BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia31_contig00028526
         (945 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PIM97765.1| 1,4-alpha-glucan branching enzyme/starch branchin...   554   0.0  
ref|XP_011092269.1| isoamylase 2, chloroplastic [Sesamum indicum...   546   0.0  
gb|EYU33286.1| hypothetical protein MIMGU_mgv1a001869mg [Erythra...   539   0.0  
ref|XP_012842325.1| PREDICTED: isoamylase 2, chloroplastic [Eryt...   539   0.0  
ref|XP_022895653.1| isoamylase 2, chloroplastic isoform X2 [Olea...   518   e-176
ref|XP_022895652.1| isoamylase 2, chloroplastic isoform X1 [Olea...   518   e-176
gb|KZV17747.1| isoamylase isoform 2 [Dorcoceras hygrometricum]        518   e-176
ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] >gi...   473   e-158
ref|XP_006355876.1| PREDICTED: isoamylase isoform 2 isoform X1 [...   473   e-158
dbj|BAW33107.1| isoamylase [Ipomoea batatas]                          470   e-157
ref|XP_019161030.1| PREDICTED: isoamylase 2, chloroplastic [Ipom...   470   e-157
gb|AOQ26242.1| ISA2 [Actinidia deliciosa]                             466   e-155
ref|XP_004247144.2| PREDICTED: isoamylase 2, chloroplastic [Sola...   460   e-153
ref|XP_015087879.1| PREDICTED: isoamylase 2, chloroplastic [Sola...   458   e-152
ref|XP_016450982.1| PREDICTED: isoamylase 2, chloroplastic-like ...   456   e-151
ref|XP_019231687.1| PREDICTED: isoamylase 2, chloroplastic [Nico...   455   e-151
gb|AAY89379.1| isoamylase isoform 2, partial [Nicotiana langsdor...   444   e-151
gb|PHU26272.1| Isoamylase 2, chloroplastic [Capsicum chinense]        452   e-150
gb|PHT89964.1| Isoamylase 2, chloroplastic [Capsicum annuum]          450   e-149
ref|XP_016559778.1| PREDICTED: isoamylase 2, chloroplastic-like ...   450   e-149

>gb|PIM97765.1| 1,4-alpha-glucan branching enzyme/starch branching enzyme II
            [Handroanthus impetiginosus]
          Length = 853

 Score =  554 bits (1427), Expect = 0.0
 Identities = 261/315 (82%), Positives = 284/315 (90%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDFGLDLPRKFLGKLCEEPAF 180
            IWHA VDCS  FV YGYRCR+G +NKGH VLLDPYA+VIED GLDLP+++LGKL +EP F
Sbjct: 273  IWHASVDCSPHFVSYGYRCRSGPDNKGHNVLLDPYAKVIEDIGLDLPQRYLGKLFKEPPF 332

Query: 181  DWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVL 360
            DWSG+F PS+PME+LIIYRLNVTRFT   SSKLPGDIAG+F+G+SEKLHHFK+LGVNAVL
Sbjct: 333  DWSGEFCPSIPMEKLIIYRLNVTRFTRHNSSKLPGDIAGSFSGVSEKLHHFKDLGVNAVL 392

Query: 361  LEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFT 540
            LEPIFPFDE+K PYFP HFFSPG+LYGPSG+P +VAKSMKEMVKKLHASGIEVLLEVVFT
Sbjct: 393  LEPIFPFDEKKRPYFPAHFFSPGNLYGPSGNPNSVAKSMKEMVKKLHASGIEVLLEVVFT 452

Query: 541  HTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCF 720
            HT E  SLREI NS YY + GGEDLK  NALNCNYPIVQQMILESLRYWV EFHIDGFCF
Sbjct: 453  HTGETGSLREIDNSCYYHIKGGEDLKLGNALNCNYPIVQQMILESLRYWVTEFHIDGFCF 512

Query: 721  VNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHWKRWAE 900
            +NASSLTRG HGEFLSRPPL+EAIAFDPLLSKVKIIADSWDPH +ETKE  FPHWKRWAE
Sbjct: 513  INASSLTRGFHGEFLSRPPLVEAIAFDPLLSKVKIIADSWDPHDMETKEIAFPHWKRWAE 572

Query: 901  MNGKFCVDVRNFLRG 945
            MN +FCVDVRNFLRG
Sbjct: 573  MNARFCVDVRNFLRG 587


>ref|XP_011092269.1| isoamylase 2, chloroplastic [Sesamum indicum]
 ref|XP_011092270.1| isoamylase 2, chloroplastic [Sesamum indicum]
          Length = 857

 Score =  546 bits (1408), Expect = 0.0
 Identities = 256/315 (81%), Positives = 283/315 (89%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDFGLDLPRKFLGKLCEEPAF 180
            IWHA +D S+PFV YGYRCR+ T+NKGH VLLDPYA+VIED GL LPRK LGKL +EPA+
Sbjct: 276  IWHASIDSSMPFVSYGYRCRSATDNKGHHVLLDPYAKVIEDLGLGLPRKCLGKLYKEPAY 335

Query: 181  DWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVL 360
            DWSG+F PSLPME+LIIYRLN+T FT +KSSKLPGDIAGTF+G+SEKLHHFK+LGVNA+L
Sbjct: 336  DWSGEFHPSLPMEKLIIYRLNITGFTKNKSSKLPGDIAGTFSGLSEKLHHFKDLGVNAIL 395

Query: 361  LEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFT 540
            LEPIFPFDE+KGPYFPWHFFSP  L GP+GDP TVA+SMKEMVKKLH SGIEVLLEVVFT
Sbjct: 396  LEPIFPFDEKKGPYFPWHFFSPERLCGPTGDPTTVAQSMKEMVKKLHGSGIEVLLEVVFT 455

