BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia31_contig00031030
         (453 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011093631.1| DNA topoisomerase 1 [Sesamum indicum] >gi|11...   127   6e-40
gb|PIN13662.1| DNA topoisomerase I [Handroanthus impetiginosus]       124   2e-36
gb|EYU30724.1| hypothetical protein MIMGU_mgv1a001121mg [Erythra...    97   1e-32
ref|XP_012845487.1| PREDICTED: DNA topoisomerase 1-like [Erythra...    97   2e-32
ref|XP_022846035.1| DNA topoisomerase 1 beta-like [Olea europaea...   107   3e-27
ref|XP_022866226.1| DNA topoisomerase 1 alpha-like, partial [Ole...   107   8e-26
dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]               105   2e-23
gb|OVA14492.1| DNA topoisomerase I [Macleaya cordata]                 101   4e-22
gb|KZV21640.1| DNA topoisomerase 1-like [Dorcoceras hygrometricum]     95   6e-22
ref|XP_019162797.1| PREDICTED: DNA topoisomerase 1 beta-like [Ip...    97   1e-21
ref|XP_009619726.2| PREDICTED: DNA topoisomerase 1-like [Nicotia...    99   5e-21
gb|OVA16320.1| DNA topoisomerase I [Macleaya cordata]                  98   7e-21
ref|XP_010266803.1| PREDICTED: DNA topoisomerase 1 alpha-like [N...    94   4e-20
ref|XP_008245865.1| PREDICTED: DNA topoisomerase 1-like, partial...    96   5e-20
gb|PIA50017.1| hypothetical protein AQUCO_01300624v1 [Aquilegia ...    96   5e-20
ref|XP_018824786.1| PREDICTED: DNA topoisomerase 1-like [Juglans...    90   9e-20
ref|XP_016480589.1| PREDICTED: DNA topoisomerase 1-like isoform ...    94   2e-19
ref|XP_016480590.1| PREDICTED: DNA topoisomerase 1-like isoform ...    94   2e-19
dbj|BAG31377.1| topoisomerase I [Catharanthus roseus]                  94   2e-19
ref|XP_021812936.1| DNA topoisomerase 1 beta-like [Prunus avium]       93   5e-19

>ref|XP_011093631.1| DNA topoisomerase 1 [Sesamum indicum]
 ref|XP_020553411.1| DNA topoisomerase 1 [Sesamum indicum]
          Length = 893

 Score =  127 bits (318), Expect(2) = 6e-40
 Identities = 65/105 (61%), Positives = 74/105 (70%)
 Frame = -1

Query: 315 SYKKKPKISDASTSNKNKEASAKSEVKAEDEEDHVPIAQRMKKPTFADKKSSSAKKVTKH 136
           SY KKPK+SDAST NK KE S K E+K ED+EDHVPI+QR+K+ T +DKKSSSAK +TK 
Sbjct: 255 SYLKKPKLSDASTPNKTKETSIKDEMKDEDDEDHVPISQRIKQQTSSDKKSSSAKNMTKP 314

Query: 135 XXXXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
                               YS+SSKVPPSSGEGQKWTTLVHNGV
Sbjct: 315 ISASAKKTSKKPKKVMKNSKYSKSSKVPPSSGEGQKWTTLVHNGV 359



 Score = 65.1 bits (157), Expect(2) = 6e-40
 Identities = 33/50 (66%), Positives = 39/50 (78%)
 Frame = -2

Query: 452 SRSSHSEGKKSFLVKADQNGSASIERTPSSILKKRPADGVASAGQSHIKK 303
           +R S S  +K  L K DQNGSAS ER PS+ILKKRPADGV SAG+S++KK
Sbjct: 210 NRPSISGERKPLLAKYDQNGSASAERKPSTILKKRPADGVKSAGESYLKK 259


>gb|PIN13662.1| DNA topoisomerase I [Handroanthus impetiginosus]
          Length = 895

 Score =  124 bits (310), Expect(2) = 2e-36
 Identities = 62/105 (59%), Positives = 73/105 (69%)
 Frame = -1

