BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia31_contig00028947
         (675 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011089959.1| SHUGOSHIN 2 isoform X2 [Sesamum indicum]          156   3e-73
ref|XP_011089958.1| SHUGOSHIN 2 isoform X1 [Sesamum indicum]          150   3e-71
ref|XP_022730606.1| SHUGOSHIN 2-like [Durio zibethinus]               136   1e-53
ref|XP_007016873.1| PREDICTED: shugoshin-1 [Theobroma cacao] >gi...   135   1e-53
ref|XP_022753883.1| SHUGOSHIN 2-like [Durio zibethinus]               130   6e-51
ref|XP_011032313.1| PREDICTED: shugoshin-1 isoform X1 [Populus e...   113   3e-49
ref|XP_009587803.1| PREDICTED: shugoshin-1 [Nicotiana tomentosif...   122   3e-48
gb|PIN02417.1| hypothetical protein CDL12_25069 [Handroanthus im...   165   3e-48
ref|XP_009790331.1| PREDICTED: coiled-coil domain-containing pro...   121   4e-48
ref|XP_003632272.1| PREDICTED: shugoshin-1 isoform X1 [Vitis vin...   122   1e-46
emb|CBI17144.3| unnamed protein product, partial [Vitis vinifera]     122   1e-46
gb|PIM98414.1| hypothetical protein CDL12_29107 [Handroanthus im...   165   2e-46
gb|OWM68925.1| hypothetical protein CDL15_Pgr025112 [Punica gran...   113   4e-46
ref|XP_021627516.1| SHUGOSHIN 2 [Manihot esculenta] >gi|10359084...   114   5e-46
ref|XP_023881852.1| shugoshin-1-like [Quercus suber] >gi|1336338...   119   1e-45
ref|XP_024031477.1| SHUGOSHIN 1 [Morus notabilis]                     125   1e-45
ref|XP_024035032.1| SHUGOSHIN 2 isoform X2 [Citrus clementina]        106   2e-45
ref|XP_021812126.1| shugoshin-1 isoform X2 [Prunus avium]             112   6e-45
gb|PPS00050.1| hypothetical protein GOBAR_AA20609 [Gossypium bar...   130   2e-44
ref|XP_016749851.1| PREDICTED: shugoshin-1-like [Gossypium hirsu...   130   2e-44

>ref|XP_011089959.1| SHUGOSHIN 2 isoform X2 [Sesamum indicum]
          Length = 289

 Score =  156 bits (395), Expect(2) = 3e-73
 Identities = 76/94 (80%), Positives = 84/94 (89%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           KE MALVKLIQDKNKIIEL+G+EIQNLRTCLQKMQLQNWNLAQ+N+HMLAELN+GKERLK
Sbjct: 58  KENMALVKLIQDKNKIIELTGIEIQNLRTCLQKMQLQNWNLAQANTHMLAELNLGKERLK 117

Query: 494 ALHHEVACKEALLKTKKSQVKGKAEANVQTTEFQ 393
            L HEV CKEALLKTK  Q+KG+ E N+Q TE Q
Sbjct: 118 GLQHEVMCKEALLKTKNLQLKGQGEVNLQKTELQ 151



 Score =  147 bits (371), Expect(2) = 3e-73
 Identities = 84/121 (69%), Positives = 91/121 (75%), Gaps = 1/121 (0%)
 Frame = -1

Query: 393 ALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRH-EEPTDNLFEIDDAMFPVVLPSNTP 217
           +LGSS I  QHAEKEA+V+KRRCLRRQSASSRIR  EEP DNLFEIDDA FPVV PS   
Sbjct: 183 SLGSSVIPPQHAEKEAIVNKRRCLRRQSASSRIRQPEEPADNLFEIDDAKFPVVSPS--- 239

Query: 216 THEAGPEFLTSSAGGVLTDGISVSKLEAEARRTSIGRPSRRAAEKVQSYKERPINVKMRR 37
                       A G+L D   VS+LEA+ARRTSI RPSR+A EKVQSYKERPINVKMRR
Sbjct: 240 ------------AEGLLADCSCVSELEAQARRTSISRPSRKAVEKVQSYKERPINVKMRR 287

Query: 36  S 34
           S
Sbjct: 288 S 288


>ref|XP_011089958.1| SHUGOSHIN 2 isoform X1 [Sesamum indicum]
          Length = 295

 Score =  150 bits (378), Expect(2) = 3e-71
 Identities = 76/100 (76%), Positives = 84/100 (84%), Gaps = 6/100 (6%)
 Frame = -2

Query: 674 KEKMALVKLIQDKN------KIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNM 513
           KE MALVKLIQDKN      KIIEL+G+EIQNLRTCLQKMQLQNWNLAQ+N+HMLAELN+
Sbjct: 58  KENMALVKLIQDKNLNFVNSKIIELTGIEIQNLRTCLQKMQLQNWNLAQANTHMLAELNL 117

Query: 512 GKERLKALHHEVACKEALLKTKKSQVKGKAEANVQTTEFQ 393
           GKERLK L HEV CKEALLKTK  Q+KG+ E N+Q TE Q
Sbjct: 118 GKERLKGLQHEVMCKEALLKTKNLQLKGQGEVNLQKTELQ 157