Query: 541  HTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCF 720
            HT E  +LR+I +SSYY   GG+DL SRNALNCNYPIVQQM+LESLRYWVIEFHIDGFCF
Sbjct: 456  HTGEGCALRDI-DSSYYHTEGGDDLTSRNALNCNYPIVQQMVLESLRYWVIEFHIDGFCF 514

Query: 721  VNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHWKRWAE 900
            +NAS L RG HGEFLSRPPL+EAIAFDP+LSK KIIADSWDPH +ETKE VFPHWKRWAE
Sbjct: 515  INASHLMRGFHGEFLSRPPLVEAIAFDPVLSKAKIIADSWDPHEMETKEIVFPHWKRWAE 574

Query: 901  MNGKFCVDVRNFLRG 945
            MN KFCVDVRNFLRG
Sbjct: 575  MNAKFCVDVRNFLRG 589


>gb|EYU33286.1| hypothetical protein MIMGU_mgv1a001869mg [Erythranthe guttata]
          Length = 747

 Score =  539 bits (1389), Expect = 0.0
 Identities = 255/317 (80%), Positives = 286/317 (90%), Gaps = 2/317 (0%)
 Frame = +1

Query: 1    IWHALVDCS-LPFVGYGYRCRNG-TENKGHRVLLDPYARVIEDFGLDLPRKFLGKLCEEP 174
            IWHA +DCS LPFV YGYRCR+  T++KGHRVL DPYA+VIE+FG   PRK LG+LC E 
Sbjct: 179  IWHASIDCSSLPFVSYGYRCRSSSTDSKGHRVLPDPYAKVIEEFG---PRKCLGRLCNES 235

Query: 175  AFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNA 354
            AFDWSG+ RP+LPME+LI+YRLNV RFT DKSSKLP DIAG+F+GIS+KLH+FKNLGVNA
Sbjct: 236  AFDWSGESRPNLPMEKLIVYRLNVARFTNDKSSKLPRDIAGSFSGISDKLHYFKNLGVNA 295

Query: 355  VLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVV 534
            VLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDP  VA S+KEMVKKLHA+G+EVLLEVV
Sbjct: 296  VLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPSKVANSLKEMVKKLHANGMEVLLEVV 355

Query: 535  FTHTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGF 714
            FTHTSE+++LREI NSSY+ V GGE+LKSRN+LNCNYPIVQQMILE LRYWV EFHIDGF
Sbjct: 356  FTHTSEISALREIDNSSYHHVEGGEELKSRNSLNCNYPIVQQMILECLRYWVTEFHIDGF 415

Query: 715  CFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHWKRW 894
            CF+N+SSLTRG HGEFLSRPPL+EAIAFDPLLSKVKI+ADSWDPH +E KE VFPHWKRW
Sbjct: 416  CFINSSSLTRGFHGEFLSRPPLVEAIAFDPLLSKVKIVADSWDPHEMEMKEIVFPHWKRW 475

Query: 895  AEMNGKFCVDVRNFLRG 945
            AEMN KFC ++RNF RG
Sbjct: 476  AEMNAKFCSEMRNFFRG 492


>ref|XP_012842325.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
 ref|XP_012842326.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
 ref|XP_012842327.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
 ref|XP_012842328.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
          Length = 840

 Score =  539 bits (1389), Expect = 0.0
 Identities = 255/317 (80%), Positives = 286/317 (90%), Gaps = 2/317 (0%)
 Frame = +1

Query: 1    IWHALVDCS-LPFVGYGYRCRNG-TENKGHRVLLDPYARVIEDFGLDLPRKFLGKLCEEP 174
            IWHA +DCS LPFV YGYRCR+  T++KGHRVL DPYA+VIE+FG   PRK LG+LC E 
Sbjct: 272  IWHASIDCSSLPFVSYGYRCRSSSTDSKGHRVLPDPYAKVIEEFG---PRKCLGRLCNES 328

Query: 175  AFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNA 354
            AFDWSG+ RP+LPME+LI+YRLNV RFT DKSSKLP DIAG+F+GIS+KLH+FKNLGVNA
Sbjct: 329  AFDWSGESRPNLPMEKLIVYRLNVARFTNDKSSKLPRDIAGSFSGISDKLHYFKNLGVNA 388

Query: 355  VLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVV 534
            VLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDP  VA S+KEMVKKLHA+G+EVLLEVV
Sbjct: 389  VLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPSKVANSLKEMVKKLHANGMEVLLEVV 448

Query: 535  FTHTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGF 714
            FTHTSE+++LREI NSSY+ V GGE+LKSRN+LNCNYPIVQQMILE LRYWV EFHIDGF
Sbjct: 449  FTHTSEISALREIDNSSYHHVEGGEELKSRNSLNCNYPIVQQMILECLRYWVTEFHIDGF 508

Query: 715  CFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHWKRW 894
            CF+N+SSLTRG HGEFLSRPPL+EAIAFDPLLSKVKI+ADSWDPH +E KE VFPHWKRW
Sbjct: 509  CFINSSSLTRGFHGEFLSRPPLVEAIAFDPLLSKVKIVADSWDPHEMEMKEIVFPHWKRW 568

Query: 895  AEMNGKFCVDVRNFLRG 945
            AEMN KFC ++RNF RG
Sbjct: 569  AEMNAKFCSEMRNFFRG 585


>ref|XP_022895653.1| isoamylase 2, chloroplastic isoform X2 [Olea europaea var.
            sylvestris]
          Length = 829

 Score =  518 bits (1335), Expect = e-176
 Identities = 243/315 (77%), Positives = 279/315 (88%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDFGLDLPRKFLGKLCEEPAF 180
            IWH L+D S+PFV YGYRC++  + K   VLLDPYA++IEDFG  LPRK LGKL +E  F
Sbjct: 248  IWHVLLDGSVPFVRYGYRCKSNADKKRQYVLLDPYAKLIEDFGSGLPRKSLGKLWKESGF 307

