), Gaps = 3/57 (5%)
 Frame = -2

Query: 181 LFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEVH---VPLVPIILHFLPI 20
           LF+VAQAITPNIIMYLPRNIDVLQASELSWLSSPPLD+EVH   +P V ++  FLPI
Sbjct: 192 LFQVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDVEVHDYLLPPVTLVHRFLPI 248


>ref|XP_022857091.1| trimethylguanosine synthase-like [Olea europaea var. sylvestris]
          Length = 253

 Score =  281 bits (720), Expect = 6e-92
 Identities = 132/155 (85%), Positives = 147/155 (94%)
 Frame = -1

Query: 641 EQENVTPLVKKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAF 462
           E+ENVTPLV+KYWLQRYDLFS+YD GIK+DEEGWFSVTPEEIA RQAR CAGAGVVIDAF
Sbjct: 41  EKENVTPLVRKYWLQRYDLFSRYDEGIKMDEEGWFSVTPEEIAARQARWCAGAGVVIDAF 100

Query: 461 AGVGGNAIQFAKVCRHVIAVEIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGD 282
           AGVGGNAIQFA++C HV+A++IDP KVALA  NAKIYGV+D+IDFIVGDFFQLAPFLKGD
Sbjct: 101 AGVGGNAIQFAQMCHHVVAIDIDPKKVALASDNAKIYGVEDYIDFIVGDFFQLAPFLKGD 160

Query: 281 VVFLSPPWGGPSYKAKENFTLDLLKPKDGYAIFVV 177
           VVFLSPPWGGPSYKAKE+FTLDLLKPKDG+++F V
Sbjct: 161 VVFLSPPWGGPSYKAKESFTLDLLKPKDGHSLFQV 195



 Score = 76.6 bits (187), Expect = 1e-12
 Identities = 34/42 (80%), Positives = 41/42 (97%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           +SLF+VAQAITPNIIMYLPRN+D+LQA+ LSWLSSPPLD+E+
Sbjct: 190 HSLFQVAQAITPNIIMYLPRNVDLLQATALSWLSSPPLDVEI 231


>ref|XP_012828296.1| PREDICTED: trimethylguanosine synthase [Erythranthe guttata]
 gb|EYU18503.1| hypothetical protein MIMGU_mgv1a024884mg [Erythranthe guttata]
          Length = 253

 Score =  278 bits (712), Expect = 1e-90
 Identities = 134/153 (87%), Positives = 143/153 (93%)
 Frame = -1

Query: 641 EQENVTPLVKKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAF 462
           E+ NVTPLV KYWLQRYDLFSKYDVGIKLDEEGWFSVTPE IA  QA RCAGAGVVIDAF
Sbjct: 41  EKANVTPLVTKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEVIAACQAGRCAGAGVVIDAF 100

Query: 461 AGVGGNAIQFAKVCRHVIAVEIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGD 282
           AGVGGNAIQFAKVC  VIAVEIDP KVALA+HNAKIYGV+D IDFIVGDFFQLAPFLKGD
Sbjct: 101 AGVGGNAIQFAKVCSRVIAVEIDPEKVALAIHNAKIYGVEDCIDFIVGDFFQLAPFLKGD 160

Query: 281 VVFLSPPWGGPSYKAKENFTLDLLKPKDGYAIF 183
           VVFLSPPWGGPSYKA+++FTLDLLKPKDG+++F
Sbjct: 161 VVFLSPPWGGPSYKARQDFTLDLLKPKDGHSLF 193



 Score = 78.6 bits (192), Expect = 2e-13
 Identities = 36/42 (85%), Positives = 40/42 (95%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           +SLF+ AQAITPN+IMYLPRN+DVLQ SELSWLSSPPLDIEV
Sbjct: 190 HSLFQAAQAITPNVIMYLPRNVDVLQVSELSWLSSPPLDIEV 231


>ref|XP_022857390.1| trimethylguanosine synthase-like [Olea europaea var. sylvestris]
          Length = 246

 Score =  269 bits (687), Expect = 5e-87
 Identities = 125/155 (80%), Positives = 143/155 (92%)
 Frame = -1

