BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia32_contig00008773
         (1968 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011099009.1| threonylcarbamoyladenosine tRNA methylthiotr...  1008   0.0  
gb|PIN00797.1| putative Fe-S oxidoreductase [Handroanthus impeti...  1004   0.0  
ref|XP_012854837.1| PREDICTED: threonylcarbamoyladenosine tRNA m...  1001   0.0  
ref|XP_022895041.1| threonylcarbamoyladenosine tRNA methylthiotr...   928   0.0  
gb|KZV40882.1| threonylcarbamoyladenosine tRNA methylthiotransfe...   916   0.0  
ref|XP_023875593.1| threonylcarbamoyladenosine tRNA methylthiotr...   873   0.0  
gb|EOY17308.1| Methylthiotransferase [Theobroma cacao]                865   0.0  
gb|POE82136.1| threonylcarbamoyladenosine trna methylthiotransfe...   864   0.0  
ref|XP_007020083.2| PREDICTED: threonylcarbamoyladenosine tRNA m...   864   0.0  
ref|XP_019231974.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   864   0.0  
ref|XP_016438122.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   863   0.0  
ref|XP_011001084.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   862   0.0  
ref|XP_021288193.1| threonylcarbamoyladenosine tRNA methylthiotr...   862   0.0  
ref|XP_009587336.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   861   0.0  
ref|XP_024174573.1| threonylcarbamoyladenosine tRNA methylthiotr...   861   0.0  
ref|XP_006350322.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   860   0.0  
gb|PNT07771.1| hypothetical protein POPTR_013G107800v3 [Populus ...   860   0.0  
gb|PRQ20424.1| putative tRNA (N(6)-L-threonylcarbamoyladenosine(...   861   0.0  
ref|XP_004250429.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   858   0.0  
ref|XP_015058303.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   857   0.0  

>ref|XP_011099009.1| threonylcarbamoyladenosine tRNA methylthiotransferase [Sesamum
            indicum]
          Length = 602

 Score = 1008 bits (2606), Expect = 0.0
 Identities = 506/606 (83%), Positives = 546/606 (90%)
 Frame = -3

Query: 1963 MEDIEDXXXXXXXXXXXGFRLXXXXXXXXXXXXXAFQDSLKSYNTAKIPGTQTIYVKTFG 1784
            MEDIED           G RL               QDSLKSY  AKIPGTQTIY++TFG
Sbjct: 1    MEDIEDLLVGGGGGGPPGVRLVQPKHKKKNNN---IQDSLKSYTAAKIPGTQTIYMRTFG 57

Query: 1783 CSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQSAMETLIAKCRNAKKP 1604
            CSHNQSDSEYMAGQLSAFGYNL+E+QDEADLWLINTCTVKSPSQSAM+TLI+KCR+AKKP
Sbjct: 58   CSHNQSDSEYMAGQLSAFGYNLTESQDEADLWLINTCTVKSPSQSAMDTLISKCRSAKKP 117

Query: 1603 LVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLTRKTLPALDLPKV 1424
            LVVAGCVPQGSRD+KELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLTRKTLPALDLPKV
Sbjct: 118  LVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLTRKTLPALDLPKV 177

Query: 1423 RKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVRTVIADGVKEIWLSSED 1244
            R+NKFVEILPINVGCLGACTYCKTKHARGHLGSY VDSLVGRVRTVIADGVKEIWLSSED
Sbjct: 178  RRNKFVEILPINVGCLGACTYCKTKHARGHLGSYAVDSLVGRVRTVIADGVKEIWLSSED 237

Query: 1243 TGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTNPPYILEHLKQIAEVLRHPCVYSF 1064
            TGAYGRDIGVNLPILL+SLVAELPSDGSTMLRIGMTNPPYILEHLK+IAEVLRHP VYSF
Sbjct: 238  TGAYGRDIGVNLPILLNSLVAELPSDGSTMLRIGMTNPPYILEHLKEIAEVLRHPSVYSF 297

Query: 1063 LHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVPGMQIATDIICGFPGETDEDFEQT 884
            LHVPVQSGSDTILTAMNREYTV EFRTV DTLIELVPGMQIATDIICGFPGETDEDFEQT
Sbjct: 298  LHVPVQSGSDTILTAMNREYTVGEFRTVVDTLIELVPGMQIATDIICGFPGETDEDFEQT 357

Query: 883  VNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELTTIFESFTPYVGMEGKI 704
            VNLIKEY+F QVHISQFYPRPGTPAARMKKVPSNVVKKRSRELTT+FESF PYVGMEGKI
Sbjct: 358  VNLIKEYQFPQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELTTVFESFNPYVGMEGKI 417

Query: 703  ERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTSAIVKITSVGRWSVFGEVIEILSE 524
            ERIWIT++ATDG+HLVGHTKGYIQVLVVG E++LG SA+VKITSVGRWSVFGEVIEIL+E
Sbjct: 418  ERIWITDIATDGNHLVGHTKGYIQVLVVGPETMLGISAMVKITSVGRWSVFGEVIEILTE 477

Query: 523  NANSRKNHGSVEENSCSNLKETCCSKEQETCACGLTSGCGETEEKTVSVSKDDQKSGNYR 344
            N  S +N   V++ SCSNL ETCCSK+ ETCACGLTSGC +TE+   SVSKDD+K  +YR
Sbjct: 478  NVISHEN--CVDKISCSNLNETCCSKDSETCACGLTSGCEQTEKNNFSVSKDDRKPVDYR 535

Query: 343  SQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQETSSQMYEWALLDKALLVGIIMSLSTII 164
            SQNL+GW LRKRK++SHR  ENEMVS+++EKQ TS++++EWAL+DKALLVGI MSL TII
Sbjct: 536  SQNLIGWLLRKRKDNSHRKVENEMVSEMEEKQRTSAKLHEWALVDKALLVGIFMSLLTII 595

Query: 163  AMLCIW 146
            A+  IW
Sbjct: 596  AVFFIW 601


>gb|PIN00797.1| putative Fe-S oxidoreductase [Handroanthus impetiginosus]
          Length = 627

 Score = 1004 bits (2597), Expect = 0.0
 Identities = 508/627 (81%), Positives = 547/627 (87%), Gaps = 20/627 (3%)
 Frame = -3

Query: 1963 MEDIEDXXXXXXXXXXXGFRLXXXXXXXXXXXXXA----FQDSLKSYNTAKIPGTQTIYV 1796
            MEDIED           GFRL                   +DSLKSY+TAKIPGTQTIY+
Sbjct: 1    MEDIEDLLVGGGSGAPPGFRLPVGAAVGVKPKHKKKNNLLRDSLKSYDTAKIPGTQTIYL 60

Query: 1795 KTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQSAMETLIAKCRN 1616
            KTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQSAM+TLIAK RN
Sbjct: 61   KTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQSAMDTLIAKGRN 120

Query: 1615 AKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLTRKTLPALD 1436
            A KPLVVAGCVPQGSR++KELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLTRKTLPALD
Sbjct: 121  ANKPLVVAGCVPQGSRNLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLTRKTLPALD 180

Query: 1435 LPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVRTVIADGVKEIWL 1256
            LPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVR+VIADGVKEIWL
Sbjct: 181  LPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVRSVIADGVKEIWL 240

Query: 1255 SSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTNPPYILEHLKQIAEVLRHPC 1076
            SSEDTGAYGRDIGVNLPILL+SLVAELPSDGSTMLRIGMTNPPYILEHLKQIAEVLRHPC
Sbjct: 241  SSEDTGAYGRDIGVNLPILLNSLVAELPSDGSTMLRIGMTNPPYILEHLKQIAEVLRHPC 300

Query: 1075 VYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVPGMQIATDIICGFPGETDED 896
            VYSFLHVPVQSGSDTILTAMNREYTVSEF+ V DTLIELVPGMQIATDIICGFPGETDED
Sbjct: 301  VYSFLHVPVQSGSDTILTAMNREYTVSEFKKVVDTLIELVPGMQIATDIICGFPGETDED 360

Query: 895  FEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELTTIFESFTPYVGM 716
            FEQTVNLIKEYKF QVHISQFYPRPGTPAARMKKVPSN+VKKRSRELTT+FESF PYVGM
Sbjct: 361  FEQTVNLIKEYKFPQVHISQFYPRPGTPAARMKKVPSNIVKKRSRELTTVFESFMPYVGM 420

Query: 715  EGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTSAIVKITSVGRWSVFGEVIE 536
            EGK+ERIWIT++ATDGSHLVGHTKGYIQVLVVG ES+LG SA+VKITS GRWSVFGEVI+
Sbjct: 421  EGKVERIWITDIATDGSHLVGHTKGYIQVLVVGPESMLGASAMVKITSFGRWSVFGEVIK 480

Query: 535  ILSENANSRKNHGSVEENSCSNLKETCCSKEQET----------------CACGLTSGCG 404
            IL+ENANS KN   VE+ SCSN  ETCCSKE ET                CAC LTS CG
Sbjct: 481  ILTENANSMKNQVRVEKLSCSNSNETCCSKESETCCSKESETCCSKESGMCACELTSSCG 540