Query: 181  DWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVL 360
            DWSGD RPSLPME+LIIYRLNVTRFT D+SSKLPG+I GTFAGISEK+HHFK+LGVNA+L
Sbjct: 308  DWSGDMRPSLPMEKLIIYRLNVTRFTKDESSKLPGEIVGTFAGISEKVHHFKDLGVNAIL 367

Query: 361  LEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFT 540
            LEPIFPFDEQ GPYFPWHFFSPGS YGPSGD + V K MKEMVKKLH +GIEVL+EVVFT
Sbjct: 368  LEPIFPFDEQTGPYFPWHFFSPGSPYGPSGDSEFVVKLMKEMVKKLHLNGIEVLVEVVFT 427

Query: 541  HTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCF 720
            HT+E+A+L++I + SYY V GGEDLKSRNALNCN+PIVQQ+IL+SLR+WVIE+HIDGFCF
Sbjct: 428  HTAEVAALKQI-DRSYYHVGGGEDLKSRNALNCNHPIVQQLILDSLRHWVIEYHIDGFCF 486

Query: 721  VNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHWKRWAE 900
            ++ASSL +G HGEFL+RPPL+EAIAFDPLLSKVKIIADSWDPH +ETK+  FPHWKRWAE
Sbjct: 487  IDASSLMKGFHGEFLTRPPLVEAIAFDPLLSKVKIIADSWDPHNMETKDISFPHWKRWAE 546

Query: 901  MNGKFCVDVRNFLRG 945
            +N KFC DVRNFLRG
Sbjct: 547  INSKFCNDVRNFLRG 561


>ref|XP_022895652.1| isoamylase 2, chloroplastic isoform X1 [Olea europaea var.
            sylvestris]
          Length = 849

 Score =  518 bits (1335), Expect = e-176
 Identities = 243/315 (77%), Positives = 279/315 (88%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDFGLDLPRKFLGKLCEEPAF 180
            IWH L+D S+PFV YGYRC++  + K   VLLDPYA++IEDFG  LPRK LGKL +E  F
Sbjct: 268  IWHVLLDGSVPFVRYGYRCKSNADKKRQYVLLDPYAKLIEDFGSGLPRKSLGKLWKESGF 327

Query: 181  DWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVL 360
            DWSGD RPSLPME+LIIYRLNVTRFT D+SSKLPG+I GTFAGISEK+HHFK+LGVNA+L
Sbjct: 328  DWSGDMRPSLPMEKLIIYRLNVTRFTKDESSKLPGEIVGTFAGISEKVHHFKDLGVNAIL 387

Query: 361  LEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFT 540
            LEPIFPFDEQ GPYFPWHFFSPGS YGPSGD + V K MKEMVKKLH +GIEVL+EVVFT
Sbjct: 388  LEPIFPFDEQTGPYFPWHFFSPGSPYGPSGDSEFVVKLMKEMVKKLHLNGIEVLVEVVFT 447

Query: 541  HTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCF 720
            HT+E+A+L++I + SYY V GGEDLKSRNALNCN+PIVQQ+IL+SLR+WVIE+HIDGFCF
Sbjct: 448  HTAEVAALKQI-DRSYYHVGGGEDLKSRNALNCNHPIVQQLILDSLRHWVIEYHIDGFCF 506

Query: 721  VNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHWKRWAE 900
            ++ASSL +G HGEFL+RPPL+EAIAFDPLLSKVKIIADSWDPH +ETK+  FPHWKRWAE
Sbjct: 507  IDASSLMKGFHGEFLTRPPLVEAIAFDPLLSKVKIIADSWDPHNMETKDISFPHWKRWAE 566

Query: 901  MNGKFCVDVRNFLRG 945
            +N KFC DVRNFLRG
Sbjct: 567  INSKFCNDVRNFLRG 581


>gb|KZV17747.1| isoamylase isoform 2 [Dorcoceras hygrometricum]
          Length = 844

 Score =  518 bits (1333), Expect = e-176
 Identities = 245/315 (77%), Positives = 272/315 (86%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDFGLDLPRKFLGKLCEEPAF 180
            IWHAL+  SLPFV YGYRCR G  N+G  VLLDPYA VI+ FGL L RK+LGKLCEEPAF
Sbjct: 265  IWHALIQDSLPFVSYGYRCRGGIANRGQHVLLDPYATVIKSFGLGLNRKWLGKLCEEPAF 324

Query: 181  DWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAVL 360
            DWSG+ RPSLP+E+LI+YRLNVTRFT DKSSKLP   AGTF GI EKLHHFK+LGVNA+L
Sbjct: 325  DWSGESRPSLPLEKLIVYRLNVTRFTKDKSSKLPTSAAGTFYGIYEKLHHFKDLGVNAIL 384

Query: 361  LEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFT 540
            LEPIFP DE+K PYFPWHFFSPGS YGPS D   + KSMKEMVKKLHASGIEVLLEVVFT
Sbjct: 385  LEPIFPSDERKEPYFPWHFFSPGSSYGPSDDLSYIVKSMKEMVKKLHASGIEVLLEVVFT 444

Query: 541  HTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCF 720
            HT E  +L+ I  S Y+S+   EDLKS+N LNCNYP+VQQ+ILESLR+WVIE+HIDGFCF
Sbjct: 445  HTGEDVTLKGIDESYYHSI---EDLKSKNMLNCNYPVVQQLILESLRHWVIEYHIDGFCF 501

Query: 721  VNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHWKRWAE 900
            +NASSL RG HGEFLSRPPL+EAIAFDPLLS++KIIADSWDPHG+E+KE VFPHWKRWAE
Sbjct: 502  INASSLLRGKHGEFLSRPPLVEAIAFDPLLSQIKIIADSWDPHGMESKELVFPHWKRWAE 561

