BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia32_contig00008811
         (1464 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_020550647.1| E4 SUMO-protein ligase PIAL2-like [Sesamum i...   551   0.0  
ref|XP_011073310.1| E4 SUMO-protein ligase PIAL2 [Sesamum indicum]    487   e-160
gb|PIN01560.1| hypothetical protein CDL12_25935 [Handroanthus im...   456   e-148
ref|XP_022880892.1| E4 SUMO-protein ligase PIAL2-like [Olea euro...   421   e-135
ref|XP_012834292.1| PREDICTED: E3 SUMO-protein ligase SIZ1 [Eryt...   385   e-121
ref|XP_022867111.1| E4 SUMO-protein ligase PIAL1-like isoform X1...   338   e-107
ref|XP_019247404.1| PREDICTED: E4 SUMO-protein ligase PIAL1-like...   331   e-102
ref|XP_019247402.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   329   e-101
ref|XP_019247401.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   329   e-101
ref|XP_019247405.1| PREDICTED: E4 SUMO-protein ligase PIAL1-like...   328   e-100
ref|XP_016454176.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   328   e-99 
ref|XP_016454175.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   328   1e-99
ref|XP_016552263.1| PREDICTED: E4 SUMO-protein ligase PIAL2 [Cap...   327   3e-99
ref|XP_009612350.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   327   3e-99
gb|PHT39733.1| hypothetical protein CQW23_18587 [Capsicum baccatum]   327   3e-99
ref|XP_009612349.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like...   327   3e-99
ref|XP_022867113.1| E4 SUMO-protein ligase PIAL2-like isoform X3...   317   3e-99
ref|XP_010324636.1| PREDICTED: E4 SUMO-protein ligase PIAL1 isof...   322   5e-99
ref|XP_009783337.1| PREDICTED: uncharacterized protein LOC104231...   326   9e-99
ref|XP_006352265.1| PREDICTED: uncharacterized protein LOC102591...   323   9e-98

>ref|XP_020550647.1| E4 SUMO-protein ligase PIAL2-like [Sesamum indicum]
          Length = 897

 Score =  551 bits (1421), Expect = 0.0
 Identities = 317/569 (55%), Positives = 374/569 (65%), Gaps = 82/569 (14%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCPISFRRI+TPVKGH CKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCF DIRIDQK+V
Sbjct: 328  NCPISFRRIRTPVKGHSCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFADIRIDQKMV 387

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTGDGESPQPVDLLDLTQTND 362
            KVLKEVEANVSDI+ SSDGSW AV +SD AIQKPE K S T + +SP+PVD+LDLTQT+D
Sbjct: 388  KVLKEVEANVSDIVFSSDGSWQAVKNSDAAIQKPEKKTSITENNDSPEPVDVLDLTQTDD 447

Query: 363  GMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGIFLSS 542
             MD   T ETEDRKLS++  Q+QS+TQT++V P IANT+ VN  S  VE DFWSG++LS+
Sbjct: 448  AMDNTITCETEDRKLSQTACQSQSVTQTMAVNPLIANTNYVNTSSTDVEYDFWSGVYLST 507

Query: 543  LGQG----PSNEQIGGVFVPTSNYIASPPVLTDSFTT-NGQVEGFNGNNLFTASVPQREP 707
             G      PSNE +  V V TSN+IASPP LTDSF + NG+VE F GN+L  ASVPQ E 
Sbjct: 508  FGLASSNFPSNELVAAVSVSTSNFIASPPGLTDSFVSPNGEVEAFPGNSLMPASVPQSEA 567

Query: 708  SLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQALPAQTSTSILQQRSR---- 875
            SLP+T QLQQ QFGN   T EYGR  S+PR++TR P+AVQALP Q S+S+LQQ SR    
Sbjct: 568  SLPHTSQLQQCQFGNVTITNEYGRLQSIPRHITRLPSAVQALPVQASSSVLQQMSRDNVN 627

Query: 876  -------NTARRAN----------LH----QVSQMSSSPLHQYSGVQNPGQENRSFPTVR 992
                   + A +A+          LH    Q+SQMSSSPLH Y  +    Q+ R FP V 
Sbjct: 628  ALIHNGLSAASQASPASPMISNPVLHPSPLQLSQMSSSPLHHYPRI----QQTRPFPYVS 683

Query: 993  PPHQNVVLQATNQVPDAQRVLNERQLSSQQMANLMMPRAMGQ------------------ 1118
            PP QN+VLQA+ QVP+A RVLN+R  SSQQM N  MPR+M Q                  
Sbjct: 684  PPQQNIVLQASGQVPNAPRVLNQR--SSQQMTNFRMPRSMSQSLDTNISSIQSPASLLRT 741

Query: 1119 ----------------------PP--AMSQPSYSMNPNG----------RRMPSVVGDQW 1196
                                  PP  A++  + +++ +G          RRMPS V DQ 
Sbjct: 742  QSDVGVSQDRIVHTTELVSGQHPPRTALADRTRAVDTSGAALSYSTTSDRRMPS-VSDQR 800

Query: 1197 SNVGVASPPLMTTDAYDPAQQTWRPAGRMRGALSGQAYADAYNRFMSQHNQQAAQAARPI 1376
            SN G+AS P  +T AYDP +Q WRPA RMRGALSGQAY +A N+++ + NQQ  Q  RP 
Sbjct: 801  SNFGIASQPSTSTGAYDPGEQNWRPAVRMRGALSGQAYTNALNQYIIRANQQ-TQVVRPT 859

Query: 1377 SNVHLDNVNVPAESFMPPNYPSARPASLP 1463
            SNV     NVPA S     YPSARP S P
Sbjct: 860  SNVTSVPDNVPALS-----YPSARPGSRP 883


>ref|XP_011073310.1| E4 SUMO-protein ligase PIAL2 [Sesamum indicum]
          Length = 886

 Score =  487 bits (1253), Expect = e-160
 Identities = 286/562 (50%), Positives = 345/562 (61%), Gaps = 75/562 (13%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCPISFRRIKTPVKGH CKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIR+DQ++V
Sbjct: 326  NCPISFRRIKTPVKGHSCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRVDQQMV 385

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTGDGESPQPVDLLDLTQTND 362
            K+LKE   NVS I++SSDGSWNAVM+ DT +Q PED+  + G+ E  QP D+LDLTQT+D
Sbjct: 386  KILKEAGPNVSTIVVSSDGSWNAVMEEDT-VQNPEDETFNAGEDEFSQPRDVLDLTQTDD 444

Query: 363  GMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGIFLSS 542
             +  ++  E EDRK   S +  Q M QT ++ PHIANT++VNQ S HV +DF SGI+  +
Sbjct: 445  AIYAISACEPEDRK-HFSDADLQFMDQTEAINPHIANTNDVNQSSTHVANDFRSGIYTPT 503

Query: 543  LGQG-PSNEQIGGVFVPTSNYIASP---PVLTDSFTTNGQ-VEGFNGNNLFTASVPQREP 707
            +G G PSN      F P  +    P     +T  FT+  Q VE F GN L TAS  Q   
Sbjct: 504  IGLGLPSN------FRPNPDIAGCPVSISAITHPFTSPSQEVEAFRGNALVTASTSQTAT 557

Query: 708  SLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQALPAQTSTSILQQRSRN--- 878
            SLPNT QLQQ+QFGN + T EYGRFPS+PR+++RTP AVQALPAQ  TS+ QQ SRN   
Sbjct: 558  SLPNTSQLQQYQFGNPSITNEYGRFPSVPRHLSRTPIAVQALPAQPPTSV-QQSSRNSVN 616

Query: 879  ---------------------TARRANLHQVSQMSSSPLHQYSGVQNPGQENRSFPTVRP 995
                                 T  RAN H+VSQMSSSPL Q   V    Q+NR F + RP
Sbjct: 617  ALTQNGLSAASPVSPSVPNSSTPLRANPHEVSQMSSSPLVQRPSV----QQNRPFLSARP 672

Query: 996  PHQNVVLQATNQVPDAQRVLNERQLSSQQMANLMMPRAMGQPPAMSQ------------- 1136
            PHQN   Q  NQ+P+A RV NE   SSQQM    +P    + P ++Q             
Sbjct: 673  PHQNAGSQNLNQIPNAYRVSNEHHSSSQQMVKPRVPHPRSRSPGLNQSSMQSSGSLLHSQ 732

Query: 1137 ----------------------PSYSMNPNGRRMPSVVGDQWSNVGVASPPLMTTDAYDP 1250
                                  PS S  P+GR M S +G+Q  N+GV S P+  TD YDP
Sbjct: 733  QTHLIAAANRTVQMAIGEPGISPSSSRKPDGRSMLS-LGNQMGNIGVTSSPVTRTDTYDP 791

Query: 1251 AQQTWRPAGRMRGALSGQAYADAYNRFMSQHNQQAAQAARPISNV---------HLDNVN 1403
            ++  WRPAGRMRGALSGQAY DA N+F+ + +QQ AQAARP+ N+         HL  V 
Sbjct: 792  SEVNWRPAGRMRGALSGQAYTDALNQFIIRPSQQ-AQAARPVPNLTSLPTNVQSHLQAVV 850

Query: 1404 VPAESFMPP--NYPSARPASLP 1463
                  + P  N+PSA PAS P
Sbjct: 851  AGEAVQVTPAQNHPSAGPASSP 872


>gb|PIN01560.1| hypothetical protein CDL12_25935 [Handroanthus impetiginosus]
          Length = 891