Query: 403  ETEEKTVSVSKDDQKSGNYRSQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQETSSQMYE 224
            +TE+KTVSVS D+QK G+Y+SQN++GW LRKRKN +H+  ENE+V + ++K+ TSS+  +
Sbjct: 541  QTEDKTVSVSNDEQKPGDYKSQNILGWLLRKRKNYAHKKTENEIVLESEKKRGTSSRADK 600

Query: 223  WALLDKALLVGIIMSLSTIIAMLCIWI 143
            WAL+DKALLVGI+MSL  IIA+ C W+
Sbjct: 601  WALVDKALLVGIVMSLLAIIAIFCFWV 627


>ref|XP_012854837.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase
            [Erythranthe guttata]
 gb|EYU22794.1| hypothetical protein MIMGU_mgv1a003138mg [Erythranthe guttata]
          Length = 605

 Score = 1001 bits (2587), Expect = 0.0
 Identities = 500/608 (82%), Positives = 540/608 (88%), Gaps = 1/608 (0%)
 Frame = -3

Query: 1963 MEDIEDXXXXXXXXXXXGFRLXXXXXXXXXXXXXAFQDSLKSYNTAKIPGTQTIYVKTFG 1784
            MEDIED           GFR+               +D LK Y+T+KIPGTQTIY+KTFG
Sbjct: 1    MEDIEDLLVGGGGGAPPGFRIPVAAAVGIKPKKSVSRDYLKDYSTSKIPGTQTIYIKTFG 60

Query: 1783 CSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQSAMETLIAKCRNAKKP 1604
            CSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQSAM+TLI KCRNAKKP
Sbjct: 61   CSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQSAMDTLITKCRNAKKP 120

Query: 1603 LVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLTRKTLPALDLPKV 1424
            LVVAGCVPQGSRD+KEL+GVSIVGVQQIDRVVEVVEETLKGHEVRLLTRKTLPALDLPKV
Sbjct: 121  LVVAGCVPQGSRDLKELDGVSIVGVQQIDRVVEVVEETLKGHEVRLLTRKTLPALDLPKV 180

Query: 1423 RKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVRTVIADGVKEIWLSSED 1244
            RKN FVEILPINVGCLGACTYCKTKHARGHLGSYTVD LVGRVR+VI DGVKEIWLSSED
Sbjct: 181  RKNMFVEILPINVGCLGACTYCKTKHARGHLGSYTVDGLVGRVRSVIDDGVKEIWLSSED 240

Query: 1243 TGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTNPPYILEHLKQIAEVLRHPCVYSF 1064
            TGAYGRDIGVNLPILL+SLVAELPSDGSTMLRIGMTNPPYILEHLK+IAEVLRHPCVYSF
Sbjct: 241  TGAYGRDIGVNLPILLNSLVAELPSDGSTMLRIGMTNPPYILEHLKEIAEVLRHPCVYSF 300

Query: 1063 LHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVPGMQIATDIICGFPGETDEDFEQT 884
            LHVPVQSGSD +LTAMNREYTV EFRTV DTL+ELVPGMQIATDIICGFPGETDEDFE T
Sbjct: 301  LHVPVQSGSDRVLTAMNREYTVGEFRTVVDTLMELVPGMQIATDIICGFPGETDEDFEAT 360

Query: 883  VNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELTTIFESFTPYVGMEGKI 704
            VNLIK+YK  QVHISQFYPRPGTPAARMKKVPSN+VK+RSRELTT+FESFTPYVGMEGKI
Sbjct: 361  VNLIKDYKLPQVHISQFYPRPGTPAARMKKVPSNIVKRRSRELTTVFESFTPYVGMEGKI 420

Query: 703  ERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTSAIVKITSVGRWSVFGEVIEILSE 524
            ERIWIT++ATDGSHLVGHTKGYIQVLVVG ES+LG+SA VKITSVGRWSVFGEVI+I S+
Sbjct: 421  ERIWITDIATDGSHLVGHTKGYIQVLVVGPESMLGSSATVKITSVGRWSVFGEVIQIHSQ 480

Query: 523  NANSRKNHGSVEENSCSNLKETCCSKEQETCACGLTSGCGETEEKT-VSVSKDDQKSGNY 347
            N   RKN   VE+NSCSN KETCCS+E ETCACGLTSGCG+TEE T VSV KDDQK+G  
Sbjct: 481  NTKPRKNSARVEKNSCSNSKETCCSEESETCACGLTSGCGQTEEATDVSVLKDDQKTGEI 540

Query: 346  RSQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQETSSQMYEWALLDKALLVGIIMSLSTI 167
            RSQNL+G+ LRKRKNSSHR  EN++V ++D  + TS+   +WAL+DKALLVGI+MSL TI
Sbjct: 541  RSQNLIGYLLRKRKNSSHRKIENQVVKEMDGNEGTSN---DWALVDKALLVGIVMSLLTI 597

Query: 166  IAMLCIWI 143
            IA+   W+
Sbjct: 598  IAIFRFWL 605


>ref|XP_022895041.1| threonylcarbamoyladenosine tRNA methylthiotransferase-like [Olea
            europaea var. sylvestris]
          Length = 627

 Score =  928 bits (2398), Expect = 0.0
 Identities = 461/565 (81%), Positives = 503/565 (89%), Gaps = 1/565 (0%)
 Frame = -3

Query: 1852 DSLKSYNTAKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTC 1673
            DS+ SY+T KIPGT+TIY+KTFGCSHNQSDSEYMAGQLSAFGYNLS+N D+ADLWLINTC
Sbjct: 51   DSVSSYDTTKIPGTETIYMKTFGCSHNQSDSEYMAGQLSAFGYNLSDNPDDADLWLINTC 110

Query: 1672 TVKSPSQSAMETLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEE 1493
            TVKSPSQSAMETLIAKCRN  KPLVVAGCVPQGSRD+KELEGVSIVGVQQIDRVVEVVEE
Sbjct: 111  TVKSPSQSAMETLIAKCRNGNKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEE 170

Query: 1492 TLKGHEVRLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVD 1313
            TLKGHEVRLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVD
Sbjct: 171  TLKGHEVRLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVD 230

Query: 1312 SLVGRVRTVIADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTN 1133
            SLVGR+R+VIADGV+EIWLSSEDTGAYGRD+GVNL ILL++LV ELPSDGSTMLRIGMTN
Sbjct: 231  SLVGRIRSVIADGVREIWLSSEDTGAYGRDLGVNLTILLNALVVELPSDGSTMLRIGMTN 290

Query: 1132 PPYILEHLKQIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVP 953
            PPY+LEHLK+IAEVLRHPCVYSFLHVPVQSGSDTIL+AMNREYTV EF+TV DTL E+VP
Sbjct: 291  PPYMLEHLKEIAEVLRHPCVYSFLHVPVQSGSDTILSAMNREYTVGEFKTVVDTLTEMVP 350

Query: 952  GMQIATDIICGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVK 773
            GMQIATDIICGFPGETDEDF+QTVNLIKEYKF+QVHISQFYPRPGTPAARMKKVPS VVK
Sbjct: 351  GMQIATDIICGFPGETDEDFQQTVNLIKEYKFSQVHISQFYPRPGTPAARMKKVPSTVVK 410

Query: 772  KRSRELTTIFESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTS 593
            +RSRELT++FESFTPYVGMEGK+ERIWITE+ATDG+HLVGHTKGYIQVLV+G ES+LGTS
Sbjct: 411  RRSRELTSVFESFTPYVGMEGKVERIWITEIATDGTHLVGHTKGYIQVLVLGQESMLGTS 470

Query: 592  AIVKITSVGRWSVFGEVIEILSENANSRKNHGSVEENSCSNLKETC-CSKEQETCACGLT 416
            AIVKI SVGRWSVFGEVIEIL+  + S ++  ++  +  SNL  TC CSKE E CAC L 
Sbjct: 471  AIVKIMSVGRWSVFGEVIEILNRGSASSESRANI-NSPRSNLDGTCACSKEPEPCACELK 529

Query: 415  SGCGETEEKTVSVSKDDQKSGNYRSQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQETSS 236
            S CG TEEK VSV ++      Y SQNL+GW LRKRKNS H+  ENE V + DE   TS 
Sbjct: 530  SSCGHTEEKIVSVLRNVPTPSEYSSQNLIGWLLRKRKNSFHKRMENEAVLEYDENPGTSH 589

Query: 235  QMYEWALLDKALLVGIIMSLSTIIA 161
                W   DKAL  GI++SL TIIA
Sbjct: 590  HGCNWGPADKALFGGILVSLLTIIA 614


>gb|KZV40882.1| threonylcarbamoyladenosine tRNA methylthiotransferase-like
            [Dorcoceras hygrometricum]
          Length = 615

 Score =  916 bits (2368), Expect = 0.0
 Identities = 462/568 (81%), Positives = 515/568 (90%), Gaps = 2/568 (0%)
 Frame = -3

Query: 1840 SYNTAKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKS 1661
            S N++KIPGTQTIY+KTFGCSHNQSDSEYMAGQLSAFGYNL EN+DEADLWL+NTCTVKS
Sbjct: 49   SRNSSKIPGTQTIYMKTFGCSHNQSDSEYMAGQLSAFGYNLCENEDEADLWLLNTCTVKS 108