 Score =  147 bits (371), Expect(2) = 3e-71
 Identities = 84/121 (69%), Positives = 91/121 (75%), Gaps = 1/121 (0%)
 Frame = -1

Query: 393 ALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRH-EEPTDNLFEIDDAMFPVVLPSNTP 217
           +LGSS I  QHAEKEA+V+KRRCLRRQSASSRIR  EEP DNLFEIDDA FPVV PS   
Sbjct: 189 SLGSSVIPPQHAEKEAIVNKRRCLRRQSASSRIRQPEEPADNLFEIDDAKFPVVSPS--- 245

Query: 216 THEAGPEFLTSSAGGVLTDGISVSKLEAEARRTSIGRPSRRAAEKVQSYKERPINVKMRR 37
                       A G+L D   VS+LEA+ARRTSI RPSR+A EKVQSYKERPINVKMRR
Sbjct: 246 ------------AEGLLADCSCVSELEAQARRTSISRPSRKAVEKVQSYKERPINVKMRR 293

Query: 36  S 34
           S
Sbjct: 294 S 294


>ref|XP_022730606.1| SHUGOSHIN 2-like [Durio zibethinus]
          Length = 296

 Score =  136 bits (342), Expect(2) = 1e-53
 Identities = 64/91 (70%), Positives = 78/91 (85%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           KEKM L+KLI+++NKIIELSG E+QNLR CLQK+QLQNWNLAQSNS MLAELN+G++R+K
Sbjct: 55  KEKMTLMKLIEERNKIIELSGTELQNLRICLQKLQLQNWNLAQSNSQMLAELNLGRDRVK 114

Query: 494 ALHHEVACKEALLKTKKSQVKGKAEANVQTT 402
           AL HE+ CK+ALLK K  + KGKA+ N Q T
Sbjct: 115 ALQHELVCKDALLKAKNLEKKGKADINCQNT 145



 Score =  102 bits (255), Expect(2) = 1e-53
 Identities = 59/133 (44%), Positives = 76/133 (57%), Gaps = 3/133 (2%)
 Frame = -1

Query: 426 SRSERSDN*ISALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPTDNLFEIDDAM 247
           +R+ R +    ++G S+ S +  +K+   SKRRCLRRQSA  + +  EPT+NLFEIDD  
Sbjct: 172 TRNRRRNARSQSMGPSTTSQRGGDKQKNESKRRCLRRQSARFKSQEREPTENLFEIDDVT 231

Query: 246 FPVVLPSNTPTHEAGPEFLTSSAGGVLTDGISVSKLEAEA---RRTSIGRPSRRAAEKVQ 76
           F      +TP HE G                    L AE    +R+SIGRP R+A EKVQ
Sbjct: 232 FTATQQLDTPMHEDGSSITME----------ETCNLRAETQILKRSSIGRPLRKAVEKVQ 281

Query: 75  SYKERPINVKMRR 37
           SYKE P+NVKMRR
Sbjct: 282 SYKEAPVNVKMRR 294


>ref|XP_007016873.1| PREDICTED: shugoshin-1 [Theobroma cacao]
 gb|EOY34492.1| Shugoshin C terminus, putative [Theobroma cacao]
          Length = 302

 Score =  135 bits (340), Expect(2) = 1e-53
 Identities = 63/91 (69%), Positives = 78/91 (85%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           KEKM L+KLI+++NKIIELSG E+QNLR CLQK+QLQNWNLAQSNS MLAELN+G++++K
Sbjct: 55  KEKMTLMKLIEERNKIIELSGTELQNLRNCLQKLQLQNWNLAQSNSQMLAELNLGRDKVK 114

Query: 494 ALHHEVACKEALLKTKKSQVKGKAEANVQTT 402
           AL HE+ CK+ALLK K  + KGKA+ N Q T
Sbjct: 115 ALQHELVCKDALLKAKNLEKKGKADINCQNT 145



 Score =  103 bits (256), Expect(2) = 1e-53
 Identities = 59/132 (44%), Positives = 80/132 (60%), Gaps = 2/132 (1%)
 Frame = -1

Query: 426 SRSERSDN*ISALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPTDNLFEIDDAM 247
           +R+ R      ++G S+ S + A+KE + SKRRCLRRQSA  + +  EPT NLFEI+D  
Sbjct: 172 NRNRRRSTRSQSMGPSTTSQRGADKEKIESKRRCLRRQSARFKSQEREPTKNLFEIEDVN 231

Query: 246 FPVVLPSNTPTHEAGPEFLTSSAGGVLTDGISVSKLEAE--ARRTSIGRPSRRAAEKVQS 73
           +      +TP HE  P   T S    +T   + + +  +   +R S GRP R+AAEKVQS
Sbjct: 232 YAAAQQLDTPMHEDDP---TPSLVSSITKEEACNPMTGKQILKRPSFGRPLRKAAEKVQS 288