Query: 901  MNGKFCVDVRNFLRG 945
            MNGKFC D RNFLRG
Sbjct: 562  MNGKFCADARNFLRG 576


>ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum]
 gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]
          Length = 878

 Score =  473 bits (1218), Expect = e-158
 Identities = 226/320 (70%), Positives = 266/320 (83%), Gaps = 5/320 (1%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFLGKLC 165
            IWHA +DCSLPF  YGYRC+  T  KG  VLLDPYA+VI        G ++  K+LG+LC
Sbjct: 275  IWHAALDCSLPFKTYGYRCKATTSGKGELVLLDPYAKVIRRVIPRQGGSEIRPKYLGELC 334

Query: 166  EEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLG 345
             EP +DWSGD  PSLPME+LIIYRLNVT+FT DKSSKLP D+AGTF+GISEK HHFK+LG
Sbjct: 335  LEPGYDWSGDVPPSLPMEKLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLG 394

Query: 346  VNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLL 525
            VNA+LLEPIFPFDEQKGPYFPWHFFSPG++YGPSGDP +  KSMK+MVKKLHA+GIEV L
Sbjct: 395  VNAMLLEPIFPFDEQKGPYFPWHFFSPGNMYGPSGDPLSAIKSMKDMVKKLHANGIEVFL 454

Query: 526  EVVFTHTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHI 705
            EVVFTHT+E A L  + N S Y + GG+ L  +NALNCNYPIVQQMIL+ LR+WVIEFHI
Sbjct: 455  EVVFTHTAEDAPLMNVDNFS-YCIKGGQYLNIQNALNCNYPIVQQMILDCLRHWVIEFHI 513

Query: 706  DGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHW 885
            DGF FVNASSL RG +GE LSRPPL+EAIAFDP+LSKVK+IAD+W+P   ++KE +FPHW
Sbjct: 514  DGFVFVNASSLLRGFNGEILSRPPLVEAIAFDPILSKVKMIADNWNPLTNDSKENLFPHW 573

Query: 886  KRWAEMNGKFCVDVRNFLRG 945
            +RWAE+N +FC D+R+FLRG
Sbjct: 574  RRWAEINMRFCDDIRDFLRG 593


>ref|XP_006355876.1| PREDICTED: isoamylase isoform 2 isoform X1 [Solanum tuberosum]
 ref|XP_015168040.1| PREDICTED: isoamylase isoform 2 isoform X1 [Solanum tuberosum]
          Length = 878

 Score =  473 bits (1218), Expect = e-158
 Identities = 226/320 (70%), Positives = 266/320 (83%), Gaps = 5/320 (1%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFLGKLC 165
            IWHA +DCSLPF  YGYRC+  T  KG  VLLDPYA+VI        G ++  K+LG+LC
Sbjct: 275  IWHAALDCSLPFKTYGYRCKATTSGKGELVLLDPYAKVIRRVIPRQGGSEIRPKYLGELC 334

Query: 166  EEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLG 345
             EP +DWSGD  PSLPME+LIIYRLNVT+FT DKSSKLP D+AGTF+GISEK HHFK+LG
Sbjct: 335  LEPGYDWSGDVPPSLPMEKLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLG 394

Query: 346  VNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLL 525
            VNA+LLEPIFPFDEQKGPYFPWHFFSPG++YGPSGDP +  KSMK+MVKKLHA+GIEV L
Sbjct: 395  VNAMLLEPIFPFDEQKGPYFPWHFFSPGNMYGPSGDPLSAIKSMKDMVKKLHANGIEVFL 454

Query: 526  EVVFTHTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHI 705
            EVVFTHT+E A L  + N S Y + GG+ L  +NALNCNYPIVQQMIL+ LR+WVIEFHI
Sbjct: 455  EVVFTHTAEDAPLMNVDNFS-YCIKGGQYLNIQNALNCNYPIVQQMILDCLRHWVIEFHI 513

Query: 706  DGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHW 885
            DGF FVNASSL RG +GE LSRPPL+EAIAFDP+LSKVK+IAD+W+P   ++KE +FPHW
Sbjct: 514  DGFVFVNASSLLRGFNGEILSRPPLVEAIAFDPILSKVKMIADNWNPLTNDSKENLFPHW 573

Query: 886  KRWAEMNGKFCVDVRNFLRG 945
            +RWAE+N +FC D+R+FLRG
Sbjct: 574  RRWAEINMRFCDDIRDFLRG 593


>dbj|BAW33107.1| isoamylase [Ipomoea batatas]
          Length = 865

 Score =  470 bits (1209), Expect = e-157
 Identities = 219/320 (68%), Positives = 261/320 (81%), Gaps = 5/320 (1%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFLGKLC 165
            +WHA +D SLPFVGYGYRC+  T  K    LLDPYA+++ D+     G +L  +FLG+LC
Sbjct: 277  VWHASIDGSLPFVGYGYRCKGATAEKTEHALLDPYAKILVDYIPPREGFNLVPRFLGQLC 336

Query: 166  EEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLG 345
            +EPAFDWS D RP++PME+LI+Y LNV+ FT DKSSKLP  +AGTF+GI+EKL HFK+LG
Sbjct: 337  KEPAFDWSNDIRPNIPMEKLIVYHLNVSNFTKDKSSKLPAQVAGTFSGITEKLQHFKDLG 396

Query: 346  VNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLL 525
            VNA+LLEPIFPFDEQKGPYFP HFFSPG L GP GDP ++  SMK+MVK++HA+GIEV L
Sbjct: 397  VNAILLEPIFPFDEQKGPYFPVHFFSPGHLCGPPGDPSSIINSMKQMVKEVHANGIEVFL 456