Query: 1660 PSQSAMETLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEETLKG 1481
            PSQSAM+TLIAKC++AKKPLVVAGCVPQGSRD+K+L+GVSIVGVQQIDRVVEVVEETLKG
Sbjct: 109  PSQSAMQTLIAKCKSAKKPLVVAGCVPQGSRDLKDLDGVSIVGVQQIDRVVEVVEETLKG 168

Query: 1480 HEVRLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVG 1301
            HEVRLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSL+G
Sbjct: 169  HEVRLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLMG 228

Query: 1300 RVRTVIADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTNPPYI 1121
            RVRTV+ADGV+EIWLSSEDTGAYGRDIGV+LPILL+S V+ELP DGSTMLRIGMTNPPYI
Sbjct: 229  RVRTVVADGVREIWLSSEDTGAYGRDIGVSLPILLNSFVSELPPDGSTMLRIGMTNPPYI 288

Query: 1120 LEHLKQIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVPGMQI 941
            LEHLK+IAEVLRHPCVYSFLHVPVQSGSD+IL AMNREYTVSEFRTV DTLIELVPGMQI
Sbjct: 289  LEHLKEIAEVLRHPCVYSFLHVPVQSGSDSILRAMNREYTVSEFRTVVDTLIELVPGMQI 348

Query: 940  ATDIICGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVKKRSR 761
            ATD+ICGFPGETDEDFEQT+NLIKEYKF QVHISQFYPRPGTPAARMKKVPSNVVKKRSR
Sbjct: 349  ATDVICGFPGETDEDFEQTINLIKEYKFPQVHISQFYPRPGTPAARMKKVPSNVVKKRSR 408

Query: 760  ELTTIFESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTSAIVK 581
            ELT IFESFTPYVGMEGKIERIWIT+VATDG+HLVGHTKGYIQVLVVG  S+LG+SAIVK
Sbjct: 409  ELTAIFESFTPYVGMEGKIERIWITDVATDGTHLVGHTKGYIQVLVVGPGSMLGSSAIVK 468

Query: 580  ITSVGRWSVFGEVIEILSENANSRKNHGSVEENSCSNLKE-TCCSKEQE-TCACGLTSGC 407
            ITSVGRWSVFG+VIEIL+  A+SR+N G+  ++ CSN  E TCCSK+ E TCAC  TS  
Sbjct: 469  ITSVGRWSVFGDVIEILTGQAHSRENLGN-RDSKCSNENETTCCSKDSETTCACEWTSRF 527

Query: 406  GETEEKTVSVSKDDQKSGNYRSQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQETSSQMY 227
            G+ EE TVSVSK+++K G   S+NL GW LRKRK +  +  ENE+V + +EKQ    ++ 
Sbjct: 528  GQKEEGTVSVSKNERKPG---SENLFGWLLRKRKKNFQKETENEVVLEFEEKQ----RIN 580

Query: 226  EWALLDKALLVGIIMSLSTIIAMLCIWI 143
            EWA +D ALL  ++ SL T +A +C+++
Sbjct: 581  EWAFVDYALLGVMLTSLLTAVA-ICLYL 607


>ref|XP_023875593.1| threonylcarbamoyladenosine tRNA methylthiotransferase [Quercus suber]
 ref|XP_023875594.1| threonylcarbamoyladenosine tRNA methylthiotransferase [Quercus suber]
          Length = 625

 Score =  873 bits (2255), Expect = 0.0
 Identities = 438/571 (76%), Positives = 491/571 (85%), Gaps = 13/571 (2%)
 Frame = -3

Query: 1828 AKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQS 1649
            ++IPG QTIY+KTFGCSHNQSDSEYMAGQL+AFGY+LS++ +EADLWLINTCTVKSPSQS
Sbjct: 44   SQIPGIQTIYIKTFGCSHNQSDSEYMAGQLAAFGYSLSDSPEEADLWLINTCTVKSPSQS 103

Query: 1648 AMETLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEETLKGHEVR 1469
            AM+TLIAKC+NAKKPLVVAGCVPQGSRD+KELEGVSIVGVQQIDRVVEVVEETLKGHEVR
Sbjct: 104  AMDTLIAKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR 163

Query: 1468 LLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVRT 1289
            LL RKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYT+DSLVGRV T
Sbjct: 164  LLNRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTIDSLVGRVNT 223

Query: 1288 VIADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTNPPYILEHL 1109
            VIADGVKEIWLSSEDTGAYGRDIGVNLP LL++LVAELP D STMLRIGMTNPP+ILEHL
Sbjct: 224  VIADGVKEIWLSSEDTGAYGRDIGVNLPTLLNALVAELPPDASTMLRIGMTNPPFILEHL 283

Query: 1108 KQIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVPGMQIATDI 929
            K+IAEVLRHPCVYSFLHVPVQSGSD +L AMNREYTVSEFRTV DTL +LVPGMQIATDI
Sbjct: 284  KEIAEVLRHPCVYSFLHVPVQSGSDAVLNAMNREYTVSEFRTVVDTLTDLVPGMQIATDI 343

Query: 928  ICGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELTT 749
            ICGFPGETDEDF QTV+L+KEYKF QVHISQFYPRPGTPAARMKKVPSNVVKKRSRELT+
Sbjct: 344  ICGFPGETDEDFVQTVSLVKEYKFPQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELTS 403

Query: 748  IFESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTSAIVKITSV 569
            +FE+FTPY GMEG++ERIWITE+ATDG HLVGHTKGY+QVLV+  ES+LGTSAIVKITSV
Sbjct: 404  VFEAFTPYNGMEGRLERIWITEIATDGIHLVGHTKGYVQVLVIARESMLGTSAIVKITSV 463

Query: 568  GRWSVFGEVIEILS----ENANSRKNHGSVEENSCSNLKETC-CSKEQETCACGLTS--- 413
            GRWSVFGEVIE ++    E++   K     + + CSN  E+C C++E E C CG  S   
Sbjct: 464  GRWSVFGEVIETINHINDESSLRNKKPSQAKCSPCSNPVESCACTREPEPCPCGPESCGD 523

Query: 412  ----GCGETEEKTVSVSKDDQKSGNYRSQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQE 245
                 CG T  +  SVS++   + +  S+NL+GW LRKRKN  H+  E E+     +KQE
Sbjct: 524  CGPESCGPTTIEESSVSRNSMFAEDLNSRNLIGWLLRKRKNHVHKKVECEIALGSMKKQE 583

Query: 244  -TSSQMYEWALLDKALLVGIIMSLSTIIAML 155
                 M EW ++DKALLVGI +S  TI+A+L
Sbjct: 584  WAQGSMGEWGVVDKALLVGIFVSFLTIMALL 614


>gb|EOY17308.1| Methylthiotransferase [Theobroma cacao]
          Length = 623

 Score =  865 bits (2234), Expect = 0.0
 Identities = 431/570 (75%), Positives = 483/570 (84%), Gaps = 4/570 (0%)
 Frame = -3

Query: 1852 DSLKSYNTAKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTC 1673
            D L      KIPGTQTIY+KTFGCSHNQSDSEYMAGQLSAFGY +S+N +E DLWLINTC
Sbjct: 45   DPLTPLLPPKIPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYAISDNPEEGDLWLINTC 104

Query: 1672 TVKSPSQSAMETLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEE 1493
            TVKSPSQSAM TLI KC++AKKPLVVAGCVPQGSRD+KELEGVSIVGVQQIDRVVEVVEE
Sbjct: 105  TVKSPSQSAMNTLITKCKSAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEE 164

Query: 1492 TLKGHEVRLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVD 1313
            TLKGHEVRL+ RKTLP LDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYT+D
Sbjct: 165  TLKGHEVRLMNRKTLPGLDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTID 224

Query: 1312 SLVGRVRTVIADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTN 1133
            SLV RV+TVIADGVKEIWLSSEDTGAYGRDIGVNLPILL++LVAELP DG+TMLRIGMTN
Sbjct: 225  SLVERVKTVIADGVKEIWLSSEDTGAYGRDIGVNLPILLNALVAELPPDGNTMLRIGMTN 284

Query: 1132 PPYILEHLKQIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVP 953
            PP+ILEHLK+IA+VLRHPCVYSFLHVPVQSGSD ILTAMNREYTVSEFRTV DTL ELVP
Sbjct: 285  PPFILEHLKEIADVLRHPCVYSFLHVPVQSGSDAILTAMNREYTVSEFRTVVDTLTELVP 344

Query: 952  GMQIATDIICGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVK 773
            GMQIATDIICGFPGETDEDF QTVNLIKEYKF QVHISQFYPRPGTPAARMKKVPS +VK
Sbjct: 345  GMQIATDIICGFPGETDEDFAQTVNLIKEYKFPQVHISQFYPRPGTPAARMKKVPSTIVK 404

Query: 772  KRSRELTTIFESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTS 593
             RSRELT++FE+FTPY+GMEG++ERIWITEVA DG HLVGHTKGY+QVLV+G ES+LGTS
Sbjct: 405  NRSRELTSVFEAFTPYIGMEGRVERIWITEVAADGIHLVGHTKGYVQVLVIGPESMLGTS 464

Query: 592  AIVKITSVGRWSVFGEVIEILSENANSRKNHGSVEENSCS----NLKETCCSKEQETCAC 425
            AIVKITSVGRWSVFGEVIE L++            +  CS          CSKE E C+C
Sbjct: 465  AIVKITSVGRWSVFGEVIETLTQVNLKMAPEKVCNQEKCSTRFNQFDSGACSKEPEPCSC 524