 Score =  456 bits (1173), Expect = e-148
 Identities = 271/573 (47%), Positives = 330/573 (57%), Gaps = 86/573 (15%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCP+SFRR+KTPVKGH CKHIQCFDFDNYVD+NSRRPSWRCPHCNQHVCFTD+RIDQ ++
Sbjct: 325  NCPMSFRRVKTPVKGHSCKHIQCFDFDNYVDMNSRRPSWRCPHCNQHVCFTDVRIDQNML 384

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTGDGESPQPVDLLDLTQTND 362
            K+LKEV ANVS I +SSDGSW+AVM+S+  IQKPED   +    +S QP D+LDLTQ +D
Sbjct: 385  KILKEVGANVSVITVSSDGSWSAVMESEETIQKPEDNTLNPEQDDSHQPADVLDLTQNDD 444

Query: 363  GMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGIFLSS 542
             MD V   ETEDRK   ++   Q +TQT  V PH+ANT++V Q      DDFWSGI + +
Sbjct: 445  AMDAVAAYETEDRKQFPNLHGGQFVTQTTVVNPHMANTNDVTQ------DDFWSGIHMPT 498

Query: 543  LG----QGPSNEQIGGVFVPTSNYIASPPVLTDSFTT-NGQVEGFNGNNLFTASVPQREP 707
             G       SN QIG       ++ AS  VL DSFT+ +   E F+G+ L   SVPQ E 
Sbjct: 499  FGLVSPNVRSNAQIG-------SFSASTSVLADSFTSPSTNAEAFHGSALVATSVPQGET 551

Query: 708  SLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQALPAQTSTSILQQRSRNTA- 884
            SLPNT+Q QQ+Q GN   T EYGRFPS+PR++TRTP A+QALPA+TS S++QQ  RN+A 
Sbjct: 552  SLPNTMQTQQYQIGNPAITNEYGRFPSVPRHITRTPVAIQALPARTSNSVIQQNPRNSAN 611

Query: 885  -----------------------RRANLHQVSQMSSSPLHQYSGVQNPGQENRSFPTVRP 995
                                    R+N H VS MSSSPL Q+  +    Q++ SFP+ R 
Sbjct: 612  TIIANGLSAASHASPATPNFPATHRSNPHPVSSMSSSPLLQHPRL----QQSSSFPSARS 667

Query: 996  PHQNVVLQATNQVPDAQRVLNERQLSSQQMANLMMPRAMGQPPAMSQPSYSMNPNGRRMP 1175
            P QN   Q  NQVP   R  N+R  +S QMANL   + M Q P +   S     +  R  
Sbjct: 668  PQQNSGFQDPNQVPSMYRFSNDRDSTSPQMANLRPLQPMSQSPGLGPSSIQSPGSFLRAQ 727

Query: 1176 SVVGDQWS----NVGVAS------------------------------------------ 1217
            + VG          GVAS                                          
Sbjct: 728  NHVGVSQERSVHRTGVASTHQAQLIAAANRTVQMAVDPSRTTLSFSRNTDGRSMSSVDDQ 787

Query: 1218 --------PPLMTTDAYDPAQQTWRPAGRMRGALSGQAYADAYNRFMSQHNQQAAQAARP 1373
                     P+  TDAYDPA   WRPAGRMRGALSGQAYADA N+F+ +  QQ AQAARP
Sbjct: 788  RGNIGVASQPVTRTDAYDPADMNWRPAGRMRGALSGQAYADALNQFIIRPTQQ-AQAARP 846

Query: 1374 ISNVHLDNVNVPAESFM---PPNYPSARPASLP 1463
              NV   NV    ++FM       PSA P++ P
Sbjct: 847  TMNV--ANVAAQLQAFMATPATTNPSAGPSTRP 877


>ref|XP_022880892.1| E4 SUMO-protein ligase PIAL2-like [Olea europaea var. sylvestris]
          Length = 854

 Score =  421 bits (1082), Expect = e-135
 Identities = 265/586 (45%), Positives = 326/586 (55%), Gaps = 99/586 (16%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            +CPIS  RIKTP+KGH CKH+QCFD+DNYV+INSRRPSWRCPHCNQ+VCFTDIR+DQ +V
Sbjct: 276  SCPISLTRIKTPIKGHSCKHLQCFDYDNYVNINSRRPSWRCPHCNQYVCFTDIRLDQIMV 335

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTGD---------GESPQPVD 335
            KVLK+V  N  ++IISSDGSWNAVMD+D A ++PE+K  + G          G +   V+
Sbjct: 336  KVLKDVGPNAKEVIISSDGSWNAVMDTDDATKEPEEKTFNVGQDVPRGIESAGLADASVE 395

Query: 336  LLDLTQTNDGMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQRSAHVEDD 515
            +LDLT+ +D  D V T E+EDRKLS +  Q+ S+TQT SV P +ANT++VNQ  A  +DD
Sbjct: 396  ILDLTEIDDVTDAVATRESEDRKLSPTAHQSLSITQTPSVNPQMANTNDVNQNIAQTDDD 455

Query: 516  FWSGIFLSSLGQGPSNEQIGGVFVPTSNYIASPPVLTDSFTTNGQVEGFNGNNLFTASVP 695
            FWSGIFLS+   G            T N  ++PP+ +    +N +++ F GN   T  V 
Sbjct: 456  FWSGIFLSTYEMG------------TLNVRSNPPITS---VSNWELDAFQGNIPLTTFVM 500

Query: 696  QREPSLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQALPAQTSTSILQQRSR 875
            + E +LPN  QLQQ+QFGN N   EYG  PS+  + TRTP A+QA P Q  TS+LQQRSR
Sbjct: 501  ESETALPNISQLQQYQFGNPNMGNEYGELPSISSHATRTPIAIQAFPTQPPTSVLQQRSR 560

Query: 876  N------------------TARR-----ANLH----QVSQMSSSPLHQYSGVQNPGQENR 974
            N                  +AR      + LH    QVS+M SS + QY G+    Q+NR
Sbjct: 561  NNMDVFIQNSPSAASQASPSARLFADGFSTLHSTSSQVSRMPSSSVQQYLGI----QQNR 616

Query: 975  SFPTVRPPHQNVVLQATNQVPDAQRVLNERQLSSQ-QMANLMMPRAMGQPPAM------- 1130
            S P V P   N+ LQ  NQVP+A RV  ERQ SSQ QMANL    A+ QP          
Sbjct: 617  SVPPVLPSQHNIGLQPPNQVPNAYRVSYERQSSSQLQMANLAASHAINQPFGQVQSSIQP 676

Query: 1131 --------------------------SQPSYSMNPNGRRMP------------------- 1175
                                      SQ S  M PN R  P                   
Sbjct: 677  SANFLGPQNHGVSSRSGVDRSTGIVGSQQSRLMVPNQRNGPMARRIETSRAHPYSLNADA 736

Query: 1176 ---SVVGDQWSNVGVASPPLMTTDAYDPAQQTWRPAGRMRGALSGQAYADAYNRFMSQHN 1346
               S V  Q  N  V S  +  TD  D A Q WRP  RMRGALSGQAY+DA N+F+ +  
Sbjct: 737  HRTSSVAHQIENTEVTSLSVRRTDTRDQADQNWRPTARMRGALSGQAYSDALNQFILRPT 796

Query: 1347 QQAAQAARPISN-VHL-DNVNVPAESFMP-----PNYPSARPASLP 1463
             Q  QAA+P SN V L  NV VP +  M      PNYPS   AS P
Sbjct: 797  PQ-PQAAQPSSNAVSLPGNVPVPLQPLMAQGPQLPNYPSIGQASRP 841


>ref|XP_012834292.1| PREDICTED: E3 SUMO-protein ligase SIZ1 [Erythranthe guttata]
          Length = 843

 Score =  385 bits (988), Expect = e-121
 Identities = 241/535 (45%), Positives = 305/535 (57%), Gaps = 57/535 (10%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCPISF RIKTPVKGH CKHIQCFDFDN+VDINSRRPSWRCPHCNQ+VCF DIRIDQ +V
Sbjct: 323  NCPISFTRIKTPVKGHSCKHIQCFDFDNFVDINSRRPSWRCPHCNQYVCFPDIRIDQNIV 382

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPE-DKISDTGDGESPQPVDLLDLTQTN 359
            KVLKEV  NVSDII+SSD SWNAV +++   +KPE D    TG   SPQP D++DLTQT 
Sbjct: 383  KVLKEVGPNVSDIIVSSDWSWNAVNETEDTTKKPEEDNTFSTGHDSSPQPADVMDLTQTE 442

Query: 360  DGM-DFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDFWSGIFL 536
            D M  F T   TED K      Q+Q +TQT +V     NT++ NQ    + ++FWS + +
Sbjct: 443  DAMVAFPTRETTEDVKHFPDARQSQPLTQTAAV-----NTNDANQTGGPLANNFWSDMLM 497

Query: 537  SSLGQGPSNEQIGGVFVPTSNYIASPPVLTDSFT-TNGQVEGFNGNNLFTASVPQREPSL 713
            S+    PSN +      P +    + P+  D+FT TN ++E F+G+N    +      S 
Sbjct: 498  STFVP-PSNVR------PNAQIGGNIPI--DTFTNTNRELEAFHGSNNALDTT-----SA 543

Query: 714  PNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQALPAQTSTSILQQRSRN----- 878
             NTL LQQ+QF N+    +YGRF S PR++TR P AVQALPAQ+ TS+LQ+ S N     
Sbjct: 544  ANTLPLQQYQFRNTPVNNDYGRFQSAPRHITRVPNAVQALPAQSPTSVLQRGSVNSHNSF 603