Query: 526  EVVFTHTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHI 705
            +VVFTHT+E ASL  I  SSYY +     +KS N+LNCNYP VQQ+IL+SLR+WV+E+HI
Sbjct: 457  QVVFTHTAEGASLLHIDKSSYYYLEDDMGMKSGNSLNCNYPPVQQLILDSLRHWVVEYHI 516

Query: 706  DGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHW 885
            DGFCFVNAS LTRG HGEFL+ PPLIEAIAFDPLLSKVK+IAD WDP    +KE +FPHW
Sbjct: 517  DGFCFVNASFLTRGFHGEFLTHPPLIEAIAFDPLLSKVKLIADFWDPVEKTSKEIIFPHW 576

Query: 886  KRWAEMNGKFCVDVRNFLRG 945
            KRWAE+N KFC DVRNFLRG
Sbjct: 577  KRWAEINSKFCDDVRNFLRG 596


>ref|XP_019161030.1| PREDICTED: isoamylase 2, chloroplastic [Ipomoea nil]
          Length = 865

 Score =  470 bits (1209), Expect = e-157
 Identities = 221/320 (69%), Positives = 260/320 (81%), Gaps = 5/320 (1%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFLGKLC 165
            IWHA +D SLPFVGYGYRC+  T  K   VLLDPYA++I D+     G +L  + LG+LC
Sbjct: 277  IWHASIDGSLPFVGYGYRCKGATAEKTEHVLLDPYAKIIVDYNPPGEGFNLVPRCLGQLC 336

Query: 166  EEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLG 345
            ++PAFDWS D RP++PME L++Y LNV  FT DKSSKLP  +AGTF+GI+EKL HFK++G
Sbjct: 337  KKPAFDWSNDIRPNIPMETLVVYHLNVCNFTKDKSSKLPAQVAGTFSGITEKLQHFKDIG 396

Query: 346  VNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLL 525
            VNA+LLEPIFPFDEQKGPYFP HFFSPG L GP GDP +V  SMK+MVK++HA+GIEV L
Sbjct: 397  VNAILLEPIFPFDEQKGPYFPMHFFSPGHLCGPPGDPSSVINSMKQMVKEVHANGIEVFL 456

Query: 526  EVVFTHTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHI 705
            +VVFTHT+E ASL  I  SSYY + G   +KS N+LNCNYP VQQMIL+SLR+WV+E+HI
Sbjct: 457  QVVFTHTAEGASLLHIDKSSYYYLEGDMGMKSGNSLNCNYPPVQQMILDSLRHWVVEYHI 516

Query: 706  DGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHW 885
            DGFCFVNAS LTRG HGEFL+ PPLIEAIAFDPLLSKVK+IAD WDP    +KE +FPHW
Sbjct: 517  DGFCFVNASFLTRGFHGEFLTHPPLIEAIAFDPLLSKVKLIADFWDPVEKTSKEIIFPHW 576

Query: 886  KRWAEMNGKFCVDVRNFLRG 945
            KRWAE+N KFC DVRNFLRG
Sbjct: 577  KRWAEINSKFCDDVRNFLRG 596


>gb|AOQ26242.1| ISA2 [Actinidia deliciosa]
          Length = 866

 Score =  466 bits (1198), Expect = e-155
 Identities = 220/320 (68%), Positives = 255/320 (79%), Gaps = 5/320 (1%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENKG-----HRVLLDPYARVIEDFGLDLPRKFLGKLC 165
            IWH  +D +LPFV YGYRC+ G   KG       VLLDPYA+VI +       K LGKL 
Sbjct: 275  IWHVSMDSALPFVSYGYRCKGGIHGKGVKDHSEHVLLDPYAKVIANAHSGSVLKTLGKLI 334

Query: 166  EEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLG 345
            +EP FDWSGD RP +PME+L++YR+NV  FT  KSS LP D+AG+F GI+EKL H K+LG
Sbjct: 335  KEPTFDWSGDIRPCIPMEKLVVYRVNVAHFTKHKSSGLPNDVAGSFTGITEKLQHCKDLG 394

Query: 346  VNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLL 525
            VNA+LLEP+FPFDE+KGPYFP+HFFSP  LYGPS DP     SMKEMVK LHA+GIEVLL
Sbjct: 395  VNAILLEPVFPFDEKKGPYFPFHFFSPMKLYGPSDDPSATVNSMKEMVKILHANGIEVLL 454

Query: 526  EVVFTHTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHI 705
            E+VFTHT+E  SLREI  SSYY + G EDL ++NALNCNYPIVQQMIL+SLRYW IEFH+
Sbjct: 455  EIVFTHTAESCSLREIDKSSYY-ITGDEDLGAKNALNCNYPIVQQMILDSLRYWAIEFHV 513

Query: 706  DGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHW 885
            DGFCF+NASSL +G HGEFLSRPPL+EAIAFDPLLSK K+IADSWDPH + + E +FPHW
Sbjct: 514  DGFCFINASSLLKGFHGEFLSRPPLVEAIAFDPLLSKTKLIADSWDPHDMASDEILFPHW 573

Query: 886  KRWAEMNGKFCVDVRNFLRG 945
            KRWAE+N  FC DVRNFLRG
Sbjct: 574  KRWAEVNTNFCGDVRNFLRG 593


>ref|XP_004247144.2| PREDICTED: isoamylase 2, chloroplastic [Solanum lycopersicum]
          Length = 866

 Score =  460 bits (1184), Expect = e-153
 Identities = 218/320 (68%), Positives = 262/320 (81%), Gaps = 5/320 (1%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFLGKLC 165
            IWHA +DCSLPF  YGYR +  T  KG  VLLDPYA+VI        G ++  K+LG+LC
Sbjct: 275  IWHAALDCSLPFKTYGYRFKAATSGKGELVLLDPYAKVIRSVIPRQGGSEIRPKYLGELC 334