Query: 315 SYKKKPKISDASTSNKNKEASAKSEVKAEDEEDHVPIAQRMKKPTFADKKSSSAKKVTKH 136
           S+ K+PK+SDAS SNKNKE S K+E+K ED+EDHVPI+ RMKK T +DKK SS+K +TK 
Sbjct: 257 SHLKRPKLSDASASNKNKETSIKAELKEEDDEDHVPISARMKKSTSSDKKPSSSKNITKP 316

Query: 135 XXXXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
                               YS+SSKVPP SGEGQKWTTLVHNGV
Sbjct: 317 VSSLSKKINKKSKKVTKKSEYSKSSKVPPGSGEGQKWTTLVHNGV 361



 Score = 56.2 bits (134), Expect(2) = 2e-36
 Identities = 30/51 (58%), Positives = 35/51 (68%), Gaps = 2/51 (3%)
 Frame = -2

Query: 449 RSSHSEGKKSFLVKADQNGSASIERTPSS--ILKKRPADGVASAGQSHIKK 303
           +S  S G+K    K D NG  S ER PSS  ILKKRPADGV SAG+SH+K+
Sbjct: 211 KSPTSGGRKPLSAKCDHNGLGSPERKPSSSTILKKRPADGVKSAGESHLKR 261


>gb|EYU30724.1| hypothetical protein MIMGU_mgv1a001121mg [Erythranthe guttata]
          Length = 883

 Score = 97.4 bits (241), Expect(2) = 1e-32
 Identities = 55/103 (53%), Positives = 67/103 (65%), Gaps = 1/103 (0%)
 Frame = -1

Query: 306 KKPKISDASTSNKNKEASAKSEVKAEDEEDHVPIAQRMKKPTFADKKSSSAKKVT-KHXX 130
           KKPK+SD S ++K+K+AS K EVKAED+ED VP++QR KK T  +KK S A K   K   
Sbjct: 248 KKPKLSD-SLASKDKKASVKPEVKAEDDEDLVPLSQRTKKSTTPEKKPSPALKTNNKTNS 306

Query: 129 XXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
                             YS+SSK+PPSSG+GQKWTTLVHNGV
Sbjct: 307 KTNSKTNKKSKQVSKDNNYSKSSKLPPSSGDGQKWTTLVHNGV 349



 Score = 70.1 bits (170), Expect(2) = 1e-32
 Identities = 36/51 (70%), Positives = 43/51 (84%), Gaps = 1/51 (1%)
 Frame = -2

Query: 452 SRSSHSEGKKSFLV-KADQNGSASIERTPSSILKKRPADGVASAGQSHIKK 303
           SRSSHSE KK  +  K D+NGSAS ER PS++L+KRPADGVASAGQS++KK
Sbjct: 199 SRSSHSEEKKPLVARKPDKNGSASTERKPSTVLQKRPADGVASAGQSNLKK 249


>ref|XP_012845487.1| PREDICTED: DNA topoisomerase 1-like [Erythranthe guttata]
          Length = 894

 Score = 97.1 bits (240), Expect(2) = 2e-32
 Identities = 57/114 (50%), Positives = 69/114 (60%), Gaps = 12/114 (10%)
 Frame = -1

Query: 306 KKPKISDASTSNKNKEASAKSEVKAEDEEDHVPIAQRMKKPTFADKKSSSAKKVTKH--- 136
           KKPK+SD S ++K+K+AS K EVKAED+ED VP++QR KK T  +KK S A KVTK    
Sbjct: 248 KKPKLSD-SLASKDKKASVKPEVKAEDDEDLVPLSQRTKKSTTPEKKPSPALKVTKSTAS 306

Query: 135 ---------XXXXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
                                        YS+SSK+PPSSG+GQKWTTLVHNGV
Sbjct: 307 ISKKTNNKTNSKTNSKTNKKSKQVSKDNNYSKSSKLPPSSGDGQKWTTLVHNGV 360



 Score = 70.1 bits (170), Expect(2) = 2e-32
 Identities = 36/51 (70%), Positives = 43/51 (84%), Gaps = 1/51 (1%)
 Frame = -2

Query: 452 SRSSHSEGKKSFLV-KADQNGSASIERTPSSILKKRPADGVASAGQSHIKK 303
           SRSSHSE KK  +  K D+NGSAS ER PS++L+KRPADGVASAGQS++KK
Sbjct: 199 SRSSHSEEKKPLVARKPDKNGSASTERKPSTVLQKRPADGVASAGQSNLKK 249