Query: 641 EQENVTPLVKKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAF 462
           E+ENVTPLVKKYW QRYDLFS+YD GI +DEE WFSVTPEEIA +QA  CAGAGVVIDAF
Sbjct: 34  EKENVTPLVKKYWFQRYDLFSRYDEGIDMDEESWFSVTPEEIAAQQASWCAGAGVVIDAF 93

Query: 461 AGVGGNAIQFAKVCRHVIAVEIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGD 282
           AGVGGNAIQFA++C+ VIA++IDP KVA+A +NAKIYGV+ HIDFIVGDFFQ+APFLKGD
Sbjct: 94  AGVGGNAIQFAQMCQRVIAIDIDPKKVAMAYNNAKIYGVEHHIDFIVGDFFQVAPFLKGD 153

Query: 281 VVFLSPPWGGPSYKAKENFTLDLLKPKDGYAIFVV 177
           VVFLSPPWGGPSYKAKE+FTLDLLKPKDG+++F +
Sbjct: 154 VVFLSPPWGGPSYKAKESFTLDLLKPKDGHSLFQI 188



 Score = 64.3 bits (155), Expect = 2e-08
 Identities = 28/42 (66%), Positives = 39/42 (92%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           +SLF++A+AITP IIMYLPRN+D++QA+ LS LSSPPLD+++
Sbjct: 183 HSLFQIARAITPKIIMYLPRNVDLVQATGLSCLSSPPLDVKI 224


>emb|CDO97474.1| unnamed protein product [Coffea canephora]
          Length = 258

 Score =  259 bits (662), Expect = 4e-83
 Identities = 120/151 (79%), Positives = 137/151 (90%)
 Frame = -1

Query: 629 VTPLVKKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAFAGVG 450
           V+PLV+KYWLQRYDLF +YD GIKLDEEGWFSVTPEEIAV  A+RC GAGVVID FAGVG
Sbjct: 33  VSPLVEKYWLQRYDLFWRYDEGIKLDEEGWFSVTPEEIAVGHAQRCTGAGVVIDCFAGVG 92

Query: 449 GNAIQFAKVCRHVIAVEIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGDVVFL 270
           GNAIQFA+VC HV+A++IDP KVALA++NAK+YGV+DHIDFIVGDFFQLAP LKGDV+FL
Sbjct: 93  GNAIQFARVCDHVVAIDIDPEKVALAMNNAKVYGVEDHIDFIVGDFFQLAPSLKGDVLFL 152

Query: 269 SPPWGGPSYKAKENFTLDLLKPKDGYAIFVV 177
           SPPWGGPSYK  + FTLD LKPKDG+++F V
Sbjct: 153 SPPWGGPSYKMTKKFTLDSLKPKDGHSLFQV 183



 Score = 72.8 bits (177), Expect = 3e-11
 Identities = 38/66 (57%), Positives = 47/66 (71%)
 Frame = -2

Query: 259 GGAHHIRQKKILLSTY*SQKMGMRYSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSP 80
           GG  +   KK  L    S K    +SLF+VAQ ITP+IIMYLPRN+D+L+  +LSWLSSP
Sbjct: 157 GGPSYKMTKKFTLD---SLKPKDGHSLFQVAQKITPHIIMYLPRNVDLLEVEQLSWLSSP 213

Query: 79  PLDIEV 62
           PLDIE+
Sbjct: 214 PLDIEI 219


>ref|XP_009775804.1| PREDICTED: trimethylguanosine synthase [Nicotiana sylvestris]
 ref|XP_016515636.1| PREDICTED: trimethylguanosine synthase-like [Nicotiana tabacum]
 ref|XP_016515637.1| PREDICTED: trimethylguanosine synthase-like [Nicotiana tabacum]
          Length = 256

 Score =  258 bits (660), Expect = 8e-83
 Identities = 121/154 (78%), Positives = 138/154 (89%)
 Frame = -1

Query: 638 QENVTPLVKKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAFA 459
           +E V+PLVKKYW+QRYDLF +YD GIK+DEEGWFSVTPE+IAVR A RC G GVVIDAFA
Sbjct: 47  EEGVSPLVKKYWVQRYDLFLRYDEGIKMDEEGWFSVTPEQIAVRHAARCGG-GVVIDAFA 105