Query: 72  YKERPINVKMRR 37
           YKE P+NVKMRR
Sbjct: 289 YKEVPLNVKMRR 300


>ref|XP_022753883.1| SHUGOSHIN 2-like [Durio zibethinus]
          Length = 294

 Score =  130 bits (328), Expect(2) = 6e-51
 Identities = 61/91 (67%), Positives = 77/91 (84%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           KEKM L++LI+++N+IIELSG E+QNLR CLQK+QLQNWNLAQSNS MLAELN+G++R+K
Sbjct: 55  KEKMTLMELIEERNRIIELSGTELQNLRICLQKLQLQNWNLAQSNSQMLAELNLGRDRVK 114

Query: 494 ALHHEVACKEALLKTKKSQVKGKAEANVQTT 402
           AL HE+ CK+ALLK K  + K KA+ N Q T
Sbjct: 115 ALQHELVCKDALLKAKNQEKKEKADINSQNT 145



 Score = 99.0 bits (245), Expect(2) = 6e-51
 Identities = 59/132 (44%), Positives = 84/132 (63%), Gaps = 2/132 (1%)
 Frame = -1

Query: 426 SRSERSDN*ISALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPT-DNLFEIDDA 250
           +R+ R +    ++G S+ S +  +KE + +KRRCLRRQSA  + +  EPT +NLFEID+ 
Sbjct: 169 TRNRRRNARSQSMGPSTTSQRDGDKEKIENKRRCLRRQSARFKSQESEPTAENLFEIDN- 227

Query: 249 MFPVVLPSNTPTHEAGPEFLTSSAGGVLTDGISVSKLEAEA-RRTSIGRPSRRAAEKVQS 73
               V  + TP HE G   L SS   ++T+     + E +  +R+SIGRP R+A EKVQS
Sbjct: 228 ----VKVAATPMHEGGSTLLGSS---IITEESCNPRAETQILKRSSIGRPLRKAVEKVQS 280

Query: 72  YKERPINVKMRR 37
           YKE P+NVK+RR
Sbjct: 281 YKEAPVNVKLRR 292


>ref|XP_011032313.1| PREDICTED: shugoshin-1 isoform X1 [Populus euphratica]
          Length = 301

 Score =  113 bits (282), Expect(2) = 3e-49
 Identities = 54/87 (62%), Positives = 71/87 (81%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           +EK  L+KL++++NKIIELSG ++++LR   QK+Q QNWNLAQSNS MLAELN+G+E+LK
Sbjct: 58  QEKEMLLKLVEERNKIIELSGNKLRDLRMNYQKLQAQNWNLAQSNSQMLAELNLGREKLK 117

Query: 494 ALHHEVACKEALLKTKKSQVKGKAEAN 414
           AL HE+ CKEALLK K    +GKA+ N
Sbjct: 118 ALQHEIVCKEALLKAKNLGPQGKADMN 144



 Score =  110 bits (276), Expect(2) = 3e-49
 Identities = 68/134 (50%), Positives = 85/134 (63%), Gaps = 3/134 (2%)
 Frame = -1

Query: 426 SRSERSDN*ISALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPTDNLFEIDDAM 247
           SRS R      ++G S+ + Q  EKE   +KRRC+RRQSA+SR +  EP +NLFEI+D  
Sbjct: 174 SRSRRRTARSRSMGPSTTNRQTVEKEKAETKRRCVRRQSAASRSQEREPAENLFEIEDVR 233

Query: 246 FPVVLPSNTPTHEAGPEFLTSSAGGVLTDGISVSKLEAEAR---RTSIGRPSRRAAEKVQ 76
           FPV   SN+         LTSS+         + KL  EA+   R+SIGRPSRRAAEKVQ
Sbjct: 234 FPV---SNSRDKSMKENGLTSSS----IPKEEICKLSTEAQVSHRSSIGRPSRRAAEKVQ 286

Query: 75  SYKERPINVKMRRS 34
           SYKE P+NVKMRR+
Sbjct: 287 SYKEVPLNVKMRRA 300


>ref|XP_009587803.1| PREDICTED: shugoshin-1 [Nicotiana tomentosiformis]
 ref|XP_016464918.1| PREDICTED: shugoshin-1-like [Nicotiana tabacum]
          Length = 297

 Score =  122 bits (307), Expect(2) = 3e-48
 Identities = 60/97 (61%), Positives = 79/97 (81%), Gaps = 4/97 (4%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           KE +ALVKL+QDKNKIIELSG+EIQ +R  LQKMQLQNWNLAQSNSHMLAELN+ +E++K
Sbjct: 57  KENVALVKLVQDKNKIIELSGIEIQKMRIHLQKMQLQNWNLAQSNSHMLAELNLNREKMK 116

Query: 494 ALHHEVACKEALLKTK----KSQVKGKAEANVQTTEF 396
           +L HE+ CKEALLK++    + + + + + ++Q  EF
Sbjct: 117 SLQHELVCKEALLKSRNLEEEQEQRNQLKNDLQDEEF 153



 Score = 98.2 bits (243), Expect(2) = 3e-48
 Identities = 58/122 (47%), Positives = 83/122 (68%), Gaps = 3/122 (2%)
 Frame = -1