>ref|XP_022846035.1| DNA topoisomerase 1 beta-like [Olea europaea var. sylvestris]
          Length = 771

 Score =  107 bits (268), Expect(2) = 3e-27
 Identities = 57/103 (55%), Positives = 68/103 (66%), Gaps = 1/103 (0%)
 Frame = -1

Query: 306 KKPKISDASTSNKNKEASAKSEVKAEDEED-HVPIAQRMKKPTFADKKSSSAKKVTKHXX 130
           KK KIS+AS    NK+AS K+E K ED++D H+PI+QRMKKP  +DKK S+ KKVTK   
Sbjct: 135 KKHKISNASVLINNKQASVKAEPKVEDDDDDHIPISQRMKKPAASDKKISAIKKVTKVVS 194

Query: 129 XXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
                             YS+SSKVPP SGEGQKWTTL+HNGV
Sbjct: 195 SSLNKTNKNSKKVVKKSQYSKSSKVPPGSGEGQKWTTLIHNGV 237



 Score = 42.0 bits (97), Expect(2) = 3e-27
 Identities = 26/52 (50%), Positives = 31/52 (59%), Gaps = 3/52 (5%)
 Frame = -2

Query: 449 RSSHSEGKKSFLVKADQNGSA---SIERTPSSILKKRPADGVASAGQSHIKK 303
           +S  S+ K     K +QNGS    S  + PS+IL KRPA  V SAGQS IKK
Sbjct: 85  KSYDSDEKMHLAAKNNQNGSPLQDSGSKKPSTILSKRPASEVNSAGQSSIKK 136


>ref|XP_022866226.1| DNA topoisomerase 1 alpha-like, partial [Olea europaea var.
           sylvestris]
          Length = 360

 Score =  107 bits (266), Expect(2) = 8e-26
 Identities = 55/102 (53%), Positives = 66/102 (64%)
 Frame = -1

Query: 306 KKPKISDASTSNKNKEASAKSEVKAEDEEDHVPIAQRMKKPTFADKKSSSAKKVTKHXXX 127
           KK K+S AS    NK+AS K+E K ED++DHVPI+QR+K P  +DKKSS+ KKVTK    
Sbjct: 201 KKHKLSVASVPINNKQASVKAEQKVEDDDDHVPISQRLKIPAASDKKSSAVKKVTKVVSS 260

Query: 126 XXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
                            Y +SSKVPP SGEGQKWTTL+HNGV
Sbjct: 261 SLNKTNKKSKKVMKQSQYIKSSKVPPGSGEGQKWTTLIHNGV 302



 Score = 37.7 bits (86), Expect(2) = 8e-26
 Identities = 22/53 (41%), Positives = 32/53 (60%), Gaps = 3/53 (5%)
 Frame = -2

Query: 452 SRSSHSEGKKSFLVKADQNGSASIE---RTPSSILKKRPADGVASAGQSHIKK 303
           S+S  S+ +K    K ++NGSA  +     PS++L KR A  V SAGQ+ +KK
Sbjct: 150 SKSYDSDEEKPLAAKNNENGSAVRDSGLNKPSTVLSKRSASEVKSAGQASMKK 202


>dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]
          Length = 894

 Score =  105 bits (262), Expect = 2e-23
 Identities = 59/104 (56%), Positives = 70/104 (67%), Gaps = 2/104 (1%)
 Frame = -1

Query: 306 KKPKISDASTSNKNKEASAKSEVKAED-EEDHVPIAQRMKKPTFADKKSSSAK-KVTKHX 133
           KKPK+SDAST   NK+AS K+E KA+D ++DHVPI+QRMKK   +  KSS+AK KVTK  
Sbjct: 257 KKPKLSDASTPVNNKQASKKAEPKADDSDDDHVPISQRMKKSATSGNKSSTAKQKVTKVV 316

Query: 132 XXXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
                              YS+SSKVPPSSGEG+KW TLVHNGV
Sbjct: 317 SSSMKKASKKFKKVVKKSKYSKSSKVPPSSGEGKKWNTLVHNGV 360