Query: 424  GLTSGCGETEEKTVSVSKDDQKSGNYRSQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQE 245
            G  S  G+T  +  +VS +D    +  S+NL+GW LRKR+N  H+  EN +   L +KQ+
Sbjct: 525  GPNSCGGQTILEECTVSSNDSWMEDRSSKNLIGWLLRKRRNHVHKKVENGLALGLRKKQD 584

Query: 244  TSSQMYEWALLDKALLVGIIMSLSTIIAML 155
             +  +  W ++D+ALLVGI++S   I+A++
Sbjct: 585  WA--LGGWGIVDRALLVGILVSFFIIVAVI 612


>gb|POE82136.1| threonylcarbamoyladenosine trna methylthiotransferase [Quercus suber]
          Length = 619

 Score =  864 bits (2233), Expect = 0.0
 Identities = 433/562 (77%), Positives = 484/562 (86%), Gaps = 13/562 (2%)
 Frame = -3

Query: 1828 AKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQS 1649
            ++IPG QTIY+KTFGCSHNQSDSEYMAGQL+AFGY+LS++ +EADLWLINTCTVKSPSQS
Sbjct: 44   SQIPGIQTIYIKTFGCSHNQSDSEYMAGQLAAFGYSLSDSPEEADLWLINTCTVKSPSQS 103

Query: 1648 AMETLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEETLKGHEVR 1469
            AM+TLIAKC+NAKKPLVVAGCVPQGSRD+KELEGVSIVGVQQIDRVVEVVEETLKGHEVR
Sbjct: 104  AMDTLIAKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR 163

Query: 1468 LLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVRT 1289
            LL RKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYT+DSLVGRV T
Sbjct: 164  LLNRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTIDSLVGRVNT 223

Query: 1288 VIADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTNPPYILEHL 1109
            VIADGVKEIWLSSEDTGAYGRDIGVNLP LL++LVAELP D STMLRIGMTNPP+ILEHL
Sbjct: 224  VIADGVKEIWLSSEDTGAYGRDIGVNLPTLLNALVAELPPDASTMLRIGMTNPPFILEHL 283

Query: 1108 KQIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVPGMQIATDI 929
            K+IAEVLRHPCVYSFLHVPVQSGSD +L AMNREYTVSEFRTV DTL +LVPGMQIATDI
Sbjct: 284  KEIAEVLRHPCVYSFLHVPVQSGSDAVLNAMNREYTVSEFRTVVDTLTDLVPGMQIATDI 343

Query: 928  ICGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELTT 749
            ICGFPGETDEDF QTV+L+KEYKF QVHISQFYPRPGTPAARMKKVPSNVVKKRSRELT+
Sbjct: 344  ICGFPGETDEDFVQTVSLVKEYKFPQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELTS 403

Query: 748  IFESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTSAIVKITSV 569
            +FE+FTPY GMEG++ERIWITE+ATDG HLVGHTKGY+QVLV+  ES+LGTSAIVKITSV
Sbjct: 404  VFEAFTPYNGMEGRLERIWITEIATDGIHLVGHTKGYVQVLVIARESMLGTSAIVKITSV 463

Query: 568  GRWSVFGEVIEILS----ENANSRKNHGSVEENSCSNLKETC-CSKEQETCACGLTS--- 413
            GRWSVFGEVIE ++    E++   K     + + CSN  E+C C++E E C CG  S   
Sbjct: 464  GRWSVFGEVIETINHINDESSLRNKKPSQAKCSPCSNPVESCACTREPEPCPCGPESCGD 523

Query: 412  ----GCGETEEKTVSVSKDDQKSGNYRSQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQE 245
                 CG T  +  SVS++   + +  S+NL+GW LRKRKN  H+  E E+     +KQE
Sbjct: 524  CGPESCGPTTIEESSVSRNSMFAEDLNSRNLIGWLLRKRKNHVHKKVECEIALGSMKKQE 583

Query: 244  -TSSQMYEWALLDKALLVGIIM 182
                 M EW ++DKALLVGI +
Sbjct: 584  WAQGSMGEWGVVDKALLVGIFV 605


>ref|XP_007020083.2| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase
            [Theobroma cacao]
          Length = 623

 Score =  864 bits (2233), Expect = 0.0
 Identities = 431/570 (75%), Positives = 483/570 (84%), Gaps = 4/570 (0%)
 Frame = -3

Query: 1852 DSLKSYNTAKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTC 1673
            D L      KIPGTQTIY+KTFGCSHNQSDSEYMAGQLSAFGY +S+N +E DLWLINTC
Sbjct: 45   DPLTPLLPPKIPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYAISDNPEEGDLWLINTC 104

Query: 1672 TVKSPSQSAMETLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEE 1493
            TVKSPSQSAM TLI KC++AKKPLVVAGCVPQGSRD+KELEGVSIVGVQQIDRVVEVVEE
Sbjct: 105  TVKSPSQSAMNTLITKCKSAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEE 164

Query: 1492 TLKGHEVRLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVD 1313
            TLKGHEVRL+ RKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYT+D
Sbjct: 165  TLKGHEVRLMNRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTID 224

Query: 1312 SLVGRVRTVIADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTN 1133
            SLV RV+TVIADGVKEIWLSSEDTGAYGRDIGVNLPILL++LVAELP DG+TMLRIGMTN
Sbjct: 225  SLVERVKTVIADGVKEIWLSSEDTGAYGRDIGVNLPILLNALVAELPPDGNTMLRIGMTN 284

Query: 1132 PPYILEHLKQIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVP 953
            PP+ILEHLK+IA+VLRHPCVYSFLHVPVQSGSD ILTAMNREYTVSEFRTV DTL ELVP
Sbjct: 285  PPFILEHLKEIADVLRHPCVYSFLHVPVQSGSDAILTAMNREYTVSEFRTVVDTLTELVP 344

Query: 952  GMQIATDIICGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVK 773
            GMQIATDIICGFPGETDEDF QTVNLIKEYKF QVHISQFYPRPGTPAARMKKVPS +VK
Sbjct: 345  GMQIATDIICGFPGETDEDFAQTVNLIKEYKFPQVHISQFYPRPGTPAARMKKVPSTIVK 404

Query: 772  KRSRELTTIFESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTS 593
             RSRELT++FE+FTPY+GMEG++ERIWITEVA DG HLVGHTKGY+QVLV+G ES+LGTS
Sbjct: 405  NRSRELTSVFEAFTPYIGMEGRVERIWITEVAADGIHLVGHTKGYVQVLVIGPESMLGTS 464

Query: 592  AIVKITSVGRWSVFGEVIEILSENANSRKNHGSVEENSCS----NLKETCCSKEQETCAC 425
            AIVKITSVGRWSVFGEVIE L++            +  CS          CSKE E C+C
Sbjct: 465  AIVKITSVGRWSVFGEVIETLTQVNLKMAPEKVCNQEKCSTRFNQFDSGACSKEPEPCSC 524

Query: 424  GLTSGCGETEEKTVSVSKDDQKSGNYRSQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQE 245
            G     G+T  +  +VS +D    +  S+NL+GW LRKR+N  H+  EN +   L +KQ+
Sbjct: 525  GPNCCGGQTILEECTVSSNDSWMEDRSSKNLIGWLLRKRRNHVHKKVENGLALGLRKKQD 584

Query: 244  TSSQMYEWALLDKALLVGIIMSLSTIIAML 155
             +  +  W ++D+ALLVGI++S   I+A++
Sbjct: 585  WA--LGGWGIVDRALLVGILVSFFIIVAVI 612


>ref|XP_019231974.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase
            [Nicotiana attenuata]
 gb|OIT28357.1| cdk5rap1-like protein [Nicotiana attenuata]
          Length = 626

 Score =  864 bits (2233), Expect = 0.0
 Identities = 434/562 (77%), Positives = 494/562 (87%), Gaps = 6/562 (1%)
 Frame = -3

Query: 1825 KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQSA 1646
            KIPGTQTIY+KTFGCSHNQSDSEYMAGQLSAFGY LS+N DEADLW+INTCTVKSPSQSA
Sbjct: 53   KIPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYALSDNPDEADLWMINTCTVKSPSQSA 112

Query: 1645 METLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 1466
            M+TLI+K R+AKKPLVVAGCVPQGSR++KELEGVSIVGVQQIDRVVEVVEETLKGHEVRL
Sbjct: 113  MDTLISKGRSAKKPLVVAGCVPQGSRNLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 172

Query: 1465 LTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVRTV 1286
            LTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRV+ V
Sbjct: 173  LTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKNV 232

Query: 1285 IADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTNPPYILEHLK 1106
            IADGV+EIWLSSEDTGAYGRDIGVNLPILL+++VAELP DGSTMLRIGMTNPPYILEHLK
Sbjct: 233  IADGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPLDGSTMLRIGMTNPPYILEHLK 292

Query: 1105 QIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVPGMQIATDII 926
            +IA+VLRHPCVY+FLHVPVQSGSD++L+AMNREYTV EFR V DTL+ELVPGMQIATDII
Sbjct: 293  EIADVLRHPCVYTFLHVPVQSGSDSVLSAMNREYTVGEFRKVVDTLMELVPGMQIATDII 352