Query: 458 GVGGNAIQFAKVCRHVIAVEIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGDV 279
           GVGGNAIQFA +C HVIA++IDP KVALA  NAK+YGV++HIDFIVGDFFQLAP LKG+V
Sbjct: 106 GVGGNAIQFATMCHHVIAIDIDPRKVALAFENAKVYGVEEHIDFIVGDFFQLAPSLKGNV 165

Query: 278 VFLSPPWGGPSYKAKENFTLDLLKPKDGYAIFVV 177
           VFLSPPWGGP+YKA E FTLDLLKPK+GY++F V
Sbjct: 166 VFLSPPWGGPAYKANETFTLDLLKPKNGYSLFQV 199



 Score = 68.9 bits (167), Expect = 6e-10
 Identities = 29/42 (69%), Positives = 37/42 (88%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           YSLF+VAQ+ITPN+IMYLPRN+D+ Q   LSWLSSPP+ +E+
Sbjct: 194 YSLFQVAQSITPNVIMYLPRNVDLQQVEGLSWLSSPPMKVEI 235


>gb|PHT41661.1| Trimethylguanosine synthase [Capsicum baccatum]
          Length = 273

 Score =  257 bits (656), Expect = 6e-82
 Identities = 119/155 (76%), Positives = 138/155 (89%)
 Frame = -1

Query: 641 EQENVTPLVKKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAF 462
           E+E V+PLVKKYW QRYDLF +YD GIK+DEEGWFSVTPE+IA+R A RC G GVVID F
Sbjct: 57  EEEGVSPLVKKYWFQRYDLFWRYDQGIKMDEEGWFSVTPEQIAIRHALRCGG-GVVIDGF 115

Query: 461 AGVGGNAIQFAKVCRHVIAVEIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGD 282
           AGVGGNAIQFA +C HVIA++IDP K+ALA  NAKIYGV+DHI+FIVGDFFQLAP LKG+
Sbjct: 116 AGVGGNAIQFATMCHHVIAIDIDPRKIALAFENAKIYGVEDHIEFIVGDFFQLAPSLKGN 175

Query: 281 VVFLSPPWGGPSYKAKENFTLDLLKPKDGYAIFVV 177
           VVFLSPPWGGP Y++KE+FTLDLLKPK+GY++F V
Sbjct: 176 VVFLSPPWGGPEYRSKESFTLDLLKPKNGYSLFQV 210



 Score = 72.8 bits (177), Expect = 3e-11
 Identities = 31/42 (73%), Positives = 38/42 (90%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           YSLF+VAQ+I PN+IMYLPRN+D+LQ  ELSWLSSPPL +E+
Sbjct: 205 YSLFQVAQSIAPNVIMYLPRNVDLLQVEELSWLSSPPLKVEI 246


>gb|PHT98604.1| Trimethylguanosine synthase [Capsicum chinense]
          Length = 271

 Score =  254 bits (650), Expect = 4e-81
 Identities = 119/155 (76%), Positives = 137/155 (88%)
 Frame = -1

Query: 641 EQENVTPLVKKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAF 462
           E+E V+PLVKKYW QRYDLF +YD GIK+DEEGWFSVTPE+IAVR A RC G GVVID F
Sbjct: 55  EEEGVSPLVKKYWFQRYDLFWRYDQGIKMDEEGWFSVTPEQIAVRHALRCGG-GVVIDGF 113

Query: 461 AGVGGNAIQFAKVCRHVIAVEIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGD 282
           AGVGGNAIQFA +C HVIA++IDP K+ALA  NAKIYGV+ HI+FIVGDFFQLAP LKG+
Sbjct: 114 AGVGGNAIQFATMCHHVIAIDIDPRKIALAFENAKIYGVEGHIEFIVGDFFQLAPSLKGN 173

Query: 281 VVFLSPPWGGPSYKAKENFTLDLLKPKDGYAIFVV 177
           VVFLSPPWGGP Y++KE+FTLDLLKPK+GY++F V
Sbjct: 174 VVFLSPPWGGPEYRSKESFTLDLLKPKNGYSLFQV 208



 Score = 72.8 bits (177), Expect = 3e-11
 Identities = 31/42 (73%), Positives = 38/42 (90%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           YSLF+VAQ+I PN+IMYLPRN+D+LQ  ELSWLSSPPL +E+
Sbjct: 203 YSLFQVAQSIAPNVIMYLPRNVDLLQVEELSWLSSPPLKVEI 244