Query: 879  -------------TARRANLHQVSQMSSSPLHQYSGVQNPGQENRSFPTVRPPHQNVVLQ 1019
                         TA RA+ HQV  +SSS L     +Q+  Q+NRSF + +PP QN   Q
Sbjct: 604  TPNSLSTTAPNFSTASRAHPHQVPPVSSSSL-----LQHIQQQNRSFSSAQPPQQNAGFQ 658

Query: 1020 ATNQVPDAQRVLNERQLSSQQMANLMMPRAMGQPPAMSQPSYSMN--------------- 1154
             +NQV +  R+ N     SQQ+ANL M R M QP   S  S+                  
Sbjct: 659  DSNQVQNTYRLPNGHN-QSQQIANLRMSRTMSQPSMQSSGSFVRTQTHAGGAPQTIHQTA 717

Query: 1155 --PNGRRMPSVVGDQ-------------WSNVGVASPPLMTTDAYDP-AQQTWRPAGRMR 1286
              PN  +   ++                  + GVA  P+  +DA DP A  TWRPAGRMR
Sbjct: 718  ALPNNSQQSHLIAAANRAAHMAFDPSRTAPSSGVAPQPITRSDASDPTADMTWRPAGRMR 777

Query: 1287 GALSGQAYADAYNRFMSQHNQQAAQAARPI-----SNVHLDNVNVPAESFMPPNY 1436
            GAL GQ Y +AY +++ + NQQA QA  PI     S     N N  +  F P N+
Sbjct: 778  GALVGQDYNNAYKQYVIRPNQQAHQAGNPIVPNQQSQAVRPNSNAGSVQFRPVNW 832


>ref|XP_022867111.1| E4 SUMO-protein ligase PIAL1-like isoform X1 [Olea europaea var.
            sylvestris]
 ref|XP_022867112.1| E4 SUMO-protein ligase PIAL1-like isoform X2 [Olea europaea var.
            sylvestris]
          Length = 525

 Score =  338 bits (866), Expect = e-107
 Identities = 227/533 (42%), Positives = 287/533 (53%), Gaps = 104/533 (19%)
 Frame = +3

Query: 177  LVKVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTGD---------GESPQP 329
            + KVLKEV  NV ++IISSDGSWNAV++++   +KPEDK S+ G          G    P
Sbjct: 1    MAKVLKEVGPNVKEVIISSDGSWNAVIETEDTTKKPEDKTSNVGHDAPSEIESTGLPGAP 60

Query: 330  VDLLDLTQTNDGMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQRSAHVE 509
            V++LDLT+ +DGMD V T E+ED KLS +  Q+ SMTQT SV P +ANT +VNQ    ++
Sbjct: 61   VEILDLTEIDDGMDAVATRESEDTKLSPTACQSLSMTQTPSVNPQMANTCDVNQNIGQID 120

Query: 510  DDFWSGIFLSSLGQGPSNEQIGGVFVPTSNYIASPPVLTDSFTTNGQVEGFNGNNLFTAS 689
            +DFWSGI++S+ G G            TSN  ++ P+   +  ++ +++ F+GN   + S
Sbjct: 121  EDFWSGIYMSTFGTG------------TSNVRSNLPI---AGVSSRELDAFHGNVPLSTS 165

Query: 690  VPQREPSLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQALPAQTSTSILQQR 869
            VP+ E +LPN LQLQQ+QFGNSN + EYGR PS+  +VTRTP A+QALPAQ   S+LQQR
Sbjct: 166  VPESETALPNILQLQQYQFGNSNMSNEYGRLPSISSHVTRTPIAIQALPAQAPASVLQQR 225

Query: 870  SRNTARR---------------------------ANLHQVSQMSSSPLHQYSGVQNPGQE 968
            SRN+                              ++  QVSQM SS L QYSG+    Q+
Sbjct: 226  SRNSTNTFMQNNPSAAAQASPSARLVPDGFSMLDSSSSQVSQMPSSSLQQYSGI----QQ 281

Query: 969  NRSFPTVRPPHQNVVLQATNQVPDAQRVLNERQLSSQ-QMANLMMPRAMGQ--------- 1118
            NRS P+VRP   N+ LQA + VP+A RV NERQ +SQ QMANL    AM Q         
Sbjct: 282  NRSVPSVRPSQHNIGLQAPHLVPNAYRVSNERQSTSQLQMANLAASHAMNQSFGQVQSSI 341

Query: 1119 -------PPAM-----------------SQPSYSMNPN---------------------- 1160
                    P M                 SQ S  M PN                      
Sbjct: 342  LPSANFLSPQMHGVGSQSGVDRSTGVIGSQQSRLMVPNQRNGQMARPVETSRAHTYSLNP 401

Query: 1161 -GRRMPSVVGDQWSNVGVASPPLMTTDAYDPAQQTWRPAGRMRGALSGQAYADAYNRFMS 1337
              RRMPS V  Q  N G  S  +  TDAYD A Q WRP  RMRGALSGQAY+DA N+F+ 
Sbjct: 402  DARRMPS-VSHQIENTGGTSVTVPRTDAYDQADQNWRPTARMRGALSGQAYSDALNQFIL 460

Query: 1338 QHNQQAAQAARPISNVHL--DNVNVP---------AESFMPPNYPSARPASLP 1463
            +   Q  QAARPISN      NV  P         A+S   PN+PS   AS P
Sbjct: 461  RPTSQ-PQAARPISNAGSLPGNVRAPLQPHMADRVAQSPQLPNHPSIGQASRP 512


>ref|XP_019247404.1| PREDICTED: E4 SUMO-protein ligase PIAL1-like isoform X3 [Nicotiana
            attenuata]
          Length = 782

 Score =  331 bits (849), Expect = e-102
 Identities = 220/562 (39%), Positives = 290/562 (51%), Gaps = 75/562 (13%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCPISF+RIKTPVKGH CKH+QCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQ + 
Sbjct: 219  NCPISFKRIKTPVKGHSCKHLQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQDMD 278

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISD--------TGDGESPQPVDL 338
            KVLKEV  +V+D++ISSDGSW A+M+SD   +KP DKI D          D  S  P D+
Sbjct: 279  KVLKEVGEDVTDVMISSDGSWKAIMESDDHSEKPRDKIPDFTQDSPQRGSDSFSNTPADV 338

Query: 339  LDLTQTNDGMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDF 518
            +DLT  +D ++ + T+ETED K   SI    S  Q  +V  + +  D+     + + DDF
Sbjct: 339  MDLTAIDDEINPMATSETEDSKNFPSIPNIHSNVQNTTVVNNPSEIDHTG--GSDMADDF 396

Query: 519  WSGIFLSSLGQGPSNE----QIGGVFVPTSNYIASPPVLTDSFTTNGQVEGFNGNNLFTA 686
            WS +++SS G G S      Q G    P+   +  PPVLTD+ +     E   GN L   
Sbjct: 397  WSRMYMSSCGIGTSGSLSSLQNGSASEPSRTNLLQPPVLTDAISPAFNPE---GNALIPT 453

Query: 687  SVPQREPSLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQ---ALPAQTSTSI 857
            S+ +   S  + +QLQQFQFGNS  + EYGRFPS  R+  RTP AVQ   ALPAQ  T +
Sbjct: 454  SILESGLSSSDMMQLQQFQFGNSAISNEYGRFPSAARHANRTPVAVQALSALPAQVQTPV 513

Query: 858  LQQRSRNTARRANLHQ------------------------------------VSQMSSSP 929
             Q+  + +A    LH                                      ++M+SS 
Sbjct: 514  PQR--QQSAMNPLLHTGPSVATQGLPTVSLDGSNVRSDLERQQCSVPDLDLLQARMTSSA 571

Query: 930  LHQYSGVQNPGQENRSFPTVRPPHQNVVL-----------------QATNQVPDAQRVLN 1058
            L Q    Q  G++  +  T  P  Q+  L                 Q   +        +
Sbjct: 572  LPQKVSAQVVGRQTPNLRTPYPMSQSEGLTRQATLDRWEALKKGSSQGVTRATGLAGGQH 631

Query: 1059 ERQLSSQQMANLMMPRAMGQPPAMSQPSYSMNPNGRRMPSVVGDQWSNVGVASPPLMTTD 1238
             R +++QQ   ++ P    Q P  + P    N +G R P +  DQ  + G  +P   T  
Sbjct: 632  TRVVATQQTTQVVRP---VQTPRTASPVLPGNSDGFRTP-LARDQRGSTGGTTPVTRTDS 687

Query: 1239 AYD-PAQQTWRPAGRMRGALSGQAYADAYNRFMSQHNQQAAQAARPISNVHLDN------ 1397
            + D      WRP GRMRG+LSG+AY++A N+++ Q  QQ  QA RP    +L        
Sbjct: 688  SGDVQLDPNWRPTGRMRGSLSGRAYSEALNQYILQPTQQ-TQAPRPSVPPNLSPQLQVFL 746

Query: 1398 VNVPAESFMPPNYPSARPASLP 1463
             N  A S  P N+PS  PA+ P
Sbjct: 747  ANRGAHSTQPVNHPSTTPANAP 768


>ref|XP_019247402.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X2 [Nicotiana
            attenuata]
          Length = 789

 Score =  329 bits (844), Expect = e-101
 Identities = 223/569 (39%), Positives = 296/569 (52%), Gaps = 82/569 (14%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCPISF+RIKTPVKGH CKH+QCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQ + 
Sbjct: 219  NCPISFKRIKTPVKGHSCKHLQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQDMD 278

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISD--------TGDGESPQPVDL 338
            KVLKEV  +V+D++ISSDGSW A+M+SD   +KP DKI D          D  S  P D+
Sbjct: 279  KVLKEVGEDVTDVMISSDGSWKAIMESDDHSEKPRDKIPDFTQDSPQRGSDSFSNTPADV 338