Query: 393 ALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPT-DNLFEIDDAMFPVVLPSNTP 217
           ++G S+ S Q AEKEA  +KRRCLRR+S +S+++H E   ++LFE++     + +P N+P
Sbjct: 180 SMGHSTSSQQAAEKEAAENKRRCLRRKSTNSKVQHSEAAAEDLFELEG----LAVPFNSP 235

Query: 216 THEAGPEFLT-SSAGGVLTDGISVSKLEAE-ARRTSIGRPSRRAAEKVQSYKERPINVKM 43
            H+     L  SS   V+ D    S+  A+ +RR SIGRPSR+AAEK+QSYKE P+N+KM
Sbjct: 236 VHDYDLTPLPFSSIEEVIKDEKDSSENVAQLSRRASIGRPSRKAAEKIQSYKEIPVNIKM 295

Query: 42  RR 37
           RR
Sbjct: 296 RR 297


>gb|PIN02417.1| hypothetical protein CDL12_25069 [Handroanthus impetiginosus]
          Length = 163

 Score =  165 bits (417), Expect = 3e-48
 Identities = 90/140 (64%), Positives = 101/140 (72%), Gaps = 1/140 (0%)
 Frame = -1

Query: 450 KEVTSEGQSRSERSDN*ISALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPTDN 271
           K   + G+ R  RS     +LGSS+ISHQHAEK+A  SKRRCLRRQSA SRI HEEPT+N
Sbjct: 27  KSCYASGRRRHSRS----RSLGSSTISHQHAEKDAAASKRRCLRRQSAGSRITHEEPTEN 82

Query: 270 LFEIDDAMFPVVLPSNTPTHEAGPEFLTSSAGGVLT-DGISVSKLEAEARRTSIGRPSRR 94
           LFEIDD  FPVV PSN   +E G   LT    GVL+ DG   S+LE EARRTSI RP R+
Sbjct: 83  LFEIDDVNFPVVSPSNQTKNEVGSSLLTKPTEGVLSNDGSRFSELEREARRTSIARPMRK 142

Query: 93  AAEKVQSYKERPINVKMRRS 34
           A EKVQSYKE P+NVKMRRS
Sbjct: 143 AVEKVQSYKEIPLNVKMRRS 162


>ref|XP_009790331.1| PREDICTED: coiled-coil domain-containing protein 18 [Nicotiana
           sylvestris]
 ref|XP_016461573.1| PREDICTED: uncharacterized protein LOC107784894 [Nicotiana tabacum]
          Length = 298

 Score =  121 bits (304), Expect(2) = 4e-48
 Identities = 57/76 (75%), Positives = 69/76 (90%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           KE +ALVKL+QDKNKIIELSG+EIQ +R  LQKMQLQNWNLAQSNSHMLAELN+ +E++K
Sbjct: 57  KENVALVKLVQDKNKIIELSGIEIQKMRIHLQKMQLQNWNLAQSNSHMLAELNLNREKMK 116

Query: 494 ALHHEVACKEALLKTK 447
           +L HE+ CKEALLK++
Sbjct: 117 SLQHELVCKEALLKSR 132



 Score = 98.6 bits (244), Expect(2) = 4e-48
 Identities = 62/142 (43%), Positives = 90/142 (63%), Gaps = 3/142 (2%)
 Frame = -1

Query: 453 NKEVTSEGQSRSERSDN*ISALGSSSISHQHAEKEAVVSKRRCLRRQSAS-SRIRHEEPT 277
           NK    +  +R +R+     ++G ++ S Q AEKEA  +KRRCLRR+S + S+I+  EP 
Sbjct: 162 NKHSKPKNGNRRQRATR-SQSMGHTTSSQQGAEKEAAENKRRCLRRKSTNNSKIQQSEPA 220

Query: 276 -DNLFEIDDAMFPVVLPSNTPTHEAGPEFLTSSAGGVLTDGISVSKLEAE-ARRTSIGRP 103
            ++LFE++     + +P N+P HE       SS   V+ D    S+  A+ +RR S+GRP
Sbjct: 221 AEDLFELEG----LAVPFNSPVHEDDLPLPYSSIEEVMKDEKDSSENVAQLSRRASVGRP 276

Query: 102 SRRAAEKVQSYKERPINVKMRR 37
           SR+AAEKVQSYKE P+N+KMRR
Sbjct: 277 SRKAAEKVQSYKEIPVNIKMRR 298


>ref|XP_003632272.1| PREDICTED: shugoshin-1 isoform X1 [Vitis vinifera]
          Length = 297

 Score =  122 bits (306), Expect(2) = 1e-46
 Identities = 59/94 (62%), Positives = 75/94 (79%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           KE  AL+KL+ ++ KIIELSGVE+Q LRT  QK+QLQNWNLAQSNS MLAELN+G+E++K
Sbjct: 57  KENAALMKLLAERQKIIELSGVELQKLRTNYQKLQLQNWNLAQSNSQMLAELNLGREKMK 116

Query: 494 ALHHEVACKEALLKTKKSQVKGKAEANVQTTEFQ 393
           A+ HE+ CKEALLK    +++GKA+ N Q T  Q
Sbjct: 117 AVQHELICKEALLKANNLELEGKAKMNCQKTGIQ 150