Query: 166  EEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLG 345
             EP +DWSGD  PSLPME+LIIYR+NVT FT DKSSKLP ++AGTF+GISEK HH K+LG
Sbjct: 335  LEPGYDWSGDVPPSLPMEKLIIYRINVTHFTKDKSSKLPDNLAGTFSGISEKWHHLKDLG 394

Query: 346  VNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLL 525
            VNA+LLEPIFPFDEQKGPY+PWHFFSPG++YGPSGDP +V KSMK+MVKKLHA+GIEV L
Sbjct: 395  VNAMLLEPIFPFDEQKGPYYPWHFFSPGNMYGPSGDPLSVIKSMKDMVKKLHANGIEVFL 454

Query: 526  EVVFTHTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHI 705
            EVVFTHT+E A L  + N S Y + G +D+  +NALNCNYPIVQQMIL+ LR+WVIEFHI
Sbjct: 455  EVVFTHTAEDAPLMNVDNFS-YCIKGSQDMNIQNALNCNYPIVQQMILDCLRHWVIEFHI 513

Query: 706  DGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHW 885
            DGF FV+ASSL RG +GE LSRPPL+EAIAFDP+LSK K+IAD+W+P   ++ E +FPHW
Sbjct: 514  DGFVFVDASSLLRGFNGEILSRPPLVEAIAFDPILSKAKMIADNWNPLTNDSTENLFPHW 573

Query: 886  KRWAEMNGKFCVDVRNFLRG 945
            +RWAE+N +FC D+R+FLRG
Sbjct: 574  RRWAEINMRFCDDIRDFLRG 593


>ref|XP_015087879.1| PREDICTED: isoamylase 2, chloroplastic [Solanum pennellii]
          Length = 866

 Score =  458 bits (1179), Expect = e-152
 Identities = 219/320 (68%), Positives = 262/320 (81%), Gaps = 5/320 (1%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFLGKLC 165
            IWHA +DCSLPF  YGYR +  T  KG  VLLDPYA+VI        G ++  K+LG+LC
Sbjct: 275  IWHAALDCSLPFKTYGYRFKAATSGKGELVLLDPYAKVIRSVIPRQGGSEIRPKYLGELC 334

Query: 166  EEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLG 345
             EP +DWSGD  PSLPME+LIIYR+NVT FT DKSSKLP ++AGTF+GISEK HH K+LG
Sbjct: 335  LEPGYDWSGDVPPSLPMEKLIIYRINVTHFTKDKSSKLPDNLAGTFSGISEKWHHLKDLG 394

Query: 346  VNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLL 525
            VNA+LLEPIFPFDEQKGPY+PWHFFSPG++YGPSGDP +V K+MK+MVKKLHA+GIEV L
Sbjct: 395  VNAMLLEPIFPFDEQKGPYYPWHFFSPGNMYGPSGDPLSVIKTMKDMVKKLHANGIEVFL 454

Query: 526  EVVFTHTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHI 705
            EVVFTHT+E A L  + N S Y + GG+DL  +NALNCN PIVQQMIL+ LR+WVIEFHI
Sbjct: 455  EVVFTHTAEDAPLMNVDNFS-YCIKGGQDLNIQNALNCNCPIVQQMILDCLRHWVIEFHI 513

Query: 706  DGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHW 885
            DGF FVNASSL RG +GE LSRPPL+EAIAFD +LSKVK+IAD+W+P   ++ E +FPHW
Sbjct: 514  DGFVFVNASSLLRGFNGEILSRPPLVEAIAFDLILSKVKMIADNWNPLTNDSTENLFPHW 573

Query: 886  KRWAEMNGKFCVDVRNFLRG 945
            +RWAE+N +FC D+R+FLRG
Sbjct: 574  RRWAEINMRFCDDIRDFLRG 593


>ref|XP_016450982.1| PREDICTED: isoamylase 2, chloroplastic-like [Nicotiana tabacum]
          Length = 863

 Score =  456 bits (1172), Expect = e-151
 Identities = 219/320 (68%), Positives = 259/320 (80%), Gaps = 5/320 (1%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFLGKLC 165
            IWHA +DCSLP   YGYRC+  T  K   VLLDPY++VI        G  L  K+LG+LC
Sbjct: 276  IWHAALDCSLPIKTYGYRCKVATSGKEELVLLDPYSKVIRSVIPRQGGSKLRPKYLGELC 335

Query: 166  EEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLG 345
            +EP +DWSGD  PSL ME+LIIYRLNVT FT D SSKLP D+AGTF+GI+EK  HFK+LG
Sbjct: 336  KEPGYDWSGDVPPSLHMEKLIIYRLNVTEFTNDGSSKLPDDLAGTFSGITEKWSHFKHLG 395

Query: 346  VNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLL 525
            VNA+LLEPIFPF+EQKGPYFP HFFSPG+++G SGDP +V KSMK+MVKKLHA+GIE+ L
Sbjct: 396  VNAILLEPIFPFEEQKGPYFPSHFFSPGNMHGHSGDPLSVIKSMKDMVKKLHANGIEIFL 455

Query: 526  EVVFTHTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHI 705
            EVVFTHT+E A L  + NSSYYS+ GG DL  + ALNCNYPIVQQMIL+ LRYWVIEFHI
Sbjct: 456  EVVFTHTAEDAPLMHVDNSSYYSIKGG-DLNIQYALNCNYPIVQQMILDCLRYWVIEFHI 514

Query: 706  DGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHW 885
            DGF F+NASSL +G HGE LSRPPL+EAIAFDP+LSKVKIIAD WD    ++KE +FPHW
Sbjct: 515  DGFVFINASSLLKGFHGEILSRPPLVEAIAFDPILSKVKIIADYWDALANDSKEILFPHW 574