>gb|OVA14492.1| DNA topoisomerase I [Macleaya cordata]
          Length = 893

 Score =  101 bits (252), Expect = 4e-22
 Identities = 58/114 (50%), Positives = 70/114 (61%), Gaps = 2/114 (1%)
 Frame = -1

Query: 336 CSVCRSI-SYKKKPKISDASTSNKNKEASAKSEVKAEDEEDHVPIAQRMKKPTFADKKSS 160
           C +  S+ S  KKPK+SDAS S K K+ S K+E K ED++D VPIAQRMKK   +D KS 
Sbjct: 246 CDINTSVHSSVKKPKLSDASVSAKVKQVSVKAEPKKEDDDDDVPIAQRMKKSATSDSKSQ 305

Query: 159 SAKK-VTKHXXXXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
           S KK +TK                     YS+S+K+ PSSGEGQKWTTL HNGV
Sbjct: 306 SMKKTITKVVSSSMKKTSKKSKKVMKNSKYSKSTKLQPSSGEGQKWTTLEHNGV 359


>gb|KZV21640.1| DNA topoisomerase 1-like [Dorcoceras hygrometricum]
          Length = 870

 Score = 95.1 bits (235), Expect(2) = 6e-22
 Identities = 55/109 (50%), Positives = 69/109 (63%)
 Frame = -1

Query: 327 CRSISYKKKPKISDASTSNKNKEASAKSEVKAEDEEDHVPIAQRMKKPTFADKKSSSAKK 148
           CRS   +KKPK+S+AS+      A  +++ KAED++DHVPI+QRMK  + ++KKS S KK
Sbjct: 236 CRSP--EKKPKLSNASS------AIPRAKPKAEDDDDHVPISQRMKNQSSSEKKSLSVKK 287

Query: 147 VTKHXXXXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
           VTK                     YS SSKV P+SGEGQKWTTLVHNGV
Sbjct: 288 VTKSVSAVATKTNKISKKIVKNTKYSISSKVLPNSGEGQKWTTLVHNGV 336



 Score = 36.6 bits (83), Expect(2) = 6e-22
 Identities = 23/52 (44%), Positives = 32/52 (61%), Gaps = 2/52 (3%)
 Frame = -2

Query: 452 SRSSHSEGKKSFLVKADQNGSASIERTPS--SILKKRPADGVASAGQSHIKK 303
           SRS++S+ +K  L KADQNGS+S + +    SI  KRP + V    +S  KK
Sbjct: 191 SRSNNSDERKPILNKADQNGSSSTDHSQKKPSIAMKRPPNPVKVECRSPEKK 242


>ref|XP_019162797.1| PREDICTED: DNA topoisomerase 1 beta-like [Ipomoea nil]
          Length = 887

 Score = 97.1 bits (240), Expect(2) = 1e-21
 Identities = 50/102 (49%), Positives = 61/102 (59%)
 Frame = -1

Query: 306 KKPKISDASTSNKNKEASAKSEVKAEDEEDHVPIAQRMKKPTFADKKSSSAKKVTKHXXX 127
           KKP++SD S S K+K+ S K E   +D+ED++PI+QR+KK +    KS S KK T     
Sbjct: 252 KKPRLSDTSVSAKSKQPSVKEEKNEDDDEDNIPISQRIKKSSTPVNKSKSTKKATAVVSS 311

Query: 126 XXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
                            YSQ SKVPPSSGE QKWTTLVHNGV
Sbjct: 312 SHKIIKKKFKKVKKNSKYSQPSKVPPSSGEAQKWTTLVHNGV 353



 Score = 33.9 bits (76), Expect(2) = 1e-21
 Identities = 20/48 (41%), Positives = 27/48 (56%)
 Frame = -2

Query: 446 SSHSEGKKSFLVKADQNGSASIERTPSSILKKRPADGVASAGQSHIKK 303
           S  ++  K  L K +QNGSA     PS++  KR    + SAGQS +KK
Sbjct: 211 SDTNKDNKPVLPKCEQNGSA-----PSTVPSKRSPVEIKSAGQSSVKK 253


>ref|XP_009619726.2| PREDICTED: DNA topoisomerase 1-like [Nicotiana tomentosiformis]
 ref|XP_018631669.1| PREDICTED: DNA topoisomerase 1-like [Nicotiana tomentosiformis]
          Length = 877