Query: 339  LDLTQTNDGMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDF 518
            +DLT  +D ++ + T+ETED K   SI    S  Q  +V  + +  D+     + + DDF
Sbjct: 339  MDLTAIDDEINPMATSETEDSKNFPSIPNIHSNVQNTTVVNNPSEIDHTG--GSDMADDF 396

Query: 519  WSGIFLSSLGQGPSNE----QIGGVFVPTSNYIASPPVLTDSFTTNGQVEGFNGNNLFTA 686
            WS +++SS G G S      Q G    P+   +  PPVLTD+ +     E   GN L   
Sbjct: 397  WSRMYMSSCGIGTSGSLSSLQNGSASEPSRTNLLQPPVLTDAISPAFNPE---GNALIPT 453

Query: 687  SVPQREPSLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQ---ALPAQTSTSI 857
            S+ +   S  + +QLQQFQFGNS  + EYGRFPS  R+  RTP AVQ   ALPAQ  T +
Sbjct: 454  SILESGLSSSDMMQLQQFQFGNSAISNEYGRFPSAARHANRTPVAVQALSALPAQVQTPV 513

Query: 858  LQQRSRNTARRANLHQ------------------------------------VSQMSSSP 929
             Q+  + +A    LH                                      ++M+SS 
Sbjct: 514  PQR--QQSAMNPLLHTGPSVATQGLPTVSLDGSNVRSDLERQQCSVPDLDLLQARMTSSA 571

Query: 930  LHQYSGVQ--NPGQE--NRSFPTVRPPH-----QNVVLQATNQVPDAQRV---------- 1052
            L Q   +    P Q+   R  P +R P+     + +  QAT    +A +           
Sbjct: 572  LPQKRSLAPLQPSQQVVGRQTPNLRTPYPMSQSEGLTRQATLDRWEALKKGSSQGVTRAT 631

Query: 1053 -----LNERQLSSQQMANLMMPRAMGQPPAMSQPSYSMNPNGRRMPSVVGDQWSNVGVAS 1217
                  + R +++QQ   ++ P    Q P  + P    N +G R P +  DQ  + G  +
Sbjct: 632  GLAGGQHTRVVATQQTTQVVRP---VQTPRTASPVLPGNSDGFRTP-LARDQRGSTGGTT 687

Query: 1218 PPLMTTDAYD-PAQQTWRPAGRMRGALSGQAYADAYNRFMSQHNQQAAQAARPISNVHLD 1394
            P   T  + D      WRP GRMRG+LSG+AY++A N+++ Q  QQ  QA RP    +L 
Sbjct: 688  PVTRTDSSGDVQLDPNWRPTGRMRGSLSGRAYSEALNQYILQPTQQ-TQAPRPSVPPNLS 746

Query: 1395 N------VNVPAESFMPPNYPSARPASLP 1463
                    N  A S  P N+PS  PA+ P
Sbjct: 747  PQLQVFLANRGAHSTQPVNHPSTTPANAP 775


>ref|XP_019247401.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X1 [Nicotiana
            attenuata]
 gb|OIT02146.1| e4 sumo-protein ligase pial2 [Nicotiana attenuata]
          Length = 793

 Score =  329 bits (844), Expect = e-101
 Identities = 224/573 (39%), Positives = 296/573 (51%), Gaps = 86/573 (15%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCPISF+RIKTPVKGH CKH+QCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQ + 
Sbjct: 219  NCPISFKRIKTPVKGHSCKHLQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQDMD 278

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISD--------TGDGESPQPVDL 338
            KVLKEV  +V+D++ISSDGSW A+M+SD   +KP DKI D          D  S  P D+
Sbjct: 279  KVLKEVGEDVTDVMISSDGSWKAIMESDDHSEKPRDKIPDFTQDSPQRGSDSFSNTPADV 338

Query: 339  LDLTQTNDGMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDF 518
            +DLT  +D ++ + T+ETED K   SI    S  Q  +V  + +  D+     + + DDF
Sbjct: 339  MDLTAIDDEINPMATSETEDSKNFPSIPNIHSNVQNTTVVNNPSEIDHTG--GSDMADDF 396

Query: 519  WSGIFLSSLGQGPSNE----QIGGVFVPTSNYIASPPVLTDSFTTNGQVEGFNGNNLFTA 686
            WS +++SS G G S      Q G    P+   +  PPVLTD+ +     E   GN L   
Sbjct: 397  WSRMYMSSCGIGTSGSLSSLQNGSASEPSRTNLLQPPVLTDAISPAFNPE---GNALIPT 453

Query: 687  SVPQREPSLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQ---ALPAQTSTSI 857
            S+ +   S  + +QLQQFQFGNS  + EYGRFPS  R+  RTP AVQ   ALPAQ  T +
Sbjct: 454  SILESGLSSSDMMQLQQFQFGNSAISNEYGRFPSAARHANRTPVAVQALSALPAQVQTPV 513

Query: 858  LQQRSRNTARRANLHQ------------------------------------VSQMSSSP 929
             Q+  + +A    LH                                      ++M+SS 
Sbjct: 514  PQR--QQSAMNPLLHTGPSVATQGLPTVSLDGSNVRSDLERQQCSVPDLDLLQARMTSSA 571

Query: 930  LHQYSGVQ------NPGQE--NRSFPTVRPPH-----QNVVLQATNQVPDAQRV------ 1052
            L Q    Q       P Q+   R  P +R P+     + +  QAT    +A +       
Sbjct: 572  LPQKVSAQRSLAPLQPSQQVVGRQTPNLRTPYPMSQSEGLTRQATLDRWEALKKGSSQGV 631

Query: 1053 ---------LNERQLSSQQMANLMMPRAMGQPPAMSQPSYSMNPNGRRMPSVVGDQWSNV 1205
                      + R +++QQ   ++ P    Q P  + P    N +G R P +  DQ  + 
Sbjct: 632  TRATGLAGGQHTRVVATQQTTQVVRP---VQTPRTASPVLPGNSDGFRTP-LARDQRGST 687

Query: 1206 GVASPPLMTTDAYD-PAQQTWRPAGRMRGALSGQAYADAYNRFMSQHNQQAAQAARPISN 1382
            G  +P   T  + D      WRP GRMRG+LSG+AY++A N+++ Q  QQ  QA RP   
Sbjct: 688  GGTTPVTRTDSSGDVQLDPNWRPTGRMRGSLSGRAYSEALNQYILQPTQQ-TQAPRPSVP 746

Query: 1383 VHLDN------VNVPAESFMPPNYPSARPASLP 1463
             +L         N  A S  P N+PS  PA+ P
Sbjct: 747  PNLSPQLQVFLANRGAHSTQPVNHPSTTPANAP 779


>ref|XP_019247405.1| PREDICTED: E4 SUMO-protein ligase PIAL1-like isoform X4 [Nicotiana
            attenuata]
          Length = 778

 Score =  328 bits (840), Expect = e-100
 Identities = 219/559 (39%), Positives = 288/559 (51%), Gaps = 72/559 (12%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCPISF+RIKTPVKGH CKH+QCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQ + 
Sbjct: 219  NCPISFKRIKTPVKGHSCKHLQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQDMD 278

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISD--------TGDGESPQPVDL 338
            KVLKEV  +V+D++ISSDGSW A+M+SD   +KP DKI D          D  S  P D+
Sbjct: 279  KVLKEVGEDVTDVMISSDGSWKAIMESDDHSEKPRDKIPDFTQDSPQRGSDSFSNTPADV 338

Query: 339  LDLTQTNDGMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDF 518
            +DLT  +D ++ + T+ETED K   SI    S  Q  +V  + +  D+     + + DDF
Sbjct: 339  MDLTAIDDEINPMATSETEDSKNFPSIPNIHSNVQNTTVVNNPSEIDHTG--GSDMADDF 396

Query: 519  WSGIFLSSLGQGPSNE----QIGGVFVPTSNYIASPPVLTDSFTTNGQVEGFNGNNLFTA 686
            WS +++SS G G S      Q G    P+   +  PPVLTD+ +     E   GN L   
Sbjct: 397  WSRMYMSSCGIGTSGSLSSLQNGSASEPSRTNLLQPPVLTDAISPAFNPE---GNALIPT 453

Query: 687  SVPQREPSLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQ---ALPAQTSTSI 857
            S+ +   S  + +QLQQFQFGNS  + EYGRFPS  R+  RTP AVQ   ALPAQ  T +
Sbjct: 454  SILESGLSSSDMMQLQQFQFGNSAISNEYGRFPSAARHANRTPVAVQALSALPAQVQTPV 513

Query: 858  LQ-----------------------------------QRSRNTARRANLHQVSQMSSSPL 932
             Q                                   +R + +    +L Q    SS+  
Sbjct: 514  PQRQQSAMNPLLHTGPSVATQGLPTVSLDGSNVRSDLERQQCSVPDLDLLQARMTSSALP 573

Query: 933  HQYSGVQNPGQENRSFPTVRPPHQNVVLQATNQVPDAQRV---------------LNERQ 1067
             +  G Q P   N   P      + +  QAT    +A +                 + R 
Sbjct: 574  QKVVGRQTP---NLRTPYPMSQSEGLTRQATLDRWEALKKGSSQGVTRATGLAGGQHTRV 630

Query: 1068 LSSQQMANLMMPRAMGQPPAMSQPSYSMNPNGRRMPSVVGDQWSNVGVASPPLMTTDAYD 1247
            +++QQ   ++ P    Q P  + P    N +G R P +  DQ  + G  +P   T  + D
Sbjct: 631  VATQQTTQVVRP---VQTPRTASPVLPGNSDGFRTP-LARDQRGSTGGTTPVTRTDSSGD 686