Query: 886  KRWAEMNGKFCVDVRNFLRG 945
            ++WAE+N +FC D+R+FLRG
Sbjct: 575  RKWAEINKRFCDDIRDFLRG 594


>ref|XP_019231687.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana attenuata]
 gb|OIT28559.1| isoamylase 2, chloroplastic [Nicotiana attenuata]
          Length = 863

 Score =  455 bits (1170), Expect = e-151
 Identities = 218/320 (68%), Positives = 259/320 (80%), Gaps = 5/320 (1%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFLGKLC 165
            IWHA +DCSLPF  YGYRC+  T  K   VLLDPY++VI        G  L  K+LG+LC
Sbjct: 276  IWHAALDCSLPFKTYGYRCKVATSGKEEMVLLDPYSKVIRSVIPRQGGSKLRPKYLGELC 335

Query: 166  EEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLG 345
            +EP +DWSGD  PSLP+E LIIYRLNVT FT D SSKLP D+AGTF+GI+EKL HFK+LG
Sbjct: 336  KEPGYDWSGDVPPSLPLENLIIYRLNVTEFTKDGSSKLPDDLAGTFSGITEKLSHFKHLG 395

Query: 346  VNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLL 525
            VNA+LLEPIFPF+EQKGPYFP HFFSPG+++G S DP +V  SMK+MVKKLHA+GIE+ L
Sbjct: 396  VNAILLEPIFPFEEQKGPYFPSHFFSPGNMHGRSSDPLSVINSMKDMVKKLHANGIEIFL 455

Query: 526  EVVFTHTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHI 705
            EVVFTH +E A L  + NSS+YS+ GG DL  + ALNCNYPIVQQMIL+ LRYWVIEFHI
Sbjct: 456  EVVFTHIAEDAPLMHVDNSSHYSIKGG-DLNIQYALNCNYPIVQQMILDCLRYWVIEFHI 514

Query: 706  DGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHW 885
            DGF FVNASSL++G HGE LSRPPL+EAIAFDP+LSKVKIIAD WD    ++KE +FPHW
Sbjct: 515  DGFVFVNASSLSKGFHGEILSRPPLVEAIAFDPILSKVKIIADYWDALANDSKEILFPHW 574

Query: 886  KRWAEMNGKFCVDVRNFLRG 945
            ++WAE+N +FC D+R+FLRG
Sbjct: 575  RKWAEINTRFCDDIRDFLRG 594


>gb|AAY89379.1| isoamylase isoform 2, partial [Nicotiana langsdorffii x Nicotiana
            sanderae]
          Length = 556

 Score =  444 bits (1141), Expect = e-151
 Identities = 217/320 (67%), Positives = 256/320 (80%), Gaps = 5/320 (1%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENKGHRVLLDPYARVIEDF-----GLDLPRKFLGKLC 165
            IWHA +DCSLPF  YGYRC+  T  K   VLLDPY++VI        G +L  K+LG+LC
Sbjct: 139  IWHAALDCSLPFKTYGYRCKVATSGKEELVLLDPYSKVIRSVIPRQGGSELRPKYLGELC 198

Query: 166  EEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLG 345
            +EP +DWSGD  PSLPME+LIIYRLNVT FT D SSKLP D+AGTF+GI+EKL HFK+LG
Sbjct: 199  KEPGYDWSGDVPPSLPMEKLIIYRLNVTEFTKDGSSKLPDDLAGTFSGITEKLSHFKHLG 258

Query: 346  VNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLL 525
            VNAVLLEPIFPF+EQKGPYFP HFFSPG+++G S DP +V  SM++MVKKLHA+G E+ L
Sbjct: 259  VNAVLLEPIFPFEEQKGPYFPSHFFSPGNMHGRSSDPLSVINSMEDMVKKLHANGKEIFL 318

Query: 526  EVVFTHTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHI 705
            EVVFTHT+E A L  + NSSY S+ GG DL  + ALNCNYPIVQQMIL  LR+WVIEFHI
Sbjct: 319  EVVFTHTAEDAPLMHVDNSSYCSIKGG-DLNIQYALNCNYPIVQQMILGCLRHWVIEFHI 377

Query: 706  DGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPHW 885
             GF FVNASSL +G  GE LSRPPLIEAIAFDP+LSKVKIIAD WD    ++KE +FPHW
Sbjct: 378  GGFVFVNASSLLKGFLGEILSRPPLIEAIAFDPILSKVKIIADYWDALANDSKEILFPHW 437

Query: 886  KRWAEMNGKFCVDVRNFLRG 945
            ++WAE+N +FC D+R+FLRG
Sbjct: 438  RKWAEINTRFCDDIRDFLRG 457


>gb|PHU26272.1| Isoamylase 2, chloroplastic [Capsicum chinense]
          Length = 862

 Score =  452 bits (1164), Expect = e-150
 Identities = 218/321 (67%), Positives = 264/321 (82%), Gaps = 6/321 (1%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENK-GHRVLLDPYARVIEDF-----GLDLPRKFLGKL 162
            IWHA +DCSL F  YGYRC+  T  K G  VLLDPYA+VI        G +   K+LG+L
Sbjct: 275  IWHAALDCSLLFKTYGYRCKVATSGKRGELVLLDPYAKVIRSVISRQGGSEKCPKYLGEL 334

Query: 163  CEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNL 342
            C+EP +DWSGD RPSLPME+LIIYRLNVT+FT DKSSKLP D+AGTF+GIS+K HHFK+L
Sbjct: 335  CQEPGYDWSGDVRPSLPMEKLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISKKWHHFKDL 394

Query: 343  GVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVL 522
            GVNA+LL+PIFPF+EQKGPY+P HFFSPG++YG SGD  +V KS+K+MVKKLHA+GIEV 
Sbjct: 395  GVNAMLLQPIFPFEEQKGPYYPCHFFSPGNMYGRSGDALSVIKSVKDMVKKLHANGIEVF 454