 Score = 92.8 bits (229), Expect(2) = 1e-46
 Identities = 60/127 (47%), Positives = 74/127 (58%), Gaps = 8/127 (6%)
 Frame = -1

Query: 393 ALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPTDNLFEIDDAMFPV------VL 232
           ++GSS+   Q  EKE V +KR C RRQS   + +  EP  +LFEI+DA  PV      + 
Sbjct: 182 SMGSSTAYQQVEEKETVETKRHCSRRQSCRFKSQQREPNGDLFEIEDAKLPVGWHEGGLA 241

Query: 231 PSNTPTH-EAGPEFLTSSAGGVLTDGISVSKLEAE-ARRTSIGRPSRRAAEKVQSYKERP 58
           PSN+P   E G E               V K EA  ++R+SIGRP RRAAEKVQSYKE P
Sbjct: 242 PSNSPIKKEEGDESC-------------VEKHEARGSQRSSIGRPLRRAAEKVQSYKEAP 288

Query: 57  INVKMRR 37
           +N KMRR
Sbjct: 289 LNTKMRR 295


>emb|CBI17144.3| unnamed protein product, partial [Vitis vinifera]
          Length = 292

 Score =  122 bits (306), Expect(2) = 1e-46
 Identities = 59/94 (62%), Positives = 75/94 (79%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           KE  AL+KL+ ++ KIIELSGVE+Q LRT  QK+QLQNWNLAQSNS MLAELN+G+E++K
Sbjct: 52  KENAALMKLLAERQKIIELSGVELQKLRTNYQKLQLQNWNLAQSNSQMLAELNLGREKMK 111

Query: 494 ALHHEVACKEALLKTKKSQVKGKAEANVQTTEFQ 393
           A+ HE+ CKEALLK    +++GKA+ N Q T  Q
Sbjct: 112 AVQHELICKEALLKANNLELEGKAKMNCQKTGIQ 145



 Score = 92.8 bits (229), Expect(2) = 1e-46
 Identities = 60/127 (47%), Positives = 74/127 (58%), Gaps = 8/127 (6%)
 Frame = -1

Query: 393 ALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPTDNLFEIDDAMFPV------VL 232
           ++GSS+   Q  EKE V +KR C RRQS   + +  EP  +LFEI+DA  PV      + 
Sbjct: 177 SMGSSTAYQQVEEKETVETKRHCSRRQSCRFKSQQREPNGDLFEIEDAKLPVGWHEGGLA 236

Query: 231 PSNTPTH-EAGPEFLTSSAGGVLTDGISVSKLEAE-ARRTSIGRPSRRAAEKVQSYKERP 58
           PSN+P   E G E               V K EA  ++R+SIGRP RRAAEKVQSYKE P
Sbjct: 237 PSNSPIKKEEGDESC-------------VEKHEARGSQRSSIGRPLRRAAEKVQSYKEAP 283

Query: 57  INVKMRR 37
           +N KMRR
Sbjct: 284 LNTKMRR 290


>gb|PIM98414.1| hypothetical protein CDL12_29107 [Handroanthus impetiginosus]
          Length = 304

 Score =  165 bits (417), Expect = 2e-46
 Identities = 90/140 (64%), Positives = 101/140 (72%), Gaps = 1/140 (0%)
 Frame = -1

Query: 450 KEVTSEGQSRSERSDN*ISALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPTDN 271
           K   + G+ R  RS     +LGSS+ISHQHAEK+A  SKRRCLRRQSA SRI HEEPT+N
Sbjct: 168 KSCYASGRRRHSRS----RSLGSSTISHQHAEKDAAASKRRCLRRQSAGSRITHEEPTEN 223

Query: 270 LFEIDDAMFPVVLPSNTPTHEAGPEFLTSSAGGVLT-DGISVSKLEAEARRTSIGRPSRR 94
           LFEIDD  FPVV PSN   +E G   LT    GVL+ DG   S+LE EARRTSI RP R+
Sbjct: 224 LFEIDDVNFPVVSPSNQTKNEVGSSLLTKPTEGVLSNDGSRFSELEREARRTSIARPMRK 283

Query: 93  AAEKVQSYKERPINVKMRRS 34
           A EKVQSYKE P+NVKMRRS
Sbjct: 284 AVEKVQSYKEIPLNVKMRRS 303



 Score =  156 bits (394), Expect = 5e-43
 Identities = 80/95 (84%), Positives = 85/95 (89%), Gaps = 1/95 (1%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           KEKMALVKLIQDKNKIIELSG+EIQNLR CLQKMQLQNWNLAQSNSHM AELN+GKERLK
Sbjct: 58  KEKMALVKLIQDKNKIIELSGIEIQNLRNCLQKMQLQNWNLAQSNSHMSAELNLGKERLK 117

Query: 494 ALHHEVACKEALLKTKKSQV-KGKAEANVQTTEFQ 393
           AL HEVACKEALLKTK  Q+ KG+ E NVQ T+ Q
Sbjct: 118 ALQHEVACKEALLKTKNLQLKKGQEEVNVQKTDLQ 152


>gb|OWM68925.1| hypothetical protein CDL15_Pgr025112 [Punica granatum]
          Length = 304