 Score = 98.6 bits (244), Expect(2) = 5e-21
 Identities = 54/102 (52%), Positives = 65/102 (63%)
 Frame = -1

Query: 306 KKPKISDASTSNKNKEASAKSEVKAEDEEDHVPIAQRMKKPTFADKKSSSAKKVTKHXXX 127
           KKP++SDAST   +K+AS K E K ED++D VPI+QR+KK   +  K SSAKK TK    
Sbjct: 247 KKPRLSDASTPVSSKQASVKPEKKTEDDDDDVPISQRIKKSVASASKLSSAKKATK---- 302

Query: 126 XXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
                            YS+SSK+ PSSGEGQKWTTLVHNGV
Sbjct: 303 -VVSSSMKTKKKLKKSKYSKSSKLQPSSGEGQKWTTLVHNGV 343



 Score = 30.0 bits (66), Expect(2) = 5e-21
 Identities = 21/50 (42%), Positives = 28/50 (56%)
 Frame = -2

Query: 452 SRSSHSEGKKSFLVKADQNGSASIERTPSSILKKRPADGVASAGQSHIKK 303
           S+SS+SE  K    K  QNG  S     +++L KRP   V SA Q+ +KK
Sbjct: 207 SKSSNSEEVKP---KIRQNGLPS-----TTVLSKRPPGDVKSAAQASVKK 248


>gb|OVA16320.1| DNA topoisomerase I [Macleaya cordata]
          Length = 725

 Score = 98.2 bits (243), Expect = 7e-21
 Identities = 53/103 (51%), Positives = 64/103 (62%), Gaps = 1/103 (0%)
 Frame = -1

Query: 306 KKPKISDASTSNKNKEASAKSEVKAEDEEDHVPIAQRMKKPTFADKKSSSAKK-VTKHXX 130
           KKPK+SDAS S K K+ + K+E K ED++D VP AQRMKKP  +D KS S KK +TK   
Sbjct: 157 KKPKLSDASVSAKVKQVTVKAEPKKEDDDDDVPTAQRMKKPAASDSKSQSTKKTITKVVS 216

Query: 129 XXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
                             YS+S+K+ PSS EGQKWTTL HNGV
Sbjct: 217 SSMKKTSKKSKKVMKNSKYSKSTKLQPSSDEGQKWTTLEHNGV 259


>ref|XP_010266803.1| PREDICTED: DNA topoisomerase 1 alpha-like [Nelumbo nucifera]
          Length = 890

 Score = 93.6 bits (231), Expect(2) = 4e-20
 Identities = 52/105 (49%), Positives = 63/105 (60%)
 Frame = -1

Query: 315 SYKKKPKISDASTSNKNKEASAKSEVKAEDEEDHVPIAQRMKKPTFADKKSSSAKKVTKH 136
           S  KK K+S+ S   K K+ S KSE K EDE+D +PI+QR+KK   +D KS S KK TK 
Sbjct: 253 SLVKKLKVSEVSVPTKVKQTSIKSEPKEEDEDD-IPISQRLKKSASSDDKSLSLKKTTKV 311

Query: 135 XXXXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
                               YS+S+K+PPSSGEGQKWTTL HNGV
Sbjct: 312 VSSSFKKTTNKLKKAAKNSKYSKSTKMPPSSGEGQKWTTLEHNGV 356



 Score = 32.0 bits (71), Expect(2) = 4e-20
 Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 3/45 (6%)
 Frame = -2

Query: 428 KKSFLVKADQNGSASIERTPSSI---LKKRPADGVASAGQSHIKK 303
           +K  + K  QNGS+  +  P      L KRP  G  S+GQS +KK
Sbjct: 213 EKPLVSKLQQNGSSKRDNEPLKSPKNLNKRPLSGANSSGQSLVKK 257


>ref|XP_008245865.1| PREDICTED: DNA topoisomerase 1-like, partial [Prunus mume]
          Length = 724

 Score = 95.9 bits (237), Expect = 5e-20
 Identities = 51/106 (48%), Positives = 67/106 (63%), Gaps = 1/106 (0%)
 Frame = -1