>ref|XP_016539204.1| PREDICTED: trimethylguanosine synthase [Capsicum annuum]
 gb|PHT64179.1| hypothetical protein T459_32052 [Capsicum annuum]
          Length = 272

 Score =  254 bits (650), Expect = 4e-81
 Identities = 119/155 (76%), Positives = 137/155 (88%)
 Frame = -1

Query: 641 EQENVTPLVKKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAF 462
           E+E V+PLVKKYW QRYDLF +YD GIK+DEEGWFSVTPE+IAVR A RC G GVVID F
Sbjct: 56  EEEGVSPLVKKYWFQRYDLFWRYDQGIKMDEEGWFSVTPEQIAVRHALRCGG-GVVIDGF 114

Query: 461 AGVGGNAIQFAKVCRHVIAVEIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGD 282
           AGVGGNAIQFA +C HVIA++IDP K+ALA  NAKIYGV+ HI+FIVGDFFQLAP LKG+
Sbjct: 115 AGVGGNAIQFATMCHHVIAIDIDPRKIALAFENAKIYGVEGHIEFIVGDFFQLAPSLKGN 174

Query: 281 VVFLSPPWGGPSYKAKENFTLDLLKPKDGYAIFVV 177
           VVFLSPPWGGP Y++KE+FTLDLLKPK+GY++F V
Sbjct: 175 VVFLSPPWGGPEYRSKESFTLDLLKPKNGYSLFQV 209



 Score = 72.8 bits (177), Expect = 3e-11
 Identities = 31/42 (73%), Positives = 38/42 (90%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           YSLF+VAQ+I PN+IMYLPRN+D+LQ  ELSWLSSPPL +E+
Sbjct: 204 YSLFQVAQSIAPNVIMYLPRNVDLLQVEELSWLSSPPLKVEI 245


>ref|XP_006351802.1| PREDICTED: trimethylguanosine synthase [Solanum tuberosum]
          Length = 256

 Score =  254 bits (648), Expect = 5e-81
 Identities = 118/155 (76%), Positives = 137/155 (88%)
 Frame = -1

Query: 641 EQENVTPLVKKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAF 462
           + E V+PLVKKYW+QRYDLF +YD GIK+DEEGWFSVTPE+IA+R A RC G GVVID F
Sbjct: 40  KDEGVSPLVKKYWVQRYDLFLRYDQGIKMDEEGWFSVTPEKIALRHASRCGG-GVVIDGF 98

Query: 461 AGVGGNAIQFAKVCRHVIAVEIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGD 282
           AGVGGNAIQFA +C HVIA++IDP K+ALA  NAKIYGV+DHI+FIVGDFFQLAP LKG+
Sbjct: 99  AGVGGNAIQFATMCHHVIAIDIDPRKIALAFENAKIYGVEDHIEFIVGDFFQLAPSLKGN 158

Query: 281 VVFLSPPWGGPSYKAKENFTLDLLKPKDGYAIFVV 177
           VVFLSPPWGGP+Y  KE+FTLDLLKPK+GY++F V
Sbjct: 159 VVFLSPPWGGPAYSTKESFTLDLLKPKNGYSLFQV 193



 Score = 71.6 bits (174), Expect = 7e-11
 Identities = 30/42 (71%), Positives = 38/42 (90%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           YSLF+VAQ++ PNIIMYLPRN+D+LQ  EL+WLSSPPL +E+
Sbjct: 188 YSLFQVAQSVAPNIIMYLPRNVDLLQVEELAWLSSPPLKVEI 229


>ref|XP_024184689.1| trimethylguanosine synthase-like isoform X1 [Rosa chinensis]
 gb|PRQ50336.1| putative noranthrone synthase [Rosa chinensis]
          Length = 251

 Score =  253 bits (647), Expect = 6e-81
 Identities = 115/152 (75%), Positives = 137/152 (90%)
 Frame = -1

Query: 638 QENVTPLVKKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAFA 459
           +E V+PLV+KYW QRYDLFS+YD GIKLDE+GW+SVTPE+IA+RQA+RC  A VVID FA
Sbjct: 35  EEGVSPLVEKYWFQRYDLFSRYDEGIKLDEQGWYSVTPEQIAIRQAKRCENATVVIDCFA 94