 Score =  113 bits (282), Expect(2) = 4e-46
 Identities = 57/89 (64%), Positives = 70/89 (78%), Gaps = 1/89 (1%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           +E  ALVKL++++NKIIELSG E+Q LR   QK QLQNWNLAQ+NS MLAELN+ K++LK
Sbjct: 58  QENGALVKLVEERNKIIELSGAELQRLRISYQKTQLQNWNLAQTNSQMLAELNLAKDKLK 117

Query: 494 ALHHEVACKEALLKTKKSQV-KGKAEANV 411
           A  HE+ACKEALLK KK +V +   E NV
Sbjct: 118 AFQHELACKEALLKAKKLEVERNMVEKNV 146



 Score =  100 bits (249), Expect(2) = 4e-46
 Identities = 61/133 (45%), Positives = 78/133 (58%)
 Frame = -1

Query: 435 EGQSRSERSDN*ISALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPTDNLFEID 256
           +  SR+ R      ++G  S + Q  EKE    KRRCLRRQSA    +  EP +NLFEI+
Sbjct: 176 QAPSRNRRHHQRSQSMGVPSTNKQDLEKEKAEKKRRCLRRQSARFMSQEREPAENLFEIE 235

Query: 255 DAMFPVVLPSNTPTHEAGPEFLTSSAGGVLTDGISVSKLEAEARRTSIGRPSRRAAEKVQ 76
           +A FP+     T   EAGP     S+     +  + S  + E RR+S+ RP RRAAEKVQ
Sbjct: 236 EAKFPI-----TTHPEAGPSSSDQSSMKEDKENDASSGAD-EDRRSSVARPLRRAAEKVQ 289

Query: 75  SYKERPINVKMRR 37
           SYKE P+NVKMRR
Sbjct: 290 SYKEPPLNVKMRR 302


>ref|XP_021627516.1| SHUGOSHIN 2 [Manihot esculenta]
 gb|OAY38089.1| hypothetical protein MANES_11G151800 [Manihot esculenta]
          Length = 298

 Score =  114 bits (285), Expect(2) = 5e-46
 Identities = 57/94 (60%), Positives = 73/94 (77%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           KEK  L++LI++++KII LS  +++NLR   QK+QLQNWNLAQSNS MLAELN+G+E+LK
Sbjct: 55  KEKATLMQLIEERDKIIALSDNQLKNLRMHYQKLQLQNWNLAQSNSQMLAELNLGREKLK 114

Query: 494 ALHHEVACKEALLKTKKSQVKGKAEANVQTTEFQ 393
           AL HEV CK+ALLK K  + +GKAE   Q T  Q
Sbjct: 115 ALQHEVVCKDALLKAKSLEQEGKAEIKNQNTGSQ 148



 Score = 99.0 bits (245), Expect(2) = 5e-46
 Identities = 56/120 (46%), Positives = 74/120 (61%), Gaps = 1/120 (0%)
 Frame = -1

Query: 393 ALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPTDNLFEIDDAMFPVVLP-SNTP 217
           ++G S+ S +  EKE + +KRRCLRRQSA  + +  EP +NLFEI+ A FP+  P   TP
Sbjct: 181 SMGPSTTSKEGLEKERLENKRRCLRRQSARFKSQEREPMENLFEIEVAKFPIAQPLDTTP 240

Query: 216 THEAGPEFLTSSAGGVLTDGISVSKLEAEARRTSIGRPSRRAAEKVQSYKERPINVKMRR 37
             E G     ++A  +  +          A R+S+GRP RRAAEKVQSYKE P+N KMRR
Sbjct: 241 MQEYG----LTTASVIKEETFEPGNEPHVAHRSSLGRPVRRAAEKVQSYKEVPLNTKMRR 296


>ref|XP_023881852.1| shugoshin-1-like [Quercus suber]
 gb|POE73798.1| shugoshin 2 [Quercus suber]
          Length = 299

 Score =  119 bits (297), Expect(2) = 1e-45
 Identities = 55/85 (64%), Positives = 69/85 (81%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           KE+M LVKLI ++NKIIELSG E+Q +R  LQK+Q+QNWNLAQSNS M AELN+GK++LK
Sbjct: 54  KERMTLVKLIAERNKIIELSGAELQKMRVSLQKLQMQNWNLAQSNSQMFAELNLGKDKLK 113

Query: 494 ALHHEVACKEALLKTKKSQVKGKAE 420
            L HE+ CK ALLK K  +++GK E
Sbjct: 114 ELRHELGCKNALLKAKNMELEGKTE 138



 Score = 93.2 bits (230), Expect(2) = 1e-45
 Identities = 60/130 (46%), Positives = 78/130 (60%), Gaps = 1/130 (0%)
 Frame = -1

Query: 423 RSERSDN*ISALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPTDNLFEIDDAMF 244
           R+ R      ++G S+   +  +KE V +KRRCLRRQSA  +    EPT+NLFEI+DA F
Sbjct: 169 RNRRRTARSQSMGPSTSHQKVVDKEKVENKRRCLRRQSARFKSHEREPTENLFEIEDAKF 228