Query: 1248 -PAQQTWRPAGRMRGALSGQAYADAYNRFMSQHNQQAAQAARPISNVHLDN------VNV 1406
                  WRP GRMRG+LSG+AY++A N+++ Q  QQ  QA RP    +L         N 
Sbjct: 687  VQLDPNWRPTGRMRGSLSGRAYSEALNQYILQPTQQ-TQAPRPSVPPNLSPQLQVFLANR 745

Query: 1407 PAESFMPPNYPSARPASLP 1463
             A S  P N+PS  PA+ P
Sbjct: 746  GAHSTQPVNHPSTTPANAP 764


>ref|XP_016454176.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X2 [Nicotiana
            tabacum]
          Length = 890

 Score =  328 bits (842), Expect = e-99
 Identities = 226/568 (39%), Positives = 298/568 (52%), Gaps = 81/568 (14%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCPISF+RIKTPVKGH CKH+QCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQ + 
Sbjct: 320  NCPISFKRIKTPVKGHSCKHLQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQDMD 379

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISD--------TGDGESPQPVDL 338
            KVLKEV  +V+D++ISSDGSW A+M+SD   +K  DKI D          DG S  P D+
Sbjct: 380  KVLKEVGEDVTDVMISSDGSWKAIMESDDHTEKSRDKIPDFTQDSPQRGSDGFSNTPADV 439

Query: 339  LDLTQTNDGMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQR-SAHVEDD 515
            +DLT  +D ++ + T+ETED K   SI    S  Q  +V   + N   +N    + + DD
Sbjct: 440  MDLTDIDDEINPMATSETEDSKNFPSIPNIHSNGQNTTV---VNNPSEINHTGGSDMADD 496

Query: 516  FWSGIFLSSLGQGPSNE----QIGGVFVPTSNYIASPPVLTDSFTTNGQVEGFNGNNLFT 683
            FWS +++SS G G S      Q G    P+   +  PPVLTD+ +     E   GN L  
Sbjct: 497  FWSRMYMSSCGIGTSGSLSSLQNGSASEPSRTNLLQPPVLTDAISPALNPE---GNALIP 553

Query: 684  ASVPQREPSLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQALPA-----QTS 848
             S+ +   S  + +QLQQFQFGNS  + EYGRFPS  R+  RTP AVQALPA     QT 
Sbjct: 554  TSILECGLSSSDLMQLQQFQFGNSAISNEYGRFPSAVRHANRTPVAVQALPALPAQMQTL 613

Query: 849  TSILQQRSRN---------------------TARRANLHQ-----------VSQMSSSPL 932
                QQ + N                     +  R++L +            ++M+SS L
Sbjct: 614  VPQRQQSAMNPLLHTGPSVATQGLPTVSLDGSNVRSDLERQRCFVPDLDLLQARMTSSAL 673

Query: 933  HQYSGVQ--NPGQE--NRSFPTVRPPH-----QNVVLQATNQVPDAQRV----------- 1052
             Q   +    P Q+      P +R P+     Q +  QAT    +A +            
Sbjct: 674  PQKRSLAPLQPSQQVVGHQTPNLRTPYPMSQSQGLTRQATLDRWEALKKGSSQGVTRTTG 733

Query: 1053 ----LNERQLSSQQMANLMMPRAMGQPPAMSQPSYSMNPNGRRMPSVVGDQWSNVGVASP 1220
                 + R +++QQ   ++ P    Q P  + P    N +G R P +  DQ  + G  +P
Sbjct: 734  LAGGQHTRVVATQQTTQVVRP---VQTPRAASPVLPGNSDGFRTP-LAQDQRGSTGGTTP 789

Query: 1221 PLMTTDAYD-PAQQTWRPAGRMRGALSGQAYADAYNRFMSQHNQQAAQAARPISNVHLDN 1397
               T  + D      WRP GRMRG+LSG+AY++A N+++ Q  QQ AQA RP    +L  
Sbjct: 790  VTRTDSSGDSQLDPNWRPTGRMRGSLSGRAYSEALNQYILQPTQQ-AQAPRPSVPPNLSP 848

Query: 1398 ------VNVPAESFMPPNYPSARPASLP 1463
                   N  A S  P N+PS  PA+ P
Sbjct: 849  QLQVFLANRGAHSTQPVNHPSTTPANAP 876


>ref|XP_016454175.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X1 [Nicotiana
            tabacum]
          Length = 894

 Score =  328 bits (842), Expect = 1e-99
 Identities = 227/572 (39%), Positives = 298/572 (52%), Gaps = 85/572 (14%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCPISF+RIKTPVKGH CKH+QCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQ + 
Sbjct: 320  NCPISFKRIKTPVKGHSCKHLQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQDMD 379

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISD--------TGDGESPQPVDL 338
            KVLKEV  +V+D++ISSDGSW A+M+SD   +K  DKI D          DG S  P D+
Sbjct: 380  KVLKEVGEDVTDVMISSDGSWKAIMESDDHTEKSRDKIPDFTQDSPQRGSDGFSNTPADV 439

Query: 339  LDLTQTNDGMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQR-SAHVEDD 515
            +DLT  +D ++ + T+ETED K   SI    S  Q  +V   + N   +N    + + DD
Sbjct: 440  MDLTDIDDEINPMATSETEDSKNFPSIPNIHSNGQNTTV---VNNPSEINHTGGSDMADD 496

Query: 516  FWSGIFLSSLGQGPSNE----QIGGVFVPTSNYIASPPVLTDSFTTNGQVEGFNGNNLFT 683
            FWS +++SS G G S      Q G    P+   +  PPVLTD+ +     E   GN L  
Sbjct: 497  FWSRMYMSSCGIGTSGSLSSLQNGSASEPSRTNLLQPPVLTDAISPALNPE---GNALIP 553

Query: 684  ASVPQREPSLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQALPA-----QTS 848
             S+ +   S  + +QLQQFQFGNS  + EYGRFPS  R+  RTP AVQALPA     QT 
Sbjct: 554  TSILECGLSSSDLMQLQQFQFGNSAISNEYGRFPSAVRHANRTPVAVQALPALPAQMQTL 613

Query: 849  TSILQQRSRN---------------------TARRANLHQ-----------VSQMSSSPL 932
                QQ + N                     +  R++L +            ++M+SS L
Sbjct: 614  VPQRQQSAMNPLLHTGPSVATQGLPTVSLDGSNVRSDLERQRCFVPDLDLLQARMTSSAL 673

Query: 933  HQYSGVQ------NPGQE--NRSFPTVRPPH-----QNVVLQATNQVPDAQRV------- 1052
             Q    Q       P Q+      P +R P+     Q +  QAT    +A +        
Sbjct: 674  PQKVSAQRSLAPLQPSQQVVGHQTPNLRTPYPMSQSQGLTRQATLDRWEALKKGSSQGVT 733

Query: 1053 --------LNERQLSSQQMANLMMPRAMGQPPAMSQPSYSMNPNGRRMPSVVGDQWSNVG 1208
                     + R +++QQ   ++ P    Q P  + P    N +G R P +  DQ  + G
Sbjct: 734  RTTGLAGGQHTRVVATQQTTQVVRP---VQTPRAASPVLPGNSDGFRTP-LAQDQRGSTG 789

Query: 1209 VASPPLMTTDAYD-PAQQTWRPAGRMRGALSGQAYADAYNRFMSQHNQQAAQAARPISNV 1385
              +P   T  + D      WRP GRMRG+LSG+AY++A N+++ Q  QQ AQA RP    
Sbjct: 790  GTTPVTRTDSSGDSQLDPNWRPTGRMRGSLSGRAYSEALNQYILQPTQQ-AQAPRPSVPP 848

Query: 1386 HLDN------VNVPAESFMPPNYPSARPASLP 1463
            +L         N  A S  P N+PS  PA+ P
Sbjct: 849  NLSPQLQVFLANRGAHSTQPVNHPSTTPANAP 880


>ref|XP_016552263.1| PREDICTED: E4 SUMO-protein ligase PIAL2 [Capsicum annuum]
          Length = 856

 Score =  327 bits (837), Expect = 3e-99
 Identities = 223/550 (40%), Positives = 282/550 (51%), Gaps = 65/550 (11%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCPISFRRIKTPVKGH CKH+QCFDFDNY DINSRRPSWRCPHCNQ+VCFTDIRIDQ +V
Sbjct: 318  NCPISFRRIKTPVKGHSCKHLQCFDFDNYTDINSRRPSWRCPHCNQYVCFTDIRIDQDMV 377

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDT--------GDGESPQPVDL 338
            K+LKEV  +V+D++ISSDGSW AVM+SD   +KP DK  D          DG S  P D+
Sbjct: 378  KILKEVSEDVTDVVISSDGSWKAVMESDDRTEKPRDKTPDIAQDSPHRGSDGPSNAPSDV 437

Query: 339  LDLTQTNDGMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQRSAHVEDDF 518
            LDLT+ +D M  VTT ETEDRK   +ISQ QS  Q+ +V   + N   +NQ    + DDF
Sbjct: 438  LDLTEMDDEMYPVTTAETEDRKNFPAISQIQSNVQSTTV---VNNPSELNQTGPGMTDDF 494

Query: 519  WSGIFLSSLGQGPSNEQIGGVFVPTSNYIASPPVLTDSFTTNGQVEGFNGNNLFTASVPQ 698
            WS ++LSSL  G ++E       P    +   PV TD+             ++   +V +
Sbjct: 495  WSRLYLSSLQTGSASE-------PARINLLQLPVFTDAIPP---------TSIIPTAVLE 538