Query: 315 SYKKKPKISDASTSNKNKEASAKSEVKAEDEEDHVPIAQRMKKPTFADKKSSSAKKVTKH 136
           S  K+PK+SD+STS KN + SAK E K ED++D +PI+QR+KKPT  +K   + +KVTK 
Sbjct: 85  SSAKRPKVSDSSTSIKNMQVSAKKEPKEEDDDDFIPISQRIKKPTSDNKSPVTKQKVTKV 144

Query: 135 XXXXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQ-KWTTLVHNGV 1
                               YS+S+ VPPSS +GQ KWT+LVHNGV
Sbjct: 145 GPSSIKKKIKKGQKASQFSKYSKSTTVPPSSNDGQKKWTSLVHNGV 190


>gb|PIA50017.1| hypothetical protein AQUCO_01300624v1 [Aquilegia coerulea]
          Length = 885

 Score = 95.9 bits (237), Expect = 5e-20
 Identities = 55/103 (53%), Positives = 61/103 (59%), Gaps = 1/103 (0%)
 Frame = -1

Query: 306 KKPKISDASTSNKNKEASAKSEVKAEDEEDHVPIAQRMKKPTFADKKSSSAKKV-TKHXX 130
           KK K SDAS SNK K  S K+E K ED++D VPIA RM K   +D KS   KK  +K   
Sbjct: 249 KKAKPSDASISNKVKHTSVKAEPKKEDDDDDVPIAHRMTKCNASDSKSQPIKKTPSKVVS 308

Query: 129 XXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
                             YSQS+KVPPSSGEGQKWTTLVHNGV
Sbjct: 309 SSLKKTNINSKKIVKKSKYSQSTKVPPSSGEGQKWTTLVHNGV 351


>ref|XP_018824786.1| PREDICTED: DNA topoisomerase 1-like [Juglans regia]
          Length = 898

 Score = 90.1 bits (222), Expect(2) = 9e-20
 Identities = 49/104 (47%), Positives = 66/104 (63%), Gaps = 2/104 (1%)
 Frame = -1

Query: 306 KKPKISDASTSNKNKEASAKSEVKAEDEEDHVPIAQRMKKPTFADKKSSSAK-KVTKHXX 130
           KK K+SD ST+ K K+ S K+E+K +D++DH+PIAQR+KKP+ +  KSSS K K  K   
Sbjct: 262 KKAKLSDTSTTVKIKQVSVKAELKTDDDDDHIPIAQRIKKPSASGNKSSSTKQKPVKVAS 321

Query: 129 XXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQ-KWTTLVHNGV 1
                             Y +S+K+ PS+G+GQ KWTTLVHNGV
Sbjct: 322 SSFKKTAQKSKKAVKNSKYIKSTKMSPSAGDGQKKWTTLVHNGV 365



 Score = 34.3 bits (77), Expect(2) = 9e-20
 Identities = 20/53 (37%), Positives = 29/53 (54%), Gaps = 2/53 (3%)
 Frame = -2

Query: 452 SRSSHSEGKKSFLVKADQNGSASIERTPSS--ILKKRPADGVASAGQSHIKKS 300
           ++S  SE KK    K  QNGS   ++   S  ++ KRP D   S+ QS +KK+
Sbjct: 212 NKSYESEEKKPLASKIQQNGSTLRDKQHKSSMVIGKRPLDTANSSDQSSVKKA 264


>ref|XP_016480589.1| PREDICTED: DNA topoisomerase 1-like isoform X1 [Nicotiana tabacum]
          Length = 879

 Score = 93.6 bits (231), Expect(2) = 2e-19
 Identities = 54/104 (51%), Positives = 65/104 (62%), Gaps = 2/104 (1%)
 Frame = -1

Query: 306 KKPKISDASTSNKNKEASAKSEVKAEDEEDH--VPIAQRMKKPTFADKKSSSAKKVTKHX 133
           KKP++SDAST   +K+AS K E K ED++D   VPI+QR+KK   +  K SSAKK TK  
Sbjct: 247 KKPRLSDASTPVSSKQASVKPEKKTEDDDDDDDVPISQRIKKSVASASKLSSAKKATK-- 304

Query: 132 XXXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
                              YS+SSK+ PSSGEGQKWTTLVHNGV
Sbjct: 305 ---VVSSSMKTKKKLKKSKYSKSSKLQPSSGEGQKWTTLVHNGV 345