Query: 925  CGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELTTI 746
            CGFPGETDEDF QTV+LIK+YK AQVHISQFYPRPGTPAARMKKVPSNVVK+RSRELTT+
Sbjct: 353  CGFPGETDEDFAQTVDLIKDYKLAQVHISQFYPRPGTPAARMKKVPSNVVKQRSRELTTV 412

Query: 745  FESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTSAIVKITSVG 566
            FESFTPY GMEGK+ERIWIT++A+DG HLVGHTKGYIQVLV+G ES+LG SA+V ITS+G
Sbjct: 413  FESFTPYTGMEGKVERIWITDIASDGIHLVGHTKGYIQVLVIGPESMLGASAMVNITSIG 472

Query: 565  RWSVFGEVIEILSENANSRKNHGSVEE--NSCSNLKETC-CSKEQET-CACGLTSGCGET 398
            RWSVFGEVIEILS+      +H  V+E  + C+N +E C CSKEQE   +CG TS C ++
Sbjct: 473  RWSVFGEVIEILSQINKEVGSHKRVDEKCSPCANSEEACACSKEQEPGSSCGTTSCCSQS 532

Query: 397  E-EKTVSVSKDDQKSGNYRSQNLVGWFLRKRKN-SSHRMKENEMVSKLDEKQETSSQMYE 224
              ++T ++ K++ +  +  S+NL+GWFLR RKN SS +M      SK  ++ +       
Sbjct: 533  PMQETTALLKNEPEQDDQGSRNLIGWFLRNRKNHSSEKMDFAGSESKEKKQIQDLGHQSA 592

Query: 223  WALLDKALLVGIIMSLSTIIAM 158
            W  +D ALL GI++SL TI+A+
Sbjct: 593  WGPVDIALLSGILLSLFTIVAL 614


>ref|XP_016438122.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase-like
            [Nicotiana tabacum]
          Length = 625

 Score =  863 bits (2231), Expect = 0.0
 Identities = 431/561 (76%), Positives = 493/561 (87%), Gaps = 5/561 (0%)
 Frame = -3

Query: 1825 KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQSA 1646
            K+PGTQTIY+KTFGCSHNQSDSEYMAGQLSAFGY LS+N DEADLW+INTCTVKSPSQSA
Sbjct: 53   KLPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYALSDNPDEADLWMINTCTVKSPSQSA 112

Query: 1645 METLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 1466
            M+TLI+K R+AKKPLVVAGCVPQGSR++KELEGVSIVGVQQIDRVVEVVEETLKGHEVRL
Sbjct: 113  MDTLISKGRSAKKPLVVAGCVPQGSRNLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 172

Query: 1465 LTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVRTV 1286
            LTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRV+ V
Sbjct: 173  LTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKNV 232

Query: 1285 IADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTNPPYILEHLK 1106
            IADGV+EIWLSSEDTGAYGRDIGVNLPILL+++VAELP DGSTMLRIGMTNPPYILEHLK
Sbjct: 233  IADGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPLDGSTMLRIGMTNPPYILEHLK 292

Query: 1105 QIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVPGMQIATDII 926
            +IA+VLRHPCVY+FLHVPVQSGSD++L+AMNREYTV EFR V DTL+ELVPGMQIATDII
Sbjct: 293  EIADVLRHPCVYTFLHVPVQSGSDSVLSAMNREYTVGEFRKVVDTLMELVPGMQIATDII 352

Query: 925  CGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELTTI 746
            CGFPGETDEDF QTV+LIK+YK AQVHISQFYPRPGTPAARMKKVPSNVVK+RSRELT +
Sbjct: 353  CGFPGETDEDFAQTVDLIKDYKLAQVHISQFYPRPGTPAARMKKVPSNVVKQRSRELTAV 412

Query: 745  FESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTSAIVKITSVG 566
            FESFTPY GMEGK+ERIWIT++A+DG HLVGHTKGYIQVLV+G ES+LG SA+VKITSVG
Sbjct: 413  FESFTPYTGMEGKVERIWITDIASDGIHLVGHTKGYIQVLVIGPESMLGASAMVKITSVG 472

Query: 565  RWSVFGEVIEILSENANSRKNHGSVEE--NSCSNLKETC-CSKEQET-CACGLTSGCGET 398
            RWSVFGEVIEI+S+N     +H  V+E  + C+N +E C CSKEQE   +   TS C ++
Sbjct: 473  RWSVFGEVIEIISQNNKEVGSHERVDEKCSPCANSEEACACSKEQEPGSSYATTSCCSQS 532

Query: 397  EEKTVSVSKDDQKSGNYRSQNLVGWFLRKRKN-SSHRMKENEMVSKLDEKQETSSQMYEW 221
              +  ++ K++ K  +  S+NL+GWFLR RKN SS +M    + SK  ++ +   +   W
Sbjct: 533  PMQETTLLKNEPKLDDQGSRNLIGWFLRNRKNHSSKKMDLAGLASKEKKQIQDLGRQSAW 592

Query: 220  ALLDKALLVGIIMSLSTIIAM 158
              +D ALL G+++SL TI+A+
Sbjct: 593  GSVDIALLSGVLLSLFTIVAL 613


>ref|XP_011001084.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase-like
            [Populus euphratica]
          Length = 625

 Score =  862 bits (2228), Expect = 0.0
 Identities = 427/565 (75%), Positives = 489/565 (86%), Gaps = 6/565 (1%)
 Frame = -3

Query: 1831 TAKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQ 1652
            ++KIPGTQTIY+KTFGCSHNQSDSEYMAGQLS+FGY+L ++ +EADLWLINTCTVKSPSQ
Sbjct: 50   SSKIPGTQTIYIKTFGCSHNQSDSEYMAGQLSSFGYSLCDSPEEADLWLINTCTVKSPSQ 109

Query: 1651 SAMETLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEETLKGHEV 1472
            SAM+TLI+K ++AKKPLVVAGCVPQGSR++KELEGVSIVGVQQIDRVVEVVEETLKGHEV
Sbjct: 110  SAMDTLISKGKSAKKPLVVAGCVPQGSRNVKELEGVSIVGVQQIDRVVEVVEETLKGHEV 169

Query: 1471 RLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVR 1292
            RLL RKTLPALDLPKVRKNKF+EILPINVGCLGACTYCKTKHARGHLGSYTVDSL GRV+
Sbjct: 170  RLLNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLAGRVK 229

Query: 1291 TVIADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTNPPYILEH 1112
            TVI DGVKEIWLSSEDTGAYGRDIG+NLPILL+++VAELPSD STMLRIGMTNPP+ILEH
Sbjct: 230  TVIDDGVKEIWLSSEDTGAYGRDIGINLPILLNAIVAELPSDASTMLRIGMTNPPFILEH 289

Query: 1111 LKQIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVPGMQIATD 932
            LK+IAEVLRHPCVYSFLHVPVQSGSD ILTAMNREYTV+EFRTV DTLIELVPGMQIATD
Sbjct: 290  LKEIAEVLRHPCVYSFLHVPVQSGSDAILTAMNREYTVNEFRTVVDTLIELVPGMQIATD 349

Query: 931  IICGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELT 752
            IICGFPGETD+DF QTVNLIK YKFAQVHISQFYPRPGTPAARMKKVPSN+VK+RSRELT
Sbjct: 350  IICGFPGETDKDFSQTVNLIKAYKFAQVHISQFYPRPGTPAARMKKVPSNIVKQRSRELT 409

Query: 751  TIFESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTSAIVKITS 572
            T+FE+FTPY GMEG++ERIWIT++ATDG HLVGHTK Y+QVL+V  ES+LGTSAIVKITS
Sbjct: 410  TVFEAFTPYNGMEGRVERIWITDIATDGIHLVGHTKAYVQVLIVAQESMLGTSAIVKITS 469

Query: 571  VGRWSVFGEVIEILSE-NANSRKNHGSVEENSCSNLKETC----CSKEQETCACGLTSGC 407
            VGRWSVFGEVIE L++ N  S+     + E  CS   + C    CS E E CACG  S  
Sbjct: 470  VGRWSVFGEVIETLNQTNQKSKSVEKMLSEEKCSPCSDPCDSCACSGESEPCACGPESCG 529

Query: 406  GETEEKTVSVSKDDQKSGNYRSQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQE-TSSQM 230
            G++  +   V ++D    +   +NL+GW LRK+KN + +M EN + S   ++QE      
Sbjct: 530  GQSTSEQSDVLQNDVLLEDQNRRNLIGWLLRKQKNQARKMVENGIASGSQKRQEWAKGAP 589

Query: 229  YEWALLDKALLVGIIMSLSTIIAML 155
             EW ++D+ALL G+I+S+ T +A+L
Sbjct: 590  EEWGVVDRALLGGLIVSVFTTVALL 614


>ref|XP_021288193.1| threonylcarbamoyladenosine tRNA methylthiotransferase [Herrania
            umbratica]
          Length = 623

 Score =  862 bits (2227), Expect = 0.0
 Identities = 430/570 (75%), Positives = 486/570 (85%), Gaps = 4/570 (0%)
 Frame = -3

Query: 1852 DSLKSYNTAKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTC 1673
            D L +    KIPGTQTIY+KTFGCSHNQSDSEYMAGQLSAFGY +S+N +E DLWLINTC
Sbjct: 45   DPLTALLPPKIPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYAISDNPEEGDLWLINTC 104

Query: 1672 TVKSPSQSAMETLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEE 1493
            TVKSPSQSAM TLI KC++AKKPLVVAGCVPQGSRD+KELEGVSIVGVQQIDRVVEVVEE
Sbjct: 105  TVKSPSQSAMNTLITKCKSAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEE 164

Query: 1492 TLKGHEVRLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVD 1313
            TLKGHEVRL+ RKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYT+D
Sbjct: 165  TLKGHEVRLMNRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTID 224

Query: 1312 SLVGRVRTVIADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTN 1133
            SLVGRV+TVIADGVKEIWLSSEDTGAYGRDIGVNLPILL+++VAELPSDG+TMLRIGMTN
Sbjct: 225  SLVGRVKTVIADGVKEIWLSSEDTGAYGRDIGVNLPILLNAIVAELPSDGNTMLRIGMTN 284

Query: 1132 PPYILEHLKQIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVP 953
            PP+ILEHLK+I  VLRHPCVYSFLHVPVQSGSD ILTAMNREYTV+EFRTV DTL ELVP
Sbjct: 285  PPFILEHLKEIGNVLRHPCVYSFLHVPVQSGSDAILTAMNREYTVTEFRTVVDTLTELVP 344

Query: 952  GMQIATDIICGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVK 773
             MQIATDIICGFPGETDEDF QTVNLIKEYKF QVHISQFYPRPGTPAARMKKVPS +VK
Sbjct: 345  EMQIATDIICGFPGETDEDFAQTVNLIKEYKFPQVHISQFYPRPGTPAARMKKVPSTIVK 404

Query: 772  KRSRELTTIFESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTS 593
             RSRELT++FE+FTPY GMEG++ERIWITE+A DG HLVGHTKGY+QVLV+  ES+LGTS
Sbjct: 405  NRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYVQVLVIAPESMLGTS 464

Query: 592  AIVKITSVGRWSVFGEVIEILSENANSRKNHGSVEENSCS---NLKETC-CSKEQETCAC 425
            AIVKITSVGRWSVFGEVIE L++            +  CS   N  ++C CSKE E C+C
Sbjct: 465  AIVKITSVGRWSVFGEVIETLTQVNLKMAPEKVCNQEKCSPRFNQFDSCACSKEPEPCSC 524

Query: 424  GLTSGCGETEEKTVSVSKDDQKSGNYRSQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQE 245
            G  S  G+T  +  +VS +D    +  S+NL+GW LRKR+N  H+  EN +   L +KQ+
Sbjct: 525  GPNSCGGQTLLEECTVSSNDSWMEDRSSKNLIGWLLRKRRNHEHKKVENGIALGLRKKQD 584

Query: 244  TSSQMYEWALLDKALLVGIIMSLSTIIAML 155
             +  +  W ++D+ALLVGI++S   I+A++
Sbjct: 585  WA--LGGWGIVDRALLVGILVSFLMIVAVI 612


>ref|XP_009587336.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase
            [Nicotiana tomentosiformis]
          Length = 626

 Score =  861 bits (2225), Expect = 0.0
 Identities = 431/562 (76%), Positives = 492/562 (87%), Gaps = 6/562 (1%)
 Frame = -3

Query: 1825 KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQSA 1646
            K+PGTQTIY+KTFGCSHNQSDSEYMAGQLSAFGY LS+N DEADLW+INTCTVKSPSQSA
Sbjct: 53   KLPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYALSDNPDEADLWMINTCTVKSPSQSA 112

Query: 1645 METLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 1466
            M+TLI+K R+AKKPLVVAGCVPQGSR++KELEGVSIVGVQQIDRVVEVVEETLKGHEVRL
Sbjct: 113  MDTLISKGRSAKKPLVVAGCVPQGSRNLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 172

Query: 1465 LTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVRTV 1286
            LTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRV+ V
Sbjct: 173  LTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKNV 232

Query: 1285 IADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTNPPYILEHLK 1106
            IADGV+EIWLSSEDTGAYGRDIGVNLPILL ++VAELP DGSTMLRIGMTNPPYILEHLK
Sbjct: 233  IADGVREIWLSSEDTGAYGRDIGVNLPILLIAIVAELPLDGSTMLRIGMTNPPYILEHLK 292

Query: 1105 QIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVPGMQIATDII 926
            +IA+VLRHPCVY+FLHVPVQSGSD++L+AMNREYTV EFR V DTL+ELVPGMQIATDII
Sbjct: 293  EIADVLRHPCVYTFLHVPVQSGSDSVLSAMNREYTVGEFRKVVDTLMELVPGMQIATDII 352

Query: 925  CGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELTTI 746
            CGFPGETDEDF QTV+LIK+YK AQVHISQFYPRPGTPAARMKKVPSNVVK+RSRELT +
Sbjct: 353  CGFPGETDEDFAQTVDLIKDYKLAQVHISQFYPRPGTPAARMKKVPSNVVKQRSRELTAV 412

Query: 745  FESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTSAIVKITSVG 566
            FESFTPY GMEGK+ERIWIT++A+DG HLVGHTKGYIQVLV+G ES+LG SA+VKITSVG
Sbjct: 413  FESFTPYTGMEGKVERIWITDIASDGIHLVGHTKGYIQVLVIGPESMLGASAMVKITSVG 472

Query: 565  RWSVFGEVIEILSENANSRKNHGSVEE--NSCSNLKETC-CSKEQETCACGLTSGCGETE 395
            RWSVFGEVIEI+S+N     +H  V+E  + C+N +E C CSKEQE  +   T+ C    
Sbjct: 473  RWSVFGEVIEIISQNNKEVGSHERVDEKCSPCANSEEACACSKEQEPGSSYATTSCCSQS 532

Query: 394  --EKTVSVSKDDQKSGNYRSQNLVGWFLRKRKN-SSHRMKENEMVSKLDEKQETSSQMYE 224
              ++T ++ K++ K  +  S+NL+GWFLR RKN SS +M    + SK  ++ +   +   
Sbjct: 533  PMQETTTLLKNEPKLDDQGSRNLIGWFLRNRKNHSSKKMDLAGLESKEKKQIQDLGRQSA 592

Query: 223  WALLDKALLVGIIMSLSTIIAM 158
            W  +D ALL G+++SL TI+A+
Sbjct: 593  WGSVDIALLSGVLLSLFTIVAL 614


>ref|XP_024174573.1| threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1
            [Rosa chinensis]
          Length = 627

 Score =  861 bits (2225), Expect = 0.0
 Identities = 436/573 (76%), Positives = 491/573 (85%), Gaps = 8/573 (1%)
 Frame = -3

Query: 1825 KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQSA 1646
            K+PGTQTIY+KTFGCSHNQSDSEYMAGQLSAFGY+LS+N ++ADLWLINTCTVKSPSQSA
Sbjct: 54   KVPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYSLSDNPEDADLWLINTCTVKSPSQSA 113

Query: 1645 METLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 1466
            M+TLIAK + AKKPLVVAGCVPQGSRD+KEL+GVSIVGVQQIDRVVE+VEETLKGHEVRL
Sbjct: 114  MDTLIAKGKGAKKPLVVAGCVPQGSRDIKELDGVSIVGVQQIDRVVEIVEETLKGHEVRL 173

Query: 1465 LTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVRTV 1286
            L+RKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVD LVGRV++V
Sbjct: 174  LSRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDGLVGRVKSV 233

Query: 1285 IADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTNPPYILEHLK 1106
            IADGV+EIWLSSEDTGAYGRDIGVNLPILL+++V ELP D STMLRIGMTNPP+ILEHLK
Sbjct: 234  IADGVREIWLSSEDTGAYGRDIGVNLPILLNAIVKELPPDASTMLRIGMTNPPFILEHLK 293

Query: 1105 QIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVPGMQIATDII 926
            +IAEVLRHPCVYSFLHVPVQSGSD +LTAMNREYTVSEF+TV DTL ELVPGMQIATDII
Sbjct: 294  EIAEVLRHPCVYSFLHVPVQSGSDAVLTAMNREYTVSEFKTVVDTLTELVPGMQIATDII 353

Query: 925  CGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELTTI 746
            CGFPGETDEDF QT +LIKEYKF QVHISQFYPRPGTPAARMKKVPS VVK RSRELT++
Sbjct: 354  CGFPGETDEDFAQTRDLIKEYKFPQVHISQFYPRPGTPAARMKKVPSTVVKNRSRELTSV 413

Query: 745  FESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTSAIVKITSVG 566
            FE+FTPYVGMEG++ERIWITEVA DG+HLVGHTKGY+QVLV G ES+LGTSAIVKITSVG
Sbjct: 414  FEAFTPYVGMEGRVERIWITEVAADGTHLVGHTKGYVQVLVAGPESMLGTSAIVKITSVG 473

Query: 565  RWSVFGEVIEILSENANSRKNHGSVEENS-----CSNLKETC-CSKEQETCACGLTSGCG 404
            RWSVFGEVIE + E +N +   G   ++      CSN  ETC CS E ETCACG  S CG
Sbjct: 474  RWSVFGEVIETIPE-SNDKAAVGKERQSQENCFPCSNNFETCACSTEPETCACGPES-CG 531