Query: 699  REPSLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQALPAQTSTSILQQRSRN 878
             E +  N LQLQQFQFGNS  + EYGRF S  R   RTP AVQALPAQ +  +  QR + 
Sbjct: 539  NELAASNMLQLQQFQFGNSAISNEYGRFASSGRQANRTPIAVQALPAQMNPPVSHQR-QQ 597

Query: 879  TARRANLHQVSQMSSSPLHQYSGVQNPGQENRSFPTVRPPHQNVVLQATNQVPDAQRVLN 1058
            +A    LH    +++  L         G    SF  +     ++   A  Q   +QR L 
Sbjct: 598  SAMNPLLHAGPSVTTRDLPSVL----DGSNLNSFADLDLLQAHMTSSALPQRVSSQRPLP 653

Query: 1059 ERQLS----SQQMANLMMPRAMGQPPAMSQPSYS------------------MNPNGRRM 1172
              Q S     +Q  N   P +M QP  ++Q + +                   +P G+  
Sbjct: 654  HLQPSPHVVGRQHPNTRTPSSMSQPQGLTQSAAAWDRWEAMKQGSSQAGVSRAHPVGQNA 713

Query: 1173 PSVVGDQWSNV-------GVASP----------PLM-----------TTDAYDP-AQQTW 1265
              V   Q + V         ASP          PL+           T    DP     W
Sbjct: 714  RVVATQQNTQVVRPVQPPRTASPVSGSADRFRTPLVPDHRGSMGGTPTESPVDPQLDPNW 773

Query: 1266 RPAGRMRGALSGQAYADAYNRFMSQHNQQAAQAARPISNVHLDN------VNVPAESFMP 1427
            RP GRMRG+LSG+AY++A    + Q  QQ AQAARP    +L         N  A S  P
Sbjct: 774  RPTGRMRGSLSGRAYSEALQTLIVQPTQQ-AQAARPSIPPNLSPQLQVLLANRGAHSTQP 832

Query: 1428 PNYPSARPAS 1457
             N  S  P +
Sbjct: 833  VNLSSTAPTN 842


>ref|XP_009612350.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X2 [Nicotiana
            tomentosiformis]
          Length = 890

 Score =  327 bits (839), Expect = 3e-99
 Identities = 225/568 (39%), Positives = 298/568 (52%), Gaps = 81/568 (14%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCPISF+RIKTPVKGH CKH+QCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQ + 
Sbjct: 320  NCPISFKRIKTPVKGHSCKHLQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQDMD 379

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISD--------TGDGESPQPVDL 338
            KVLKEV  +V+D++ISSDGSW A+M+SD   +K  DKI D          DG S  P D+
Sbjct: 380  KVLKEVGEDVTDVMISSDGSWKAIMESDDHTEKSRDKIPDFTQDSPQRGSDGFSNTPADV 439

Query: 339  LDLTQTNDGMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQR-SAHVEDD 515
            +DLT  +D ++ + T+ETED K   SI    S  Q  +V   + N   +N    + + DD
Sbjct: 440  MDLTDIDDEINPMATSETEDSKNFPSIPNIHSNGQNTTV---VNNPSEINHTGGSDMADD 496

Query: 516  FWSGIFLSSLGQGPSNE----QIGGVFVPTSNYIASPPVLTDSFTTNGQVEGFNGNNLFT 683
            FWS +++SS G G S      Q G    P+   +  PPVLTD+ +     E   GN L  
Sbjct: 497  FWSRMYMSSCGIGTSGSLSSLQNGSASEPSRTNLLQPPVLTDAISPALNPE---GNALIP 553

Query: 684  ASVPQREPSLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQALPA-----QTS 848
             S+ +   S  + +QLQQFQFGNS  + EYGRFPS  R+  RTP AVQALPA     QT 
Sbjct: 554  TSILECGLSSSDLMQLQQFQFGNSAISNEYGRFPSAVRHANRTPVAVQALPALPAQMQTL 613

Query: 849  TSILQQRSRN---------------------TARRANLHQ-----------VSQMSSSPL 932
                QQ + N                     +  R++L +            ++M+SS L
Sbjct: 614  VPQRQQSAMNPLLHTGPSVATQGLPTVSLDGSNVRSDLERQRCFVPDLDLLQARMTSSAL 673

Query: 933  HQYSGVQ--NPGQE--NRSFPTVRPPH-----QNVVLQATNQVPDAQRV----------- 1052
             Q   +    P Q+      P +R P+     Q +  QAT    +A +            
Sbjct: 674  PQKRSLAPLQPSQQVVGHQTPNLRTPYPMSQSQGLTRQATLDRWEALKKGSSQGVTRTTG 733

Query: 1053 ----LNERQLSSQQMANLMMPRAMGQPPAMSQPSYSMNPNGRRMPSVVGDQWSNVGVASP 1220
                 + R +++QQ   ++ P    Q P  + P    N +G R P +  DQ  + G  +P
Sbjct: 734  LAGGQHTRVVATQQTTQVVRP---VQTPRAASPVLPGNSDGFRTP-LAQDQRGSTGGTTP 789

Query: 1221 PLMTTDAYD-PAQQTWRPAGRMRGALSGQAYADAYNRFMSQHNQQAAQAARPISNVHLDN 1397
               T  + D      WRP GRMRG+LSG+AY++A N+++ Q  QQ AQ+ RP    +L  
Sbjct: 790  VTRTDSSGDSQLDPNWRPTGRMRGSLSGRAYSEALNQYILQPTQQ-AQSPRPSVPPNLSP 848

Query: 1398 ------VNVPAESFMPPNYPSARPASLP 1463
                   N  A S  P N+PS  PA+ P
Sbjct: 849  QLQVFLANRGAHSTQPVNHPSTTPANAP 876


>gb|PHT39733.1| hypothetical protein CQW23_18587 [Capsicum baccatum]
          Length = 859

 Score =  327 bits (837), Expect = 3e-99
 Identities = 224/553 (40%), Positives = 281/553 (50%), Gaps = 68/553 (12%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCPISFRRIKTPVKGH CKH+QCFDFDNY DINSRRPSWRCPHCNQHVCFTDIRIDQ +V
Sbjct: 318  NCPISFRRIKTPVKGHSCKHLQCFDFDNYTDINSRRPSWRCPHCNQHVCFTDIRIDQDMV 377

Query: 183  K---VLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDT--------GDGESPQP 329
            K   +LKEV  +V+D++ISSDGSW AVM+SD   +KP DK  D          DG S  P
Sbjct: 378  KASGILKEVSEDVTDVVISSDGSWKAVMESDDRTEKPRDKTPDIAQDSPHRGSDGPSNAP 437

Query: 330  VDLLDLTQTNDGMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQRSAHVE 509
             D+LDLT+ +D M  VTT ETEDRK   +ISQ QS  Q+ +V   + N   +NQ    + 
Sbjct: 438  SDVLDLTEMDDEMYPVTTAETEDRKNFPAISQIQSNVQSTTV---VNNPSEINQTGPGMT 494

Query: 510  DDFWSGIFLSSLGQGPSNEQIGGVFVPTSNYIASPPVLTDSFTTNGQVEGFNGNNLFTAS 689
            DDFWS ++LSSL  G ++E       P    +   PV TD+             ++   +
Sbjct: 495  DDFWSRLYLSSLQTGSASE-------PARINLLQLPVFTDAIPP---------TSIIPTA 538

Query: 690  VPQREPSLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQALPAQTSTSILQQR 869
            V + E +  N LQLQQFQFGNS  + EYGRF S  R   RTP AVQALPAQ +T +  QR
Sbjct: 539  VLENELAASNMLQLQQFQFGNSAISNEYGRFASSGRQANRTPIAVQALPAQMNTPVSHQR 598

Query: 870  SRNTARRANLHQVSQMSSSPLHQYSGVQNPGQENRSFPTVRPPHQNVVLQATNQVPDAQR 1049
             + +A    LH    +++  L         G    SF  +      +   A  Q   AQR
Sbjct: 599  -QQSAMNPLLHAGPSVTTRDLPSVL----DGSNLNSFADLDLLQARMTSPALPQRVSAQR 653

Query: 1050 VLNERQLS----SQQMANLMMPRAMGQPPAMSQPSYS------------------MNPNG 1163
             L   Q S     +Q  N   P +M QP  ++Q + +                   +P G
Sbjct: 654  PLPHLQPSPHVVGRQHPNTRTPSSMSQPQGLTQSAAAWDRWEAMKQGSSQAGVSRAHPVG 713

Query: 1164 RRMPSVVGDQWSNV-------GVASPPLMTTDAY---------------------DP-AQ 1256
            +    V   Q + V         ASP   + D +                     DP   
Sbjct: 714  QNARVVTTQQNTQVVRPVQPPRTASPVSGSADRFRTPLAPDHRGSMGGTPTESPVDPQLD 773

Query: 1257 QTWRPAGRMRGALSGQAYADAYNRFMSQHNQQAAQAARPISNVHLDN------VNVPAES 1418
              WRP GRMRG+LSG+AY++A    + Q  QQ AQAARP    +L         N  A S
Sbjct: 774  PNWRPTGRMRGSLSGRAYSEALQTLIVQPTQQ-AQAARPSIPPNLSPQLQVLLANRGAHS 832

Query: 1419 FMPPNYPSARPAS 1457
              P N  S  P +
Sbjct: 833  TQPVNLSSTAPTN 845


>ref|XP_009612349.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X1 [Nicotiana
            tomentosiformis]
          Length = 894