Query: 458 GVGGNAIQFAKVCRHVIAVEIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGDV 279
           GVGGNAIQFA +C HV+A+EIDP KV +A++NA+IYGV+D+IDFIVGDFFQLAP LKGD+
Sbjct: 95  GVGGNAIQFASMCYHVVAIEIDPLKVNMAINNARIYGVEDYIDFIVGDFFQLAPSLKGDI 154

Query: 278 VFLSPPWGGPSYKAKENFTLDLLKPKDGYAIF 183
           VFLSPPWGGPSY+  + FTLDLL+PKDGYAIF
Sbjct: 155 VFLSPPWGGPSYRRVKKFTLDLLEPKDGYAIF 186



 Score = 71.2 bits (173), Expect = 9e-11
 Identities = 37/66 (56%), Positives = 46/66 (69%)
 Frame = -2

Query: 259 GGAHHIRQKKILLSTY*SQKMGMRYSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSP 80
           GG  + R KK  L      K G  Y++F+ AQAITPNIIM+LPRN+D+LQ  EL WLSSP
Sbjct: 162 GGPSYRRVKKFTLDLL-EPKDG--YAIFQAAQAITPNIIMFLPRNVDLLQVEELCWLSSP 218

Query: 79  PLDIEV 62
           PL+ E+
Sbjct: 219 PLEFEI 224


>ref|XP_019232622.1| PREDICTED: trimethylguanosine synthase-like [Nicotiana attenuata]
          Length = 264

 Score =  254 bits (648), Expect = 7e-81
 Identities = 118/154 (76%), Positives = 137/154 (88%)
 Frame = -1

Query: 638 QENVTPLVKKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAFA 459
           ++ ++PLVKKYW+QRYDLFS+YD GIK+DEEGWFSVTPE+IAVR A RC G GVVIDAF 
Sbjct: 47  EQGLSPLVKKYWVQRYDLFSRYDEGIKMDEEGWFSVTPEQIAVRHAARCGG-GVVIDAFT 105

Query: 458 GVGGNAIQFAKVCRHVIAVEIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGDV 279
           GVGGNAIQFA +C HVIA++IDP KVALA  NAK+YGV++HIDFIVGDFFQLAP LKG+V
Sbjct: 106 GVGGNAIQFATMCHHVIAIDIDPRKVALAFENAKVYGVEEHIDFIVGDFFQLAPSLKGNV 165

Query: 278 VFLSPPWGGPSYKAKENFTLDLLKPKDGYAIFVV 177
           VFLSPPWGGP+Y A E FTLDLLKPK+GY++F V
Sbjct: 166 VFLSPPWGGPAYIANETFTLDLLKPKNGYSLFQV 199



 Score = 72.0 bits (175), Expect = 5e-11
 Identities = 31/42 (73%), Positives = 38/42 (90%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           YSLF+VAQ+ITPNIIMYLPRN+D+ Q  ELSWLSSPP+ +E+
Sbjct: 194 YSLFQVAQSITPNIIMYLPRNVDLQQVEELSWLSSPPMKVEI 235


>ref|XP_016513586.1| PREDICTED: trimethylguanosine synthase-like [Nicotiana tabacum]
 ref|XP_016513587.1| PREDICTED: trimethylguanosine synthase-like [Nicotiana tabacum]
 ref|XP_016513588.1| PREDICTED: trimethylguanosine synthase-like [Nicotiana tabacum]
          Length = 256

 Score =  251 bits (641), Expect = 6e-80
 Identities = 118/154 (76%), Positives = 135/154 (87%)
 Frame = -1

Query: 638 QENVTPLVKKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAFA 459
           +E + PLVKKYW+QRYDLF +YD GIK+DEEGWFSVTPE+IAVR A RC G GVVIDAFA
Sbjct: 47  EEGLIPLVKKYWVQRYDLFLRYDEGIKMDEEGWFSVTPEQIAVRHAARCGG-GVVIDAFA 105