Query: 403  ETEEKT-VSVSKDDQKSGNYRSQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQ-ETSSQM 230
            E    T  SV+       +  ++NL+GW LRKRKN   +  ENE+   L +KQ +T   +
Sbjct: 532  EQATLTKCSVTTTGVPLEDQNNRNLIGWLLRKRKNQVQKFVENEIDLGLKKKQGQTQESI 591

Query: 229  YEWALLDKALLVGIIMSLSTIIAMLCIWIYRFL 131
             +W ++DKALL G++ S  TI+A+L    +R +
Sbjct: 592  GKWGVVDKALLGGMLASFLTIVAVLAHLGFRIM 624


>ref|XP_006350322.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase
            isoform X1 [Solanum tuberosum]
          Length = 622

 Score =  860 bits (2222), Expect = 0.0
 Identities = 432/562 (76%), Positives = 491/562 (87%), Gaps = 6/562 (1%)
 Frame = -3

Query: 1825 KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQSA 1646
            KIPGTQTIY+KTFGCSHNQSDSEYMAGQLSAFGY LS+N DEADLW+INTCTVKSPSQSA
Sbjct: 52   KIPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYALSDNPDEADLWMINTCTVKSPSQSA 111

Query: 1645 METLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 1466
            M+T+I+K R+AKKPLVVAGCVPQGSR++KELEGVSIVGVQQIDRVVEVVEETLKGHEVRL
Sbjct: 112  MDTIISKGRSAKKPLVVAGCVPQGSRNLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 171

Query: 1465 LTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVRTV 1286
            LTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRV+ V
Sbjct: 172  LTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKNV 231

Query: 1285 IADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTNPPYILEHLK 1106
            IADGV+EIWLSSEDTGAYGRDIGVNLPILL+++VAELP +GSTMLRIGMTNPPYILEHLK
Sbjct: 232  IADGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPLNGSTMLRIGMTNPPYILEHLK 291

Query: 1105 QIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVPGMQIATDII 926
            +IA+VL HPCVYSFLHVPVQSGSD+IL+AMNREYTV EFR V DTL+ELVPGMQIATDII
Sbjct: 292  EIADVLCHPCVYSFLHVPVQSGSDSILSAMNREYTVGEFRKVVDTLMELVPGMQIATDII 351

Query: 925  CGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELTTI 746
            CGFPGETDEDF QTV+LIK+YK AQVHISQFYPRPGTPAARMKKVPSNVVK+RSRELT +
Sbjct: 352  CGFPGETDEDFAQTVDLIKDYKLAQVHISQFYPRPGTPAARMKKVPSNVVKQRSRELTAV 411

Query: 745  FESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTSAIVKITSVG 566
            FESFTPY G+EGK+ERIWIT++A+DG HLVGHTKGYIQVLV+G ES++G+SA+VKITSVG
Sbjct: 412  FESFTPYTGVEGKVERIWITDIASDGIHLVGHTKGYIQVLVIGPESMMGSSAMVKITSVG 471

Query: 565  RWSVFGEVIEILSENANSRKNHGSVEE--NSCSNLKETC-CSKEQETCACGLTSGC---G 404
            RWSVFGEVIEIL +N     +H S +E  + C+  +E C CSKEQE C+   TS C    
Sbjct: 472  RWSVFGEVIEILFQNNEDVPSHRSFDERCSPCALSEEDCACSKEQEPCSA--TSDCCNQS 529

Query: 403  ETEEKTVSVSKDDQKSGNYRSQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQETSSQMYE 224
             T E T ++ K+D    N+ S+NL+GWFLR RKN S +  +       +++ +  S    
Sbjct: 530  PTREAT-TLLKNDPNQDNHSSRNLIGWFLRNRKNQSSKKMDLTRSESKEKQIQDLSHRSA 588

Query: 223  WALLDKALLVGIIMSLSTIIAM 158
            W+L+D ALL GI++SL TI+A+
Sbjct: 589  WSLVDIALLSGILLSLFTIVAL 610


>gb|PNT07771.1| hypothetical protein POPTR_013G107800v3 [Populus trichocarpa]
          Length = 625

 Score =  860 bits (2222), Expect = 0.0
 Identities = 428/565 (75%), Positives = 489/565 (86%), Gaps = 6/565 (1%)
 Frame = -3

Query: 1831 TAKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQ 1652
            ++KIPGTQTIY+KTFGCSHNQSDSEYMAGQLS+FGY+LS++ +EADLWLINTCTVKSPSQ
Sbjct: 50   SSKIPGTQTIYIKTFGCSHNQSDSEYMAGQLSSFGYSLSDSPEEADLWLINTCTVKSPSQ 109

Query: 1651 SAMETLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEETLKGHEV 1472
            SAM+TLI+K ++AKKPLVVAGCVPQGSR++KELEGVSIVGVQQIDRVVEVVEETLKGHEV
Sbjct: 110  SAMDTLISKGKSAKKPLVVAGCVPQGSRNVKELEGVSIVGVQQIDRVVEVVEETLKGHEV 169

Query: 1471 RLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVR 1292
            RLL RKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTV+SL GRV+
Sbjct: 170  RLLNRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVNSLAGRVK 229

Query: 1291 TVIADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTNPPYILEH 1112
            TVI DGVKEIWLSSEDTGAYGRDIGVNLPILL+++VAELPSDGSTMLRIGMTNPP+ILEH
Sbjct: 230  TVIDDGVKEIWLSSEDTGAYGRDIGVNLPILLNAIVAELPSDGSTMLRIGMTNPPFILEH 289

Query: 1111 LKQIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVPGMQIATD 932
            LK+IAEVLRHPCVYSFLHVPVQSGSD ILTAMNREYTV+EFRTV DTL ELVPGMQIATD
Sbjct: 290  LKEIAEVLRHPCVYSFLHVPVQSGSDAILTAMNREYTVNEFRTVVDTLTELVPGMQIATD 349

Query: 931  IICGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELT 752
            IICGFPGETD+DF QTVNLIK YKFAQVHISQFYPRPGTPAARMKKVPSN+VK+RSRELT
Sbjct: 350  IICGFPGETDKDFSQTVNLIKAYKFAQVHISQFYPRPGTPAARMKKVPSNIVKQRSRELT 409

Query: 751  TIFESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTSAIVKITS 572
            ++FE+FTPY GMEG++ERIWIT++A DG HLVGHTK Y+QVL+V  ES+LGTSAIVKITS
Sbjct: 410  SVFEAFTPYNGMEGRVERIWITDIAADGIHLVGHTKAYVQVLIVAQESMLGTSAIVKITS 469

Query: 571  VGRWSVFGEVIEILSE-NANSRKNHGSVEENSCSNLKETC----CSKEQETCACGLTSGC 407
            VGRWSVFGEVIE L++ N  S+     + E  CS   + C    CS E E CACG  S  
Sbjct: 470  VGRWSVFGEVIETLNQINQKSKSVEKMLSEEKCSPCSDPCDSCACSGESEPCACGPESCG 529

Query: 406  GETEEKTVSVSKDDQKSGNYRSQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQE-TSSQM 230
            G++  +   V +++    +   +NL+GW LRKRKN + +M EN + S   +KQE      
Sbjct: 530  GQSTIEQSDVLQNEVLREDQNRRNLIGWLLRKRKNQAQKMVENGIASGSQKKQEWAKGAP 589

Query: 229  YEWALLDKALLVGIIMSLSTIIAML 155
             EW ++D+ALL G+I+S+ T +A+L
Sbjct: 590  EEWGVVDRALLGGLIVSVFTTVAIL 614


>gb|PRQ20424.1| putative tRNA
            (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
            methylthiotransferase [Rosa chinensis]
          Length = 659

 Score =  861 bits (2225), Expect = 0.0
 Identities = 436/573 (76%), Positives = 491/573 (85%), Gaps = 8/573 (1%)
 Frame = -3

Query: 1825 KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQSA 1646
            K+PGTQTIY+KTFGCSHNQSDSEYMAGQLSAFGY+LS+N ++ADLWLINTCTVKSPSQSA
Sbjct: 86   KVPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYSLSDNPEDADLWLINTCTVKSPSQSA 145

Query: 1645 METLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 1466
            M+TLIAK + AKKPLVVAGCVPQGSRD+KEL+GVSIVGVQQIDRVVE+VEETLKGHEVRL
Sbjct: 146  MDTLIAKGKGAKKPLVVAGCVPQGSRDIKELDGVSIVGVQQIDRVVEIVEETLKGHEVRL 205

Query: 1465 LTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVRTV 1286
            L+RKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVD LVGRV++V
Sbjct: 206  LSRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDGLVGRVKSV 265

Query: 1285 IADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTNPPYILEHLK 1106
            IADGV+EIWLSSEDTGAYGRDIGVNLPILL+++V ELP D STMLRIGMTNPP+ILEHLK
Sbjct: 266  IADGVREIWLSSEDTGAYGRDIGVNLPILLNAIVKELPPDASTMLRIGMTNPPFILEHLK 325

Query: 1105 QIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVPGMQIATDII 926
            +IAEVLRHPCVYSFLHVPVQSGSD +LTAMNREYTVSEF+TV DTL ELVPGMQIATDII
Sbjct: 326  EIAEVLRHPCVYSFLHVPVQSGSDAVLTAMNREYTVSEFKTVVDTLTELVPGMQIATDII 385