 Score =  327 bits (839), Expect = 3e-99
 Identities = 226/572 (39%), Positives = 298/572 (52%), Gaps = 85/572 (14%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCPISF+RIKTPVKGH CKH+QCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQ + 
Sbjct: 320  NCPISFKRIKTPVKGHSCKHLQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQDMD 379

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISD--------TGDGESPQPVDL 338
            KVLKEV  +V+D++ISSDGSW A+M+SD   +K  DKI D          DG S  P D+
Sbjct: 380  KVLKEVGEDVTDVMISSDGSWKAIMESDDHTEKSRDKIPDFTQDSPQRGSDGFSNTPADV 439

Query: 339  LDLTQTNDGMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQR-SAHVEDD 515
            +DLT  +D ++ + T+ETED K   SI    S  Q  +V   + N   +N    + + DD
Sbjct: 440  MDLTDIDDEINPMATSETEDSKNFPSIPNIHSNGQNTTV---VNNPSEINHTGGSDMADD 496

Query: 516  FWSGIFLSSLGQGPSNE----QIGGVFVPTSNYIASPPVLTDSFTTNGQVEGFNGNNLFT 683
            FWS +++SS G G S      Q G    P+   +  PPVLTD+ +     E   GN L  
Sbjct: 497  FWSRMYMSSCGIGTSGSLSSLQNGSASEPSRTNLLQPPVLTDAISPALNPE---GNALIP 553

Query: 684  ASVPQREPSLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQALPA-----QTS 848
             S+ +   S  + +QLQQFQFGNS  + EYGRFPS  R+  RTP AVQALPA     QT 
Sbjct: 554  TSILECGLSSSDLMQLQQFQFGNSAISNEYGRFPSAVRHANRTPVAVQALPALPAQMQTL 613

Query: 849  TSILQQRSRN---------------------TARRANLHQ-----------VSQMSSSPL 932
                QQ + N                     +  R++L +            ++M+SS L
Sbjct: 614  VPQRQQSAMNPLLHTGPSVATQGLPTVSLDGSNVRSDLERQRCFVPDLDLLQARMTSSAL 673

Query: 933  HQYSGVQ------NPGQE--NRSFPTVRPPH-----QNVVLQATNQVPDAQRV------- 1052
             Q    Q       P Q+      P +R P+     Q +  QAT    +A +        
Sbjct: 674  PQKVSAQRSLAPLQPSQQVVGHQTPNLRTPYPMSQSQGLTRQATLDRWEALKKGSSQGVT 733

Query: 1053 --------LNERQLSSQQMANLMMPRAMGQPPAMSQPSYSMNPNGRRMPSVVGDQWSNVG 1208
                     + R +++QQ   ++ P    Q P  + P    N +G R P +  DQ  + G
Sbjct: 734  RTTGLAGGQHTRVVATQQTTQVVRP---VQTPRAASPVLPGNSDGFRTP-LAQDQRGSTG 789

Query: 1209 VASPPLMTTDAYD-PAQQTWRPAGRMRGALSGQAYADAYNRFMSQHNQQAAQAARPISNV 1385
              +P   T  + D      WRP GRMRG+LSG+AY++A N+++ Q  QQ AQ+ RP    
Sbjct: 790  GTTPVTRTDSSGDSQLDPNWRPTGRMRGSLSGRAYSEALNQYILQPTQQ-AQSPRPSVPP 848

Query: 1386 HLDN------VNVPAESFMPPNYPSARPASLP 1463
            +L         N  A S  P N+PS  PA+ P
Sbjct: 849  NLSPQLQVFLANRGAHSTQPVNHPSTTPANAP 880


>ref|XP_022867113.1| E4 SUMO-protein ligase PIAL2-like isoform X3 [Olea europaea var.
            sylvestris]
 ref|XP_022867114.1| E4 SUMO-protein ligase PIAL2-like isoform X4 [Olea europaea var.
            sylvestris]
          Length = 507

 Score =  317 bits (811), Expect = 3e-99
 Identities = 216/533 (40%), Positives = 276/533 (51%), Gaps = 104/533 (19%)
 Frame = +3

Query: 177  LVKVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTGD---------GESPQP 329
            + KVLKEV  NV ++IISSDGSWNAV++++   +KPEDK S+ G          G    P
Sbjct: 1    MAKVLKEVGPNVKEVIISSDGSWNAVIETEDTTKKPEDKTSNVGHDAPSEIESTGLPGAP 60

Query: 330  VDLLDLTQTNDGMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQRSAHVE 509
            V++LDLT+ +DGMD V T E+ED KLS +  Q+ SMTQT SV P +ANT +VNQ    ++
Sbjct: 61   VEILDLTEIDDGMDAVATRESEDTKLSPTACQSLSMTQTPSVNPQMANTCDVNQNIGQID 120

Query: 510  DDFWSGIFLSSLGQGPSNEQIGGVFVPTSNYIASPPVLTDSFTTNGQVEGFNGNNLFTAS 689
            +DFWSGI++S+ G G            TSN  ++ P+   +  ++ +++ F+GN   + S
Sbjct: 121  EDFWSGIYMSTFGTG------------TSNVRSNLPI---AGVSSRELDAFHGNVPLSTS 165

Query: 690  VPQREPSLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQALPAQTSTSILQQR 869
            VP+ E +LPN LQLQQ+QFGNSN + EYGR PS+  +VTRTP A+QALPAQ   S+LQQR
Sbjct: 166  VPESETALPNILQLQQYQFGNSNMSNEYGRLPSISSHVTRTPIAIQALPAQAPASVLQQR 225

Query: 870  SRNTARR---------------------------ANLHQVSQMSSSPLHQYSGVQNPGQE 968
            SRN+                              ++  QVSQM SS L QYSG+Q P   
Sbjct: 226  SRNSTNTFMQNNPSAAAQASPSARLVPDGFSMLDSSSSQVSQMPSSSLQQYSGIQAP--- 282

Query: 969  NRSFPTVRPPHQNVVLQATNQVPDAQRVLNERQLSSQ-QMANLMMPRAM----GQPPAMS 1133
                               + VP+A RV NERQ +SQ QMANL    AM    GQ  +  
Sbjct: 283  -------------------HLVPNAYRVSNERQSTSQLQMANLAASHAMNQSFGQVQSSI 323

Query: 1134 QPS----------------------------------------------------YSMNP 1157
             PS                                                    YS+NP
Sbjct: 324  LPSANFLSPQMHGVGSQSGVDRSTGVIGSQQSRLMVPNQRNGQMARPVETSRAHTYSLNP 383

Query: 1158 NGRRMPSVVGDQWSNVGVASPPLMTTDAYDPAQQTWRPAGRMRGALSGQAYADAYNRFMS 1337
            + RRMPSV   Q  N G  S  +  TDAYD A Q WRP  RMRGALSGQAY+DA N+F+ 
Sbjct: 384  DARRMPSV-SHQIENTGGTSVTVPRTDAYDQADQNWRPTARMRGALSGQAYSDALNQFIL 442

Query: 1338 QHNQQAAQAARPISNVHL--DNVNVP---------AESFMPPNYPSARPASLP 1463
            +   Q  QAARPISN      NV  P         A+S   PN+PS   AS P
Sbjct: 443  RPTSQ-PQAARPISNAGSLPGNVRAPLQPHMADRVAQSPQLPNHPSIGQASRP 494


>ref|XP_010324636.1| PREDICTED: E4 SUMO-protein ligase PIAL1 isoform X3 [Solanum
            lycopersicum]
          Length = 715

 Score =  322 bits (826), Expect = 5e-99
 Identities = 223/560 (39%), Positives = 287/560 (51%), Gaps = 75/560 (13%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCPISF+RIKTPVKG  CKH+QCFDFDNY+DINSRRPSWRCPHCNQHVCFTDI IDQ + 
Sbjct: 150  NCPISFKRIKTPVKGRSCKHLQCFDFDNYIDINSRRPSWRCPHCNQHVCFTDIHIDQDMF 209

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDT--------GDGESPQPVDL 338
            KVLKEV  +V+D++ISSDGSW A+M+SD   +KP DK  +          DG S  P D+
Sbjct: 210  KVLKEVSEDVTDVMISSDGSWKAIMESDDHSEKPRDKTPEIAQDSPRRGSDGPSNAPGDV 269

Query: 339  LDLTQTNDGMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQRSA-HVEDD 515
            LDLT  +D M+     ETED   S++   N  M   +     ++N + +NQ  A  + DD
Sbjct: 270  LDLTDIDDDMN---PAETED---SKNFPTNIQMQSNVQKTTAVSNPNEINQTGAPDMTDD 323

Query: 516  FWSGIFLSSLGQGP----SNEQIGGVFVPTSNYIASPPVLTDSFTTNGQVEGFNGNNLFT 683
            FWS I+LSS G G     SN Q G    P    +   PV TD+ +     E   GN    
Sbjct: 324  FWSRIYLSSCGIGTSSSWSNMQTGSASEPARTDLVQLPVFTDAISPALNTE---GNTFIP 380

Query: 684  ASVPQREPSLPN--TLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQALPAQTSTSI 857
             S+ +   S  N   LQLQQFQFGNS  + EYGRFP+  R   R+P AVQALPAQ +T +
Sbjct: 381  TSILESGLSSSNLLQLQLQQFQFGNSALSNEYGRFPTAARPANRSPVAVQALPAQMNTPV 440

Query: 858  LQQRSRNTAR------------------------RANLHQVS---------QMSSSPLHQ 938
             QQR ++                           R+ L + S         +M+SS L Q
Sbjct: 441  PQQRQQSAMNPLLHAGPSAAAQDLPIASLSGSNLRSELERHSFSDLDLVQTRMTSSALPQ 500