Query: 458 GVGGNAIQFAKVCRHVIAVEIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGDV 279
           GVGGNAIQFA +C HVIA++IDP KVALA  NAK+YGV++ IDFIVGDFFQLAP LKG+V
Sbjct: 106 GVGGNAIQFATMCHHVIAIDIDPRKVALAFENAKVYGVEERIDFIVGDFFQLAPSLKGNV 165

Query: 278 VFLSPPWGGPSYKAKENFTLDLLKPKDGYAIFVV 177
           VFLSPPWGGP+Y A E FTLDLLKPK+GY++F V
Sbjct: 166 VFLSPPWGGPAYMANETFTLDLLKPKNGYSLFQV 199



 Score = 71.6 bits (174), Expect = 7e-11
 Identities = 30/42 (71%), Positives = 38/42 (90%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           YSLF+VAQ+ITPN+IMYLPRN+D+ Q  ELSWLSSPP+ +E+
Sbjct: 194 YSLFQVAQSITPNVIMYLPRNVDLQQVEELSWLSSPPMKVEI 235


>ref|XP_009612017.1| PREDICTED: trimethylguanosine synthase [Nicotiana tomentosiformis]
 ref|XP_018629389.1| PREDICTED: trimethylguanosine synthase [Nicotiana tomentosiformis]
 ref|XP_018629390.1| PREDICTED: trimethylguanosine synthase [Nicotiana tomentosiformis]
 ref|XP_018629391.1| PREDICTED: trimethylguanosine synthase [Nicotiana tomentosiformis]
          Length = 256

 Score =  251 bits (641), Expect = 6e-80
 Identities = 118/154 (76%), Positives = 135/154 (87%)
 Frame = -1

Query: 638 QENVTPLVKKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAFA 459
           +E + PLVKKYW+QRYDLF +YD GIK+DEEGWFSVTPE+IAVR A RC G GVVIDAFA
Sbjct: 47  EEGLIPLVKKYWVQRYDLFLRYDEGIKMDEEGWFSVTPEQIAVRHAARCGG-GVVIDAFA 105

Query: 458 GVGGNAIQFAKVCRHVIAVEIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGDV 279
           GVGGNAIQFA +C HVIA++IDP KVALA  NAK+YGV++ IDFIVGDFFQLAP LKG+V
Sbjct: 106 GVGGNAIQFATMCHHVIAIDIDPRKVALAFENAKVYGVEERIDFIVGDFFQLAPSLKGNV 165

Query: 278 VFLSPPWGGPSYKAKENFTLDLLKPKDGYAIFVV 177
           VFLSPPWGGP+Y A E FTLDLLKPK+GY++F V
Sbjct: 166 VFLSPPWGGPAYMANETFTLDLLKPKNGYSLFQV 199



 Score = 71.2 bits (173), Expect = 9e-11
 Identities = 30/42 (71%), Positives = 37/42 (88%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           YSLF+VAQ ITPN+IMYLPRN+D+ Q  ELSWLSSPP+ +E+
Sbjct: 194 YSLFQVAQGITPNVIMYLPRNVDLQQVEELSWLSSPPIKVEI 235


>ref|XP_004230622.1| PREDICTED: trimethylguanosine synthase [Solanum lycopersicum]
          Length = 242

 Score =  250 bits (638), Expect = 1e-79
 Identities = 117/155 (75%), Positives = 135/155 (87%)
 Frame = -1

Query: 641 EQENVTPLVKKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAF 462
           + E V+PLVKKYW+QRYDLF +YD GIK+DEEGWFSVTPE+IA+R A RC G GVVID F
Sbjct: 24  KDEGVSPLVKKYWVQRYDLFLRYDQGIKMDEEGWFSVTPEKIALRHASRCGG-GVVIDGF 82

Query: 461 AGVGGNAIQFAKVCRHVIAVEIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGD 282
            GVGGNAIQFA +C HVIA++IDP K+ALA  NAKIYGV+DHI+FIVGDFFQLA  LKG+
Sbjct: 83  TGVGGNAIQFATMCHHVIAIDIDPKKIALAFENAKIYGVEDHIEFIVGDFFQLASSLKGN 142

Query: 281 VVFLSPPWGGPSYKAKENFTLDLLKPKDGYAIFVV 177
           VVFLSPPWGGPSY  KE+FTLDLLKPK+GY++F V
Sbjct: 143 VVFLSPPWGGPSYSTKESFTLDLLKPKNGYSLFQV 177