Query: 243 PVVLPSNTPTHEAGPEFLTSSAGGVLTDGISVSKLEAEAR-RTSIGRPSRRAAEKVQSYK 67
            +V  S+  +    P  L SS          V + EA+A  R+SIGRP R+AAEKVQSYK
Sbjct: 229 -LVSQSHENSIREEPVPLNSSFKNEEEGENCVPRNEAQASLRSSIGRPLRKAAEKVQSYK 287

Query: 66  ERPINVKMRR 37
           E P+  KMRR
Sbjct: 288 EAPLKSKMRR 297


>ref|XP_024031477.1| SHUGOSHIN 1 [Morus notabilis]
          Length = 279

 Score =  125 bits (315), Expect(2) = 1e-45
 Identities = 61/91 (67%), Positives = 75/91 (82%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           KE+M+ +KLI ++NKIIELSG E+QNLR  LQK+QLQNWNLAQSNS MLAELN+G+ER+K
Sbjct: 49  KERMSWMKLIAERNKIIELSGAELQNLRVSLQKLQLQNWNLAQSNSQMLAELNLGRERIK 108

Query: 494 ALHHEVACKEALLKTKKSQVKGKAEANVQTT 402
           AL HE+ CK+ALLK K    +GK +AN Q T
Sbjct: 109 ALQHELRCKDALLKAKNLCQEGKTKANCQKT 139



 Score = 85.9 bits (211), Expect(2) = 1e-45
 Identities = 51/119 (42%), Positives = 68/119 (57%)
 Frame = -1

Query: 393 ALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPTDNLFEIDDAMFPVVLPSNTPT 214
           ++G S+ S +H +KE V +KRRC+RRQS   +    +PT+NLFEI+DA F    P  T  
Sbjct: 171 SMGPSTTSQKHDDKE-VENKRRCVRRQSVQLKSHERKPTENLFEIEDAKFD--SPKQTGK 227

Query: 213 HEAGPEFLTSSAGGVLTDGISVSKLEAEARRTSIGRPSRRAAEKVQSYKERPINVKMRR 37
                  + +S  GV T            RR+S+GRP R+A  KVQSYKE P+  KMRR
Sbjct: 228 ENREESCIPNSEAGVTT------------RRSSLGRPMRKAVVKVQSYKEVPLKFKMRR 274


>ref|XP_024035032.1| SHUGOSHIN 2 isoform X2 [Citrus clementina]
          Length = 287

 Score =  106 bits (264), Expect(2) = 2e-45
 Identities = 53/102 (51%), Positives = 71/102 (69%), Gaps = 10/102 (9%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           KEK  L++ I+++NKIIELS  E+ NLR  +QK+QLQNWNLAQSNS  LAE+N+G+E++K
Sbjct: 56  KEKTTLMRFIEERNKIIELSSSELHNLRISIQKLQLQNWNLAQSNSQYLAEINLGREKVK 115

Query: 494 ALHHEVACKEALLKTKK----------SQVKGKAEANVQTTE 399
           AL HE+ CK+AL+K K            +   KA  NV+T E
Sbjct: 116 ALQHELVCKDALIKAKSIVKEEGEKVIEECVPKANENVKTCE 157



 Score =  105 bits (261), Expect(2) = 2e-45
 Identities = 58/131 (44%), Positives = 82/131 (62%), Gaps = 1/131 (0%)
 Frame = -1

Query: 423 RSERSDN*ISALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPTDNLFEIDDAMF 244
           R+ R      ++G S+   + AEKE V +KRRC+RRQSA  + +   PT+NLFEI+D+  
Sbjct: 158 RNRRRSTRCKSMGPSTTRQKVAEKENVENKRRCVRRQSARFKSQERAPTENLFEIEDSKL 217

Query: 243 PVVLPSNTPTHEAGPEFLTSSAGGVLTDGISVSKLEAE-ARRTSIGRPSRRAAEKVQSYK 67
           P   P + P HE   +    +      +  S S+ EA  ++R+S+GRPSR+AAEKVQSYK
Sbjct: 218 PATQPLDDPMHE---DNSIQAGSSTANEEFSSSRNEARLSQRSSMGRPSRKAAEKVQSYK 274

Query: 66  ERPINVKMRRS 34
           E P+ VKMRR+
Sbjct: 275 ELPLKVKMRRT 285


>ref|XP_021812126.1| shugoshin-1 isoform X2 [Prunus avium]
          Length = 281

 Score =  112 bits (280), Expect(2) = 6e-45
 Identities = 55/95 (57%), Positives = 74/95 (77%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           +E+M L++L+ ++NKI+ELSG E+Q LR  LQK+Q QN NLAQSNS MLAELN+G+E++K
Sbjct: 49  RERMTLMRLVAERNKIVELSGAELQKLRISLQKLQQQNLNLAQSNSRMLAELNLGREKVK 108

Query: 494 ALHHEVACKEALLKTKKSQVKGKAEANVQTTEFQL 390
            L HE+ CK+ALLK K  +++GKAE   Q T  QL
Sbjct: 109 TLQHELLCKDALLKAKNLEIEGKAE-KCQNTVSQL 142



 Score = 97.4 bits (241), Expect(2) = 6e-45
 Identities = 56/120 (46%), Positives = 71/120 (59%)
 Frame = -1