 Score = 30.0 bits (66), Expect(2) = 2e-19
 Identities = 21/50 (42%), Positives = 28/50 (56%)
 Frame = -2

Query: 452 SRSSHSEGKKSFLVKADQNGSASIERTPSSILKKRPADGVASAGQSHIKK 303
           S+SS+SE  K    K  QNG  S     +++L KRP   V SA Q+ +KK
Sbjct: 207 SKSSNSEEVKP---KIRQNGLPS-----TTVLSKRPPGDVKSAAQASVKK 248


>ref|XP_016480590.1| PREDICTED: DNA topoisomerase 1-like isoform X2 [Nicotiana tabacum]
          Length = 803

 Score = 93.6 bits (231), Expect(2) = 2e-19
 Identities = 54/104 (51%), Positives = 65/104 (62%), Gaps = 2/104 (1%)
 Frame = -1

Query: 306 KKPKISDASTSNKNKEASAKSEVKAEDEEDH--VPIAQRMKKPTFADKKSSSAKKVTKHX 133
           KKP++SDAST   +K+AS K E K ED++D   VPI+QR+KK   +  K SSAKK TK  
Sbjct: 247 KKPRLSDASTPVSSKQASVKPEKKTEDDDDDDDVPISQRIKKSVASASKLSSAKKATK-- 304

Query: 132 XXXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
                              YS+SSK+ PSSGEGQKWTTLVHNGV
Sbjct: 305 ---VVSSSMKTKKKLKKSKYSKSSKLQPSSGEGQKWTTLVHNGV 345



 Score = 30.0 bits (66), Expect(2) = 2e-19
 Identities = 21/50 (42%), Positives = 28/50 (56%)
 Frame = -2

Query: 452 SRSSHSEGKKSFLVKADQNGSASIERTPSSILKKRPADGVASAGQSHIKK 303
           S+SS+SE  K    K  QNG  S     +++L KRP   V SA Q+ +KK
Sbjct: 207 SKSSNSEEVKP---KIRQNGLPS-----TTVLSKRPPGDVKSAAQASVKK 248


>dbj|BAG31377.1| topoisomerase I [Catharanthus roseus]
          Length = 910

 Score = 94.0 bits (232), Expect = 2e-19
 Identities = 50/103 (48%), Positives = 62/103 (60%), Gaps = 1/103 (0%)
 Frame = -1

Query: 306 KKPKISDASTSNKNKEASAKSEVKAEDEED-HVPIAQRMKKPTFADKKSSSAKKVTKHXX 130
           KKPK+S  +     K+AS K E KAED++D H+PI+QR+KK   ++ KSSS KK TK   
Sbjct: 272 KKPKLSSVTAPTNKKQASVKPEPKAEDDDDDHLPISQRIKKSPTSESKSSSIKKTTKVVS 331

Query: 129 XXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQKWTTLVHNGV 1
                             YS+SSKVPPSSGEG+KW  L HNGV
Sbjct: 332 SSVKKVNKKSKKVMKNSKYSKSSKVPPSSGEGKKWNKLEHNGV 374


>ref|XP_021812936.1| DNA topoisomerase 1 beta-like [Prunus avium]
          Length = 790

 Score = 92.8 bits (229), Expect = 5e-19
 Identities = 50/106 (47%), Positives = 66/106 (62%), Gaps = 1/106 (0%)
 Frame = -1

Query: 315 SYKKKPKISDASTSNKNKEASAKSEVKAEDEEDHVPIAQRMKKPTFADKKSSSAKKVTKH 136
           S  K+PK+SD+STS KN + SAK E K ED++D +PI+QR+KK T  +K   + +KVTK 
Sbjct: 151 SSAKRPKVSDSSTSIKNMQVSAKKEPKEEDDDDFIPISQRIKKATSDNKSPVTKQKVTKV 210

Query: 135 XXXXXXXXXXXXXXXXXXXMYSQSSKVPPSSGEGQ-KWTTLVHNGV 1
                               YS+S+ VPPSS +GQ KWT+LVHNGV
Sbjct: 211 GPSLIKKKIKKGQKASQFSKYSKSTTVPPSSNDGQKKWTSLVHNGV 256