Query: 939  YSGVQN--PGQEN--RSFPTVRPPHQNVVLQATNQVPDAQRVLNERQLSSQQMANLMMPR 1106
               + +  P Q +  R  P++R P+     Q  +Q     R    +Q SSQ   N  +P 
Sbjct: 501  KRSLPHVQPSQHSVGRQSPSMRTPYPMNQSQGPSQSATWDRWEALKQGSSQAGVNRALPG 560

Query: 1107 AMGQ------------PPAMSQPSYSMNPNG--RRMPSVVGDQ--WSNVGVASPPLMTTD 1238
                             P  S  + S  P    R    +  DQ   S+ G  +P   T  
Sbjct: 561  GQHARVVTTQQSTQVVRPVFSPRTVSPLPGSADRFRTPLPPDQRGSSSTGGTTPVTRTDS 620

Query: 1239 AYDP-AQQTWRPAGRMRGALSGQAYADAYNRFMSQHNQQAAQAARPISNVHLDN------ 1397
            + DP     WRP GRMRG+LSG+AY++A  +F+ +  QQ AQAARP    +L        
Sbjct: 621  SVDPQLDPNWRPTGRMRGSLSGRAYSEALQQFILKPTQQ-AQAARPSIPPNLSPQLQVLL 679

Query: 1398 VNVPAESFMPPNYPSARPAS 1457
             N  A S  P N+PS  PA+
Sbjct: 680  ANRGAHSTQPVNFPSTAPAN 699


>ref|XP_009783337.1| PREDICTED: uncharacterized protein LOC104231952 [Nicotiana
            sylvestris]
 ref|XP_016457244.1| PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X1 [Nicotiana
            tabacum]
          Length = 882

 Score =  326 bits (835), Expect = 9e-99
 Identities = 227/560 (40%), Positives = 296/560 (52%), Gaps = 73/560 (13%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCPISF+RIKTPVKGH CKH+QCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQ + 
Sbjct: 320  NCPISFKRIKTPVKGHSCKHLQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQDMD 379

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISD--------TGDGESPQPVDL 338
            KVL EV  +V+D++ISSDGSW A+M+SD   +KP DKI D          DG S  P D+
Sbjct: 380  KVLIEVGEDVTDVMISSDGSWKAIMESDDHTEKPRDKIPDFTQDSPQRGSDGFSNTPADV 439

Query: 339  LDLTQTNDGMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQR-SAHVEDD 515
            +DLT  +D ++ + T ETED K   SI    S  Q  +V   + N   +N    + + DD
Sbjct: 440  MDLTDIDDEINPMATCETEDSKNFPSIPNIHSNVQNTTV---VNNPSEINHTGGSDMADD 496

Query: 516  FWSGIFLSSLGQGPSNE----QIGGVFVPTSNYIASPPVLTDSFTTNGQVEGFNGNNLFT 683
            FWS +++SS G G S      Q G    P+   +  PPVLTD+ + +   E   GN L  
Sbjct: 497  FWSRMYMSSCGIGTSGSLSSLQNGSASEPSRTNLLQPPVLTDAISPSFNPE---GNALIP 553

Query: 684  ASVPQREPSLPNTLQLQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQALPA-----QTS 848
             S+ +   S  + +QLQ FQFGNS  + EYGRFPS   +  RTP AVQALPA     QT 
Sbjct: 554  TSLLESGLSSSDMMQLQHFQFGNSAVSNEYGRFPSAAMHANRTPAAVQALPALPAQMQTP 613

Query: 849  TSILQQRSRN-----------------TARRANL------HQVS---------QMSSSPL 932
                QQ + N                 +   +N+      HQ S         +M+SS L
Sbjct: 614  VPQRQQSAMNPLLHTGPSVATQGLPTVSLNGSNVRCDLERHQCSIPDLDLLQARMTSSAL 673

Query: 933  HQ--YSGVQNPGQE--NRSFPTVRPPH-----QNVVLQATNQVPDA------QRVLNERQ 1067
             Q  +     P Q+   R  P +R P+     +++  QAT    +A      Q V     
Sbjct: 674  PQKRFVAPLQPSQQVVGRQTPNLRTPYPMSQSEDLTRQATLDRWEALKKGSSQGVTRATG 733

Query: 1068 LSSQQMANLMMPRAMGQPPAMSQPSYSM-NPNGRRMPSVVGDQWSNVGVASPPLMTTDAY 1244
            L+  Q   ++   A  Q   + +P+ S  N +G R P +  D   N G  +P   T  + 
Sbjct: 734  LAGGQHTRVV---ATQQTTQVVRPAQSPGNSDGFRTP-LAWDHRGNTGGTTPVTRTDSSG 789

Query: 1245 D-PAQQTWRPAGRMRGALSGQAYADAYNRFMSQHNQQAAQAARPISNVHLDN------VN 1403
            D      WRP GRMRG+LSG+AY++A N+++ Q  QQ AQA RP    +L         N
Sbjct: 790  DVQLDPNWRPTGRMRGSLSGRAYSEALNQYILQPTQQ-AQAPRPSVPPNLSPQLQVFLAN 848

Query: 1404 VPAESFMPPNYPSARPASLP 1463
              A S  P N+PS  PA+ P
Sbjct: 849  RGAHSTQPVNHPSTTPANAP 868


>ref|XP_006352265.1| PREDICTED: uncharacterized protein LOC102591021 isoform X2 [Solanum
            tuberosum]
          Length = 878

 Score =  323 bits (828), Expect = 9e-98
 Identities = 222/563 (39%), Positives = 288/563 (51%), Gaps = 78/563 (13%)
 Frame = +3

Query: 3    NCPISFRRIKTPVKGHLCKHIQCFDFDNYVDINSRRPSWRCPHCNQHVCFTDIRIDQKLV 182
            NCPISF+RIKTPVKGH CKH+QCFDFDNY+DINSRRPSWRCPHCNQHVCFTDI IDQ + 
Sbjct: 314  NCPISFKRIKTPVKGHSCKHLQCFDFDNYIDINSRRPSWRCPHCNQHVCFTDIHIDQDMF 373

Query: 183  KVLKEVEANVSDIIISSDGSWNAVMDSDTAIQKPEDKISDTG--------DGESPQPVDL 338
            KVLKEV  +V+D++ISSDGSW A+M+SD   +KP DK  +          DG S  P D+
Sbjct: 374  KVLKEVSEDVTDVMISSDGSWKAIMESDDYSEKPRDKTPEIAQDSPRRGSDGPSNAPGDV 433

Query: 339  LDLTQTNDGMDFVTTNETEDRKLSRSISQNQSMTQTISVEPHIANTDNVNQRSA-HVEDD 515
            LDLT  +D M+     ETED   S++   N  M   +     + N   +NQ  A  + DD
Sbjct: 434  LDLTDIDDDMN---PAETED---SKNFPTNIQMQSNVQKTTAVNNPSEINQTGAPDMTDD 487

Query: 516  FWSGIFLSSLGQGPSNE----QIGGVFVPTSNYIASPPVLTDSFTTNGQVEGFNGNNLFT 683
            FWS I+LSS G G SN     Q G    P    +   PV TD+ +     EG   N    
Sbjct: 488  FWSRIYLSSCGIGTSNSWSSMQTGSASEPARTDLVQLPVFTDAISPAFNTEG---NAFIP 544

Query: 684  ASVPQREPSLPNTLQ--LQQFQFGNSNYTAEYGRFPSLPRNVTRTPTAVQALPAQTSTSI 857
             SV +   S  N LQ  LQQFQFGNS  + EYGRFP+  R   RTP AVQALPAQ +T +
Sbjct: 545  TSVLESGLSSSNLLQMQLQQFQFGNSALSNEYGRFPTGARPANRTPVAVQALPAQMNT-L 603

Query: 858  LQQRSRNTARR---------------ANLHQVSQMSSSPLHQYSGVQ--------NPGQE 968
             QQR ++T                  A+L   +  S    H +S +         +   +
Sbjct: 604  PQQRQQSTMNPLFHAGPSAATQDLPIASLDGSNLRSELERHSFSDLDLVQARTTSSALPQ 663

Query: 969  NRSFPTVRPPHQNVVLQ----------ATNQVPDAQRVLNERQLSSQQMANLMMPRAMG- 1115
             RS P V+P   +V  Q          + +Q P      +  +   Q  + + + RA+  
Sbjct: 664  KRSLPHVQPSQHSVGRQTPSMRTPYSMSQSQGPTQSATWDRWEALKQGSSQVGVNRALAG 723

Query: 1116 ----------QPPAMSQPSYSMNPNGRRMPSVVG-----------DQWSNVGVASPPLMT 1232
                      Q   + +P YS     R +P + G           DQ  + G  +P   T
Sbjct: 724  GQHTRVVTTQQTTQVVRPVYSP----RTVPPLPGSADRFRTPLAPDQRVSTGGMTPVTRT 779

Query: 1233 TDAYDPA-QQTWRPAGRMRGALSGQAYADAYNRFMSQHNQQA-AQAARPISNVHLDN--- 1397
              + DP     WRP GRMRG+LSG+AY++A  +F+ +  QQA AQAARP    +L     
Sbjct: 780  DSSVDPQLDPNWRPTGRMRGSLSGRAYSEALQQFILKPTQQAQAQAARPSIPPNLSPQLQ 839

Query: 1398 ---VNVPAESFMPPNYPSARPAS 1457
                N  A +  P N+PS  PA+
Sbjct: 840  VLLANRGAHNTQPVNFPSTAPAN 862