Query: 393 ALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPTDNLFEIDDAMFPVVLPSNTPT 214
           ++G S+   +   KE V +KRRCLRRQSA    R    T+NLFEI+D  FPV    + P 
Sbjct: 172 SMGPSTACPKVENKEKVENKRRCLRRQSA----RFRSQTENLFEIEDVKFPVSRTPDNPM 227

Query: 213 HEAGPEFLTSSAGGVLTDGISVSKLEAEARRTSIGRPSRRAAEKVQSYKERPINVKMRRS 34
           H +GP  L SS            K     RR+S+GRP R+AAEKV SYKE P+NVK+RR+
Sbjct: 228 HNSGPTPLISSTS-------KEEKENCAPRRSSVGRPPRKAAEKVHSYKEVPLNVKLRRA 280


>gb|PPS00050.1| hypothetical protein GOBAR_AA20609 [Gossypium barbadense]
          Length = 278

 Score =  130 bits (327), Expect(2) = 2e-44
 Identities = 60/92 (65%), Positives = 79/92 (85%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           KEKM L+++++++NKIIELSG E+QNLRT LQK+QLQNWNLAQSNS +LAELN+G++R+K
Sbjct: 55  KEKMTLIEVVEERNKIIELSGTELQNLRTSLQKLQLQNWNLAQSNSQILAELNLGRDRVK 114

Query: 494 ALHHEVACKEALLKTKKSQVKGKAEANVQTTE 399
           AL HE+ CK+ALLK K  + KGKA+ N Q T+
Sbjct: 115 ALQHELVCKDALLKAKNLERKGKADINDQNTD 146



 Score = 77.8 bits (190), Expect(2) = 2e-44
 Identities = 52/133 (39%), Positives = 76/133 (57%), Gaps = 2/133 (1%)
 Frame = -1

Query: 426 SRSERSDN*ISALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPTDNLFEIDDAM 247
           +R+ R +    ++G S+ S +  +KE   +KRRCLRR++A  R + +E  +NLFEIDD  
Sbjct: 157 TRNRRRNARSQSMGPSTTSRRSGDKEKAENKRRCLRRRTA--RFKSQE-AENLFEIDDLD 213

Query: 246 FPVVLPSNTPTHEAGPEFLTSSAGGVLTDGISVSKLEAEARRTSIGRPS--RRAAEKVQS 73
           +    P +TP HE     L+           + S   AE+R +   RP   R+A EKVQS
Sbjct: 214 YVGAQP-DTPMHELAASSLSMEE--------ACSNPRAESRSSIAARPLPLRKAVEKVQS 264

Query: 72  YKERPINVKMRRS 34
           YKE P+N+KMRR+
Sbjct: 265 YKEVPLNIKMRRN 277


>ref|XP_016749851.1| PREDICTED: shugoshin-1-like [Gossypium hirsutum]
 ref|XP_017644777.1| PREDICTED: shugoshin-1 [Gossypium arboreum]
 gb|KHG02349.1| Shugoshin-1 [Gossypium arboreum]
          Length = 278

 Score =  130 bits (327), Expect(2) = 2e-44
 Identities = 60/92 (65%), Positives = 79/92 (85%)
 Frame = -2

Query: 674 KEKMALVKLIQDKNKIIELSGVEIQNLRTCLQKMQLQNWNLAQSNSHMLAELNMGKERLK 495
           KEKM L+++++++NKIIELSG E+QNLRT LQK+QLQNWNLAQSNS +LAELN+G++R+K
Sbjct: 55  KEKMTLIEVVEERNKIIELSGTELQNLRTSLQKLQLQNWNLAQSNSQILAELNLGRDRVK 114

Query: 494 ALHHEVACKEALLKTKKSQVKGKAEANVQTTE 399
           AL HE+ CK+ALLK K  + KGKA+ N Q T+
Sbjct: 115 ALQHELVCKDALLKAKNLERKGKADINDQNTD 146



 Score = 77.8 bits (190), Expect(2) = 2e-44
 Identities = 52/133 (39%), Positives = 76/133 (57%), Gaps = 2/133 (1%)
 Frame = -1

Query: 426 SRSERSDN*ISALGSSSISHQHAEKEAVVSKRRCLRRQSASSRIRHEEPTDNLFEIDDAM 247
           +R+ R +    ++G S+ S +  +KE   +KRRCLRR++A  R + +E  +NLFEIDD  
Sbjct: 157 TRNRRRNARSQSMGPSTTSRRSGDKEKAENKRRCLRRRTA--RFKSQE-AENLFEIDDLD 213

Query: 246 FPVVLPSNTPTHEAGPEFLTSSAGGVLTDGISVSKLEAEARRTSIGRPS--RRAAEKVQS 73
           +    P +TP HE     L+           + S   AE+R +   RP   R+A EKVQS
Sbjct: 214 YVGAQP-DTPMHELAASSLSMEE--------ACSNPRAESRSSIAARPLPLRKAVEKVQS 264

Query: 72  YKERPINVKMRRS 34
           YKE P+N+KMRR+
Sbjct: 265 YKEVPLNIKMRRN 277