Query: 523  LEVVFTHTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFH 702
            LEVVFTHT++ A L  + N+SYY + GG DL  +NALNCNYP+VQQMIL+ LR+WVIEF 
Sbjct: 455  LEVVFTHTAQDAPLMHVDNTSYY-IKGG-DLNIQNALNCNYPVVQQMILDCLRHWVIEFR 512

Query: 703  IDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPH 882
            IDGF F+NASSL RG HGE LSRPPL+EAIAFDP+LS VKIIAD+W+P   ++KE +FPH
Sbjct: 513  IDGFVFINASSLLRGFHGEILSRPPLVEAIAFDPILSNVKIIADNWNPVANDSKENLFPH 572

Query: 883  WKRWAEMNGKFCVDVRNFLRG 945
            W+RWAE+N +FC D+R+FLRG
Sbjct: 573  WRRWAEINLRFCADIRDFLRG 593


>gb|PHT89964.1| Isoamylase 2, chloroplastic [Capsicum annuum]
          Length = 862

 Score =  450 bits (1158), Expect = e-149
 Identities = 218/321 (67%), Positives = 263/321 (81%), Gaps = 6/321 (1%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENK-GHRVLLDPYARVIEDF-----GLDLPRKFLGKL 162
            IWHA +DCSL F  YGYRC+  T  K G  VLLDPYA+VI        G +   K+LG+L
Sbjct: 275  IWHAALDCSLLFKTYGYRCKAATSGKRGELVLLDPYAKVIRSVISRQGGSEKCPKYLGEL 334

Query: 163  CEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNL 342
            C+EP +DWSGD RPSLPME+LIIYRLNVT+FT DKSSKLP D+AGTF+GIS+K HHFK+L
Sbjct: 335  CQEPGYDWSGDVRPSLPMEKLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISKKWHHFKDL 394

Query: 343  GVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVL 522
            GVNA+LL+PIFPF+EQKGPY+P HFFSPG++YG SGD  +V KS+K+MVKKLHA+GIEV 
Sbjct: 395  GVNAMLLQPIFPFEEQKGPYYPCHFFSPGNMYGRSGDALSVIKSVKDMVKKLHANGIEVF 454

Query: 523  LEVVFTHTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFH 702
            LEVVFTHT++ A L  + N+S Y V GG DL  +NALNCNYP+VQQMIL+ LR+WVIEF 
Sbjct: 455  LEVVFTHTAQDAPLMHVDNTS-YCVKGG-DLNIQNALNCNYPVVQQMILDCLRHWVIEFR 512

Query: 703  IDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPH 882
            IDGF F+NASSL RG HGE LSRPPL+EAIAFDP+LS VKIIAD+W+P   ++KE +FPH
Sbjct: 513  IDGFVFINASSLLRGFHGEILSRPPLVEAIAFDPILSNVKIIADNWNPVANDSKENLFPH 572

Query: 883  WKRWAEMNGKFCVDVRNFLRG 945
            W+RWAE+N +FC D+R+FLRG
Sbjct: 573  WRRWAEINLRFCADIRDFLRG 593


>ref|XP_016559778.1| PREDICTED: isoamylase 2, chloroplastic-like [Capsicum annuum]
 ref|XP_016559779.1| PREDICTED: isoamylase 2, chloroplastic-like [Capsicum annuum]
 ref|XP_016559780.1| PREDICTED: isoamylase 2, chloroplastic-like [Capsicum annuum]
          Length = 862

 Score =  450 bits (1158), Expect = e-149
 Identities = 218/321 (67%), Positives = 263/321 (81%), Gaps = 6/321 (1%)
 Frame = +1

Query: 1    IWHALVDCSLPFVGYGYRCRNGTENK-GHRVLLDPYARVIEDF-----GLDLPRKFLGKL 162
            IWHA +DCSL F  YGYRC+  T  K G  VLLDPYA+VI        G +   K+LG+L
Sbjct: 275  IWHAALDCSLLFKTYGYRCKAATSGKRGELVLLDPYAKVIRSVISRQGGSEKCPKYLGEL 334

Query: 163  CEEPAFDWSGDFRPSLPMERLIIYRLNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNL 342
            C+EP +DWSGD RPSLPME+LIIYRLNVT+FT DKSSKLP D+AGTF+GIS+K HHFK+L
Sbjct: 335  CQEPGYDWSGDVRPSLPMEKLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISKKWHHFKDL 394

Query: 343  GVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVL 522
            GVNA+LL+PIFPF+EQKGPY+P HFFSPG++YG SGD  +V KS+K+MVKKLHA+GIEV 
Sbjct: 395  GVNAMLLQPIFPFEEQKGPYYPCHFFSPGNMYGRSGDALSVIKSIKDMVKKLHANGIEVF 454

Query: 523  LEVVFTHTSELASLREIYNSSYYSVNGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFH 702
            LEVVFTHT++ A L  + N+S Y V GG DL  +NALNCNYP+VQQMIL+ LR+WVIEF 
Sbjct: 455  LEVVFTHTAQDAPLMHVDNTS-YCVKGG-DLNIQNALNCNYPVVQQMILDCLRHWVIEFR 512

Query: 703  IDGFCFVNASSLTRGGHGEFLSRPPLIEAIAFDPLLSKVKIIADSWDPHGVETKETVFPH 882
            IDGF F+NASSL RG HGE LSRPPL+EAIAFDP+LS VKIIAD+W+P   ++KE +FPH
Sbjct: 513  IDGFVFINASSLLRGFHGEILSRPPLVEAIAFDPILSNVKIIADNWNPVANDSKENLFPH 572

Query: 883  WKRWAEMNGKFCVDVRNFLRG 945
            W+RWAE+N +FC D+R+FLRG
Sbjct: 573  WRRWAEINLRFCADIRDFLRG 593