Query: 925  CGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELTTI 746
            CGFPGETDEDF QT +LIKEYKF QVHISQFYPRPGTPAARMKKVPS VVK RSRELT++
Sbjct: 386  CGFPGETDEDFAQTRDLIKEYKFPQVHISQFYPRPGTPAARMKKVPSTVVKNRSRELTSV 445

Query: 745  FESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTSAIVKITSVG 566
            FE+FTPYVGMEG++ERIWITEVA DG+HLVGHTKGY+QVLV G ES+LGTSAIVKITSVG
Sbjct: 446  FEAFTPYVGMEGRVERIWITEVAADGTHLVGHTKGYVQVLVAGPESMLGTSAIVKITSVG 505

Query: 565  RWSVFGEVIEILSENANSRKNHGSVEENS-----CSNLKETC-CSKEQETCACGLTSGCG 404
            RWSVFGEVIE + E +N +   G   ++      CSN  ETC CS E ETCACG  S CG
Sbjct: 506  RWSVFGEVIETIPE-SNDKAAVGKERQSQENCFPCSNNFETCACSTEPETCACGPES-CG 563

Query: 403  ETEEKT-VSVSKDDQKSGNYRSQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQ-ETSSQM 230
            E    T  SV+       +  ++NL+GW LRKRKN   +  ENE+   L +KQ +T   +
Sbjct: 564  EQATLTKCSVTTTGVPLEDQNNRNLIGWLLRKRKNQVQKFVENEIDLGLKKKQGQTQESI 623

Query: 229  YEWALLDKALLVGIIMSLSTIIAMLCIWIYRFL 131
             +W ++DKALL G++ S  TI+A+L    +R +
Sbjct: 624  GKWGVVDKALLGGMLASFLTIVAVLAHLGFRIM 656


>ref|XP_004250429.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase
            [Solanum lycopersicum]
          Length = 622

 Score =  858 bits (2218), Expect = 0.0
 Identities = 432/564 (76%), Positives = 491/564 (87%), Gaps = 6/564 (1%)
 Frame = -3

Query: 1831 TAKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQ 1652
            T KIPGTQTIY+KTFGCSHNQSDSEYMAGQLSAFGY LS+N DEADLW+INTCTVKSPSQ
Sbjct: 50   TPKIPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYALSDNPDEADLWMINTCTVKSPSQ 109

Query: 1651 SAMETLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEETLKGHEV 1472
            SAM+T+I+K R+AKKPLVVAGCVPQGSR++K+LEGVSIVGVQQIDRVVEVVEETLKGHEV
Sbjct: 110  SAMDTIISKGRSAKKPLVVAGCVPQGSRNLKDLEGVSIVGVQQIDRVVEVVEETLKGHEV 169

Query: 1471 RLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVR 1292
            RLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRV+
Sbjct: 170  RLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVK 229

Query: 1291 TVIADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTNPPYILEH 1112
             VIADGV+EIWLSSEDTGAYGRDIGVNLPILL+++VA+LP +GSTMLRIGMTNPPYILEH
Sbjct: 230  NVIADGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAQLPLNGSTMLRIGMTNPPYILEH 289

Query: 1111 LKQIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVPGMQIATD 932
            LK+IA+VL HPCVYSFLHVPVQSGSD++L+AMNREYTV EFR V DTL+ELVPGMQIATD
Sbjct: 290  LKEIADVLCHPCVYSFLHVPVQSGSDSVLSAMNREYTVGEFRKVVDTLMELVPGMQIATD 349

Query: 931  IICGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELT 752
            IICGFPGETDEDF QTV+LIK+YK AQVHISQFYPRPGTPAARMKKVPSNVVK+RSRELT
Sbjct: 350  IICGFPGETDEDFAQTVDLIKDYKLAQVHISQFYPRPGTPAARMKKVPSNVVKQRSRELT 409

Query: 751  TIFESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTSAIVKITS 572
             +FESFTPY G+EGK+ERIWIT+VA+DG HLVGHTKGYIQVLV+G ES+LG+SA+VKITS
Sbjct: 410  AVFESFTPYTGVEGKVERIWITDVASDGVHLVGHTKGYIQVLVIGPESMLGSSAMVKITS 469

Query: 571  VGRWSVFGEVIEILSENANSRKNHGSVEE--NSCSNLKETC-CSKEQETCACGLTSGC-- 407
            VGRWSVFGEVIEIL +N     +H S +E  + C+  +E C CSKEQE C+   TS C  
Sbjct: 470  VGRWSVFGEVIEILCQNDKDVPSHRSFDERCSPCALSEEACACSKEQEPCSA--TSDCCN 527

Query: 406  -GETEEKTVSVSKDDQKSGNYRSQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQETSSQM 230
               T E T ++ K+D    N+ S+NL+GWFLR RKN S +  +       ++  +  S  
Sbjct: 528  QSPTVEAT-TLLKNDPNLDNHSSRNLIGWFLRNRKNQSSKKMDLTSSESKEKPIQDLSHR 586

Query: 229  YEWALLDKALLVGIIMSLSTIIAM 158
              W+L+D ALL GI++SL TI+A+
Sbjct: 587  SAWSLVDIALLSGILLSLFTIVAL 610


>ref|XP_015058303.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase
            [Solanum pennellii]
          Length = 622

 Score =  857 bits (2215), Expect = 0.0
 Identities = 433/564 (76%), Positives = 490/564 (86%), Gaps = 6/564 (1%)
 Frame = -3

Query: 1831 TAKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYNLSENQDEADLWLINTCTVKSPSQ 1652
            T KIPGTQTIY+KTFGCSHNQSDSEYMAGQLSAFGY LS+N DEADLW+INTCTVKSPSQ
Sbjct: 50   TPKIPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYALSDNPDEADLWMINTCTVKSPSQ 109

Query: 1651 SAMETLIAKCRNAKKPLVVAGCVPQGSRDMKELEGVSIVGVQQIDRVVEVVEETLKGHEV 1472
            SAM+T+I+K R+AKKPLVVAGCVPQGSR++KELEGVSIVGVQQIDRVVEVVEETLKGHEV
Sbjct: 110  SAMDTIISKGRSAKKPLVVAGCVPQGSRNLKELEGVSIVGVQQIDRVVEVVEETLKGHEV 169

Query: 1471 RLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVR 1292
            RLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RV+
Sbjct: 170  RLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVCRVK 229

Query: 1291 TVIADGVKEIWLSSEDTGAYGRDIGVNLPILLSSLVAELPSDGSTMLRIGMTNPPYILEH 1112
             VIADGV+EIWLSSEDTGAYGRDIGVNLPILL+++VAELP +GSTMLRIGMTNPPYILEH
Sbjct: 230  NVIADGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPLNGSTMLRIGMTNPPYILEH 289

Query: 1111 LKQIAEVLRHPCVYSFLHVPVQSGSDTILTAMNREYTVSEFRTVADTLIELVPGMQIATD 932
            LK+IA+VL HPCVYSFLHVPVQSGSD++L+AMNREYTV EFR V DTL+ELVPGMQIATD
Sbjct: 290  LKEIADVLCHPCVYSFLHVPVQSGSDSVLSAMNREYTVGEFRKVVDTLMELVPGMQIATD 349

Query: 931  IICGFPGETDEDFEQTVNLIKEYKFAQVHISQFYPRPGTPAARMKKVPSNVVKKRSRELT 752
            IICGFPGETDEDF QTV+LIK+YK AQVHISQFYPRPGTPAARMKKVPSNVVK+RSRELT
Sbjct: 350  IICGFPGETDEDFAQTVDLIKDYKLAQVHISQFYPRPGTPAARMKKVPSNVVKQRSRELT 409

Query: 751  TIFESFTPYVGMEGKIERIWITEVATDGSHLVGHTKGYIQVLVVGSESLLGTSAIVKITS 572
             +FESFTPY G+EGK+ERIWIT+VA+DG HLVGHTKGYIQVLV+G ES+LG+SA+VKITS
Sbjct: 410  AVFESFTPYTGVEGKVERIWITDVASDGVHLVGHTKGYIQVLVIGPESMLGSSAMVKITS 469

Query: 571  VGRWSVFGEVIEILSENANSRKNHGSVEE--NSCSNLKETC-CSKEQETCACGLTSGC-- 407
            VGRWSVFGEVIEIL +N     +H S +E  + C+  +E C CSKEQE C+   TS C  
Sbjct: 470  VGRWSVFGEVIEILCQNDKDVPSHWSFDERCSPCTLSEEACACSKEQEPCSA--TSDCCN 527

Query: 406  -GETEEKTVSVSKDDQKSGNYRSQNLVGWFLRKRKNSSHRMKENEMVSKLDEKQETSSQM 230
               T E T ++ K+D    N+ S+NL+GWFLR RKN S +  +       ++  +  S  
Sbjct: 528  QSPTVEAT-TLLKNDPNLDNHSSRNLIGWFLRNRKNQSSKKMDLTSSESKEKPIQDLSHW 586

Query: 229  YEWALLDKALLVGIIMSLSTIIAM 158
              W+L+D ALL GI++SL TI+A+
Sbjct: 587  SAWSLVDIALLSGILLSLFTIVAL 610