 Score = 70.9 bits (172), Expect = 1e-10
 Identities = 30/42 (71%), Positives = 38/42 (90%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           YSLF+VAQ++ PNIIMYLPRN+D+LQ  EL+WLSSPPL +E+
Sbjct: 172 YSLFQVAQSVAPNIIMYLPRNVDLLQVEELAWLSSPPLKVEM 213


>ref|XP_010034054.1| PREDICTED: trimethylguanosine synthase [Eucalyptus grandis]
 gb|KCW53924.1| hypothetical protein EUGRSUZ_J03135 [Eucalyptus grandis]
          Length = 247

 Score =  249 bits (637), Expect = 2e-79
 Identities = 114/149 (76%), Positives = 132/149 (88%)
 Frame = -1

Query: 629 VTPLVKKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAFAGVG 450
           ++PLVKKYWLQRY LFS+YD G++LDEEGW+SVTPEEIA+R A RC G   VID FAGVG
Sbjct: 38  ISPLVKKYWLQRYHLFSRYDEGVELDEEGWYSVTPEEIAIRHAERCRGK-TVIDCFAGVG 96

Query: 449 GNAIQFAKVCRHVIAVEIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGDVVFL 270
           GNAIQFA +CRHVIA++IDP K+ALALHNAK+YGV+D IDF+VGDFFQLAP LKGDVV+L
Sbjct: 97  GNAIQFAILCRHVIAIDIDPQKIALALHNAKVYGVEDRIDFVVGDFFQLAPSLKGDVVYL 156

Query: 269 SPPWGGPSYKAKENFTLDLLKPKDGYAIF 183
           SPPWGGPSYK  + F LDLLKPKDGY++F
Sbjct: 157 SPPWGGPSYKMIQQFNLDLLKPKDGYSVF 185



 Score = 66.6 bits (161), Expect = 3e-09
 Identities = 28/42 (66%), Positives = 36/42 (85%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           YS+F+ AQ ITPNI+M+LPRN+D+ Q  ELSWLSSPPL +E+
Sbjct: 182 YSVFQAAQRITPNIVMFLPRNMDLSQVEELSWLSSPPLHVEI 223


>ref|XP_008228857.1| PREDICTED: trimethylguanosine synthase [Prunus mume]
          Length = 251

 Score =  248 bits (634), Expect = 6e-79
 Identities = 116/152 (76%), Positives = 134/152 (88%)
 Frame = -1

Query: 638 QENVTPLVKKYWLQRYDLFSKYDVGIKLDEEGWFSVTPEEIAVRQARRCAGAGVVIDAFA 459
           +E +TPLV+KYW QRYDLFS+YD GIK+DE+GW+SVTPEEIA+  A+RC  A  VID FA
Sbjct: 35  EEGLTPLVEKYWFQRYDLFSRYDEGIKMDEQGWYSVTPEEIAITHAKRCENA-TVIDCFA 93

Query: 458 GVGGNAIQFAKVCRHVIAVEIDPHKVALALHNAKIYGVQDHIDFIVGDFFQLAPFLKGDV 279
           GVGGNAIQFA +C HV+A+EIDP KV +A++NAKIYGV+D+IDFIVGDFFQLAP LKGDV
Sbjct: 94  GVGGNAIQFASMCYHVVAIEIDPLKVDMAINNAKIYGVEDYIDFIVGDFFQLAPSLKGDV 153

Query: 278 VFLSPPWGGPSYKAKENFTLDLLKPKDGYAIF 183
           VFLSPPWGGPSYK  + FTLDLLKPKDGYAIF
Sbjct: 154 VFLSPPWGGPSYKWMKKFTLDLLKPKDGYAIF 185



 Score = 69.7 bits (169), Expect = 3e-10
 Identities = 29/42 (69%), Positives = 37/42 (88%)
 Frame = -2

Query: 187 YSLFKVAQAITPNIIMYLPRNIDVLQASELSWLSSPPLDIEV 62
           Y++F+ AQAITPNIIM+LPRN+D+LQ  EL WLSSPPL +E+
Sbjct: 182 YAIFQAAQAITPNIIMFLPRNVDLLQVEELCWLSSPPLQVEI 223